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Torsion-Induced Pressure Distribution Changes In Human Intervertebral Discs: An In Vitro Study, Brenda Kay Yantzer

USF Tampa Graduate Theses and Dissertations

Introduction. To test the effects of torsion torques on intradiscal pressure and disc height in human lumbar specimens.

Methods. Six human lumbar cadaveric functional spine units (FSU) were loaded in the neutral position with 600 N compression. Nucleus pressure measurements were obtained at 0 Nm, 0.5 Nm, 1.0 Nm and 2 Nm torsion torque. Posterior elements were removed and pressure measurements were repeated at the same torsion torques for the disc body unit (DBU). The pressure in the nucleus was measured by pulling a pressure probe through the disc along a straight path in the midsagittal plane.

Results …

Sialic Acid Modulation Of Cardiac Voltage-Gated Sodium Channel Gating Throughout The Developing Myocardium, Patrick J. Stocker

USF Tampa Graduate Theses and Dissertations

The proper orchestration of voltage-gated ion channel gating is vital to maintaining normal heart rhythms throughout an animal's lifespan. Voltage-gated sodium channels, Na_v, are responsible for the initiation of the cardiac action potential, which leads to cardiac systole. Comparison of neonatal ventricular and atrial myocyte Na_v gating with adult indicated that the neonatal ventricular Na_v gated following a ~10 mV greater depolarization than did atrial or adult ventricular Na_v. In this study I questioned whether development- and/or chamber-dependent changes in Na_v-associated functional sialic acids could account for these differences. When desialylated with …

An Exploration Of The Lumbar Loads And Affective Responses To Lumbar Pain On Lower Limb Amputees Who Use A Prosthesis, Tracy Ann Perrotti

USF Tampa Graduate Theses and Dissertations

80% of the American population experiences back pain and it is the most common cause of limited activity in people of age 45 and under. Determining the reasons for back pain and developing new ways to treat it have been extensively researched over the past decade. However, very little research has been done on low back pain of amputees.

There are four million existing amputees living in America and 250,000 people become new amputees each year. 70% of this group is lower limb amputees and a large number use a prosthesis of some kind to aid in the functions of …

Stress And Cognitive Performance In Older Adults, Christopher B. Rosnick

USF Tampa Graduate Theses and Dissertations

The current dissertation sought to examine stress in three different, but overlapping, ways. The first study examined how self-reported negative life events, in the aggregate and individually, are associated with cognitive performance. The results suggested that there was no significant relationship between the aggregate measures of self-reported negative life events and cognitive performance. On the other hand, several individual negative life events were associated with cognitive functioning. The findings support previous research indicating that using estimates of individual stressors rather than aggregate measures of stressors increases the predictive validity of stress measurement.

The second study assessed the cross-sectional and longitudinal …

Zooplankton Of The West Florida Shelf: Relationships With Karenia Brevis Blooms, Kristen M. Lester

USF Tampa Graduate Theses and Dissertations

Blooms of the toxic dinoflagellate Karenia brevis are common on the West Florida Shelf (WFS), yet little is known of the relationships between zooplankton and K. brevis. A comprehensive analysis was undertaken to examine 1) perturbations in zooplankton community composition within K. brevis blooms 2) the contribution of zooplankton ammonium and phosphate excretion to K. brevis bloom nutrient requirements, and 3) the role of zooplankton grazing in K. brevis bloom termination. Prior to undertaking the first portion of the study, an examination of the perturbations in the normal zooplankton assemblage within K. brevis blooms, it was first necessary to …

Expanding The Science Of Successful Aging: Older Adults Living In Continuing Care Retirement Communities (Ccrcs), Kathryn H. Petrossi

USF Tampa Graduate Theses and Dissertations

Rowe and Kahns theory of successful aging identifies three main components of aging successfully: reducing the risk of disease and disability, maintaining high cognitive and physical function, and engagement with life. While there is compelling evidence that suggests the legitimacy of this concept in the 50-75 year old community dwelling population, three areas of expansion are necessary: 1.) programmatic research; 2.) extending the existing research samples to include older samples and those living in continuing care retirement communities (CCRCs); and 3.) the integration of data collection and analysis to move beyond investigation of just one successful aging outcome to include …

Establishing A Farmers Market For A Low-Income Latino Community, Ann L. Bretnall

USF Tampa Graduate Theses and Dissertations

For the past decade, Florida’s Latino population has significantly increased and is now the third largest in the United States. The same trend has also occurred in Hillsborough County. Social and economic disparities are significant as Latinos earn less than non-Hispanic whites and many live in poverty. A major concern of this population is the lack of access to inexpensive fresh fruits and vegetables. Findings from prior research show that the diet of immigrants often change quickly upon their arrival to the United States, with an increased emphasis on fast food and soft drinks and a reduction in the consumption …

“They Say That This Clinic Is For Migrants”: Cultural Sensitivity In A Rural Health Center, Nadine I. Ohlinger

USF Tampa Graduate Theses and Dissertations

The growing number of minority populations living in the United States makes it mandatory that all health care organizations seek to be culturally sensitive. There is no consensus on the definition of the term cultural sensitivity. The goal of this thesis is to define what cultural sensitivity means in a rural health center, from the perspective of the staff as well as the Hispanic patient. Anthropological methods, such as participant observation, semi-structured interviews, and archival data analysis, show that the qualities that Hispanic patients value in a clinic are 1) attention, 2) availability of Spanish language, 3) financial assistance, 4) …

Association Between Area Socioeconomic Status And Hospital Admissions For Childhood And Adult Asthma, Tomas Tamulis

USF Tampa Graduate Theses and Dissertations

Despite an improved understanding of the disease, the prevalence of asthma and asthma-related morbidity continue to rise, particularly among minority and inner-city populations. Despite the growing epidemic of asthma, the surveillance of disease at the state or even local levels is very limited. Such information is very important to identify high-risk population groups and to design more effective community-based preventive interventions or risk management programs that may modify these trends.

The study provided important information about spatial differences by the geographical area of residence and changes in asthma hospital admissions over time in the selected area. Environmental exposure to ambient …

More Than Man’S Best Friend: A Look At Attachment Between Humans And Their Canine Companions, Samantha E. Kennedy

USF Tampa Graduate Theses and Dissertations

According to the American Veterinary Medical Association, there are currently more than 60 million pet dogs in the United States. This is an increase of nearly eighteen percent since 1991, coinciding with a growing area of research on human’s relationships with companion animals and companion animals’ place in society.

For years dogs have been thought of as “man’s best friend” because of their loyalty and faithfulness. The increasing popularity of activities such as canine daycare and puppy school suggests that dogs have become more than a best friend to some and even an integral part of the American family unit. …

Comparison Of Hatching Failure In A Wildland And Suburban Population Of The Florida Scrub-Jay (Aphelocoma Coerulescens), Sonya Christine Leclair

USF Tampa Graduate Theses and Dissertations

Egg hatchability has been correlated with many factors, including clutch size, presence of helpers, timing of breeding and predation risk. Hatching failure is higher in a suburban population of Florida Scrub-Jays than in a wildland population, but the reasons for this pattern are unclear. An analysis of long-term demographic data on scrub-jays in both habitats revealed the factors that best explained variation in hatching failure, and an experiment tested whether two potential site factors, ambient temperature and predation risk, could increase hatching failure in the suburbs.

Although a global model was best supported by the data for occurrence of partial …

Lysogeny: Practical Applications And New Discoveries., Lauren Mcdaniel

USF Tampa Graduate Theses and Dissertations

Part 1:

Prophage induction has been demonstrated to be a sensitive indicator for a wide variety of toxic and mutagenic compounds and, as a consequence, has been utilized for biologically based carcinogen screenings. Fourteen marine bacterial isolates were screened for development into the Marine Prophage Induction Assay (MPIA), for marine samples. The selected isolate (P99-4S3) was identified by 16S rDNA sequencing as Pseudomonas aeruginosa. This isolate demonstrated a log-linear response to increasing dose of mutagens, and sensitivity to known environmental contaminants. Field-testing of the assay over two years demonstrated the MPIA would be a useful screening tool for environmental …

Generation Of Carbon Dioxide And Mobilization Of Antimony Trioxide By Fungal Decomposition Of Building Materials, John D. Krause

USF Tampa Graduate Theses and Dissertations

Fungal contamination of buildings poses numerous challenges to researchers, building owners and occupants. Public health agencies promote prevention and remediation of mold and water damage, but lack sensitive methods to detect hidden mold growth and a complete understanding of the biological mechanisms that make occupying moldy buildings a hazard. The wide spread use of the fire retardant antimony trioxide (Sb₂O₃) on building materials and furnishings makes it inevitable that mold growth on treated materials will occur in some buildings with water damage. Several authors have speculation that microbial growth on materials treated with antimony trioxide could …

Effect Of Chromium Vi On The Production And Behavior Of Lytechinus Variegatus (Echinodermata: Echiniodea), Jennifer Rhora

USF Tampa Graduate Theses and Dissertations

Small amounts of chromium (VI) are carcinogenic in mammals. Concentrations of Cr in marine algae and seagrasses range from 0.06-7.17 /g DW and 0.1-30.6 g/g DW respectively. To test for an effect of these concentrations, production (change in organic material), righting response, feeding rates, absorption efficiency and fecal production were measured in Lytechinus variegatus from Sarasota fed prepared diets containing 0, 4.1, and 32g Cr/ g DW and individuals from Ft. DeSoto fed diets containing 0, 41 and 82g Cr/ g DW. The urchins were fed for 4-5 weeks, with weekly measurements of their feeding rates, absorption efficiency and fecal …

Bottom-Up And Top-Down Effects On Insects Herbivores Along A Natural Salinity Gradient In A Florida Salt Marsh, Maria Teresa Albarracin

USF Tampa Graduate Theses and Dissertations

I compared the strength of bottom- up and top-down effects on insect herbivores along a natural salinity gradient in salt marsh communities in West - Central, Florida. I used a 2x2 factorial design with plots divided into four different treatments: 1) fertilizer applied to increase plant quality 2) sticky traps added to remove natural enemies (parasitoids) 3) fertilizer applied and sticky traps added and 4) control plots. These plots were placed on 7 different sites containing the salt marsh plant Borrichia frutescens along a natural stress salinity gradient. In each plot I determined the abundance of the sap sucker Pissonotus …

School-Based Mental Health Services Delivered By School Psychologists, Emily Luis

USF Tampa Graduate Theses and Dissertations

This study involved analyses of an existing database conducted for the purposes of identifying and understanding factors that are associated with the delivery of school-based mental health services by school psychologists. The study examined the average number of hours per week in which school psychologists engage in mental health services and the types of mental health services in which they engage. Factors such as training, desire to deliver mental health services, time available and support for mental health services were investigated. The database that served as the basis for these analyses had been created by mailing a survey to 1000 …

Do Expectancies Influence Outcomes For Tailored Smoking Cessation Messages? A Placebo Tailoring Experiment, Monica S. Webb

USF Tampa Graduate Theses and Dissertations

This study was an effort to replicate and extend findings from our previous research, which suggested that the efficacy of tailored messages is influenced by high levels of content personalization within the message and by individuals trait expectancies about tailored interventions. We tested whether tailoring-related expectancies regarding smoking-cessation booklets could be altered via a brief expectancy-priming intervention, and whether this would enhance the impact of the cessation materials. A 2x2 factorial design crossed personalization level and expectancy priming on evaluation of the intervention content, readiness to quit smoking, cessation self-efficacy, cognitive processing, and progress towards quitting. Smokers (N = 205) …

An Examination Of The Diet And Movement Patterns Of The Atlantic Cownose Ray Rhinoptera Bonasus Within A Southwest Florida Estuary, Angela Barker Collins

USF Tampa Graduate Theses and Dissertations

Cownose rays are benthic, suction feeders whose foraging activities have been implicated in severe damage to commercial shellfish industries and seagrass habitat. With jaws highly modified for durophagy, it has been assumed that they are crushing specialists, feeding primarily upon hard molluscan prey. In addition, R. bonasus are believed to be highly migratory, transient residents of coastal inshore waters. However, minimal quantitative data exist regarding R. bonasus feeding or movement patterns in the Gulf of Mexico. Stomach contents from 50 cownose rays caught within the Charlotte Harbor estuary between July 2003 and July 2004 were analyzed using the index of …

Dissecting Out The Contribution Of Cognitive, Social, And Physical Activities To Environmental Enrichment’S Ability To Protect Alzheimer’S Mice Against Cognitive Impairment, Jennifer R. Cracchiolo

USF Tampa Graduate Theses and Dissertations

Retrospective studies suggest that lifestyle activities may provide protection against Alzheimer s Disease (AD). However, such studies can be inaccurate and prospective longitudinal studies investigating lifestyle protection against AD are both impractical and impossible to control for. Transgenic (Tg+) AD mice offer a model in a well controlled environment for testing the potential for environmental factors to impact AD development. In an initial study, Tg+ and non-transgenic (Tg-) mice were housed in either environmentally enriched (EE) or standard housing (SH) from 2-6 months of age, with a behavioral battery given during the last 5 weeks of housing. In the Morris …

Coral Reef Assessment: An Index Utilizing Sediment Constituents, Camille A. Daniels

USF Tampa Graduate Theses and Dissertations

Resource managers need inexpensive bioindicators to evaluate the health of coral reef ecosystems and to inform decisions on when and where to utilize more expensive assessment techniques. Following USEPA Guidelines for Evaluating Ecological Indicators, I developed the SEDCON Index (SI), a rapid-assessment protocol which utilizes reef sediment composition to assess the integrity of coral-reef communities. Key advantages of this index are that it entails non-destructive sampling and is applicable to reefs worldwide. The underlying assumption of the index is that community structure is reflected by proportions of recognizable remnants of calcareous shells and skeletal remains of mixotrophic (zooxanthellate corals and …

Extrapulmonary Tuberculosis In Hiv-Positive And Hiv-Negative Children In Haiti: A Hospital-Based Investigation, Marie F. Denis

USF Tampa Graduate Theses and Dissertations

Introduction: Globally, one in four persons infected with the human immunodeficiency virus (HIV) who are living with the acquired immunodeficiency syndrome (AIDS) will die of tuberculosis (TB). The estimated number of HIV-infected children who die of tuberculosis, especially extrapulmonary TB (EPTB), in Haiti, is only loosely based on facts or investigation. This study proposes to describe demographics of children with EPTB in a pediatric TB hospital in Haiti. The objectives are two-fold. The first objective is to describe the population of children discharged from Grace Children’s Hospital with a confirmed diagnosis of tuberculosis overall, and broken down by whether or …

The Influence Of Stakeholder Groups On The Decision Making Process Regarding The Dead Zone Associated With The Mississippi River Discharge, Lisa M. Fairchild

USF Tampa Graduate Theses and Dissertations

The Action Plan for Reducing, Mitigating, and Controlling Hypoxia in the Northern Gulf of Mexico represents the first national attempt to address this environmental issue. Hypoxia is the condition of low concentrations of dissolved oxygen in a body of water. This condition leads to a so-called “dead zone” and potentially threatens industries dependent on the living marine resources of this area. The potential impact of any policy or plan designed to combat hypoxia in the Northern Gulf of Mexico could have significant impacts on stakeholder groups, specifically the fishing industry in the Gulf of Mexico, and the agriculture and fertilizer …

Aortic Valve Analysis And Area Prediction Using Bayesian Modeling, Miheer S. Ghotikar

USF Tampa Graduate Theses and Dissertations

Aortic valve stenosis affects approximately 5 out of every 10,000 people in the United States. [3] This disorder causes decrease in the aortic valve opening area increasing resistance to blood flow. Detection of early stages of valve malfunction is an important area of research to enable new treatments and develop strategies in order to delay degenerative progression. Analysis of relationship between valve properties and hemodynamic factors is critical to develop and validate these strategies.

Porcine aortic valves are anatomically analogous to human aortic valves. Fixation agents modify the valves in such a manner to mimic increased leaflet stiffness due to …

Full-Page Versus Partial-Page Screen Designs In Web-Based Training: Their Effects On Learner Satisfaction And Performance, Phillip Eulon Grace

USF Tampa Graduate Theses and Dissertations

This is a report on research regarding the screen layout of Web-based training (WBT) programs, conducted with an eye toward providing evidence-based guidance for the design and development of WBT interfaces. Specifically, the study investigated the relative instructional benefits of two general types of WBT screen design, full-page and partial-page, in terms of both learner performance and learner satisfaction. The main hypotheses of the study were that the full-page design option would yield significantly better outcomes in both categories of interest.

The study employed a mixed-method design, generating both quantitative and qualitative data. The main phase of the study was …

Prehistoric Human Subsistence Patterns In Northern Patagonia, Argentina: Isotopic Evidence For Reconstructing Diet, Scott Grammer

USF Tampa Graduate Theses and Dissertations

This study investigates the isotopic signatures of human skeletal remains that were recovered from several sites along the coast and inland in the north-central Patagonian region of Argentina. Human skeletal remains, dating from 2500 BP through the early historic period, are examined to determine the relative significance of terrestrial and aquatic food resources and subsequently, the extent to which coastal food resources were exploited by indigenous Argentinians. Carbon and nitrogen isotopes contained within human bone collagen and apatite are measured quantitatively to determine the relative significance of marine and terrestrial foods. This study, one of the first isotopic studies of …

The Effects Of A Comprehensive Post-Treatment Recovery Program For Breast Cancer Survivors, Keri Ann Hockett

USF Tampa Graduate Theses and Dissertations

Background: Breast cancer and its treatment often result in side effects that persist long after treatment has ended. The increased survival rate for breast cancer has allowed for the study of the physical and psychosocial symptoms that persist into the post-treatment period. Although research has tested various interventions and demonstrated improvement in some symptoms, no standard of care exists for management of symptoms in the post- treatment period as part of the continuum of care. Objective: The aim of this research was to examine the effects of a comprehensive recovery program of education, exercise, and support for breast cancer survivors …

Influence Of Gender On Heart Rate And Core Temperature At Critical Wbgt For Five Clothing Ensembles At Three Levels Of Metabolic Rate, Maeen Zakaria Islam

USF Tampa Graduate Theses and Dissertations

Three main factors that influence heat stress are clothing, work demands and environmental conditions. Gender may also influence the amount of heat stress an individual can tolerate. The purpose of this study was to examine the role of gender in heat stress limits (critical WBGT) and heat strain (heart rate and core temperature). The null hypothesis was that there was no gender difference among critical WBGT, heart rate and core temperature.

Fifteen subjects (11 men and 4 women) wore five different clothing ensembles (cotton work clothes, cotton coveralls, particle barrier Tyvek, water-barrier/vapor permeable NexGen LS417, and vapor barrier Tychem QC …

The Impact Of Privatization Of Primary Care Programs In Large County Health Departments In Florida, Arlesia Lynn Brock

USF Tampa Graduate Theses and Dissertations

Since the mid-1970s, top managers, politicians, and officials in both public and private institutions have promoted contracting-out services (privatization) as a means of increasing efficiency, flexibility, and quality. The privatization trend has occurred in many public sector organizations particularly in city services and prisons. Public health services are not immune to this trend. Today many county health departments have contracted the provision of public health services like womens health, primary care, and laboratory services. However, very few studies have analyzed the impact of these privatizations on cost, access, and health outcomes. Proponents favoring the private provision of these services argue …

Social Construction Of Cervical Cancer Screening Among Women In Panama City, Panama, Arlene Calvo

USF Tampa Graduate Theses and Dissertations

To learn how to address health issues specific to Hispanic cultures in an effort to address health disparities, learning from cultural aspects that affect health from the countries of origin would be most useful. Community programs built on rigorous and systematic research prove to be more powerful than ad-hoc programs. Qualitative research techniques offer powerful alternatives for public health professionals to develop adequate and directed programs at the community level, especially among underserved communities and those represented by oral/spoken traditions. The study was conducted among 132 working class single and married Mestizo women between the ages of 20-40 living in …

Globalization, Ecotourism, And Development In The Monte Verde Zone, Costa Rica, Edgar Allan Amador

USF Tampa Graduate Theses and Dissertations

Ecotourism has been promoted globally as a model for sustainable development because it simultaneously benefits the environment and the residents of the given destination. However, many conservationists have questioned the long term sustainability of ecotourism as it is difficult to mitigate the impact of even low levels of tourism on a particular ecosystem. Further, social scientists including anthropologists have similarly questioned whether most residents of a particular destination actually benefit significantly from the alternative tourism economy.

The Globalization Research Center in cooperation with the Monteverde Institue in the Monte Verde Zone, Costa Rica, is undertaking a longitudinal study -- dubbed …