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Evaluation Of Taqman Real-Time Pcr For The Detection Of Viable Cryptosporidium Parvum Oocysts In Environmental Water Samples, Melissa A. Cameron

USF Tampa Graduate Theses and Dissertations

Cryptosporidium parvum is of growing public health concern due to its ability to survive typical water treatment processes. In order to protect the public from infection, the Environmental Protection Agency developed Method 1623 for the detection of Cryptosporidium oocysts in environmental water samples. Execution of this method is time consuming, and the results do not provide an accurate estimation of viability. Therefore, current research is focused on creating a real-time PCR method for the accurate detection of viable Cryptosporidium parvum in environmental water samples.

This thesis presents the development of a real-time PCR method, and the results obtained in its …

Community Context And Health Disparities Among Older Adults, Helen M. Zayac

USF Tampa Graduate Theses and Dissertations

African Americans, Hispanics, and other minorities in the U.S. continue to face conditions of residential and educational segregation, lower socioeconomic status, and higher rates of mortality than whites. Better theory-based research that uses community and individual level factors to explain how health disparities are created and perpetuated is needed. The Community Context and Health Disparities Model, which extends the work of Schulz and Northridge (2004) with elements described by Williams and Collins (2001), is described. This framework identifies the pathways by which characteristics of the physical, built, social, economic, and healthcare environments impact health and are mediated by individual traits. …

Measurement Equivalence Of The Center For Epidemiological Depression Scale In Racially/Ethnically Diverse Older Adults, Giyeon Kim

USF Tampa Graduate Theses and Dissertations

This dissertation study was designed to examine measurement equivalence of the Center for Epidemiological Studies Depression (CES-D) Scale across White, African American, and Mexican American elders. Specific aims were to identify race/ethnicity-, sociodemographic-, and acculturation and instrument language-related measurement bias in the CES-D. Three studies were conducted in this dissertation to accomplish these aims. Two existing national datasets were used: the New Haven Established Populations for Epidemiologic Studies of the Elderly (EPESE) for the White and African American samples and the Hispanic Established Populations for Epidemiologic Studies of the Elderly (H-EPESE) for the Mexican-American sample. Differential item functioning (DIF) analyses …

The Art Of Existentialism: F. Scott Fitzgerald, Ernest Hemingway, Norman Mailer And The American Existential Tradition, J'Aimã© L. Sanders

USF Tampa Graduate Theses and Dissertations

The purpose of my research is to examine the philosophic influences on three literary works: F. Scott Fitzgerald's The Great Gatsby, Ernest Hemingway's Death in the Afternoon, and Norman Mailer's An American Dream. Through an investigation of biographical, historical, cultural, and textual evidence, I will argue for the influence of several European philosophers---Friedrich Nietzsche, Søren Kierkegaard, and Martin Heidegger---on these authors and on the structures and messages of their works. I will discuss how the specific works I have selected not only reveal each author's apt understanding of the existential-philosophical crises facing the individual in the twentieth century, but also …

Src Kinase Inhibitors For The Treatment Of Sarcomas: Cellular And Molecular Mechanisms Of Action, Audrey Cathryn Shor

USF Tampa Graduate Theses and Dissertations

Sarcomas are rare mesenchymally-derived tumors with limited treatment options. Tyrosine kinases may serve as potential targets for sarcoma therapy because many are mutated or overexpressed in sarcomas and cell lines. One potential molecular target for sarcoma treatment is the Src tyrosine kinase. Three independently synthesized Src kinase inhibitors were evaluated in human sarcoma cell lines. Of the three, dasatinib, provided promising results as a potential sarcoma therapy. Until this study, dasatinib activity had not been characterized in sarcoma cells. Based on our previous findings of Src activation in human sarcomas, we evaluated the effects of dasatinib in twelve sarcoma cell …

Scissors Paper Rock, Barbara St. Clair

USF Tampa Graduate Theses and Dissertations

Scissors Paper Rock is based on the true story of Amy Biel, a young and idealistic American woman who went to South Africa to support the political effort against Apartheid. She worked there as a volunteer organizer during a time of great political turmoil, and was days away from returning home to the United States, when she was violently killed in a riot. While Amy's death was not an accident--four men were found guilty for taking her life--it is certainly possible to think of her death as a tragedy. Amy's idealism and belief in her power to do good, allowed …

Cellular Electrofusion Utilizing Corona Fields And Dc Pulse Technology, Joshua Stein

USF Tampa Graduate Theses and Dissertations

Cell fusion is an important technique that is used in the field of medicine and biomedical research. For instance, fusion can be used to create hybridomas and novel types of secretory hybrid cells. It may also be used to engineer cultured insulin-secreting pancreatic B-cell lines for the treatment of diabetes. Historically, the applications listed above have been accomplished by a number of methodologies including dielectrophoresis, centrifugation, polyethylene glycol (PEG) and viral fusion proteins. However, these approaches often fail to produce the desired results due to poor cell viability, lack of 1:1 fusion, and use of non-physiological environments. It is proposed …

Enduring Changes In Reward Mechanisms After Developmental Exposure To Cocaine: The Role Of The D2 Receptor, Kirstie H. Stansfield

USF Tampa Graduate Theses and Dissertations

During adolescent brain maturation, there are likely sensitive periods where environmental conditions, including drug exposure, may influence development by modifying neuronal connections. Altering neuronal function may produce different phenotypes than expected under normal conditions that may influence subsequent responding to drugs of abuse after the brain is fully mature. Experiment one investigated the relationship between novelty preference and cocaine place preference in adolescent and adult rats. High responding adolescent rats displaying greater free choice novelty exploration (but not forced novelty locomotion) expressed decreased cocaine place conditioning compared to low responding rats. No relationship was found in adult rats. Experiment two …

Clinical Supervision Of Child And Adolescent Counselors In Residential Foster Care: A Collective Case Study, Lee A. Teufel

USF Tampa Graduate Theses and Dissertations

A collective case study is the study of more than one case (Stake, 1995). One therapist supervisor and three therapist supervisees from a child and adolescent residential foster care facility were observed during their individual clinical supervision and interviewed post-supervision. Currently, the literature on clinical supervision seldom addresses the supervision of working professional counselors; particularly those who are child and adolescent counselors (CACs).

Using a qualitative approach, two fundamental questions guided this inquiry: (a) what is the nature of clinical supervision involving a supervisor who provides clinical supervision to counselors in a child and adolescent residential foster care center that …

Job Satisfaction Among High School Assistant Principals In Seven Florida Counties, John R., Taylor

USF Tampa Graduate Theses and Dissertations

This study replicated the study by Mary E. Neal, Job Satisfaction of Florida's High School Assistant Principals as a Factor in the Maintenance of an Administrative Workforce (2002) and extended the research by examining the job satisfaction of high school assistant principals in seven Florida county school districts. The present study utilized quantitative and qualitative data. Respondents (n = 128) were surveyed using the Minnesota Satisfaction Questionnaire (MSQ short form), Individual Demographic Questionnaire, and Telephone Interview Questionnaire. One assistant principal from each of seven counties volunteered to complete the Telephone Interview Questionnaire. The 128 respondents represented 60% of 214 assistant …

Uncertainty In The Information Supply Chain: Integrating Multiple Health Care Data Sources, Monica Chiarini Tremblay

USF Tampa Graduate Theses and Dissertations

Similar to a product supply chain, an information supply chain is a dynamic environment where networks of information-sharing agents gather data from many sources and utilize the same data for different tasks. Unfortunately, raw data arriving from a variety of sources are often plagued by errors (Ballou et al. 1998), which can lead to poor decision making. Supporting decision making in this challenging environment demands a proactive approach to data quality management, since the decision maker has no control over these data sources (Shankaranarayan et al. 2003). This is true in health care, and in particular in health planning, where …

Narrative Efforts At Social Redemption By People With Aids/Hiv, Andrea Zolnier Thelen

USF Tampa Graduate Theses and Dissertations

This dissertation explores four narrative texts written about AIDS/HIV and evaluates each one by applying Kenneth Burke's redemption drama, consisting of guilt, purification, and redemption. The methodology is a close textual analysis using rhetorical analysis as a way to highlight the use of the redemption drama in language. The first chapter explores the history of AIDS/HIV and makes the argument for using Burke's rhetorical approach. The second chapter briefly highlights the plot of the four narratives and provides background information and context for each book. The third chapter applies the concept of guilt to all four narratives. The fourth chapter …

Brevetoxins In Marine Birds: Evidence Of Trophic Transfer And The Role Of Prey Fish As Toxin Vector, Michelle Van Deventer

USF Tampa Graduate Theses and Dissertations

Harmful algal blooms (HABs) of the brevetoxin-producing dinoflagellate Karenia brevis occur periodically along the central west coast of Florida. Mass mortalities of marine birds have long been associated with these blooms, yet there is little data documenting the accumulation of brevetoxins in the tissues of birds and their prey items. An intense HAB event impacted the region from Tampa Bay to Charlotte Harbor during most of 2005. More than one hundred marine birds, representing twenty three species, were collected during this bloom. All birds sampled were found dead or had died within 24 hours of admittance to local wildlife rehabilitation …

Local Strategies In A Global Network: Disability Rights Advocacy In Jamaica, Joan A. Tucker

USF Tampa Graduate Theses and Dissertations

The declaration of the International Year of Disabled Persons in 1981 and the international activism of persons with disabilities resulted in the globalization of disability, which involved a new framework for the treatment of PWDs. Although disability activism was being played out globally, it has been about PWDs gaining equality and full participation in their local societies. Therefore, at the heart of an international movement was the strategic organization of PWDs, most of who live in the developing world. This dissertation is based on a case study of activism for disability rights in one particular developing society, Jamaica. The Jamaican …

Defining Service Quality In An Outpatient Clinic With Complex Constituency, Swati Verma

USF Tampa Graduate Theses and Dissertations

The 2001 Institute of Medicine's (I.O.M.) landmark report, Crossing the Quality Chasm: A New Health System for the 21st Century observes that, "[though] medical science and technology have advanced at a rapid pace,...the health care delivery system has floundered in its ability to provide consistently high-quality care" (I.O.M. 2001). The report recommended six quality aims for a twenty-first century health care system; one of them being patient-centered care. It explains patient-centered care as "providing care that is respectful of and responsive to individual patient preferences, needs, and values and ensuring that patient values guide all clinical decisions" (I.O.M. 2001). This …

The Relationship Between School Connectedness And Bullying Vicitmization In Secondary Students, Janet Urbanski

USF Tampa Graduate Theses and Dissertations

Bullying is a complex behavior that can cause academic and social problems for students and can contribute to a negative school climate. Students who feel isolated or do not feel connected to their school may experience similar risks to those who are victimized by peers. Recent school violence incidents have led to an increase in bullying behavior research. The importance of the school climate is also emerging in educational discourse prompting a growth of research in school connectedness and positive relationships. However, research on the impact that relationships and school connectedness may have on bullying victimization at school is limited. …

Effect Of A Preoperative Warming Intervention On The Acute Phase Response Of Surgical Stress, Vanda Doreen Wagner

USF Tampa Graduate Theses and Dissertations

When a patient is exposed surgical stress, the endocrine system secretes hormones in response to that stress. These hormones further activate the immune system to release cytokines and other acute phase reactions. These processes are supposed to protect the body by upregulating the innate immune system and producing an inflammatory response that acts to protect and heal. However, uncontrolled surgical stress may cause a weaker immune response that may lead to delayed wound healing. The phenomenon of unplanned perioperative hypothermia is known to expose patients to additional surgical stress. The purpose of this preliminary experimental study was to determine the …

Subtyping Psychopathy: Exploring The Roles Of Degree Of Punishment, Cognitive Dissonance And Optimism, John M. Weir

USF Tampa Graduate Theses and Dissertations

For over half of a century, social and behavioral scientists have been investigating the construct of psychopathy. Even so, psychopathy is still a highly misunderstood personality construct. Even though it has been estimated that psychopaths make up only about 1% of the general population, they are believed to consist of 15-25% of the prison population (Hare, 1996). However, not all psychopaths are in prison. Psychopaths can also be found in such fields as the legal profession, the business world and in politics (Babiak, 1995). In terms of criminal behaviors, psychopaths are arrested at earlier ages, have a higher rate of …

The Influence Of Masculinity Ideology On High-Risk Sexual Behavior Among Men Who Have Sex With Men, Christopher Wheldon

USF Tampa Graduate Theses and Dissertations

Epidemiologic evidence shows that the incidence of HIV among men who have sex with men (MSM) is on the rise. high-risk sexual behaviors are the primary mode of transmission and are responsible for increased rates of infection. a growing body of evidence suggests that endorsement of normative beliefs and values regarding manhood and masculinity, or masculinity ideology, may help to explain certain high-risk sexual behaviors. this study investigated the associations between a measure of masculinity ideology and sexual risk taking among a sample of MSM. in addition, indirect pathways in which masculinity ideology may lead to sexual risk taking were …

Pneumatic Tool Hand-Arm Vibration And Posture Characterization Involving U.S. Navy Shipboard Personnel, Charles R. Wilhite

USF Tampa Graduate Theses and Dissertations

The United States Navy incorporates many different occupations to ensure it achieves its overall mission. These occupations are extremely diversified and present a wide spectrum of occupational exposures. Many of these exposures have been well studied and documented. However, shipboard pneumatic tool hand-arm vibration, (HAV) and how it relates to different body postures is an area of occupational exposure that has received little attention. The chief objective of this study was to assess whether there is a difference in hand-arm vibration levels, while working on one of two surface orientations (e.g., horizontal and vertical) among distinctly different pneumatic tools while …

Graduate Students/Mothers Negotiating Academia And Family Life: Discourses, Experiences, And Alternatives, Shirlan A. Williams

USF Tampa Graduate Theses and Dissertations

This study examines the experiences and perceptions of graduate students who are mothers. Based on discourse analysis, one-on-one interviews, and focus groups, this study argues that institutional discourses and practices place graduate students who are mothers in untenable and irreconcilable positions between "mom" and "student," between "family" and "the academy," between "self" and "success." These irreconcilable differences are created in discourse, enacted in institutional policies, and lived by the participants. The study offers action-based alternatives to the status quo policies of the university based on the self-described needs of these women. It reveals that support from all fronts--the home, the …

Technology And Older Faculty: A Descriptive Study Of Older Florida Community College Faculty, Christopher D. Van Der Kaay

USF Tampa Graduate Theses and Dissertations

Institutions of higher learning across the United States are experiencing an aging faculty population. A significant proportion of college and university faculty are over 55, a growth expected to continue in future years. Parallel to this growth and change has been an expanding use of technology in higher education. Despite this trend and potential implications, few studies have provided in-depth insight into older faculty and technology. The study used a quantitative descriptive design to provide a comprehensive look at older community college faculty and various aspects of technology. Areas examined included older faculty's perceptions of technology, their attitudes toward institutional …

Hospitalizations Associated With Pneumococcal Infection Within The Medicare Population Among Vaccinated And Non-Vaccinated Patients, Silky Fanyelle Webb

USF Tampa Graduate Theses and Dissertations

BACKGROUND: Streptococcus pneumoniae is the primary causative agent of pneumonia in older adults. Vaccination is the only tool to protect against pneumococcal infection; however, vaccination rates remain far below the Healthy People 2010 objective of 90% coverage. The number one reason for such low rates is attributed to controversy over the protective efficacy of the vaccine in preventing nonbacteremic pneumonia (eg, community-acquired pneumonia [CAP]). OBJECTIVES: The primary objectives of this study were to assess the incidence of pneumonia, pneumonia requiring hospitalization, and pneumonia hospitalization costs. METHODS: In this retrospective cohort study of Medicare beneficiaries aged 65 years in 2003, subjects …

Effect Of Personal And Practice Contexts On Occupational Therapists' Assessment Practices In Geriatric Rehabilitation, Mirtha Montejo Whaley

USF Tampa Graduate Theses and Dissertations

Despite considerable debate surrounding an age associated cognitive decline in non-demented elders, recent studies indicate that changes attributable to normal aging affect cognitive processes and fluid abilities. Additionally, studies indicate that factors such as physical illness, depression, neurological damage, medication side effects, drug interactions, and the effects of surgery and anesthesia may also cause varying degrees of cognitive impairment. Impairment of cognitive function is known to affect treatment and rehabilitation outcomes for older persons, and increase their likelihood of institutionalization. Although proper screening and identification of cognitive deficits in geriatric patients are crucial in developing treatment plans, there is evidence …

A New Questionnaire To Determine The Frequency And Severity Of Symptoms Caused By Inhaled Odors, Chemicals And Irritants In Normal Subjects And Their Relation To Health-Related Quality Of Life, Stephen E. Williamson

USF Tampa Graduate Theses and Dissertations

Individuals may develop symptoms in response to inhaled odors, chemicals, and irritants. This may affect their quality of life. Little is known about the prevalence and severity of symptoms that result from exposure to odors, chemicals and irritants. This study demonstrates the development of a new respiratory questionnaire to detect the prevalence and severity of symptoms experienced upon exposure to chemicals, odors, and irritants, and relates these symptoms to quality of life. This questionnaire was submitted to 96 volunteers at the University of South Florida College of Public Health who responded to items regarding symptoms developed in response to exposure …

Caregivers’ Perceptions Of The Effectiveness Of The Helping Our Toddlers, Developing Our Children’S Skills Parent Training Program: A Pilot Study, Jillian Leigh Williams

USF Tampa Graduate Theses and Dissertations

This study was designed to evaluate a parent training curriculum: Helping Our Toddlers Developing Our Children's Skills (HOT DOCS© ) using archival data collected between August 2006 and April 2007. The evaluation studied the impact of specific components of the parent training program on both participants' knowledge and attitudes and their perceptions of targeted children's behavior. One-hundred-forty-six caregivers of children between the ages of 14 months and ten years of age participated in the parent training program and were included in the analyses. Measures included a pre/post knowledge test, rating scales of child problem behavior, weekly progress monitoring forms for …

Comparison Of Student Outcomes In Distance Learning Internships Versus Traditional Dietetic Internships, Lauri Y. Wright

USF Tampa Graduate Theses and Dissertations

One way in which higher education is responding to technology advances, demographics changes, and economic pressures is through the development of distance learning. Distance learning represents one of the most prominent trends in higher education today. Understanding the impact of this technologically driven change on student outcomes is unmistakably important. One example of this trend in higher education is the distance learning internship in dietetic training programs. The purpose of this study was to compare student outcomes in distance learning dietetic internships to student outcomes in traditional dietetic internships. The pass rate of the registration exam for dietitians, levels of …

The Impact Of Organizational Climate Variables Of Perceived Organizational Support, Workplace Isolation, And Ethical Climate On Salesperson Psychological And Behavioral Work Outcomes, Robert J. Riggle

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation is to build and test a model that integrates the marketing, management, and psychological literature with respect to organizational climate variables and their direct and indirect impact on salesperson psychological and behavioral outcomes, as well as one that answers the overarching research question of how organizational climate variables impact salesperson psychological and behavioral work outcomes. Data were collected during the time period from April 2006 until May 2006. Three hundred survey invitations were sent via e-mail to salespeople at three organizations. The participating organizations included a privately owned publishing firm located in the southeastern United …

Manifestations Of Ebenezer Howard On Disneyland, Michelle M. Rowland

USF Tampa Graduate Theses and Dissertations

While political praise and condemnation of Disney is commonplace in the literature, my research will focus instead on the origination of Disney's design plan for Disneyland and the theoretical and physical connections between key historical figures and the finished product. I will not consider what Disneyland means to the world today---that is a subject many others have covered, some even brilliantly; instead, I will consider what social concepts contributed to the initial design in an attempt to see the underlying values at work in this post-modern utopia. In this thesis, I intend to show that Walt Disney's initial design for …

The Effect Of Thin-Slicing On Structured Interview Decisions, Gregory F. Schmidt

USF Tampa Graduate Theses and Dissertations

This study examined whether hiring recommendations based on thin slices of a structured interview were associated with recommendations based on the interview in its entirety. After viewing 12 seconds of silent interviewee behavior, participants made hiring recommendations that were significantly correlated with those produced by individuals viewing a still-frame of the interview and the entire interview. In an effort to determine what sources of information participants were using to arrive at their recommendations, nonverbal behaviors were examined in detail. Applicants who appeared attentive, not anxious, competent, confident, dominant, optimistic, and professional were more likely to receive positive hiring recommendations than …