Digital Commons Network^™

Coarse-Grained Modeling Studies Of Entangled Semiflexible Polymers: Melts, Glasses, And Granular Media, Joseph Fox D. Dietz

USF Tampa Graduate Theses and Dissertations

This dissertation is devoted to the computational study of model semiflexible polymers using a coarse-grained bead-spring approach and molecular dynamics (MD) simulations. Much of the work presented here is motivated by the need to derive predictive models for describing polymeric materials’ macroscopic mechanical response from the underlying local structure or local chain parameters. This dissertation describes polymeric materials in the context of granular media, melts, and glasses with a focus on relating characteristics such as chain stiffness and local structure to systems’ solidification, rheology, and fracture mechanisms.

Chain stiffness κ and structural properties like chains’ characteristic bond angle θ0 are …

African American Males' Perception Of Factors That Contribute To Success In Higher Education, Gary D. Oliver

USF Tampa Graduate Theses and Dissertations

Over the past decades, many studies have concluded that African American students' college completion rate and success lag far behind other students attending college in the United States (The JBHE Foundation, Inc., 2006). More specifically, these studies have confirmed that African American male students' success rates remain disproportionally low compared to other ethnic male groups. Unfortunately, few notable studies identifying African American males achieving higher education or completing their academic pursuits have been presented as part of the Black male student narrative.

This study aimed to understand better the resources and experiences that positively affect African American males who completed …

Using Online Reviews To Identify How Hotels Can Satisfy Travelers With Pets While Making Money, Sonia Weinhaus

USF Tampa Graduate Theses and Dissertations

A Qualitative Content Analysis of user generated content in the form of online reviews reveals an opportunity for hotels to better serve Travelers With Pets and collect partitioned fees. Examining more than 3,000 reviews of 92 hotels from five geographical regions of the United States identifies how to close the gap between what customers want and what hotels offer. This study derives the Minimum and Maximum Typologies of pet policy structures from the coding of online reviews. Travelers With Pets are willing to pay partitioned fees if the fee is viewed as fair. Improving saliency of pet fees is revenue …

Reviving The Christian Left: A Thematic Analysis Of Progressive Christian Identity In American Politics, Adam Blake Arledge

USF Tampa Graduate Theses and Dissertations

While Christianity in American politics today is mostly dominated by the voice of the Religious Right, many are unaware that there also exists a significant number of progressive Christians throughout the country. This diverse group, often referred to as the Christian Left, is not as organized or outspoken as conservative Christians and tends to shy away from the restrictive influence of identity labels. However, members all share in common a passion for social justice issues. This study aims to gain a deeper understanding of the identity of Christian Left individuals through a thematic analysis of interviews. By interrogating participants’ identity …

Communicating Breast Cancer Awareness: Using The Health Belief Model To Develop Mass Communication Themes To Influence Early Detection Behaviors, Srisai Kamakshi Ramya Harika Pucha

USF Tampa Graduate Theses and Dissertations

Breast cancer (BC) is the most fatal cancer in India and has affected more than 1.3 million people in the year 2020 alone, making it a national public health priority. The reason for this alarming increase in BC incidences and mortality rates stems from a severe lack of awareness and the absence of targeted interventions. Considering the absence of a national level breast cancer awareness program, the following study sought to understand Indian women’s preconceived notions about the disease and the available screening methods to contribute to future mass communication campaigns in India. The study used a pre-designed interview guide …

To Be Or Not To Be: A Tale Of Staphylococcal Gpsb, Lauren R. Hammond

USF Tampa Graduate Theses and Dissertations

There are two big drivers motivating studies into bacterial cell division. The first is a desire to understand life around us. One of the defining characteristics of life is the ability for a cell to grow and divide, and without in-depth knowledge of this process, we cannot truly understand the complexities that allow for life on this planet. The second motivator is to identify new drug targets in the ongoing fight against antimicrobial resistance. Many infectious organisms have become resistant to commonly used antibiotics, and the bacterial cell division machinery is a largely untapped essential process with many potential therapeutic …

Lord Of The Z-Rings: Uncovering The Role Of Mraz And Ftsl In Bacillus Subtilis Cell Division, Maria Louise White

USF Tampa Graduate Theses and Dissertations

Cell division is a complex and highly orchestrated process that requires careful coordination. The process of cell division in prokaryotes primarily occurs via binary fission, where one mother cell becomes two genetically identically and equally sized daughter cells. In most bacteria a key protein, FtsZ, forms a FtsZ ring (Z-ring) to mark the division site and recruit proteins involved in cytokinesis. Whilst much has been learned about the contents and assembly of the divisome in model organisms such as Escherichia coli and Bacillus subtilis factors governing the regulation of divisome are much more poorly understood. …

Ec Expression Of Activated K-Ras Leads To Capillary Deficiency Due To Excessive Lumen Formation, Reduced Pericyte Recruitment, And An Increased Susceptibility To Mmp-1-Dependent Vascular Regression, Zheying Sun

USF Tampa Graduate Theses and Dissertations

Activated k-Ras mutations play a role in a variety of pathological conditions, such as arteriovenous malformations (AVMs). To study the effect of activated k-Ras mutation on lumen formation, we engineered human ECs to simultaneously express the activated k-Ras (G12V) mutation and compared their ability to form lumens with control ECs. We demonstrated that ECs with k-RasV12 lead to marked stimulation of EC lumen formation due to dramatic increases in intracellular vacuole formation and fusion, signaling changes including accelerated Src kinase activation, and MT1-MMP-dependent lumen expansion, all of which drive the process of lumen formation. The markedly accentuated lumen formation is …

Identifying Significant Factors Affecting The Likelihood And Severity Level Of Shared E-Scooter Crashes, Recep Can Cakici

USF Tampa Graduate Theses and Dissertations

• Objective: To identify significant factors that affect the possibility of shared e-scooter related crashes in Tampa and St. Petersburg, FL, and also to capture determinants contributing to the level of injury of shared e-scooter related crashes in Tampa, FL.
• Background: Over the past few years, shared e-scooters have become a common form of micromobility; however, safety concerns have been raised due to the increased number of injury accidents involving e-scooters.
• Data and data sources: Survey questionnaires were designed to collect data from the general public on e-scooter sharing programs in Tampa and St. Petersburg, FL. The collected data include respondents’ …

Evaluation Of Response Modality In Online Discussion Boards, Tatiyanna A. Dunn

USF Tampa Graduate Theses and Dissertations

Since 2020, over 3 million students have enrolled in a postsecondary distance learning course (National Center for Education Statistics, 2020). One challenge in distant learning is fostering student engagement. Student engagement is a broad construct that refers to increasing contact between three pillars of education. Students, teachers, and course material. Past research has shown increasing engagement leads to improved student performance and instructor evaluations. Unsurprisingly, promoting student engagement is desirable for both students and instructors (Cobb, 2009). Discussion boards allow student engagement between themselves and Instructors while knowledge can still be tested. Few studies have evaluated the modality of student …

Ambient Athleticism: Politicizing Akira’S Accelerationist Olympiad, Thomas G. Chaplin

USF Tampa Graduate Theses and Dissertations

This thesis politicizes Katsuhiro Otomo’s 1988 animated cyberpunk film, Akira, specifically through how it stages its myriad neoliberal crises as opportunities for accelerationist solutions mediated by the Tokyo Olympics. Akira’s display of fully animated and intense action physics produces an aesthetic relation to its own athletes that contracts around their bodies in an attempt to transgress classical and oppressive compositions. Akira’s vague utopic promise receives broad acceptance and affirmation by extant scholarship, often relying on the accelerative impulses found in the works of Gilles Deleuze to substantiate Akira’s hopeful ending. Invoking Gilles Deleuze’s notion of athleticism, this thesis critically rereads …

An Object For Sexual Pleasure: Does Viewing Sexualized Media Predict Increases In Self And Partner Objectification Impacting Feelings Of Sexual And Romantic Closeness?, Kaitlyn Ligman

USF Tampa Graduate Theses and Dissertations

Exposure to sexually objectifying media has been linked to the objectification of the self and of one’s romantic partner (e.g., partner-objectification); yet the implications of this for romantic relationships have remained relatively unexamined. There is, however, reason to suspect that exposure to sexually objectifying media and engaging in objectification may have implications for romantic couples. When a woman frequently monitors her appearance this may undermine her ability to sexually connect with her partner and when a man views his partner as an object for sexual pleasure it may impede his ability to develop intimate feelings of relational closeness to his …

Organizing For Here And There: Exploring The Grassroots Organizing Of The Puerto Rican Diaspora In The Tampa Bay Area, Dominique Rivera

USF Tampa Graduate Theses and Dissertations

Drawing upon participant observations and semi-structure interviews with 10 Puerto Rican grassroots organizers from the Tampa Bay area of Florida, this project examines the processes by which Puerto Rican diaspora members build, maintain, and utilize social and symbolic ties as resources for organizing and executing grassroot projects and campaigns with a dual focus on the Puerto Rican community in the Tampa Bay area and in Puerto Rico. Complex webs of interlocking social and symbolic ties that transcend region of origin and regions of destination constitute a transnational social field, within which exchanges of ideas, practices, and resources are organized among …

Social Media Time Series Forecasting And User-Level Activity Prediction With Gradient Boosting, Deep Learning, And Data Augmentation, Fred Mubang

USF Tampa Graduate Theses and Dissertations

In the overall history of technological innovations, social media has only existed for a brief time, however its influence is undeniable. Researchers have found that it can be used to influence elections, spread health misinformation, and aid with financial pump-and-dump schemes. Keeping all this in mind, it is clear that more research is needed to predict the spread of information on social media in order to combat its malicious use.

To that end, in this dissertation, we explore the use of Machine Learning algorithms to perform time series forecasting and user-level activity prediction in social media. We address the different …

Queering The Weeki Wachee Mermaid And Its Renewed Aesthetic Value, Jacqueline D. Merveille

USF Tampa Graduate Theses and Dissertations

Scholars have asserted that the visual representation of womanhood responds to a cultural context, which often adheres to patriarchy and the satisfaction of the male gaze. The mediated depiction of the mermaid through time is polysemous and therefore deserves a thorough examination substantiated by robust theories. As a gendered feminine figure that complies with the patriarchal pressure of subjugation to gratify male desire, the mermaid can, however, elude the socially constructed dichotomy of genders, contributing to her agency and queerness. By investigating the Florida roadside attraction of Weeki Wachee Springs’ mermaid shows and other mediated visual depictions of the sea …

Going Flat: Challenging Gender, Stigma, And Cure Through Lesbian Breast Cancer Experience, Beth Gaines

USF Tampa Graduate Theses and Dissertations

This paper explores the decision-making process of reconstruction surgery among lesbian breast cancer patients to better understand how identity impacts healthcare decisions. Breast cancer patients experience the disease in unique ways due to gender, sexuality, race, and class, impacting their individual decisions regarding treatment plans. Many breast cancer patients face mastectomy surgery as the first plan of treatment after diagnosis. By exploring the impact of gender, sexuality, stigma, and ideas of cure, this research aims to advance research about breast cancer by recognizing why some lesbian breast cancer patients forego reconstruction surgery and instead choose to “go flat.

A Holistic Investigation Of Acidosis In Breast Cancer, Bryce Ordway

USF Tampa Graduate Theses and Dissertations

In the early 20th century, Nobel laureate Otto Warburg made the observation that cells of a carcinoma had considerably higher glycolytic metabolism and considerably lower oxidative metabolism compared to cells of a normal tissue. He postulated that within this observation was the key to deciphering the differences between malignant and normal tissue. It is now well established that tumors of the breast are unequivocally acidic, caused by an abnormal amount of aerobic glycolysis, colloquially known as the Warburg effect. Over the last decades, our group, led by Dr. Robert J. Gillies, has set out to characterize the causes and consequences …

Beliefs, Identity, And An African American Cemetery: An Exploratory Study Of Difficult History Curricular Decision- Making, Shannon Peck-Bartle

USF Tampa Graduate Theses and Dissertations

In this qualitative exploratory study, I examine the influence of administrative curricular decision-makers’ beliefs and values towards race and ethnicity, heritage, and place on curricular aims for the inclusion of local difficult history associated with the erasure of a racialized cultural landscape, The Ridgewood Cemetery. I additionally examine the influence of contemporary issues on beliefs and values as administrative curricular decision-makers navigate ways to incorporate local cemetery history into secondary social studies curriculum. Through semi-structured interviews, document analysis, and research’s reflective journaling I shed light on ways beliefs, values, and contemporary issues influenced administrative curricular decision-making for local difficult history. …

A Residents’ Lens To Community Wellbeing, Sarah Combs

USF Tampa Graduate Theses and Dissertations

Despite its location just a few miles from the burgeoning University of South Florida, the University Area Community (UAC) is considered a severely economically disadvantaged neighborhood. When compared to its surrounding counties, the UAC experiences heightened unemployment and poverty rates in conjunction with a significantly lower median income and rate of homeownership. This study utilized a research method of Narrative Inquiry as told through Qualitative Research to gain a better understanding of the UAC residents’ lived experiences and of how community change agents could succeed in impacting community wellbeing in areas like affordable housing, resident employment, and resident engagement. The …

X-Linked Ubiquitin-Specific Peptidase 11 Increases Tauopathy Vulnerability In Women, Yan Yan

USF Tampa Graduate Theses and Dissertations

Women experience significantly higher tau burden and increased risk for Alzheimer’s disease (AD) than men, yet the underlying mechanism for this vulnerability has not been explained. Here, we demonstrate through in vitro and in vivo models, as well as human AD brain tissue, that X-linked ubiquitin specific peptidase 11 (USP11) augments pathological tau aggregation via tau deubiquitination initiated at lysine-281. Removal of ubiquitin provides access for enzymatic tau acetylation at lysines 281 and 274. USP11 escapes complete X-inactivation, and female mice and people both exhibit higher USP11 levels than males. Genetic elimination of usp11 in a tauopathy mouse model preferentially …

Hidden Struggles Within The Mental Healthcare Workplace, Tammy V. Smith

USF Tampa Graduate Theses and Dissertations

For decades, the Centers for Disease Control and Prevention (CDC) and World Health Organization (WHO) have reported on the worldwide pervasiveness and increasing societal costs associated with Adverse Childhood Experiences (ACEs). A recent post on the WHO website cited recent scientific research (2022) indicating the magnitude of harmful effects attributed to ACEs, even classifying them as “violence against children.” Esaki and Larkin (2013) indicate that ACEs are prevalent among many social service professionals, including mental health clinicians.

This study uses mental health clinicians as the sample population to identify the impact of ACEs on job performance. Findings from a volunteer …

Neural And Cardiac Mechanisms In Friedreich's Ataxia With Patient-Derived Ipscs, Mariana Burgos Angulo

USF Tampa Graduate Theses and Dissertations

Friedreich's ataxia (FA) is an autosomal recessive disease caused, in most cases, by a GAA trinucleotide repeat expansion in the first intron of the frataxin (FXN) gene, which results in transcriptional repression of the encoded protein frataxin. FA is a progressive neurodegenerative disorder, but the primary cause of death is hypertrophic cardiomyopathy, which occurs in 60% of the patients. Several functions of frataxin have been proposed, but none of them can fully explain why its deficiency causes the FA phenotypes nor why the most affected cell types are neurons and cardiomyocytes. It is possible that frataxin affects neural and cardiac …

Identification Of Secondary Structural Elements Contained Within The Intrinsically Disordered N-Terminal Tail Of The Bloom’S Syndrome Helicase., Vivek Somasundaram

USF Tampa Graduate Theses and Dissertations

The Bloom’s Syndrome Helicase (BLM) is one of five human RecQ helicases and is necessary for maintenance of genome stability. Whilst a crystal structure exists for the C-terminal domain of BLM, very limited structural knowledge is known about the intrinsically disordered N-terminal tail. This lack of insight exists, despite the fact that the N-terminus of BLM is essential for the overall biological activity of BLM. Here we provide an Nuclear Magnetic Resonance spectroscopy based approach that we used to identify two distinct ⍺-helices contained within the first 100 residues of BLM. In addition, we propose a mutagenesis-based approach involving rationally …

“Good War” On The Cool Medium: The Cold War's Influence On The Portrayal Of World War Ii On American Television, Michael A. Losasso

USF Tampa Graduate Theses and Dissertations

This dissertation is a cultural history of the Cold War’s influence on the portrayal of World War II on American television. In it, I chronicle the production, reception, and presentation of the war of three prominent American-produced documentary series from three periods of the Cold War – Victory at Sea (1952-1953), The Twentieth Century (1957-1966), and The Unknown War (1978). The dissertation posits that from the 1940s-1960s, the television portrayal of World War II was an embodiment of the values- that the United Sates was “fighting” for through a “triumphalist” narrative of a “just war” which recast the role of …

4d Printing Of Smart Hydrogel Scaffold To Program Neural Stem Cell Differentiation, Omar Khater

USF Tampa Graduate Theses and Dissertations

Injuries like Traumatic brain injuries (TBI) and Spinal cord injuries (CSI) areconsidered one of the biggest challenges in the medical sector as there is no specific treatment for these injuries and the medical intervention on these injuries is restricted to reducing the pain and removing the damaged human parts. One of the helpful and most effective treatment methods for these injuries involve neural tissue regeneration in the area of the injury. The main issue when trying to treat such injuries is that neural tissues are often unable to regenerate by themselves and require outer human intervention to differentiate. Biomedical scaffolds …

Elemental Sulfur And Metal Sulfide Minerals For Autotrophic Denitrification: Applications To Aquaculture, Groundwater Treatment, And Domestic Wastewater Treatment, Erica Amah Dasi

USF Tampa Graduate Theses and Dissertations

Nutrient pollution of waterways, from excess N and P, is a global concern that results in harmful impacts the environment, human health, and aquatic animals. This dissertation harnesses sulfur autotrophic denitrification to remove nutrients from groundwater, domestic wastewater, and wastewaters produced from marine recirculating aquaculture systems (RAS). Part 1 evaluates the denitrification performance of woodchips and elemental sulfur in cyclic denitrification filters (WS-CDFs) integrated into marine RAS operated under saline conditions. Part 2 investigates the use of metal sulfide minerals, with and without oyster shells, for supporting nutrient removal from groundwater in batch reactors. Part 3 assesses …

Healthcare Iot System And Network Design, Halil Ibrahim Deniz

USF Tampa Graduate Theses and Dissertations

The developing IoT concept offers many opportunities to service providers in the medical field. However, the functionality of the developed systems is increasing day by day, and it brings many different problems. One of the most important problems is the transmission of biomedical data of real-time monitoring systems to the medical server with the least delay. Network architectures are changing to meet the changing needs of densely connected devices, and “computing at the edge” is the new architectural approach emerging in IoT networks. This architecture is more dynamic than computation in the cloud because it enables data processing at each …

Revisiting ‘Our’ Place On Campus: A Queer(Ed) And In-Depth Interview Study Of Qt Resource Professionals In Higher Education, Kristopher Andrew Oliveira

USF Tampa Graduate Theses and Dissertations

This dissertation offers an interactional, structural, and theoretical analysis of the experiences of Queer and Trans (QT) resource professionals in higher education. Data from the empirical chapters draws on 41 in-depth semi-structured interviews that conducted with QT resource professionals from 19 different states across the United States. The interactional and structural chapters reveal tensions between institutional commitments to doing equity work on college campuses and the neoliberal and institutional barriers that QT resource professionals experience in promoting and fostering institutional equity on campus. The third substantive chapter offers a queer of color critique – through Muñoz’s (2019) notion of queer …

Inflammatory Bowel Disease & Social (In)Visibility: An Interpretive Study Of Food Choice, Self-Blame And Coping In Women Living With Ibd, Jessica N. Lolli

USF Tampa Graduate Theses and Dissertations

This dissertation is an interpretive project that uses autoethnography and qualitative interview methods to understand the role Inflammatory Bowel Disease (IBD) plays in women’s quality of life and interpersonal relationships. It focuses on the impacts on patients of dietary changes and how food choice serves to make this ordinarily “invisible” illness visible to others, leading to unwanted exposure. Using Erving Goffman’s stigma theory and its extensions in studies of chronic illness, I demonstrate that IBD is characterized by layers of stigma because it creates situations in which patients are forced to disclose their illness even if they are not ready …

Digital Realness: Queer Intimacy In Contrapoints, William S. Beaman

USF Tampa Graduate Theses and Dissertations

Digital media comprises a diverse ecology of creative genres, institutions, communities,platforms, and entrepreneurial businesses. Yet despite its practical association with variegated social forms, digital mediation as such is often theorized as a logic of homogenization, problematically obscuring its heterogeneously contested character. This article reconceives mediation as an irreducibly multi-scalar and heterogeneous infrastructure, recasting online activity as contested participation in wider social contexts. I am contributing to a counter tendency in media studies that methodologically treats digital forms as polysemic, ambiguous and contested, rather than necessarily homogenizing, in the context of specific cases. Drawing out the theoretical implications of this methodology …