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1996

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The Cytoplasmic N,N'-Diacetylchitobiase Gene From Vibrio Parahaemolyticus: Sequence Analysis, Protein Secretion, And Secretion System Development., Michael Hongli Wu Jan 1996

The Cytoplasmic N,N'-Diacetylchitobiase Gene From Vibrio Parahaemolyticus: Sequence Analysis, Protein Secretion, And Secretion System Development., Michael Hongli Wu

LSU Historical Dissertations and Theses

The nucleotide sequence of the gene encoding the cytoplasmic $N,N\sp\prime$-diacetylchitobiase from Vibrio parahaemolyticus has been determined. The deduced peptide sequence surprisingly has minimum evolutionary relationship to two other reported $N,N\sp\prime$-diacetylchitobiases from vibrios, except for highly conserved regions which are also homologous with lysosomal beta-hexosaminidases from eukaryotes including humans. In contrast, the other two sequenced chitobiases from vibrios are much more closely related to each other. This 85 kDa cytoplasmic protein, as revealed from the sequence, appears to be a unique protein, lacking a signal sequence and genetically distant from other known enzymes of similar function. This is consistent with its …

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Scale, Resolution And Resampling: Representation And Analysis Of Remotely Sensed Landscapes Across Scale In Geographic Information Systems., Stephanie Jean Weigel Jan 1996

Scale, Resolution And Resampling: Representation And Analysis Of Remotely Sensed Landscapes Across Scale In Geographic Information Systems., Stephanie Jean Weigel

LSU Historical Dissertations and Theses

Earth system scientists are increasingly using the technologies of Geographic Information Systems (GIS) and Remote Sensing (RS) in their analyses of earth system processes and patterns. These investigations take place over a wide range of scales, from the local to the global. Global change researchers focus on both the physical and human dimensions of changes in the earth's landscapes, which occur across a range of scales and may be scale dependent. The way in which landscapes are represented in GIS and RS, using specific spatial data models and data spatial resolutions, affects the subsequent analyses that can be performed. Optimally …

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Interrelationships Among Macrophomina Phaseolina, Criconemella Xenoplax, And Tylenchorhynchus Annulatus On Grain Sorghum (Sorghum Bicolor)., Ida Wenefrida Jan 1996

Interrelationships Among Macrophomina Phaseolina, Criconemella Xenoplax, And Tylenchorhynchus Annulatus On Grain Sorghum (Sorghum Bicolor)., Ida Wenefrida

LSU Historical Dissertations and Theses

Microplot experiments were established in 1992, 1993, and 1994 to investigate the relationships between the fungal pathogen Macrophomina phaseolina, and a nematode community comprised of Criconemella xenoplax and Tylenchorhynchus annulatus on grain sorghum in Louisiana. A factorial treatment arrangement was employed and consisted of two sorghum hybrids (De Kalb DK 50 and Pioneer hybrid 8333), three levels of M. phaseolina (0, 10, and 100 colony forming units (cfu)/g soil) and three nematode inoculum levels (0, X, and 2X). Nematode inoculum at X levels were 929, 1139, and 1445 for C. xenoplax and T. annulatus per pot in 1992, 1993, and …

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Interaction Between Ap-1 Proteins And Glucocorticoid Receptors In Regulation Of Lymphokine Activated Killer (Lak) Cell Activity In The Equine Species., Jing Bu Jan 1996

Interaction Between Ap-1 Proteins And Glucocorticoid Receptors In Regulation Of Lymphokine Activated Killer (Lak) Cell Activity In The Equine Species., Jing Bu

LSU Historical Dissertations and Theses

This project was performed in order to determine whether exercise-induced increases in plasma cortisol concentrations may be responsible for enhanced lymphokine activated killer (LAK) cell activity. Secondly, this project included preliminary experments which were designed to test the hypothesis that stimulatory effects of cortisol on LAK cell activity may be due to cross-talk between signalling systems important to glucocorticoid effects and stimuli which act through nuclear activator protein-1 (AP-1). Blood samples were collected from resting horses in order to determine whether the animals had normal plasma glucocorticoid concentrations. All horses included in these experiments had $10\sp{-7}$ to $10\sp{-8}$M cortisol concentrations …

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The Biology And Ecology Of The Yellowmargined Leaf Beetle, Microtheca Ochroloma Stal, (Coleoptera: Chrysomelidae) On Crucifers., Abdullahi Olod Ameen Jan 1996

The Biology And Ecology Of The Yellowmargined Leaf Beetle, Microtheca Ochroloma Stal, (Coleoptera: Chrysomelidae) On Crucifers., Abdullahi Olod Ameen

LSU Historical Dissertations and Theses

The biology and ecology of the yellowmargined leaf beetle, Microtheca ochroloma Stal, were studied on cabbage, Brassica oleracea var capitata L., collard, B. oleracea var acephala L., mustard, B. juncea Cosson, turnip, B. rapa L., and radish, Raphanus sativus L. The life cycle of the beetle consists of an egg stage, four larval instars, prepupal, pupal and adult stages. There were no significant differences in the effect of host plant on duration of development of immature beetles (p = 0.3353). The mean duration of development from oviposition to adult emergence ranged from 26.6 d on turnip to 27.5 d on …

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Immunodiagnosis Of Fasciolosis By Detection Of Coproantigen., Salma Mohamed Abdel-Rahman Jan 1996

Immunodiagnosis Of Fasciolosis By Detection Of Coproantigen., Salma Mohamed Abdel-Rahman

LSU Historical Dissertations and Theses

A monoclonal antibody (MAb)-based western blot analysis (WB) was developed for detection of a 26-28 kD coproantigen in Fasciola hepatica infected cattle. Using WB the coproantigen was detected in feces from experimentally infected calves with 22 flukes or more. Using the biotin-streptavidin modification of WB, antigen was detected in the feces from experimentally-infected calves with 10 flukes or more as early as 6 weeks post-infection. The MAbs did not react with the ES of Paramphistomum sp. or Moniezia sp, but showed slight cross-reactivity with ES from F. gigantica, and there was moderate cross-reactivity with Fascioloides magna ES. Differential staining of …

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Impact Of Insecticides On Predators Of The Soybean Looper, Pseudoplusia Includens (Walker)., Michael Lynn Boyd Jan 1996

Impact Of Insecticides On Predators Of The Soybean Looper, Pseudoplusia Includens (Walker)., Michael Lynn Boyd

LSU Historical Dissertations and Theses

Insecticide toxicity to hemipteran predators (i.e. Geocoris punctipes (Say), Nabis capsiformis Germar, Nabis roseipennis Reuter, and Podisus maculiventris (Say)) was evaluated using three different routes of insecticide exposure (direct contact, indirect insecticide toxicity through consumption of treated prey, and residue uptake). Pirate 3SC$\sp\circler$ (chlorfenapyr) had direct contact toxicity equal to Ambush 2E$\sp\circler$ (permethrin) and Methyl Parathion 4E$\sp\circler$ to most hemipteran predators tested. Exposure to foliage treated with Proclaim 0.16E$\sp\circler$ (emamectin benzoate) usually resulted in lower mortality as compared to Pirate. Condor OF 100F$\sp\circler$ (Bacillus thuringiensis) had the lowest contact toxicity to hemipteran predators of all insecticides tested. Pirate at 0.224 …

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Cloning, Sequencing And Regulation Of The Rhodobacter Capsulatusheme Gene., Georgia Ineichen Jan 1996

Cloning, Sequencing And Regulation Of The Rhodobacter Capsulatusheme Gene., Georgia Ineichen

LSU Historical Dissertations and Theses

In Rhodobacter capsulatus, a purple nonsulfur bacterium, the tetrapyrrole pathway yields four end products: siroheme, vitamin B12, heme and bacteriochlorophyll. Growth of R. capsulatus under low oxygen tension increases carbon flow through the tetrapyrrole pathway up to 100 fold. The mechanism(s) for this regulation is currently unknown. One regulatory mechanism could be transcriptional control of one or more of the genes in the common portion of the tetrapyrrole pathway. The fifth gene in the pathway, hemE, has been cloned by complementation of an Escherichia coli hemE::miniTn10 mutant. Sequence data of hemE revealed the presence of an upstream palindrome that is …

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Performance, Cultural Identity, And Feminist Practice In The Oral History Of An African-American Domestic Worker., Elondust Patrick Johnson Jan 1996

Performance, Cultural Identity, And Feminist Practice In The Oral History Of An African-American Domestic Worker., Elondust Patrick Johnson

LSU Historical Dissertations and Theses

This study analyzes the personal narrative of Mary Rhyne, an elderly African American domestic worker. The study describes the ethnographic process of collecting, transcribing and interpreting the informant's oral history. Interpretive and theoretical perspectives concerned with African American culture, domestic labor, and feminist practice are used to analyze the narrative. Further, the ethnographic process, the informant's oral "telling," and the events that she recalls and recounts are viewed as, and in terms of, performance. Performance foregrounds the collaborative and "fictional" aspects of the ethnographer-informant relationship. It directs attention toward the "performance event" as a site and situation where the teller …

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Amorphization And Fracture In Silicon Selenium(2) Nanowires: Molecular-Dynamics Simulations On Parallel Computer Architectures., Wei Li Jan 1996

Amorphization And Fracture In Silicon Selenium(2) Nanowires: Molecular-Dynamics Simulations On Parallel Computer Architectures., Wei Li

LSU Historical Dissertations and Theses

The primary goal of this dissertation is to investigate the structural and mechanical properties and dynamical fracture in SiSe$\sb2$ nanowires using the molecular-dynamics (MD) simulation technique. The present work is the first study of SiSe$\sb2$ nanowires. Large-scale simulations reported in this thesis are carried out with parallel multiresolution schemes for the long-range Coulomb and the three-body covalent potentials. The multiresolution scheme reduces the computational complexity from O(N$\sp2$) to O(N). Using an effective interatomic potential containing both 2- and 3-body interactions, MD simulations are performed for SiSe$\sb2$ nanowires composed of finite (1-64) number of chains. Under small uniaxial strain, the nanowires …

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Nesting Ecology Of The White-Faced Ibis (Plegadis Chihi) In Southwestern Louisiana., Kathleen Carson Garrett Jan 1996

Nesting Ecology Of The White-Faced Ibis (Plegadis Chihi) In Southwestern Louisiana., Kathleen Carson Garrett

LSU Historical Dissertations and Theses

I studied the nesting ecology of the White-faced Ibis (Plegadis chihi) during the summers of 1994 and 1995 in three colonies in Lacassine National Wildlife Refuge, southwestern Louisiana. Data were collected on colony-site characteristics, chronology (with abundance) of colony formation, reproductive success, and growth and development of ibis nestlings. Small colonies nested in dense buttonbush (Cephalanthus occidentalis) and water willow (Decodon verticillatus) "islands." The largest colony nested in black willows (Salix nigra) (3.2 ha)--a habitat unique to ibis populations in Louisiana--with nests heights up to 7.5 m. Cattle egrets (Bubulcus ibis) (62%) and ibises (36%) were the most abundant species. …

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Importance Of A Siderophore In The Pathogenesis And Virulence Of Photobacterium Damsela Subsp. Piscicida In Hybrid Striped Bass (Morone Saxatili X Morone Chrysops)., John Phillip Hawke Jan 1996

Importance Of A Siderophore In The Pathogenesis And Virulence Of Photobacterium Damsela Subsp. Piscicida In Hybrid Striped Bass (Morone Saxatili X Morone Chrysops)., John Phillip Hawke

LSU Historical Dissertations and Theses

Photobacterium damsela subsp. piscicida, formerly Pasteurella piscicida, is an important new pathogen of hybrid striped bass cultured in brackish water on Louisiana mariculture farms. Louisiana isolates compared with strains of P. damsela subsp. piscicida from Greece, Japan, Israel and Chesapeake Bay, USA and were found to be identical in biochemical phenotype and enzymic activity. Small plasmids of 8 and 5 kb were unique to Louisiana strains. Sequential daily bacteriology and histopathology on hybrid striped bass experimentally infected by immersion revealed that following high doses (13,000 CFU/ml) the gills and spleen are colonized first as early as 24 hours PI, followed …

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Xanthomonas Albilineans Detection, Identification And Infectivity Titration In Sugarcane., Silvio Aparecido Lopes Jan 1996

Xanthomonas Albilineans Detection, Identification And Infectivity Titration In Sugarcane., Silvio Aparecido Lopes

LSU Historical Dissertations and Theses

A PCR method with primers for t-RNA gene spacers was used for detection of Xanthomonas albilineans (Xa), the causal agent of leaf scald disease of sugarcane. A DNA segment of approximately 170 bp was amplified for Xa strains, but also for X. campestris pv. campestris, X. campestris pv. translucens and two sugarcane bacterial endophytes. Good agreement between PCR and isolation to detect Xa from sugarcane sap was observed. PCR was faster allowing pathogen detection in less than one day. Both methods resulted in low detection frequencies from asymptomatic plants, which may contain Xa below detection limits. The lowest limit for …

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Reflection On Teaching: An Ethnographic Study Of Preservice Teachers' Beliefs And Practices., Thomasine Haskins Mencer Jan 1996

Reflection On Teaching: An Ethnographic Study Of Preservice Teachers' Beliefs And Practices., Thomasine Haskins Mencer

LSU Historical Dissertations and Theses

The purpose of this study was to explore the relationship between preservice teachers' expressed beliefs, their practices, and how they described the two. Particular emphasis was given to factors that influenced the preservice teachers' expectations of students. The preservice teachers were in the graduate year of a five year teacher education program that embraced reflective practice. In many ways, the two preservice teachers conformed to the mold of the typical American teacher in that they were middle class, Anglo Saxon, females who were high achievers from small towns. However, the pair differed in their experiences with diversity. The preservice teachers' …

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Experimental Lymphatic Filariasis In Gerbils (Meriones Unguiculatus): Molecular Cloning And Expression Of Gerbil Cytokines And Measurement Of Cytokine Gene Expression During A Primary Infection Of Brugia Pahangi., Zhiming Mai Jan 1996

Experimental Lymphatic Filariasis In Gerbils (Meriones Unguiculatus): Molecular Cloning And Expression Of Gerbil Cytokines And Measurement Of Cytokine Gene Expression During A Primary Infection Of Brugia Pahangi., Zhiming Mai

LSU Historical Dissertations and Theses

Cytokines play an important role in regulation of T cell responses. Two T helper cell subsets have been identified in some species and are defined by their cytokine secretion profiles. Th1 cells produce IL-2 and IFN-$\gamma,$ whereas Th2 cells express IL-4, IL-5, and IL-10. Precursor T cells that express a mixture of these cytokines are considered Th0 cells. It is hypothesized that primary infections of Brugia pahangi in gerbils induce an initial Th0 or Th1 like response which with time becomes a Th2 like response. To test this dynamic parasitism induced shift in cytokine profile, it is necessary to measure …

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Effect Of Diet On Respiratory Fuel Mixture, Insulin Sensitivity, And Autonomic Nervous System Function., Heli J. Roy Jan 1996

Effect Of Diet On Respiratory Fuel Mixture, Insulin Sensitivity, And Autonomic Nervous System Function., Heli J. Roy

LSU Historical Dissertations and Theses

Introduction. A high amount of dietary fat has been shown to have a negative effect on metabolism, causing insulin resistance, weight gain, and other metabolic alterations. Several studies have found a direct link between lack of activity and obesity. Purpose. The purpose of this study was to see if a diet had an effects on insulin sensitivity, autonomic nervous system function, and substrate utilization as predicted by Flatt, in healthy young men. Subjects. The subjects studied were 6 nonathletes (NA), 6 aerobically trained (AT), and 5 weight trained (WT) men. Their body mass index (BMI) was 22.1 $\pm$ 2.92 kg/m$\sp2$ …

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Variability Of Eddy Heat Fluxes Over The Northwestern Gulf Of Mexico., Varis Ransibrahmanakul Jan 1996

Variability Of Eddy Heat Fluxes Over The Northwestern Gulf Of Mexico., Varis Ransibrahmanakul

LSU Historical Dissertations and Theses

Eddy heat flux variability over the Louisiana-Texas shelf was investigated using satellite-derived surface velocity and temperature data between October 1993 and October 1994. Assuming the product of sea water density and specific heat is relatively constant, velocity-temperature covariance reflects eddy heat flux (the fluctuating part of the 1 x 1 degree, 90 day mean heat flux). Available velocity and temperature fields, however, are not synchronous. Temperature "observations" at velocity positions were optimally estimated using the Gauss Markoff Theorem. The error-estimate is comparable to the error resulting from the application of the widely accepted SST correction algorithm. The trend that instantaneous …

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Breeding Ecology Of Songbirds In Bottomland Hardwood Forests Of Louisiana., Keith Ouchley Jan 1996

Breeding Ecology Of Songbirds In Bottomland Hardwood Forests Of Louisiana., Keith Ouchley

LSU Historical Dissertations and Theses

I examined productivity, survivorship and nest-site habitat characteristics of small terrestrial landbirds breeding in bottomland hardwood forest study sites in Louisiana. Bottomland hardwood forests are the dominate ecosystem of riverine floodplains in the southeastern United States, and these forests support a diverse bird community. Over 80 percent of this forest type has been lost, primarily due to agricultural clearing, and the relative abundance of many bird species that breed in these forests has also declined. The outcomes of 790 nests of 33 species were determined. For most species, productivity was greater in large forest tracts than in small tracts. Predation …

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Inflammatory Responses Of The Jird To Brugia Pahangi: Parasite Stage Specificity And Role Of The Macrophage., Carmen Nasarre Jan 1996

Inflammatory Responses Of The Jird To Brugia Pahangi: Parasite Stage Specificity And Role Of The Macrophage., Carmen Nasarre

LSU Historical Dissertations and Theses

The host systemic and peritoneal inflammatory responses against different stages of Brugia pahangi were compared in intraperitoneally infected-jirds. Systemic inflammatory responses were assessed by measuring the pulmonary granulomatous response to B. pahangi antigen-coated beads (PGRN). Peritoneal inflammation was characterized by enumeration of different cell types in peritoneal exudates following infection. Further, the toxoplasmacidal activity and TNF-$\alpha$-like production of peritoneal macrophages were also characterized. Infection with L3, L4, male worms, female worms, microfilariae (MF) or dead adult worms induced a rapid PGRN. This response decreased to levels of controls in jirds inoculated with living parasites at the chronic time period, indicating …

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Biological Control Of Pythiaceous Fungi Causing Death Of Longleaf Pine Seedlings During Cold Storage., Xiaoan Sun Jan 1996

Biological Control Of Pythiaceous Fungi Causing Death Of Longleaf Pine Seedlings During Cold Storage., Xiaoan Sun

LSU Historical Dissertations and Theses

Pythium dimorphum Hendrix & Campbell and other Pythium species were determined to be pathogenic fungi that cause the death of longleaf pine seedlings during cold storage. Pathogenicity of these Pythium isolates as determined by infecting newly-germinated slash pine seedlings in vitro was confirmed in field evaluations. An in vitro dual culture screening system was effectively used for selecting Trichoderma isolates capable of killing P. dimorphum. Selected Trichoderma isolates and Gliocladium virens produced volatile and/or non-volatile antibiotics on solid media and in liquid cultures, but antibiotic production could be affected by medium type, pH, and culture age, depending on species or …

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Modeling Physical And Chemical Nonequilibrium Transport Of Herbicide In Soils From Different Tillage Systems., Shikui Xue Jan 1996

Modeling Physical And Chemical Nonequilibrium Transport Of Herbicide In Soils From Different Tillage Systems., Shikui Xue

LSU Historical Dissertations and Theses

The physical and chemical nonequilibrium transport of alachlor were studied in a surface Gigger soil from different tillages through tracer studies, and batch and miscible displacement experiments. Batch experiments indicated initially fast reaction followed by slow adsorption. Adsorption and desorption results indicated time dependent hysteretic behavior and was best described by a multireaction model incorporating nonlinear equilibrium reaction, a reversible kinetic mechanism, and a consecutive irreversible mechanism. The model predicted alachlor hysteresis and adsorption-desorption kinetics satisfactorily based on parameters obtained from adsorption experiments. Tracer (Eosin Y and Blue FCF dyes) studies showed non-uniformly stained areas in undisturbed soil cores (6.4 …

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Pilot-Scale Solvent Sublation For The Removal Of Hydrophobic Compounds From Wastewaters., Jeffrey Scott Smith Jan 1996

Pilot-Scale Solvent Sublation For The Removal Of Hydrophobic Compounds From Wastewaters., Jeffrey Scott Smith

LSU Historical Dissertations and Theses

Solvent sublation is a nonfoaming adsorptive bubble process which exploits the surface active nature of organic compounds in their removal from aqueous systems. Gas bubbles are introduced into an aqueous media and are buoyed upward to an immiscible organic solvent. As the bubbles rise, organic materials are selectively adsorbed from the aqueous phase and transported to the overlying, quiescent solvent layer. This dissertation studied three-phase, continuous solvent sublation of pyrene and pentachlorophenol from simulated wastewater at the pilot-scale. Steady-state removal efficiencies as high as 96% for pyrene and 94% for pentachlorophenol were demonstrated and were significantly greater than the corresponding …

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The Role Of Seed Banks, Disturbance, And Sea Level Rise In Determining The Plant Community Structure Of Oligohaline Coastal Marshes., Andrew Hamilton Baldwin Jan 1996

The Role Of Seed Banks, Disturbance, And Sea Level Rise In Determining The Plant Community Structure Of Oligohaline Coastal Marshes., Andrew Hamilton Baldwin

LSU Historical Dissertations and Theses

Coastal wetlands worldwide are threatened by sea level rise, which is expected to affect the growth and survival of wetland vegetation by increasing water level and salinity. While the effects of salinity and inundation on adult vegetation of oligohaline marshes have been widely studied, the species composition of the seed bank and its response to elevated salinity and water level have not been examined. Additionally, the role of disturbances of different intensities in structuring marsh plant communities and possible interactions between disturbance and rising sea level have received little attention. Disturbances in coastal marshes include nonlethal disturbances such as fire …

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The Impact Of Total Sleep Deprivation On Neuropsychological Functioning., Paul Gale Binks Jan 1996

The Impact Of Total Sleep Deprivation On Neuropsychological Functioning., Paul Gale Binks

LSU Historical Dissertations and Theses

Extended sleep deprivation has been shown to produce impairments in sustained attention and vigilance, especially if the deprivation period is greater than 48 hours. However little is known about the effects of sleep deprivation on performance of cognitive tasks considered to be measures of higher cortical functioning such as cognitive flexibility and the capacity to shift response set. These two activities are associated with intact functioning of the frontal lobes of the cerebral cortex while attention and vigilance tasks are not considered to be part of this type of cognitive activity and are not associated with frontal lobe function. One …

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The Relationship Of Hemolymph Sugar, Juvenile Hormone And Biogenic Amines To The Three Behavioral States Of Mature Honey Bees., Janko Bozic Jan 1996

The Relationship Of Hemolymph Sugar, Juvenile Hormone And Biogenic Amines To The Three Behavioral States Of Mature Honey Bees., Janko Bozic

LSU Historical Dissertations and Theses

Differences in sugar titers, JH synthesis rate and biogenic amine metabolism correlated with three distinct activity groups or mature bees: resting bees, followers and dancers. Dancers had lower trehalose titers than followers or resting bees. In absconding bees trehalose titers were even lower, due to greatly elevated locomotor activity. The collecting and transferring of food by dancers and followers resulted in a higher glucose titer than in resting bees. Followers had a higher-JH synthesis rate than dancers, suggesting that JH could be an internal motivational stimulus to recruit followers to forage. Dancers and followers collect and transfer food and therefore …

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Work Motivation And Perceived Organizational Effectiveness In Middle Schools., Cynthia Young Wren Jan 1996

Work Motivation And Perceived Organizational Effectiveness In Middle Schools., Cynthia Young Wren

LSU Historical Dissertations and Theses

The primary purpose of this study was to determine the linkage between work motivation derived from the expectancy theory and perceived organizational effectiveness derived from the Parsonian framework. School characteristics of community type, school size, and school socioeconomic status were used as predictor variables in the study. A secondary purpose was to examine these linkages in middle schools and to examine changes over time. Mixed methodologies, including quantitative and qualitative research techniques, were employed. The data were analyzed using the school and the individual teacher as units of analyses. To test the hypotheses, a multiple regression procedure was employed. The …

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Robust Sensor Fusion Algorithms: Calibration And Cost Minimization., Richard Ree Brooks Jan 1996

Robust Sensor Fusion Algorithms: Calibration And Cost Minimization., Richard Ree Brooks

LSU Historical Dissertations and Theses

A system reacting to its environment requires sensor input to model the environment. Unfortunately, sensors are electromechanical devices subject to physical limitations. It is challenging for a system to robustly evaluate sensor data which is of questionable accuracy and dependability. Sensor fusion addresses this problem by taking inputs from several sensors and merging the individual sensor readings into a single logical reading. The use of heterogeneous physical sensors allows a logical sensor to be less sensitive to the limitations of any single sensor technology, and the use of multiple identical sensors allows the system to tolerate failures of some of …

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An Attempt To Classify The Production And Regulation Of Cyclooxygenase-2 During Ovulation And Luteolysis., James Robert Broussard Jan 1996

An Attempt To Classify The Production And Regulation Of Cyclooxygenase-2 During Ovulation And Luteolysis., James Robert Broussard

LSU Historical Dissertations and Theses

A series of experiments were designed to determine the cyclooxygenase (COX) isoform responsible for prostaglandin release during ovulation and luteolysis. Initially, a reliable, serum-free culture system was developed to produce large numbers of bovine granulosa cells originally harvested from the small antral follicles of abattoir ovaries. This method allowed adequate numbers of cells to be obtained for the subsequent study involving cyclooxygenase regulation. In a second experiment, bovine granulosa cells from small antral follicles were isolated and cultured as in the first experiment for 6 days. Granulosa cells were then exposed to medium alone or medium containing arachidonic acid (10 …

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Nitrogen Accumulation By Rice., Arthur Bufogle Jr Jan 1996

Nitrogen Accumulation By Rice., Arthur Bufogle Jr

LSU Historical Dissertations and Theses

Consumption of rice is worldwide, and the rice-eating population is expanding rapidly. Breeding high yielding varieties has helped increase rice production. However, effective management practices have also been useful, and will probably become more vital as breeding efforts approach the genetic limits of the rice plant. Nitrogen (N) management is particularly important in reducing N losses and increasing grain yield. The objectives of this study were (1) to determine the amount of N accumulation by rice at different stages of plant development, (2) to determine if differences in N accumulation exist between rice varieties, and (3) to determine if differences …

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The Effect Of Membrane Structure On Lipid Peroxidation And Its Inhibition., Tammy Renee Dugas Jan 1996

The Effect Of Membrane Structure On Lipid Peroxidation And Its Inhibition., Tammy Renee Dugas

LSU Historical Dissertations and Theses

Lipid peroxidation is the process by which lipids in the cell membrane undergo free radical-initiated oxidation. The resulting cell damage has been implicated in the onset of many diseases, including as cancer, aging, and stroke. Lipid oxidation has been studied in a variety of membrane models, including homogeneous solution, micelles, liposomes, and microsomes. The kinetics for free radical reactions proven to occur during lipid oxidation in homogeneous solution have been thoroughly elucidated. These kinetics were also shown to apply to oxidation in micelles. This dissertation presents evidence that the kinetics of more biologically realistic membrane models such as liposomes may …

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