Digital Commons Network^™

Leptin And The Control Of Body Weight And Metabolism: Role For Stearoyl Coa Desaturase-1 And Other Leptin-Regulated Genes In The Liver, Paul Cohen

Student Theses and Dissertations

Leptin is an adipocyte-derived hormone that regulates energy balance, metabolism, and the neuroendocrine response to altered nutrition. Mice lacking leptin (ob/ob) or its receptor (db/db) are massively obese and hyperphagic. They also have increased triglyceride deposits in multiple peripheral organs, including the liver, massively enlarged and steatotic. Leptin elicits a metabolic response that cannot be explained by its anorectic effects alone. This response is characterized by weight loss, depletion of lipid from peripheral tissues, and enhanced insulin sensitivity. The mechanisms underlying these actions were largely unknown. This thesis addressed leptin's metabolic effects on the liver, an organ with a critical …

Nova Regulation Of Alternative Splicing In The Cns, B Kate Dredge

Student Theses and Dissertations

Nova-1, an autoantigen in paraneoplastic opsoclonus myoclonus ataxia (POMA), a disorder associated with gynecologic cancer and CNS motor dysfunction, is a neuronspecific nuclear RNA binding protein. The work presented here confirms that Nova-1 acts as a regulator of alternative splicing in vivo. In addition to the known target Nova-dependent splicing regulation, exon 3A of glycine receptor α2 subunit (GlyRα2), two new targets are identified, which are the alternative exon, E9 in the inhibitory neurotransmitter receptor subunit, GABAARγ2, and the alternatively spliced exon H in Nova-1's own message. Nova acts to enhance alternative exon inclusion in GlyRα2 and GABAARγ2 mRNAs and …

Zipro1: Fine Tuning Proliferation In Discrete Cell Populations, Christopher Wynder

Student Theses and Dissertations

Zipro 1 is a zinc finger transcription factor that has a restricted pattern of expression in several tissues. The main cells expressing Zipro1 are proliferating cells from epidermal lineages. This includes the cerebellar granule cells (GCs), nasal epithelia and dermal papilla. Through gene dosage experiments we have shown that Zipro1 is involved in proliferation GCs, Hair follicles and the Cranio-facial process. Overexpression of Zipro1 results an increase in the number of GCs, this increase in GCs directly regulates the appearance of secondary fissure in the cerebellum. Overexpression also results in follicular dysplasia caused by an increase in cells within dermal …

Site-Specific Incorporation Of Biochemical And Biophysical Probes Into Proteins Using Expressed Protein Ligation, Mande Holford

Student Theses and Dissertations

Protein engineering can be made far more powerful if a protein is not only expressed recombinantly but also altered covalently using synthetic chemistry. These two methods are brought together in the protein semi-synthesis technique Expressed Protein Ligation (EPL). In EPL, recombinant and synthetic polypeptides are joined together via a chemoselective ligation reaction. EPL was originally used to attach synthetic constructs to the C-terminus of recombinant proteins, but is now used to attach recombinant or synthetic polypeptides either at the N- or C-terminus of a protein or into the core of a protein. This thesis illustrates, with three distinct applications, the …

Bilbies, Salinity And A Question Of Balance, Gael Bell, Bruce Radys, John Goodlad, Joy Kilian

Books & book chapters

This book is designed to be used in conjunction with the manual 'Salinity in the classroom'.

Published with the assistance of Greening Australia Western Australia, Department of Conservation and Land Management, Department of Education Western Australia.

The Osteopetrotic Mutation Toothless (Tl) Is A Loss-Of-Function Frameshift Mutation In The Rat Csf1 Gene: Evidence Of A Crucial Role For Csf-1 In Osteoclastogenesis And Endochondral Ossification, Liesbeth Van Wesenbeeck, Paul R. Odgren, Carole A. Mackay, Marina D'Angelo, Fayez F. Safadi, Steven N. Popoff, Wim Van Hul, Sandy C. Marks Jr.

PCOM Scholarly Works

The toothless (tl) mutation in the rat is a naturally occurring, autosomal recessive mutation resulting in a profound deficiency of bone-resorbing osteoclasts and peritoneal macrophages. The failure to resorb bone produces severe, unrelenting osteopetrosis, with a highly sclerotic skeleton, lack of marrow spaces, failure of tooth eruption, and other pathologies. Injections of CSF-1 improve some, but not all, of these. In this report we have used polymorphism mapping, sequencing, and expression studies to identify the genetic lesion in the tl rat. We found a 10-base insertion near the beginning of the open reading of the Csf1 gene that yields a …

Digital Color - Where Is The Market?, Barbara Pellow, Franziska Frey, Patricia Sorce

Books

Digital color was introduced in the marketplace almost a decade ago. Both Indigo and Xeikon introduced key new products, and early projections were that these technologies would take off. Initially, as with a number of new technologies, there were technical issues. Presses were unreliable; ink and toner didn’t stick to the paper; and the cost of consumables was too high to generate any substantial application transfer from offset technology. Today, Indigo has been taken over by HP. Xeikon faced bankruptcy and was acquired by Punch Technologies. And we are seeing the emergence of new suppliers for production color: Xerox with …

The Generation Beyond Print-On-Paper, Michael Kleper

Books

The purpose of this paper is to: • Compare and contrast paper display with on-screen display. • Examine the state-of-the-art in electronic “paper-like” screen display technology. • Highlight the screen display technologies that are under development and most likely to affect or replace print. • Introduce the subject of printed electronics. • Present comments from leaders in the display technology field. Today’s printing and publishing process involves repurposing content, publishing through multiple channels, and applying XML coding to separate form from content. All of these operations can serve, in one way or another, to disassociate print output from other forms …

Easytrip Information System (Etis) Backend System / Ching Yu Yu, Yu Yu Ching

Student Works (2000-2009)

Easytrip Travel Information System (ETIS) backend processing is my final year thesis project title. ETIS is a Web-based travel information system that build for the local hotels, restaurant and transportation company to promote their services. It also enables the World Wide Web user to get the complete travel information in Malaysia ETIS backend system consists eight modules. There are Member registration and sign in module, Accommodations reservation module, E-partner module, Administrator management module, Report module, Newsletter generating module and Help system module. These modules enable the web administrator to manage membership and partnership leveraging on Internet infrastructure. With this capability, …

One Man's Trash Is Another Man's Treasure, Bioprospecting: Protecting The Rights And Interests Of Human Donors Of Genetic Material, Anne Nichols Hill

Journal of Health Care Law and Policy

No abstract provided.

Regulation Of Research With Children: The Evolution From Exclusion To Inclusion, Duane Alexander

Journal of Health Care Law and Policy

No abstract provided.

Reptiles In The Weeds: Civil Rico Vs. The First Amendment In The Animal Rights Debate, Jaime I. Roth

University of Miami Law Review

No abstract provided.

Table Of Contents

University of Miami Law Review

No abstract provided.

Putting The Precautionary Principle In Its Place: Parameters For The Proper Application Of A Precautionary Approach And The Implications For Developing Countries In Light Of The Doha Wto Ministerial, Laurent A. Ruessmann

American University International Law Review

No abstract provided.

Activation Of Central Melanocortin Pathways By Fenfluramlne, Lora K. Heisler, Michael A. Cowley, Laurence H. Tecott, Wei Fan, Malcolm J. Low, James L. Smart, Marcelo Rubinstein, Jeffrey B. Tatro, Jacob N. Marcus, Henne Holstege, Charlotte E. Lee, Roger D. Cone, Joel K. Elmquist

Faculty Publications - Department of Biological & Molecular Science

D-fenfluramine (d-FEN) was once widely prescribed and was among the most effective weight loss drugs, but was withdrawn from clinical use because of reports of cardiac complications in a subset of patients. Discerning the neurobiology underlying the anorexic action of d-FEN may facilitate the development of new drugs to prevent and treat obesity. Through a combination of functional neuroanatomy, feeding, and electrophysiology studies in rodents, we show that d-FEN-induced anorexia requires activation of central nervous system melanocortin pathways. These results provide a mechanistic explanation of d-FEN's anorexic actions and indicate that drugs targeting these downstream melanocortin pathways may prove to …

A Complex, Cross-Taxon, Chemical Releaser Of Antipredator Behavior In Amphibians, Dale M. Madison, Aaron M. Sullivan, John C. Maerz, James H. Mcdarby, Jason R. Rohr

Faculty Publications - Department of Biological & Molecular Science

Prey species show diverse antipredator responses to chemical cues signaling predation threat. Among terrestrial vertebrates, the red-backed salamander, Plethodon cinereus, is an important species in the study of these chemical defenses. During the day and early evening, this species avoids rinses from garter snakes, Thamnophis sirtalis, independent of snake diet, but late at night, avoids only those rinses from garter snakes that have recently eaten P. cinereus. We tested whether the selective, late-night response requires the ingestion or injury of salamanders. In three experiments, we tested P. cinereus for their responses to separate or combined rinses from salamanders (undisturbed, distressed, …

Sex Differences And Seasonal Trade-Offs In Response To Injured And Non-Injured Conspecifics In Red-Spotted Newts, Notophthalmus Viridescens, Jason R. Rohr, Dale M. Madison, Aaron M. Sullivan

Faculty Publications - Department of Biological & Molecular Science

Injured prey often release alarm chemicals that induce antipredator behaviors in conspecifics. Injured or killed prey most likely release a wide array of chemicals in addition to alarm substances, such as sexual pheromones, which could enhance or compromise antipredator responses. Thus, damage-release cues provide an excellent opportunity to examine the influence of seasonally fluctuating sexual pheromones on antipredator behaviors. We used a series of laboratory and field experiments and meta-analysis to examine seasonal changes and sex differences in the response of red-spot-ted newts, Notophthalmus viridescens, to the odor of non-injured conspecifics and conspecific tissue extracts, the latter of which presumably …

The Ontogeny Of Chemically-Mediated Antipredator Behaviours In Newts (Notophthalmus Viridescens): Responses To Injured And Non-Injured Conspecifics, Aaron M. Sullivan, Jason R. Rohr, Dale M. Madison

Faculty Publications - Department of Biological & Molecular Science

Responses to alarm chemicals from injured prey may influence predation risk and foraging success of receivers and senders, while learning can influence the strength of these responses. Thus, it is important to know when in ontogeny prey produce and detect alarm substances and how learning shapes their response, but surprisingly little is known about either of these topics. We assessed when in the life of red-spotted newts, Notophthalmus viridescens, alarm chemicals are produced and detected by comparing adult versus eft (terrestrial juveniles) and larval responses to rinses and tissue extracts from individuals in each life-history stage. To evaluate the influence …

The Regulation And Function Of Inositol Phosphorylceramide Synthase In The Yeast Saccharomyces Cerevisiae, Angeleah Arlene Browdy

Open Access Dissertations

IPC Synthase has been shown to be an essential membrane bound enzyme in yeast that catalyzes the transfer of PI to ceramide to yield the primary sphingolipid found in yeast, Inositolphosphorylceramide. A biochemical approach was taken to investigate the biological factors that regulate IC synthase activity in order to better understand the sphingolipid synthesis. A highly purified IPC synthase was purified over 1000-fold from the yeast Saccharomyces cerevisiae by a procedure that included Triton X-100 solubilization, as well as a series of ion exchange and hydrophobic column chromatography steps. The highly purified enzyme fraction contained three major bands at 45, …

Functional Characterization Of The Pregnane X Receptor, He Zhang

Open Access Dissertations

Cytochrome P450 3A enzymes (CYP3A) are involved in the metabolism of two thirds of drugs and other xenobiotics, and highly induced by structurally diverse xenobiotics. Induction of CYP3A causes many drug-drug interactions, and an orphan nuclear receptor, the pregnane X receptor (PXR) is established to mediate the induction of CYP3A in mouse and human. Structurally, the PXR has a N-terminal uncharacterized domain, a conserved DNA binding domain (DBD) and a diverse C-terminal ligand binding domain (LBD). The purpose of the studies described in this dissertation is to characterize the role of the N-terminal domain and the LBD in PXR-mediated signaling …

The Egyptian Pharmaceutical Industry After Trips — A Practitioner's View, Nermien Al-Ali

Fordham International Law Journal

The pharmaceutical industry in Egypt is the largest in the Middle East and North Africa (“MENA”) region, and one that has attracted foreign investment despite the fact that Egyptian law has not always provided pharmaceuticals with patent protection. The situation changed with the signing of the Agreement on Trade-Related Aspects of Intellectual Property Rights (“TRIPS agreement” or “TRIPS”), strengthening the interest of the U.S. and other pharmaceutical companies in increasing their investment in Egypt. The Egyptian pharmaceutical industry is currently undergoing many changes spurred by the implementation of TRIPS and the globalization of trade. Many of these changes can be …

Sentencing Guidelines For Copyright Pirates In The United States And The Hong Kong Special Administrative Region: A Comparative Perspective, Jonathan J. Rusch

Fordham International Law Journal

As more and more nations prosecute copyright piracy cases, it is far from clear whether these nations, in seeking to protect legitimate copyright interests, will also recognize the need to achieve three goals in the sentencing of such cases. The first is honesty in sentencing: that is, avoiding situations in which the nominal sentence that a court initially imposes at sentencing may later be substantially reduced through the parole process. The second is reasonable uniformity in sentencing, so that courts do not have wide disparities in the sentences they impose on similar offenders who commit similar criminal offenses. The third …

Pterodactyl Wings, Greg J. Hobbs

Water Law Review

No abstract provided.

To A Friend Who Would Be Secretary Of Interior, Greg J. Hobbs

Water Law Review

No abstract provided.

Yesterday The Denver Post Reported, Greg J. Hobbs

Water Law Review

No abstract provided.

Wisconsin V. Epa: Tribal Empowerment And State Powerlessness Under 518(E) Of The Clean Water Act, Paul M. Drucker

Water Law Review

No abstract provided.

Gaudreau V. Clinton Irrigation Dist., 30 P.3d 1070 (Mont. 2001), Matthew J. Costinett

Water Law Review

No abstract provided.

In Re Deadman's Basin Water Users Ass'n, 40 P.3d 387 (Mont. 2002), Melissa L. Gordon

Water Law Review

No abstract provided.

Vol. 5, No. 2: Full Issue, Water Law Review

Water Law Review

No abstract provided.

Carter's Groundbreaking Appointment Of Women To The Federal Branch: His Other Human Rights Record, Mary Clark

Scholarly Articles in Law Reviews & Journals

No abstract provided.