Digital Commons Network^™

Nyhuis V. Reno

2000 Decisions

USDC for the Western District of Pennsylvania

Human Serum Albumin Enhances Dna Transfection By Lipoplexes And Confers Resistance To Inhibition By Serum, Sérgio Simões, Vladimir Slepushkin, Pedro Pires, Rogério Gaspar, Maria C. Pedroso De Lima, Nejat Düzgüneş

All Dugoni School of Dentistry Faculty Articles

Cationic liposome-DNA complexes ('lipoplexes') are used as gene delivery vehicles and may overcome some of the limitations of viral vectors for gene therapy applications. The interaction of highly positively charged lipoplexes with biological macromolecules in blood and tissues is one of the drawbacks of this system. We examined whether coating cationic liposomes with human serum albumin (HSA) could generate complexes that maintained transfection activity. The association of HSA with liposomes composed of 1,2-dioleoyl-3-(trimethylammonium) propane and dioleoylphosphatidylethanolamine, and subsequent complexation with the plasmid pCMVluc greatly increased luciferase expression in epithelial and lymphocytic cell lines above that obtained with plain lipoplexes. The …

Unhs Drew Conroy Writes Definitive Book On Oxen, Sharon Keeler

Media Relations

No abstract provided.

Efficient Gene Transfer By Transferrin Lipoplexes In The Presence Of Serum, Conchita Tros De Ilarduya, Nejat Düzgüneş

All Dugoni School of Dentistry Faculty Articles

Cationic lipids are being used increasingly as reagents for gene delivery both in vitro and in vivo. One of the limitations to the application of cationic lipid-DNA complexes (lipoplexes) in vivo is the inhibition of gene delivery by serum. In this study, we have shown that transferrin (Tf)-lipoplexes, which had transferrin adsorbed at their surface via electrostatic interactions, are much more effective than plain lipoplexes in transfecting cells in the presence of relatively high concentrations (up to 60%) of fetal bovine serum (FBS). Serum even enhanced transfection by Tf-lipoplexes composed of 1,2-dioleoyl-3-(trimethylammonium) propane (DOTAP)/dioleoylphosphatidylethanolamine (DOPE)/pCMVLacZ at high lipid/DNA (+/-) charge …

The Parthenon, February 15, 2000, Marshall University

The Parthenon

No abstract provided.

The Structure Of South Dakota Agriculture Changes And Projections, Matthew Diersen, Larry Jassen, Paula Loewe

Economics Research Reports (1969 - 2017)

Agriculture in South Dakota has changed greatly in the second half of the 20th century and many more changes will occur in the next 20 to 50 years of the 21st century. ''The technology, organization, and structure of agriculture are dynamic, and future changes may dwarf past ones. The evolution of agriculture will have important impacts on farmers and society at large." (Hallam, 1993, pg. 1 ). The purposes of this report are: (1) to examine and explain key changes in the organization and structure of South Dakota's farm sector, (2) to provide a contemporary profile of farm business and …

The Daily Egyptian, February 15, 2000, Daily Egyptian Staff

February 2000

No abstract provided.

The Pan American (2000-02-15), Ryan R. Henry

UTPA Archives - The Pan American Newspaper

48th Year, No. 29

Unhs Drew Conroy Writes Definitive Book On Oxen, Sharon Keeler

Media Relations

No abstract provided.

The Grizzly, February 15, 2000, Stephanie Restine, Jamie Matty, Cynthia Neff, Dan Reimold, Bradford Joyce, Ed Kloss, Kevin Wilson, Tim Burton, Jeremy Fox, Jeff Church, Kate Buchert, Emily Callaghan, Lauren Flanagan, Tim Noone, Lauren Cyrsky, Fran Shaughnessy, Luther Owens, Heather W. Gurk, Andy Owens

Ursinus College Grizzly Newspaper, 1978 to Present

UC Students Debate Pros, Cons of Pledging On Campus • Feelings of Brotherhood, Sisterhood Prevalent During Pledging Process • Employment Available for Graduating Seniors • True Love: Sorrow and Devotion • Hackers, Hijackers, and the Wide World of Sports • The Greeks Agree: Pledges Have no Free Will • Pledging: What's the Big Deal Anyway? • muMs Schemes at Ursinus • Pat McGee to Jam at Ursinus • Music Review: The Alligator Blues Band • Gymnastics Tops RIC with Season High Score • Intramural 3 on 3 Action: Brains vs. Brute • Indoor Track Steps Up to "Eight Way Challenge" …

Spartan Daily, February 14, 2000, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily (School of Journalism and Mass Communications)

Volume 114, Issue 12

The Statesman, V. 44, I. 08, State University Of New York At Stony Brook

Stony Brook Statesman-Sucolian

v. 44, i. 08

Fortnightly - February 14, 2000

Fortnightly

No abstract provided.

The Daily Egyptian, February 14, 2000, Daily Egyptian Staff

February 2000

No abstract provided.

Commonwealth Times 2000-02-14

Commonwealth Times, 1969-

No abstract provided.

February 14, 2000 Reporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

The Bg News February 14, 2000, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. February 14, 2000. Volume 85-Issue 24.

The George-Anne, Georgia Southern University

The George-Anne

• Ribbon Cutting Officially Opens new Art Studios
• Opinions
• Sports
• Lifestyles
• Classifieds

February 12, 2000 (Pages 755-856), Pennsylvania Legislative Reference Bureau

Volume 30 (2000)

No abstract provided.

Micro-Ct Image-Derived Metrics Quantify Arterial Wall Distensibility Reduction In A Rat Model Of Pulmonary Hypertension, Roger H. Johnson, Kelly L. Karau, Robert C. Molthen, Steven Thomas Haworth, Christopher A. Dawson

Biomedical Engineering Faculty Research and Publications

We developed methods to quantify arterial structural and mechanical properties in excised rat lungs and applied them to investigate the distensibility decrease accompanying chronic hypoxia-induced pulmonary hypertension. Lungs of control and hypertensive (three weeks 11% O₂) animals were excised and a contrast agent introduced before micro-CT imaging with a special purpose scanner. For each lung, four 3D image data sets were obtained, each at a different intra-arterial contrast agent pressure. Vessel segment diameters and lengths were measured at all levels in the arterial tree hierarchy, and these data used to generate features sensitive to distensibility changes. Results indicate …

Mustang Daily, February 11, 2000

Cal Poly Student Newspaper

Student newspaper of California Polytechnic State University, San Luis Obispo, CA.

The Daily Egyptian, February 11, 2000, Daily Egyptian Staff

February 2000

No abstract provided.

Interview With Mike Dailey, Todd Juengling, Mike Dailey

Interviews

Mikw Dailey talks about his work at the Environmental Center

Volume 37, Number 23: February 11, 2000, University Of North Dakota

University Letter Archive

No abstract provided.

February 11th 2000, Csusb

Coyote Chronicle (1984-)

No abstract provided.

The Courier, Volume 33, Issue 14, February 11, 2000, The Courier, College Of Dupage

The Courier

Stories:

First President Dies At 87

No Action Taken On Wrestling

Trustee Candidates Want More Than PE, SGA Vote

Different Impressions Of China

Students Learn Lessons In Safe Sex

Klaas Achieves 500th Career Victory

People:

Rodney Berg

Ray Berndtson

Freedy Nang

Mike O'Neil

James McGrew

Mike Folker

Susan Faludi

Joanne Brouwer

Don Klaas

Liz Wenger

Alex Sutter

The Utah Statesman, February 11, 2000, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Reporter - February 11th 2000, Rit Reporter Staff

University Publications

No abstract provided.

The Tiger Vol. 93 Issue 14 2000-02-11, Clemson University

Tiger Newspapers

No abstract provided.

The Parthenon, February 11, 2000, Marshall University

The Parthenon

No abstract provided.