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2000

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Creation And Characterization Of 25 Kda Outer Membrane Protein (Omp25) Deletion Mutants In Brucella Species., Matthew David Edmonds Jan 2000

Creation And Characterization Of 25 Kda Outer Membrane Protein (Omp25) Deletion Mutants In Brucella Species., Matthew David Edmonds

LSU Historical Dissertations and Theses

The genus Brucella is composed of Gram-negative, facultative intracellular pathogens that impact man and animals. Brucella species express a 25 kDa major outer membrane protein termed Omp25. To determine the role of Omp25 in virulence, mutants were created in Brucella abortus (BA25), Brucella melitensis (BM25), and Brucella ovis (BO25) that contain disruptions in the omp25 gene (Deltaomp25 mutants). In the murine brucellosis model, each Deltaomp25 mutant was attenuated when compared to the virulent parental strain. To assess the ability of the B. abortus and B. melitensis mutants to colonize and induce abortions in the ruminant host, cattle and goats in …

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Comparing Interventions On Disruptive Classroom Behaviors Derived From Descriptive Analysis, Experimental Analysis, And Reinforcer Assessment., Heather Peshak George Jan 2000

Comparing Interventions On Disruptive Classroom Behaviors Derived From Descriptive Analysis, Experimental Analysis, And Reinforcer Assessment., Heather Peshak George

LSU Historical Dissertations and Theses

This study investigated whether descriptive analyses, experimental analyses, and reinforcer assessments produced similar patterns of data. Descriptive analyses were conducted to identify baseline levels of disruptive behavior and to systematically describe the co-variation between disruptive behaviors and teacher attention, peer attention, and the academic task. Data on disruptive behavior were analyzed by computing conditional probabilities associated with the preceding and maintaining stimuli (e.g., peer attention). Experimental analyses were conducted to examine the extent to which disruptive behavior was sensitive to the instructional task or to the systematically programmed consequences (e.g., teacher attention). A preference assessment was conducted to identify preferred …

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Studies On The Adsorption Of Di- And Mono-Isocyanides Onto Gold And Plantinum Surfaces., Song Lin Jan 2000

Studies On The Adsorption Of Di- And Mono-Isocyanides Onto Gold And Plantinum Surfaces., Song Lin

LSU Historical Dissertations and Theses

The successful formation and characterization of 1,6-diisocyanohexane (DICH) molecules self assembled onto Au and Pt is realized in this dissertation. Exposure of Au substrates to methanolic solutions of DICH can result in the formation of either a monolayer or polymeric multilayer of DICH. Cyclic voltammograms of solution-phase redox probes at poly(DICH)-coated electrode surfaces (both Au and Pt) exhibit behavior that demonstrates that the poly(DICH) layers are effective transport barriers. Cross-linking of the poly(DICH) films can be increased by treating the poly(DICH) films with aqueous Ni2+ solutions; in addition, the free isocyanide functionalities in the poly(DICH) films can be electrochemically converted …

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Development Of Miniaturized Devices Using X -Ray Microlithography In Poly(Methyl Methacrylate) For Chemical Monitoring And Microfluidic Applications., Christopher Scott Mcwhorter Jan 2000

Development Of Miniaturized Devices Using X -Ray Microlithography In Poly(Methyl Methacrylate) For Chemical Monitoring And Microfluidic Applications., Christopher Scott Mcwhorter

LSU Historical Dissertations and Theses

The research presented in this document is focused on the development and characterization of novel microfluidic devices using X-ray photolithography in poly(methyl methacrylate) (PMMA). A conductivity detector cell was developed and characterized as a sensitive detector for DNA and KCl using flow injection, then was integrated into a high-performance liquid chromatography system for the purification of PCR amplicons. A planar conductivity cell was then developed on a microchip and its detection sensitivity was characterized by pressure pumping KCl through the detector and monitoring the change in conductivity. Also, the topography of the device was characterized using scanning electron and optical …

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Professional Versus Caregiver Ratings Of The Communicative Abilities Of Adults Displaying Moderate To Profound Communication Challenges., Paul Steven Miller Jan 2000

Professional Versus Caregiver Ratings Of The Communicative Abilities Of Adults Displaying Moderate To Profound Communication Challenges., Paul Steven Miller

LSU Historical Dissertations and Theses

Ninety caregivers of 30 adults with moderate-to-serve cognitive, sensory, and physical challenges living in an institutional setting participated in this study to investigate caregiver perceptions of communicative abilities. A mixed effects design was used to compare communication scores of thirty adults. The adults were assigned to three communication proficiency groups. The communicative scores with challenges that were generated with three different assessment activities: a direct/participatory evaluation conducted by a certified speech-language pathologist, an interview of the caregivers, and an observation of daily routine activities. Results indicated that all of the rating procedures differentiated the three communication proficiency groups. Correlations between …

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Evaluating Productivity, Nesting Success, And Conservation Of Thick -Billed Parrots., Tiberio Cesar Monterrubio Rico Jan 2000

Evaluating Productivity, Nesting Success, And Conservation Of Thick -Billed Parrots., Tiberio Cesar Monterrubio Rico

LSU Historical Dissertations and Theses

I studied the breeding activity, productivity and nesting success of the Thick-billed Parrot (Rhynchopsitta pachyrhyncha) during a 3-year period 1997--1999, and also analyzed preliminary data from 1995 and 1996. I studied the Parrots in six nesting areas located in the Sierra Madre Occidental in Mexico. Data were collected on breeding areas characteristics, nest site characteristics, reproductive activity, productivity, and nesting success. Most of the historical breeding range reported in the literature was searched for nests. Two main breeding areas reported in the literature continue to be the major breeding strongholds for the species. Cebadillas and Madera accounted for 56% and …

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Synthesis And Characterization Of Alkoxyaniline-Terminated Monomer Monolayers On Gold Surfaces., Keith Cory Schomburg Jan 2000

Synthesis And Characterization Of Alkoxyaniline-Terminated Monomer Monolayers On Gold Surfaces., Keith Cory Schomburg

LSU Historical Dissertations and Theses

Monolayers of 2-(6-mercaptohexan-1-oxy)aniline (AnC6SH) and 2-(7-mercaptoheptan-1-oxy)aniline (AnC7SH) on Au were synthesized and investigated by reflection-absorption infrared (RAIR) spectroscopy and electrochemistry. Electrochemical oxidation of AnC6SH/Au and AnC7SH/Au in 1 M H2SO4 to form ox-AnC6SH/Au and ox-AnC7SH/Au resulted in voltammetry indicative of oligomerization and degradation reactions of the aniline functionality. Scanning of the voltammetric potential between 0.0 and +0.9 V and then back to 0.0 V vs. SSCE twice leads to four surface-confined redox waves occurring at +0.25, +0.45, +0.5, and +0.6 V vs. SSCE. The oxidized monolayers were found to exhibit voltammetry similar to that observed for poly(o-phenetidine), which exhibits redox …

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Theoretical And Experimental Studies Of Flame -Generated Agglomerates Using Light Scattering., Guocai Shu Jan 2000

Theoretical And Experimental Studies Of Flame -Generated Agglomerates Using Light Scattering., Guocai Shu

LSU Historical Dissertations and Theses

Knowledge of optical properties and morphological characteristics of aggregated aerosols is important for pollution control, material synthesis through the aerosol route, oxidation of particulate matters, as well as understanding the mechanism of particulate formation and growth. However, the limitations of current light scattering theories as well as the lack of robust data inversion schemes render the complete characterization of the agglomerated structures difficult and time consuming. The objective of present work is to develop robust experimental and theoretical methods to characterize the aggregated aerosols from in-situ classical light scattering (CLS) as well as dynamic light scattering (DLS) measurements. In the …

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Insecticide Resistance And Resistance Mechanisms In The Southern House Mosquito, Culex Quinquefasciatus., Jeffrey Dwayne Stancil Jan 2000

Insecticide Resistance And Resistance Mechanisms In The Southern House Mosquito, Culex Quinquefasciatus., Jeffrey Dwayne Stancil

LSU Historical Dissertations and Theses

Significant resistance to malathion and resmethrin was found in insecticide-treated field populations of adult Culex quinquefasciatus , Say, compared with a laboratory and non-treated field strain. Resistance to both insecticides varied during the active control season and was associated with the frequency of insecticide application. Geographic variations in resistance levels were noted, and significant differences occurred between separate populations within the same mosquito control districts. Four model substrates were assessed for their suitability in detecting elevated esterase activity associated with malathion resistance. Esterase activities towards all substrates were significantly higher in field stains than a laboratory strain. Esterase activities toward …

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Comparative Efficacy And Quality Characterizatin Of Mayhaw (Crataegus Opaca) Juice Extraction., Alfred Francois Trappey Ii Jan 2000

Comparative Efficacy And Quality Characterizatin Of Mayhaw (Crataegus Opaca) Juice Extraction., Alfred Francois Trappey Ii

LSU Historical Dissertations and Theses

The comparative efficacy and quality characterization of juice by six extraction methods utilizing either frozen or fresh 'Texas Star' mayhaw (Crataegus opaca Hook. and Arn.) fruit obtained from the 1998 and 1999 crop season were conducted according to temperature (cold-press or hot-press extraction) and fruit pulp pretreatment (whole or pulverized fruit with or without pectolytic enzyme). Extraction methods were steam extraction using whole fruit without added pulp juice (SE); steam extraction using whole fruit with added pulp juice (SEP); cold-press extraction using whole fruit (CPE); cold-press extraction using finely macerated fruit pulp (CPEG); hot-press extraction using finely macerated fruit pulp …

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Consumers' Reasoned Behavior Toward American Alligator Leather Products., Yingjiao Xu Jan 2000

Consumers' Reasoned Behavior Toward American Alligator Leather Products., Yingjiao Xu

LSU Historical Dissertations and Theses

This study determined consumers' perception of and attitude toward American alligator leather products and evaluated factors affecting consumers' purchase intention of the products. The Fishbein and Ajzen (1975, 1980) theory of reasoned behavior served as the theoretical framework for the study. The influence of six variables on purchase intention was measured: attitude toward purchase based on product attributes, subjective norm with respect to purchase, traditional attitude toward fashion products (fashion involvement), personality traits (self-confidence and self-consciousness), price perception (price-quality schema and prestige sensitivity), and demographics. The correlation between consumers' usage of media for fashion information and purchase intention was also …

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Terrebonne Farms, Louisiana: An Anthropogeographic Study Of A New Deal Resettlement., Lisa Kay Adam Jan 2000

Terrebonne Farms, Louisiana: An Anthropogeographic Study Of A New Deal Resettlement., Lisa Kay Adam

LSU Historical Dissertations and Theses

This dissertation examines Terrebonne Farms, a 1940's community in south Louisiana established by the federal government during the New Deal period. Planned by the Resettlement Administration and overseen by the Farm Security Administration, the community was one of approximately one hundred experimental towns or resettlements located around the nation. Although these resettlements varied widely in their organization, appearance, and history, few writers have chosen to focus on individual communities. This study combines recollections of original residents and local accounts of Terrebonne Farms with its government records and photographs to create a detailed and contextual description of a single resettlement. In …

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Litterfall Dynamics And Nutrient Cycling In Mangrove Forests Of Southern Everglades, Florida And Terminos Lagoon, Mexico., Carlos Antonio Coronado Molina Jan 2000

Litterfall Dynamics And Nutrient Cycling In Mangrove Forests Of Southern Everglades, Florida And Terminos Lagoon, Mexico., Carlos Antonio Coronado Molina

LSU Historical Dissertations and Theses

The objective of this dissertation was to investigate the processes that control litterfall and nutrient dynamics in two contrasting mangrove ecosystems at the Southeastern (SE) Everglades and Terminos Lagoon. The main objectives were: (a) to determine the spatial and temporal variability of litterfall dynamics, (b) to evaluate the relative importance of nutrient use efficiency as a nutrient conservation mechanism along a natural nutrient gradient, and (c) to assess the relative importance of nutrient resorption on litterfall dynamics. Two hypotheses were tested: (a) mangrove species living in nutrient-poor environments and in nutrient-rich environments do not differ in the efficiency in using …

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Chiral Recognition Using Polymeric And Monomeric Amino Acid Based Surfactants., Fereshteh Haddadian Billiot Jan 2000

Chiral Recognition Using Polymeric And Monomeric Amino Acid Based Surfactants., Fereshteh Haddadian Billiot

LSU Historical Dissertations and Theses

The research presented in this dissertation involves the comparison and characterization of polymeric and monomeric amino acid based surfactants for the enantiomeric separation of chiral compounds. Comparison of the performance of these two kinds of surfactants in EKC suggest that polymers are better chiral pseudostationary phases (CPSP) than the monomers for enantiomeric separation of neutral and cationic analytes, at least for the examined in this dissertation. However, the anionic enantiomers of 1,1'-binaphthyl-2-2 '-dihydrogen phosphate (BNP) were better separated using monomeric CPSPs. The depth of penetration of binaphthyl derivatives into the micellar core of chiral dipeptide surfactants (CDS) was examined using …

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Influence Of Pyrithiobac And Insecticide Combinations On Cotton Growth And Early Season Pest Control., Richard Whitman Costello Jan 2000

Influence Of Pyrithiobac And Insecticide Combinations On Cotton Growth And Early Season Pest Control., Richard Whitman Costello

LSU Historical Dissertations and Theses

Field studies were conducted at two locations in Northeast Louisiana, to investigate interactions associated with the herbicide pyrithiobac and insecticide combinations in respect to weed control and insecticide efficacy and cotton response. In addition, controlled environment experiments were conducted to evaluate the effect of temperature regimes and simulated thrips damage on pyrithiobac plus insecticide phytotoxicity to cotton. Pyrithiobac and insecticide combinations when compared with pyrithiobac alone did not reduce cotton leaf area, height, main stem node number, main stem nodes to first square, days to first square or flower, main stem nodes above white flower, or seedcotton yield. Acephate insecticide …

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Female High School Biology Students' Biofilm -Focused Learning: The Contributions Of Three Instructional Strategies To Patterns In Understanding And Motivation., Jo Dale Hill Ales Jan 2000

Female High School Biology Students' Biofilm -Focused Learning: The Contributions Of Three Instructional Strategies To Patterns In Understanding And Motivation., Jo Dale Hill Ales

LSU Historical Dissertations and Theses

This exploratory study examined three instructional strategies used with female high school biology students. The relative contributions of the strategies to student understanding of microbiology and motivation in science were analyzed. The science education community targeted underachievement in science by implementing changes in content and practices (NRC, 1996). Research suggested that teachers facilitate learnirig environments based on human constructivism (Mintzes, Wandersee, & Novak, 1997) that is rooted in meaningful learning theory (Ausubel, Novak & Hanesian, 1978). Teachers were advised to use both visual and verbal instructional strategies (Paivio, 1983) and encourage students to construct understandings by connecting new experiences to …

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Component Reliability Estimation From Partially Masked And Censored System Life Data Under Competing Risks., Rudi Hendricus Petrus Martinus Arts Jan 2000

Component Reliability Estimation From Partially Masked And Censored System Life Data Under Competing Risks., Rudi Hendricus Petrus Martinus Arts

LSU Historical Dissertations and Theses

This research presents new approaches to the estimation of component reliability distribution parameters from partially masked and/or censored system life data. Such data are common in continuous production environments. The methods were tested on Monte Carlo simulated data and compared to the only alternative suggested in literature. This alternative did not converge on many masked datasets. The new methods produce accurate parameter estimates, particularly at low masking levels. They show little bias. One method ignores masked data and treats them as censored observations. It works well if at least 2 known-cause failures of each component type have been observed and …

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Removal Of Manganese In Reservoir Tailwaters As A Function Of Reservoir Operations And River Substrate., Steven Lane Ashby Jan 2000

Removal Of Manganese In Reservoir Tailwaters As A Function Of Reservoir Operations And River Substrate., Steven Lane Ashby

LSU Historical Dissertations and Theses

Removal of reduced manganese in aqueous systems has been described as a two-step process that incorporates a chemical or abiotic step followed by or continued with microbially mediated processes. Field and laboratory studies were conducted to describe the removal of manganese from reservoir tailwaters and partition the abiotic and microbial removal processes. Manganese removal was measured in controlled raceways and in microcosms with four sizes of substrate (cobble, 2.00 to 0.50 mm, 0.50 mm to 0.063 mm, and less than 0.063 mm). Sodium azide was used to inhibit biological uptake. An exponential decay model and a hyperbolic decay model were …

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The Rhetoric Of The Benign Scapegoat: President Reagan And The Federal Government., Stephen Wayne Braden Jan 2000

The Rhetoric Of The Benign Scapegoat: President Reagan And The Federal Government., Stephen Wayne Braden

LSU Historical Dissertations and Theses

Ronald Reagan deservedly attained the moniker "The Great Communicator" due to his rhetorical prowess. This dissertation addresses an aspect, governmental scapegoating, of Reagan's rhetorical strategy. Traditional scapegoating theory claims that the scapegoat is either killed or driven from the community. However, I claim that Reagan profited by making a unique use of the scapegoat, one that was benign, the government remained essentially unscathed. The means to analyze Reagan's discourse is Kenneth Burke's cycle of guilt, victimage, redemption and rebirth. Ten speeches of President Reagan are analyzed: both of his Inaugural Addresses, his Economic Recovery Speech, and his seven State of …

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Intention To Leave Teaching: A Study Of Louisiana Elementary School Teachers Early In Their Careers., Karen Elizabeth Callender Jan 2000

Intention To Leave Teaching: A Study Of Louisiana Elementary School Teachers Early In Their Careers., Karen Elizabeth Callender

LSU Historical Dissertations and Theses

The purpose of this study was to determine the relationship between the intention to leave teaching of teachers early in their careers and the variables: teacher self-efficacy, teacher age, school size, community type, socioeconomic background of student population, student discipline, and salary. Both quantitative and qualitative methods were employed to investigate these relationships. Survey data were collected from kindergarten through fifth grade teachers in Louisiana public schools, and interviews were conducted with those reporting the highest intention to leave. The quantitative data revealed school size to be significantly related to teacher intention to leave teaching, and teacher self-efficacy, student SES, …

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Heritability Of Waterlogging Tolerance In Wheat., Agron Collaku Jan 2000

Heritability Of Waterlogging Tolerance In Wheat., Agron Collaku

LSU Historical Dissertations and Theses

Waterlogging stress is one of the factors depressing wheat yield. Study of heritability for yield and yield components under waterlogging is important in determining the influence of genetic factors accounting for waterlogging tolerance as compared to environmental stress. The objectives of this study were: (1) to study the effect of waterlogging on yield and yield components of soft red winter wheat; (2) to estimate losses from waterlogging; (3) to estimate heritability of waterlogging tolerance in early generations of wheat for yield and yield components, and (4) to provide selection criteria for waterlogging tolerance in wheat breeding. Linear reduction in yield …

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Modified Atmosphere Packaging Of Fresh -Cut Sweetpotatoes., Elif Erturk Jan 2000

Modified Atmosphere Packaging Of Fresh -Cut Sweetpotatoes., Elif Erturk

LSU Historical Dissertations and Theses

Fresh-cut sweetpotato products have potential to create new value-added market opportunities. To determine optimum film packaging and storage temperature, fresh-cut sweetpotato slices were packaged with semipermeable polyolefin films, having different gas transmission characteristics and kept at 2 or 8°C for 14 days. The films chosen, in order of increasing permeability, were Cryovac PD900, PD961, and PD941. Among the films, only PD941 film bags maintained an aerobic atmosphere during the 14 days at both temperatures. However, the high weight loss in slices with PD941 film bags will limit the use of these film bags as modified atmosphere packaging (MAP) material. PD961 …

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The Viola Compositions Of Ernst Mahle And Their Idiomatic And Pedagogical Characteristics., Sonia Feres-Lloyd Jan 2000

The Viola Compositions Of Ernst Mahle And Their Idiomatic And Pedagogical Characteristics., Sonia Feres-Lloyd

LSU Historical Dissertations and Theses

Ernst Mahle was born in Germany, in 1929, but has lived in Brazil since 1951, where he is one of the most important composers of his generation. Besides his work as a composer, he has been developing a very important pedagogical work in Piracicaba, a city in the state of Sao Paulo, Brazil. Influenced by the teachings of Hans Joachim Koellreutter, Mahle, together with other influential art supporters from that city, founded the Escola de Musica de Piracicaba, in 1953. Mahle believes that it is very important to teach music when children are young. He also encourages practice in musical …

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An Inquiry Into The Impact Of Teacher Variances Within The Implementation Of The Success For All Reading Program In Two School Districts., Karen Parker Guillot Jan 2000

An Inquiry Into The Impact Of Teacher Variances Within The Implementation Of The Success For All Reading Program In Two School Districts., Karen Parker Guillot

LSU Historical Dissertations and Theses

Elementary school students walk single file to their respective classrooms where literacy should miraculously transpire. For literacy to occur, some schools adopt reading programs provided by their local district or state, while others utilize externally developed program designs. This multiple-case qualitative inquiry examined the externally developed program, Success for All, a program designed to benefit high poverty schools, founded by Robert Slavin and Nancy Madden as well as their cohorts at Johns Hopkins University. This research study examined the variances among seven teachers implementing the SFA reading program, "Reading Wings," in three schools in two school districts in Louisiana. It …

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Development Of Novel Separation Strategies: Part I. The Separation And Characterization Of The Insecticidal Pyrethrin Esters. Part Ii. Separations In Capillary Electrochromatography., Charles William Henry Iii Jan 2000

Development Of Novel Separation Strategies: Part I. The Separation And Characterization Of The Insecticidal Pyrethrin Esters. Part Ii. Separations In Capillary Electrochromatography., Charles William Henry Iii

LSU Historical Dissertations and Theses

The analytical investigation of the natural insecticide pyrethrum has been conducted. In this research, novel approaches were designed to yield either faster, or more complete separation of the active pesticidal esters in pyrethrum extracts as well as more complete conformational information of the structures of these compounds in solution. A combination of techniques was used: micellar electrokinetic chromatography (MEKC), nuclear magnetic resonance spectroscopy (NMR), and capillary electrochromatography (CEC). Using MEKC, an optimized separation of the six pyrethrin esters was achieved in 25 minutes with 25 mM Tris, buffered at pH 9, containing 30 mM SDS, 25% (v/v) acetonitrile, and an …

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The Effects Of The Laboratory On College Students' Understanding Of Evolution: Implications For Conceptual Change., Lorna Benita Holtman Jan 2000

The Effects Of The Laboratory On College Students' Understanding Of Evolution: Implications For Conceptual Change., Lorna Benita Holtman

LSU Historical Dissertations and Theses

This study investigated eighty junior and senior college students' understanding of evolutionary biology concepts in lecture-only and lecture-laboratory settings. The evolution lab stressed the processes of evolution, and involved simulations, experiments, discussions, report writing, and reading. Test scores do not reveal everything about the actual process of learning in the laboratory. This study examined conceptual change patterns over a period of one semester using in-depth interviews with eight participants. The study revealed that the lecture-laboratory group performed better than the lecture-only group on certain shared items on the objective examination. The interview participants showed various patterns of conceptual change; that …

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The Welfare And Distribution Impacts Of International Agricultural Trade Policies: An Analysis Of Turkey's Integration Into The European Union., Cemal Atici Jan 2000

The Welfare And Distribution Impacts Of International Agricultural Trade Policies: An Analysis Of Turkey's Integration Into The European Union., Cemal Atici

LSU Historical Dissertations and Theses

The objective of this dissertation is to examine the agricultural trade policies of Turkey and determine the impact of various relevant policy scenarios on the welfare and distribution of income within the agricultural sector as well as between producers and consumer groups. The main goal of this research is to quantify the impact of trade liberalization resulting from multilateral agricultural trade agreements and the formation of regional trading blocs on Turkish agriculture and, consequently, the income distribution effects in the various producer and consumer groups. The study utilizes a partial equilibrium framework Modele Internationale Simplifie de Simulation (MISS) to analyze …

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The Function Of Speech (Verbal Mediation) On Corresponding Academic And Social Behavior., Kimberly Jo Callicott Jan 2000

The Function Of Speech (Verbal Mediation) On Corresponding Academic And Social Behavior., Kimberly Jo Callicott

LSU Historical Dissertations and Theses

The role of language in self-control has been of interest to researchers across continents and time. This study attempted to isolate the functional role of language in relationship to subsequent academic and task engaged behaviors with four students aged 10--14. The students were diagnosed with Emotional or Behavioral Disorders had normal intelligence and were receiving their education at an alternative placement facility. The study used a single subject design and exposed the subjects to conditions of self-declaratory speech (SDS), SDS and reinforcement for correspondence, a return to baseline, SDS and reinforcement for correspondence, and SDS with delayed reinforcement. Results of …

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Synthesis Of Phosphonate And Thiophosphonate Peptide Analogs As Inhibitors Of Carboxypeptidase A., Hong Fan Jan 2000

Synthesis Of Phosphonate And Thiophosphonate Peptide Analogs As Inhibitors Of Carboxypeptidase A., Hong Fan

LSU Historical Dissertations and Theses

Phosphonate analogs of peptides are important in biochemical research and potentially as medicinal agents because of their ability to strongly inhibit both metallo and aspartyl proteases. Although a number of phosphorus-containing molecules were reported as inhibitors for carboxypeptidase A (CPA, there have been only a few thiophosphonopeptide inhibitors reported. There has been no investigation in which the Ki values of a series of thiophosphonate inhibitors was compared to the analogous phosphonates to probe the role the sulfur atom in inhibition. The goal of this work is to prepare phosphonopeptide inhibitors of CPA and their thio-analogs so that this comparison can …

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Immobilization Of Enzymes On Porous Silica For The Micro-Digestion Of Oligonucleotides With Analysis By Ce., Yolanda Yvette Davidson Jan 2000

Immobilization Of Enzymes On Porous Silica For The Micro-Digestion Of Oligonucleotides With Analysis By Ce., Yolanda Yvette Davidson

LSU Historical Dissertations and Theses

The research in this dissertation has focused on the immobilization of enzymes on silica to perform digestions with DNA and RNA with subsequent analysis by capillary electrophoresis. Solid-phase DNA restriction digest reactors were developed consisting of silica particles modified with a covalently tethered restriction enzyme. This solid-phase restriction reactor enables digestion and separation of minute quantities of DNA with minimal reagent consumption. The restriction enzymes, HaeIII, PstI and HindIII, were successfully immobilized via glutaraldehyde linkages to porous silica micro-particles. Studies were done to examine the impact of immobilization on enzymatic activity. Digestions of &phis;X174-RF DNA phage and SV40 Viral DNA …

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