Digital Commons Network^™

Optimizing Peptide Fractionation To Maximize Content In Cancer Proteomics, Victoria Izumi

USF Tampa Graduate Theses and Dissertations

The purpose of the studies included in this thesis is to develop an effective an efficient method to study the proteome using separation and detection of peptides, when only a limited amount of sample, 10 micrograms of total protein or less, is available. The analysis will be applied to multiple myeloma cancer cells using ultra high-performance liquid chromatography-mass spectrometry for expression proteomics to illustrate utility. To detect low abundance peptides in a complex proteome, we use different strategies, including basic pH reversed-phase liquid chromatography (bRPLC), mass-to-charge fractionation in the mass spectrometer, and various liquid chromatography gradients to increase peptide separation …

Validation Of The Thermal Work Limit (Twl) Against Known Heat Stress Exposures, Danielle L. Kapanowski

USF Tampa Graduate Theses and Dissertations

Workers are exposed to stressful thermal work environments in multiple industries every day. Methods for assessing heat stress often struggle to balance productivity without compromising the health of the workers. The Thermal Work Limit (TWL) is a method that has been adopted in areas outside of the United States as a viable method for heat assessment that combines health with productivity. TWL recommends a maximum metabolic rate for a given set of environmental conditions, clothing ensemble and acclimatization state. The purpose of this paper was to evaluate the validity of the TWL against a set of heat stress data known …

Remote Estimation Of Surface Water PCo2 In The Gulf Of Mexico, Shuangling Chen

USF Tampa Graduate Theses and Dissertations

Surface ocean partial pressure of CO₂ (pCO₂) is a critical parameter in the quantification of air-sea CO₂ flux, which further plays an important role in quantifying the global carbon budget and understanding ocean acidification. The demand for a clearer understanding of how, and how fast, the ocean is changing due to atmospheric CO₂ absorption, requires accurate and synoptic estimation of surface pCO₂.

Surface ocean pCO₂ is mainly controlled by four oceanic processes – thermodynamics, ocean mixing, biological activities, and air-sea CO₂ exchange. Surface ocean pCO_{2 …}

Ice And Guano Deposits In El Malpais Lava Tubes: Potential Paleoclimate Archives For The Southwest United States, Dylan S. Parmenter

USF Tampa Graduate Theses and Dissertations

Three ice cores and one guano core were obtained from lava tubes in El Malpais National Monument, New Mexico, in the Southwest United States. A large hiatus in the Bat Cave guano record, resulting from mining activities in the early 1900's, left us with only ~50 years of data (from AD 1955-2006) to use in analysis. 13C values in guano (-18 to -11.6‰) primarily fall within the range of C4 plants, and likely indicate a tendency of Tadarida brasiliensis to feed on insects over grasslands to the North or South of the park. Variations in 13C values for this period …

Growth Options And Corporate Goodness, Linh Thompson

USF Tampa Graduate Theses and Dissertations

I find evidence to support the negative impact of growth options on corporate social responsibility (CSR). I propose that attention-constrained managers reduce corporate goodness to focus on growth opportunities. The effect is more pronounced for well-governed firms, for financially-constrained firms, and for capital-intensive social dimensions. Firms reduce their research and development (R&D) and capital expenditures, and experience lower annual buy-and-hold abnormal returns (BHAR) subsequent to significant increases in their social performance. I also report value implications of CSR investments. The empirical evidence suggests that managerial choices to divert attention from growth projects toward CSR hurt shareholders.

Morphodynamics Of Shell Key And Mullet Key Barrier Islands: Their Origin And Development, Zachary J. Westfall

USF Tampa Graduate Theses and Dissertations

Shell Key and Mullet Key are two sandy barrier islands on the West Central Florida coast near the mouth of Tampa Bay. These islands are part of an interconnected barrier-inlet system that includes Pass-a-Grille (PAG) and Bunces Pass. Shell Key is a relatively new island about 40-years of age that formed in between the two inlets of Bunces Pass and PAG. Mullet Key is an island to the south of Shell Key situated between Bunces Pass and the main Tampa Bay channel that has demonstrated large scale upward shoaling events. Using numerical modeling, the wave and tidal conditions at the …

An Investigation Of Habitat Suitability Factors And Their Interactions For Predicting Gopher Tortoise Habitat, Abigail V. Lavallin

USF Tampa Graduate Theses and Dissertations

This thesis evaluates the interaction between four habitat factors vital to the gopher tortoise in Florida. Federally and state listed as threatened throughout its entire range, the gopher tortoise is vital to protect, not only for itself individually but its burrows provide an essential habitat to over 300 species making it a key stone species within its environment. Historic habitat modeling methods are reviewed for the gopher tortoise to highlight the gap on this topic. This research expanded on the methods utilized by Baskaran et al. (2006) evaluating the soil, landcover, percentage of canopy cover and the depth to water …

Statistical Monitoring Of Queuing Networks, Yaren Bilge Kaya

USF Tampa Graduate Theses and Dissertations

Queuing systems are important parts of our daily lives and to keep their operations at an efficient level they need to be monitored by using queuing Performance Metrics, such as average queue lengths and average waiting times. On the other hand queue lengths and waiting times are generally random variables and their distributions depend on different properties like arrival rates, service times, number of servers. We focused on detecting the change in service rates in this report. Therefore, we monitored queues by using Cumulative Sum(CUSUM) charts based on likelihood ratios and compared the Average Run Length values of different service …

Recovery Of Nutrients From Anaerobically Digested Enhanced Biological Phosphorus Removal (Ebpr) Sludge Through Struvite Precipitation, Maraida Balaguer-Barbosa

USF Tampa Graduate Theses and Dissertations

Water resources in Florida have been severely degraded by eutrophic conditions, resulting toxic algae blooms, which negatively affect health and tourism. Eutrophication, or excessive amount of phosphorus (P) and nitrogen (N) in water, overstimulates the production of aquatic plants, depletes dissolved oxygen, and deteriorates the aquatic environment. However, phosphorus is a non-renewable resource essential for all living organisms. In fact, more than half of the total demand for P globally is to supply the food industry, which has concerningly accelerated the depletion rates of phosphate reserves.

In many wastewater treatment plants (WWTPs), the enhanced biological phosphorus removal (EBPR) approach has …

Access To Safe Water Supply: Management Of Catchment For The Protection Of Source Water In Ghana, Michael K. Eduful

USF Tampa Graduate Theses and Dissertations

This study investigates provisions made within institutional and regulatory frameworks of water resources management to enhance multi-stakeholder relationships and the challenges of maintaining those relationships, and implications of water resources management for rural communities in the Densu River basin, Ghana. The primary objectives of this study were four fold, these are to: i) review the existing regulatory framework and how it promotes or hinders multi-stakeholder relationships within the catchment area; ii) examine multi-stakeholder relationships to identify challenges in promoting effective collaboration in water resources management; iii) explore the impacts of catchment management on the livelihoods of rural communities; and iv) …

Emotion Recognition Using Deep Convolutional Neural Network With Large Scale Physiological Data, Astha Sharma

USF Tampa Graduate Theses and Dissertations

Classification of emotions plays a very important role in affective computing and has real-world applications in fields as diverse as entertainment, medical, defense, retail, and education. These applications include video games, virtual reality, pain recognition, lie detection, classification of Autistic Spectrum Disorder (ASD), analysis of stress levels, and determining attention levels. This vast range of applications motivated us to study automatic emotion recognition which can be done by using facial expression, speech, and physiological data.

A person’s physiological signals such are heart rate, and blood pressure are deeply linked with their emotional states and can be used to identify a …

Social Media Deployment In A Business To Business Environment: Theory And Practice., Loran Jarrett

USF Tampa Graduate Theses and Dissertations

Social media is increasingly being used by business-to-business (B2B) firms to engage with their customers as they seek to maintain and grow their relationship with their customers. In my dissertation, I examine what communication objectives B2B firms seek to achieve via social media at different stages of the relationship cycle (exploration, expansion, maturity). I then use a panel of social media experts to evaluate the social media efforts of these firms to determine how well these firms achieve their social media objectives. I then contrast how the social media communication objectives of these firms at different stages of the relationship …

Effect Of Heating On Heat Capacity Of Vegetable Oils Used For Biofuel Production, Sailee Nitin Nagpurkar

USF Tampa Graduate Theses and Dissertations

Biodiesel has become a major renewable fuel for diesel engines particularly from various waste sources. New biodiesel production technologies are emerging with data demands for efficient process design. This project highlights the data requirements, gaps in the thermo-physical property data, and consolidation of experimental and estimation methods for effective process development. Knowing the thermodynamic properties of a substance is important in order to know what process or procedures it has to go under. The aim of this project is to assess the potential of vegetable oils to serve as feedstock for biofuel production by determining their heat capacity (Cp). Such …

Drone Movement Control Using Gesture Recognition From Wearable Devices, Venkata Sireesha Dadi

USF Tampa Graduate Theses and Dissertations

Gesture Recognition is a new and upcoming trend that is being widely used in wearable devices and mobile handheld devices. The sensors like Accelerometer, Gyroscope, Heart rate monitor, Barometer and Ambient Light are mostly being included within the device to detect the static or continuous motion, rotational velocity, heartbeat rate of the user, pressure and light conditions for the device respectively. Implementing algorithms to capture the readings of these sensors and implementing them in a necessary way allows a user to use the wearable devices for a wide variety of applications. One such application is controlling Drone that takes user …

Banking On Blockchain: A Grounded Theory Study Of The Innovation Evaluation Process, Priya D. Dozier

USF Tampa Graduate Theses and Dissertations

Blockchain technology emerged as the underlying database structure for Bitcoin, a cryptocurrency, in 2008. Interest in blockchain by the Financial Services industry grew because of its purported transparency, security, and trust elements which resulted in over $240 million in venture capital funds raised by blockchain firms mostly from banks in the first half of 2017.

The purpose of this study was to examine the underlying innovation evaluation process that Financial Services organizations employed as they evaluated blockchain technology. In this research study, blockchain was considered a technology innovation, which drove the research question: “How do financial services organizations evaluate blockchain …

Money Matters: An Examination Of Special Education Characteristics In Efficient And Inefficient Texas School Districts, Pakethia Harris

USF Tampa Graduate Theses and Dissertations

This study veers from the traditional perspective of examining school efficiency or productivity as a cost minimizing process, in which educational inputs are minimized to achieve maximum outputs (student performance). Instead, it provides a critical examination of the dominant, cost minimizing assumption associated with efficiency models and suggest schools instead behave similarly to budget maximizers as presented in Niskanen’s (1971) seminal budget maximizing framework. The study examines the relationship between total student expenditures and subsequent student outcomes, establishing the relative efficiency of Texas school districts using stochastic frontier analysis within a budget-maximizing framework. Additionally, the study investigates how special education …

Groups Generated By Automata Arising From Transformations Of The Boundaries Of Rooted Trees, Elsayed Ahmed

USF Tampa Graduate Theses and Dissertations

In this dissertation we study groups of automorphisms of rooted trees arising from the transformations of the boundaries of these trees. The boundary of every regular rooted tree can be endowed with various algebraic structures. The transformations of these algebraic structures under certain conditions induce endomorphisms or automorphisms of the tree itself that can be described using the language of Mealy automata. This connection can be used to study boundarytransformations using the propertiesof the induced endomorphisms, or vice versa.

We concentrate on two ways to interpret the boundary of the rooted d-regular tree. In the first approach discussed in detail …

Application Of Image Recognition Technology To Foraminiferal Assemblage Analyses, Christian Helmut Gfatter

USF Tampa Graduate Theses and Dissertations

Analyses of foraminiferal assemblages involve time consuming microscopic assessment of sediment samples. Image recognition software, which systematically matches features within sample images against an image library, is widely used in contexts ranging from law enforcement to medical research. At present, scientific applications such as identification of specimens in plankton samples utilize flow through systems in which samples are suspended in liquid and pass through a beam of light where the images are captured using transmitted light. Identification of foraminifers generally utilizes reflected light, because most shells are relatively opaque.

My goal was to design and test a protocol to directly …

Development Of Bioactive Peptidomimetics, Fengyu She

USF Tampa Graduate Theses and Dissertations

Peptidomimetics are synthetic foldamers that expected more resistant to proteolytic degradation and enormous chemodiversity when compared with peptides. To date, the functional peptidomimetics such as β-peptides, peptoids, oligoureas, etc have been developed in many science fields. In order to explore the unnatural foldameric architectures, it’s necessary to discover the novel frameworks and molecular scaffolds. γ-AApeptides were reported to be a new class of peptidomimetics that showed its potential applications in drug discovery and chemical biology. However, a wide function and property of γ-AApeptides need to be further explored. To expand the potential application of γ-AApeptides in biochemistry, I have been …

A Fundamental Investigation Into Low Thermal Conductivity P-Type Chalcogenides And Skutterudites With Potential Thermoelectric Applications, Dean Hobbis

USF Tampa Graduate Theses and Dissertations

Sustainable and renewable energy is an incredibly important area in today’s society and investigation into avenues to improve this wide ranging technology are underway in many different fields. Thermoelectric devices possess the ability for the direct solid-state interconversion of heat and electrical power, which not only allows for sustainable refrigeration but also waste heat recovery. One current restriction on the efficiency of thermoelectric devices is the disparity in thermoelectric performance of p-type and n-type materials. Furthermore, a key physical attribute shared by the majority of high performing thermoelectric materials is low thermal conductivity. Thus in this thesis three separate p-type …

Essays On Family-Friendly Policies, Child Planning And Children’S Early-Age Outcomes, Stefani Milovanska-Farrington

USF Tampa Graduate Theses and Dissertations

This dissertation consists of three chapters which examine family-friendly reforms, child planning or children’s early-age outcomes. The following are the titles of the chapters of the present dissertation:

• CHAPTER 1: Are Parents and Grandparents Substitutes or Complements? The Effect of Parental and Grandparental Supervision Time Investment on Children’s Early-Age Development.
• CHAPTER 2: The Effect of Family Welfare Support on the Likelihood of Having Another Child and Parents’ Labor Supply.
• CHAPTER 3: The Effect of Maternity Leave Expansions on Fertility Intentions: Evidence from Switzerland.

Chapter 1 uses evidence from Scotland to examine the effect of grandparents’ childcare provision relative to the …

Social And Emotional Learning And Student Achievement In A Culture Of Care, Odalys G. Pritchard

USF Tampa Graduate Theses and Dissertations

This capstone project was part of a group project completed by five school and district administrators in Hillsborough County, Florida. The project began because of our passion for teachers who are able to establish a culture of care in their classrooms that support students academically but transform their learning through experiences that enable them to be more highly engaged and productive students, regardless of ethnicity, socioeconomic status, perceived academic abilities, and backgrounds.

My focus in this group project looked at social emotional learning, student behavior, and student achievement in high needs schools. Social Emotional Learning (SEL) and its related activities …

Assessing The Impacts Of Ghana’S Oil And Gas Industry On Ecosystem Services And Smallholder Livelihoods, Michael Acheampong

USF Tampa Graduate Theses and Dissertations

Ghana discovered oil and gas within its shores in commercial quantities in the year, 2007. This discovery was hailed as a potential turn around for the country’s economic destiny. While this optimism may have been well-founded, there has been a relative lack of appreciation of the potential adverse implications of the industry on traditional smallholder livelihoods, and the critical ecosystems that form their basis. In many countries, discovery of natural resources results in negative environmental and social outcomes, due to the loss of local livelihoods and environmental degradation. Deterioration of local livelihoods occurs through several biophysical pathways, both simple and …

Germania-Based Sol-Gel Coatings And Core-Shell Particles In Chromatographic Separations, Chengliang Jiang

USF Tampa Graduate Theses and Dissertations

Chapter one reviewed the development of sol-gel stationary phase for gas chromatograph (GC). Inorganic sol-gel precursor creates the substrate support for stationary phase bind to silica surface through the condensation of silanol groups, the rough surface with enhanced surface area enlarger the capacity of the sol-gel stationary phase, the porosity of sol-gel structure decreases the mass transferring coefficient, the term C in Van Deemter equation, which makes sol-gel stationary phase thicker coating up to 1 um but keeps the high resolution for gas chromatography. Chemical bound stationary phase significantly improve GC column with better thermal stability and solvent stability. Partial …

Using Ecosystem-Based Modeling To Describe An Oil Spill And Assess The Long-Term Effects, Lindsey N. Dornberger

USF Tampa Graduate Theses and Dissertations

The goal of the research conducted in this dissertation was to define and test methods to incorporate oil spill effects into an ecosystem-based assessment model. It was instigated by the Deepwater Horizon oil spill, an unprecedented oil spill in the United States for both depth and volume, with unknown implications for the health of the region. Using an ecosystem-based assessment model like Atlantis, with integrated oil spill dynamics, was the ideal candidate to predict long-term impacts such as decreased abundance or population recovery time. However no previous methodology existed for doing so in any ecosystem-based assessment model. Therefore, first I …

Validating The Accuracy Of Neatwork, A Rural Gravity Fed Water Distribution System Design Program, Using Field Data In The Comarca Ngöbe-Bugle, Panama, Maria Briones

USF Tampa Graduate Theses and Dissertations

Despite the sustainable development goals to increase access to improved water there are still 884 million people in the world without access to an improved water source (WHO, 2017). One method to improve access to water in rural, mountainous areas, is through construction of gravity fed water distribution systems. These systems should be designed based upon fundamental principles of hydraulics. One method of doing so in a time efficient manner with minimal engineering knowledge is to utilize a downloadable computer program such as Neatwork, which aids in design of rural, gravity fed water distribution systems and has been used by …

Academic Stress, Depression, And Social Support: A Comparison Of Chinese Students In International Baccalaureate Programs And Key Schools, Wenjun Chen

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine how academic stress affects Chinese high achieving secondary students’ mental health. Potential moderating effects of perceived social support and gender on the relationship between academic stress and depression were also examined. Current literature suggests Chinese high school students report greater academic stress and depression than their counterparts overseas (Sun, Dunne, Hou, & Xu, 2013), but it is unclear about the status of high achieving Chinese students as well as how social support works as a protective factor on this particular population. In order to fill this gap, the current study recruited a …

Application Of The Fusion Model For Cognitive Diagnostic Assessment With Non-Diagnostic Algebra-Geometry Readiness Test Data, Robert H. Fay

USF Tampa Graduate Theses and Dissertations

This study retrofitted a Diagnostic Classification Model (DCM) known as the Fusion model onto non-diagnostic test data from of the University of Chicago School Mathematics Project (UCSMP) Algebra and Geometry Readiness test post-test used with Transition Mathematics (Third Edition, Field-Trial Version). The test contained 24 multiple-choice middle school math items, and was originally given to 95 advanced 6th grade and 293 7th grade students. The use of these test answers for this study was an attempt to show that by using cognitive diagnostic analysis techniques on test items not constructed for that purpose, highly predictable multidimensional cognitive attribute profiles for …

A Participatory Action Research Using Photovoice To Explore Well-Being In Young Adults With Autism, Gary Yu Hin Lam

USF Tampa Graduate Theses and Dissertations

Young adults with autism transitioning from school to adulthood are commonly described as exhibiting poor outcomes. Although there has been research efforts measuring quality of life and life satisfaction in individuals with autism, these conceptualizations of well-being are still predominantly deficit-focused and based on normalizing ideals of the dominant culture. Only by incorporating individuals with autism’s perspectives and involving their meaningful participation in research can we better understand and promote well-being among individuals with autism. The present study aims to explore young adults with autism’s ideas about well-being. I conducted a Photovoice project using a participatory action research approach with …

Human Activity Recognition Based On Transfer Learning, Jinyong Pang

USF Tampa Graduate Theses and Dissertations

Human activity recognition (HAR) based on time series data is the problem of classifying various patterns. Its widely applications in health care owns huge commercial benefit. With the increasing spread of smart devices, people have strong desires of customizing services or product adaptive to their features. Deep learning models could handle HAR tasks with a satisfied result. However, training a deep learning model has to consume lots of time and computation resource. Consequently, developing a HAR system effectively becomes a challenging task. In this study, we develop a solid HAR system using Convolutional Neural Network based on transfer learning, which …