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Novel Sol-Gel Titania-Based Hybrid Organic-Inorganic Coatings For On-Line Capillary Microextraction Coupled To High-Performance Liquid Chromatography, Tae-Young Kim
USF Tampa Graduate Theses and Dissertations
Novel sol-gel titania-poly(dimethylsiloxane) (TiO2-PDMS) and titania-silica-N-(triethoxysilylpropyl)-O-polyethylene oxide urethane (TiO2-SiO2-TESP-PEO) coatings were developed for capillary microextraction (CME) to perform on-line preconcentration and HPLC analysis of trace impurities in aqueous samples. Due to chemical inertness of titania, effective covalent binding of a suitable organic ligand to its surface is difficult via conventional surface modification methods. In this research, sol-gel chemistry was employed to chemically bind hydroxy-terminated poly(dimethylsiloxane) (PDMS) and N-(triethoxysilylpropyl)-O-polyethylene oxide urethane (TESP-PEO) to sol-gel titania and sol-gel titania-silica network, respectively. A method is presented describing in situ preparation of the titania-based sol-gel PDMS and TESP-PEO coatings and their immobilization on the …
Control Of Lysogeny In Marine Bacteria: Studies With Φhsic And Natural Populations, Amy K. Long
Control Of Lysogeny In Marine Bacteria: Studies With Φhsic And Natural Populations, Amy K. Long
USF Tampa Graduate Theses and Dissertations
Viruses have an estimated global population size of 10 to the 31st, with a significant proportion found in the marine environment. Viral lysis of bacteria affects the flow of carbon in the marine microbial food web, but the effects of lysogeny on marine microbial ecology are largely unknown. In this thesis, factors that influence the control of lysogeny were studied in both the phiHSIC/Listonella pelagia phage-host system and in bacterioplankton populations in the Gulf of Mexico. Using macroarrays dotted with phiHSIC amplicons, viral gene expression over the course of a synchronous infection experiment was measured. Early, middle, late, and continually …
Gender Differences During Heat Strain At Critical Wbgt, Christina L. Luecke
Gender Differences During Heat Strain At Critical Wbgt, Christina L. Luecke
USF Tampa Graduate Theses and Dissertations
Heat stress is influenced by environmental conditions, workload and clothing. A critical environment is the upper limit of compensable heat stress for a given metabolic rate and clothing ensemble. The physiological strains associated with heat stress are core and skin temperatures, heart rate and physiological strain index (PSI). Because heat dissipation mechanisms may differ between men and women, there may be gender differences in the critical environment and the associated physiological variables. Gender differences were explored between acclimated men (n = 20) and women (n = 9) at the upper limit of compensable heat stress. Participants walked on a motorized …
Audience Interpretations Of The Representation Of Women In Music Videos By Women Artists, Libby Mckenna
Audience Interpretations Of The Representation Of Women In Music Videos By Women Artists, Libby Mckenna
USF Tampa Graduate Theses and Dissertations
This study examined how young women audiences may make sense of music videos and how they relate music video messages to their own femininity. Studies of the representation of women in music videos mostly have focused on critiquing content, rather than audience interpretations. This study, using focus groups, looked at how young women interpret music videos featuring women artists and showed that young women perceive sexist and/or stereotypical depictions presented in the videos. During six focus groups, three music videos were reviewed by 49 college students, mostly consisting of young women in their early 20s. The researcher found themes emerging …
Study Of The Ethical Values Of College Students, Victor Mercader
Study Of The Ethical Values Of College Students, Victor Mercader
USF Tampa Graduate Theses and Dissertations
This study focuses on five main purposes, all of them interrelated and each focused on ethical values, virtues, or character values. The five purposes are: a) Investigate college students' perceptions of ethical values, including their importance, application, usefulness, origin, benefits, need for education, and courses proposed to be included in the curricula; b) Review literature in areas related to ethical values, virtues or character values of college students; c) Develop and pilot an instrument to assess the ethical values of college students; d) Improve and use the developed instrument to describe the status of college students' ethical values; and e) …
Developing Culture Conditions To Study Keratocyte Phenotypes In Vitro, Kurt Musselmann
Developing Culture Conditions To Study Keratocyte Phenotypes In Vitro, Kurt Musselmann
USF Tampa Graduate Theses and Dissertations
The corneal wound healing response involves the activation of keratocytes to proliferate from a quiescent phenotype. The mitogens that cause the initial transformation of the quiescent keratocytes to the active phenotype have not been identified. Even though serum is commonly used to replicate this in vitro, the cornea is avascular and therefore likely not exposed to serum. In the first part of this dissertation, a DMEM/F12 extract of corneal stromas was made and shown to stimulate keratocyte proliferation in both a dose-dependent and cell-density dependent manner. This extract contains mitogens that differ from the mitogens present in serum based on …
Altered Features Of Female Pigeons (Columba Livia) Elicit Preference Behavior In Male Pigeons, Tadd B. Patton
Altered Features Of Female Pigeons (Columba Livia) Elicit Preference Behavior In Male Pigeons, Tadd B. Patton
USF Tampa Graduate Theses and Dissertations
Previous research has shown that male pigeons (Columba livia) respond with courtship displays to video playbacks of a female pigeon indicating that they 'recognize' the female as a potential mate. Courtship displays significantly decline when the head region of the female is occluded (Shimizu, 1998) suggesting that features located within the head are important for species recognition and mate selection. However, little is known about the exact visual features necessary to elicit displays. The current study examined the preference behavior of male pigeons when given a choice between photographic images of normal looking or altered female pigeon faces. The altered-face …
Clarifying The Cohesion-Performance Relationship In Teams: Backup Behavior As A Mediating Mechanism, Matthew S. Prewett
Clarifying The Cohesion-Performance Relationship In Teams: Backup Behavior As A Mediating Mechanism, Matthew S. Prewett
USF Tampa Graduate Theses and Dissertations
The relationship between team cohesion and team performance has been extensively studied, but behavioral mediators of this relationship have not been adequately assessed. This study proposed that backup behavior mediates the relationship between team cohesion and team performance. In addition, it was also hypothesized that team goal commitment would moderate the relationship between team cohesion and team backup behavior. 138 participants forming 46 teams of three were assigned to one of fours conditions to test this framework: high cohesion/high goal commitment, high cohesion/low goal commitment, low cohesion/high goal commitment, and low cohesion/low goal commitment. Results indicated no significant differences between …
Women In Nontraditional Careers, Teresa Ann Roche
Women In Nontraditional Careers, Teresa Ann Roche
USF Tampa Graduate Theses and Dissertations
In a traditional male dominated field such as construction, and automotive technology, artificial barriers and attitudes have often prevented qualified females from reaching their full potential. The late entrance of females into these fields has created very few role models for nontraditional younger females entering these professions. This study was designed to create a profile for nontraditional females working in a male dominated work force. A large percentage of females have experienced some barriers due to discrepancies in gender performance. Acceptance by peers, community and administrators, combined with the challenge of balancing family and career appears to be areas of …
Spin Polarization Measurements And Sensor Applications In Thin Films And Carbon Nanotube-Based Devices, Jeff T. Sanders
Spin Polarization Measurements And Sensor Applications In Thin Films And Carbon Nanotube-Based Devices, Jeff T. Sanders
USF Tampa Graduate Theses and Dissertations
The unique properties of carbon nanotubes (CNTs) show a great deal of potential for nanoelectronic devices, spintronic devices, biosensing and chemical sensing applications. Their applicability as interconnects for spintronic devices derives from their one-dimensionality and theoretically predicted preservation of spin current. In this work, we combine an investigation of spin polarization in materials such as half metallic oxides in thin film and bulk form with studies on several aspects of CNTs for sensing and spin transport applications. These two areas of study are intimately related within the umbrella of spin-electronics and nanoscale sensors that are being pursued with great topical …
An Examination Of The Interaction Between Exemplary Teachers And Struggling Writers, Betty Ruth Sylvester
An Examination Of The Interaction Between Exemplary Teachers And Struggling Writers, Betty Ruth Sylvester
USF Tampa Graduate Theses and Dissertations
This study examined the interactions between teachers of writing and struggling writers. There were two main research questions: (1) What is the nature of the interaction between exemplary teachers of writing and struggling writers? (2) What arethe responses of struggling writers to exemplary teachers' scaffolding? To answer these questions, qualitative analysis was conducted on data. Two struggling writers were selected for the study based on their responses to the Writers Self-Perception Scale,writing samples, and teacher recommendation. Data collection included observation in two separate fourth grade classrooms during the writing block for 30 days. Data sources included audio-recording of writing instruction …
A Descriptive Study Of Students’ Perspectives On Controversial Issues Embedded In A College Environmental Science Course, Chyrisse P. Tabone
A Descriptive Study Of Students’ Perspectives On Controversial Issues Embedded In A College Environmental Science Course, Chyrisse P. Tabone
USF Tampa Graduate Theses and Dissertations
This qualitative study described non-science undergraduate majors' responses to controversial issues embedded in an introductory level environmental science course in a liberal arts college located in the southeastern United States. Participants enrolled in this 12-week summer course were both traditional college-age (late teens to early twenties) and non-traditional age student (thirties to fifties). Approximately 76 percent were female. Students demonstrated various lifestyles (e.g., gay, single-parent, living at home), socioeconomic statuses (e.g., middle-income, low income), employment (e.g., employed, unemployed, ex-military) and ethnicities. The structure of the environmental science course was consistent with the science education reform movement standards applied to K-12 …
Ethnic Differences In Body Mass Index, Christine Anne Vaughan
Ethnic Differences In Body Mass Index, Christine Anne Vaughan
USF Tampa Graduate Theses and Dissertations
The greater body mass of African American females relative to Caucasian females is a well-documented finding implicated in ethnic disparities in health outcomes. The principal aim of the current study was to evaluate a theoretical account that may explain ethnic differences in body mass index. The proposed theoretical account focused on appearance-related concerns regarding the desirability of a thin body type as motivation to engage in weight control behavior. It was hypothesized that Caucasians would evidence greater internalization of the thin ideal than African Americans, which would then be associated with greater dietary restriction and physical activity, thereby predicting lower …
Development Of Ganglioside-Based Assays For The Identification Of Botulinum And Cholera Toxins Utilizing An Evanescent Wave Biosensor, Crystal M. Bedenbaugh
Development Of Ganglioside-Based Assays For The Identification Of Botulinum And Cholera Toxins Utilizing An Evanescent Wave Biosensor, Crystal M. Bedenbaugh
USF Tampa Graduate Theses and Dissertations
An evanescent wave fiber-optic biosensor was used in an effort to develop an assay for the rapid detection of two biological toxins: cholera toxin and botulinum toxin. The Analyte 2000 fiber-optic biosensor utilizes a sandwich immunoassay format. Gangliosides or liposomes are directly adsorbed to the surface of the fiber-optic waveguide through hydrophobic interactions. The waveguide is exposed to a sample containing the toxin of interest, then subsequently exposed to a polyclonal detection antibody conjugated to the fluorophore cyanine 5. Excitation light from a 635nm laser diode is propagated through the waveguide and fluorescent molecules within approximately 100nm of the waveguide …
Community Of Practice As Community Of Learners: How Foreign Language Teachers Understand Professional And Language Identities, Ruth Ban
USF Tampa Graduate Theses and Dissertations
This study seeks to understand the transmogrification of four Mexican foreign language teachers as they participate in a teaching exchange in American schools throughout the United States. Previous research into development of competent membership in given communities of practice points to the need for mutual engagement with other community members, an understanding of the community activity, and a shared repertoire among community members. Framed within an activity theory perspective, the present study examined how the teachers's socio-historical background, cultural tools and artifacts, along with other pedagogical activities, community rules, and division of labor mediated the teachers' understanding of their professional …
School Principals’ Knowledge And Understanding Of Educator Sexual Misconduct Against Students, Sylvia Sonja Cairns
School Principals’ Knowledge And Understanding Of Educator Sexual Misconduct Against Students, Sylvia Sonja Cairns
USF Tampa Graduate Theses and Dissertations
The purpose of this research study was to explore what public school principals know and understand about educator sexual misconduct. This project attempted to provide a clearer picture of how administrators perceived and performed their leadership role as moral keeper of the school. In examining the literature, the researcher determined that there is a paucity of information concerning educator sexual misconduct.To accomplish the goals of this research project, the investigator paid particular attention to the study, Educator Sexual Misconduct: A Synthesis of Existing Literature, prepared by Shakeshaft in 2004 for the U.S. Department of Education. Insights afforded by Shakeshaft's study …
Bacteriophage For The Elimination Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Colonization And Infection, Angela Clem
Bacteriophage For The Elimination Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Colonization And Infection, Angela Clem
USF Tampa Graduate Theses and Dissertations
Methicillin-resistant Staphylococcus aureus (MRSA) is among the most important pathogens affecting the human race in our time. In spite of recent medical advances, our therapeutic choices for MRSA infections remain limited due to the propensity of this organism to develop resistance to antimicrobials. Therefore, there is a continuing need to develop newer methods of treating MRSA infections. This dissertation examines the effects of bacteriophages 88 and 92 on ten clinical isolates of MRSA from the central Florida area. . For the majority of the MRSA isolates, bacteriophages 88 and 92 were unable to induce lysis. However, bacteriophage 88 was found …
The Commodification Of Yoga In Contemporary U.S. Culture, Michelle E. Demeter
The Commodification Of Yoga In Contemporary U.S. Culture, Michelle E. Demeter
USF Tampa Graduate Theses and Dissertations
Yoga is an increasingly visible and versatile commodity in the United States health market. Though its origins stretch to pre-Vedic India and its traditional religious purpose is linked to Hinduism, it is evident that yoga has undergone much change since its transmission to U.S. culture. In its popular, widespread incarnation in the United States, yoga is not usually learned at the feet of a guru, but at exercise centers and gyms. These secular locales of yoga's practice help define the "yoga phenomenon" in contemporary America. This phenomenon has resulted in yoga's wide acceptance and high visibility in American popular culture …
Rapid Detection Of Mycobacterium Tuberculosis In Lung Tissue Using A Fiber Optic Biosensor, Kimberly A. Denton
Rapid Detection Of Mycobacterium Tuberculosis In Lung Tissue Using A Fiber Optic Biosensor, Kimberly A. Denton
USF Tampa Graduate Theses and Dissertations
There is no rapid diagnostic technique at medical examiners' offices to determine if a decedent is infected with Mycobacterium tuberculosis. Present diagnostic testing requires at least one month for results. A biosensor-based sandwich immunoassay for the detection of M. tuberculosis was developed in this study. M. tuberculosis polyclonal antibody was used for target antigen capture and detection in the immunoassay. Live attenuated M. tuberculosis (ATCC 25177) suspended in phosphate-buffered saline with 0.1% Tween 20 was used as the antigen in the detection assay. The Analyte 2000 was the initial biosensor platform. Initial testing was of Freund's adjuvant complete. M. tuberculosis …
An Examination Of The Experiences Of Five African American Male Students With Regard To School Discipline Practices, Simon Yohann Earle
An Examination Of The Experiences Of Five African American Male Students With Regard To School Discipline Practices, Simon Yohann Earle
USF Tampa Graduate Theses and Dissertations
There are numerous studies that address the issue of discipline practices in schools. Within the last decade there have been an increasing number of scholars and school district personnel who have written about discipline, and more specifically, the discipline of specific populations of students such as African Americans. Although many of these studies address the disparate disciplinary practices that occur in many school districts, little attention is devoted to how students understand these practices in the larger context of their general school experiences. This research intends to fill the void in the literature that utilizes the experiences of students to …
Asymptotes And Metaphors: Teaching Feminist Theory, Michael Eugene Gipson
Asymptotes And Metaphors: Teaching Feminist Theory, Michael Eugene Gipson
USF Tampa Graduate Theses and Dissertations
As we move through our daily lives, the cogency of the world shifts and changes. Many constructs exist to explain and account for how we view and interact with our environment. Education is where our understandings become formalized and are challenged. To this end, a plethora of pedagogical tools are made available to aid educators in illuminating the world(s) around and within each student. However, there is always room for new ways of presenting information, concepts, and ideas. I put forth the mathematical trope of asymptotes as a new pedagogical tool. Asymptotes, as metaphor, work as a pedagogical tool for …
A Marriage Of Convenience: Batista And The Communists, 1933 – 1944, Charles Clayton Hollenkamp
A Marriage Of Convenience: Batista And The Communists, 1933 – 1944, Charles Clayton Hollenkamp
USF Tampa Graduate Theses and Dissertations
This paper examines the relationship between Fulgencio Batista and the Communist Party of Cuba. At odds during the first several years of Batista's rule, when strikes and repression were the topics of the day, the two sides eventually saw in each other a means to an end. In efforts to understand the Cuban Revolution of the late 1950's, historians often portray Batista as a dictatorial puppet of American business and policy. Contrary to this image, in his first regime (1934 until 1944), Batista presided over the creation of a nominal constitutional democracy. To do this he needed the support and …
A New Approach To Kainoids: Total Syntheses Of (-)-Kainic Acid And (+)-Allokainic Acid, Young Chun Jung
A New Approach To Kainoids: Total Syntheses Of (-)-Kainic Acid And (+)-Allokainic Acid, Young Chun Jung
USF Tampa Graduate Theses and Dissertations
(-)-Kainic acid and its C-4 epimer, (+)-allokainic acid are parent members of a class of substituted pyrrolidines known as kainoids. They have been found to exhibit powerful biological properties, principally neuroexcitatory. Kainic acid has become especially important in the study of Alzheimer's disease, epilepsy, and other neurological disorders. The total syntheses of (-)-kainic acid and (+)-allokainic acid were achieved using (L)-glutamic acid as the starting material and the sole source of stereochemical induction. The key steps for these successful syntheses involve formation of the gamma-lactam core via rhodium (II) catalyzed intramolecular C-H insertion of the alpha-diazo-alpha-(phenylsulfonyl)acetamide intermediate and the stereoselective …
Mathematical Model Of Arsenic Adsorption In A Modified Zeolite / Microfiltration System, Miles B. Beamguard
Mathematical Model Of Arsenic Adsorption In A Modified Zeolite / Microfiltration System, Miles B. Beamguard
USF Tampa Graduate Theses and Dissertations
Carcinogenic health concerns over arsenic in drinking water caused the USEPA to reduce the maximum contaminant level (MCL) from 50 to 10 ppb, effective on January 23, 2006. This has forced many smaller utilities into expensive treatment or discontinuation of water distribution. Researchers throughout the world are working to develop an inexpensive method for arsenic removal to meet this MCL. Aluminum silicates, or zeolites, are naturally occurring ionic sorbents. Modification of a zeolite may enhance adsorption capacities and ion selectivity. This research investigates the arsenic adsorption capacities of a modified Chabazite. This adsorption, coupled with a hollow fiber, microfiltration membrane …
Arts In New Directions The Development And Application Of A Construct That Uses The Arts To Promote Transformation And Self-Actualization In Health Care And Education/Therapy, Alexxis Avalon
USF Tampa Graduate Theses and Dissertations
Artistic methods to evoke relaxation, spark creativity, and change self-perceptions are already being used by therapists, educators, and scientists. Interdisciplinary collaborations among researchers are developing to create new paradigms that incorporate the use of arts to empower individuals. By describing various connections between the arts and participants, researchers are looking at the expressive arts (including dance, music, drama, poetry, and visual arts) for transformation and self-actualization.The problem is that no construct is available to describe the transformation, "the conscious move from one deep structure of knowledge to another" (Wilber, 2004), that occurs when using the arts in health care and …
Adolescent Alcohol Use And Educational Outcomes, Wesley A. Austin
Adolescent Alcohol Use And Educational Outcomes, Wesley A. Austin
USF Tampa Graduate Theses and Dissertations
There is some controversy over whether adolescent alcohol use has deleterious causal effects on educational outcomes. In particular, does drinking reduce academic performance and school enrollment rates and increase truancy, or does the observed negative correlation between drinking and educational outcomes merely reflect common unobservable factors? This dissertation sheds further light on the issue by estimating the causal impacts of alcohol use on various educational outcomes. Specifically, an instrumental variables model is estimated to study the effects of several drinking measures on grades, school enrollment and absenteeism.
Folk Wiki: The Shared Traditions Of Folk Music And The Wiki Way, Phillip Mark Chamberlin
Folk Wiki: The Shared Traditions Of Folk Music And The Wiki Way, Phillip Mark Chamberlin
USF Tampa Graduate Theses and Dissertations
Wiki is often perceived as representing a revolutionary break from conventional notions of authorship, writing, and textual history. Dialogues concerning Wiki tend to ignore the characteristics that Wiki shares with earlier forms of collaboration, particularly folk music. In both Wiki and folk music, content is often collectively shared and authored, even if specific individuals create and change the content. Many collaborators are anonymous, quasi-anonymous, or pseudo-anonymous, but the perception of this anonymity is, in both genres, problematic. Second, both Wiki documents and folk songs exist in the "Eternal Now," a seemingly perpetual state that makes these texts available for addition, …
The Influence Of Performance Background On Instrumentalists’ Ability To Discriminate And Label Cornet And Trumpet Timbre, Gary Compton
The Influence Of Performance Background On Instrumentalists’ Ability To Discriminate And Label Cornet And Trumpet Timbre, Gary Compton
USF Tampa Graduate Theses and Dissertations
This study was an examination of timbre discrimination and labeling, with specific consideration of the soprano-brass timbres of cornets and trumpets. This study sought to determine whether instrumentalists differed in their ability to label and discriminate cornet and trumpet timbres, and whether these abilities were influenced by the instrumentalists' performing instrument and years of playing experience. Wind, brass and percussion players (n=100) from existing instrumental groups in the Tampa Bay area participated in the study. These groups were collegiate trumpet majors from a large public university school of music, a collegiate wind ensemble from a private university, and a brass …
Drug Resistant Patterns Of Invasive Streptococcus Pneumoniae Infections In The State Of Florida In 2003, Michael T. Drennon
Drug Resistant Patterns Of Invasive Streptococcus Pneumoniae Infections In The State Of Florida In 2003, Michael T. Drennon
USF Tampa Graduate Theses and Dissertations
Streptococcus pneumoniae is a major bacterial pathogen which causes pneumoniae, meningitis, otitis media, and bacteremia. Currently there are two vaccines available, Pneumococcal Polysaccharide Vaccine (PPV) for adults and the Pneumococcal Conjugate Vaccine (PCV) for children. The PCV vaccine was developed in 2000 specifically for children and infants due to the ineffectiveness of the PPV vaccine in children. This is a cross sectional study of invasive S. pneumoniae in Florida during 2003. This study is designed to determine the population characteristics, clinically relevant antibiotic resistance patterns and specific risk factors for development of antibiotic resistance of invasive S. pneumoniae. Participants for …
Optical Imaging Of Radiolabeled Drugs In Tissue Sections Using The Microimager, Paul Dungel
Optical Imaging Of Radiolabeled Drugs In Tissue Sections Using The Microimager, Paul Dungel
USF Tampa Graduate Theses and Dissertations
The MicroImager is a fast, high resolution, real time, digital autoradiographic imaging tool with broad applications. This study utilizes the MicroImager to evaluate radiolabeled drug behavior in subcutaneous tissue. Experiments were conducted in conjunction with mathematical models to determine the diffusion coefficient (D) and elimination constant (k) for radiolabeled dexamethasone. Osmotic pumps containing [3H]dexamethasone were implanted into rat subcutaneous tissue over 6h, 24 h, and 60 h. Local tissue was explanted and slides were prepared for imaging. The MicroImager was then used to quantify the local concentration of 3H-dexamethasone in the tissue surrounding the tip of the osmotic pump. Betavision+ …