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Surface Functionalization And Analysis Thereof For An Ovarian Cancer Diagnostic Biosensor, Asad Ali Ahmad

USF Tampa Graduate Theses and Dissertations

Ovarian cancer is the fifth leading cause of cancer death among women in United States and has an alarming 1.4% (1 in 71) lifetime risk. The lack of overt symptoms and the absence of a reliable screening test to detect ovarian cancer result in over 70% of women being diagnosed after the disease has spread beyond the ovary resulting in a poor prognosis. A key characteristic of ovarian cancer is the ability of tumor cells to evade apoptosis, or programmed cell death contributing to the limitless replicative potential, which is a hallmark of all carcinogenesis. There is conclusive evidence that …

Development Of A Non-Invasive Electrode For Intradermal Electrically Mediated Dna Vaccination, Amy Lynn Donate

USF Tampa Graduate Theses and Dissertations

Current progress in the development of vaccines has decreased the incidence of fatal and non-fatal infections and increased longevity. However, new technologies need to be developed to combat an emerging generation of infectious diseases. DNA vaccination has been demonstrated to have great potential for use against a wide variety of diseases. Alone, this vaccine technology does not generate a significant immune response for vaccination, but combined with delivery by electroporation (EP), can enhance plasmid expression and immunity against the expressed antigen. Most EP systems, while effective, can be invasive and painful making them less desirable for use in vaccination. Our …

Discovery Of Novel Viruses From Animals, Plants, And Insect Vectors Using Viral Metagenomics, Terry Fei Fan Ng

USF Tampa Graduate Theses and Dissertations

Understanding emerging viruses is critical for disease monitoring and prediction; however, surveys of novel viruses are hindered by the lack of a universal assay for viruses. Viral metagenomics, consisting of viral particle purification and shotgun sequencing, is a powerful technique for discovering viruses in a wide variety of sample types. However, current protocols are not effective on tissue samples (e.g., lungs, livers and tumors), where they are hindered by the high amount of host nucleic acids which limits the percentage of sequences that originate from viruses. In this dissertation, a modified viral metagenomics protocol was developed and utilized to effectively …

Inactivation Of Ascaris Suum By Ammonia In Feces Simulating The Physical-Chemical Parameters Of The Solar Toilet Under Laboratory Conditions, Ligia Maria Cruz Espinoza

USF Tampa Graduate Theses and Dissertations

Access to sustainable sanitation systems is a determining factor in human health and economic development. However, more than a third of the world’s population lives without access to improved sanitation facilities. To meet the sanitation United Nations Millennium Development target, "halve, by 2015, the proportion of people without sustainable access to safe drinking water and basic sanitation", a wide range of non conventional sanitation technologies have been implemented in developing countries, including waterless systems. These systems function by diverting urine away from feces and collecting, storing, and dehydrating the fecal material in watertight dehydration vaults. From a public …

Melville’S Unknown Pathology: The Humoral Theory Of Disease And Low Grade Lead Poisoning In Bartleby The Scrivener, Gerard Bogin

USF Tampa Graduate Theses and Dissertations

Melville wrote Bartleby the Scrivener as a literary portrayal of the Humoral theory of disease. Virchow disproved that theory five years after the novella was published, suggesting Melville was humanizing an unknown pathology. A clinical assessment of the text reveals low-grade lead poisoning, which best explains the strange behavior, abnormal appearance, and premature death of the character Bartleby as depicted by the author. In conjunction with the textual substantiation, historical evidence indicates that at the time Melville wrote the work, one in ten people he encountered suffered from the effects of the same disease. Informed with the identity of Melville’s …

Small Plots, Big Hopes: Factors Associated With Participation In An Urban Garden Project In Lesotho, Charlotte Ann Noble

USF Tampa Graduate Theses and Dissertations

Studies of food insecurity have frequently focused on rural dwellers as vulnerable

populations. However, during the ‘global food crisis’ of 2007-2008, riots in more than

50 countries visibly demonstrated the vulnerability of urban populations to food

insecurity due to rapidly rising food prices. This study examines factors associated with

participation in an urban garden project (UGP), utilizing surveys (n=61) and in-depth

household interviews (n=37) to examine food security and dietary diversity of households

in urban Lesotho.

Households that participated in the garden project were more food insecure and

had lower dietary diversity than those that did not participate. However, it …

Migration, Education, And Health Policy: A Closer Look Into The Reasons Behind Poor Health Outcomes In Rural Ecuador, Lauren Harris

USF Tampa Graduate Theses and Dissertations

Despite an increase in the number of Ecuadorian medical professionals, health outcomes in rural areas of the country have steadily declined over the past decade. Using a political economic framework and data collected from interviews with Ecuadorian doctors, government officials, policy makers, and local rural populations, this thesis investigates how the interplay among medical migration, educational structures, and public health policy contributes to growing health disparities between urban and rural dwellers. Addressing each of these factors both individually and collectively, this thesis also outlines a series of policy recommendations that will allow the Ecuadorian healthcare system to better meet the …

Plasma Mediated Molecular Delivery, Richard J. Connolly

USF Tampa Graduate Theses and Dissertations

Non-viral delivery of plasmid DNA has traditionally relied upon physical forces applied directly to target tissues. These physical methods typically involve contact between an applicator and the target tissue and often cause transient patient discomfort. To overcome the contact-dependent limitations of such delivery methodologies, an atmospheric direct current plasma source was developed to deposit ionized gas molecules onto localized treatment sites. The deposition of charged species onto a treatment site can lead to the establishment of an electric field with strengths similar to those used for traditional electroporation. In vitro experiments proved that this technology could transiently permeabilize cell membranes …

Enhancing Virus Surveillance Through Metagenomics: Water Quality And Public Health Applications, Karyna Rosario-Cora

USF Tampa Graduate Theses and Dissertations

Monitoring viruses circulating in the human population and the environment is critical for protecting public and ecosystem health. The goal of this dissertation was to incorporate a viral metagenomic approach into virus surveillance efforts (both clinical and water quality control programs) to enhance traditional virus detection methods.

Clinical surveillance programs are designed to identify and monitor etiological agents that cause disease. However, the ability to identify viruses may be compromised when novel or unsuspected viruses are causing infection since traditional virus detection methods target specific known pathogens. Here we describe the successful application of viral metagenomics in a clinical setting …

Puerperal Fever In Britain: Failed Models Of Disease Causation, Jessica Wells

USF Tampa Graduate Theses and Dissertations

In eighteenth- and nineteenth-century Britain, a bacterial infection which we now

know to be caused primarily by a streptococcus, was killing women in childbirth at an

alarming rate. The disease, called puerperal, or childbed, fever, was being transmitted

primarily from doctor to patient by a doctor’s unwashed hands and filthy, contaminated

clothing and linens. Despite this evident and, in retrospect, obvious vector, the doctors of

this period never discovered how to prevent their patients from dying a gruesome and

painful death. Many physicians wrote extensive accounts of the illness but often ended

their works in despair, unable to find the …

Evaluation Of Oncology Nurses' Knowledge, Practice Behaviors, And Confidence Specific To Chemotherapy Induced Peripheral Neuropathy, Rebecca Denise Mcallister

USF Tampa Graduate Theses and Dissertations

Chemotherapy induced peripheral neuropathy (CIPN) remains one of the most serious and challenging symptoms oncology nurses encounter in caring for patients receiving neurotoxic chemotherapy. CIPN is under-addressed, under-reported, and symptoms are minimized by healthcare providers, which adversely affect patient quality of life, physical function, and emotional well-being. There is an absence of research examining nurses’ knowledge and practice behaviors related to CIPN. The purpose of this study was to explore oncology nurses knowledge, practice behaviors, confidence, and the relationship between education, experience, and knowledge specific to CIPN.

Data was collected at Oncology Nursing Society (ONS) Chapter meetings throughout central and …

The Use Of Proteomic Techniques To Study The Physiology And Virulence Of Staphylococcus Aureus, Frances Rivera

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus

is a bacterial pathogen that is believed to be the most common agent of human infectious disease, causing conditions ranging from common skin lesions to life-threatening illnesses. S. aureus has also shown a remarkable ability to develop resistance to antimicrobial treatment, making infections difficult to treat. In the post-genomic era, proteomic studies analyzing the protein complement of a genome in a particular organism at any given time, have gained real significance. This result is largely due to dynamic changes in protein expression profiles which can lead wide alterations in physiology and behavior. For proteomics, it is necessary to …

The Relationship Between Knowledge And Beliefs About Human Papillomavirus, Acceptance Of The Human Papillomavirus Vaccine, And Intentions To Practice Safer Sex Behaviors Among Female College Students, Theresa Scorcia-Wilson

USF Tampa Graduate Theses and Dissertations

Introduction. Sexually transmitted infections (STIs) continue to be highly prevalent among young women, and STIs continue to be a challenging health issue on college campuses. Studies have shown that the highest prevalence of human papillomavirus (HPV) is among young adult women, ages 20 to 24, including female college students. While the HPV vaccine has proven to be highly effective in preventing certain high-risk types of HPV, it is not effective in preventing all types of HPV or other STIs. Practicing other safer sex behaviors, in addition to condom use, also can help individuals protect themselves and their partners from …

Molecular Mechanism Of Agc Kinases In Human Malignant, Shaokun Shu

USF Tampa Graduate Theses and Dissertations

The maintenance of normal cell function and tissue homeostasis is dependent on the precise regulation of multiple signaling pathways that control cellular decisions to either proliferate, differentiate, arrest cell growth, or initiate programmed cell death (apoptosis). Cancer arises when clones of mutated cells escape this balance and proliferate inappropriately without compensatory apoptosis. Deregulated cell growth occurs as a result of perturbed signal transduction that modulates or alters cellular behavior or function to keep the critical balance between the rate of cell-cycle progression (cell division) and cell growth (cell mass) on one hand, and programmed cell death (apoptosis, autophagy) on the …

Utilizing Facebook Application For Disaster Relief: Social Network Analysis Of American Red Cross Cause Joiners, Jennie Wan Man Lai

USF Tampa Graduate Theses and Dissertations

With the exponential growth of Facebook users worldwide, this platform for social network online has become a powerful tool to connect individuals and share information with each other. This study explores the phenomenal trend of utilizing a Facebook application called Causes to help users organize into online communities for a specific cause and mobilize their resources for disaster relief during the Haiti earthquake disaster. Two separate samples of 100 joiners each from the American Red Cross (ARC) Cause on Facebook were randomly selected before and after the Haiti earthquake disaster to examine the differences of the composition (i.e., attributes) and …

Characterization Of Unidentified Viruses From Florida, Jessie L. Dyer

USF Tampa Graduate Theses and Dissertations

Public Health and clinical laboratories occasionally obtain viral isolates that cannot be typed by routine methods. Therefore, the sequence-independent, single primer amplification (SISPA) technique was adapted to rapidly identify and characterize viral isolates of unknown etiology. A panel of known (West Nile virus and St. Louis encephalitis virus) and unknown viral isolates (environmental samples collected in Florida) were used to develop and refine the SISPA technique. Selectivity for viral genomic sequences was obtained through enriching viral particles by centrifugation, removal of cellular debris by filtration and removal of host genomic material by benzonase application. The SISPA method successfully amplified the …

Malaria In Prehistoric Sardinia (Italy): An Examination Of Skeletal Remains From The Middle Bronze Age, Teddi J. Setzer

USF Tampa Graduate Theses and Dissertations

Sardinia was an island with a history of a malarious environment until eradication efforts were conducted from 1946 to 1950. While historic documents suggest the disease was introduced from North Africa around 500 BC, no study has been conducted to test for the presence of malaria in prehistoric native populations, such as the Nuragic people of the Bronze Age. However, it has been suggested that aspects of the Nuragic culture, for example the stone structures found throughout the island, are adaptations to a malarious environment. The purpose of this dissertation is to test the hypothesis that malaria was present in …

An Analysis Of The Influence Of Sampling Methods On Estimation Of Drug Use Prevalence And Patterns Among Arrestees In The United States: Implications For Research And Policy, Janine Kremling

USF Tampa Graduate Theses and Dissertations

Using data from the Drug Use Forecasting (DUF) and the Arrestee Drug Abuse Monitoring (ADAM) programs collected by the National Institute of Justice the question whether the drug estimates of DUF, using a non-probability sample, and the drug use estimates of ADAM, using a probability sample, yield substantially different results will be explored. The following main questions will be addressed using equivalence analysis: Are there substantial differences in the DUF and ADAM samples with regard to the drug use information obtained from arrestees at nine sites across the United States? The analysis suggests that the drug use information contained in …

Life In The Florida Everglades: Bioarchaeology Of The Miami One Site, Cristina Echazabal

USF Tampa Graduate Theses and Dissertations

The bioarchaeology of prehistoric south Florida has been an area of archaeological interest for the last century because of the interplay between ancient populations and the unique environment of the Everglades. The purpose of this study is to analyze the pathology, demography and mortuary practice of the ancient Southeast Florida aboriginal population at Miami One to assess the similarity of Miami One to other south Florida populations during the prehistoric period. The Miami One site (8DA11) is one of many related sites located along the shore of the Miami River. It was continuously occupied from the Late Archaic (ca.1000 B.C.) …

Toward An Applied Anthropology Of Gis: Spatial Analysis Of Adolescent Childbearing In Hillsborough And Pinellas Counties, Florida, Kathleen I. Maes

USF Tampa Graduate Theses and Dissertations

This work investigates births to white, African American and Hispanic adolescents in Hillsborough and Pinellas Counties, Florida, from 1992 to 1997 in two age groups - 13 to 17 year-olds and 18 to 19 year-olds - using spatial statistical techniques along with key informant interviews to provide insights into the utility of the research findings. The research developed a method for estimating the adolescent population in inter-census years, which was used to determine denominators for calculating teen birth rates. It also developed a composite deprivation index using socioeconomic indicators at the census block group level. The index provided context for …

Filtration Efficiency Of Surgical Masks, Erin Sanchez

USF Tampa Graduate Theses and Dissertations

Surgical masks are intended to be used to prevent transmission of disease from a health care worker to a patient. Often times, they are relied upon by health care workers for their own protection. In light of recent developments regarding preparation for health care worker response to global infectious diseases such as H1N1 Influenza, health care workers may experience a false sense of security when wearing surgical masks. The goal of this study was to evaluate the filtration efficiency of a double strap tie-on surgical mask. The manufacturer asserts a >95% efficiency with a 0.1 um challenge aerosol under FDA …

Active Surveillance And Incidence Rate Of Dengue Infection In A Cohort Of High Risk Population In Maracay, Venezuela., Carlos Espino

USF Tampa Graduate Theses and Dissertations

In the absence of an effective vaccine, vector control and surveillance of dengue fever (DF) and dengue hemorrhagic fever (DHF) are the most important strategies currently used to reduce the impact of these diseases in affected population. The objectives of this study were to estimate the incidence of symptomatic and asymptomatic dengue cases, the prevalence of antidengue antibodies, and to evaluate the laboratory and clinical aspects related to an active surveillance of dengue cases. In this study, active surveillance was incorporated as a part of the study design. At total of 3,255 people from four high risk neighborhoods were followed …

Factors Surrounding And Strategies To Reduce Recapping Used Needles By Nurses At A Venezuelan Public Hospital, Luis J. Galindez Araujo

USF Tampa Graduate Theses and Dissertations

Nurses as health care workers are at risk of biological agents such as bacteria, viruses and others. At health care settings exposure to bloodborne pathogens can cause infections through needlestick injuries. The objectives of this research were to determine factors surrounding recapping needles in hospital nurses and to implement an educational strategy to reduce the recapping practices.

It was a descriptive and exploratory approach where the PRECEDE component of the PRECEDE/PROCEDE Model was used as the framework to systematize and analyze the information obtained from the focus group sessions.

A total of 120 nurses participated from four different departments. The …

Hunger Of The Body, Hunger Of The Mind: The Experience Of Food Insecurity In Rural, Non-Peninsular Malaysia, Elizabeth Elliott Cooper

USF Tampa Graduate Theses and Dissertations

Supplementary feeding continues to be a widespread strategy for child health promotion though its efficacy remains contested. The long-standing, Malaysian national food assistance program for children - Program Pemulihan Kanak-Kanak Kekurangan Zat Makanan (PPKZM) - fits this pattern, receiving severe criticism for its limited impact on child nutritional status. Still, the program remains, producing a seeming paradox and prompting questions of how it fits into (1) the larger political context of national health policy and (2) more localized village and clinic environments. This research combines historical inquiry with the in-depth, ethnographic study of two predominantly Malay coastal villages in Malaysian …

Recombinant Aav Gene Therapy And Delivery, Nikisha Christine Carty

USF Tampa Graduate Theses and Dissertations

Alzheimer's disease (AD), first characterized in the early 20th century, is a common form of dementia which can occur as a result of genetic mutations in the genes encoding presenilin 1, presenilin 2, or amyloid precursor protein (APP). These genetic alterations can accelerate the pathological characteristics of AD, including the formation of extracellular neuritic plaques composed of amyloid beta peptides and the formation of intracellular neurofibrillary tangles consisting of hyperphosphorylated tau protein. Ultimately, AD results in gross neuron loss in the brain which is evidenced clinically as a progressive decline in mental capacity. A strong body of scientific evidence has …

Aggression And Its Consequences In Nursing: A More Complete Story By Adding Its Social Context, Liu-Qin Yang

USF Tampa Graduate Theses and Dissertations

Using a 471-case nursing sample, the current study examined the direct and indirect relationships between workplace aggression (including physical and psychological) against nurses and their health and safety consequences. Specifically, physical and psychological aggression nurses experienced were related to their job dissatisfaction, turnover intention, physical symptoms, injuries and exposure to contagious disease directly and/or indirectly through their emotional strain (irritation, anxiety, and depression). In addition, my findings demonstrated that stronger violence prevention climate (i.e., good prevention practices/response and low pressure for unsafe practices) was related to less frequent violence and psychological aggression incidents nurses experienced. Also, my results indicated significant …

Emergency Department Capacity Planning For A Pandemic Scenario: Nurse Allocation, Florentino Antonio Rico

USF Tampa Graduate Theses and Dissertations

The problem considered in this research is the efficient allocation of resources in an emergency department during a large flow of patient consequent to a pandemic influenza breakout. Predicting the impact of a Pandemic Influenza is very complex due to the many unknown variables that may play a role to how severe a pandemic can be. Scenario planning is considered in this research to forecast different potential outcomes and help decision makers better understand the role of uncertainties and become prepared to make important decisions. The goal is to first create a forecast model to estimate the patient demand during …

The Political Ecology Of Intestinal Parasites Among Nicaraguan Immigrants In Monteverde, Costa Rica, Jason D. Lind

USF Tampa Graduate Theses and Dissertations

Over the past 15 years Monteverde, Costa Rica has undergone rapid economic, social, political, and environmental change due to a flourishing ecotourism economy. While the effects of ecotourism development in Monteverde are many, two important consequences have been: 1) the immigration of Nicaraguan nationals to the area seeking low-skilled wage labor; and 2) compromised water resources management due to pollution and rapid population growth. The objective of this research is to investigate and identify the inter-relationships between ecotourism development in Monteverde and its affect on infectious diseases outcomes within the context of immigration and water resources management. Specifically, this dissertation …

Maternal Knowledge, Attitudes And Practices And Health Outcomes Of Their Preschool-Age Children In Urban And Rural Karnataka, India, Angela Lloyd

USF Tampa Graduate Theses and Dissertations

This cross-sectional, community-based study was designed to compare the health outcomes of 2 - 5 year-old children in different types of preschools. The Integrated Child Development Services (ICDS), run by the government of India, created a system of preschools, called anganwadis, to combat malnutrition, provide health education for mothers, and preschool for children 2 - 6 years old in 1975. Many children attend their local anganwadis, while others attend private schools, and others do not attend school at all. A pre-tested questionnaire was used to interview 125 urban and 130 rural mothers regarding their knowledge, attitudes, and practices about acute …

Functional Characterization Of Cytochrome B5 Reductase And Its Electron Acceptor Cytochrome B5 In Plasmodium Falciparum, Lucio Malvisi

USF Tampa Graduate Theses and Dissertations

Malaria is a disease of major public health importance, killing approximately one million people and causing about 250 million cases of fever annually. It mostly affects children under the age of five and pregnant women in many developing countries, making it a prominent issue in international health and maternal and child health. The most aggressive form of malaria is caused by the parasite Plasmodium falciparum which is responsible for 80% of infections and 90% of deaths from malaria, and is most prevalent in sub-Saharan Africa. Public Health interventions include the implementation of prevention programs, health education, and chemotherapy. The latter …