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Learning Connections Inventory© Validation Using A Physician Assistant Training Program, Roberta Worsham

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to validate the Learning Connections Inventory for use with the military Physician Assistant students at the Interservice Physician Assistant Program at Fort Sam Houston, TX. The 61 participants in this study completed both a demographic survey and the Learning Connections Inventory (LCI©). The results of the demographic questionnaire depicted the characteristics of the participants and the LCI© described the learning patterns of each participant.

Research Questions one, confirmation of the current LCI© model, and two, the instructor assessment and the current model agreement, were answered using confirmatory factor analysis (CFA) and construct validity. The …

Fighting Mass Diffusion Of Fake News On Social Media, Abdallah Musmar

USF Tampa Graduate Theses and Dissertations

Fake news has been considered one of the most challenging problems in the last few years. The effects of spreading fake news over social media platforms are widely observed across the globe as the depth and velocity of fake news reach far more than real news (Vosoughi et al., 2018). The plan for the following dissertation is to investigate the mass spread of fake news across social media and propose a framework to fight the spread of fake news by mixing preventive methods that could hinder the overall percentage of fake news sharing. We plan to create a study on …

Unraveling The Role Of Novel G5 Peptidase Family Proteins In Virulence And Cell Envelope Biogenesis Of Staphylococcus Aureus, Stephanie M. Marroquin

USF Tampa Graduate Theses and Dissertations

Virulence factors and the bacterial cell envelope are two important components in S. aureus pathogenesis and survival. More importantly, understanding the regulation of these cellular processes is crucial to further understanding and combating this successful pathogen. To date, numerous factors have been identified as playing a role in the regulation of Agr activity in S. aureus, including transcription factors, antisense RNAs, and host elements. Herein we investigate the product of SAUSA300_1984 (termed MroQ), a transmembrane G5 peptidase family protein, as a novel effector of this system. Using a USA300 mroQ mutant we observed a drastic reduction in proteolysis, hemolysis, and …

Engagement And Meaningfulness As Determinants Of Employee Retention: A Longitudinal Case Study, Calvin Williams

USF Tampa Graduate Theses and Dissertations

The lack of financial resources has long challenged government institutions to move employee wages toward the market rate average for hourly and salaried positions. This area of public employment, where motivation other than a pay increase is necessary due to constrained budgets, is not well studied or understood. The primary purpose of this longitudinal case study is to investigate factors that influence Administrative Service employees and increase their level of retention at the University of South Florida (USF), a state funded university. The objective of the case study was to better understand how enhanced psychological meaningfulness, and increased employee engagement …

Shortcut Nitrogen Removal In Photo-Sequencing Batch Reactor, Experiments, Dynamic Model And Full-Scale Design, Sahand Iman Shayan

USF Tampa Graduate Theses and Dissertations

Nitrogen is one of the essential elements for life; however, inefficient use of nitrogen creates major environmental problems. Nitrogen-rich streams from urban areas, farms, and wastewater treatment plants (WWTPs) lead to surface and groundwater contamination, resulting in eutrophication, hypoxia, and notable environmental, public health and economic damage. Ammonia is a form of nitrogen that can be created during industrial or biological nitrogen fixation or decomposition of biomass. Anaerobic digesters (ADs) in WWTP generate biogas from collected sludge from primary and secondary clarifiers to achieve energy recovery in the plant. However, sidestream effluent from dewatering AD sludge contains high concentrations of …

Effectiveness Of An Early Literacy Intervention For Increasing Teen Parents' Child-Directed Speech And Conversational Turns, Deborah H. Christie

USF Tampa Graduate Theses and Dissertations

Children’s language ability upon entry to kindergarten is a powerful predictor of reading achievement throughout elementary school; yet disparities in children’s language growth have been detected as early as 18 months of age. These disparities have been linked to the quantity and quality of speech provided to children as they are learning to talk. The current study employed a single-case multiple-baseline across participants experimental design to evaluate the effectiveness of an early literacy intervention to increase teen parents’ child-directed speech and conversational turns. The intervention was delivered one-on-one via videoconferencing by a teen parent peer coach. Participants included teen parents …

Constructing 'Child Safety': Policy, Practice, And Marginalized Families In Florida's Child Welfare System, Melissa Hope Johnson

USF Tampa Graduate Theses and Dissertations

‘Child safety’ has become a central concept of the modern child welfare system, an institution whose purpose is to protect children from abuse and neglect. What safety means and how it is best accomplished, however, are highly contested and characterized by definitional ambiguity, inconsistent bureaucratic interpretation, and operational variability. Situating this research within the anthropology of the state, the purpose of the current study is to develop a deeper understanding of the ways in which the state enacts power in matters of the family and childrearing through the child welfare system, casting a critical lens on the strategies used in …

Exercise Prescription Practices In University Counseling Centers: Testing The Information-Motivation-Behavioral Skills Model, A'Naja M. Newsome

USF Tampa Graduate Theses and Dissertations

The co-occurence of physical inactivity and poor mental health in the college student population can lead to chronic health issues that have negative short-term (e.g., academic success and weight gain) and long-term (e.g., obesity, serious mental illness, and premature mortality) impacts. Integrating exercise prescription into the mental health treatment plan of college students could enhance the holistic care model described by The American College Health Association (ACHA) and Healthy Campus task force. Understanding the knowledge, attitudes, and skills that mental health professionals (MHPs) hold regarding exercise prescription is important for policy formation and program development for college student health. The …

Examining The Effects Of Hydrology And Reclaimed Water Application On Nutrient Retention In Wetlaculture Mesocosms In Southwest Florida, Andrew Wilson

USF Tampa Graduate Theses and Dissertations

The substantial increase among agricultural and urban land use over the past century is widely regarded as the leading cause in a variety of negative environmental impacts, particularly regarding eutrophication of both salt and fresh surface waters. Usage of both synthetic and natural fertilizers on agricultural fields and residential lawns has increased significantly in order to achieve maximum crop yield and green lawns. The nitrogen and phosphorus in these fertilizers inevitably run off the landscape and into downstream surface waters. The geographic scale of this issue makes it a challenge to overcome, as targeting specific sources/polluters is nearly impossible. Landscape-scale …

Differential Privacy For Regression Modeling In Health: An Evaluation Of Algorithms, Joseph Ficek

USF Tampa Graduate Theses and Dissertations

Background: There is a need for rigorous and standardized methods of privacy protection for shared data in the health sciences. Differential privacy is one such method that has gained much popularity due to its versatility and robustness. This study evaluates differential privacy for explanatory regression modeling in the context of health research.

Methods: Surveyed and newly proposed algorithms were evaluated with respect to the accuracy (bias and RMSE) of coefficient estimates, the empirical coverage probability of confidence intervals, and the power and type I error rates of hypothesis tests. Evaluations took place in both simulated and real data from a …

An Assessment And Exploration Of Recent Methodological Advances In Safety Data Analysis, Suryaprasanna Kumar Balusu

USF Tampa Graduate Theses and Dissertations

The injury severity and frequency of traffic crashes can be further reduced by implementing appropriate traffic safety measures. Several advanced methodologies have been developed in the recent decades to more accurately identify the factors affecting road safety. Such methodologies have helped policy makers formulate appropriate safety measures directed toward increasing seatbelt and helmet use, reducing speed limits in school zones, implementing adolescent driver programs, and so on. However, each methodological approach has its own strengths and weakness and could lead to different inferences. This dissertation assesses and explores a number of recently developed methodologies and their application to traffic safety …

Understanding Player And Clinician Perspectives On The Impact Of Problematic Gaming Behaviors In The United States, Amanda N. Weston

USF Tampa Graduate Theses and Dissertations

Due to the evolution of technology and increased use of video games, the need to understand how gaming affects individuals is imperative to assisting with those who struggle with problematic gaming behaviors. Video gaming has shown to have significant benefits including an increase in cognitive abilities, a way to relieve stress, and mood enhancement. However, problematic gaming behaviors (PGB) (i.e., uncontrollable gaming behaviors that impair functioning) may cause significant short and long-term problems that impact gamers throughout the lifespan if left untreated. There are critical gaps in the literature regarding how gaming impacts player functioning and how clinicians are managing …

Riverine And Estuarine Co2-System Studies On The West Coast Of Florida, Christopher S. Moore

USF Tampa Graduate Theses and Dissertations

Coastal and estuarine acidification impacts ecosystem health and economic resources and has both natural and anthropogenic components (Cai et al., 2021). Riverine input is one of several important factors that can influence acidification in coastal ecosystems. Rivers disgorging into coastal environments can create strong gradients, both spatial and temporal, that make accurate CO2-system characterization challenging. The work described in this thesis provides a baseline CO2-system study of four major rivers that flow into Tampa bay with an emphasis on seasonal change. As a second objective, this thesis examines the effects of HgII additions on CO2-system measurements in organic-rich estuarine waters. …

Charter School Management: Mo Interaction With Educational Inputs And Outcomes, Joseph C. Simmons

USF Tampa Graduate Theses and Dissertations

This study seeks to understand if the utilization of a management company has any interaction with the socioeconomic status (SES) of students served, the school’s academic performance, the percentage English Language Learners served, the percentage of highly qualified teachers per school site, the amount of student attrition/mobility, or the amount of disciplinary events. Ultimately this study seeks to determine the efficacy and utility of management organization utilization by studying inputs and outputs of Florida charter schools and disaggregating them based on utilization of Charter Management Organizations, Education Management Organizations, or their decision not to utilize a Management Organization. This study …

Narratives Of Success: How Honors College Newcomers Frame The Entrance To College, Cayla Lanier

USF Tampa Graduate Theses and Dissertations

Starting college marks an important period of transition for young people, as they manage multiple changes at once and begin to establish identities independent from their parents. The first year college student experience has been the focus of a great deal of academic research, as scholars and practitioners seek to discover the best way to support students and ensure they remain successfully enrolled at the university. However, very little of this research attends to the specific experiences of Honors College students. Further, a focus on the communicative process of transitioning, or organizational socialization, may add to what is currently known. …

Machine Learning For Species Habitat Analysis, Abigail Lavallin

USF Tampa Graduate Theses and Dissertations

Management and conservation initiatives will always be controlled by finite resources, whether financialor temporal. Understanding a species’ spatial ecology, and how its requirements vary across habitats and locations is key to a successful species management plan. During recent decades, it has been noted how many species populations have declined, despite conservation practices working to increase their numbers. The most prevalent impacts affecting fauna populations have come from anthropogenic change in the form of habitat loss and destruction, along with fragmentation, and global climate change. There is a clear need for management practices to now operate on an entire landscape instead …

Resilience And Health Outcomes Of Sexual Minority Middle-Aged And Older Adults, Christi L. Nelson

USF Tampa Graduate Theses and Dissertations

While research on lesbian, gay, and bisexual (hereafter referred to as sexual minority) middle-aged and older adults has increased over the past decade, there is still a critical need for more research on the health and resilience in this growing subpopulation. Research has provided evidence that sexual minority adults have an increased risk of negative health outcomes when compared to heterosexual adults. Research has also demonstrated possible resilience in sexual minority middle-aged and older adults; however, few studies have measured resilience in middle-aged and older adults. Gaining a better understanding of resilience in sexual minority adults may help identify modifiable …

Using Developmental Evaluation For Clinical Faculty Development In A New Academic Consortium, Candice Kunkle

USF Tampa Graduate Theses and Dissertations

This study aims to support the development of the series, particularly this evaluation study addresses the faculty development program within a newly formed academic partnership between teaching and community physicians. The evaluation addresses the opportunities, assets, and strengthens in the programming while constructing it for the intended users. While this study supports the evolution of the new program, it jointly enhances the collective’s efforts and innovative initiatives within a new academic partnership among leaders and clinical faculty through its ongoing development. The learning and actions derived from the assessments were guided by analysis from needs assessments and interviews as part …

Internship And Job Acquisition Experiences Of Veterans In A Graduate Cybersecurity Degree Program, Stephen P. Gary

USF Tampa Graduate Theses and Dissertations

My purpose in this qualitative inquiry was to explore how student veterans experience cybersecurity internships and job acquisitions. I chose Schlossberg’s individual transition theory viewed through a constructivist lens to support the inquiry. Five participants, who completed graduate cybersecurity internships and acquired cybersecurity positions, provided responses. I employed semi-structured interviews to collect data from the participants. I engaged reflexive thematic analysis to guide my data analysis. Based on my analysis, I generated the following discoveries applicable to the inquiry: (a) opportunities for developing practical experience are regarded as essential for successful participation in an internship; (b) a flexible internship program …

Learning, Planning For, And Reflecting On Teaching Mathematics For Social Justice: A Case Study Investigation Of A High School Mathematics Methods Course, Queshonda J. Kudaisi

USF Tampa Graduate Theses and Dissertations

While much is known about learning to teach for social justice in other content areas, less is known about how mathematics education students learn to teach mathematics for social justice, specifically at the high school level. Hence, this mixed-methods convergent case study addresses this gap in the research literature by examining and describing the experiences of mathematics education students learning to teach mathematics for social justice within the context of a high school mathematics methods course at a large research university in the southeastern United States. The data sources accessed for this study include a survey measuring beliefs about teaching …

Microbial Dark Matter: Culturing The Uncultured In Search Of Novel Chemotaxonomy, Sarah J. Kennedy

USF Tampa Graduate Theses and Dissertations

Although <1% of environmental isolates can be cultured, insights into the culturable fraction refine future techniques to unlock the cryptic biodiversity. This cryptic biodiversity is omnipresent within culture-independent surveys of environmental bacterial populations. Subsequently, these elusive organisms are termed “microbial dark matter” due to the intrigue surrounding their potential taxonomic and biosynthetic impacts. To this end of investigating bacterial-specific dark matter, we employed high-throughput culturing techniques to examine Gulf of Mexico sediments and sponges for chemotaxonomically important bacteria. Through stepwise implementation of permissive and selective recovery techniques, we succeeded in recovering over 150 unique bacterial isolates. We observed several isolates that pose as chemotaxonomically interesting, including several strains that belong to critical bioremediation taxa. In addition to these biotechnological-amenable isolates, we have also succeeded in culturing taxa historically attributed with antibacterial discoveries. One such isolate was a previously sponge-associated organism belonging to the rarely cultured Verrucosispora genus. Through extensive comparative genomics, we identified it as a novel species and subsequently named it Verrucosispora sioxanthis sp. nov. with the published genome serving as a resource for continued microbial dark matter investigations. Following continued passaging of the organism, we observed improved growth, biomass, and liquid growth dispersal within each increasingly sub-passaged generation of the isolate. As such, the laboratory attenuation, or domestication, of the organism warranted in-depth whole genomic analyses to generate insight into the increased laboratory sustainability. Through phenotypically-guided bioinformatics, we determined that the domestication was mediated through genome-wide selective pseudo- or non-pseudogenization of genes. As the previously sponge-associated organism evolved with the selection pressures of the static laboratory, we demonstrate that isolate effectively underwent genomic atrophy to resolve into a largely stable, sustainable laboratory-domesticated strain. Collectively, our chemotaxonomic discovery pipeline expands on the culturable bacterial biodiversity and ultimately provides a previously uncultured model organism for initial forays into resolving the microbial dark matter knowledge gap.

Online Perceptions Of Panamanian Prisons And Incarcerated Persons: An Analysis Of Youtube User Comments, Mahaleth J. Sotelo

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to explore and understand the frameworks in which prisons and incarcerated persons are discussed amongst commenters under YouTube videos displaying media on Panamanian prisons. The study incorporates a mixed methods approach by conducting a general content analysis of YouTuber comments to address themes within the discussion. Additionally, these themes were quantified and modeled using predictive variables collected such as number of comment likes, number of comment dislikes, and number of comment replies, alias type (screen name or name-like), presence of profile picture, and profile picture type. The themes found were 1) punitive, 2) justifying …

Sanitation Marketing In Uganda: The Impact Of Gender On Participation And Outcomes, Elizabeth Vicario

USF Tampa Graduate Theses and Dissertations

Uganda aims to reach universal access to basic sanitation under Sustainable Development Goal #6, but achieving this target by 2030 would require doubling the annual rate of progress. Women are disproportionately affected by poor sanitation due to their health needs and risk of facing violence, but they have historically been excluded from sanitation planning. Low household demand and poor service delivery have stalled progress in Uganda. Market-based sanitation (MBS) is a novel method of developing a sanitation market through stimulating demand and supporting a competitive atmosphere for product and service providers. This research studies the sanitation marketing activities implemented by …

Mobile Response Teams And The Youth Emergency Behavioral Health System, Paige J. Alitz

USF Tampa Graduate Theses and Dissertations

Introduction: This dissertation includes three separate manuscripts that coalesce under the shared topic of mobile crisis response and the emergency behavioral health system. Method: The first manuscript includes a synthesis of the research on mobile crisis response from the 1960s to present day to assess whether mobile crisis services can improve mental health care access in the U.S. for youth and adults. The second manuscript includes bivariate and multivariate analyses of MRT participant data to characterize participants who receive an involuntary psychiatric evaluation versus those who do not, and to assess factors associated with involuntary psychiatric evaluation or referral to …

Application Of Gold Nanoshells For Photothermal Therapy In Lung Cancer Cells, Yva K. Luc

USF Tampa Graduate Theses and Dissertations

Lung cancer is the leading cause of cancer deaths in the world. For the year 2020, it was estimated 1.8 million deaths were due to lung cancer alone, making it more lethal than breast cancer and prostate cancer combined. For the year 2021, it is estimated 235,760 new cases of lung cancer and 50% will lead to death. The primary cause of lung cancer is due to cigarette smoking, which accounts for 80% of all lung cancer deaths. Lung cancer screening relies on only different methods, biopsy, bronchoscopy, ultrasound, chest x-ray film, and sputum cytology. These techniques are used to …

Investigation Of An Alternative Protocol For The Production Of Sars-Cov-2 Antigenic Proteins, Nichole Ninaltowski

USF Tampa Graduate Theses and Dissertations

With the COVID-19 pandemic showing no signs of slowing down, large-scale antigenic protein production is still needed for surveillance using serologic assays. From screening to vaccines to biotherapeutics, being able to produce the proteins for these assays is essential; however, the current gold standard method for producing SARS-CoV-2 spike proteins is prohibitively expensive for most research groups.

Alternative methods of transfecting mammalian cells to produce recombinant proteins that are relatively inexpensive have been used for years. Unlike the expensive, commercially available lipid-based methods, other established methods such as polyethyleneimine (PEI), are considerably easier, and cheaper to meet the needs of …

Community Assessment Of Water Perceptions And Household Point-Of-Use Treatment Methods In Madagascar, Isabella Rose Silverman

USF Tampa Graduate Theses and Dissertations

One in four people around the world do not have access to safe drinking water, which means that two billion people globally lack access. In high-income countries, 0.02% of yearly deaths are attributed to unsafe water sources; however, an alarming 10.6% of deaths occur in Madagascar, for the same reason.

To achieve Sustainable Development Goal 6: Ensure access to water and sanitation for all, a collaboration between public health and engineering is critical. Within the public health sector, social marketing is commonly utilized to address a problem by using commercial marketing principles to change behavior for the good of society. …

Laboratory Examination Of Lead Weights Harvested From Pitcher Pumps In Eastern Madagascar, Madelyn Wilson

USF Tampa Graduate Theses and Dissertations

Previous sampling campaigns in Tamatave, Madagascar, conducted by the University of South Florida (USF) have identified lead contamination in drinking water sourced from self-supply pitcher pumps. The pitcher pump piston and foot valves were identified as the primary source of lead contamination since they are often manufactured from recycled lead acid batteries. The previous field sampling technique consisted of collecting an aliquot of a 10-15-liter composite sample from the pitcher pump for dissolved lead analysis; however, this technique (1) neglects the contribution of particulate lead, (2) does not properly account for the effects of water stagnation inside the pitcher pump …

Online And Adjusted Human Activities Recognition With Statistical Learning, Yanjia Zhang

USF Tampa Graduate Theses and Dissertations

Wearable human activity recognition (HAR) is a widely application system for our daily life. It hasbeen built in many devices, such as smartphone, smartwatch, activity tracker, and health monitor. Many researchers try to develop a system which requires less memory space and power, but has fast and accurate classification results. Moreover, the objective of adjusting the classifier by the system self is also a study direction. In the present study, we introduced the machine learning methods to both smartphone data and smartwatch data and an adjusted model with the continuous generating data. Further, we also proposed a new HAR system …

A Functional Characterization Of The Omega (Ω) Subunit Of Rna Polymerase In Staphylococcus Aureus, Shrushti B. Patil

USF Tampa Graduate Theses and Dissertations

In bacteria, RNA polymerase (RNAP) is a well-characterized and highly conserved multi-subunit enzyme complex responsible for transcription of DNA into RNA. The ω subunit (rpoZ/RpoZ), one of the smaller accessory subunits of RNAP, is often overlooked and under-studied, however, particularly in Gram-positive species. We have previously shown that deleting ω impacts the integrity of RNA polymerase in S. aureus, especially the β' subunit, and alters preference of the core enzyme for sigma factors, skewing heavily towards σB instead of the housekeeping σ factor. Consequently, this causes deregulation of myriad transcriptional processes, strongly rewiring gene expression circuits, and ultimately impairing the …