Digital Commons Network^™

Functional Roles Of Matrix Metalloproteinases In Bone Metastatic Prostate Cancer, Jeremy S. Frieling

USF Tampa Graduate Theses and Dissertations

Skeletal metastasis is a lethal component of many advanced cancers including prostate, the second most common cancer among men. Patients whose prostate cancer is localized and detected early benefit from multiple treatment options ranging from active surveillance to radiation and surgery, resulting in a 5-year survival rate of nearly 100%. Unfortunately, the prognosis and survival for patients with advanced metastatic disease is much worse due to the highly aggressive nature of the disease and a paucity of treatment options. Understanding the mechanisms and interactions that occur between metastatic cancer cells and the bone will enable the future treatment landscape for …

Geospatial And Negative Binomial Regression Analysis Of Culex Nigripalpus, Culex Erraticus, Coquillettidia Perturbans, And Aedes Vexans Counts And Precipitation And Land Use Land Cover Covariates In Polk County, Florida, Joshua P. Wright

USF Tampa Graduate Theses and Dissertations

Although mosquito monitoring systems in the form of dry-ice bated CDC light traps and sentinel chickens are used by mosquito control personnel in Polk County, Florida, the placement of these are random and do not necessarily reflect prevalent areas of vector mosquito populations. This can result in significant health, economic, and social impacts during disease outbreaks. Of these vector mosquitoes Culex nigripalpus, Culex erraticus, Coquillettidia perturbans, and Aedes vexans are present in Polk County and known to transmit multiple diseases, posing a public health concern. This study seeks to evaluate the effect of Land use Land cover …

Adaptive Region-Based Approaches For Cellular Segmentation Of Bright-Field Microscopy Images, Hady Ahmady Phoulady

USF Tampa Graduate Theses and Dissertations

Microscopy image processing is an emerging and quickly growing field in medical imaging research area. Recent advancements in technology including higher computation power, larger and cheaper storage modules, and more efficient and faster data acquisition devices such as whole-slide imaging scanners contributed to the recent microscopy image processing research advancement. Most of the methods in this research area either focus on automatically process images and make it easier for pathologists to direct their focus on the important regions in the image, or they aim to automate the whole job of experts including processing and classifying images or tissues that leads …

Estimating The Impact Of A Select Criteria Pollutant (Pm2.5) On Childhood Asthma In Florida, Shabnam Mehra

USF Tampa Graduate Theses and Dissertations

Asthma has been reported in children as a leading chronic illness in the US and around the world. It is also the third leading cause of hospitalization among children under the age of 15, and is also one of the most common causes of school absenteeism. Children are at higher risk of asthma attacks and they pose a higher burden on health care system. Nearly 20.6% of middle and high school children in Florida have been told they have asthma, this prevalence has grown over 3% from 2006 to 2012. Changes in air pollutant levels are often related to health …

Evolution, Ecology, And Disparities: Constructing Stature, Immune Functioning, And Reproduction In Brazilian Quilombo, And United States, Women, Anna C. Rivara

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation is to test how growth, reproduction, and immune functioning interact in two populations of adult women residing in vastly different socio-economic and ecological environments, the Kalunga quilombo in Brazil, and the United States of America. The presence of life history trade-offs was tested to determine how the different envirnonments, and socio-ecological contexts of the populations were creating differential risks for health and reproductive outcomes, and life history trade-offs.

I hypothesized that the Kalunga people, living in very difficult and harsh conditions, would experience greater amounts of, and more severe, life history trade-offs than the U.S. …

Semantic Description Of Activities In Videos, Fillipe Dias Moreira De Souza

USF Tampa Graduate Theses and Dissertations

Description of human activities in videos results not only in detection of actions and objects but also in identification of their active semantic relationships in the scene. Towards this broader goal, we present a combinatorial approach that assumes availability of algorithms for detecting and labeling objects and actions, albeit with some errors. Given these uncertain labels and detected objects, we link them into interpretative structures using domain knowledge encoded with concepts of Grenander’s general pattern theory. Here a semantic video description is built using basic units, termed generators, that represent labels of objects or actions. These generators have multiple out-bonds, …

Therapeutic Modulation Of Cancer Metabolism With Dichloroacetate And Metformin, Nathan Patrick Ward

USF Tampa Graduate Theses and Dissertations

The robust glycolytic metabolism of glioblastoma multiforme (GBM) has proven them susceptible to increases in oxidative metabolism induced by the pyruvate mimetic dichloroacetate (DCA). Recent reports demonstrate that the anti-diabetic drug metformin enhances the damaging oxidative stress associated with DCA treatment in cancer cells. We sought to elucidate the role of metformin’s reported activity as a mitochondrial complex I inhibitor in the enhancement of DCA cytotoxicity in the VM-M3 model of GBM. We demonstrated that metformin potentiated DCA-induced superoxide production and that this was required for enhanced cytotoxicity towards VM-M3 cells with the combination. Similarly, rotenone enhanced oxidative stress resultant …

Receptor-Associated Protein (Rap) Models In Vivo Reelin Haploinsufficiency: Implications In Schizophrenia, Jamileh Ahmed

USF Tampa Graduate Theses and Dissertations

The “two-hit” schizophrenia hypothesis suggests genetic and environmental abnormalities interrupt early CNS function. This increases vulnerability of a “second hit” and schizophrenia onset. Chronic stress and decreased Reelin signaling are reportedly associated with schizophrenia. Heterozygous Reeler Mice (HRM) show a 50% reduction in Reelin and display major schizophrenia phenotypes. Receptor-Associated Protein (RAP) blocks ligand-association to Reelin receptor Apolipoprotein E receptor 2 (ApoER2). In this study, we sought to replicate major heterozygous reeler mouse (HRM) phenotypes using in vivo RAP studies to establish an experimental in vitro model. Using an in vitro model, we investigated the effects of chronic stress and …

Non-Classical Regulators In Staphylococcus Aureus, Andy Weiss

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a highly problematic human pathogen due to its ability to cause devastating infections, paired with a capacity to withstand the action of a large fraction of available antibiotics. Both pathogenicity and antibiotic resistance are encoded by numerous genomic elements, though the expression of these factors is energetically costly and not always beneficial for cellular survival. Therefore, S. aureus has developed sophisticated regulatory networks to integrate a multitude of signals, enabling it to navigate the delicate balance between its pathogenic lifestyle and baseline needs for cellular energy homeostasis. It is thus imperative to study S. aureus behavior and …

Heteroglossic Chinese Online Literacy Practices On Micro-Blogging And Video-Sharing Sites, Yi Zhang

USF Tampa Graduate Theses and Dissertations

This study investigates Chinese online users’ adoptions of various languages and other meaning making signs in their online literacy practices in two popular Chinese CMC sites, Weibo (micro-blogging) and bilibili.com (video-sharing). Adopting the theoretical framework of heteroglossia (Bakhtin, 1981), I explore how various meaning making resources are creatively and playfully utilized by Chinese users in their online communication. After two-month data collection, I sampled the non-standard literacy practices (e.g., foreign language transliteration) identified from micro-blogging postings and comments in Weibo, as well as spontaneous (known as “bullet curtain” comments) and traditional text-box comments from featured videos in bilibili.com. The findings …

Learner-Directed Vs. Instructor-Provided Curriculum Among Undergraduate Students, Christopher D. Martinez

USF Tampa Graduate Theses and Dissertations

There has been little or no research on the use of a Learning Object in the field of religious studies. The purpose of this research study was to assess undergraduate student knowledge gain outcomes between instructor-provided and learner-directed methods, which is the independent variable, using asynchronous, online, narrated PowerPoint presentations compared to undergraduate student knowledge gain using an online learning-objects unit, in a college-level world religions survey course at St. Petersburg College. This study assessed undergraduate student knowledge gain outcomes between instructor-provided and learner-directed methods among 90 college undergraduate students and determined whether there was a significant difference in the …

Experimental Evaluation Of Cooling Effectiveness And Water Conservation In A Poultry House Using Flow Blurring® Atomizers, Rafael M. Rodriguez

USF Tampa Graduate Theses and Dissertations

Increases in population as well as economic improvements in developing countries are generating a larger demand for animal protein products. Current animal growth processes inherently, require the use of water in many forms throughout the growth cycle. Water is the most important natural resource on earth to sustain life, and in many developing countries is a scarce resource that must be used wisely. Studies have revealed that poultry growth can take place with less water consumption, when compared with other sources of animal protein (e.g., cattle, pork). In this research, an evaporative Flow Blurring^® cooling system was considered as …

The Influence Of Tropical Forests And Climate Change On The Fates Of Select Organic Pollutants In A Jamaican Watershed, Kayon Barrett

USF Tampa Graduate Theses and Dissertations

Many organic compounds, although beneficial, are associated with negative health and ecological impacts. It is therefore imperative to understand the environmental fates of these contaminants. Whereas the fates and health impacts of many persistent organic pollutants have been extensively examined, there is limited research characterizing the fates of these and the less persistent organic compounds in tropical multi-use watersheds. This study therefore aimed to evaluate the roles of forests and climate change on the environmental fates and health impacts of select organic chemicals in the Rio Cobre watershed, a tropical river basin in Jamaica. A total of 16 organic compounds …

Uncovering Transcriptional Activators And Targets Of Hsf-1 In Caenorhabditis Elegans, Jessica Brunquell

USF Tampa Graduate Theses and Dissertations

In order to survive, cells must be able to cope with a variety of environmental stressors. The heat shock response (HSR) is a pro-survival mechanism employed by cells in response to protein denaturing stress, such as heat. Since its discovery in 1960, the heat shock response has been found to be regulated by the transcription factor heat shock factor 1 (HSF1). During periods of increased stress, HSF1 undergoes a multi-step process of activation that involves homotrimerization, DNA-binding, and post-translational regulatory modifications, all of which ultimately function to control the transcription of chaperone genes. These chaperone genes encode molecular chaperone proteins …

“The Nations Of The Field And Wood”: The Uncertain Ontology Of Animals In Eighteenth-Century British Literature, J. Kevin Jordan

USF Tampa Graduate Theses and Dissertations

This dissertation analyzes the relationship between important intellectual discourses of the late-seventeenth and early-eighteenth centuries and the ontological status of non-human animals. The Enlightenment marks a distinct change in the ways in which humans gather knowledge and interact with the world, a change that forms the foundation for modern relationships between human and non-human animals. Through a theoretical framework that draws from animal studies and ecofeminism, I analyze the ways in which the status of non-human animals is shaped by the intersection of multiple anthropocentric concerns. In doing so, this dissertation probes the foundation of what defines the animal apart …

Acetaminophen Associated Neurotoxicity And Its Relevance To Neurodevelopmental Disorders, Seol-Hee Kim

USF Tampa Graduate Theses and Dissertations

Autism is a lifelong neurodevelopmental disorder. The etiology of autism still remains unclear due to the heterogeneous and complex nature of the disorder, however synergistic actions between genetic components and environmental factors have been suggested. Acetaminophen (APAP) is one of the most popular over-the-counter drugs that possess antipyretic and analgesic effects. It is considered a relatively safe and effective within therapeutic doses. Recently, early exposure to APAP has been suggested to be one of the underlying cause of autism. Children are often prescribed APAP to lessen fever or irritability after vaccination during the first year, and APAP may adversely affect …

Multi-Scale Approaches For Evaluating The Success Of Habitat Restoration In Tampa Bay, Florida, Stephanie Thompson Powers

USF Tampa Graduate Theses and Dissertations

This research aims to further the understanding of ecological restoration success in the Tampa Bay, Florida, region. Although over four hundred restoration projects have been completed in the bay area, knowledge of their success has been hindered by the lack of assessment and transfer of information concerning project outcomes. Without comprehensive project assessment, local science will be limited in its ability to inform practice because we lack the advantage of past knowledge.

Using a multi-scaled approach, a diverse set of restoration projects are evaluated, providing information on how the projects are contributing to defined targets established by the Tampa Bay …

Active Suffering: An Examination Of Spinoza's Approach To Tristita, Kathleen Ketring Schenk

USF Tampa Graduate Theses and Dissertations

Humans' capacity to attain knowledge is central to Spinoza's philosophy because, in part, knowing things enables humans to deal properly with their affects. But it is not just any sort of knowledge that humans should attain. There are different types of knowledge, but only two of them–rational and intuitive knowledge–enable humans who attain them to know things clearly. Because rational knowledge attends to universals whereas intuitive knowledge attends to particulars, intuitive knowledge is better than rational knowledge at enabling humans to deal with their affects. Most scholars recognize both the importance of knowledge to humans' dealing with their affects and …

Neuropeptide Modulation Of The Large Conductance Potassium (Bk) Channel In The Auditory System: Therapeutic Implications For Age-Related Hearing Loss, Ellliott James Brecht

USF Tampa Graduate Theses and Dissertations

The auditory temporal processing deficits associated with age-dependent hearing decline have been increasingly attributed to issues beyond peripheral hearing loss. Age-related hearing loss (ARHL), also known as presbycusis, is linked with changes in the expression of both excitatory and inhibitory neurotransmitters in the central auditory system. There are also age-related changes in the expression and function of the ion channels which mediate action potential firing. The slow, Ca²⁺ activated, K⁺ channels of the BK-type are essential in controlling both neurotransmitter release and neural communication via alteration of action potential durations, firing frequency, and neural adaptation. There are many …

The Sustainability Of Career And Technical Education Teacher Preparation Programs In Florida, Lisa Martino

USF Tampa Graduate Theses and Dissertations

There is a historical trend of decline in available Career and Technical Education (CTE) Teacher Preparation programs and an increase in CTE course and program enrollments in Florida’s secondary school system. This contradiction in CTE teacher supply and demand raises an important question. What is going on? More specifically, what sustains the remaining programs in the face of this decline?

The purpose of this qualitative study is to explore sustainability factors from past and present CTE teacher preparation program educators and administrators to gain a better understanding of this issue. The participant selection for this study was identified from four …

Acute Kidney Injury And Chronic Kidney Disease, Jin Wei

USF Tampa Graduate Theses and Dissertations

Ischemia and reperfusion are natural steps during kidney transplantation, and IRI is considered one of the most important nonspecific factors affecting allograft dysfunction. Transplanted organs experience several episodes of ischemia, in which cold ischemia occurs during allograft storage in preservation solutions.

Even though cold ischemia has been studied extensively, all of the studies have been carried out in vitro and ex vivo models. There is no in vivo model available to examine renal IRI induced solely by cold ischemia.

In the present study, we developed an in vivo mouse model to study renal IRI induced exclusively by cold ischemia through …

The Optimization Of The Nuclear Protein In Testis (Nut) Antibody And Its Importance And Impact In The Pathology Lab, Lindsey Martinez

USF Tampa Graduate Theses and Dissertations

Optimization in immunohistochemistry is often a time consuming and complex process. There are a varying array of moving parts to consider all while preserving the sensitivity and specificity of the test. When optimizing an antibody it is important to consider the fixation of the tissue and the type of epitope retrieval that would be best suited for the test. The dilution of the primary antibody is a key marker for the efficiency and effectiveness of the laboratory protocol. The purpose of this study was to produce an optimized antibody for the nuclear protein in testis to detect NUT midline carcinoma …

Speaking Of The Self: Theorizing The Dialogical Dimensions Of Ethical Agency, Bradley S. Warfield

USF Tampa Graduate Theses and Dissertations

This dissertation attempts to fill, in part, three lacunae in contemporary philosophical scholarship: first, the failure to identify the two distinct types of dialogism—psychological and interpersonal—that have been operative in discussions of the dialogical self; second, the lack of acknowledgement of the six most prominent features of interpersonal dialogism; and third, the unwillingness to recognize that interpersonal dialogism is a crucial feature of human ethical agency and identity.

In Chapter One, I explain why dialogism has been relatively neglected—and certainly underappreciated—in contemporary Western philosophy. In Chapter Two, I offer a picture of Mikhail Bakhtin’s conception of dialogism. I explain why …

Plasmonic Stimulation Of Electrically Excitable Cells, Fnu Parveen

USF Tampa Graduate Theses and Dissertations

There is a compelling need for the development of new sensory and neural prosthetic devices which are capable of more precise point stimulation. Current prosthetic devices suffer from the limitation of low spatial resolution due to the non-specific stimulation characteristics of electrical stimulation, i.e., the spread of electric fields generated. We present a visible light stimulation method for modulating the firing patterns of electrically-excitable cells using surface plasmon resonance phenomena. In in-vitro studies using gold (Au) nanoparticle-coated nanoelectrodes, we show that this method (substrate coated with nanoparticles) has potential for incorporating the technology into neural stimulation prosthetics, such as cochlear …