Digital Commons Network^™

Engineering Fsh-Cer T Cells To Enhance Response To Epithelial Ovarian Cancer, Nolan J. Beatty

USF Tampa Graduate Theses and Dissertations

Ovarian cancer remains a significant cause of cancer-related mortality, with epithelial ovarian cancer (EOC) being the most common subtype. Despite advances in treatment, the 5-year survival rate for late-stage EOC remains low due to factors such as tumor heterogeneity and an immunosuppressive tumor microenvironment (TME). This study investigates the therapeutic potential of chimeric endocrine receptor (CER) T cells engineered to express follicle stimulating hormone (FSH) in a syngeneic mouse model of EOC expressing follicle stimulating hormone receptor (ID8-FSHR). We compared two different co-stimulatory domains—CD28ζ and 4-1BBζ—in FSH-CER T cells and found that FSH-CD28ζ CER T cells exhibited enhanced cytotoxicity, proliferation, …

From Shortage To Strategy: Optimizing Scarce Resource Allocation In Healthcare, Daniela Duran Cantarino

USF Tampa Graduate Theses and Dissertations

The allocation of scarce medical resources presents one of the most consequential challenges in health care, requiring decisions that balance equity and efficiency under pervasive uncertainty. This dissertation develops optimization-based frameworks for two critical domains, organ transplantation and emergency medical services, and demonstrates how operations research methods can improve upon current practice along both dimensions simultaneously.

In the domain of deceased-donor kidney allocation, two complementary frameworks are proposed. The first is a bi-objective stochastic optimization model that jointly maximizes post-transplant survival (efficiency) and prioritizes candidates with the highest pre-transplant mortality risk (equity), subject to regional chance constraints on graft failure …

Nanoscale Morphological Perturbations Of Lipid Membranes Induced By Membrane Active Peptides, Yasith Sandeepa Indigahawela Gamage

USF Tampa Graduate Theses and Dissertations

Membrane-active peptides (MAPs) are a diverse group of biologically relevant molecules that induce nanoscale modulations during their interactions with cell membranes. Understanding the precise mechanism by which these MAPs perturb or modulate the cell membranes is a cornerstone of membrane biophysics, as it facilitates rational design of useful therapeutics. That is, if we have the knowledge to determine what the peptide can do, how selective it is, or how toxic it might be, we can optimize them and design new peptide therapeutics such as for antimicrobial, anticancer, or drug-delivery applications. In general, MAPs can be categorized into two classes based …

The Isotopic Profile Of Plague Victims Buried On The Quarantine Islands Of Venice, Italy, Katherine L. Dunning

USF Tampa Graduate Theses and Dissertations

This thesis combined carbon, nitrogen, and oxygen stable isotope analysis and historical documentation to explore diet and mobility for 45 individuals whose remains were excavated from the Venetian island Lazzaretto Vecchio, where plague victims were quarantined, cared for, and buried between 1423 and 1700 CE. δ13C from bone collagen, bone apatite, and tooth enamel apatite was evaluated to determine terrestrial, aquatic, C3, and C4 contributors to diet. δ15N from bone collagen was evaluated to understand the individuals’ trophic levels. δ18O from tooth enamel apatite and bone apatite was evaluated to detect mobility over the life course. Adult diet was primarily …

Toward Intelligent Machines: Conscious Learning And Hardware-Accelerated Ai, Pavia Bera

USF Tampa Graduate Theses and Dissertations

Artificial intelligence systems excel at narrow, well-defined tasks but remain brittle at theboundaries of their training distributions: they cannot quantify uncertainty, adapt continuously to non-stationary data, or operate efficiently on energy-constrained hardware. This dissertation addresses these limitations through four coordinated contributions spanning probabilistic learning algorithms, biologically inspired temporal memory, cross-entity warning propagation via distributed associative memory, and spintronic processing-in-memory.

The first contribution introduces Boosted Bayesian Neural Networks (BBNNs), which extend standard mean-field variational inference by iteratively constructing a mixture posterior through Boosting Variational Inference. On five clinical medical classification datasets, BBNNs achieve superior uncertainty calibration—lower Negative Log-Likelihood and Expected Calibration …

Autoencoders As Classifiers Trained On Single Sources Of Radiographic Images For Generalizability Across Unseen Sources, Ryan M. Putney

USF Tampa Graduate Theses and Dissertations

Artificial neural networks trained to classify X-ray images according to disease state will learn to distinguish between technical or procedural variations in the images rather than features relevant to the disease. For example, the model might learn to recognize the machine that captured the image or to distinguish an image taken while the patient is lying supine or standing upright. The present work is aimed at using autoencoders as a generalizable classifier to detect COVID vs pneumonia (PNA) X-ray images. The first experiment was to find an architecture for a fully convolutional autoencoder (CAE) and for a convolutional autoencoder with …

Examining The Impact Of A Positive Psychology Service-Learning Experience On Undergraduates’ Mental Health And Career Intentions, Cydney E. Williams

USF Tampa Graduate Theses and Dissertations

Universities currently face a growing public health concern regarding college students’ mental health and well-being (Hobbs et al., 2022; Sheldon et al., 2021). Given this, research has explored experiences that are advantageous for students’ overall well-being, including courses on positive psychology and service-learning experiences that may promote flourishing (Hobbs et al., 2022; Maples et al., 2022). Service-learning is also well situated to support undergraduate career development (Collison & Eck, 2022). Kranzler et al. (2011) described how the pedagogical approach of service-learning aligns well with positive psychology, leading them to implement a positive psychology service-learning course. However, to date, no study …

Exploring Staphylococcus Aureus Host-Pathogen Interaction Using Proteomic Technologies, Emilee Marlene Elizabeth Mustor

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus deploys an arsenal of virulence effectors to remodel the host environment during infection, while simultaneously the host mounts an immune response to contain and eliminate this bacterium. This dynamic interplay defines the host-pathogen interface and shapes infection outcome. In this context, the secreted proteases represent a major arm of S. aureus pathogenesis, promoting bacterial persistence in the host by dismantling immune defenses, cleaving host signaling molecules and reshaping the host proteome. However, traditional substrate-by-substrate characterization cannot reveal the full scope of proteolytic activity occurring during infection. To address this, we developed Terminal Amine-Guanidination of Substrates-Charge Reversal (TAGS-CR), a …

Multi-Modal Survival Prediction On Breast Cancer Mammograms And Enabling Multi-Institutional Collaborations With Federated Learning, Nikolas Koutsoubis

USF Tampa Graduate Theses and Dissertations

Cancer remains one of the leading causes of mortality worldwide, and machine learning is well positioned to leverage the large volumes of routinely collected clinical and imaging data to improve patient outcomes. Realizing this potential at scale requires three capabilities that current practice does not yet deliver in combination: models that integrate the multiple data modalities clinicians use, training procedures that respect institutional data-sharing constraints, and tooling that enables sharing of de-identified data when federated approaches are not sufficient. This dissertation develops methods and infrastructure addressing each of these capabilities in the context of breast cancer and oncology more broadly. …

Testimonios From Latine Educational Leaders Supporting Youth Development Programs, Luis E. Feliciano

USF Tampa Graduate Theses and Dissertations

The United States has been experiencing a demographic change for the last twenty years representing a rise in both Latiné students and families. Despite this demographic change, Latiné folk remain widely underrepresented in school leadership, and furthermore underrepresented in specific Youth Development Programs (YDPs), with the relationship between educational leaders and YDPs being widely unexplored in academia. This study sought to bridge the gap between YDPs and educational leadership, specifically for Latiné folk by asking how Latiné educational leaders used their cultural heritage to encourage YDP development. To accomplish this a testimonio methodology was implemented via a two staged interview …

Evaluating The Role Of Individual Differences: Impact On Fruit And Vegetable Intake Outcomes In Nourish-T+ Caregivers Of Pediatric Cancer Survivors, Sandra Soca Lozano

USF Tampa Graduate Theses and Dissertations

Pediatric cancer survivors are at increased risk for obesity and related long-term health complications, highlighting the need for interventions promoting healthy lifestyle behaviors during survivorship. The present study examined caregivers’ fruit and vegetable intake in the full NOURISH-T+ intervention and evaluated whether their dietary outcomes changed from baseline to the 3-month post-assessment and differed by selected caregiver sociodemographic characteristics. The study also examined whether these characteristics accounted for variance in 3-month fruit and vegetable outcomes. Participants included 68 caregivers in the full NOURISH-T+ condition who completed both baseline and 3-month post-assessments. Fruit and vegetable intake was assessed using the Healthy …

Building Trustworthy Information Systems: A Unified Framework For Comparative Risk Detection, Parisa Momeni

USF Tampa Graduate Theses and Dissertations

Risk detection in large scale information systems increasingly depends on heterogeneous data generatedby both centralized and distributed ecosystems. While centralized systems provide curated and validated reports, distributed environments produce large-scale and real-time observational evidence. Existing computational approaches analyze these ecosystems in isolation, limiting systematic comparison of risk repre-sentations across heterogeneous sources.

This dissertation presents a unified computational framework for comparative risk detection across centralized and distributed information systems. The framework provides a domain independent methodology for transforming heterogeneous risk reporting data into comparable multidimensional representations. To enable interpretable comparison of heterogeneous risk distributions, this work introduces the Geometric Overlap Score …

Language Models For Oncology Clinical Text: How Model Architecture And Data Strategies Shape Tumor Phenotype Extraction And Disease Progression Detection, Thanh Duong

USF Tampa Graduate Theses and Dissertations

The rapid growth of electronic health records (EHRs) has created new opportunities to apply machine learning to clinical data.However, a large portion of important clinical information is still stored in unstructured text, such as pathology reports, radiology reports, and longitudinal clinical notes.These documents contain key details about tumor characteristics, diagnoses, treatments, and patient outcomes.Extracting structured and useful information from this text is challenging due to complex medical language, varied document formats, and the need to combine information across multiple reports over time.This dissertation studies how language models can be designed and adapted to better extract and use oncology-specific information from …

House Sparrow Invasion Success Across Ecological, Immunological, And Molecular Scales, Kailey Marie Mccain

USF Tampa Graduate Theses and Dissertations

Biological invasions offer natural experiments for studying how organisms cope with ecological novelty. Although invasion success is often attributed to enemy release, introduced organisms do not enter enemy-free environments. Instead, they enter heterogeneous landscapes in which some familiar enemies are absent, others remain, and entirely novel enemies may be encountered. The question, then, is how invaders (hereafter non-natives) are poised to respond to these threats? This dissertation examines whether successful invasions in house sparrows (Passer domesticus) is associated with shifts in pathogen exposure, immune dynamics in blood, gut physiology, and gene co-expression network structure.

Chapter 1 is at the population/ecological …

Mapping Seafloor Habitats And Characterizing Demersal Reef Fish Communities On The West Florida Shelf Across Multiple Spatial Scales Using Multibeam Sonar And Underwater Video, Alexander R. Ilich

USF Tampa Graduate Theses and Dissertations

In this dissertation, I examine the role of spatial scale in deriving seafloor terrain attributes, modeling benthic habitat, and analyzing ecological patterns of the demersal reef fish communities on the West Florida Shelf (WFS), with implications for improving predictive habitat mapping and understanding fish-environment relationships. Spatial scale plays a central role in benthic habitat mapping and ecological inference, influencing how terrain attributes are derived, how habitats are modeled, and how ecological responses are manifested. This work integrates multiscale terrain analysis, acoustic remote sensing, and community ecology to evaluate how spatial scale influences both physical habitat representation and ecological analysis.

Chapter …

Association Of Social Isolation, Loneliness, Social Strain And Support With Cancer Onset, And Frailty And All-Cause Mortality In People With Cancer, Joanne Elayoubi

USF Tampa Graduate Theses and Dissertations

Cancer is the second leading cause of death both in the United States and globally and for individuals over sixty-five. In 2026, approximately 2,114,850 new cancer cases are projected to emerge according to the National Center for Health Statistics (Siegel et al., 2026). Cancer incidence is increasing, making older adults sixty-five and older at risk of frailty and mortality following cancer. Although social isolation, loneliness, lack of social support, and social strain have been found to be risk factors for poor health and mortality, it is not clear the extent to which social relationships are associated with incident cancer, and …

Examining The Referral Pathway For Black Families Evaluated Through The Early Steps Program, Zelda Fleming

USF Tampa Graduate Theses and Dissertations

Research indicates that for children with or at risk for developmental delays, early intervention services are associated with improvements in academic and cognitive skills by the time they reach kindergarten (Atkins et al., 2020; Litt et al., 2017). Untreated developmental delays are linked to early school failure and social and emotional problems (Sher et al., 2020). Black children are 5 times less likely to receive early intervention services when compared to their White peers (Barnard-Brak et al., 2021; Feinberg et al.,2011). Thus, they often do not have the opportunity to receive early intervention services. The literature in this area suggests …

Designing For Fitness And Resilience In Human Artificial Intelligence Systems, Thomas R. Gill

USF Tampa Graduate Theses and Dissertations

Over the last decade, new artificial intelligence models, frameworks, and applications have emerged that have radically changed the socio-technical landscape. These new technologies represent both great opportunities and great potential risks. Due to the recency of their emergence, there is little known about the longevity and long-term repercussions of artifacts powered by new artificial intelligence technology. In this dissertation, I evaluate A.I. and A.I.-based artifacts through the lens of the fitness-utility model, a tool developed by Gill and Hevner (2013) for the evaluation of an artifact's ability to survive and reproduce over long time horizons. In so doing, I also …

Photoluminescent Studies Of Metal-Organic Frameworks For Small Molecule Sensing, Julia T. Dematteo

USF Tampa Graduate Theses and Dissertations

Metal–organic frameworks (MOFs) are highly tunable nanoporous materials with broad applications in gas separation, drug delivery, light harvesting, and chemical sensing. The incorporation of photoactive components into MOFs enables the development of optical sensors capable of detecting a wide range of analytes. Porphyrins and metalloporphyrins are particularly attractive for such applications due to their high molar absorptivity, strong emission quantum yields, and versatile interactions with chemical species.

In this work, two porphyrinic MOFs, PCN-222 and MOF1(Cd), are investigated. Both frameworks utilize tetrakis(4-carboxyphenyl)porphyrin (TCPP) as the organic linker, imparting optical functionality to the materials. Integration of TCPP into the framework induces …

Development And Initial Validation Of The Identifying Behavioral And Emotional Growth Indicators And Needs—Parent Rating (I-Begin-P), Samin Khallaghi

USF Tampa Graduate Theses and Dissertations

Multi-informant approaches provide a more comprehensive understanding of behavior patterns and are considered the gold standard for behavioral assessment. However, few screening tools for young children in school settings incorporate parental perspectives, and those that do remain under-researched with respect to their validity and reliability. To address these gaps, the Identifying Behavioral and Emotional Growth Indicators and Needs-Parent Form (I-BEGIN-P) was developed as an extension of the I-BEGIN-T (Teacher) scale for children aged 3 to 5 years. The purpose of this study was to develop and initially validate I-BEGIN-P. First, the content validity of I-BEGIN-P was assessed using expert panel …

Reproductive Trends Of Male Atlantic Spotted Dolphins (Stenella Frontalis) Following Two Hurricanes In The Bahamas, Tara Southerland Pedersen

USF Tampa Graduate Theses and Dissertations

Marine mammals are impacted by hurricanes that may influence reproductive success and genetic diversity. Understanding how populations respond to demographic change is increasingly important as extreme weather events become more frequent. A population of Atlantic spotted dolphins (Stenella frontalis) on Little Bahama Bank in the Bahamas has been observed continuously since 1985. In 2004, two hurricanes struck the region within one month, resulting in the loss of approximately 36% of the population. Population declines can alter the operational sex ratio (OSR), social structure, and encounter rates among potential mates, potentially influencing patterns of reproductive success. Previous molecular studies showed that …

Photophysical Behavior Of Ruthenium-Based Complexes: A Study Of Ligand Variation And Encapsulation In Metal-Organic Frameworks, Delaney S. Sellers

USF Tampa Graduate Theses and Dissertations

Ruthenium (II) polypyridyl complexes have emerged as a prominent class of compounds in inorganic photochemistry due to their structural robustness, reversible redox chemistry, and tunable excited-state dynamics. Central to the investigation of these systems is the ability to manipulate the triplet metal-to-ligand charge transfer state (3MLCT). In the context of photoactivated chemotherapy (PACT), the strategic deactivation of this MLCT state to populate a dissociative triplet ligand field state (3LF) can enable the controlled, light-triggered release of caged cytotoxic agents. This work focuses on understanding the caging mechanism of various ligands to optimize the eventual delivery of cytotoxic ligands. In this …

Development Of A Coupled Hydrological-Nitrogen Modeling Framework For Urban Stormwater Pond Analysis, Tione Grant

USF Tampa Graduate Theses and Dissertations

Urbanization impacts the hydrological and biogeochemical processes that regulate water quality in downstream ecosystems, leading to increased stormwater volume and nitrogen loads in receiving aquatic environments. Although stormwater ponds are designed as simple hydraulic storage basins, they function more like managed ecosystems within urban drainage systems, offering both hydraulic attenuation and passive nutrient treatment. However, nitrogen removal efficiency varies significantly and depends on interactions between hydrological and biogeochemical factors. This thesis details the development, calibration, validation, and sensitivity analysis of a coupled hydrological-nitrogen model framework for Aaran’s Pond, an urban stormwater pond in Hillsborough County, Florida.

A process-based water budget …

Under Pressure: Effects Of The Hot Docs Behavioral Parent Training Program On Caregiver Blood Pressure, Bria N. Ferera

USF Tampa Graduate Theses and Dissertations

Chronic stress is heightened for caregivers of children with heightened disruptive behaviors. With chronic stress supported as a major factor leading to maladaptive parenting and caregiving practices, exploring new ways to collect data on parenting stress remains a critical research priority (van der Lubbe et al., 2024). Behavioral Parent Training (BPT) programs are linked to improved parent-child relationships and reduced problem behavior (Armstrong, 2006; Agazzi, 2021; Patrusky, 2025). However, few studies have used physiological biomarkers in conjunction with self-reported measures to explore connections in this area. Similarly, other physiological considerations are rarely considered upon evaluation of the impacts of BPT …

Trophic Structure Of Reef-Fish Assemblages In The Eastern Gulf Of Mexico Varies By Depth And Reef Type, Jessica L. Van Vaerenbergh

USF Tampa Graduate Theses and Dissertations

Globally, marine ecosystems are increasingly threatened by anthropogenic stressors, leading to reef degradation, changes in reef-fish assemblages, and altered trophic structure. In this study, I investigated reef-fish underwater visual survey data collected over a decade (2013-2023) from paired natural and artificial reefs in the eastern Gulf of Mexico. I applied the novel Normalized Reef Status Index (NRSI) to evaluate how biomass was distributed across the three trophic categories (i.e., Lower Trophic Position (LTP; 2.0-2.9), Central Trophic Position (CTP; 3.0-3.9), and Upper Trophic Position (UTP; ≥4.0). This index is a metric to assess the ecological status of reef systems by investigating …

In Search Of Protective Factors Against Gun-Carrying For Black Male Youth: A Dissertation- Sectional And Longitudinal Analysis Of Secondary Data, Kevin Edward Antwi

USF Tampa Graduate Theses and Dissertations

This study examines the relationship between risk and protective factors and gun carrying among Black male youth using both individual-level and population-averaged effects. Grounded in the risk and protective factors framework and the ecological systems model, the study explores how protective factors at the individual, peer, school, and community levels influence gun carrying at a single point in time and over time. This research aligns with the Grand Challenges of Social Work by preventing gun violence and informing prevention efforts. Using the baseline and 11 waves of data from the Pathways to Desistance Study, the analysis identifies factors that reduce …

From Cardiovascular Safety Assessment To Peptide Hemolytic Activity Prediction: Integrative Computational Analyses For Drug Evaluation And Discovery, Xinran Zhu

USF Tampa Graduate Theses and Dissertations

This thesis applied integrative computational approaches to two major areas of drug evaluation and discovery: cardiovascular safety assessment and peptide hemolytic activity prediction. First, pharmacovigilance data from the FDA Adverse Event Reporting System (FAERS), electronic health records (EHRs) from TriNetX, and transcriptomic analyses were combined to evaluate anesthetic-associated cardiac rhythm adverse drug events. Among propofol, ketamine, dexmedetomidine, and midazolam, dexmedetomidine showed the strongest association with bradycardia across both FAERS and propensity score-matched EHR analyses. Stratified analyses further suggested substantially higher bradycardia prevalence among older patients and a modestly increased risk among male patients. Drug-drug interaction analyses identified risperidone and albuterol …

“He’S Got The Whole World In His Hands”: Seed Sovereignty And Agricultural Decision Making In Southeastern Kenya, Caroline Stahley

USF Tampa Graduate Theses and Dissertations

Increasing corporate control of the global seed supply and restrictions to local exchange threaten the autonomy of smallholder farmers and Indigenous communities. This project contributes to agricultural sovereignty movements by examining the decisions farmers make to address climate uncertainty, market volatility, and development agendas. Specifically, how is seed saving practiced and understood in relation to sovereignty? It reimagines what global food systems might look like if investment were directed to producers rather than intermediaries, arguing that cultivators lack support, not knowledge or capacity. Across these domains, seeds act as a motif to describe the concerns, innovations, and visions of farmers.

House Sparrows Reveal Hidden Reservoirs Of Antimicrobial Resistance, Nathan Dowling

USF Tampa Graduate Theses and Dissertations

Antimicrobial resistance (AMR) is a growing global health threat, yet surveillance for antimicrobial resistance genes (ARGs) remains concentrated in clinical and livestock systems, leaving environmental and wildlife reservoirs comparatively understudied. The objective of this study was to evaluate whether one synanthropic bird species integrates landscape-level antimicrobial resistance pressures across socioeconomic gradients and to identify the predictors most strongly associated with cross-national variation in ARG prevalence. We examined ARG occurrence in the globally distributed house sparrow (Passer domesticus), sampling 205 individuals across eight countries spanning large gradients in economic development, antimicrobial use, and governance structure. Using targeted molecular screening of gastrointestinal …

Amending Planting Media With Typha-Derived Biochar For Sustainable Horticulture, Elissa Touma

USF Tampa Graduate Theses and Dissertations

Constructed wetlands are widely used to improve water quality by removing excess nutrients from wastewater and stormwater. Nutrients are stored within wetland vegetation, particularly in fast-growing plants such as Typha. Harvesting vegetation provides an opportunity to permanently remove these nutrients while generating biomass suitable for beneficial reuse. Converting harvested biomass into value-added products such as compost and biochar can support nutrient recovery and sustainable biomass management. In addition, increasing concerns regarding the environmental impacts of peat extraction have created interest in renewable alternatives for horticultural growing media. Therefore, the objective of this study was to evaluate the potential of harvested …