Digital Commons Network^™

Annual Report Of The Town Of Tilton, New Hampshire For The Year Ending December 31, 1999., Tilton Town Representatives

Tilton, NH Annual Reports

This is an annual report containing vital statistics for a town/city in the state of New Hampshire.

State Of New Hampshire Annual Report Of Coos County For The Year Ending December 31, 1999., Coos County Representatives

Coos County, NH Annual Reports

This is an annual report containing vital statistics for a county in the state of New Hampshire.

Toward A Formative Project Of Securing Freedom And Equality , Linda C. Mcclain

Cornell Law Review

No abstract provided.

Ec00-1207 Growing Onions, Shallots, And Chives, Susan Schoneweis, Laurie Hodges, Loren J. Giesler

University of Nebraska-Lincoln Extension: Historical Materials

The common onion (Allium cepa) is the most popular and widely grown Allium in Nebraska home gardens. Many members of the onion family (Alliaceae) are used in flower gardens because of their interesting flowers and foliage, but here we're going to focus on the onion as a vegetable crop.

This publication will discuss the different types of onions and how to grow onions from onion sets, plants, transplants, and seed. It will cover the different cultivars found here in Nebraska, the nutrition, fertilizing, harvesting, curing and storing of onions.

Season And Distance From Forest - Old Field Edge Affect Seed Predation By White-Footed Mice, Scott J. Meiners, James T. Mccormick

Faculty Research & Creative Activity

We studied the spatial pattern of seed predation across a forest-old field edge in both fall and winter to assess the potential for seed predators to influence plant spatial patterns. We used a 100 x 100 m grid that began 30 m inside the forest and extended 60 m into the old field. Inside this grid we placed seed stations at regular 10 m intervals and monitored seed removal. Seed predation varied significantly across the edge gradient in both fall and winter with the highest rate of seed removal at the edge in both trials. The spatial pattern of seed …

Metals And Metallothionein In The Liver Of Raccoons: Utility For Environmental Assessment And Monitoring, Joanna Burger, Christine G. Lord, Edward J. Yurkow, Lynn Mcgrath, Karen F. Gaines, I. Lehr Brisbin Jr., Michael Gochfeld

Faculty Research & Creative Activity

The relationship between metallothionein levels and concentrations of several metals and radionuclides was examined in liver tissues of raccoons (Procyon lotor, n = 47) from the Department of Energy’s Savannah River Site in South Carolina to determine the applicability of metallothioneins as an initial screening device for exposure assessment in free-living mammals and environmental monitoring. Using a fluorescent marker and a cell sorter to measure metallothionein, a significant positive correlation was found across animals between levels of metallothioneins and concentrations of selenium (Pearson’s r = .30) , mercury (Pearson’s r = .31) , and copper (Pearson’s r = .30) in …

Proximate Constraints On Intruder Detection In The Dragonfly Perithemis Tenera (Odonata: Libellulidae): Effects Of Angle Of Approach And Background, Paul V. Switzer, Perri K. Eason

Faculty Research & Creative Activity

The implications of insects’ vision for territorial defense have been relatively little studied in the field. In the dragonfly Perithemis tenera Say we investigated whether either the angle at which an intruder was viewed by a territorial resident or the background against which it was viewed affected the detection of that intruder. Residents detected intruders at a greater distance if the intruders were directly in front of them; they also detected more intruders in front of them than from other angles. Intruders viewed against distant vegetation were detected more readily than were intruders against near vegetation. Residents detected more intruders …

Faith, Hope, And Rationality: Or, Public Choice And The Perils Of Occam's Razor, Cynthia R. Farina

Florida State University Law Review

No abstract provided.

Genetic Diversity And Transcriptional Analysis Of The Bys1 Gene From Blastomyces Dermatitidis, James L. Bono, Basam Jaber, Mark A. Fisher, Raed O. Abuodeh, Erin O’Leary-Jepson, Gene M. Scalarone, Laurens H. Smith Jr.

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Blastomyces dermatitidis, a pathogenic fungal organism, is able to exist in two differentmorphologies, a multicellular mycelium or a unicellular yeast, according to temperature, 25˚C and 37˚C respectively. The switching between morphologies must be accompanied by a cascade of signaling events in which expression of genes responsible for the change of morphology is increased or decreased. bys1, a gene from B. dermatitidis isolate #58, is expressed at high levels in the unicellular yeast, but gradually diminishes as the temperature is lowered and the organism converts to the mycelial phase where there is no transcription of bys1. We explored …

Effect Of Antimicrobial Agents On Livestock Waste Emissions, Vincent H. Varel, Daniel N. Miller

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Various antimicrobial agents were evaluated with the purpose of reducing the microbial fermentation in stored cattle waste and the resulting odor emissions. Duplicate sealed 2-L flasks with 500 ml waste slurry, with and without antimicrobial inhibitors, were used to measure the production of short-chain volatile fatty acids, lactate, and total fermentation gas over 27–30 days. A combination of chlorhexidine diacetate (2 mM), iodoacetate (2 mM), and α-pinene (3.8 mM) reduced gas production 80% (1000 ml to 200 ml) and total volatile fatty acid production 50% (145 mM to 72 mM). Pinene had little antimicrobial effect; rather, it served as an …

Mapping Quantitative Trait Loci For Bovine Ovulation Rate, Brian W. Kirkpatrick, Becky M. Byla, Keith E. Gregory

Roman L. Hruska U.S. Meat Animal Research Center: Reports

An elite, three-generation family from the USDA Meat Animal Research Center twinning population was examined for evidence of ovulation rate quantitative trait loci (QTL). This work was both a continuation of previously reported results suggesting evidence for ovulation rate QTL on bovine Chromosome (Chr) 7 and an extension of a genome-wide search for QTL. Additional markers were typed on Chr 7 to facilitate interval mapping and testing of the hypothesis of one versus two QTL on that chromosome. In addition, 14 other informative markers were added to a selective genotyping genome screening of this family, and markers exhibiting nominal significance …

Comparative Map Alignment Of Bta27 And Hsa4 And 8 To Identify Conserved Segments Of Genome Containing Fat Deposition Qtl, Tad S. Sonstegard, Wes M. Garrett, Melissa S. Ashwell, Gary L. Bennett, Steven M. Kappes, Curtis P. Van Tassell

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Quantitative trait loci (QTL) associated with fat deposition have been identified on bovine Chromosome 27 (BTA27) in two different cattle populations. To generate more informative markers for verification and refinement of these QTL-containing intervals, we initiated construction of a BTA27 comparative map. Fourteen genes were selected for mapping based on previously identified regions of conservation between the cattle and human genomes. Markers were developed from the bovine orthologs of genes found on human Chromosomes 1 (HSA1), 4, 8, and 14. Twelve genes were mapped on the bovine linkage map by using markers associated with single nucleotide polymorphisms or microsatellites. Seven …

Development And Demonstration Of Rna Isolation And Rt-Pcr Procedures To Detect Escherichia Coli O157:H7 Gene Expression On Beef Carcass Surfaces, E. D. Berry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Preventing the development of pathogen resistance to processing and preservation techniques will require an understanding of the genetic mechanisms that pathogens use in situ to adapt and develop tolerance to stresses they encounter in the food environment. RNA isolation and reverse-transcription (RT)-PCR protocols were developed as tools to detect gene expression in bacteria on beef carcass surfaces. The utility of these procedures was demonstrated by detecting the expression of a selectively-inducible green fluorescent protein (GFP) gene in a plasmid-transformed strain of Escherichia coli O157:H7 inoculated onto beef carcass surface tissue. These procedures should serve as useful tools for studying the …

Current Trends And Future Directions In Product Liability In Australia, Jocelyn Kellam, Bettina Arste

William Mitchell Law Review

No abstract provided.

"Vertebrate Pests Of Agriculture, Forestry And Public Lands" 2000 Annual Meeting

Western Region Coordinating Committee for Vertebrate Pests of Agriculture, Forestry, and Public Lands (WCC-95)

Table of Contents ........................................................... i

2000 Officers ............................................................... 1

Minutes ................................................................... 2

Number and Title of the Regional Project ................................... 2

Location and Dates of the Meeting ....................................... 2

Participants .......................................................... 2

Attendees ............................................................ 3

Adopted Agenda ...................................................... 4

Convening of Sessions (Tuesday 14 November) ............................... 4

Forum: Internet Resources in Wildlife Damage Management .................... 4

2000 Business Meeting (Wednesday 15 November) ............................ 4

Presentations (Wednesday 15 November) .................................... 9

Continued Presentations (Thursday 16 November) ........................... 10

Individual Updates ................................................... 11

Completion of Sessions ................................................ 13

Participants/ Attendees Names and Addresses . . . . . . . . . …

Ld 1028 - Protection Of Indian Archaeological Sites: Report To The Standing Committee On Appropriations And Financial Affairs, Arthur Spiess, Donald Soctomah

Maine Historic Preservation Commission

This report is organized to

1. present the resource and scope of the problem,
2. review the existing laws,
3. discuss the options of site monitoring or stewardship and how they are practiced currently in Maine and elsewhere,
4. present our discussions with law enforcement, and
5. make recommendations to the Legislature.

Porcine Iga Allotypes Are Not Equally Transcribed Or Expressed In Heterozygous Swine, P. Navarro, R.K. Christensen, P. Weber, M. Rothschild, G. Ekhardt, J.E. Butler

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The prediction of 1:1 expression of constant region allotypes in heterozygous animals assumes that productive VDJ rearrangements occur at random between chromosomes, switch recombination is random, there is no allele-related defect in switching and there is no selection for a B-cell receptor bearing a certain constant region allotype. In data reported here, this prediction was often not fulfilled for the transcripts encoding the IgA^a and IgA^b alleles of porcine IgA including those from late term fetal piglets that are not in contact with environmental antigens or maternal regulatory factors. In the spleen, thymus, mesenteric lymph node (MLN), ileal …

Identification And Characterization Of A New Allele For The Beta Subunit Of Follicle-Stimulating Hormone In Chinese Pig Breeds, M. D. Li, G. A. Rohrer, T. H. Wise, J. J. Ford

Roman L. Hruska U.S. Meat Animal Research Center: Reports

During evaluation of follicle-stimulating hormone- beta (FSHB) expression in anterior pituitary glands by an RNase protection assay (RPA), the expected fragment of 205 nucleotides at positions 759-963 was not detected in one boar that had moderate plasma and pituitary FSH concentrations. After subcloning and sequencing, mRNA from this boar lacked an 11-bp fragment (59-CATTTGGAAAC-39) at nucleotide positions 807-817 of the 39-untranslated region (39-UTR, D allele). Wild-type FSHB (WT allele) was present in pituitary RNA and genomic DNA in both Meishan (MS) and White Composite (WC) pigs; whereas the D allele was present only in MS pigs (P < 0·01; 5/6 MS vs. 0/6 WC). Also, we found the D allele in …

Development And Demonstration Of Rna Isolation And Rt−Pcr Procedures To Detect Escherichia Coli O157:H7 Gene Expression On Beef Carcass Surfaces, E. D. Berry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Preventing the development of pathogen resistance to processing and preservation techniques will require an understanding of the genetic mechanisms that pathogens use in situ to adapt and develop tolerance to stresses they encounter in the food environment. RNA isolation and reverse-transcription (RT)−PCR protocols were developed as tools to detect gene expression in bacteria on beef carcass surfaces. The utility of these procedures was demonstrated by detecting the expression of a selectively-inducible green fluorescent protein (GFP) gene in a plasmid-transformed strain of Escherichia coli O157:H7 inoculated onto beef carcass surface tissue. These procedures should serve as useful tools for studying the …

Porcine Erythropoietin Receptor: Molecular Cloning And Expression In Embryonic And Fetal Liver, P. L. Pearson, T. P. L. Smith, T. S. Sonstegard, H. G. Klemcke, R. K. Christenson, J. L. Vallet

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The full coding sequence for porcine erythropoietin receptor (EPOR) was elucidated using reverse transcription polymerase chain reaction (PCR) (rtPCR) and 39 and 59 rapid amplification of cDNA ends (RACE) procedures. Total RNA collected from Day 30 fetal liver was used as starting material. A 1843 bp sequence was obtained from which could be inferred a 509 amino acid protein which was 79–85% identical to the amino acid sequence of erythropoietin receptor from other species. Total RNA samples collected from white crossbred intact, white crossbred UHO and Meishan gilts on Days 24, 30 and 40 of gestation were subjected to Northern …

Mapping Of Seven Bovine Cytokine Genes Involved In T-Lymphocyte Growth, Differentiation And Immune Response, T. S. Sonstegard, W. M. Garrett, G. L. Bennett, S. M. Kappes, D. S. Zarlenga, L. C. Gasbarre

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Source:description

PCR conditions

Polymorphism and allele size

FISH analyses

Chromosomal locations

Mutations In The Genome Of Porcine Reproductive And Respiratory Syndrome Virus Responsible For The Attenuation Phenotype, R. Allende, G. F. Kutish, W. Laegreid, Z. Lu, T. L. Lewis, D. L. Rock, J. Friesen, J. A. Galeota, A. R. Doster, Fernando A. Osorio

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Although live-attenuated vaccines have been used for some time to control clinical symptoms of the porcine reproductive and respiratory syndrome (PRRS), the molecular bases for the attenuated phenotype remain unclear. We had previously determined the genomic sequence of the pathogenic PRRSV 16244B. Limited comparisons of the structural protein coding sequence of an attenuated vaccine strain have shown 98% homology to the pathogenic 16244B. Here we have confirmed the attenuated phenotype and determined the genomic sequence of that attenuated PRRSV vaccine and compared it to its parental VR- 2332 and the 16244B strains. The attenuated vaccine sequence was colinear with that …

Connection, January/February 2000, Eastern Michigan University

Alumni News: Eastern Magazine

No abstract provided.

The Tangled Story Of Kudzu, Kurt E. Kinbacher

Vulcan Historical Review

pp. 45-69

Health, Heart And Mind: The Contributions Of Richard A. Posner To Health Law And Policy, Diane P. Wood

Journal of Contemporary Health Law & Policy (1985-2015)

No abstract provided.

Pesticide Toxicity, Human Subjects, And The Environmental Protection Agency's Dilemma, Heidi Gorovitz Robertson, Samuel Gorovitz

Journal of Contemporary Health Law & Policy (1985-2015)

No abstract provided.

The Defense Threath Reduction Agency: A Note On The United States' Approach To The Threat Of Chemical And Biological Warfare, Matthew Linkie

Journal of Contemporary Health Law & Policy (1985-2015)

No abstract provided.

Halogen- 2000, Andrew Mendelsohn

Halogen

Contributors include: Marie Henning, Kim Campbell, Erika Eckart, Nathan Hoks, John Grey, John Dickenson, Michael L. Johnson, Corrine Dewinter, Daniel Green, Donna Pucciani, Johanny Vasquez, Jane Mclellan, Frank Varela, B.Z. Niditch, Robert Roden, Martha Moderna Vertreach, John Payne Kincaid, C. Mulrooney, Phillip E. Saineghi, David Chorlton, Patty Dickenson Pieczyka, Mike Catalano, Tom West, Susannah Malonado, E.W. Beals, Constance Vogel, R.A. Stewart, Justin Carroll

Curio 2000 Spring, James Madison University. College Of Arts And Letters

Curio Magazine

Curio, a magazine highlighting Harrisonburg, Virginia, and its surrounding communities, is published by students in the Media Arts & Design program at James Madison University. Curio is a nonprofit organization supported by the College of Arts and Letters and the School of Media Arts & Design. Subscriptions are not available.

Biotechnology And The Creation Of Ethics, Raymond R. Coletta

McGeorge Law Review

No abstract provided.