Digital Commons Network^™

Cancer And Infection, Kathleen Hope Plummer

USF Tampa Graduate Theses and Dissertations

E. coli is the most frequently isolated Gram negative pathogen from bacteremia in cancer patients and is repeatedly recovered from many other extraintestinal illnesses. These infections are commonly endogenous in nature and interfere with the treatment of cancer resulting in increased healthcare costs, morbidity, and mortality rates. Cancer and the treatments related to cancer cause alterations in the microbiome of the gut and other organs. Despite this point, there is a serious lack of knowledge about the genetic types of E. coli infecting cancer patients. This gap results in vague prevention strategies and limited treatment options for cancer patients. Multi …

Manipulating The Tumor Microenvironment For Therapeutic Benefit, Kate M. Bailey

USF Tampa Graduate Theses and Dissertations

The physical tumor microenvironment contributes significantly to carcinogenesis, cancer progression and metastatic dissemination. Two main components of the tumor microenvironment, hypoxia and acidosis, are present in nearly every solid tumor and act as powerful selection forces against the tumor. Hypoxia and acidosis promote tumor heterogeneity and contribute to chemotherapy and radiotherapy resistance. This dissertation interrogates methods to target the tumor microenvironment including two novel studies describing mechanisms of buffer therapy resistance and targeting tumor hypoxia with vasodilators to enhance the efficacy of a hypoxia activated prodrug, TH-302.

In the first study, mechanisms of buffer therapy resistance were identified and detailed. …

Rna Detection Technology For Applications In Marine Science: Microbes To Fish, Robert Michael Ulrich

USF Tampa Graduate Theses and Dissertations

The accurate identification of taxa from mixed assemblages using genetic analysis remains an important field of molecular biology research. The common principle behind the development of numerous documented genetic detection technologies is to exploit specific nucleotide sequences inherent to each taxon. This body of work focuses on practical applications of real-time nucleic acid sequence-based amplification (RT-NASBA) in marine science, and is presented in four case studies. Each study represents novel work in the genetic identification of respective taxa of interest using RT-NASBA. Two case studies documented the development of an assay targeting mitochondrial 16S rRNA to discern legally salable grouper …

Applying Modeled Hemi-Ellipsoids To The Study Of Pressure Distribution In Normal And Paraplegic Seated Subjects, Alicia Billington

USF Tampa Graduate Theses and Dissertations

The three goals of this research were to investigate how normal subjects move while seated, how paraplegic patients move while seated, and whether seated movements can be modeled using a hemi-ellipsoid shape. Pressure readings were recorded at 11 Hz using a 36 by 36 sensor pressure map by XSENSOR. Subjects were instructed to move or perform pressure relief as they normally would while seated. Analysis was performed using Microsoft Excel with Solver and Matrix.xla add-ins and automated with VBA code. Major movements and time intervals between movements were calculated by locating the area of maximum pressure on each hemi-buttock for …

Topological Data Analysis Of Properties Of Four-Regular Rigid Vertex Graphs, Grant Mcneil Conine

USF Tampa Graduate Theses and Dissertations

Homologous DNA recombination and rearrangement has been modeled with a class of four-regular rigid vertex graphs called assembly graphs which can also be represented by double occurrence words. Various invariants have been suggested for these graphs, some based on the structure of the graphs, and some biologically motivated.

In this thesis we use a novel method of data analysis based on a technique known as partial-clustering analysis and an algorithm known as Mapper to examine the relationships between these invariants. We introduce some of the basic machinery of topological data analysis, including the construction of simplicial complexes on a data …

Can The Usa National Weather Service Heat Index Substitute For Wet Bulb Globe Temperature For Heat Stress Exposure Assessment?, Ivory Iheanacho

USF Tampa Graduate Theses and Dissertations

Heat stress occurs when the body cannot adequately cool itself due to the combined contributions of metabolic heat, environmental factors and clothing. Heat stress found in the workplace puts employees at risk of developing heat-related illnesses, disorders and could be fatal. The wet bulb globe temperature (WBGT) index is the current method used to assess environmental contributions to heat stress in an occupational setting. The purpose of this thesis was to explore whether the National Weather Service's Heat Index (HI) could substitute for the WBGT Index during occupational heat stress exposure assessment. The possibility of using the HI for heat …

Low-Cost Household Groundwater Supply Systems For Developing Communities, Michael Maccarthy

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Self-supply is widely reported across various contexts, filling gaps left by other forms of water supply provision. This research assesses low-cost household groundwater supply technologies in markets in developing country contexts of sub-Saharan Africa and Latin America, with a focus on the potential for improving Self-supply technology implementation and markets in sub-Saharan Africa. Specifically, a mature and unsubsidized Self-supply market for Pitcher Pump systems (suction pumps fitted onto hand-driven boreholes) is studied in an urban context in Madagascar, EMAS low-cost water supply technologies are assessed in Bolivia, and a technical comparison is completed with manual EMAS Pumps and family …

Environment, Rights, And Waste In Bolivia: Addressing Water And Sanitation Processes For Improved Infrastructure, Maryann R. Cairns

USF Tampa Graduate Theses and Dissertations

Water and sanitation (WatSan) development projects impact both natural systems and societal structures where they are placed. A complex process of development, including inter-governmental policies, aid agencies, personal relationships, and community politics enhance and constrain the efficacy of these projects. This study presents the many ways in which the WatSan development process has unintended and unexpected returns for certain community groups. Using a political ecology framework, I look at power structures, perceived and projected environmental impacts, multiple stakeholders, and individual discourses to critique how the right to water and sanitation is implemented in a specific community context. This project advances …

The Relationship Of Affective Domains And Cognitive Performance In Paramedic Students, Katie J. Lyman

USF Tampa Graduate Theses and Dissertations

No universal approach for application procedures has been established for paramedic curriculum programs. The field of pre-hospital, Emergency Medical Services (EMS) has evolved quickly from its inception to present date. The educational components of EMS are still in their infancy and lack evidence-based protocols (Drees, 2006). Predicting success in an allied health program typically concentrates on some type of academic instrument. The use of personality inventories has been underexplored; however, literature reveals they may be more reliable in determining academic and employment success compared with other non-cognitive tools (Groves, Gordon, & Ryan, 2007; Marrin et al., 2004; McManus & Richards, …

Negative Reinforcement In Infant Care Simulation: Alternative Caregiver Responses To Prevent Child Abuse, Miriam Tye

USF Tampa Graduate Theses and Dissertations

This study was conducted to replicate and extend previous research on infant caregiver behavior by demonstrating negative reinforcement of infant caregiver behavior in response to crying and teaching appropriate care responses under conditions of inconsolable crying. A computerized infant simulator was used to create a laboratory simulation of infant caregiving. In Study 1, participants were exposed to negative reinforcement conditions and an extinction condition. In the negative reinforcement condition, participants engaged in caregiving responses to escape from the cry. In the extinction condition, the cry was inescapable and two of three participants stopped engaging in the previously reinforced caregiving response. …

The Use And Effectiveness Of Online Social Media In Volunteer Organizations, Amy J. Connolly

USF Tampa Graduate Theses and Dissertations

Volunteer organizations face two challenges not found in non-volunteer organizations: recruiting and retaining volunteers. While social media use is increasing amongst individuals, its use and effectiveness for volunteer recruitment and retention by volunteer organizations is unknown. The dissertation reports the results of three studies to investigate this important question. Using a mixed-methods approach, it addressed the dual nature of social media and its effectiveness by including volunteer organizations and social media users.

This dissertation found that although volunteer organizations are not using social media effectively, they could virtualize requirements of the recruitment process by focusing on relatable events instead of …

Characterization Of Waste Anesthetic Gas Exposures To Veterinary Workers In The Tampa Bay Area, Kyle Vogel

USF Tampa Graduate Theses and Dissertations

Veterinarians routinely conduct surgical operations on animals while veterinary technicians administer anesthetic gas to sedate the animal prior to an operation. One commonly used anesthetic agent in veterinary clinics is isoflurane. Veterinary workers have the potential to be exposed to this gas during surgical operations. The Occupational Safety and Health Administration (OSHA) does not have a specific Permissible Exposure Limit (PEL) for isoflurane, however The National Institutes for Health (NIH) does advise that workers should not be exposed to isoflurane in concentrations exceeding 2 parts per million (ppm) for an 8 hour Time Weighted Average (TWA).

Animal clinics vary in …

Crisis Communication: Sensemaking And Decision-Making By The Cdc Under Conditions Of Uncertainty And Ambiguity During The 2009-2010 H1n1 Pandemic, Barbara Bennington

USF Tampa Graduate Theses and Dissertations

Abstract

This study focuses on the process of communication between government agencies and the public during crisis situations, and the development of an effective response strategy when a significant threat to public health and/or safety is believed to exist. My specific research interests are (1) the nature of the decision-making process that influences the communicative choices made during such events, and (2) how decision-makers make sense of an evolving, ambiguous, and unpredictable situation, in order to establish credibility with the public, determine the appropriate response strategy, and gain the public's trust in order to influence its behavior. This is a …

The Use Of Behavior Specific Praise And The Caught Being Good Game To Improve Class-Wide Behavior, Emily Rhodes

USF Tampa Graduate Theses and Dissertations

This study evaluated the relative contributions of behavior specific praise statements (BSPS) and the Caught Being Good Game (CBGG) on class-wide appropriate behaviors and examined teacher use of BSPS and corrective feedback. It also evaluated if changes in class-wide behaviors are maintained during follow-up and generalized to non-target academic periods. Data on teacher use of BSPS were also collected during follow-up and generalization probes to examine if the teachers continued to use BSPS during follow-up and generalize their use of BSPS to non-target academic periods. A multiple-baseline design across classrooms with an ABC sequence was used to evaluate the outcomes …

Development And Assessment Of Self-Explaining Skills In College Chemistry Instruction, Adrian Villalta-Cerdas

USF Tampa Graduate Theses and Dissertations

The prevalent trend in chemistry instruction relies on what has been described as the classroom game. In this model, students take a passive role and the instructor does all the explaining (thinking), and learning is trivialized to knowing the correct answers (memorizing) and being able to produce them when prompted (regurgitating). The generation of explanations is central to scientific and technological development. In the process of figuring out explanations, the generation of inferences relies on the application of skills associated with scientific behaviors (e.g., analytical reasoning and critical thinking). The process of explanation generation causes a deeper analysis and revision …

Novel Cell Cycle Proteins In Apicomplexan Parasites, Carrie Butler

USF Tampa Graduate Theses and Dissertations

Apicomplexans are responsible for major human diseases such as toxoplasmosis caused by Toxoplasma gondii (T. gondii) and the deadliest form of malaria caused by Plasmodium falciparum (P. falciparum). The genomes of these pathogens are now sequenced ushering in a new era of drug development. A major hurdle to exploiting this genome resource is that a large number of the encoded genes are "hypotheticals" and have yet to be characterized. Hypothetical proteins comprise roughly half of the predicted gene complement of T. gondii and P. falciparum and represent the largest class of uniquely functioning proteins in these parasites.

Following the idea …

Diffuse Nutrient Pollution From Residential Catchments, Melissa Rachelle Butcher

USF Tampa Graduate Theses and Dissertations

Nonpoint source nutrient pollution is diffuse pollution lacking discrete origin and conveyance. This thesis synthesizes and critically reviews research on residential nitrogen and phosphorus loss to stormwater runoff and leaching. The evaluation pulls from research covering influential socio-demographic indicators, such as use of lawn maintenance services and homeowner fertilizer practices. The extent to which such social and economic factors may influence the prevalence and fate of diffuse nutrients in stormwater runoff from residential areas has not been adequately established. Understanding the source and influencing factors of diffuse nutrient pollution is important in order to effectively protect surface and groundwater resources. …

Inactivation Of Ascaris In Double-Vault Urine-Diverting Composting Latrines In Panama: Methods And Environmental Health Engineering Field Applications, Daragh A. Gibson

USF Tampa Graduate Theses and Dissertations

The United Nations Millennium Development Goals have prioritized improving access to sanitation, but unfortunately about a third of the global population is still without an improved sanitation source and one billion still practice open defecation. Lack of access to adequate and safe sanitation means the proliferation of dangerous pathogens in the environment, especially soil-transmitted helminths (STHs). In the Bocas del Toro Province of Panama (and similar locations in the world), composting latrines have been built in many of the indigenous communities in the area. They are a form of dry or ecological sanitation and are designed to produce an end …

Age-Associated Increases In Fkbp51 Facilitate Tau Neurotoxicity, Laura J. Blair

USF Tampa Graduate Theses and Dissertations

Tau is a protein which regulates microtubule stability and is heavily involved in axonal transport. This stability is dynamically controlled in part by over 40 phosphorylation sites across the tau protein which allows for binding and release from the microtubules. However, if abnormal hyperphosphorylation occurs, tau dissociates from the microtubules. Once released, the microtubules become unstable and the aberrant tau mislocalizes from the axon to the somatodendric compartment, where it aggregates. These aggregates are made of many pathological forms of tau including oligomeric species, paired helical filaments, and neurofibrillary tangles, all of which have associated toxicities. Tau pathology is a …

Improving Consistency Of Goal Attainment To Increase Physical Activity, Elizabeth Anne Solley

USF Tampa Graduate Theses and Dissertations

Researchers have successfully increased physical activity with self-monitoring, goal setting, and feedback. Goal attainment is a crucial part of what makes goal setting successful; however, it is often unreported in the literature or implied that goals were not reached consistently. A potential way to achieve this consistency is to create an action plan, or a detailed account of exactly how and when the individual will engage in the desired physical activity to reach his or her goal. This study evaluated whether making a detailed action plan would allow individuals to reach their physical activity goals more consistently than when using …

Using Video Feedback To Increase Eye Contact During Mock Job Interviews For Transition Age Adults With Autism Spectrum Disorders, Alexia Barnes

USF Tampa Graduate Theses and Dissertations

Abstract

Autism Spectrum Disorders (ASD) are characterized by impairments in social and communicative behaviors. These impairments can impact an individual's ability to obtain employment. The rates of unemployment for individuals with ASD are much higher than those of their peers without ASD. This study used a multiple baseline design across three participants diagnosed with ASD to determine the effectiveness of video feedback in improving eye contact duration during mock job interviews. After video feedback sessions, eye contact duration increased across all participants. On average, participant's eye contact duration was at 30.2% during baseline. These percentages increased to an average of …

Water And Health In The Nandamojo Watershed Of Costa Rica: Community Perceptions Towards Water, Sanitation, And The Environment, James Mcknight

USF Tampa Graduate Theses and Dissertations

Understanding the relationships between human health, water, sanitation, and environmental health is a requirement to understanding the challenges that face researchers when it comes to addressing global health relating to water and sanitation. Access to improved water and sanitation is not only a precondition to health, but to all aspects of daily living. Target 7.C of the Millennium Development Goals (MDGs) addresses worldwide disparities in access to improved water and sanitation by calling for the reduction in "half of the proportion of people without sustainable access to safe drinking water and sanitation by 2015". Over 90% of the population of …

Evaluating The Efficacy Of Shaping With A Percentile Schedule To Increase The Duration Of Sustained Interaction Following A Bid For Joint Attention In Children With Autism, Therese Gutbrod

USF Tampa Graduate Theses and Dissertations

This study examined the use of shaping with a percentile schedule to increase the duration of the interaction following a bid for joint attention in children with autism. Specifically, the therapist initiated a bid for joint attention and reinforced longer successive approximations in seconds of sustained interaction with the therapist and activity. A percentile schedule ranked the most recent 10 observations and reinforcement was provided if the current observation equaled the sixth ranking. Most-to-least prompting was used if the child failed to meet the calculated criterion. Shaping with a percentile schedule of reinforcement was effective at increasing the duration of …

Targeting Cancer Metabolism With Ketosis And Hyperbaric Oxygen, Angela M. Poff

USF Tampa Graduate Theses and Dissertations

Cancer cells exhibit an abnormal metabolic phenotype characterized by glycolysis and lactate fermentation in the presence of oxygen, a phenomenon known as the Warburg effect. This dysregulated metabolism plays an important role in every aspect of cancer progression, from tumorigenesis to invasion and metastasis. The Warburg effect is a common phenotype shared by most, if not all, cancer types. It is especially prominent in metastatic tumors, which are notoriously resistant to treatment and responsible for the majority of cancer-related deaths. Thus, metabolic therapies which target the Warburg effect could offer novel therapeutic options for most cancer patients, including those with …

Using Otolith Elemental Composition To Track The Habitat Use, Movements, And Life History Patterns Of Common Snook (Centropomus Undecimalis) And Red Drum (Sciaenops Ocellatus) In The Tampa Bay Estuary, Holly Jacqueline Rolls

USF Tampa Graduate Theses and Dissertations

Knowledge of fish habitat use and connectivity is critical for understanding the structure and dynamics of fish populations and, therefore, necessary for the implementation of successful fisheries management strategies. Tagging is an effective means of providing such information, and the elemental composition contained within fish otoliths is increasingly being used as a natural tag. The chemical composition of otoliths reflects the incorporation of elements from different water bodies and can thus be used to understand the habitat use, movements, and life history patterns of fishes. To assess the applicability of otolith elemental composition as a tagging technique within the Tampa …

Rtemis: Real-Time Tumoroid And Environment Monitoring Using Impedance Spectroscopy And Ph Sensing, Frank Alexander

USF Tampa Graduate Theses and Dissertations

This research utilizes Electrical Impedance Spectroscopy, a technique classically used for electrochemical analysis and material characterization, as the basis for a non-destructive, label-free assay platform for three dimensional (3D) cellular spheroids. In this work, a linear array of microelectrodes is optimized to rapidly respond to changes located within a 3D multicellular model. In addition, this technique is coupled with an on chip micro-pH sensor for monitoring the environment around the cells. Finally, the responses of both impedance and pH are correlated with physical changes within the cellular model. The impedance analysis system realized through this work provides a foundation for …

The Rhetoric Of Corporate Identity: Corporate Social Responsibility, Creating Shared Value, And Globalization, Carolyn Day

USF Tampa Graduate Theses and Dissertations

In today's global political and media climate, the stakes are high for corporations, local or otherwise, to create and maintain an `ethical' perception of not only their daily business activities and how they can benefit society or protect the environment, but also their enduring characteristics or `corporate identity' (Conrad, 2011) for numerous, sometimes conflicting stakeholder audiences (Cheney, 1983). This dissertation examines how such forms of `socially responsible' corporate identities are created and maintained through the use of persuasive language. In particular it examines the role and implications of rhetoric within the contexts of Corporate Social Responsibility (CSR), as well as …

The Fight Within: Experiences Of School District Employees Who Advocate For The Rights Of Their Own Children With Disabilities Inside The Districts Where They Work, A Heuristic Case Study, Keri Haley

USF Tampa Graduate Theses and Dissertations

Parents of children with disabilities face a daunting task when it comes to advocating for the rights of their children in school districts across the country. Yet, when these same parents also work inside those school districts, the challenge to balance their advocacy within the expectations of their employment may come with barriers. The intent of this study was to understand the experiences of people who are parents of children with disabilities, as well as educators, and have had to advocate for their own children inside the school districts where they work. Using a heuristic case study approach, this study …

Trend Analysis And Modeling Of Health And Environmental Data: Joinpoint And Functional Approach, Ram C. Kafle

USF Tampa Graduate Theses and Dissertations

The present study is divided into two parts: the first is on developing the statistical analysis and modeling of mortality (or incidence) trends using Bayesian joinpoint regression and the second is on fitting differential equations from time series data to derive the rate of change of carbon dioxide in the atmosphere.

Joinpoint regression model identifies significant changes in the trends of the incidence, mortality, and survival of a specific disease in a given population. Bayesian approach of joinpoint regression is widely used in modeling statistical data to identify the points in the trend where the significant changes occur. The purpose …

The Reproductive Lives Of Chuukese Women: Transnationalism In Guam And Chuuk, Sarah Ann Smith

USF Tampa Graduate Theses and Dissertations

Chuuk, one state of the Federated States of Micronesia (FSM), experiences significant transnational migration to the United States (U.S.), particularly to the Territory of Guam. This migration is facilitated by the Compact of Free Association (COFA), an agreement with several Micronesian countries previously under U.S. administration that allows for free movement of their citizens into the U.S. Although part of Micronesia, Guam's colonized residents resist an identity connected to rest of Micronesia. With very poor health outcomes, the Chuukese represent a political and social body of bodies that bring sickness, babies and increased costs to the Guam government without adequate …