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The Relationship Between B-Type Natriuretic Peptide Levels And Hospital Length Of Stay And Quality Of Life In Congestive Heart Failure Patientsthe Relationship Between B-Type Natriuretic Peptide Levels And Hospital Length Of Stay And Quality Of Life In Congestive Heart Failure Patients, Irma B. Ancheta

USF Tampa Graduate Theses and Dissertations

Previous research on quality of life (QOL) and its relation to BNP levels in heart failure (HF) has been widely studied. However, the impact of physicians' knowledge of BNP levels at time of clinic visit on QOL and hospital length of stay (LOS) has yet to be fully investigated. The purpose of this study were to determine if physicians' knowledge of BNP levels affected a change in QOL scores at 90 days and reduce hospital length of stay among heart failure patients. QOL data from HF clinic patients (N = 108, 67.5 ± 12.3, 56% male, ejection fraction 26.5 ± …

Poly(N-Isopropylacrylamide) Based Biomems/Nems For Cell Manipulation, Alexandro Castellanos Mier

USF Tampa Graduate Theses and Dissertations

In recent years, BioMEMS/NEMS have been primary elements associated with the research and development efforts in the bioengineering area. International and federal funding has effected an enormous increase in the development of state-of-the-art bioengineering and biomedical technologies. Most of the BioMEMS/NEMS related applications are associated with diagnostics, sensing and detection. Procedures for separation and manipulation of biological components play a paramount role in the function of these bioengineering mechanisms. This research was concerned with the development of a novel BioMEMS device for cell manipulation. The functioning of the device is based on the use of thermally responsive polymer networks, which …

Certificate Of Need Regulation In The Nursing Home Industry: Has It Outlived Its Usefulness?, Barbara J. Caldwell

USF Tampa Graduate Theses and Dissertations

The primary goals of the National Health Planning and Resources Development Act (P.L. 93-641) of 1974 were to (1) contain health care costs and (2) increase the accessibility and quality of health services. Certificate of need (CON) regulation is one attempt to constrain health care costs by limiting the supply of certain medical care facilities. With respect to the nursing home industry, prospective nursing home owners/operators are required to demonstrate that a "need" exists for more nursing home beds. Some States also imposed a construction moratorium that prevented any expansion of existing facilities or construction of new facilities regardless of …

Estimation Of The Particle And Gas Scavenging Contributions To Wet Deposition Of Organic And Inorganic Nitrogen, Silvia Margarita Calderã³N

USF Tampa Graduate Theses and Dissertations

Atmospheric deposition of nitrogen species represents an additional nutrient source to natural environments, and can alter the nitrogen cycle by increasing nutrient levels beyond the requirements of organisms. In Tampa Bay, atmospheric deposition of dissolved inorganic nitrogen species (DIN) has been found to be the second largest nitrogen source, but little is known about dissolved organic nitrogen species (DON). The research goal was to improve the dry and wet deposition estimates by inclusion of the DON contribution. In the atmospheric chemistry field a standard method to measure DON in atmospheric samples has not been agreed upon. This research proposes the …

Cloning And Analysis Of Putative Collagenases Of The U32 Family In Streptococcus Mutans And Streptococcus Agalactiae (Group B Streptococci), Valerie Carson

USF Tampa Graduate Theses and Dissertations

Analysis of the genomic sequences of Streptococcus mutans UA159 and Group B Streptococcus (GBS) strains Streptococcus agalactiae NEM316 and S. agalactiae 2603V/R indicated the presence of two putative collagenase genes in each organism. smcol1 from S. mutans was previously cloned and analyzed and the results indicated that the enzyme belonged to the U32 family of collagenases/peptidases. This enzyme shares homology with the prtC of Porphyromonas gingivalis, one of the principal examples of the U32 family of peptidases. Considering the potential role of these enzymes in the pathogenicity of P. gingivalis (periodontitis or gum disease), GBS (premature rupture of the amniochorionic …

Cue Reactivity And The Role Of Social Alcohol Expectancies In The College-Aged Drinking Population, Ashlee C. Carter

USF Tampa Graduate Theses and Dissertations

Research has shown alcohol expectancies to be critically important in understanding maladaptive drinking patterns within alcohol use disorders. Alcohol expectancies, thought to be automatically elicited in the presence of environmental alcohol-related cues, represent both cognitive and affective associations with drinking behavior. However, the automatic and affective properties of alcohol expectancies have not yet been thoroughly measured in the literature. Psychophysiological measures, including skin conductance, heart rate, and the acoustic startle response in particular, offer a uniquely powerful set of indices for the automatic affective processing of alcohol-related cues.

Therefore, the present study was designed to examine how alcohol expectancies moderate …

Bacteriophage For The Elimination Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Colonization And Infection, Angela Clem

USF Tampa Graduate Theses and Dissertations

Methicillin-resistant Staphylococcus aureus (MRSA) is among the most important pathogens affecting the human race in our time. In spite of recent medical advances, our therapeutic choices for MRSA infections remain limited due to the propensity of this organism to develop resistance to antimicrobials. Therefore, there is a continuing need to develop newer methods of treating MRSA infections. This dissertation examines the effects of bacteriophages 88 and 92 on ten clinical isolates of MRSA from the central Florida area. . For the majority of the MRSA isolates, bacteriophages 88 and 92 were unable to induce lysis. However, bacteriophage 88 was found …

Traumatic Loss And Transformative Life Experiences: The Lived Experience Of Green Cross Traumatologists Deployed To The New York City World Trade Center Disaster, Carron C. Cherrie

USF Tampa Graduate Theses and Dissertations

This exploratory study examines the lived experience of Green Cross traumatologists deployed to the New York City World Trade Center disaster. The deployment took place five days after the terrorist attacks on September 11, 2001. The author was a member of a ten member advance team that provided crisis stabilization services to an international union in Lower Manhattan. Disaster Mental Health Services and community outreach were provided for a month. The purpose of the study was to describe in an anthropologically holistic perspective the lived experience of traumatologists, who as Americans were also affected by the terrorist disaster. Thirty-one traumatologists …

Investigation Of Normalized Streamflow In West Central Florida And Extrapolation To Ungaged Coastal Fringe Tributaries, Kim Beth Clayback

USF Tampa Graduate Theses and Dissertations

Deriving accurate streamflow estimates for ungaged watersheds provides a challenging task for water resource engineers. Traditional methods include correlation to the nearest USGS streamflow station or numeric simulation of watershed rainfall-runoff processes. Mean annual flow, ten percent exceedance and other streamflow indices can be normalized and non-dimensionalized by dividing by the watershed drainage area and the mean annual precipitation rate. Obtaining non-dimensional parameters can be especially useful for extrapolation of flows to downstream, ungaged, coastal fringe regions. Florida and other states along the Gulf Coast exhibit strong variability in the magnitude of streamflow fraction of precipitation. The irregular patterns created …

The Association Of Head Circumference With Selected Cognitive Outcomes In Older Adults In Charlotte County, Florida, Cathleen Copenhaver

USF Tampa Graduate Theses and Dissertations

OBJECTIVE: The brain reserve hypothesis was examined in a secondary analysis of cross-sectional data from a community-based sample of 468 older adults residing in Charlotte County, Florida. The objective of the analysis was to determine the association between head circumference and eight cognitive outcomes and to assess any potential effect modification of existing associations by Apolipoprotein E (APOE) genotype. METHODS: Cognitive outcomes include scores from the Modified Mini-Mental State Exam (3MS), the Hopkins Verbal Learning Test-Revised (HVLT-R), Stroop Color-Word Test, Trail-Making Test A and B, and a word-stem completion task measuring implicit memory. Descriptive statistics were calculated for each variable. …

Behavioral And Auditory Evoked Potential (Aep) Hearing Measurements In Odontocete Cetaceans, Mandy Lee Hill Cook

USF Tampa Graduate Theses and Dissertations

Bottlenose dolphins (Tursiops truncatus) and other odontocete cetaceans rely on sound for communication, navigation, and foraging. Therefore, hearing is one of their primary sensory modalities. Both natural and anthropogenic noise in the marine environment could mask the ability of free-ranging dolphins to detect sounds, and chronic noise exposure could cause permanent hearing losses. In addition, several mass strandings of odontocete cetaceans, especially beaked whales, have been correlated with military exercises involving mid-frequency sonar, highlighting unknowns regarding hearing sensitivity in these animals.Auditory evoked potential (AEP) methods are attractive over traditional behavioral methods for measuring the hearing of marine mammals because they …

Biomechanical Evaluation Of Independent Transfers And Pressure Relief Tasks In Persons With Sci: Pilot Study, Tony J. Cresta

USF Tampa Graduate Theses and Dissertations

Persons with paraplegia who use a manual wheelchair for mobility are at high risk for overuse injuries in the upper extremities. Years of shoulder overuse performing transfers, wheelchair propulsion, dressing, bathing, and household chores, (activities of daily living or ADL) leads to an increased incidence of cumulative trauma to the shoulders. Few studies have addressed the stressful task of wheelchair transfers among SCI individuals. The goal of this pilot study is to develop valid and reliable measurement technologies to quantify shoulder musculoskeletal stressors during wheelchair transfers and pressure relief tasks among individuals with SCI. Using a standard wheelchair, 10 participants …

Risk And Protective Factors Related To Resilience In Adolescents In An Alternative Education Program, Kelly M. Crawford

USF Tampa Graduate Theses and Dissertations

Using a multivariate correlational design, this study was designed to determine the degree to which selected risk and protective factors were related to resilience in an at-risk student population. A total of 12 individual, family and external risk and protective factors were identified and data were collected from 142 6th through 11th grade students in an alternative education program for individuals who had committed severe behavioral infractions. A series of multiple regression analyses were conducted using three outcome variables: a rating of students' resilient behavior, number of behavior referrals in the students' records, and standardized reading achievement scores. Five percent …

Pain As A Motivator Of Smoking: Effects Of Pain Induction On Smoking Urge And Behavior, Joseph W. Ditre

USF Tampa Graduate Theses and Dissertations

Tobacco smoking has been associated with the development, protraction, and exacerbation of chronically painful conditions. Conversely, there is reason to believe that smokers may be motivated to use tobacco as a means of coping with their pain. To date, no controlled, experimental studies have tested for a causal relationship between pain and smoking motivation. The primary aim of the current study was to test the hypothesis that laboratory-induced cold-pressor pain would enhance smoking motivation, as measured by self-reported urge to smoke and observation of immediate smoking behavior. The effect of a smoking cue was also tested. Smokers (n = 132) …

The Commodification Of Yoga In Contemporary U.S. Culture, Michelle E. Demeter

USF Tampa Graduate Theses and Dissertations

Yoga is an increasingly visible and versatile commodity in the United States health market. Though its origins stretch to pre-Vedic India and its traditional religious purpose is linked to Hinduism, it is evident that yoga has undergone much change since its transmission to U.S. culture. In its popular, widespread incarnation in the United States, yoga is not usually learned at the feet of a guru, but at exercise centers and gyms. These secular locales of yoga's practice help define the "yoga phenomenon" in contemporary America. This phenomenon has resulted in yoga's wide acceptance and high visibility in American popular culture …

Rapid Detection Of Mycobacterium Tuberculosis In Lung Tissue Using A Fiber Optic Biosensor, Kimberly A. Denton

USF Tampa Graduate Theses and Dissertations

There is no rapid diagnostic technique at medical examiners' offices to determine if a decedent is infected with Mycobacterium tuberculosis. Present diagnostic testing requires at least one month for results. A biosensor-based sandwich immunoassay for the detection of M. tuberculosis was developed in this study. M. tuberculosis polyclonal antibody was used for target antigen capture and detection in the immunoassay. Live attenuated M. tuberculosis (ATCC 25177) suspended in phosphate-buffered saline with 0.1% Tween 20 was used as the antigen in the detection assay. The Analyte 2000 was the initial biosensor platform. Initial testing was of Freund's adjuvant complete. M. tuberculosis …

Drug Resistant Patterns Of Invasive Streptococcus Pneumoniae Infections In The State Of Florida In 2003, Michael T. Drennon

USF Tampa Graduate Theses and Dissertations

Streptococcus pneumoniae is a major bacterial pathogen which causes pneumoniae, meningitis, otitis media, and bacteremia. Currently there are two vaccines available, Pneumococcal Polysaccharide Vaccine (PPV) for adults and the Pneumococcal Conjugate Vaccine (PCV) for children. The PCV vaccine was developed in 2000 specifically for children and infants due to the ineffectiveness of the PPV vaccine in children. This is a cross sectional study of invasive S. pneumoniae in Florida during 2003. This study is designed to determine the population characteristics, clinically relevant antibiotic resistance patterns and specific risk factors for development of antibiotic resistance of invasive S. pneumoniae. Participants for …

Improving Long-Term Resettlement Services For Refugees, Asylees, And Asylum-Seekers: Perspectives From Service Providers, Kristina M. Dunman

USF Tampa Graduate Theses and Dissertations

In the United States, rights of asylum and refuge are extended to people who can prove that they have been politically persecuted. Resettlement services for refugees and asylees often focus on the short-term acquisition of employment and English language skills. These policies ignore the long-term complexities involved in reestablishing individual and group identities after war trauma and resettlement. This research is an investigation into the perspectives of service providers who work with refugees, asylees, and asylum-seekers on the need for more comprehensive, long-term services to assist in the months and years following resettlement, and on potential programs to address those …

Charaterization Of Asbestos Exposure Among Automotive Mechanics Servicing And Handling Asbestos-Containing Materials, Gary Scott Dotson

USF Tampa Graduate Theses and Dissertations

The historic use of asbestos-containing materials during the manufacturing of automobiles has resulted in a perception of an increased risk of asbestos-related pulmonary diseases within mechanics. This study was conducted to assess the potential asbestos exposures mechanics encounter while servicing vehicles assembled with parts containing asbestos, in addition to compare the cumulative lifetime asbestos exposures for different maintenance activities against theorical threshold exposures for asbestosis, lung cancer and mesothelioma. Exposure data were assembled from four independent exposure assessments performed to elucidate the airborne asbestos levels generated during the removal and replacement of brakes, gaskets, clutches and seam sealants containing asbestos. …

An Examination Of The Relationship Between The Presence Of Critical Components Of Classroom Positive Behavior Support And Student Behavior, Gregory S. Ern

USF Tampa Graduate Theses and Dissertations

This purpose of this study was to assess the relationship between the presence of classroom components of positive behavior support and student behavior outcomes. Data were collected using the Tool for Assessing Classroom Level-Positive Behavior Support (TACL-PBS) developed by Ern (2004) to assess the presence or absence of critical components of positive behavior support at the classroom level. Descriptive data on the instrument including the internal consistency, interrater agreement, and concurrent validity were analyzed and are included in this report. Forty classrooms from ten diverse elementary schools in three school districts were selected to participate in this study. Office discipline …

Vibration Level Characterization From A Needle Gun Used On U.S. Naval Vessels, Scott E. Dunn

USF Tampa Graduate Theses and Dissertations

United States (U.S.) Navy sailors are exposed to a very large number of hazards, both chemical and physical. Occupational vibration from pneumatic air tools is one of the potential exposure hazards. There are very limited data as to the exposures to one type of tool, a needle gun or needle scaler, used by the sailors.The purpose of this study was to characterize the vibration levels generated by a needle gun used in the U.S. Navy. The design of the study evaluated the difference pressure had on the acceleration levels generated from the needle scaler. Five subjects were used in the …

Monitoring And Modeling Water Quality At The C.W. Young Regional Reservoir, Daniel Robert Dye

USF Tampa Graduate Theses and Dissertations

This work explores the relationship between nutrient loading and changes in water quality in a sub-tropical, above-ground, off-stream municipal water supply reservoir, the C.W. Bill Young Regional Reservoir. The three source waters for the reservoir have varied but high levels of nutrients such as phosphorus and nitrogen. In other reservoirs, these nutrients have been linked to deterioration of water quality and increased expense in water treatment. The need to minimize excess nutrients results led to the primary research question: what allocation of withdrawals from the three sources will minimize the deterioration of water quality? To answer this question, the relationship …

Alcohol Expectancy Cognitions: Psychophysiological Perspective, Inna Fishman

USF Tampa Graduate Theses and Dissertations

Considerable evidence indicates that the expectations individuals hold about the effects of alcohol determine, to a degree, the amount of alcohol they drink. However, the bulk of this evidence was acquired using verbally-based measures of expectancy. The present study sought to extend the validation network by utilizing an electrophysiological measure -- the P300 component of the Event Related Potentials (ERPs) --

which is thought to index fundamental neurophysiological processes sensitive to expectancy.Previous research has demonstrated that, when presented with various outcomes of alcohol consumption, heavier drinkers endorse statements that assert positive and arousing effects of alcohol, while lighter drinkers endorse …

Nurse Exposure To Waste Anesthetic Gases In A Post Anesthesia Care Unit, Larry A. Flack

USF Tampa Graduate Theses and Dissertations

It has been estimated that over 200,000 healthcare professionals may be exposed to waste anesthetic gases and are at risk of occupational injury. In 1977, the National Institute for Occupational Safety and Health (NIOSH) issued the publication: Criteria for a Recommended Standard....Occupational Exposure to Waste Anesthetic Gases and Vapors. This publication was based primarily on scientific evidence from human and animal studies suggesting that chronic exposures to anesthetic gases increases the risk of both spontaneous abortion and congenital abnormalities in offspring among female workers and wives of male workers exposed to waste anesthetic gases. In this recommended standard, NIOSH defines …

Functional Topology And Regulation Of Endothelial Nitric Oxide Synthase And Associated Caveolar Components, Brenda R. Flam

USF Tampa Graduate Theses and Dissertations

The discovery of nitric oxide (NO) as the endothelial-derived relaxing factor has led to significant research on NO and the proteins involved in its function, generation, location and regulation. Synthesis of NO by blood vessel endothelial cells results from the enzymatic oxidation of arginine by endothelial nitric oxide synthase (eNOS) resulting in the formation of equimolar amounts of NO and citrulline. Citrulline is sequentially recycled to arginine by successive reactions involving the enzymes argininosuccinate synthase (AS) and argininosuccinate lyase (AL), respectively.

eNOS activity has been shown to be regulated by post-translational modifications including dynamic phosphorylation on multiple serine/threonine and tyrosine …

Development Of A Microsphere-Based Immunoassay For The Detection Of Igm Antibodies To West Nile Virus And St. Louis Encephalitis Virus In Sentinel Chicken Sera, Logan C. Haller

USF Tampa Graduate Theses and Dissertations

West Nile virus (WNV) and St. Louis Encephalitis (SLEV) are arthropod-borne viruses belonging to the genus Flavivirus and are classified as significant human pathogens of global epidemiological importance. Since its introduction into the United States in 1999, WNV has spread throughout most of the country and has caused major epidemics of neuroinvasive disease (Hayes and Gubler, 2005). SLEV is endemic to the United States and is maintained in an enzootic transmission cycle in Florida.The Florida Sentinel Chicken Arboviral Surveillance Network was established in 1978 following a widespread rural epidemic of SLEV in central Florida to monitor the activity of arboviruses …

Linking Pretreatment Therapist Characteristics To The Therapeutic Alliance In Youth Treatment: An Examination Of Professional Burnout, Counseling Self-Efficacy And Gender Role Orientation, Jessica B. Handelsman

USF Tampa Graduate Theses and Dissertations

The present study investigated three pretreatment therapist characteristics (professional burnout, counseling self-efficacy, and gender role orientation) in relation to the therapeutic alliance within the context of youth treatment. It was hypothesized that the emotional exhaustion and depersonalization dimensions of burnout would be negatively associated with the alliance, while the personal accomplishment dimension of burnout and counseling self-efficacy would be positively associated with the alliance. In addition, it was hypothesized that androgynous therapists would have superior alliances, relative to stereotypically masculine or feminine therapists. Participants were 42 pairs of therapists and youth clients. Prior to intake, therapists completed the Maslach Burnout …

Respondent Fatigue In Self-Report Victim Surveys: Examining A Source Of Nonsampling Error From Three Perspectives, Timothy C. Hart

USF Tampa Graduate Theses and Dissertations

Survey research is a popular methodology used to gather data on a myriad of phenomena. Self-report victim surveys administered by the Federal government are used to substantially broaden our understanding of the nature and extent of crime. A potential source of nonsampling error, respondent fatigue is thought to manifest in contemporary victim surveys, as respondents become "test wise" after repeated exposure to survey instruments. Using a special longitudinal data file, the presence and influence of respondent fatigue in national self-report victim surveys is examined from three perspectives. Collectively, results provide a comprehensive look at how respondent fatigue may impact crime …

Confronting College: Foster Care Youth Deciding Whether To Participate In Higher Education Programs, Linda K. Herlocker

USF Tampa Graduate Theses and Dissertations

This study's purpose was to explore the college choice process for foster care youth who are aging out of Florida's protective services system. The research methodology included three components. First, a survey of the Independent Living Coordinators throughout the state of Florida solicited data regarding participation rates and enrollment patterns among foster care youth. Next, in a meeting setting, a survey was administered to foster care youth, probing the extent to which they considered certain college choice decision factors. Finally, upon completion of the survey, participants remained for a guided focus group discussion to further explore their decision criteria.The results …

The Early Time Course Of Smoking Withdrawal Symptoms, Peter Schuyler Hendricks

USF Tampa Graduate Theses and Dissertations

Despite the large volume of research on tobacco withdrawal, the vast majority of studies have focused on the onset and remission of symptoms over the course of several days and weeks, with the earliest assessment periods occurring the day after cessation. To date, there has been no systematic study of the very early time course of the tobacco withdrawal syndrome, despite its obvious relevance to the maintenance of both smoking and postcessation abstinence. The published literature contains a range of estimates about the early appearance of withdrawal symptoms, but without reference to empirical data. The main objective of the current …