Digital Commons Network^™

The Use Of Electrical Charge To Produce Cell-Cell Contact Prior To Electrofusion, Jyothi Fernandes

USF Tampa Graduate Theses and Dissertations

From previous studies it has been demonstrated that the fusion of tumor cells with antigen-presenting cells generates hybrids that are known to induce anti-tumor immunity. With the advancement of scientific research and medicine, the need to produce cell-cell hybrids for cancer immunotherapy and for various other applications is substantial.

Among the many methods used to generate these hybrid cells, electrofusion is a technique that is more widely used and recognized as a method to efficiently produce hybrids. Electrofusion requires two steps. In the first step, cells are brought into close adjacent contact either by a mechanical method like centrifugation or …

Aortic Valve Analysis And Area Prediction Using Bayesian Modeling, Miheer S. Ghotikar

USF Tampa Graduate Theses and Dissertations

Aortic valve stenosis affects approximately 5 out of every 10,000 people in the United States. [3] This disorder causes decrease in the aortic valve opening area increasing resistance to blood flow. Detection of early stages of valve malfunction is an important area of research to enable new treatments and develop strategies in order to delay degenerative progression. Analysis of relationship between valve properties and hemodynamic factors is critical to develop and validate these strategies.

Porcine aortic valves are anatomically analogous to human aortic valves. Fixation agents modify the valves in such a manner to mimic increased leaflet stiffness due to …

Full-Page Versus Partial-Page Screen Designs In Web-Based Training: Their Effects On Learner Satisfaction And Performance, Phillip Eulon Grace

USF Tampa Graduate Theses and Dissertations

This is a report on research regarding the screen layout of Web-based training (WBT) programs, conducted with an eye toward providing evidence-based guidance for the design and development of WBT interfaces. Specifically, the study investigated the relative instructional benefits of two general types of WBT screen design, full-page and partial-page, in terms of both learner performance and learner satisfaction. The main hypotheses of the study were that the full-page design option would yield significantly better outcomes in both categories of interest.

The study employed a mixed-method design, generating both quantitative and qualitative data. The main phase of the study was …

Reducing Pollutants In Industrial Stormwater Runoff: Improved Water Quality Protection Using Prioritized Facility Regulation, Lindsay M. Griffen

USF Tampa Graduate Theses and Dissertations

Stormwater pollutants originating from industrial facilities can lead to degraded water quality, even in residentially dominated regions of the country. The National Pollutant Discharge Elimination System permit program regulates stormwater pollutants generated at industrial sites using Multi-Sector General Permits (Generic permits) for industrial facilities and a permit requirement for Municipal Separate Storm Sewer System (MS4) operators. All industrial facilities within 11 broad categories of industry are responsible for self-identifying the need to comply with the Generic permit, and subsequently, implementing self-selected pollution prevention strategies. MS4 operators are required to identify and inspect “high risk” industrial and commercial facilities that may …

Development Of A Dna Vaccine Against Streptococcus Mutans: A Novel Approach To Immunization Against Dental Caries, Thomas Han

USF Tampa Graduate Theses and Dissertations

Streptococcus mutans is the main causative agent of dental caries, which is a widespread infectious disease. A number of surface molecules are involved in the pathogenicity of this organism, including adherence and aggregation factors. The wallassociated protein A (WapA) of Streptococcus mutans GS-5 was previously demonstrated to be a sucrose-dependent adherence and aggregation factor, and is a larger precursor to extracellular antigen A (AgA), a candidate antigen for a dental caries vaccine.

The full-length wapA gene and a C-terminal truncated version agA encoding the AgA were cloned into the mammalian expression vector pcDNA 3.1/V5/His-TOPO. The above constructs were …

A Discourse Concerning Two New Compositions, Stanley D. Harrison

USF Tampa Graduate Theses and Dissertations

This project addresses problems for theorists of writing and composition that arose in the 1990s when capital privatizes the production of internetworked writing and starts operating in the manner of a practicing compositionist. I begin by noting that capital in the 1990s converted internetworked writing machines into fixed capital and started composing its version of the cultural form of the "social" we call the Internet. Thereafter, I argue that composition theorists can best understand the Internet, internetworked writing, and internetworked subjectivities if they regard capital as a formidable compositionist, one capable of making the machinofactured internetworked composition into a privately …

The Effects Of An Internet-Based Program On The Early Reading And Oral Language Skills Of At-Risk Preschool Students And Their Teachers’ Perceptions Of The Program, Mary Huffstetter

USF Tampa Graduate Theses and Dissertations

This investigation examined the effects of instruction, within the context of the Headsprout Reading Basics program, on the oral language and early reading skills of atrisk preschool students, and their teachers’ perceptions of the program. Random assignment was used in a pretest-posttest, control group design to assess the effects of this program. Thirty-one students, across two preschool settings, participated in the experimental group, and 31 students participated in the comparison group. The experimental group received instruction through the Headsprout Reading Basics program, which teaches the alphabetic principle, decoding strategies, print awareness, vocabulary, and deriving meaning from texts. The comparison group …

The Effects Of Small-Scale Heterogeneities On Aquifer Storage Recovery Systems, William C. Hutchings

USF Tampa Graduate Theses and Dissertations

Aquifer Storage Recovery (ASR) is a recently developed (circa 1970) method (in the U.S.A.) to reduce groundwater-pumping stresses by injecting treated wastewater or surface water during periods of low demand into an aquifer followed by its recovery during periods of high demand. This method has been successfully implemented in numerous locations across the U.S.A. and worldwide, mainly due to the cost savings provided by the use of an unlimited reservoir (aquifer) in which to store water compared to the costs to construct surface impoundments and the inherent problems with storing such water for extended periods of time under evaporative atmospheric …

A Comparison Of Traditional Physical Laboratory And Computer Simulated Laboratory Experiences In Relation To Engineering Undergraduate Students’ Conceptual Understandings Of A Communication Systems Topic, Giti Javidi

USF Tampa Graduate Theses and Dissertations

This study was designed to investigate an alternative to the use of traditional physical laboratory activities in a communication systems course. Specifically, this study examined whether as an alternative, computer simulation is as effective as physical laboratory activities in teaching college-level electronics engineering education students about the concepts of signal transmission, modulation and demodulation. Eighty undergraduate engineering students participated in the study, which was conducted at a southeastern four-year university.

The students were randomly assigned to two groups. The groups were compared on understanding the concepts, remembering the concepts, completion time of the lab experiments and perception toward the laboratory …

Shape Based Stereovision Assistance In Rehabilitation Robotics, Michael Ulrich Jurczyk

USF Tampa Graduate Theses and Dissertations

A graphical user interface program was created along with shape models, which allow persons with disabilities to set up a stereovision system with off-theshelf hardware and detect objects of interest, which can be picked up using a sensor assisted telerobotic manipulator.

A Hitachi KP-D50 CCD camera and an Imaging Source CCD camera were used along with two Imaging Source DFG/LC1 frame grabbers to set up a stereovision system. In order to use the stereovision system, the two main problems of correspondence and reconstruction are solved using subroutines of the program created for this work.

The user interface allows the user …

Opportunistic Maintenance Policy Of A Multi-Unit System Under Transient State, Sulabh Jain

USF Tampa Graduate Theses and Dissertations

Most modern systems are equipped with very complex, expensive, and high technology components whose maintenance costs have become an increasingly large portion of the total operating cost of these systems. Thus, the efficacy of the maintenance policy for these and related systems has become a major concern to both manufacturing and design engineers. Different kinds of maintenance strategies have been proposed to solve the problem. While some of these have proven effective, there is yet no definitive approach that has been found that support the maintainability requirements of transient systems or systems that exhibit transient behavior. Transient behavior is the …

A Generalized Decision Model For Naval Weapon Procurement: Multi-Attribute Decision Making, Jin O. Chang

USF Tampa Graduate Theses and Dissertations

For any given reason, every year many countries spend a lot of money purchasing at least one weapon. Due to the secret character of the military, the decision process for specific weapon procurement is shrouded. Moreover, there are several funds loss cases due to mistakes in weapon contractions. Weapon procurement requires very large amounts of money which comes from tax payers. Therefore, an effort to reduce a possible monetary loss is needed.

A decision process based on an analytic model can present a better chance to decision makers for better weapon decisions. In general, weapon procurement decision is a multi …

A Geographic Information System For Dynamic Ridematching, Sasha Dos-Santos

USF Tampa Graduate Theses and Dissertations

The Online Transportation Option System (OTOS) is a Geographic Information System (GIS) that addresses many of the limitations associated with traditional dynamic ridematching applications. The main improvements are in the areas of trip scheduling and match searching. OTOS is unique in its ability to accept trips with schedules that can not be expressed in terms of a regular weekly trip. OTOS also distinguishes itself in its use of spatial analysis techniques to locate matches. Specifically, the use of a shortest path solver enables the ridematching algorithm to perform a search along the path of a user’s trip, in addition to …

Re-Engineering Graduate Medical Education: An Analysis Of The Contribution Of Residents To Teaching Hospitals Utilizing A Model Of An Internal Medicine Residency Program, Ian M. Elius

USF Tampa Graduate Theses and Dissertations

According to the Institute of Medicine (IOM), the U.S. health care delivery system does not provide consistent, high-quality medical care to all people all the time. As a significant component of the health care delivery system, the state of Graduate Medical Education in the United States has prompted much analysis in recent years due to the general view that desired and actua l outcomes are increasingly at variance with each other. One area of focus has been the implications of change for provider credentialing and funding of graduate medical education.

With this research we test the hypothesis that residents perform …

An Integrated Optimal Design Method For Utility Power Distribution Systems, Ralph E. Fehr

USF Tampa Graduate Theses and Dissertations

This dissertation presents a comprehensive and integrated design methodology to optimize both the electrical and the economic performance of a utility power distribution system. The proposal is structured to facilitate its adoption and incorporation into the existing utility infrastructure by allowing the various portions of the new design to be implemented gradually into the existing infrastructure without the need to abandon the portions of the existing system that are performing satisfactorily.

The topology of the substation plays a vital role in determining both the reliability and the economy of the distribution system. The ring bus topology is offered as the …

The Analysis Of Dielectric Loss In Co-Planar Waveguide Structures Using Generalized Transverse Resonance, James William Culver

USF Tampa Graduate Theses and Dissertations

There are several methods for the full-wave characterization of waveguide discontinuities; Finite Element Method (FEM), Finite Difference Technique (FDT), and Method of Moments (MoM) are popular. However, these methods are not easily applied when studying the ‘modal anatomy’ of a discontinuity. Other full-wave techniques are better suited. This dissertation discusses the formulation of a technique known as Generalized Transverse Resonance (GTR), which is a subset of Method of Moments. Generalized Transverse Resonance is a hybrid method combining the Transverse Resonance Method (TRM) with the Mode Matching Technique (MMT).

The understanding of the generalized transverse resonance method starts with a discussion …

Development Of Enhanced Multiport Network Analyzer Calibrations Using Non-Ideal Standards, John Edward Daniel

USF Tampa Graduate Theses and Dissertations

An Improved Short-Open-Load-Reciprocal (SOLR) Vector Network Analyzer (VNA) calibration is developed and validated. Through the use of a more complex load model the usable frequency range of the SOLR calibration algorithm is expanded. Comparisons are made between this new calibration and existing calibration techniques that are known to be accurate at high frequencies. The Anritsu 37xxx Lightning series 65GHz VNA is used as the principle measurement tool for calibration comparison and verification. This work is built off of previous work done at USF in which it is shown that the Short-Open-Load-Thru (SOLT) calibration’s accuracy improves through the implementation of more …

Evaluation Of Available Bandwidth Estimation Tools (Abets) And Their Application In Improving Tcp Performance, Yegyalakshmi Easwaran

USF Tampa Graduate Theses and Dissertations

Available bandwidth is a time-dependant variable that defines the spare bandwidth in an end-toend network path. Currently, there is significant focus in the research community on the design and development of Available Bandwidth Estimation Tools (ABETs), and a few tools have resulted from this research. However, there is no comprehensive evaluation of these tools and the research work in this thesis attempts to fill that gap. A performance evaluation of important ABETs like Pathload, IGI and pathChirp in terms of their accuracy, convergence time and intrusiveness is conducted in several scenarios. A 2 k factorial design is carried out to …

Automatically Determining Route And Mode Of Transport Using A Gps Enabled Phone, Himanshu Gilani

USF Tampa Graduate Theses and Dissertations

A system consisting of a GPS-enabled phone and a database has been designed and implemented. This system is capable of recording the route traveled by a user and determining the mode of transport (walk, bicycle, car or bus) used. The Java code running in the GPS-enabled phone automatically records location data, determines critical locations for the trip, and transmits the locations to a central database using the wireless capabilities of the phone. As the route information arrives at the database, it is processed by the mode detection algorithm that determines the mode of transportation being used by the individual for …

Reconfigurable Wireless Sensor Platform For Training And Research In Networked Embedded Systems, Oscar V. Gonzalez

USF Tampa Graduate Theses and Dissertations

Ever since the University of California, Berkeley released the first commercial Wireless Sensor Network, (WSN), "mote", applications that employ the WSN technology have increased many fold. There are many kinds of prototypes and architectures for WSNs that are being developed by major communication companies. Due to the lack of a common standard for different physical and MAC layer protocols, interoperability among the wireless systems is constrained. Additionally, remote sensing capability, reconfigurability and interoperability have not yet been designed. In the current platforms hardware upgrading has to be performed on-site by replacing the old sensors with new ones. This procedure increases …

Relationship Of Waste Characteristics To The Formation Of Mineral Deposits In Leachate Collection Systems, Antonio J. Cardoso

USF Tampa Graduate Theses and Dissertations

Landfill leachate is generated as a result of reactions between water percolating through the landfill and wastes. Under normal conditions leachate is found at the bottom of landfills and from there, its movement can be controlled with collection systems to be treated, discharged, or recirculated. Landfill leachate collection systems are positioned above the liner and are designed to collect liquid under gravitational flow for the entire active, closure, and post-closure periods. Clogging of any portion of the system can lead to higher hydraulic heads and increase the potential for leakage through the liner.

To reduce the quantity of municipal solid …

Mapping The Major Axis Of Tephra Dispersion With A Mesoscale Atmospheric Model: Cerro Negro Volcano, Nicaragua, Marc A. Byrne

USF Tampa Graduate Theses and Dissertations

Models of tephra fallout are used to assess volcanic hazards in advance of eruptions and in near-real-time. Current models often approximate the wind field using simplistic assumptions of the atmosphere that cannot account for typical variations in wind velocity that occur in time and three-dimensional space. Here, a widely used mesoscale atmospheric model is used to improve forecasts of the location of the major axis of dispersion for erupting plumes. The Pennsylvania State University-National Center for Atmospheric Research fifth-generation Mesoscale Model (MM5) specializes in atmospheric prediction for regions on the order of ten to hundreds of kilometers on a side. …

Sound Localization Abilities Of Two Florida Manatees, Trichechus Manatus Latirostris, Debborah E. Colbert

USF Tampa Graduate Theses and Dissertations

Florida manatees (Trichechus manatus latirostris) live in the shallow, often turbid inland and coastal waters of the southeastern United States. Since their vision is poor (Bauer et al., 2003), other senses probably guide orientation. Previous studies have found that manatees can hear over 40 kHz (Gerstein et al., 1999) and have the capacity for rapid auditory temporal processing (Mann et al., 2005). However, it is not known if manatees have the ability to localize underwater sounds.

Two Florida manatees were trained to identify underwater sound source locations using a four-choice discrimination paradigm. Three broad-band signals ( 0.2 - …

Self-Esteem And Academic Achievement: A Comparative Study Of African American Students In A Traditional Public School And A Magnet School, Sheresa L. T Fairclough

USF Tampa Graduate Theses and Dissertations

This study evaluated whether differences existed in the academic achievement and self-esteem of African American students in a traditional public school and a choice school. The choice school selected was a technology-focused magnet school. All of the participants were in grades 3-5 and were administered the Florida Comprehensive Assessment Test (FCAT) during the 2003-2004 school year. The FCAT was used to assess academic achievement in reading and math while the Culture Free Self-Esteem Inventories ñ Third Edition (CFSEI-3) was used to assess their self-esteem. Demographic information was also collected to identify factors other than school type that contribute to differences …

Toward Predicting Barrier Island Vulnerability: Simple Models For Dune Erosion, Laura A. Fauver

USF Tampa Graduate Theses and Dissertations

The objective of this study is to quantify the accuracy of two engineering models for dune erosion (SBEACH and EDUNE), and to determine which of the two models is best suited for predicting barrier island vulnerability due to extreme storm events. The first model, SBEACH, computes sediment transport using empirically derived equations from two large wave tank experiments. The second model, EDUNE, theoretically relates excess wave energy dissipation in the surf zone to sediment transport. The first mechanism for model comparison is sensitivity testing, which describes the response of the model to empirical, physical, and hydrodynamic variables. Through sensitivity tests, …

The Invisible Handshake: Interpreting The Job-Seeking Communication Of Foreign-Born Chinese In The U.S., Hongmei Gao

USF Tampa Graduate Theses and Dissertations

Building upon Granovetter’s well-known study of the job search behaviors of white males, this research extended the degree to which his findings apply across cultures to Chinese minorities, and across time to the Internet age. Using quantitative and qualitative data collected through systematic observation, questionnaire surveys, and in-depth interviews, this research investigated the impact of culture, Internet usage, gender and age on the communication patterns of foreign-born Chinese jobseekers in the U.S.

It is found that jobseekers adopt either one or a combination of traditional (printed publications and direct application), institutional (the Internet, job fairs, and employment agencies), and personal …

Maximizing The Benefits Of Its Deployments Based On Idas Performance Measures And B/C Ratios, Cuthbert R. Gibson

USF Tampa Graduate Theses and Dissertations

Traditional transportation planning models are not very sensitive to many of the benefits derived from Intelligent Transportation Systems (ITS) technologies. With the recent availability of ITS Deployment Analysis System (IDAS), there is an opportunity to conduct detailed cost-benefit analyses of ITS Alternatives. IDAS is able to estimate the impacts, benefits, and costs resulting from the deployment of many ITS components. This is accomplished by comparing the average cost of deploying and integrating the appropriate technologies, to the estimated benefits experienced by the network. The ratio of average benefits to average costs is termed the B/C ratios. While the use of …

Growth And Characterization Of Diamond And Diamond Like Carbon Films With Interlayer, Roja Gottimukkala

USF Tampa Graduate Theses and Dissertations

Diamond and diamond-like carbon films, with their exceptionally good mechanical, chemical, and optical properties, are the best materials as protective hard coatings for electronic devices and cutting tools. The biocompatibility of these materials makes it suitable for bone implants. The wide range applications of these films are hindered because of the high compressive stresses developed during the deposition. Use of carbide and nitride interfacial layers has emerged as one of the methods to reduce the compressive stresses.

The present research focuses on the study of different materials as the interfacial layers for diamond and tetrahedral amorphous carbon films. For tetrahedral …

Systematic Study Of Amyloid Beta Peptide Conformations: Implications For Alzheimer’S Disease, Jeffy Pilar Jimenez

USF Tampa Graduate Theses and Dissertations

The amyloid beta peptide particularly the 40 and 42 amino acid residues are the responsible for plaque formation in Alzheimer's disease (AD) patients. Extra cellular plaque formation has been recognized after incessant investigations along with the formation of intracellular tau protein tangles as the hallmarks of AD. Furthermore, the plaque formation has been linked mostly as a cause of the disease and the tangles mostly as a consequence. Our investigation is focused on studying the formation of AD plaques. The amyloid beta (Aß) is a physiological peptide secreted from neurons under normal conditions, along with other soluble forms cleaved from …

Simulating The Electric Field Mediated Motion Of Ions And Molecules In Diverse Matrices, Joseph Hickey

USF Tampa Graduate Theses and Dissertations

Electroporation is a methodology for the introduction of drugs and genes into cells. This technique works by reducing the exclusionary nature of the cell membrane [125, 129, 186, 189]. Electroporation has successfully been used in electrochemotherapy and electrogenetherapy [57, 68, 86, 87, 110, 112, 131]. The two major components of electroporation are an induced transmembrane potential and the motion of the deliverable through a compromised cell membrane into the target cell [38, 55, 62, 114, 131]. These two components are both dependent on the electrophoretic motion of charged species in an applied electric field [45, 64, 75, 77, 177].

Currently, …