Digital Commons Network^™

Molecular Mechanism Of Aurora-A Kinase In Human Oncogenesis, Lili He

USF Tampa Graduate Theses and Dissertations

Aurora-A is a mitotic kinase, which regulates cell cycle progression through modulating centrosome function. Aurora-A expression is frequently altered in human malignancies. The discrepancy between overexpression and amplification suggests that elevated Aurora-A level is likely to be regulated also by transcriptional and/or translational modifications. In this study, we have demonstrated: 1) transcriptional regulation of Aurora-A by E2F3; 2) feedback regulation between tumor suppressor CHFR and Aurora-A; 3) CNTD2 as a novel Aurora-A partner and oncogene to activate Aurora-A and induce transformation.

Aurora-A expression and activity are cell cycle regulated. The mechanism of Aurora-A upregulation at onset of mitosis is largely …

Investigating Environmental Change Due To Hypoxic Conditions On The Louisiana Continental Shelf: A Geochemical Approach, Marianne E. Dietz

USF Tampa Graduate Theses and Dissertations

The Louisiana (LA) shelf is chronically affected by seasonal hypoxia that has been shown to be spatially expanding and growing progressively more severe. Hypoxic conditions on the shelf have been closely linked to the large quantities of nutrients delivered to the Gulf of Mexico via the Mississippi River. Multiple geochemical proxies on a suite of sediment cores from the LA shelf provide a record of environmental change that parallels the onset of hypoxic conditions over the last century and prior to anthropogenic influences. The sedimentary record for the last century shows a shift from terrestrial to algal sources to the …

Low Temperature Polymer Electrolyte Fuel Cell Performance Degradation, John Andrew Fedock

USF Tampa Graduate Theses and Dissertations

The goal of this research was to quantify the degradation experienced by a polymer electrolyte fuel cell after storage at subzero temperatures ranging from 0 to -40°C. The performance loss was determined by comparing the polarization and other applicable power curves before and after the subzero storage cycle. The causes of this performance degradation were investigated by the use of Scanning Electron Microscope, Energy Dispersive x-ray Spectroscopy, and porosity scanning technologies. It was found that there are two distinct types of degradation experienced by the membrane. The first type was identified as a variance of the actual voltage - current …

Correlates And Predictors Of Cognitive Complexity Among Counseling And Social Work Students In Graduate Training Programs, Christopher Simmons

USF Tampa Graduate Theses and Dissertations

For this study, a web-based survey method was used as a means of collecting data to test a predictive model of education, supervised clinical experience (SCE), age, human services experience (HSE) and cognitive complexity. The theoretical framework for the study was Perry's (1970; 1999) scheme of intellectual development. The sample consisted of 332 counseling and social work students in graduate training programs in four different regions of the United States. The instruments used in the study were a researcher-developed demographic questionnaire and the Learning Environment Preferences (LEP) instrument (Moore, 1987). The results of the hierarchical regression analysis indicated that education …

Eutrophication Trend Of Lakes In The Tampa Bay Watershed And The Role Of Submerged Aquatic Vegetation In Buffering Lake Water Phosphorus Concentration, Max Jacobo Moreno Madriñan

USF Tampa Graduate Theses and Dissertations

Twentieth century human settlement within the Tampa Bay watershed was linked to a dramatic mid-century decline in bay water quality and loss of seagrass acreage. Decades of direct and indirect nutrient discharges to the bay from phosphorus mining, fertilizer manufacturing, and wastewater treatment, as examples, impaired the estuary. In the past twenty years, regional stakeholders have worked to improve the bay water quality by reducing point and non-point source nutrient loading to the bay.

Lakes within the Tampa Bay watershed may play an important role in attenuating the flow of nutrients into the bay. This study hypothesized that between 1990 …

Background Subtraction Using Ensembles Of Classifiers With An Extended Feature Set, Brendan F. Klare

USF Tampa Graduate Theses and Dissertations

The limitations of foreground segmentation in difficult environments using standard color space features often result in poor performance during autonomous tracking. This work presents a new approach for classification of foreground and background pixels in image sequences by employing an ensemble of classifiers, each operating on a different feature type such as the three RGB features, gradient magnitude and orientation features, and eight Haar features. These thirteen features are used in an ensemble classifier where each classifier operates on a single image feature. Each classifier implements a Mixture of Gaussians-based unsupervised background classification algorithm. The non-thresholded, classification decision score of …

Characterization Of The Western Antarctic Peninsula Ecosystem: Environmental Controls On The Zooplankton Community, Marina Marrari

USF Tampa Graduate Theses and Dissertations

The zooplankton community of Marguerite Bay, western Antarctic Peninsula, was investigated in relation to variability in chlorophyll concentrations and sea ice dynamics, using a combination of satellite remote sensing techniques and plankton net data. SeaWiFS chlorophyll data were validated with concurrent in situ data measured by HPLC and fluoromentric methods, and results indicate that SeaWiFS chlorophyll is an accurate measure of in situ values when HPLC data are used as ground truth.

Climatology data of SeaWiFS chlorophyll west of the Antarctic Peninsula showed that the Bellingshausen Sea and Marguerite Bay usually had higher and more persistent chlorophyll concentrations compared with …

Modulation Of Monocyte-Derived Dendritic Cell Maturation And Function By Cigarette Smoke Condensate In A Bronchial Epithelial Cell Co-Culture Model, Alison J. Montpetit

USF Tampa Graduate Theses and Dissertations

Lung airway epithelium is the first line of defense against inhaled particulates such as cigarette smoke. Subepithelial dendritic cells (DC) survey the airway epithelial lining and represent the link between innate and adaptive immune response. No study has investigated the effect of cigarette smoke on DC in the presence of epithelial cells (EC). The purpose of this 4x2 factorial design study was to co-culture normal human bronchial epithelial (NHBE) cells with monocyte-derived dendritic cells (MDDC) and examine the effect of cigarette smoke condensate (CSC) and poly I:C stimulation (TLR3 ligand that mimics viral infection) on MDDC homotypic clustering, phagocytosis ability, …

Beyond The Barriers: A Qualitative Investigation Into The Experiences Of General Pediatricians Working With Young Children Exhibiting Developmental Delays And Disabilities, Kahlila Genese Mack

USF Tampa Graduate Theses and Dissertations

General pediatricians are typically the first professionals to detect the early developmental concerns of young children during their infant and toddler stages. When concerns are identified by the general pediatrician, best practice encourages the referral of young children for further assessment and/or intervention. Due to these factors, this study focused on the methods general pediatricians use in their efforts to implement the American Academy of Pediatrics (AAP) guidelines for the developmental surveillance and screening of young children. Furthermore, this study focused on the barriers faced by general pediatricians and the solutions they have developed to overcome these barriers in their …

The Social Construction Of A Special Needs Program For Hurricanes, Robert E. Tabler Jr., M.A., C.H.E.S.

USF Tampa Graduate Theses and Dissertations

The overall purpose of this exploratory study was to comprehend how in the event of a hurricane Hillsborough County, Florida protected its elderly and disabled residents with special medical needs. This study used Social Constructionist Theory as a framework and Grounded Theory methodology in the collection of qualitative data.

To understand stakeholder knowledge and how they constructed the SpNP, three focus groups were conducted, with representatives from agencies on the Planning Committee. Through 30 in-depth, semi-structured interviews, clients of the SpNP, provided insight into their knowledge of the program and how society influenced evacuation decisions. Finally, 10 in-depth, semi-structured interviews …

Orbital- To Millennial-Scale Variability In Gulf Of Mexico Sea Surface Temperature And Salinity During The Late Pleistocene, Jessica L. Whitaker

USF Tampa Graduate Theses and Dissertations

Sea surface temperature (SST) reconstructions from the low latitudes indicate the tropics/subtropics warmed significantly before glacial-interglacial decreases in global ice volume, suggesting the importance of tropical and subtropical climate in driving glacial terminations.

ODP Site 625, drilled at a water depth of 889 m near De Soto Canyon in the Gulf of Mexico (GOM), provides continuous records of marine isotope stages (MIS) 1-6 sampled at a mean temporal resolution of 400 years. Age control is based on 8 AMS radiocarbon dates, marine isotope stratigraphy, and Foraminifera datum levels. Results from Globigerinoides ruber (white variety) Mg/Ca-SST indicate a rise of 4.4 …

The Far Reaching Impact Of Transformative Learning: A Critical Ethnographic Case Study, Aline E. Harrison

USF Tampa Graduate Theses and Dissertations

This multiple case study focused on the lives and experiences of four women who participated in an adult literacy program. This case study approach used critical ethnography as an analytic tool employing grounded theory leading to the development of a substantive theory. In-depth, semi-structured interviews and researcher's reflective journal were employed to collect data for this study that critically examined the impact of the transformative process of its participants and its influence on their socio-cultural context. Results revealed that participants did not necessarily experience a disorienting dilemma as contended by Mezirow (1978, 1991, and 2000). Rather participants experienced a series …

The Meaning And Means Of Inclusion For Students With Autism Spectrum Disorders: A Qualitative Study Of Educators’ And Parents’ Attitudes, Beliefs, And Decision-Making Strategies, Jenine M. Sansosti

USF Tampa Graduate Theses and Dissertations

The practice of inclusion, and even the term itself, have been the subject of controversy over the last several decades and it appears that "inclusion" may look very different depending upon the student, educator, and setting (Fuchs & Fuchs, 1994). Recently, placement in general education settings has become a dominant service delivery model for individuals with Autism Spectrum Disorder (ASD), (Simpson & Myles, 1998), yet Individual Education Programs (IEPs) for students with ASD tend to be the most often disputed and often contain procedural errors, including failure to consider the Least Restrictive Mandate (Yell et al., 2003).

This study represents …

Detection Of Marine Vehicles In Images And Video Of Open Sea, Sergiy Fefilatyev

USF Tampa Graduate Theses and Dissertations

This work presents a new technique for automatic detection of marine vehicles in images and video of open sea. Users of such system include border guards, military, port safety, flow management, and sanctuary protection personnel. The source of images and video is a digital camera or a camcorder which is placed on a buoy or stationary mounted in a harbor facility. The system is intended to work autonomously, taking images of the surrounding ocean surface and analyzing them for the presence of marine vehicles. The goal of the system is to detect an approximate window around the ship. The proposed …

Behavior Contracting With Dependent Runaway Youth, Jessica Colon

USF Tampa Graduate Theses and Dissertations

The number of dependent youth reported as runaways to the Florida Department of Law Enforcement has become an increasing concern to the Department of Children and Families (Child Welfare League of America, 2005). Youth under state supervision, who are reported as runaways, most often leave from foster care settings, although some youth are also reported as runaways from the homes of relatives, non-relatives, and biological parents (CWLA, 2005). Community based care (CBC) agencies responsible for the supervision of dependent children in the State of Florida have struggled to develop an effective means of addressing the problem of running away and …

A 1400 Year Multi-Proxy Record Of Hydrologic Variability In The Gulf Of Mexico: Exploring Ocean-Continent Linkages During The Late Holocene, Jennifer A. Flannery

USF Tampa Graduate Theses and Dissertations

Late Holocene climate variability includes the Little Ice Age (LIA, 450-150 BP) and the Medieval Warm Period (MWP, 1100-700 BP) that are characterized by contrasting hydrologic and thermal regimes. The degree of interaction between the North American continent and the ocean during these two abrupt climate events is not well known. Marine sedimentary records from basins proximal to major rivers integrate climate signals across large spatial scales and can provide a coherent, high-resolution assessment of the oceanic and continental responses to changing climate and hydrologic conditions. The Pigmy Basin in the northern Gulf of Mexico is ideally situated to record …

Publicly Posted Feedback With Goal Setting To Improve Tennis Performance, Gretchen Mathews

USF Tampa Graduate Theses and Dissertations

A review of the literature regarding Applied Behavior Analysis within various sports settings shows that behavioral coaching is more effective than traditional coaching methods. Specifically, goal setting and publicly posted feedback improved the athletic performance of college level football players in two studies, and high school soccer players in one study. The present study found goal setting and publicly posted feedback improved tennis performance for six participants. A multiple baseline across participants design was used. Specifically, the participants improved their corner hitting ability, which is an important competitive shot in tennis. The participants improved their corner hitting ability from an …

Determining The Quality And Impact Of An E-Mentoring Model On At-Risk Youth, Diane W. Culpepper

USF Tampa Graduate Theses and Dissertations

The purpose of this research was twofold. Since e-mentoring is relatively new, there have been very few studies that have explored the impact of an e-mentoring program on both the academic and psychological outcomes of its participants. In addition, there is little research on the quality of implementing, or what we will call the working quality, of an e-mentoring model. This study addressed both.

First, the study examined whether or not e-mentoring had an academic and psychological impact on 32 high school students who were at-risk of dropping out of school. The students were enrolled in a GED Exit Option …

Viruses Found In Raw Sewage And Their Potential To Indicate Fecal Pollution In Coastal Environments, Erin M. Symonds

USF Tampa Graduate Theses and Dissertations

The presence of pathogenic viruses in coastal environments is an important tool in evaluating water quality and health risks. Millions of viruses are excreted in fecal matter and bacterial indicators do not correlate with the presence of pathogenic viruses. Enteroviruses have been used to identify fecal pollution in the environment; however, other viruses shed in fecal matter could be used to indicate fecal pollution. The purpose of this research is to develop a baseline understanding of the diversity of viruses found in raw sewage and to assess their presence in the marine environment. PCR was used to detect adenoviruses, herpesviruses, …

Historical And Self-Imposed Asylums In Samuel Beckett’S Murphy, Malone Dies, And “First Love”, Suzanne Desmond

USF Tampa Graduate Theses and Dissertations

This thesis examines the uses and implications of historical and self-imposed asylums in several of Samuel Beckett's works, most notably Murphy, Malone Dies, and "First Love." The first half of this study compares several historical Irish and British asylums to Beckett's frictional institutions in order to illuminate the recurring motifs of sanity, asylums as retreats for the wealthy, and the links between prisons and asylums. I also examine Michel Foucault's theory of the Panopticon guards as an alternate reading of Beckett's views on sanity. In Murphy and Malone Dies, for example, Beckett questions what it means to be …

Life On The Campaign Trail: The Political Anthropology Of Local Politics, E.J Ford

USF Tampa Graduate Theses and Dissertations

This document is an ethnographic account of one researcher's experience during an election season spent with one candidate. The document considers the history of political anthropology as a subfield of anthropology, the deployment of ideology and hegemony as theoretical concepts, and includes a brief history of Tampa and Hillsborough County politics. The document attempts to make connections between the practical necessities of campaigning, with reference to the processual approach of examining micro-political process, and theoretical issues related to the subject of political anthropology, notably the concepts of ideology, hegemony, and the subject of elites in human social organization.

An Exploration Of The Sensemaking Process During The Implementation Of Academic Tracking Systems At Three Public Universities, Jean Mccarthy Gonzalez

USF Tampa Graduate Theses and Dissertations

Policy tends to be formulated at the upper level of an organization and handed down to policy actors on the frontline for enactment. Since policy can be vague, ambiguous, or send conflicting messages, it can create uncertainty about its meaning. Moreover, policy is rarely accompanied by explicit instructions for implementation. Therefore, when frontline workers, or streetlevel bureaucrats, are pressed to make sense of policy's meaning in realistic terms, they engage in what Weick describes as sensemaking (1995). Sensemaking is a cognitive process entered into during times of uncertainty or change. Streetlevel bureaucrats engage in sensemaking by drawing on cues in …

The Relationship Between Individual Differences In Cognitive, Social And Personality Development And The Increase In Complexity Of Children’S Alcohol Expectancies, Nicole M. Bekman

USF Tampa Graduate Theses and Dissertations

The current study aimed to simultaneously examine cognitive, social and personality development in a cross-sectional sample of 3^rd, 4^th and 5^th grade children to explore the interplay among these processes and how they relate to changes in children's understanding of alcohol. To replicate previous work, this study comprehensively examined relative increases in types of expectancies as a function of development. Results demonstrated that children in higher grades held more positive, negative and sedating expectancies of alcohol and positive alcohol expectancies increased more than negative alcohol expectancies. Improved performance on cognitive measures were associated with positive alcohol …

Measuring Undergraduate Student Perceptions Of Service Quality In Higher Education, Richard Scott Kelso

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine undergraduate student satisfaction with college services and environment at a large southeastern doctoral/research extensive university (target university), with the long-term intent of minimizing detractors to providing exceptional service quality, positively influencing customer satisfaction, and building loyalty intentions among students.

The ACT Student Opinion Survey (ACT, Inc.) was used to find the level of student satisfaction with the college services and environment. A stratified random sample of 468 undergraduate students responded to the survey. Three research questions guided the investigation. The study examined the general level of satisfaction with the support services, compared …

Teacher Efficacy And Student Achievement In Ninth And Tenth Grade Reading: A Multilevel Analysis, Anete Vasquez

USF Tampa Graduate Theses and Dissertations

More than 8 million of America's middle and high school students are struggling readers. Two-thirds of all eighth graders read below grade level, and the reading scores of high school students have not improved since 1974. Low literacy levels affect learning in all subject areas and impede student opportunity for future success. The No Child Left Behind Act of 2001 and the accountability measures associated with the Act have heightened public awareness of the deficiency in adolescent literacy. School districts are choosing to respond in one of two ways. Some school districts are opting to invest in teacher-proof curricula that …

Error Related Negativity In Parkinson’S Disease: A Test Of The Validity Of Mesencephalicdopamine Contributions To Ern, Craig A. Siders

USF Tampa Graduate Theses and Dissertations

A model proposed by Holroyd and Coles (2002) stating that error related negativity (ERN) is caused by a decrease in mesencephalic dopamine output to the ACC was tested. A group of individuals with Parkinson's disease (N = 16) and an age and education matched group free from neurological disorder (N = 16) completed a card guessing task where the magnitude of monetary penalties and rewards for incorrect and correct answers was varied by block. Individuals with Parkinson's disease were tested after an overnight washout from dopamingeric medications.

The amplitude of the mid-frontal negativity elicited by feedback was analyzed with spatial …

Utilitarian Approaches For Multi-Metric Optimization In Vlsi Circuit Design And Spatial Clustering, Upavan Gupta

USF Tampa Graduate Theses and Dissertations

In the field of VLSI circuit optimization, the scaling of semiconductor devices has led to the miniaturization of the feature sizes resulting in a significant increase in the integration density and size of the circuits. At the nanometer level, due to the effects of manufacturing process variations, the design optimization process has transitioned from the deterministic domain to the stochastic domain, and the inter-relationships among the specification parameters like delay, power, reliability, noise and area have become more intricate. New methods are required to examine these metrics in a unified manner, thus necessitating the need for multi-metric optimization. The optimization …

Adhesion Comparison Of Low Dielectric Constant Thin Films Using Four Point Bend And Nanoscratch Testing, Daniel Vilceus

USF Tampa Graduate Theses and Dissertations

As the semiconductor technology moves further into scaled down device structures, modern day complexities in the fabrication processes become more prevalent. This thesis focuses on the issues associated with mechaincal and adhesion failure in low dielectric constant (low-k) thin films. In this thesis the four point bend test and nanoscratch test method was used for evaluating adhesion of boro-phosphate-silicate glass (BPSG) and tetraethylorthosilicate (TEOS) low-k thin films to silicon subtrates. Nanoindation tests were also performed on the low-k films to evaluate material properties such as hardness and elastic modulus. The sample preparation and testing set up for the four point …

An Investigation Of The Online Learning Environment In Higher Education Through The Observations And Perceptions Of Students Of Color, Marie Adele Boyette

USF Tampa Graduate Theses and Dissertations

A need exists to gain a better understanding of how the online learning environment found in higher education is perceived by students of color. The research completed for this dissertation explored student participation in the online learning environment in higher education by examining the observations and perceptions of students of color. Along the way, the process of the research journey drew attention to the lack of inclusion of students of color in the literature and data collected about online learning, and pointed to the existence of the digital divide and its impact on graduate students of color and their participation …

Microwave Frequency Doubler Integrated With Miniaturized Planar Antennas, Suzette Marie Presas

USF Tampa Graduate Theses and Dissertations

In this thesis the development of a high efficiency harmonic re-radiator, consisting of a diode doubler and conjugate-matched receive and transmit antennas, is described. Diode-based frequency multipliers and rectifiers, coupled with antennas, are of interest for quasi-optical applications, for energy-scavenging and for sensing applications. The device studied operates by receiving an interrogating signal at a frequency of 1.3 GHz and re-radiating a signal at 2.6 GHz. The primary goal of this research was to develop a passive, miniature and effective frequency doubler integrated with planar antennas. The system is referred to as a frequency doubling reflectenna, (FDR). Prediction of accurate …