Digital Commons Network^™

Effect Of Dual Inhibition Of Atypical Pkc Signaling And Pi3k/Akt Signaling On Cell Viability And Apoptosis In Clear Cell Renal Cell Carcinoma (Ccrcc)., Khandker M. Khalid

USF Tampa Graduate Theses and Dissertations

Renal Cell Carcinoma (RCC) is the most common type of kidney cancer (85%); 75% of the cases involve conventional clear cell RCC (ccRCC). The choice of treatments recommended for clear cell carcinoma and non-clear cell carcinoma include partial nephrectomy (Stage I), radical nephrectomy (Stages II and III), and chemotherapy (stage IV). Phosphatidylinositol-3-kinase (PI3K) inhibitors can be a viable candidate in the chemotherapy of ccRCC because the PI3K/Akt pathway is frequently activated in RCC. Hence, PI3K inhibitors like Alpelisib (BYL719) can be chosen as a treatment. In addition, aPKCs (PKC-ί and PKC ζ) are overexpressed in most cancer cells, and they …

A Study Of Relationship Between Exertional Heat Illness And Heat Stress Levels, Rolando J. Fargas-Martinez

USF Tampa Graduate Theses and Dissertations

Introduction: Heat stress is clearly associated exertional heat illnesses (EHI) in workers in a number of studies. With expected increase in extreme thermal changes, an increase understanding the effects of heat stress conditions is necessary. The purpose of this study was to compare heat stress levels in a manufacturing plant to determine whether a relationship exists between heat stress level relative to the occupational exposure limit and exertional heat illness incidents.Methods: OSHA recordable heat illness cases (n=35) were collected over a three year period. Over the same period, hourly ambient Heat Index data was collected. For this manufacturing plant, heat …

Socio-Emotional Effects Of Rejection: An Experience-Sampling Examination, Gabriella Silva

USF Tampa Graduate Theses and Dissertations

Rejection is a detrimental experience that directly threatens people’s fundamental need for social acceptance and connection. Rejection is particularly distressing for people with elevated social anxiety, as it is their core fear. Yet, very few studies have examined the impact of daily rejection in the context of social anxiety. The present research examined the socioemotional effects of daily feelings of rejection in the context of social anxiety across two experience-sampling studies. Study 1 was a 2-week daily diary study with community adults and Study 2 was a 4-week ecological momentary assessment (EMA) study with a clinical sample of adults with …

Evaluation Of Two Methods To Estimate Wet Bulb Globe Temperature From Heat Index, Stephi Pofanl

USF Tampa Graduate Theses and Dissertations

The Wet bulb globe temperature (WBGT) is a common method to evaluate environmental factors that contribute to occupational heat stress. The Heat Index (HI) is readily available as an alternative to exposure methods. They both assess the most important contributors that are air temperature and humidity. WBGT also includes radiant heat and air motion. To account for radiant heat and/or air motion, some investigators have suggested adjustments to HI. The purpose of this study is to explore two methods to estimate the usefulness of WBGT from HI.

WBGT data were collected in a variety of locations that included direct sun …

Academic Procrastination Among Chinese International Undergraduate Students In The United States, Yunlu Xiang

USF Tampa Graduate Theses and Dissertations

This research aimed to explore academic procrastination among Chinese internationalstudents, using NVivo 12 to analyze data gathered through interviews with six participants. The study identified both internal and external factors contributing to academic procrastination, with social networking and software being the most significant external factors and disinterest and avoidance being the most significant internal factors. The participants did not identify the COVID-19 pandemic as a significant factor in academic procrastination. The study analyzed gender differences in academic procrastination among Chinese students. The pandemic had different effects on men and women, with men downplaying the concept of time and women experiencing …

Improving The Subjective Well-Being Of Autistic Youth Utilizing A Positive Psychology Intervention, Nicolette Bauermeister

USF Tampa Graduate Theses and Dissertations

This study utilized a single-case multiple-baseline design to analyze the effects of a ten-week multi-component positive psychology intervention, the Well-Being Promotion Program, on the subjective well-being of Autistic youth. This thesis addressed a gap in the literature regarding the effectiveness of positive psychology interventions when administered to Autistic middle schoolers. Three Autistic middle schoolers participated in this single-case design study. Dynamic decision-making was used to stagger intervention implementation across the three participants. Life satisfaction and positive/negative affect data were collected via a Qualtrics survey that was administered twice per week. Through visual analysis and Baseline Corrected Tau calculations, it was …

Development Of A Ddpcr Multiplex To Measure The Immune Response To Borrelia Burgdorferi., Kailey Marie Mccain

USF Tampa Graduate Theses and Dissertations

Zoonotic diseases account for a significant proportion of emerging infectious diseases (EIDs), with their dynamics being heavily influenced by the environment. While the effects of the environment on population-level disease dynamics have been extensively studied, there is limited research investigating the impact of the environment on individual-level disease susceptibility and transmission. This study aims to fill this gap by creating a tool to examine the variation in immune response to Borrelia burgdorferi, the causative agent of Lyme Disease, in two competent host species, Peromyscus leucopus and Peromyscus maniculatus. Two triplex droplet digital polymerase chain reaction (ddPCR) assays were developed to …

Metachronal Locomotion: Swimming, Scaling, And Schooling, Kuvvat Garayev

USF Tampa Graduate Theses and Dissertations

This dissertation deals with one type of underwater locomotion called metachronal swimming in which the organism sequentially beats its multiple appendages allowing phase lag between adjacent neighbors. Metachronally swimming species are widespread and include copepods, shrimp, ctenophores, and tomopterid worms to name few. First, using the high-speed recording and planar particle image velocimetry (PIV) measurement, I report on kinematics of fast metachronal swimmer and constructive vortex interactions among its appendages and discuss its implications for improved performance regarding the swimming. Second, I show how hydrodynamic performance of all metachronal swimmers (paramecia, copepods, tomopterid worms, krill etc.) can be scaled by …

Threats To School Safety: Examining Levels Of Community Violence And Its Relation To School-Related Threats, Dorie Ross

USF Tampa Graduate Theses and Dissertations

Despite significant media attention provided to school-based acts of violence, these tragic incidents are relatively rare across school environments, leading to increasing challenges in identifying students who may pose a threat. Previous approaches to school discipline, including policies such as zero tolerance, resulted in significant racial disparities among students who received disciplinary consequences inconsistent with the severity of their behaviors or threats. Alternatively, a relatively recent approach that emerged following a series of school shootings in the 1990s was school threat assessment, which focuses on prevention rather than prediction. While it is important to determine students who may pose a …

Preliminary Characterization And Functional Validation Of Pre-Erythrocytic Vaccine Candidates For The Malaria Parasite Plasmodium Vivax, Justin Lee Nicholas Iii

USF Tampa Graduate Theses and Dissertations

RTS,S/AS01, vaccine targeting malaria caused by Plasmodium falciparum, used in moderate to high malaria transmission, provides hope for pre-erythrocytic (PE) subunit malaria vaccine development. Despite this vaccine advance, efforts to develop an efficacious vaccine against the neglected parasite Plasmodium vivax have lagged. In addition, there exists a gap in our understanding of PE antigens that can induce broadly-neutralizing antibodies. In this dissertation, we provide the preliminary characterization of vaccine candidates targeting the PE of P. vivax. Through transcriptomic profiling data and a target product profile, we identified critical P. vivax antigens (SSP3, SPECT-1, and M2-MAEBL) that can potentially be targets …

An Analysis Of State Sex Education Policies To Explore The Inclusiveness Of School Based Sex Education, Amarah Scott

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to analyze state policies related to sex education proposed between 2018 and 2022 to determine the extent to which sex education needs of LGBTQ youth were being addressed. Previous research has shown that, on average, both abstinence-only and comprehensive sex education curricula do not include information that is relevant to LGBTQ youth. Much of the information included in sex education curricula such as pregnancy prevention and the benefits and disadvantages of condoms are most relevant to youth who identify as heterosexual. Not receiving relevant and accurate sex education puts LGBTQ youth at risk for …

Insect Classification And Explainability From Image Data Via Deep Learning Techniques, Tanvir Hossain Bhuiyan

USF Tampa Graduate Theses and Dissertations

Since the dawn of the Industrial Revolution, humanity has always tried to make labor more efficient and automated, and this trend is only continuing in the modern digital age. With the advent of artificial intelligence (AI) techniques in the latter part of the 20th century, the speed and scale with which AI has been leveraged to automate tasks defy human imagination. Many people deeply entrenched in the technology field are genuinely intrigued and concerned about how AI may change many of the ways in which humans have been living for millennia. Only time will provide the answers. This dissertation is …

Reading Suffering Between The Lines: Trauma And Witnessing In Old And Middle English Literature, Anca Olguta Giorgiana Garcia

USF Tampa Graduate Theses and Dissertations

Both literary trauma theorists and medieval literature scholars generally avoid analyzing Old and Middle English literature through the lens of trauma studies. There are multiple reasons for this reluctance, and perhaps the most elementary one is the misconception that medieval society was numb to pain because they experienced it more often than people today do. Another reason is the fact that trauma in general is seen as a product of the Industrial Revolution and of the interactions between humans and machines. Its terminology and diagnostic methods have only developed in the last two centuries, so applying modern concepts on medieval …

Initial Understanding Of Public Stigma Against Childhood Cancer Survivors, Nele Loecher

USF Tampa Graduate Theses and Dissertations

As childhood cancer survival rates climb to new records, it is increasingly important to focus on improving the psychological wellbeing of survivors living years beyond their diagnosis. One potential contributor to psychological distress for childhood cancer survivors (CCSs) is stigma. The fear of stigma can lead to CCSs concealing their identity as a CCS, which in turn can strain workplace and intimate relationships, creating a strain on CCSs’ social and work life. Additionally, the internalization of negative stereotypes or rejection of falsely positive stereotypes around cancer can exacerbate psychological distress. To establish what stereotypes and stigma CCS might be contending …

Variability Of Air Sampling Results Using Air-O-Cell Cassettes, Christina M. Haworth

USF Tampa Graduate Theses and Dissertations

No current federal or state standards for mold exposure exist, partly due to the lack of accurate methods for assessing fungal exposure. Exposure to airborne fungal spores can result in several symptoms such as allergic rhinitis, coughing, and fatigue. Non-industrial occupational settings are common and fungal exposures may occur in these environments. The purpose of this study is to evaluate the meaningfulness of fungal spore trap results from samples collected in both a corporate office and a university classroom. Air-O-Cell cassettes with a Zefon Bio-Pump were used to collect one outdoor and three indoor samples at each location on three …

Supramolecular Architectures Generated By Self-Assembly Of Guanosine And Isoguanosine Derivatives, Mengjia Liu

USF Tampa Graduate Theses and Dissertations

This dissertation is primarily divided into three parts: 1) Anion, solvent-mediated, tunable self-assemblies of isoguanosine (isoG). 2) Post-assembly modification of the non-covalent isoG-pentaplex for Cesium extraction. 3) Supramolecular asymmetric amine catalysis.

The structural conformational design of the isoG decamer was employed for systematic investigations involving various substituted groups at the C8 position of purine and ribose. A series of isoG analogs with C8-phenyl substitutions were synthesized and utilized for Cesium coordination. The proximity between purine and ribose structures limited pentaplex formation for C8-phenyl substituted isoG derivatives. Based on this observation, a deoxy isoG derivative with a modified ribose was employed …

Invisible Families, Clear Consequences: Work-Family Integration Among Employees In Same Gender Presenting Romantic Relationships, Joseph Regina

USF Tampa Graduate Theses and Dissertations

Recent qualitative studies have provided evidence LGBTQ+ individuals experience unique challenges related to integrating one’s romantic partner into their work life. Informed by signaling theory, the role of work-romantic partner integration supplies (WRPIS) as a variable of interest was tested as an outcome of formal and informal signals of inclusion and as a predictor of job satisfaction, romantic relationship satisfaction, and emotional exhaustion. Hypotheses were tested using a three-timepoint survey with a sample of 138 full-time employed individuals who were currently involved in a committed same-gender presenting romantic relationship. Results provide support informal signals of inclusion relate to WRPIS and …

The Influence Of Obesity And Lifestyle Factors On Cognition In Middle-Aged And Older Adults, Andrew J. Fiscella

USF Tampa Graduate Theses and Dissertations

Obesity has been linked to conditions including cardiovascular disease, cancer, and diabetes. While the relationship of these conditions with obesity is well-established, the relationship between obesity and cognitive function is murkier.

Study 1 examined the association between being overweight or obese and performance on tests of executive function and episodic memory. The possibility that overweight/obesity affects cognition differently in middle vs. older age was also examined. Data from 6,012 participants in the 2018 wave of the Health and Retirement Study (HRS) were used. Body mass index (BMI) and waist circumference were used as overweight/obesity measures. Neither BMI (p = .809) …

“We Need To Have A Place To Vent And Get Our Frustrations Out”: Addressing The Needs Of Mothering Students In Higher Education Using A Positive Deviance Framework, Melissa León

USF Tampa Graduate Theses and Dissertations

This study examined the experiences of mothering students at four different colleges using a positive deviance (PD) framework. PD is an approach that seeks to identify positive behavioral patterns that help members of a community overcome structural barriers (Gross, et al. 2017). The Positive Deviance Framework was applied to investigate how some mothering students are successful in college and how their experiences could potentially help new or struggling mothering students. Eleven mothering students were interviewed to determine what interventions could assist mothering students who lack representation in the traditional college environment, a situation that often leads to feelings of isolation. …

Protein Folding Kinetics Analysis Using Fluorescence Spectroscopy, Dhanya Dhananjayan

USF Tampa Graduate Theses and Dissertations

The conformational changes that occur during the folding of a protein is an intensely researched area because of the impact that it has on human health and cellular functions. Protein stability is crucial in the context of protein misfolding and aggregation due to its implications on misfolding diseases such as amyloid fibril degenerative diseases (Alzheimer’s, Parkinson’s). By examining the kinetics of protein folding, we can gain valuable information about the folding mechanism and help us identify potential targets for many of the protein misfolding diseases.

In this study, we present the folding and unfolding kinetics of TEM-1 β-lactamase from Escherichia …

A Novel Role For Enos In Regulating Lymphatic Valve Development During Embryogenesis, Drishya Iyer

USF Tampa Graduate Theses and Dissertations

Lymphedema is a disease that occurs when lymph flow is impaired, resulting in tissue swelling, fibrosis, chronic inflammation and recurrent secondary infections. Lymphatic valves play a critical role in maintaining unidirectional lymph flow and evidence for valve defects have been reported in lymphedema patients. The lack of drugs that can correct lymphatic valve defects warrants a better understanding of the molecular regulators of lymphatic valve development and maintenance. Lymphatic valves first develop during embryogenesis in response to mechanotransduction signaling pathways triggered by oscillatory lymph flow. Since eNOS (gene name: Nos3) is a well characterized mechanotransduction signaling molecule in blood vessels, …

Contribution Of Contextual Factors And Neuropathology To Dementia, Monica E. Nelson

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that has extensive biological heterogeneity. It is not clear the extent to which this heterogeneity may be detected in participants without dementia, how it relates to incident AD dementia, and whether contextual factors may change how neuropathology relates to incident AD dementia. Therefore, this dissertation was completed using data from the Alzheimer’s Disease Neuroimaging Initiative (ADNI; n = 1,703) and the Czech Brain Aging Study (CBAS; n = 385) to address the following aims: to assess biological heterogeneity in participants without dementia, to relate this heterogeneity to incident AD dementia, and to …

Evaluating A Behavioral Intervention For Performance Blocks In Gymnastics, Cara L. Shapiro

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to evaluate a gradual exposure and thought training treatment package to increase gymnasts’ successful completion of a performance block skill. Following baseline, the researchers implemented both a thought training procedure to alter participants’ negative thoughts into positive thoughts, and a gradual exposure procedure which included gymnastics drills that were small approximations to the performance block skill. A 2-point rating scale was used to indicate successful completion of the skills: 0= refusal, 1= the participant initiated the skill but failed to complete it successfully, 2= the participant initiated the skill and completed it successfully. Rating …

Exploring The Risk Narratives Of Transgender People Engaged In Do-It-Yourself Hormone Replacement Therapy, Brianna Rae Cusanno

USF Tampa Graduate Theses and Dissertations

This dissertation analyzed the risk narratives of transgender people engaged in do-it- yourself hormone replacement therapy. Transgender (trans) people, those whose gender identities and/or expressions defy normative constructions of gender, experience a variety of health inequities as a consequence of systemic oppression within U.S. society. Many of the health inequities observed in trans people can be traced to structural determinants, such as discrimination at home, work, and school that results in housing, food, and social instability. Yet, medical and public health researchers have often attributed trans health inequities to “high risk” behaviors in which trans people engage.

One such behavior …

An Evaluation Of Measurement Invariance Of Dsm-5 Borderline Personality Disorder Criteria Across Heterosexual, Lesbian, Gay, And Bisexual Adults, E. Elisa Carsten

USF Tampa Graduate Theses and Dissertations

There is a robust, yet poorly understood relationship between non-heterosexual orientation and borderline personality disorder (BPD), with lesbian, gay, and bisexual individuals evidencing greater BPD symptoms compared to heterosexual individuals. Recent evidence suggests possible bias in BPD diagnostic criteria leading to greater endorsement among sexual minority individuals, which hinders researchers’ ability to make valid group comparisons. The present study utilized an epidemiological sample of 35,995 men and women to evaluate the extent of differential item functioning (DIF) among BPD criteria across sexual orientation groups using a multiple indicators multiple causes (MIMIC) approach. All criteria except affective instability and emptiness indicated …

Meeting Diverse Student Needs: An Examination Of A Physical Education Teacher Alumnus’ Culturally Responsive Teaching Beliefs And Their Enactment, Craigory V. Nieman

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine how one physical education teacher education (PETE) alumnus teaching in a CED urban school perceives culturally responsive teaching in physical education (PE), as well as how they enact it in their classes. Guided by culturally responsive teaching and self-efficacy, this study examined John, a physical education teacher education alumnus teaching in a CED urban elementary school. John completed the culturally responsive teaching self-efficacy scale (CRTSE; Siwatu, 2007), five semi-structured interviews, five reflection journal prompts, and eight voice memos over the course of 12 weeks. Additional data collection included text messages, emails, and …

Cybersecurity: Stochastic Intensity Function And Monitoring Indicators, Hackers Demographics, Statistical Analysis And Treatment Of Ovarian Cancer, Ranju Karki

USF Tampa Graduate Theses and Dissertations

One of the major tasks in the present day-era is securing computer systems against unauthorized access. Every year we lost millions of dollars because of cyber attacks and thousands of people suffer economically and psychologically. Rapid development in the field of information technology increases the challenges to the Information technology personnel working on protecting against cyber attacks. In cyber security, vulnerabilities and hackers play a major role. Researchers are putting in enormous efforts to develop methods and models to control vulnerabilities and psychological behavior and motivation of hackers. Our study defines two important aspects of the computer operating system concerning …

Enhanced Methods In Forensic Mass Spectrometry For Targeted And Untargeted Drug Analysis, Dina M. Swanson

USF Tampa Graduate Theses and Dissertations

Forensic science has long used mass spectrometry for the analysis of drugsand metabolites. Traditionally, screening methods consisted of color tests or immunoassay and confirmation methods used gas chromatography with mass spectrometry, which was considered the gold standard for identification. In the last several decades, advances in liquid chromatography with mass spectrometry (LCMS) have brought this technology to the forefront of forensic drug identification. Drug identification in forensic drug chemistry and forensic toxicology has become increasingly challenging due to the rapid increase in illicit drug analogs including novel opioids such as fentanyl analogs. Traditional screening methodologies are often unable to identify …

Automated Approaches To Enable Innovative Civic Applications From Citizen Generated Imagery, Hye Seon Yi

USF Tampa Graduate Theses and Dissertations

Smart governance is an area, that is increasingly becoming important, not only in advanced countries, but all across the globe. Thanks to global scale network connectivity, permeance of smart-devices of various form-factors, and overall improvement in digital literary, we are now seeing "smartness" everywhere, or if not, the general public is expecting the same. Ultimately, the goal of smart governance is to facilitate state-of-the-art technologies to improve citizens’ lives. With the ubiquity of smart phone technologies today, citizens more readily participate in collaboration with public officials for improved quality of life and their communities. By utilizing optimal tools, public officials …

Untangling The Phenomenon Of Teacher Anxiety During The Covid-19 Pandemic: Voices Of Secondary Ela Teachers, Jenise Gorman

USF Tampa Graduate Theses and Dissertations

Hybrid simultaneous teaching, surgical masks, Lysol wipes, and uncertainty capture the zeitgeist of teaching during COVID-19. This study builds on teachers’ daily stressors in the classroom. Many shifts in education that never seemed possible created angst and anxiety in the classroom (Cupido, 2018; Dubey and Pandey, 2020; El Rizaq & Sarmini, 2021; Zuo et al., 2020; Garcia and Piotrowski, 2022). Teachers entered the 2020-2021 school year having to learn many firsts.The purpose of this study was to understand the interplay of work-life lived experiences of secondary English teachers with moments of anxiety during the COVID-19 pandemic. Using a post-intentional phenomenological …