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Bcl-2 Related Ovarian Killer, Bok, Is Cell Cycle Regulated And Sensitizes To Stress-Induced Apoptosis, José M. Rodríguez

USF Tampa Graduate Theses and Dissertations

Bok/Mtd (Bcl-2-related ovarian killer/Matador) is considered a pro-apoptotic member of the Bcl-2 family. Though identified in 1997, little is known about its biological role. We have previously demonstrated that Bok mRNA is upregulated following E2F1 over-expression. In the current work, we demonstrate that Bok RNA is low in quiescent cells and rises upon serum stimulation. To determine the mechanism underlying this regulation, we cloned and characterized the mouse Bok promoter. We find that the mouse promoter contains a conserved E2F binding site (-43 to -49) and that a Bok promoter-driven luciferase reporter is activated by serum stimulation dependent on this …

An Experimental Study Of Pro-Dieting And Anti-Dieting Psychoeducational Messages: Effects On Immediate And Short-Term Psychological Functioning And Weight Control Practices In College Women, Megan Roehrig

USF Tampa Graduate Theses and Dissertations

While dieting is relatively normative in our society, it is controversial within the fields of eating disorders and obesity. Dieting for weight loss has been touted by the obesity prevention field as a solution to the growing obesity epidemic, yet a body of research in the eating disorders field has also implicated it in the etiology and maintenance of eating pathology. Thus, a divergence in approaches toward dieting has emerged, with both prodieting and anti-dieting messages being recommended. Little is known, however, about the impact of these two types of messages on immediate and short-term psychological functioning and weight control …

Automatic Generation Of Global Phase Equilibrium Diagram From Equation Of State, Keyurkumar S. Patel

USF Tampa Graduate Theses and Dissertations

A computational tool that uses an automated and reliable procedure for systematic generation of global phase equilibrium diagram (GPED) is developed for binary system using equation of state and its extension to the ternary system is discussed. The proposed algorithm can handle solid phase and also can predict all major six types of phase diagrams. The procedure enables automatic generation of GPED which incorporates calculations of all important landmarks such as critical endpoints, quadruple point (if any), critical line, liquid-liquid-vapor line (if any), solid-liquid-liquid line (if any) and solid-liquid-vapor line. The method is also capable of locating all azeotropic phenomena …

Major Tea Catechin Inhibits Dendritic Cell Maturation In Response To Microbial Stimulation, James L. Rogers

USF Tampa Graduate Theses and Dissertations

Dendritic cells (DCs) are a migratory group of bone-marrow-derived leukocytes specialized for uptake, transport, processing and presentation of antigens to T cells. Exposure of DCs to bacterial pathogens can induce DC maturation characterized by cytokine production, up-regulation of co-stimulatory molecules and an increased ability to activate T cells. DCs have the ability to restrict growth of L. pneumophila (Lp), an intracellular Gram-negative bacillus that causes a severe form of pneumonia known as Legionnaires' disease, in murine ER-derived organelles (121) but replicate in human DCs (145). Even in human cells, however, lysis of the DCs does not occur for at least …

The Art Of Existentialism: F. Scott Fitzgerald, Ernest Hemingway, Norman Mailer And The American Existential Tradition, J'Aimã© L. Sanders

USF Tampa Graduate Theses and Dissertations

The purpose of my research is to examine the philosophic influences on three literary works: F. Scott Fitzgerald's The Great Gatsby, Ernest Hemingway's Death in the Afternoon, and Norman Mailer's An American Dream. Through an investigation of biographical, historical, cultural, and textual evidence, I will argue for the influence of several European philosophers---Friedrich Nietzsche, Søren Kierkegaard, and Martin Heidegger---on these authors and on the structures and messages of their works. I will discuss how the specific works I have selected not only reveal each author's apt understanding of the existential-philosophical crises facing the individual in the twentieth century, but also …

Perceptions Of Senior Faculty Concerning Doctoral Student Preparation For Faculty Roles, Jennifer M. Purcell

USF Tampa Graduate Theses and Dissertations

Calls for reform in doctoral education are not new. However, the past decade has experienced renewed interest and discussion in preparing the future professoriate. Whereas most studies of graduate student socialization and preparation for faculty roles have focused on doctoral students or new faculty, this study examined the perceptions of senior faculty members involved in doctoral education. All senior faculty (n=4970) in biological sciences, English, mathematics, and non-clinical psychology from a stratified sample of 69 research universities nationwide were invited to participate. More than 1150 faculty completed a web-based survey. Respondents rated the importance of 18 competencies (based on a …

The Assimilation Of The Marvelous Other: Reading Christoph Weiditz’S Trachtenbuch (1529) As An Ethnographic Document, Andrea Mckenzie Satterfield

USF Tampa Graduate Theses and Dissertations

This study examines the watercolor drawings of indigenous Americans in the Trachtenbuch, a small sixteenth-century manuscript by Christoph Weiditz. The manuscript was titled as a trachtenbuch by the Germanisches National Museum Library when cataloged in 1868, and Theodor Hampe published the first facsimile under this title in 1927. As this title suggests, the manuscript has long been narrowly defined and examined by scholars as a costume book. I argue instead for broadening the reading of the Trachtenbuch from a costume book, a subset of ethnographic documents that identify individuals based solely on systems of dress, to a visual ethnographic collection, …

Design Of Surface Acoustic Wave Sensors With Nanomaterial Sensing Layers: Application To Chemical And Biosensing, Subramanian K.R.S Sankaranarayanan

USF Tampa Graduate Theses and Dissertations

Surface acoustic wave (SAW) sensors detect chemical and biological species by monitoring the shifts in frequency of surface acoustic waves generated on piezoelectric substrates. Incorporation of nanomaterials having increased surface area as sensing layer have been effective in improving the sensitivity as well as miniaturization of SAW sensors. Selectivity, sensitivity and speed of response are the three primary aspects for any type of sensor. This dissertation focuses on design and development of SAW devices with novel transducer configurations employing nanomaterial sensing layers for enhanced sensing, improved selectivity, and speed of response. The sensing mechanism in these SAW sensors is a …

Reconstructing The Past: Heritage Research And Preservation Activities In Tampa Bay Communities, Courtney Ross Spillane

USF Tampa Graduate Theses and Dissertations

There are numerous ways in which cultural heritage can be preserved, such as: physical museums, virtual museums, tours of historic homes, and community meetings. For this project, I participated in and observed heritage preservation activities in two very different communities--- Sulphur Springs and Seminole Heights in Tampa, Florida. My internship appointment was with OSHNA (Old Seminole Heights Neighborhood Association) under the direction of Dr. Steve Gluckman. My primary focus was assisting heritage preservation committee members in each of the two communities with heritage preservation projects specific to their community needs and interests. One project is the development of a heritage …

The Proteoglycan Perlecan Regulates Long Bone Growth Through Interactions With Developmental Proteins In The Growth Plate, Simone Marsha-Lee Smith

USF Tampa Graduate Theses and Dissertations

Perlecan is the major heparan sulfate proteoglycan (HSPG) in growth plate cartilage and is critical for growth plate chondrocyte proliferation and proper skeletal development. Its core protein and attached chondroitin sulfate (CS) and heparan sulfate (HS) chains mediate interactions with many diverse proteins. Fibroblast growth factor (FGF)-2 and FGF-18 are other regulators of chondrocyte proliferation in the growth plate. Additionally, FGF-18 controls the hypertrophy and cartilage vascularization necessary for endochondral ossification. The research presented in this dissertation aimed to identify known and novel perlecan-binding proteins that are endogenous to the growth plate and to characterize their interactions with perlecan. FGF-2 …

The Effect Of Thin-Slicing On Structured Interview Decisions, Gregory F. Schmidt

USF Tampa Graduate Theses and Dissertations

This study examined whether hiring recommendations based on thin slices of a structured interview were associated with recommendations based on the interview in its entirety. After viewing 12 seconds of silent interviewee behavior, participants made hiring recommendations that were significantly correlated with those produced by individuals viewing a still-frame of the interview and the entire interview. In an effort to determine what sources of information participants were using to arrive at their recommendations, nonverbal behaviors were examined in detail. Applicants who appeared attentive, not anxious, competent, confident, dominant, optimistic, and professional were more likely to receive positive hiring recommendations than …

The Impact Of Management’S Tone On The Perception Of Management’S Credibility In Forecasting, Robert D. Slater

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to examine the impact of management altering its tone in communications on participants' perceptions of management credibility. Management's tone in communicating with participants was manipulated using communications from management under two treatment conditions. In period one of the study management's tone was manipulated within the management statement on internal controls as required by the Public Company Accounting Oversight Board's (PCAOB) Auditing Standards No. 2. In period one, participants had no knowledge of management's prior forecasting accuracy. Consistent with predicted hypotheses, the findings reveal that management can increase its credibility with participants by communicating its …

The Communicative Two-Way Pre-Writing Task Performed Via Asynchronous And Synchronous Computer-Mediated Communication And Its Influence On The Writing Expertise Development Of Adult English Language Learners: A Mixed Design Study, Iona Sarieva

USF Tampa Graduate Theses and Dissertations

This study addresses a gap in the second language writing research through examining processes occurring during the pre-writing and drafting stages of adult second language learners' writing when computer-mediated communication (CMC) pre-writing activities are involved. The theoretical framework adopted in the study is Writing-as-process approach with a focus being the pre-writing and drafting stages of the writing process. The design of the study is a parallel component mixed method design with an ongoing dominant qualitative stage and a nested less-dominant quantitative stage. In the qualitative stage of the project, two case studies were conducted: a group case study of the …

Scissors Paper Rock, Barbara St. Clair

USF Tampa Graduate Theses and Dissertations

Scissors Paper Rock is based on the true story of Amy Biel, a young and idealistic American woman who went to South Africa to support the political effort against Apartheid. She worked there as a volunteer organizer during a time of great political turmoil, and was days away from returning home to the United States, when she was violently killed in a riot. While Amy's death was not an accident--four men were found guilty for taking her life--it is certainly possible to think of her death as a tragedy. Amy's idealism and belief in her power to do good, allowed …

Crystal Engineering Of Co-Crystals And Their Relevance To Pharmaceutical Forms, Tanise R. Shattock

USF Tampa Graduate Theses and Dissertations

The research presented herein focus upon crystal engineering of co-crystals with an emphasis upon the exploration of co-crystals in the context of delineation of the reliability of hydrogen bonded supramolecular synthons and their hierarchies. The approach involves a combination of systematic Cambridge Structural Database analysis and a series of model co-crystal experiments. In addition, the viability of solid state methodologies toward supramolecular synthesis of co-crystals and the effect on polymorphism is also addressed. The application of the acquired knowledge is towards the crystal engineering of pharmaceutical co-crystals. The rational design and synthesis of pharmaceutical co-crystals accomplished by the selection of …

Pricing Models And Analysis Of Corporate Coupon-Bonds And Credit Default Swaptions, Michiru Shibata

USF Tampa Graduate Theses and Dissertations

In this work, pricing models of corporate coupon-bonds and credit default swaptions are derivedand analyzed. Corporate coupon-bonds are priced incorporating both intensity models and structural models, and also jumps introduced by seasonal effects. In deriving the models, we form portfolios to hedge the risk incurred by the instruments, then derive PDE equations using the arbitrage principle and the Ito Lemma for jump processes. The mathematical models are the parabolic-type PDE equations with terminal conditions and boundary conditions. These PDE problems are analyzed and solved by various transformations and incorporation with probabilistic properties. Either a unique solution in the exponential form …

Src Kinase Inhibitors For The Treatment Of Sarcomas: Cellular And Molecular Mechanisms Of Action, Audrey Cathryn Shor

USF Tampa Graduate Theses and Dissertations

Sarcomas are rare mesenchymally-derived tumors with limited treatment options. Tyrosine kinases may serve as potential targets for sarcoma therapy because many are mutated or overexpressed in sarcomas and cell lines. One potential molecular target for sarcoma treatment is the Src tyrosine kinase. Three independently synthesized Src kinase inhibitors were evaluated in human sarcoma cell lines. Of the three, dasatinib, provided promising results as a potential sarcoma therapy. Until this study, dasatinib activity had not been characterized in sarcoma cells. Based on our previous findings of Src activation in human sarcomas, we evaluated the effects of dasatinib in twelve sarcoma cell …

Construct Validity Of Situational Judgment Tests: An Examination Of The Effects Of Agreeableness, Organizational Leadership Culture, And Experience On Sjt Responses, Jonathan Adam Shoemaker

USF Tampa Graduate Theses and Dissertations

Numerous factors are likely to influence response patterns to situational judgment tests, including agreeableness, leadership style, impression management, and job and organizational experience. This research presents background information and research on situational judgment tests and several constructs hypothesized to influence situational judgment test responses. A situational judgment test and manipulations to influence response patterns were developed and piloted with a small sample of management professionals and undergraduate students. Larger samples of management professionals and undergraduate students participated in the experimental research. Participants were asked to imagine that they are applying for a job. Each participant was presented with background information …

How Do Proficient Intermediate Grade Writers Percieve Writing In School?, Tammy Weiss Schimmel

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine students' perspectives of writing instruction to gain insights into their awareness of the impact of high-stakes writing assessments on instructional practices and teaching strategies. Students in grades four and five who attended the 2004 Suncoast Young Author's Celebration (SYAC) served as the sample for this study. Data were gathered through surveys and interviews with 20 students who attended the SYAC. Survey questions were used to obtain general information about the students' perceptions of writing instruction and assessment. Interviews were conducted to gain a richer understanding of their perceptions of classroom experiences.The participants …

"A Woman's Face, Or Worse": Otto Rank And The Modernist Identity, Michael L. Shuman

USF Tampa Graduate Theses and Dissertations

Otto Rank is a significant but generally overlooked figure in the early history of psychoanalysis, and his work provides an illuminating context for the study of subjectivity and modernist culture. The "modernist identity" of my title is intended to represent, first, the concept of the individual self identified and expressed during this period and, secondly, the unique identity of modernist culture developed by artists through creative acts and emanating as the intellectual ambiance of the era. Through an examination of Rank's later theories and the work of prominent modernist artists, including Lawrence, Yeats, and Eliot, this dissertation will show that …

Psychometric Functions Of Clear And Conversational Speech For Young Normal Hearing Listeners In Noise, Jane Smart

USF Tampa Graduate Theses and Dissertations

Clear speech is a form of communication that talkers naturally use when speaking in difficult listening conditions or with a person who has a hearing loss. Clear speech, on average, provides listeners with hearing impairments an intelligibility benefit of 17 percentage points (Picheny, Durlach, & Braida, 1985) over conversational speech. In addition, it provides increased intelligibility in various listening conditions (Krause & Braida, 2003, among others), with different stimuli (Bradlow & Bent, 2002; Gagne, Rochette, & Charest, 2002; Helfer, 1997, among others) and across listener populations (Bradlow, Kraus, & Hayes, 2003, among others). Recently, researchers have attempted to compare their …

Greater Flexibility, Greater Growth: A Comparative Study Of Labor And Capitalist Models In Japan, Germany And The United States, Jay Arthur Thompson

USF Tampa Graduate Theses and Dissertations

After the end of the Second World War, three major economic powers emerged. Japan in Asia, Germany in Europe, and the United States in North America, quickly became the economic engines of their respective regions. Japan, with its "catch-up" and producer centered economy, grew so fast and so large, that there were worries in America that the Japanese would end up winning the economic war. West Germany, supported by the capitalist world, became a miracle economy, and the economic power of the European Union. In the past fifteen years however, these two economies have faltered and stagnated. In Japan, the …

Sensitivity Analysis Of The Transit Boardings Estimation And Simulation Tool (Tbest) Model, Dajana Vuckovic

USF Tampa Graduate Theses and Dissertations

Public transportation, although modest in the United States carrying about 2 percent trips, still serves millions of people as the main and only means of transportation. Recently released data set by Census, the 2006 American Community Survey (ACS) shows the main mode of travel for work commute is not surprisingly the automobile with over 86 percent and public transportation with nearly 5 percent users. Transit agencies strive to provide effective, convenient, and desirable transport. Because of the constant changes in our environment, being able to predict the response of riders to different network or system changes is extremely useful. Ridership …

The Proliferating Sacred: Secularization And Postmodernity, Donald Surrency

USF Tampa Graduate Theses and Dissertations

When analyzing the role religion plays in contemporary American society, one is forced to address the notion of secularization. This is a term that broadly refers to the marginalization of religious influence in culture and society that began at the outset of the Enlightenment, sometime during the sixteenth and seventeenth centuries, but, as Peter Berger argues, can actually be traced back to the times of the great Israelite prophets who emerged during the Axial Age (800-200 BCE). Throughout the available literature, proponents of the secularization thesis extend the marginalization of religious influence from the societal level to the level of …

Clinical Supervision Of Child And Adolescent Counselors In Residential Foster Care: A Collective Case Study, Lee A. Teufel

USF Tampa Graduate Theses and Dissertations

A collective case study is the study of more than one case (Stake, 1995). One therapist supervisor and three therapist supervisees from a child and adolescent residential foster care facility were observed during their individual clinical supervision and interviewed post-supervision. Currently, the literature on clinical supervision seldom addresses the supervision of working professional counselors; particularly those who are child and adolescent counselors (CACs).

Using a qualitative approach, two fundamental questions guided this inquiry: (a) what is the nature of clinical supervision involving a supervisor who provides clinical supervision to counselors in a child and adolescent residential foster care center that …

Androgen Receptors In The Bonnethead Shark, Sphyrna Tiburo: Cdna Cloning And Tissue-Specific Expression In The Male Reproductive Tract, John P. Tyminski

USF Tampa Graduate Theses and Dissertations

Androgens and the androgen receptor (AR) play important roles in virilization, spermatogenesis, and sexual behavior in vertebrates. An understanding of the distribution and levels of expression of the ARs on the cellular and tissue level demonstrates the pattern of responsiveness to the androgenic hormones in a given organism. In this study, a fragment of the AR gene was cloned and sequenced from the bonnethead shark, Sphyrna tiburo, an elasmobranch species with a well-defined annual reproductive cycle. Acquiring this gene sequence facilitated the construction of species-specific AR polymerase chain reaction (PCR) primers and species-specific AR mRNA probes that were used to …

Local Strategies In A Global Network: Disability Rights Advocacy In Jamaica, Joan A. Tucker

USF Tampa Graduate Theses and Dissertations

The declaration of the International Year of Disabled Persons in 1981 and the international activism of persons with disabilities resulted in the globalization of disability, which involved a new framework for the treatment of PWDs. Although disability activism was being played out globally, it has been about PWDs gaining equality and full participation in their local societies. Therefore, at the heart of an international movement was the strategic organization of PWDs, most of who live in the developing world. This dissertation is based on a case study of activism for disability rights in one particular developing society, Jamaica. The Jamaican …

The Relationship Between School Connectedness And Bullying Vicitmization In Secondary Students, Janet Urbanski

USF Tampa Graduate Theses and Dissertations

Bullying is a complex behavior that can cause academic and social problems for students and can contribute to a negative school climate. Students who feel isolated or do not feel connected to their school may experience similar risks to those who are victimized by peers. Recent school violence incidents have led to an increase in bullying behavior research. The importance of the school climate is also emerging in educational discourse prompting a growth of research in school connectedness and positive relationships. However, research on the impact that relationships and school connectedness may have on bullying victimization at school is limited. …

Assistive Force Feedback For Path Following In 3d Space For Upper Limb Rehabilitation Applications, Ramya Swaminathan

USF Tampa Graduate Theses and Dissertations

The primary objective of this research was the design of an easy to use C++ Graphical User Interface (GUI) which helps the user to choose the task that he/she wants to perform. This C++ application provides a platform intended for upper arm rehabilitation applications. The user can choose from different tasks such as: Assistive Function in 3D Space to Traverse a Linear Trajectory, User Controlled Velocity Based Scaling, Fitts' Task in X, Y, Z Directions. According to a study conducted by the scientific journal of the American Academy of Neurology, stroke patients aided by robotic rehabilitation devices gain significant improvement …

Uncertainty In The Information Supply Chain: Integrating Multiple Health Care Data Sources, Monica Chiarini Tremblay

USF Tampa Graduate Theses and Dissertations

Similar to a product supply chain, an information supply chain is a dynamic environment where networks of information-sharing agents gather data from many sources and utilize the same data for different tasks. Unfortunately, raw data arriving from a variety of sources are often plagued by errors (Ballou et al. 1998), which can lead to poor decision making. Supporting decision making in this challenging environment demands a proactive approach to data quality management, since the decision maker has no control over these data sources (Shankaranarayan et al. 2003). This is true in health care, and in particular in health planning, where …