Digital Commons Network^™

Plasma Mediated Molecular Delivery, Richard J. Connolly

USF Tampa Graduate Theses and Dissertations

Non-viral delivery of plasmid DNA has traditionally relied upon physical forces applied directly to target tissues. These physical methods typically involve contact between an applicator and the target tissue and often cause transient patient discomfort. To overcome the contact-dependent limitations of such delivery methodologies, an atmospheric direct current plasma source was developed to deposit ionized gas molecules onto localized treatment sites. The deposition of charged species onto a treatment site can lead to the establishment of an electric field with strengths similar to those used for traditional electroporation. In vitro experiments proved that this technology could transiently permeabilize cell membranes …

More Than Bows And Arrows: Subversion And Double-Consciousness In Native American Storytelling, Anastacia M. Schulhoff

USF Tampa Graduate Theses and Dissertations

W. E. B. Du Bois‘ legendary reflections on the ―peculiar sensation, this double-consciousness, this sense of always looking at one‘s self through the eyes of others‖ has been applied almost exclusively to the souls of African American people (Du Bois 1903). This thesis shows how the concept of double-consciousness is alive in the stories told by Native Americans. I draw upon data from two websites that have recorded the stories told by ―exemplary indigenous elders, historians, storytellers and song carriers‖ and their oral traditions that serve the ―purpose of cultural preservation, education, and race reconciliation‖ (Wisdom of the Elders, 2009). …

Exploring The Relationships Among Work-Related Stress, Quality Of Life, Job Satisfaction, And Anticipated Turnover On Nursing Units With Clinical Nurse Leaders, Mary Kohler

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to explore the relationship of the Clinical Nurse Leader (CNL) (AACN) role with the variables of work related stress, quality of life, job satisfaction and anticipated turnover of acute care nurses. Participants included registered nurses (RNs) (N= 94) in Florida recruited from 3 (not for profit) Magnet hospitals in the Tampa Bay Florida area. An ex post facto design was used to test the hypotheses of this study; independent t-tests compared RN’s responses on survey tools measuring work-related stress, quality of life, job satisfaction, and anticipated turnover. Multiple regression analysis was used to examine …

Enhancing Virus Surveillance Through Metagenomics: Water Quality And Public Health Applications, Karyna Rosario-Cora

USF Tampa Graduate Theses and Dissertations

Monitoring viruses circulating in the human population and the environment is critical for protecting public and ecosystem health. The goal of this dissertation was to incorporate a viral metagenomic approach into virus surveillance efforts (both clinical and water quality control programs) to enhance traditional virus detection methods.

Clinical surveillance programs are designed to identify and monitor etiological agents that cause disease. However, the ability to identify viruses may be compromised when novel or unsuspected viruses are causing infection since traditional virus detection methods target specific known pathogens. Here we describe the successful application of viral metagenomics in a clinical setting …

Rethinking Ethical Naturalism: The Implications Of Developmental Systems Theory, Jared J.. Kinggard

USF Tampa Graduate Theses and Dissertations

Biological research has the capacity to inform ethical discussions. There are numerous questions about the nature of sexual orientation, intelligence, gender identity, etc., and many of these questions are commonly approached with the benefit of implicit or explicit biological commitments. The answers to these sorts of questions can have a powerful impact on social, ethical, and political positions. In this project I examine the prospect of naturalizing ethics under the umbrella of developmental systems theory (DST). If one is committed to DST, then those ideas involved in DST that steer biological research will also have implications for ethics. There has …

Puerperal Fever In Britain: Failed Models Of Disease Causation, Jessica Wells

USF Tampa Graduate Theses and Dissertations

In eighteenth- and nineteenth-century Britain, a bacterial infection which we now

know to be caused primarily by a streptococcus, was killing women in childbirth at an

alarming rate. The disease, called puerperal, or childbed, fever, was being transmitted

primarily from doctor to patient by a doctor’s unwashed hands and filthy, contaminated

clothing and linens. Despite this evident and, in retrospect, obvious vector, the doctors of

this period never discovered how to prevent their patients from dying a gruesome and

painful death. Many physicians wrote extensive accounts of the illness but often ended

their works in despair, unable to find the …

Evaluation Of Oncology Nurses' Knowledge, Practice Behaviors, And Confidence Specific To Chemotherapy Induced Peripheral Neuropathy, Rebecca Denise Mcallister

USF Tampa Graduate Theses and Dissertations

Chemotherapy induced peripheral neuropathy (CIPN) remains one of the most serious and challenging symptoms oncology nurses encounter in caring for patients receiving neurotoxic chemotherapy. CIPN is under-addressed, under-reported, and symptoms are minimized by healthcare providers, which adversely affect patient quality of life, physical function, and emotional well-being. There is an absence of research examining nurses’ knowledge and practice behaviors related to CIPN. The purpose of this study was to explore oncology nurses knowledge, practice behaviors, confidence, and the relationship between education, experience, and knowledge specific to CIPN.

Data was collected at Oncology Nursing Society (ONS) Chapter meetings throughout central and …

Examination Of The Effect Of Child Abuse Case Characteristics On The Time A Caseworker Devotes To A Case, Christopher J. Card

USF Tampa Graduate Theses and Dissertations

This study used an explanatory research model that determined the effect on caseworker time and therefore workload caused by specific characteristics of cases assigned after the child abuse investigation is complete. The purpose of this study was to explain the relationship between child protection case characteristics and the time an assigned caseworker devotes to a case. With this knowledge an informed methodology to assess the current workload of a caseworker could be used to assure that the caseworker is able to successfully complete the tasks required for each child assigned. Further, the knowledge of the amount of time spent on …

Characterization Of The Role Of Nicotine And Delta 9-Thc In Modulation Of Neuroinflammation, Jared C. Ehrhart

USF Tampa Graduate Theses and Dissertations

Neuroinflammation is a major driving force in the progression of neurodegenerative disorders. Nicotinic acetylcholine receptors, as well as cannabinoid CB2 receptors, have been shown to have strong anti-inflammatory properties when activated. These effects are shown, in vivo, to be a result of stimulation of α7 nAChRs and CB2 cannabinoid receptors. Microglia cells, an immune cell in the brain, are shown to express both of these receptor subtypes. The studies detailed herein, investigated the ability of two compounds, nicotine and Δ9-THC, in modulation of inflammatory processes. Stimulation of these receptors on microglia using nicotine and Δ9-THC blocked the activation of these …

The Use Of Proteomic Techniques To Study The Physiology And Virulence Of Staphylococcus Aureus, Frances Rivera

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus

is a bacterial pathogen that is believed to be the most common agent of human infectious disease, causing conditions ranging from common skin lesions to life-threatening illnesses. S. aureus has also shown a remarkable ability to develop resistance to antimicrobial treatment, making infections difficult to treat. In the post-genomic era, proteomic studies analyzing the protein complement of a genome in a particular organism at any given time, have gained real significance. This result is largely due to dynamic changes in protein expression profiles which can lead wide alterations in physiology and behavior. For proteomics, it is necessary to …

Does Patient Dementia Limit The Use Of Cardiac Catheterization In St-Elevated Myocardial Infarction?, Marianne Chanti-Ketterl

USF Tampa Graduate Theses and Dissertations

Regardless of age or mental capacity, percutaneous coronary intervention (PCI) is the first line of treatment for ST-elevated myocardial infarction (STEMI). This study evaluates the disparities in the use of diagnostic cardiac catheterization and PCI in STEMI patients with dementia. A retrospective analysis was performed of Florida‟s comprehensive inpatient surveillance system for the years 2006-2007 with admission diagnosis of STEMI. Logistic regression analysis was used to identify disparities in the use of intervention among all STEMI patients. A total of 8,331 STEMI patients met the inclusion criteria. Of these, 77% were catheterized and of these 67% received PCI. A total …

Design And Fabrication Of A Membrane Integrated Microfluidic Cell Culture Device Suitable For High-Resolution Imaging, Alla A. Epshteyn

USF Tampa Graduate Theses and Dissertations

Malaria remains a serious concern for people living and traveling to warm climates in Africa, Asia, and some parts of America. Understanding the mechanism of the malaria parasite in the liver phase could lead to important discoveries for preventative and treatment therapeutics before the disease develops into the blood stage. While in vitro liver cell culture models have been explored, a device that mimics the liver cell architecture with the capability of high-resolution imaging has never been created. In this research, a cell culture microfluidic device was designed and fabricated with a membrane integrated design to mimic the architecture of …

The Design And Synthesis Of Functionalized Porphyrins And Their Applications In Group Transfer Reactions, Medicine, And Materials, Kimberly Bliss Fields

USF Tampa Graduate Theses and Dissertations

Porphyrins and their analogs are a class of chemically and biologically important compounds that have found a variety of applications in different fields such as catalysis, medicine, and materials. The physical, chemical, and biological dependence of the peripheral substituents of porphyrins on their properties has prompted great effort towards the synthesis of new porphyrins with different electronic, steric, and conformational environments. To this end, porphyrins have been prepared using a modular approach from bromo- and triflate synthons. These synthons underwent palladium-catalyzed cross-coupling with chiral amines, amides, alcohols, and boronic esters to create products that were tested for biological activity.

Metalloporphyrins …

Analysis Of Stochastic Disruptions To Support Design Of Capacitated Engineered Networks, Andrés Fernando Uribe-Sánchez

USF Tampa Graduate Theses and Dissertations

This work is a compilation of four manuscripts, three of which are published and one is

in the second round of review, all in refereed journals. All four manuscripts focus on analysis

of stochastic disruptions to support design of capacitated engineered networks. The work

is motivated by limited ability to mitigate elevated risk exposure of large-scale capacitated

enterprise networks functioning in lean environments. Such inability to sustain enterprise

capacity in the face of disruptions of various origins has been causing multi-billion enterprise

forfeitures and hefty insurance premiums. At the same time, decision support methodologies

for reliable design of dynamic capacitated …

Phenotypic And Genotypic Analysis Of In Vitro Selected Artemisinin Resistant Plasmodium Falciparum, Matthew Scott Tucker

USF Tampa Graduate Theses and Dissertations

Artemisinin and its derivatives provide faster clearance of parasitemia than any other antimalarial drugs and these drugs are part of frontline combination therapies in areas where drug-resistant Plasmodium falciparum exists. Clinical resistance to artemisinins is emerging on the Thailand-Cambodia border, making it imperative to investigate mechanisms of artemisinin resistance. Previous work in our laboratory showed ring-stage parasites enter a dormant state after exposure to artemisinin. We hypothesize that this period of dormancy is directly related to recrudescence and prolonged parasite clearance times in patients, and possibly resistance. The target of artemisinin is currently unknown, and potential resistance mechanisms are not …

Gender And Cocaine Use Influence The Expression Of Urinary Markers Of Inflammation And Oxidative Stress, Marie Meagher Bourgeois

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to investigate whether or not gender differences may be present in the expression of a number of urinary proteins which may serve as markers of inflammation and oxidative stress. Males and females have different patterns of illness and different life spans, suggesting basic biological traits exert significant control on the incidence of rhabdomyolysis, renal failure, atherosclerosis, myocardial ischemia, myocardial contraction band formation, autoimmune disorders and general inflammatory diseases. Men are at greater risk for cardiovascular disease; however women, particularly elderly women, have higher fatality rates due to heart failure. Renal diseases progress far more …

Impact Of Tank Material On Water Quality In Household Water Storage Systems In Cochabamba, Bolivia, Cynthia Anne Schafer

USF Tampa Graduate Theses and Dissertations

The importance of water as a mechanism for the spread of disease is well recognized. This study conducted household surveys and measured several physical, chemical, and microbial water quality indicators in 37 elevated storage tanks constructed of different materials (polyethylene, fiberglass, cement) located in a peri-urban community near Cochabamba, Bolivia. Results show that although there is no significant difference in physical and chemical water quality between polyethylene, fiberglass and cement water storage tanks there is a difference in microbial contamination as measured by

E. Coli counts (p = 0.082). Evidence points toward elevated water temperatures that increase along the …

An Evaluation Of The Prevent-Teach-Reinforce (Ptr) Model In A Community Preschool Setting, Laura L. Kulikowski

USF Tampa Graduate Theses and Dissertations

This study evaluated the use of the Prevent-Teach-Reinforce (PTR) model with two four-year-old children in a community preschool classroom. A multiple baseline design across activities was used to assess the teacher implementation of the behavior intervention strategies developed during the PTR team process and the changes in child problem behavior and engagement. Additional measures associated with the outcomes, such as researcher procedural integrity and social validity were assessed. The results suggest that the team of teachers were able to implement the PTR intervention with fidelity, which resulted in a decrease in the children’s problem behavior and an increase in their …

The Relationship Between Knowledge And Beliefs About Human Papillomavirus, Acceptance Of The Human Papillomavirus Vaccine, And Intentions To Practice Safer Sex Behaviors Among Female College Students, Theresa Scorcia-Wilson

USF Tampa Graduate Theses and Dissertations

Introduction. Sexually transmitted infections (STIs) continue to be highly prevalent among young women, and STIs continue to be a challenging health issue on college campuses. Studies have shown that the highest prevalence of human papillomavirus (HPV) is among young adult women, ages 20 to 24, including female college students. While the HPV vaccine has proven to be highly effective in preventing certain high-risk types of HPV, it is not effective in preventing all types of HPV or other STIs. Practicing other safer sex behaviors, in addition to condom use, also can help individuals protect themselves and their partners from …

A Stella Model For Integrated Algal Biofuel Production And Wastewater Treatment, Ivy Cormier

USF Tampa Graduate Theses and Dissertations

Based on a municipal wastewater treatment plant (WWTP) in Tampa, FL, a dynamic multiple-systems model was developed on the STELLA software platform to explore algae biomass production in wastewater by incorporating two photobioreactors into the WWTP‟s treatment train. Using a mass balance approach, the model examined the synergy through algal growth and substrate removal kinetics, as well as macroeconomic-level analyses of algal biomass conversion to biodiesel, biogas, or fertilizer. A sensitivity analysis showed that biomass production is highly dependent on Monod variables and harvesting regime, and profitability was sensitive to processing costs, market prices of products, and energy environment. The …

A Contingent Valuation Of Tampa’S Urban Forest Resource, Alec Foster

USF Tampa Graduate Theses and Dissertations

Urban forests provide environmental, social, and economic benefits to urban residents. These benefits are often overlooked when making spatial and financial distributive decisions in urban areas. The City of Tampa has demonstrated interest in its urban forest resource and estimated its extent and some of the benefits provided. Estimating economic values for benefits that have not been quantified can help to ensure that resources are distributed more efficiently. Five methods to estimate urban forest benefits in the City of Tampa are reviewed, with contingent valuation being the method chosen out of this review process. A mailed, dichotomous choice contingent valuation …

Predictors Of Peer Referral Intentions For Individuals At Risk For Suicide Related Behavior: An Application Of The Theory Of Planned Behavior, Sarah J. Tarquini

USF Tampa Graduate Theses and Dissertations

The role of peer gatekeepers is crucial in connecting individuals at risk for suicide

related behaviors to mental health service providers. However, limited research has

focused on the role of peers as potential helpers for those at-risk. The current study

utilized a mixed experimental and correlational design to examine predictors of female

college students’ referral intentions following hypothetical interactions with peers at-risk

for suicide related behavior. More specifically, the current project examined the utility of

an extended Theory of Planned Behavior (TPB) model of peer-referral intentions. In

addition to the original TPB constructs of attitudes, subjective norms, and perceived

behavioral …

Molecular Mechanism Of Agc Kinases In Human Malignant, Shaokun Shu

USF Tampa Graduate Theses and Dissertations

The maintenance of normal cell function and tissue homeostasis is dependent on the precise regulation of multiple signaling pathways that control cellular decisions to either proliferate, differentiate, arrest cell growth, or initiate programmed cell death (apoptosis). Cancer arises when clones of mutated cells escape this balance and proliferate inappropriately without compensatory apoptosis. Deregulated cell growth occurs as a result of perturbed signal transduction that modulates or alters cellular behavior or function to keep the critical balance between the rate of cell-cycle progression (cell division) and cell growth (cell mass) on one hand, and programmed cell death (apoptosis, autophagy) on the …

Molecular Detection Of The Toxic Marine Diatom Pseudo-Nitzschia Multiseries, Jennifer A. Delaney

USF Tampa Graduate Theses and Dissertations

The marine diatom genus Pseudo-nitzschia includes species that produce domoic acid, a neurotoxin responsible for illness and mortality in both humans and marine wildlife. Because of the expertise and time required for the microscopic discrimination of species, molecular methods that monitor environmental concentrations of Pseudo-nitzschia provide a rapid alternative for the early detection of blooms and prediction of toxin accumulation. We have developed a nucleic acid sequence-based amplification with internal control RNA (IC-NASBA) assay and a quantitative reverse transcription PCR (qRT-PCR) assay for the detection of the toxic species P. multiseries targeting the ribulose- 1,5-biphosphate carboxylase/oxygenase small subunit (rbcS) gene. …

Kinematic And Experimental Evaluation Of Commercial Wheelchair-Mounted Robotic Arms, John William Capille Jr.

USF Tampa Graduate Theses and Dissertations

Commercially available wheelchair-mounted robotic arms (WMRAs) are becoming more prevalent internationally but have yet to be largely developed and approved by the Food and Drug Administration in the United States. The purpose of this study was to experimentally evaluate commercially available WMRAs in a controlled test environment. The goal was to quantitatively compare each device through a standardized testing protocol. The study produced theoretical manipulability measurements as well as efficacy ratings of each device based on Denavit-Hartenberg kinematic parameters and physical testing, respectively. Both the manipulator and control devices of WMRA systems were evaluated. The iARM WMRA system was presented …

Body Depilation Among Women And Men: The Association Of Body Hair Reduction Or Removal With Body Satisfaction, Appearance Comparison, Body Image Disturbance, And Body Dysmorphic Disorder Symptomatology, Michael Scott Boroughs

USF Tampa Graduate Theses and Dissertations

Body depilation, or the reduction or removal of body hair, is a relatively new area of research inquiry. Although women in many industrialized cultures have engaged in depilation for several decades, this behavior has been documented only recently among men. Though originally thought to be widely practiced by women and only a small proportion of men, including athletes or bodybuilders, recent studies suggest that more men engage in body depilation than previously hypothesized. In fact, one recent study estimated the prevalence of men's body depilation at 83.7% which suggests that men are depilating at rates similar to women. Nevertheless sparse …

Ocbs Gone Bad: The Moderating Roles Of Burnout And Role Overload, Kevin Loo

USF Tampa Graduate Theses and Dissertations

Previous literature has typically assumed that organizational citizenship behaviors (OCBs) are beneficial to both employees and organizations. Researchers have begun to question this assumption. This paper seeks to identify situations when OCBs are detrimental to employees or organizations. Specifically, two variables (burnout and role overload) are hypothesized to moderate the relationship between OCBs and outcomes (job satisfaction, turnover intentions, and task performance), such that when burnout and role overload are high, negative outcomes occur. Moderated regression was used to test the hypotheses. There was little evidence for burnout as a moderator, but interactions involving role overload were significant; however, the …

Small Antennas Design For 2.4 Ghz Applications, Ibrahim Turki Nassar

USF Tampa Graduate Theses and Dissertations

In many wireless devices, antennas occupy the majority of the overall size. As compact device sizes become a greater focus in industry, the demand for small antennas escalates. In this thesis, detailed investigations on the design of a planar meandered line antenna with truncated ground plane and 3D dipole antenna at 2.4 GHz (ISM band) are presented. The primary goal of this research is to develop small, low coast, and low profile antennas for wireless sensor applications. The planar meandered line antenna was designed based on a study of different miniaturization techniques and a study of the ground plane effect. …

The Human In 3d: Advanced Morphometric Analysis Of High-Resolution Anatomically Accurate Computed Models, Summer J. Decker

USF Tampa Graduate Theses and Dissertations

Computed virtual models of anatomical structures are proving to be of increasing value in clinical medicine, education and research. With a variety of fields focused on craniofacial and pelvic anatomy there is a need for accurate anatomical models. Recent technological advancements in computer and medical imaging technologies have provided the tools necessary to develop three-dimensional (3D) functional models of human anatomy for use in medicine (surgical planning and education), forensics and engineering (computer-aided design (CAD) and finite element analysis).

Traditionally caliper methodologies are used in the quantitative analysis of human anatomy. In order for experts in anatomy and morphometrics to …

The Biogeochemistry Of Submerged Coastal Karst Features In West Central Florida, Keith Michael Garman

USF Tampa Graduate Theses and Dissertations

West Central Florida is a complex karst environment with numerous sinkholes, springs, and submerged cave systems. Many of these karst features are anchialine, located within the subterranean estuary where freshwater and saltwater mix. Water quality data and/or fauna data were obtained from twenty-one karst features and their associated cave systems. The anchialine karst environment of the study area has a wide range of habitats with measured salinities ranging from freshwater at <0.2 ppt to sulfidic, hypersaline water at 38.5 ppt and measured pH readings ranging from 6.39 in water impacted by sulfur oxidizing bacteria to 10.3 in an isolated room of a cave. Stygobitic crustaceans were identified in conduits extending beneath the Gulf of Mexico supporting the hypotheses that freshwater crustaceans could survive higher sea levels in freshwater conduits beneath saltwater. The fauna associated with the anchialine cave systems included Sabellidae and Polychaeta worms, hydroids, cnidarians and hydrobiid snails. Jewfish Sink, like other anaerobic marine basins that were submarine springs, has four zones: oxic zone, transition zone, upper anoxic zone and anoxic bottom water. The upper zones have seasonal water quality variations from winter cooling and sinking of surface water and changes in the microbial communities. Activity of sulfate reducing bacteria is carbon limited in the anoxic zones, where sulfate reduction is the major metabolic process, and primary production is phosphate limited in the oxic zones. Organic input from the Gulf of Mexico drives the bacterial anaerobic ecosystem, resulting in a “sulfide pump”, in which sulfide percolates upward removing oxygen from the overlying sediment.