Digital Commons Network^™

A Multiple Input Multiple Output Framework For The Automatic Optical Fractionator-Based Cell Counting In Z-Stacks Using Deep Learning, Palak Dave

USF Tampa Graduate Theses and Dissertations

Quantifying cells in a defined region of biological tissue is critical for many clinical and preclinical studies, especially in pathology, toxicology, cancer, and behavior. Unbiased stereology is the state-of-art method for quantification of the total number and other morphometric parameters of stained objects in a defined region of biological tissue. As part of a program to develop accurate, precise, and more efficient automatic approaches for quantifying morphometric changes in biological tissue, our group has shown that both deep learning-based and hand-crafted algorithms can estimate the total number of histologically stained cells at their maximal profile of focus in extended depth …

Modeling, Control, And Operation Of A Grid-Tied Solar Photovoltaic Inverter In Unbalanced Conditions, Abdulhakim Alsaif

USF Tampa Graduate Theses and Dissertations

With the increasing penetration of inverter-based resources (IBRs) integrated into powersystem grids as well as in microgrids (MGs), operational challenges and stability issues have been identified by the industry. In the last few years, solar Photovoltaic (PV) farm plants were allowed to be disconnected (isolated from the grid) during abnormal operation conditions to not only avoid stability issues but also to protect any damage to the solar PV inverter from such overcurrent or overvoltage. Most solar PV inverters operate in a conventional grid-following (GFL) control, where unbalanced operation becomes a critical issue. This control scheme regulates only positive sequence currents. …

Bulk Glass As Compressive Reinforcement In Structural Elements, John Cotter

USF Tampa Graduate Theses and Dissertations

All composites are formulations of materials that allow for the improvement of material properties by compositing materials with differing properties. For example, straw, which has a low compression strength and stiffness but relatively high tensile stiffness, composites well with clay, which has a high compression stiffness and low tensile strength, to create a material that utilizes a clay base with straw to greatly improve the strength of clay. In general, the most economical composites are those that combine a low-cost binder, or matrix, with higher strength reinforcing materials.

Contrary to popular knowledge, bulk glass is an exceedingly strong material when …

Dynamic Study Of Inverter-Based Resources In Weak Grids, Zhengyu Wang

USF Tampa Graduate Theses and Dissertations

As the main technology behind renewable energy resources, such as solar PV and wind turbines, inverter-based generation penetration is increasing aggressively. Besides the congestion concerns when interconnecting a large-scale inverter-based resource (IBR) into the power grid, stability issues are commonly existing. The stability events occur more frequently when the resources are in remote area because the transmission line is long and grid strength is weak. One of the most representative and widely observed phenomena of IBR instability is low-frequency oscillation. And this dissertation focuses on investigating the low-frequency oscillations of IBR in weak grids.

The main objectives of the investigation …

Quantifying A 21-Year Surface Water And Groundwater Interaction In A Ridge And Valley Lake Environment Using A Highly Constrained Modeling Approach, Richard T. Bowers Jr.

USF Tampa Graduate Theses and Dissertations

Karst lakes are ubiquitous in ridge terrain settings in limestone aquifer coastal plain environments. In west-central Florida, these lakes are frequently connected to the Upper Floridan aquifer and have unique aquifer recharge characteristics. They have been selectively studied because they commonly have no or very limited surface water discharge and limited drainage areas, have appreciable surface water and groundwater interaction and leak to the deep aquifer. An innovative modeling approach was developed to collectively understand and more precisely quantify this vertical leakage, both from a lake-specific and regional water budget standpoint, for a 21-year study period (2000-2020). A unique, calibrated …

Stochastic Models For Resilience Assessment And Improvement, Weimar Ardila Rueda

USF Tampa Graduate Theses and Dissertations

Resilience refers to the ability of a system to absorb and mitigate the impact of potential disruptions and return to normal operational conditions. The above notion of resilience allows us to distinguish two stages in a system's post-disruption response, the absorptive dimension and the recovery dimension. The absorptive dimension is related to a system's robustness and capacity to mitigate initial loss posterior to a disruption. Meanwhile, the recovery dimension is the system's rapidity to return and reach an acceptable level of functionality after the disruption occurrence.

A social-physical (SP) system's post-disruption response is related to its resilience capacity so building …

Aldosterone Treatment Protects Connexin 30 And 43 Expression For Age-Related Hearing Loss, Jennifer A. Pineros Valencia

USF Tampa Graduate Theses and Dissertations

Age-related hearing loss (ARHL), also known as presbycusis, is the number one communication disorder affecting our aged population. Connexin proteins are essential for intercellular communication throughout the human body. Mutations in connexin genes have been linked to human syndromic and nonsyndromic deafness; thus, we hypothesize changes in connexin gene and protein expression with age may be involved in the development of ARHL. In our current study, connexin mRNA gene expression for CBA/CaJ mice at different age points were examined and correlations were analyzed between the changes in gene expression and functional hearing measures, such as ABRs and DPOAEs. Moreover, we …

Climate Action And Indigenous Land Relations: A Case Study In Nagaland, Northeastern India, Osensang Pongen

USF Tampa Graduate Theses and Dissertations

This research grew out of my interest in the implications of climate action, especially its effects in natural-resource-rich peripheral regions like my home state of Nagaland. This study will be of interest to anyone seeking a deeper understanding of the ways in which global ecodevelopment programs expand and mutate at the micro-scales in Indigenous communities. This includes structural transformations to their Indigenous socio-economic and political lifeworlds, their cosmologies, and their affective relations with their ancestral lands and environment. More broadly, the study draws attention to the growing challenges confronting Indigenous peoples in the Global South who are being seduced by …

Theoretical And Experimental Analyses Of Acoustofluidic Phenomena For Biosensing Applications, Pradipta Kr. Das

USF Tampa Graduate Theses and Dissertations

Acoustofluidics is a well-proven tweezer serving various applications such as particle manipulation, cell trapping and separations, fluid mixing, and bio-sensing. In this thesis, we explored several aspects of acoustofluidics such as acoustic streaming, acoustothermal heating and acoustophoretic manipulations of micron sized objects, in the context of biosensing applications.

In Chapter 2, inner acoustic streaming for second-order fluids has been studied analytically by employing asymptotic expansions for thin Stokes layer and low acoustic Mach number. In addition, a multiple-time-scale approach has been adopted to separate the primary oscillatory flow and the steady acoustic streaming. The study considers two sample cases: (i) …

L-Ergothioneine Treatment In Old Cba/Caj Male Mice Slows The Progression Of Age-Related Hearing Loss, Mark A. Bauer

USF Tampa Graduate Theses and Dissertations

The naturally occurring amino acid, L-Ergothioneine (EGT), has immense potential as a therapeutic, having shown promise in the treatment of other disease models, including neurological disorders. EGT is naturally uptaken into cells via its specific receptor, OCTN1, suggesting that it is highly conserved, to be utilized by cells as an antioxidant and anti-inflammatory, particularly under stressed conditions. In our current study, EGT was administered over a period of 6 months to 25-26 month old CBA/CaJ mice as a possible treatment for age-related hearing loss (ARHL), since presbycusis has been linked to higher levels of cochlear oxidative stress, apoptosis and chronic …

Optimizing Lake Okeechobee Watershed Management And Reservoir Operations For Water Quality Improvement, Osama Mahrous Mossad Tarabih

USF Tampa Graduate Theses and Dissertations

Rivers around the globe have been altered as people have constructed water resources infrastructure to store water for different human needs, causing negative impacts on ecosystem processes and functions. Environmental flows have been introduced to restore deteriorated riverine ecosystems; however, environmental flows have not always been practical or adequate to restore environment conditions in rivers globally. Lake Okeechobee is a large subtropical lake that plays a pivotal role in South Florida water system providing various water demands for the surrounding communities as well as providing crucial services to the ecosystem of South Florida. However, hydrologic alterations and nutrient (phosphorus and …

Corrosion Performance Of Carbon And Galvanized Steel In Calcium Silicate Cement, Maria Cardoso

USF Tampa Graduate Theses and Dissertations

The concrete manufacturing industry has faced challenges with reducing carbon dioxide emissions as it currently produces about 8% of the world’s anthropogenic carbon dioxide emissions. With global atmospheric carbon dioxide concentrations being higher than ever, the solution is urgent. Due to this, a more sustainable type of concrete has been proposed.Calcium silicate cement (CSC) composed of essentially the same raw materials as ordinary portland cement (OPC) cures through the consumption of carbon dioxide rather than hydration like OPC. CSC is being explored as a potential alternative to OPC, however, there is little to no information on its corrosion related durability. …

Structural And Biochemical Characterization Of Epigenetic And Fusion Tyrosine Kinase Cancer Targets For Drug Development, Melissa Bikowitz

USF Tampa Graduate Theses and Dissertations

Cancer is a significant global health burden, as ~40% of the human population is diagnosed with cancer over a lifetime, half of which succumb to the disease. To develop novel compounds as potential cancer therapeutics, an in-depth structural and biochemical understanding of proteins involved in oncogenesis is key. This dissertation centers around the comprehensive structural and biochemical characterization of two classes of proteins critical for cancer cell survival: bromodomain-containing proteins (BRDs) and fusion tyrosine kinases (FTKs).In 2010, small molecule inhibitors targeting bromodomains (epigenetic reader proteins) became heavily popularized due to the druggability and potent anticancer effects of BET inhibitors. …

Orthogonal Saw Biosensor Fem Simulation Based On St-Quartz With Crystalline Zno Guiding Layer, Saurabh Patwardhan

USF Tampa Graduate Theses and Dissertations

Detection of trace amounts of biomarkers is a critical requirement in sensing the presence of conditions such as early-stage cancer. The ST cut of the Quartz crystal (ST-Quartz) supports two fundamental surface acoustic waves (SAWs) in mutually perpendicular, or orthogonal, directions: the Rayleigh wave and the shear-horizontal wave (RSAW and SH-SAW, respectively). The orthogonality enables the use of ST-Quartz in biofouling removal by the RSAW and mass sensing via the SH-SAW simultaneously. The purpose of this study is to find an ideal guiding layer and wavelength configuration that can enhance the sensing capability without sacrificing the biofouling removal ability for …

Community Heritage: A Source Of Health/Medical Aspirational Capital For Black Males, Lafrance Clarke Jr.

USF Tampa Graduate Theses and Dissertations

There is a recognized need for more Black male healers who pursue careers in medical fields.Thus, the purpose of this study was twofold: to explore historical documents to tell the histories of men of African descent who practiced healing in Florida and, from those histories, create a virtual heritage tour to support teaching about leadership through African American history and narratives of healing. The research questions were: How does the heritage curriculum history about Black men healers of FL provide a historical counter-narrative about Black men and their leadership in connection to health/medicine?How do historical counter-narratives about Black men support …

An Exploration Of Accelerated Student Perspectives On Participation Experiences And Making Career Choices, Jennifer Meier

USF Tampa Graduate Theses and Dissertations

Acceleration program options have grown rapidly in Florida and have been used to help students pursue an associate or bachelor’s degree. The purpose of this qualitative study was to explore the experiences that former dual enrolled students had with selecting a major and a career. A snowball sampling approach was used to identify ten participants from the southwestern region of Florida with 12 or more credit hours from dual enrollment. Social Cognitive Career Theory (SCCT) was the conceptual framework used to help guide this study. The instrumentation for data collection included a short survey followed by a semi-structured interview. The …

Low-Pressure Plasma Surface Treatment Of Polycarbonate And Polyacrylic Plates With Different Plasmas And Their Characterization, Suleyman Gorkem Gizer

USF Tampa Graduate Theses and Dissertations

In this thesis, polycarbonate (PC) and acrylic (PMMA) surfaces were treated with carbon tetrafluoride (CF4), oxygen (O2), and air plasmas with a low-pressure plasma method in order to change their surface properties such as surface free energy and wettability. Moreover, different pattern sized, line, triangular and square shaped surface patterns were used to determine the shape and size-dependent changes. The characteristics of untreated and plasma treated surfaces were examined via contact angle (CA) measurements with distilled (DI) water, against plasma treatment number. Moreover, surface free energy (SFE) calculations of the PC and PMMA surfaces were carried out from sessile drop …

Plasma Treatment Of Polyethylene And Polydimethylsiloxane By Air, Oxygen, And Carbon Tetrafluoride And Their Characterization, Osman Polat

USF Tampa Graduate Theses and Dissertations

In this thesis, low density polyethylene (LDPE) and polydimethylsiloxane (silicone/PDMS) were treated with oxygen (O2) , air and carbon tetrafluoride (CF4) low pressure 13.56 MHz radiofrequency plasma. An experimental setup was used to determine process power and irradiation time for improved modification, the validation was done by measuring the contact angle of the treated samples immediately after treatment. The modification used commercially available sheets to imitate its use in the industry and various applications. The treatment aims to increase/decrease the surface free energy, making it possible to modify the intrinsic inert materials without affecting the bulk. Increased wettability, adhesion, and …

Interaction Of Sequentially Applied Interventions For Gait Symmetry, Adila Hoque

USF Tampa Graduate Theses and Dissertations

More than 50% of stroke survivors over 65 years of age experience long-term mobilityloss [50]. Hemiparesis is a form of disability that arises from this, causing paralysis on one side of the body. The long-term effects of hemiparesis include loss of locomotion, muscle spasticity, and excessive use of muscles on the non-paretic side. This leads to a gait pattern that is kinematically and kinetically asymmetric.

Several rehabilitation techniques currently exist that aim to minimize this asymmetric gait, including split-belt treadmill training, rhythmic cueing (auditory or tactile), adding mass to one foot. Each form of treatment comes with its unique strengths …

Bridging The Gap Between Academia And Industry: A Program Evaluation On University-Industry Collaboration, Lan Reneé Amboy-Biller

USF Tampa Graduate Theses and Dissertations

A significant amount of money is spent on professionalizing the workforce. At the same time, there is limited research on the effectiveness of academic-to-industry corporate partnerships as an innovative solution for meeting workforce development objectives. This exploratory study evaluates an existing University of South Florida industry partnership to identify characteristics of a sustainable university-to-industry model. The program has operated over the last nine years, providing degree completion programs for industry employees. This program evaluation includes an analysis of stakeholders, advisors, mentors, and participants’ perceptions. The evaluation findings identified key factors of consideration required when establishing and operating a University-Industry partnership. …

Designing A Messaging Strategy To Improve Information Security Policy Compliance, Federico Giovannetti

USF Tampa Graduate Theses and Dissertations

Lack of employee compliance with information security policies is a key factor driving security incidents. Information security practitioners struggle to enforce policy compliance while employees try to curtail safeguards in favor of expediency and other perceived business goals. Several studies have shown individual and organizational factors influencing this type of employee behavior. However, few have recommended management-level interventions that can be used as a solution framework by information security practitioners.

This research utilized the Design Science Research (DSR) methodology to develop a management-level intervention based on a messaging strategy that aims to help information security practitioners improve the information security …

Development Of An Automated Platform For Sensing And Differentiating Vapor-Phase Btex Constituents, Jonathan Samuelson

USF Tampa Graduate Theses and Dissertations

Light aromatic hydrocarbons are an inevitable byproduct of fossil fuel extraction, refinement, distribution, and use. The four lightest and most prevalent of these are benzene, toluene, ethylbenzene, and xylene, which are known collectively as BTEX. In spite of their chemical similarity these species have markedly different effects on human health and substantially different concentrations are permitted by OSHA in workplaces and by the EPA in ambient air and groundwater. Real-time detection, identification, and quantification of these species is therefore of great importance wherever they see industrial use.This work represents the continuation and advancement of a line of research in which …

An Anthropology With Human Waste Management: Non-Humans, The State, And Matters Of Care On The Placencia Peninsula, Belize, William Alex Webb

USF Tampa Graduate Theses and Dissertations

The management of human waste is a seldom studied phenomenon in anthropology. Yet across the globe, in countries both rich and poor, it presents pervasive and difficult to tame problems. This dissertation draws on complimentary theories of management and entanglements to explore the practices and processes of organizing human waste on the Placencia Peninsula, Belize. The results illustrate how problems are conditioned and defined by messy relations between institutions, people, technologies, materials, and ecological life.

Fieldwork and analysis for this work was a culmination of years of interdisciplinary collaboration between other anthropologists and engineers at the University of South Florida. …

The Biological Roles Of Tau Protein In The Vasculature And The Regulation Of Vegfr1 Signaling By Heparan Sulfate, Yanan Zhu

USF Tampa Graduate Theses and Dissertations

My dissertation consists of two research projects. The first project investigated the roles of extracellular tau in the vasculature, including tau-induced angiogenesis and tau internalization, a topic related to tauopathy. The second project was to understand the regulatory functions of heparan sulfate proteoglycans in VEGFR1 signaling.

Tauopathies are a class of neurodegenerative diseases, including Alzheimer's disease, and are characterized by the appearance of intraneuronal tau inclusion in the brain and the patient's cognitive decline. Recent studies have emerged that soluble and aggregated tau also exists outside neurons in the central nervous system. Intriguingly, overexpressing tau in neurons in the mouse …

Statistical Methods For Reliability Test Planning And Data Analysis, Oluwaseun Elizabeth Otunuga

USF Tampa Graduate Theses and Dissertations

This dissertation develops several statistical methods to advance the techniques and applications in the fields of reliability test planning and data analysis as well as statistical modeling and analysis in survival analysis.

The first project focuses on developing new demonstration test plans for lifetime data based on considering multiple objectives. Reliability demonstration tests have been broadly used for assuring reliability performance at the desired confidence level. We consider lifetime data that follows a Weibull distribution which has been broadly used for modeling a variety of shapes of lifetime distributions. When planning a demonstration test, there are often multiple aspects to …

Task Progress Assessment And Monitoring Using Self-Supervised Learning, Sainath Reddy Bobbala

USF Tampa Graduate Theses and Dissertations

Robotic manipulation for cooking requires a thorough understanding of the cooking environment. The robot must understand the cooking objects and their states at each intermediate level as the process continues. To understand these states, we need frame-level annotations. To overcome this frame-level dependency, we introduce a self-supervised learning method to obtain the frame-level state representation with ”temporal video alignment” and ”contrastive learning.”In this work, we use self-supervised learning to train a model using multiple videos of the same action being performed in various settings. This model can extract frame-level embedding space and align videos via simple distance-based matching. We show …

Statistical Models Of Traffic Injury Severities: The Effects Of Driver Nationality And The Time-Of-Day On Pedestrian Injuries, Asim Alogaili

USF Tampa Graduate Theses and Dissertations

Roadway accidents have long been a major cause of casualties among all roadway users including drivers and pedestrians. Researchers have used various advanced methodologies to precisely identify affecting factors to help policy makers implement safety measures that effectively mitigate such losses. For a variety of reasons such factors, especially those related to roadway users, are subject to constant changes over time. This work investigates, in depth, the effect of multiple variables on the injury severity levels of both drivers and pedestrians using the most recent and advanced methodological approaches.

First, the dissertation starts by investigating factors that significantly contribute to …

Towards More Task-Generalized And Explainable Ai Through Psychometrics, Alec Braynen

USF Tampa Graduate Theses and Dissertations

In this work, we propose that adopting the methods, principles, and guidelines of the field of psychometrics can help the Artificial Intelligence (AI) community to build more task-generalizable and explainable AI. Three arguments are presented and explored. These arguments are that psychometrics can help by providing 1) a framework for formulating better datasets, 2) psychometric AI data that can lead to models of generalization in AI, and 3) explainable AI through more informative evaluations.

A review of psychometrics and psychological generalization is performed, along with an overview of evaluation, generalization, and explainability in AI. Various ideas are presented throughout for …

Comparison Study Of Consumer’S Perception Toward Urban Air Mobility In The United States And Rest Of The World Using Social Media Information, S M Toki Tahmid

USF Tampa Graduate Theses and Dissertations

Urban Air Mobility (UAM) is a wide concept that will enable access to on-demand air mobility, cargo delivery, medical applications, and emergency services that is run through a connected and integrated transportation system. This study aims to investigate social media data to compare the major topics discussed in the United States and rest of the world and perform a sentiment analysis to see if the comments are positive, negative, or neutral. Twitter was the primary source of information that was used in this thesis, as it is a place where people interact, create, and share information and ideas online. In …

Generative Spatio-Temporal And Multimodal Analysis Of Neonatal Pain, Md Sirajus Salekin

USF Tampa Graduate Theses and Dissertations

Neonates can not express their pain like an adult person. Due to the lacking of proper muscle growth and inability to express non-verbally, it is difficult to understand their emotional status. In addition, if the neonates are under any treatment or left monitored after any major surgeries (post-operative), it is more difficult to understand their pain due to the side effect of medications and the caring system (i.e. intubated, masked face, covered body with blanket, etc.). In a clinical environment, usually, bedside nurses routinely observe the neonate and measure the pain status following any standard clinical pain scale. But current …