Digital Commons Network^™

Rabies, Canine Distemper, And Canine Parvovirus Exposure In Large Carnivore Communities From Two Zambian Ecosystems, Are R. Berentsen, Mike R. Dunbar, Matthew S. Becker, Jassiel M'Soka, Egil Droge, Nicholas M. Sakuya, Wigganson Matandiko, Rachel Mcrobb, Cathleen A. Hanlon

United States Department of Agriculture Wildlife Services: Staff Publications

Disease transmission within and among wild and domestic carnivores can have significant impacts on populations, particularly for threatened and endangered species. We used serology to evaluate potential exposure to rabies virus, canine distemper virus (CDV), and canine parvovirus (CPV) for populations of African lions (Panthera leo), African wild dogs (Lycaon pictus), and spotted hyenas (Crocuta crocuta) in Zambia’s South Luangwa National Park (SLNP) and Liuwa Plain National Park (LPNP) as well as community lands bordering these areas. In addition, domestic dogs in the study region were evaluated for exposure to CDV and rabies. We provide …

Population Management To Reduce The Risk Of Wiid Life-Aircraft Collisions, Richard A. Dolbeer, Alan B. Franklin

United States Department of Agriculture Wildlife Services: Staff Publications

Pour basic control strategies mitigate the risks to aviation caused by wildlife at airports: (1) aircraft flight schedule modification (primarily at military air· bases) and enhancement of aircraft visibility to avoid interactions with wildlife (e.g., Blackwell et a1. 2009b, 2012); (2) habitat modification and elimination of food, water, and cover that attract wildlife (Cleary and Dolbeer 2005, Blackwell et al. 2009a; Chapters 5, 8-10); (3) repellent and harassment techniques to disperse wild life (Cleary and Dolbeer 2005; Chapters 2-4); and (4) wildlife population management (e.g., Dolbeer 1998). As discussed throughout this book, successful efforts to mitigate the risk of wildlife-aircraft …

Prevalence Of Avian Paramyxovirus 1 And Avian Influenza Virus In Double-Crested Cormorants (Phalacrocorax Auritus) In Eastern North America, Tiffanie A. Cross, D. M. Arsnoe, R. B. Minnis, D. T. King, S. Swafford, K. Pedersen, J. C. Owen

United States Department of Agriculture Wildlife Services: Staff Publications

Although it is well established that wild birds, such as cormorants, carry virulent avian paramyxovirus serotype 1 (APMV-1; causative agent of Newcastle disease) and avian influenza virus (AIV), the prevalence of these viruses among Double-crested Cormorants (Phalacrocorax auritus) in the Great Lakes region of North America has not been rigorously studied. We determined the prevalences of APMV-1 and AIV in Double-crested Cormorants from the interior population of eastern North America. From 2009 to 2011, oropharyngeal and cloacal swabs and serum samples were collected from 1,957 individual Double-crested Cormorants, ranging from chicks to breeding adults, on breeding colony sites …

Understanding Animal Movements At And Near Airports, Jerrold L. Belant, Brian E. Washburn, Travis L. Devault

United States Department of Agriculture Wildlife Services: Staff Publications

Understanding movements of hazardous wildlife species at and near airports is critical to formulating effective management strategies for reducing aviation risk. Animal movements vary daily, seasonally, and annually and are based on broad biological and ecological concepts, including foraging, reproduction, habitat characteristics, dispersal, and migration. As an energy conservation strategy. most animals minimize their movements to meet life requisites, which in turn presumably improves fitness. Animal movements in relation to airports can be direct; for example, Canada geese (Branta canadensis) flying onto an airfield because grass height and composition are suitable for loafing sites and as food. Animal …

Determination Of An Immunocontraceptive Peptide In A Wildlife Vaccine Formulation, David A. Goldade, Jeffrey M. Kemp, Jeanette R. O'Hare, Lowell A. Miller

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife populations continue to grow despite the use of traditional management techniques. GonaCon™ Immunocontraceptive Vaccine is a vaccine used to reduce reproduction in mammalian species, including white-tailed deer (Odocoileus virginianus). The vaccine consists of synthetic gonadotropin releasing hormone (GnRH) conjugated to a mollusk hemocyanin (Concholepas concholepas) prepared as an emulsion with mineral oil to promote a prolonged immune response. Development of an analytical method for determination of the active ingredient in the vaccine formulation was complicated by the emulsion and conjugation of GnRH to the carrier protein. Breaking the emulsion was achieved chemically by addition of …

Procedures For Identifying Infectious Prions After Passage Through The Digestive System Of An Avian Species, Justin W. Fischer, Tracy A. Nichols, Gregory E. Phillips, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Infectious prion (PrP^Res) material is likely the cause of fatal, neurodegenerative transmissible spongiform encephalopathy (TSE) diseases¹. Transmission of TSE diseases, such as chronic wasting disease (CWD), is presumed to be from animal to animal^2,3 as well as from environmental sources^4-6. Scavengers and carnivores have potential to translocate PrP^Res material through consumption and excretion of CWD contaminated carrion. Recent work has documented passage of PrP^Res material through the digestive system of American crows (Corvus brachyrhynchos), a common North American scavenger⁷.

We describe procedures used to document passage of …

Brownian Bridge Movement Models To Characterize Birds’ Home Ranges, Justin W. Fischer, W. David Walter, Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

A Brownian bridge movement model (BBMM) is a relatively new concept that estimates the path of an animal’s movement probabilistically from data recorded at brief intervals. A BBMM assumes that locations are not independent, whereas the “classical” kernel-density estimator (KDE) assumes they are. We estimated BBMM home ranges for 11 Black Vultures (Coragyps atratus) and 7 Turkey Vultures (Cathartes aura) equipped with satellite transmitters near Marine Corps Air Station Beaufort, South Carolina, from October 2006 to November 2008. The 95% BBMM home ranges (95% BBMM) of the two Black Vultures that traveled >100 km from the …

Avian Influenza Infection Alters Fecal Odor In Mallards, Bruce A. Kimball, Kunio Yamazaki, Dennis Kohler, Richard A. Bowen, Jack P. Muth, Maryanne Opiekun, Gary K. Beauchamp

United States Department of Agriculture Wildlife Services: Staff Publications

Changes in body odor are known to be a consequence of many diseases. Much of the published work on disease-related and body odor changes has involved parasites and certain cancers. Much less studied have been viral diseases, possibly due to an absence of good animal model systems. Here we studied possible alteration of fecal odors in animals infected with avian influenza viruses (AIV). In a behavioral study, inbred C57BL/6 mice were trained in a standard Y-maze to discriminate odors emanating from feces collected from mallard ducks (Anas platyrhynchos) infected with low-pathogenic avian influenza virus compared to fecal odors …

Ecology Of Zoonotic Infectious Diseases In Bats: Current Knowledge And Future Directions, D. T. S. Hayman, R. A. Bowen, P. M. Cryan, G. M. Mccracken, T. J. O'Shea, A. J. Peel, A. T. Gilbert, C. T. Webb, J. L. N. Wood

United States Department of Agriculture Wildlife Services: Staff Publications

Bats are hosts to a range of zoonotic and potentially zoonotic pathogens. Human activities that increase exposure to bats will likely increase the opportunity for infections to spill over in the future. Ecological drivers of pathogen spillover and emergence in novel hosts, including humans, involve a complex mixture of processes, and understanding these complexities may aid in predicting spillover. In particular, only once the pathogen and host ecologies are known can the impacts of anthropogenic changes be fully appreciated. Cross-disciplinary approaches are required to understand how host and pathogen ecology interact. Bats differ from other sylvatic disease reservoirs because of …

Pheromonal Control Of The Invasive Brown Treesnake: Potency Of Female Sexual Attractiveness Pheromone Varies With Ovarian State, Tom Mathies, Breanna Levine, Richard M. Engeman, Julie A. Savidge

United States Department of Agriculture Wildlife Services: Staff Publications

The female sex pheromone has potential a s a control tool for the Brown Treesnake, Boiga irregularis Merrem, a pest introduced into Guam. We conducted male-guided bioassays to determine whether potency of the female pheromone varied according to reproductive state and during vitellogenesis. In weekly simultaneous choice tests, we presented males with skin lipid samples from vitellogenic females, non-vitellogenic females, conspecific males, and carrier controls. The total time each sample was tongue-flicked was recorded: mean total tongue-flick time was highest for samples from vitellogenic females followed by samples from non-vitellogenic females. Pair-wise comparisons of male tongue-flick time for samples of …

Feral Swine Brucellosis In The United States And Prospective Genomic Techniques For Disease Epidemiology, Owen P. Leiser, Joseph L. Corn, Brandon S. Schmit, Paul S. Keim, Jeffrey T. Foster

United States Department of Agriculture Wildlife Services: Staff Publications

Brucellosis is a common infection of feral swine throughout the United States. With the recent expansion of feral swine populations across the country, this disease poses an increasing threat to agriculture and hunters. The standard approach to Brucella surveillance in feral swine has been serological testing, which gives an indication of past exposure and is a rapid method of determining populations where Brucella is present. More in-depth analyses require bacterial isolation to determine the Brucella species and biovar involved. Ultimately, for a comprehensive understanding of Brucella epizootiology in feral swine, incorporation of genotyping assays has become essential. Fortunately, the past …

Home Ranges And Habitat Use Of Brown Pelicans (Pelecanus Occidentalis) In The Northern Gulf Of Mexico, D. Tommy King, Buddy L. Goatchter, Justin W. Fischer, John Stanton, James M. Lacour, Scott C. Lemmons, Guiming Wang

United States Department of Agriculture Wildlife Services: Staff Publications

Little is known about movements and habitat use of Brown Pelicans (Pelecanus occidentalis) in the northern Gulf of Mexico. We attached satellite transmitters to 18 adult Brown Pelicans (nine males, nine females) that were captured on Grand Isle along the Louisiana coast during 31 August-2 September 2010. Their movements and habitat use were tracked between September 2010 and March 2012. Nine of the Brown Pelicans remained proximate to the Louisiana coast; four ranged along the coasts of Alabama, Mississippi, and Louisiana; three moved from Louisiana to Texas; and two migrated across the Gulf of Mexico to the Yucatan Peninsula. …

The Use Of Contraception As A Disease Management Tool In Wildlife, Jack C. Rhyan, Lowell A. Miller, Kathleen A. Fagerstone

United States Department of Agriculture Wildlife Services: Staff Publications

Contraception offers potential as a tool for managing certain diseases in wildlife, most notably venereally transmitted diseases or diseases transmitted at parturition. Brucellosis is an excellent example of an infectious disease present in wild populations that could potentially be managed through immunocontraception. Previous studies in bison (Bison bison) suggest that a single injection of GonaCon^TM (National Wildlife Research Center, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/Wildlife Services, Fort Collins, Colorado 80521, USA) results in 3 or more yr of infertility. Ongoing studies will determine if the use of GonaCon in bison decreases shedding of Brucella …

Failure Of Transmission Of Low-Pathogenic Avian Influenza Virus Between Mallards And Freshwater Snails: An Experimental Evaluation, Paul T. Oesterle, Kathryn P. Huyvaert, Darcy Orahood, Nicole Mooers, Heather Sullivan, Alan B. Franklin, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

In aquatic bird populations, the ability of avian influenza (AI) viruses to remain infectious in water for extended periods provides a mechanism that allows viral transmission to occur long after shedding birds have left the area. However, this also exposes other aquatic organisms, including freshwater invertebrates, to AI viruses. Previous researchers found that AI viral RNA can be sequestered in snail tissues. Using an experimental approach, we determined whether freshwater snails (Physa acuta and Physa gyrina) can infect waterfowl with AI viruses by serving as a means of transmission between infected and naı¨ve waterfowl via ingestion. In our …

Twenty Years Of Immunocontraceptive Research: Lessons Learned, Lowell A. Miller, Kathleen A. Fagerstone, Douglas C. Eckery

United States Department of Agriculture Wildlife Services: Staff Publications

The National Wildlife Research Center (NWRC) began immunocontraception vaccine research by testing porcine zona pellucida (PZP) on white-tailed deer (Odocoileus virginianus). Early PZP research demonstrated that PZP induced infertility; however, increased length of the rut was observed in PZP-treated deer. An alternative vaccine using a keyhole limpet hemocyanin-gonadotropin-releasing hormone (KLH-GnRH) conjugate formulated with modified Freund’s adjuvant was developed at NWRC. Suppression of GnRH has reduced reproduction in both sexes but is most effective in females. This vaccine was effective in preventing contraception in female deer for several years after a prime and boost. Due to adverse side effects …

Effects Of Black-Tailed Deer And Roosevelt Elk Herbivory In Intensively Managed Douglas-Fir Plantations, Jimmy D. Taylor Ii

United States Department of Agriculture Wildlife Services: Staff Publications

Black-tailed deer and Roosevelt elk are found throughout conifer-dominated Pacific Northwest forests west of the Cascade Crest, where they are important aesthetically, culturally, ecologically, and recreationally. Throughout their annual cycles, deer and elk use a variety of forest types and age classes to meet their basic requirements: food, water, cover, breeding, and young-rearing. Although their foraging strategies differ, black-tailed deer (browsers) and Roosevelt elk (grazers) often use the same forests. In general, forage plants for deer and elk are shade intolerant and are stimulated to grow when exposed to direct sunlight. As such, deer and elk often use clearcut patches …

Wildlife In Managed Forests: An Overview Of Perspectives From The Pacific Northwest, Jimmy D. Taylor Ii

United States Department of Agriculture Wildlife Services: Staff Publications

1. Introduction- Humans rely on forests for fiber, food, water, shelter, recreation, inspiration, and various other benefits. Similarly, many species of native fauna depend on forests to meet their seasonal and annual requirements. Forests in the Pacific Northwest have a longstanding history of providing high quality forest products, and ownership across a complex matrix of forest stands is divided among provincial, federal, state, tribal, and private entities. Over time, requirements to provide forest products have increasingly rested with industrial and non-industrial private landowners. Concomitantly, forest management has evolved and intensified on private lands in response to increases in national and …

Landslides Cause Spatial And Temporal Gradients At Multiple Scales In The Luquillo Mountains Of Puerto Rico, Aaron B. Shiels, Lawrence R. Walker

United States Department of Agriculture Wildlife Services: Staff Publications

Landslides represent one of the most severe disturbances in montane forests because the main consequence of their occurrence is loss or downslope redistribution of the majority of the above- and below-ground biomass. We examined among-landslide gradients (size, slope, aspect, age, elevation) on 142 landslides in the Luquillo Mountains, Puerto Rico, created by three storms in 2003–2004. We also examined within-landslide gradients (top to bottom, edge to center, successional development) by reviewing 20 yr of landslide data in the Luquillo Mountains. Landslide abundance and plant successional patterns do not closely reflect the elevation gradient that is characteristic of this mountain range, …

Estimating Prion Adsorption Capacity Of Soil By Bioassay Of Subtracted Infectivity From Complex Solutions (Basics), A. Christy Wyckoff, Krista L. Lockwood, Crystal Meyerett-Reid, Brady A. Michel, Heather Bender, Kurt C. Vercauteren, Mark D. Zabel

United States Department of Agriculture Wildlife Services: Staff Publications

Prions, the infectious agent of scrapie, chronic wasting disease and other transmissible spongiform encephalopathies, are misfolded proteins that are highly stable and resistant to degradation. Prions are known to associate with clay and other soil components, enhancing their persistence and surprisingly, transmissibility. Currently, few detection and quantification methods exist for prions in soil, hindering an understanding of prion persistence and infectivity in the environment. Variability in apparent infectious titers of prions when bound to soil has complicated attempts to quantify the binding capacity of soil for prion infectivity. Here, we quantify the prion adsorption capacity of whole, sandy loam soil …

Surveillance And Movements Of Virginia Opossum (Didelphis Virginiana) In The Bovine Tuberculosis Region Of Michigan, W. D. Walter, Justin W. Fischer, C. W. Anderson, D. R. Marks, T. J. Deliberto, S. Robbe-Austerman, K. C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife reservoir hosts of bovine tuberculosis (bTB) include Eurasian badgers (Meles meles) and brushtail possum (Trichosurus vulpecula) in the UK and New Zealand, respectively. Similar species warrant further investigation in the northern lower peninsula of Michigan, USA due to the continued presence of bTB on cattle farms. Most research in Michigan, USA has focused on interactions between white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus) for the transmission of the infectious agent of bTB, Mycobacterium bovis, due to high deer densities and feeding practices. However, limited data are available on medium-sized …

West Nile Virus Infection In American Robins: New Insights On Dose Response, Kaci K. Vandalen, Jeffrey S. Hall, Larry Clark, Robert G. Mclean, Cynthia Smeraski

United States Department of Agriculture Wildlife Services: Staff Publications

West Nile virus (WNV) is a vector-borne pathogen that was first detected in the United States in 1999. The natural transmission cycle of WNV involves mosquito vectors and avian hosts, which vary in their competency to transmit the virus. American robins are an abundant backyard species in the United States and appear to have an important role in the amplification and dissemination of WNV. In this study we examine the response of American robins to infection with various WNV doses within the range of those administered by some natural mosquito vectors. Thirty American robins were assigned a WNV dosage treatment …

Open Field Test With Avipel Bird Repellent: Year I, Megan Niner, George M. Linz, Jeffrey Homan, Mark Clark

United States Department of Agriculture Wildlife Services: Staff Publications

Since the mid-1900s, blackbird depredation has become an expensive problem for farmers in the Great Plains region of the United States and Canada (Blackwell et al. 2003). Damage to crops from blackbirds costs the growers of sunflower millions annually (Peer et al. 2003). The problem stems from two concurrent events: ripening of sunflowers and fledging of millions of resident blackbirds that must quickly prepare for migration to southern wintering grounds. Multiple methods of protecting crops exist, but none have proved to be cost-effective. For this reason and the safety of the non-target species, a chemical-based repellent might be a good …

Factors Affecting Survival And Cause-Specific Mortality Of Saiga Calves In Mongolia, Bayarbaatar Buuveibaatar, Julie K. Young, Joel Berger, Amanda E. Fine, Badamjav Lkhagvasuren, Peter Zahler, Todd K. Fuller

United States Department of Agriculture Wildlife Services: Staff Publications

Factors affecting juvenile survival are poorly known in the world’s most northern antelope, the endangered saiga (Saiga tatarica), yet these factors are fundamental for understanding what drives population change. We monitored Mongolia saiga (S. tatarica mongolica) calves in Sharga Nature Reserve, western Mongolia, during 2008–2010. Our results showed that male and single calves were heavier than females and twins, respectively. However, we identified no significant differences in seasonal or annual survival rates between sexes or between singletons and twins. Litter size and birth mass varied among years, and there was a negative relationship between these variables. …

Multidisciplinary Approach To Epizootiology And Pathogenesis Of Bat Rabies Viruses In The United States, J. A. Ellison, S. R. Johnson, N. Kuzmina, A. T. Gilbert, W. C. Carson, Kurt C. Vercauteren, C. E. Rupprecht

United States Department of Agriculture Wildlife Services: Staff Publications

Zoonotic disease surveillance is typically initiated after an animal pathogen has caused disease in humans. Early detection of potentially high-risk pathogens within animal hosts may facilitate medical interventions to cope with an emerging disease. To effectively spillover to a novel host, a pathogen may undergo genetic changes resulting in varying transmission potential in the new host and potentially to humans. Rabies virus (RABV) is one model pathogen to consider for studying the dynamics of emerging infectious diseases under both laboratory and field conditions. The evolutionary history of RABV is characterized by regularly documented spillover infections and a series of notable …

Assessing Spatial Variation And Overall Density Of Aerially Broadcast Toxic Bait During A Rat Eradication On Palmyra Atoll, Richard M. Engeman, William C. Pitt, Are R. Berentsen, John D. Eisemann

United States Department of Agriculture Wildlife Services: Staff Publications

Baits containing brodifacoum rodenticide were aerially applied to eradicate invasive black rats from Palmyra Atoll, an important biodiversity center. Bait application must be sufficient to be effective, while minimizing environmental hazards by not exceeding designated label rates, prompting our bait density assessments for two aerial drops. With few physical or human resources on this remote, uninhabited atoll, assessments were particularly challenging, requiring observations within 30 min of aerial application to avoid bait loss to rats, crabs, or elements. We estimated bait density using quadrat sampling within 13 terrestrial sampling areas. We also sampled 10 tidal flat areas to assess inadvertent …

An Analytical Framework For Quantifying And Testing Patterns Of Temporal Dynamics In Social Networks, Elizabeth A. Hobson, Michael L. Avery, Timothy F. Wright

United States Department of Agriculture Wildlife Services: Staff Publications

Change is fundamental to all social systems. Temporal dynamics are critical in understanding how relationships form and change over time but rarely are studied explicitly in animal groups. Social network approaches are useful in describing association patterns and provide promising tools for investigating the dynamics of change in social structure but have rarely been used to quantify how animal associations change over time. In this study, we describe and test a framework for temporal analysis of social structure. We propose an analytical framework of methods that integrates across social scales and comparatively analyses change in social structure across multiple types …

Comparison Of Two Models For Estimating Mortality From Baitings With Compound Drc-1339 Concentrate Avicide, Jeffrey H. Homan, Randal S. Stahl, George M. Linz

United States Department of Agriculture Wildlife Services: Staff Publications

In the U.S., DRC-1339 baitings for blackbirds (Icteridae) are generally done under the pesticide label, Compound DRC-1339 Concentrate e Staging Areas. DRC-1339 is a slow-acting avicide and gives the birds enough time to leave the baiting sites. Carcass searches and other forms of onsite counts are ineffective. Instead, linear models (LM) are used. The LM are based on esophageal analyses of several blackbird species collected while feeding at staging area bait sites. Biases and large variances can occur with this type of sampling. As an alternative to the LM, we developed a semi-mechanistic model (SM) that combined mechanistic modeling of …

The Use Of Diazacon™ To Limit Fertility By Reducing Serum Cholesterol In Female Grey Squirrels, Sciurus Carolinensis, Brenda A. Mayle, Mark Ferryman, Andrew Peace, Christi A. Yoder, Lowell A. Miller, David Cowan

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: The grey squirrel, Sciurus carolinensis Gmelin, is an invasive alien species introduced into Great Britain in the late nineteenth century and into Northern Italy during the early twentieth century. Grey squirrels have displaced the native European red squirrel, Sciurus vulgaris L., throughout much of Great Britain and have a significant impact on trees and woodlands through bark-stripping activity. In Britain, eradication is no longer an option at a regional scale, but fertility control offers a non-lethal approach to reducing negative impacts. The cholesterol mimic DiazaCon™ has been successfully used to inhibit reproduction in some species. These studies aimed to …

Laboratory Efficacy Of An Anthraquinone-Based Repellent For Reducing Bird Damage To Ripening Corn, James C. Carlson, Shelagh K. Tupper, Scott J. Werner, Susan E. Pettit, Michele M. Santer, George M. Linz

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife repellents provide a non-lethal alternative for managing agricultural impacts associated with wildlife depredation. To evaluate a potential bird repellent for ripening corn, we conducted a feeding experiment at the United States Department of Agriculture, National Wildlife Research Center with 66 red-winged blackbirds (Agelaius phoeniceus). Using a two-choice experimental design we tested the efficacy of Avipel repellent (a.i. 50% 9,10-anthraquinone) on ripening sweet corn. Red-winged blackbirds consumed an average of 8.6% ± 0.9% of kernels from untreated ears and 5.3% ± 1.1% from ears treated with anthraquinone. The interaction between anthraquinone concentration and corn treatment suggests a positive …

Experimental Infection Of White-Tailed Deer (Odocoileus Virginianus) With Northern European Bluetongue Virus Serotype 8, Barbara S. Drolet, Lindsey M. Reister, Tara D. Rigg, Pauline Nol, Brendan K. Podell, James O. Mecham, Kurt C. Vercauteren, Piet A. Van Rijn, William C. Wilson, Richard Bowen

United States Department of Agriculture Wildlife Services: Staff Publications

Bluetongue (BT) is an insect-transmitted, economically important disease of domestic and wild ruminants. Although only five of the 26 reported bluetongue virus (BTV) serotypes are considered endemic to the USA, 10 exotic serotypes have been isolated primarily in the southeastern region of the country since 1999. For an exotic BTV serotype to become endemic there must be susceptible animal species and competent vectors. In the USA, sheep and white-tailed deer (WTD) are the primary sentinel livestock and wildlife species, respectively. In 2006, BTV-8 was introduced into Northern Europe and subsequently overwintered, causing unprecedented livestock disease and mortality during the 2006– …