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Receptor-Associated Protein (Rap) Models In Vivo Reelin Haploinsufficiency: Implications In Schizophrenia, Jamileh Ahmed

USF Tampa Graduate Theses and Dissertations

The “two-hit” schizophrenia hypothesis suggests genetic and environmental abnormalities interrupt early CNS function. This increases vulnerability of a “second hit” and schizophrenia onset. Chronic stress and decreased Reelin signaling are reportedly associated with schizophrenia. Heterozygous Reeler Mice (HRM) show a 50% reduction in Reelin and display major schizophrenia phenotypes. Receptor-Associated Protein (RAP) blocks ligand-association to Reelin receptor Apolipoprotein E receptor 2 (ApoER2). In this study, we sought to replicate major heterozygous reeler mouse (HRM) phenotypes using in vivo RAP studies to establish an experimental in vitro model. Using an in vitro model, we investigated the effects of chronic stress and …

Promoting Happiness In Elementary Schoolchildren: Evaluation Of A Multitarget, Multicomponent Classwide Positive Psychology Intervention, Brittany Valle Hearon

USF Tampa Graduate Theses and Dissertations

Youth psychological well-being has become increasingly acknowledged as not merely the absence of psychological distress, but the presence of positive indicators of optimal functioning. Students with complete mental health (i.e., low psychopathology and high well-being) demonstrate the best academic, social, and physical health outcomes. As such, there remains a need to address children’s well-being through a holistic approach emphasizing the prevention of mental health problems and promotion of flourishing. Positive psychology interventions (PPIs) have emerged as a promising method of enhancing students’ complete mental health. Previous investigations support the utility of multitarget PPIs with middle school students and single-target PPIs …

Healthcare Costs Of Injured Youth: The Need For Prevention, Policy, And Proper Triage, Jessica Lynn Ryan

USF Tampa Graduate Theses and Dissertations

Objective

The goal of this dissertation was to identify evidence regarding potential means to reduce healthcare spending on youth injury while protecting and promoting the health of our youth. The first analysis estimated and analyzed both the financial costs and time lost from sports injuries among inpatient and ED youth patients to aid in identifying key populations, raising awareness to policy makers, and emphasizing the need of prevention programs for sports injury. The second analysis analyzed the effect of volume and trauma center (TC) ownership type on trauma alert response charges, which are billed to injured patients for a trauma …

The Relationship Between Trait Emotional Intelligence And L2 Motivation, Jelena Vuksanovic

USF Tampa Graduate Theses and Dissertations

Motivation is considered an important factor in initiating and sustaining the second language (L2) process. Since learning an L2 can be seen as a lengthy process, one that needs sustained motivation over a long period of time, learners need to be able to successfully deal with their and others’ emotions in order to generate and sustain their motivation for learning. The role of learners’ emotions and trait emotional self-efficacy, or trait emotional intelligence (EI), in L2 motivation and language learning has been a relatively unexplored area. To fill this gap, the current study posed three research questions that addressed the …

Non-Classical Regulators In Staphylococcus Aureus, Andy Weiss

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a highly problematic human pathogen due to its ability to cause devastating infections, paired with a capacity to withstand the action of a large fraction of available antibiotics. Both pathogenicity and antibiotic resistance are encoded by numerous genomic elements, though the expression of these factors is energetically costly and not always beneficial for cellular survival. Therefore, S. aureus has developed sophisticated regulatory networks to integrate a multitude of signals, enabling it to navigate the delicate balance between its pathogenic lifestyle and baseline needs for cellular energy homeostasis. It is thus imperative to study S. aureus behavior and …

Investing In Change: Illuminating Interactive Systems In Hiv Research, Communication Diffusion, And Financing In Lesotho, Sharon Elizabeth Watson

USF Tampa Graduate Theses and Dissertations

In the field of HIV, more than 30 years into the epidemic, the need to ensure that what researchers learn makes its way into tangible actions in the real world is especially poignant. This dissertation addresses the critical divide between research production and its translation into practice. It advances ways to measure the investments of citizens and stakeholders in qualitative studies and offers new perspectives on the losses inadvertently caused by particular investments in health research and services. Unfortunately, many of the problems in how we practice and disseminate research are rampant throughout the health and development research sector. Therefore, …

Learner-Directed Vs. Instructor-Provided Curriculum Among Undergraduate Students, Christopher D. Martinez

USF Tampa Graduate Theses and Dissertations

There has been little or no research on the use of a Learning Object in the field of religious studies. The purpose of this research study was to assess undergraduate student knowledge gain outcomes between instructor-provided and learner-directed methods, which is the independent variable, using asynchronous, online, narrated PowerPoint presentations compared to undergraduate student knowledge gain using an online learning-objects unit, in a college-level world religions survey course at St. Petersburg College. This study assessed undergraduate student knowledge gain outcomes between instructor-provided and learner-directed methods among 90 college undergraduate students and determined whether there was a significant difference in the …

The Influence Of Tropical Forests And Climate Change On The Fates Of Select Organic Pollutants In A Jamaican Watershed, Kayon Barrett

USF Tampa Graduate Theses and Dissertations

Many organic compounds, although beneficial, are associated with negative health and ecological impacts. It is therefore imperative to understand the environmental fates of these contaminants. Whereas the fates and health impacts of many persistent organic pollutants have been extensively examined, there is limited research characterizing the fates of these and the less persistent organic compounds in tropical multi-use watersheds. This study therefore aimed to evaluate the roles of forests and climate change on the environmental fates and health impacts of select organic chemicals in the Rio Cobre watershed, a tropical river basin in Jamaica. A total of 16 organic compounds …

Acetaminophen Associated Neurotoxicity And Its Relevance To Neurodevelopmental Disorders, Seol-Hee Kim

USF Tampa Graduate Theses and Dissertations

Autism is a lifelong neurodevelopmental disorder. The etiology of autism still remains unclear due to the heterogeneous and complex nature of the disorder, however synergistic actions between genetic components and environmental factors have been suggested. Acetaminophen (APAP) is one of the most popular over-the-counter drugs that possess antipyretic and analgesic effects. It is considered a relatively safe and effective within therapeutic doses. Recently, early exposure to APAP has been suggested to be one of the underlying cause of autism. Children are often prescribed APAP to lessen fever or irritability after vaccination during the first year, and APAP may adversely affect …

Multi-Scale Approaches For Evaluating The Success Of Habitat Restoration In Tampa Bay, Florida, Stephanie Thompson Powers

USF Tampa Graduate Theses and Dissertations

This research aims to further the understanding of ecological restoration success in the Tampa Bay, Florida, region. Although over four hundred restoration projects have been completed in the bay area, knowledge of their success has been hindered by the lack of assessment and transfer of information concerning project outcomes. Without comprehensive project assessment, local science will be limited in its ability to inform practice because we lack the advantage of past knowledge.

Using a multi-scaled approach, a diverse set of restoration projects are evaluated, providing information on how the projects are contributing to defined targets established by the Tampa Bay …

Germania- And Silica-Based Perfluorinated And Non-Fluorinated Sol-Gel Sorbents For Capillary Microextraction In Chromatographic Analysis, Emre Seyyal

USF Tampa Graduate Theses and Dissertations

Sample preparation is the most time-consuming and error-prone step in chemical analysis. Miniaturization and automation of the sample preparation equipment eliminating or reducing the use of hazardous organic solvents, online hyphenation of sample preparation with analytical instruments in a cost-effective way are important factors that need to be considered to design and implement innovative sample preparation techniques and strategies. Solid-phase microextraction (SPME) is a simple, environmentally benign technique well suited for hyphenation with analytical instruments. However, poor coating stability is a significant drawback of SPME employing conventionally prepared coatings. This shortcoming arises from the lack of chemical bonding between the …

Parent Involvement Factors From The Perspectives Of Academically Successful Black Male College Students, Vernon Leo Stanley Smith

USF Tampa Graduate Theses and Dissertations

During the past two decades, concerns about Black males’ academic achievement have been a major theme in the literature devoted to education and social sciences. Despite over two decades of studies focused specifically on Black males and their academic underachievement, the achievement gap persists. According to the National Center for Educational Statistic (2006), African-American males were reported as being disproportionally negatively represented in regards to academic achievement. Some researchers suggested Black children’s underachievement might be due to the lack of parental involvement. Other studies have highlighted statements of Black males who indicated parental involvement factors that contributed to their academic …

Partial Mediation And Moderation Effects Of Class Peer Norms On The Relations Of Teacher Support With Student Aggression And Academic Engagement During Early Adolescence, Huanhuan Wang

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine the mediation and moderation effects of classroom peer norms (CPN) on the associations of teacher support with student engagement and aggression during sixth grade (first year of middle school). Current literature suggests that early adolescence is a critical developmental period with many changes occurring, including a peak in aggression, decreased academic engagement, increased peer influence, and decreased teacher support. Despite these challenging changes, teachers often serve as a powerful yet “invisible hand” (Farmer, Lines, & Hamm, 2011) that can influence student behaviors directly or indirectly via CPN. However, these two routes of …

In Vivo Channel Characterization And Energy Efficiency Optimization And Game Theoretical Approaches In Wbans, Yang Liu

USF Tampa Graduate Theses and Dissertations

This dissertation presents several novel accomplishments in the research area of Wireless Body Area Networks (WBANs), including in vivo channel characterization, optimization and game theoretical approaches for energy efficiency in WBANs.

First, we performed the in vivo path loss simulations with HFSS human body model, built a phenomenological model for the distance and frequency dependent path loss, and also investigated angle dependent path loss of the in vivo wireless channel. Simulation data is produced in the range of 0.4−6 GHz for frequency, a wide range of distance and different angles. Based on the measurements, we produce mathematical models for in …

Perception Of School Staff Regarding The Effectiveness Of A Youth Mental Health Program In Schools, Hiba Chehaib Chehaib

USF Tampa Graduate Theses and Dissertations

There is an ongoing debate about how to serve the number of students that are affected by mental health problems. Some schools have moved toward implementing whole school programs. The purpose of these programs is to teach adults who directly interact with school age children, such as parents, family members, caregivers, teachers, school staff, and caring citizens how to respond to an adolescent that is facing a crisis or a mental health challenge. The purpose of this study is to evaluate the perceived effectiveness of such a program. The goals of this study are to (a) determine the short-term outcomes …

An Exploration Of Self-Compassion And Wellness Behaviors Among Practicing School Counselors, Anjanette Todd

USF Tampa Graduate Theses and Dissertations

According to the American School Counseling Association (ASCA, 2010), school counselors are charged with meeting the academic, social/emotional and career needs of all students (2010). In addition, school counselors serve as the front line in dealing with trauma and crisis in their schools. Those counselors who understand the importance of their own wellness and practice healthy lifestyle behaviors are better equipped to meet these demands.

Unfortunately, not all school counselors are practicing what they advocate for their students relative to promoting wellness in their own lives (O'Halloran & Linton, 2000). This lack of congruence, where school counselors are not modeling …

Severe Maternal Morbidity In Florida: Risk Factors And Determinants Of The Increasing Rate, Lindsay Shively Womack

USF Tampa Graduate Theses and Dissertations

Severe maternal morbidity generally refers to the most severe complications of pregnancy and includes: hemorrhage, embolism, acute renal failure, stroke, acute myocardial infarction, and other complications. These complications affect more than 50,000 women in the United States every year, with rates significantly increasing from 1998 to 2011. In an effort to reduce these increasing complication rates, clinicians and researchers have emphasized the need to identify potential modifiable risk factors for severe maternal morbidity, and the need to study the relationships between these risk factors and severe maternal morbidity. The overall goal of this study is to improve the understanding of …

Physical Therapy Utilization And Length Of Stay Among Patients With Low Back Pain In Florida Hospitals, Kyle A. Watterson

USF Tampa Graduate Theses and Dissertations

Purpose: The purpose of this work was identify key factors associated with inpatient physical therapy utilization and length of stay for patients with low back pain (LBP) in Florida hospitals.

Rationale: Little is known about factors associated with inpatient physical therapy or length of hospitalization for patients with LBP. This group of works identified the key factors associated with inpatient physical therapy and long lengths of hospitalization for this patient population. Since physical therapy and reduced length of stay are known cost-reducers, identifying key factors may represent significant cost savings to the health care system.

Methods: Several mixed method procedures …

Neuropeptide Modulation Of The Large Conductance Potassium (Bk) Channel In The Auditory System: Therapeutic Implications For Age-Related Hearing Loss, Ellliott James Brecht

USF Tampa Graduate Theses and Dissertations

The auditory temporal processing deficits associated with age-dependent hearing decline have been increasingly attributed to issues beyond peripheral hearing loss. Age-related hearing loss (ARHL), also known as presbycusis, is linked with changes in the expression of both excitatory and inhibitory neurotransmitters in the central auditory system. There are also age-related changes in the expression and function of the ion channels which mediate action potential firing. The slow, Ca²⁺ activated, K⁺ channels of the BK-type are essential in controlling both neurotransmitter release and neural communication via alteration of action potential durations, firing frequency, and neural adaptation. There are many …

Many Hands Make Light Work: Crowdsourced Ratings Of Medical Student Osce Performance, Mark Grichanik

USF Tampa Graduate Theses and Dissertations

Clinical skills are often measured using objective structured clinical examinations (OSCEs) in healthcare professions education programs. As with assessment centers, it is challenging to provide learners with effective feedback due to burdensome human capital demands. The aim of this dissertation was to evaluate the viability of using a crowdsourced system to gather OSCE ratings and feedback. Aggregating evaluations of student performance from a crowd of patient proxies has the potential to mitigate biases associated with single-rater evaluations, allow the patient a voice as the consumer of physician behavior, improve reliability, reduce costs, improve feedback latency, and help learners develop a …

The Sustainability Of Career And Technical Education Teacher Preparation Programs In Florida, Lisa Martino

USF Tampa Graduate Theses and Dissertations

There is a historical trend of decline in available Career and Technical Education (CTE) Teacher Preparation programs and an increase in CTE course and program enrollments in Florida’s secondary school system. This contradiction in CTE teacher supply and demand raises an important question. What is going on? More specifically, what sustains the remaining programs in the face of this decline?

The purpose of this qualitative study is to explore sustainability factors from past and present CTE teacher preparation program educators and administrators to gain a better understanding of this issue. The participant selection for this study was identified from four …

Institutional Review Boards And Writing Studies Research: A Justice-Oriented Study, Johanna Phelps-Hillen

USF Tampa Graduate Theses and Dissertations

In this multi-method dissertation project I conduct policy analysis and utilize results from a discipline-wide survey (n=258) to examine the intersection of Writing Studies researchers’ disciplinary affiliation, research context, and personal disposition in relation to the local implementation of federal policy regarding human subjects research. I elaborate on the context of this project, discussing the September 2015 release of the Notice of Proposed Rulemaking (NPRM) to revise and update the Common Rule, 45.CFR Part 46, and the Conference on College Composition and Communication’s formal comment in response to the proposed rule’s provisions. I discuss the process of designing and implementing …

Acute Kidney Injury And Chronic Kidney Disease, Jin Wei

USF Tampa Graduate Theses and Dissertations

Ischemia and reperfusion are natural steps during kidney transplantation, and IRI is considered one of the most important nonspecific factors affecting allograft dysfunction. Transplanted organs experience several episodes of ischemia, in which cold ischemia occurs during allograft storage in preservation solutions.

Even though cold ischemia has been studied extensively, all of the studies have been carried out in vitro and ex vivo models. There is no in vivo model available to examine renal IRI induced solely by cold ischemia.

In the present study, we developed an in vivo mouse model to study renal IRI induced exclusively by cold ischemia through …

The Sustainability Of Ion Exchange Water Treatment Technology, Adib Amini

USF Tampa Graduate Theses and Dissertations

This research investigated using a life cycle environmental and economic approach to evaluate IX technology for small potable water systems, allowing for the identification and development of process and design improvements that reduce environmental impacts and costs. The main goals were to evaluate conventional IX in terms of life cycle environmental and economic sustainability, develop a method for improving designs of IX systems from a environmental and economic sustainability standpoint, evaluate potential design improvements, and make the research findings accessible to water professionals through user-friendly tools and frameworks that take into account their feedback. This research provides an understanding, from …

The Optimization Of The Nuclear Protein In Testis (Nut) Antibody And Its Importance And Impact In The Pathology Lab, Lindsey Martinez

USF Tampa Graduate Theses and Dissertations

Optimization in immunohistochemistry is often a time consuming and complex process. There are a varying array of moving parts to consider all while preserving the sensitivity and specificity of the test. When optimizing an antibody it is important to consider the fixation of the tissue and the type of epitope retrieval that would be best suited for the test. The dilution of the primary antibody is a key marker for the efficiency and effectiveness of the laboratory protocol. The purpose of this study was to produce an optimized antibody for the nuclear protein in testis to detect NUT midline carcinoma …

Poverty In The Land Of Plenty? Deconstructing Role Of Community-Based Organizations In A Small Community, John Kevin Trainor

USF Tampa Graduate Theses and Dissertations

Using the lens of a community-based childhood obesity intervention, it is possible to examine the role of non-profit organizations in community development and to deconstruct the “community” in community-based research and identify the many competing interests within a community. This contextual understanding includes how the community is formed, how a community’s agenda is set, and who will complete the tasks outlined in that agenda. In applied anthropological settings and public health interventions that are community-based, it is essential to understand the context of community and which community (or communities) the researcher is working with to ensure that the data you …

Strategies For Reducing Preventable Hospital Readmissions On Medicare Patients, Andres Patricio Garcia-Arce

USF Tampa Graduate Theses and Dissertations

The high expenditure of healthcare in the United States (U.S.) does not translate into better quality of care. Indeed, the U.S. healthcare system is recognized by its lack of efficiency and waste (which represents about 20% of the country’s healthcare expenses). Lack of coordination is one of the most referenced causes of waste in the U.S. healthcare system, and preventable hospital readmissions have been acknowledged to be evidence of poor coordination of care. In fiscal year 2013, the Centers for Medicare and Medicaid Services (CMS) established financial penalties for inpatient care reimbursements in hospitals with excessive readmissions. All the same, …

The Potential Role Of Antiretroviral Efavirenz In Hiv Associated Neurocognitive Disorders, Lecia Ashanna Moya Brown

USF Tampa Graduate Theses and Dissertations

The prevalence of milder forms of HIV-associated neurocognitive disorders (HAND) is rising despite combination antiretroviral therapy (cART). Efavirenz (EFV) is among the most commonly used antiretroviral drugs globally, but causes neurological symptoms that may interfere with adherence and reduce tolerability, and may have central nervous system (CNS) effects that contribute in part to HAND in patients on cART. Thus we evaluated a commonly used EFV containing regimen: EFV/zidovudine (AZT)/lamivudine (3TC) in murine N2a cells transfected with the human “Swedish” mutant form of amyloid precursor protein (SweAPP N2a cells) to assess for promotion of amyloid-beta (Aβ) production (Chapter 3). Treatment with …

Effect Of Electronic Cigarette Messages On Young-Adult Behavioral Dispositions Towards Use, Idan Ariel

USF Tampa Graduate Theses and Dissertations

Over the last decade, electronic cigarettes (e-cigarettes) have emerged as novel and popular nicotine delivery devices. Although many smokers use e-cigarettes, evidence suggests these products are also growing in popularity among young adult non-smokers. It is therefore important to examine factors that may contribute to onset of electronic cigarette use among young adult non-smokers. Critics and supporters of electronic cigarettes have been disseminating anti and pro e-cigarette messages (respectively) and it is currently unclear what effect, if any, these messages exert on young adult non-smokers. Critics of electronic cigarettes advocate caution towards these products, while supporters of electronic cigarettes argue …

Tau-Directed Immunotherapy For Alzheimer’S Disease, Sulana Kay Schroeder

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is the leading cause of dementia, accounting for 50 to 80 percent of dementia cases, and the prevalence of the disease is projected to increase significantly with time. AD is characterized by severe cognitive decline with age, ultimately requiring continued caregiving and eventually death. The pathology of AD is characterized by the presence of extracellular amyloid plaques, intracellular neurofibrillary tangles (NFT) composed of hyperphosphorylated tau protein, neuron loss, and evidence of inflammation indicated by the presence of reactive microglia and astrocytes. Frontotemporal Lobe Dementia (FTLD) is a rare form of dementia that is related to AD, most …