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Microbial Associations Of Four Species Of Algal Symbiont-Bearing Foraminifera From The Florida Reef Tract, Usa, Makenna May Martin

USF Tampa Graduate Theses and Dissertations

Marine microbiome research is a rapidly expanding field of study, as scientists investigate the functions of microbial associations in eukaryotic organisms. Foraminifera are among the most abundant shelled organisms in the oceans, yet little is known of their associated microbiomes. This study investigated microbes associated with four species of Foraminifera that host three kinds of algal endosymbionts. The Order Miliolida, Family Soritidae, was represented by three species: Archaias angulatus and Cyclorbiculina compressa, which both host chlorophyte symbionts, and Sorites orbiculus, which hosts dinoflagellate symbionts. The fourth species, Amphistegina gibbosa, belongs to the Order Rotaliida and hosts diatom endosymbionts. Bacterial DNA …

The Effects Of Gopher Tortoise (Gopherus Polyphemus) Herbivory On Plant Community Composition And Seed Germination, And The Effects Of Gut Passage On The Germinability Of Seeds: A Meta-Analysis, Jason C. Richardson

USF Tampa Graduate Theses and Dissertations

Herbivory produces direct and indirect effects on plants and at different spatial scales will have varied consequences. Consumption of plants by vertebrate grazers may affect the plants on an organismal level through direct mortality, on a community level by changing species composition or by altering the rate of succession, and even at a whole ecosystem level by altering nutrient cycles.

The majority of the scientific literature has focused extensively on herbivory by mammals and birds. With regard to mammals, studies have shown how folivory affects individual plants, plant populations, and communities of plants.

Mammals, as well as birds, also ingest …

Do All “Good Mothers” Breastfeed? How African American Mothers’ Values And Experiences Of Early Motherhood Influence Their Infant Feeding Choices, Airia S. Papadopoulos

USF Tampa Graduate Theses and Dissertations

The food an infant is fed can reflect many things: a source of nutrition, the social and cultural circumstances into which an infant is born, or even a family’s beliefs about the body and breast milk as a source of nutrition. Exclusive breastfeeding, currently the gold standard for infant feeding in the United States (US), is often identified as an expectation in discourses on being a “good mother.” African American mothers in particular are the least likely group in the US to breastfeed in any capacity and many efforts are underway to increase the breastfeeding rates of this population.

This …

Rumination And Worry: Factor Structure And Predictive Utility, Andrew Mark Kiselica

USF Tampa Graduate Theses and Dissertations

Criticism of discrete classification systems for mental disorders has led to a focus on identification of mechanisms that cut across symptom clusters, known as transdiagnostic factors. One such proposed factor is negative repetitive thought (NRT), or a perseverative, often uncontrollable, focus on negative information, experiences, or expectations. Worry and rumination are two major constructs thought to compose NRT. No confirmatory factor analyses have investigated whether worry and rumination might compose a general NRT factor, discrete factors, or some combination of the two. The first purpose of the current study was to use confirmatory factor analyses to uncover whether worry and …

Identification Of Novel Hits Against, Ala A. Azhari

USF Tampa Graduate Theses and Dissertations

Leishmaniasis is a disease caused by obligate intracellular parasites of the genus Leishmania, including 20 species that are pathogenic to humans. Female sand fly is the known vector that can transmit the disease. Visceral leishmaniasis is the severe form of the disease that affects internal organs and can be fatal with inappropriate diagnosis or treatment. Leishmania donovani is the causative agent of visceral leishmaniasis. Approximately 350 million in 89 countries are at risk of infection. Around 2 million new cases are reported annually with 500,000 of these are visceral leishmaniasis. Current drug therapies are inadequate due to their toxicity, high …

Targeting The Hsp90/Aha1 Complex For The Treatment Of Tauopathies, Lindsey Brooke Shelton

USF Tampa Graduate Theses and Dissertations

The microtubule associated protein, tau, is involved in regulating microtubule stability and axonal transport. When tau becomes hyperphosphorylated it can disassociate from the microtubules and start to aggregate. These tau aggregates are the hallmarks of many diseases known as tauopathies. The heat shock protein 90 kDa (Hsp90) chaperone network is highly involved in modulating client proteins, including tau. However, during aging and disease the Hsp90 chaperone network becomes highly imbalanced with some Hsp90/co-chaperone complexes increasing, while others are repressed. This imbalance in Hsp90/co-chaperone complexes could result in a worsening of tau pathology in Alzheimer’s disease.

Hsp90 inhibition has been of …

Tgf-Β2 In Human Milk Research: Exploration Of A New Field Methodology And New Findings Of Biosimilar Tgf-Β2 In Non-Human Milk, Chlöe A. Sweetman

USF Tampa Graduate Theses and Dissertations

Objectives: There are three aims for this thesis: the first is to develop a field and laboratory protocol for the storage and analysis of transforming growth factor–beta 2 (TGF-β2) in human breastmilk; second, to validate this protocol and the immunoassay used to assess this new method; and lastly, to explore the ramifications of biosimilar TGF-β2 across multiple milks on human health, growth, and immunity through the review of laboratory findings and previous literature.

Rational: Little anthropological research has been done on TGF-β2 in human milk. Anthropology as a discipline is well positioned to provide insight into TGF-β2, combining biocultural, evolutionary, …

Extending Spectrophotometric Pht Measurements In Coastal And Estuarine Environments, Nora Katherine Douglas

USF Tampa Graduate Theses and Dissertations

Nearshore and estuarine environments play a vital role in the cycling of carbon, but the effects of ocean acidification in estuarine waters have not been studied as extensively as in the open ocean. One reason for this is the limitation of pH measurement capabilities in low-salinity waters. Typically, pH in these environments has been measured using potentiometric methods that are subject to uncertainties on the order of 0.01. Spectrophotometric methods for measuring pHT offer precision and accuracy superior to those of potentiometric methods. However, previous characterizations for purified sulfonephthalein indicators, used for marine spectrophotometric measurements, are not applicable to estuarine …

Intersecting Stories: Cultural Reflexivity, Digital Storytelling, And Personal Narratives In Language Teacher Education, Julie Vivienne Dell-Jones

USF Tampa Graduate Theses and Dissertations

This narrative inquiry dissertation explores stories from three students over a two-year trajectory as they develop into language educators in diverse contexts. The study begins in a teacher education course focused on technology for language teaching in English as a second language (ESOL) and foreign language education (FLE) classrooms. As instructor, I implemented a digital storytelling (DS) project with the pedagogical goal of supporting the much-needed practice of reflexivity, and specifically, reflexivity of intercultural competence (IC) and culturally-responsive pedagogy (CRP). The DS, as an autoethnographic multimodal narrative activity, provided a creative outlet for undergraduate and master’s level students to explore …

From Florida To Antarctica: Dereplication Strategies And Chemical Investigations Of Marine Organisms, Matthew A. Knestrick

USF Tampa Graduate Theses and Dissertations

In the fight against disease and illness, nature has provided mankind some of our best therapeutics in the form of secondary metabolites. The plant, fungi and animal phyla inhabiting the Earth produce diverse and unique chemistry that can be used in our fight against disease. In the growing threat of drug resistance and pathogen evolution, the field of natural products chemistry strives to explore new biological and chemical diversity sources, and develop innovative methodology to identify and isolate new chemistry faster than ever.

The dissertation herein presented is one such effort to find new, bioactive chemistry from the marine environments. …

Identification Of Individuals From Ears In Real World Conditions, Earnest Eugene Hansley

USF Tampa Graduate Theses and Dissertations

A number of researchers have shown that ear recognition is a viable alternative to more common biometrics such as fingerprint, face and iris because the ear is relatively stable over time, the ear is non-invasive to capture, the ear is expressionless, and both the ear’s geometry and shape have significant variation among individuals. Researchers have used different approaches to enhance ear recognition. Some researchers have improved upon existing algorithms, some have developed algorithms from scratch to assist with recognizing individuals by ears, and some researchers have taken algorithms tried and tested for another purpose, i.e., face recognition, and applied them …

Characterization Of Scanning Mobility Particle Sizers For Use With Nanoaerosols, Michael R. Henderson

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to evaluate the performance of scanning mobility particle sizers in the characterization of nanoaerosols. A sampling chamber was constructed from aluminum and tempered glass, had a volume of 4.6 cubic feet, and was designed for the introduction of aerosols and dilution air, maintenance of aerosol concentration, and continuous exhaust of chamber air. Penetration and aerosol distribution tests were conducted within the chamber. An aerosol generation and measurement system comprised of nitrogen gas, BGI 3 jet Collison Nebulizer, diffusion dryer, aerosol charge neutralizer, mixing chamber, critical orifice, hygrometer, condensation particle counter, scanning mobility particle sizer, …

Investigation Of Alcohol-Induced Changes In Hepatic Histone Modifications Using Mass Spectrometry Based Proteomics, Crystina Leah Kriss

USF Tampa Graduate Theses and Dissertations

Alcohol liver disease (ALD) is a major health concern throughout the world. Currently, in the United States, 17 million people suffer from alcoholism, of which 1.4 million people are receiving treatment [1, 2]. The link between ethanol metabolism, reactive oxygen species (ROS) and liver injury in ALD has been well characterized over the last couple decades [3-10]. Ethanol metabolism relies on the availability of the cofactor NAD+ for the oxidation of ethanol into acetate, consequently causing alterations in redox potential. Redox dysfunction within the mitochondria can affect multiple pathways important in maintaining cellular homeostasis. Chapter 1 provides an introduction to …

The Role Of Phosphohistidine Phosphatase 1 In Ethanol-Induced Liver Injury, Daniel Richard Martin

USF Tampa Graduate Theses and Dissertations

Chronic liver diseases, which includes alcoholic liver disease (ALD), are consistently among the top 15 leading causes of death in the United States. ALD is characterized by progression from a normal liver to fatty liver disease (hepatic steatosis), which can lead to cirrhosis, alcoholic hepatitis, and liver failure. We have identified a novel role of phosphohistidine signaling, mediated through phosphohistidine phosphatase 1 (PHPT1), in the onset of hepatic steatosis. We have identified PHPT1 as a target of selective oxidation following acute ethanol exposure as well as being downregulated following chronic ethanol exposure. We mapped the oxidative modification site and developed …

Animated Pedagogical Agent’S Roles And English Learners’ Prior Knowledge: The Influence On Cognitive Load, Motivation, And Vocabulary Acquisition, Fadwa Yasin Flemban

USF Tampa Graduate Theses and Dissertations

Researchers and educators have always strived for creating appropriate instructional tools and resources that help students to acquire knowledge. Animated pedagogical agents (APAs) embedded within multimedia learning settings are one of the emerging technologies that provide a powerful and supportive learning environment. According to previous studies, APAs can effectively promote learning and support social interaction with learners (Johnson & Lester, 2016; Lane, 2016). However, APAs also may cause cognitive load without providing motivational benefits in some cases and distract learners during the learning process. In other words, the results of previous studies on APAs do not provide enough evidence to …

Becoming A Woman Of Isis, Zoe D. Fine

USF Tampa Graduate Theses and Dissertations

In this study, I examine how terrorism is produced and consumed in communication. Using discourse analysis, I investigate how terrorism is constituted in the accounts of four women described in online news reports as having joined, or almost joined the so-called Islamic State (IS): “Alex,” constructed as having been lonely and flirted with IS; “Khadija,” presented as a schoolteacher turned member of IS’s all-women’s brigade; Laura, described as a woman whose partner abandoned her, who met a man online, and who brought her son with her to join IS; and Tareena, referred to as a health worker who brought her …

A Novel Framework To Determine Physiological Signals From Blood Flow Dynamics, Prashanth Chetlur Adithya

USF Tampa Graduate Theses and Dissertations

Centers for Disease Control and Prevention (CDC) estimate that more than 11.2 million people require critical and emergency care in the United States per year. Optimizing and improving patient morbidity and mortality outcomes are the primary objectives of monitoring in critical and emergency care. Patients in need of critical or emergency care in general are at a risk of single or multiple organ failures occurring due to a traumatic injury, a surgical event, or an underlying pathology that results in severe patient hemodynamic instability. Hence, continuous monitoring of fundamental cardiovascular hemodynamic parameters, such as heart rate, respiratory rate, blood pressure, …

Synthesis Of Novel Agents For The Treatment Of Infectious And Neurodegenerative Diseases, Benjamin Joe Eduful

USF Tampa Graduate Theses and Dissertations

Infectious and neurodegenerative diseases continue to be a major concern worldwide. In spite of the great advances in drug therapy for treating various infectious and neurodegenerative diseases, there is still an urgent need for new and improved drugs due to increasing drug resistance among pathogens, emergence of new pathogens, ease of transmission of infections, ineffective available treatments, toxicity associated with current standard of care, aging populations and the lack of better alternative treatment options.

The first part of this manuscript (chapters 1 - 5) describes the synthesis of novel agents active against Leishmania donovani. According to the World Health Organization …

Neural Mechanisms Of Transcranial Magnetic Stimulation In The Treatment Of Tinnitus, Andrea S. Lowe

USF Tampa Graduate Theses and Dissertations

Millions of people suffer from tinnitus, a disorder for which there is currently no effective treatment or cure. My dissertation work provides insight into the neural correlates of this pervasive hearing disorder and examines how a newly emerging therapy, transcranial magnetic stimulation (TMS), affects the central auditory system in the generation of the tinnitus percept. This work has a multifold focus of: i) developing and modeling the function of a miniature magnetic coil that can be used for TMS in rodents, ii) establishing a reliable mouse model of tinnitus that can be used for assessing TMS treatment-induced changes, iii) measuring …

Climate Change: Aristotelian Virtue Theory, The Aidōs Response And Proper Primility, John W. Voelpel

USF Tampa Graduate Theses and Dissertations

Climate change is the first anthropogenic alteration of a global Earth system. It is globally catastrophic in terms of food production, sea level rise, fresh water availability, temperature elevation, ocean acidification, species disturbance and destruction to name just a few crisis concerns. In addition, while those changes are occurring now, they are amplifying over decadal periods and will last for centuries and possibly millennia. While there are a number of pollutants involved, carbon dioxide (CO2) which results from the combustion of any fossil fuel is the primary pollutant. It has not been considered a pollutant until recently because of its …

A Fall Prevention System For The Elderly And Visually Impaired, Yueng Santiago De La Hoz Isaza

USF Tampa Graduate Theses and Dissertations

The World Health Organization claims that there are more than 285 million blind and visually impaired people in the world. In the US, 25 million Americans suffer from total or partial vision loss. As a result of their impairment, they struggle with mobility problems, especially the risk of falling. According to the National Council On Aging, falls are among the primary causes for fatal injury and they are the most common cause of non-fatal trauma-related hospital admissions among older adults. Visibility, an organization that helps visually impaired people, reports that people with visual impairments are twice as likely to fall …

Selection Of Energy Systems In Aquaculture Through A Decision Support Tool Considering Economic And Environmental Sustainability, Youngwoon Kim

USF Tampa Graduate Theses and Dissertations

Aquaculture had already been distinguished as an important component of global food security and economics. However, aquaculture has expanded at the cost of natural resources and the environment. The vulnerability of the aquaculture industry due to the consequences of global environmental changes and energy price fluctuations has been addressed in various studies. The identification, planning, and implementation of sustainable energy systems are important to ensure the long term economic and environmental sustainability of aquaculture.

This research investigated sustainable energy systems for aquaculture using a life cycle approach, allowing for the identification of the most sustainable energy options under different geographical …

Curating Illustrations Of Lewis Carroll’S Alice’S Adventures In Wonderland, Lindsay Persohn

USF Tampa Graduate Theses and Dissertations

In the 150 years since Lewis Carroll and John Tenniel (1865/1866) first published Alice's Adventures in Wonderland, various illustrators have found inspiration in this story to recreate its images again and again. Since Carroll and Tenniel, Wonderland has concerned itself with sociocultural ideas and the work of artists who re-illustrated this story provide ways to trace history of these ideas.

Accordingly, the purpose of this project was to examine connections and breaks with tradition in illustration that contribute to an evolution of meaning in the Wonderland story. Additionally, through this project, I worked to interpret ideas from different artists in …

School Counselors' Role In Reducing Aggression In Children In Alternative Education Programs, Deirdra Sanders-Burnett

USF Tampa Graduate Theses and Dissertations

Despite efforts to reduce the occurrence of physical aggression in traditional school environments, some students persist in engaging in physical aggression and are assigned to alternative education programs as an alternative to expulsion. However, relatively little is known about the types of services that school counselors provide to help these students. This case study investigated the services that school counselors provide to the young children attending disciplinary alternative education programs in a public-school district in central Florida. The participants consist of two school counselors who provided services to the students assigned to the alternative disciplinary programs and two district-level administrators …

Remote Sensing And Spatial Metrics For Quantifying Seagrass Landscape Changes: A Study On The 2011 Indian River Lagoon Florida Seagrass Die-Off Event, René Dieter Baumstark

USF Tampa Graduate Theses and Dissertations

Florida’s seagrasses are ecologically important marine environments which have suffered major degradation caused by increasing anthropogenic pressures. A 2011 seagrass die-off event caused by an algal bloom in the Florida Indian River Lagoon (IRL) was particularly severe with a majority of seagrass lost in areas such as the Banana River. An understanding of how this coastal marine environment changed is an important step toward better managing resources for conservation. Modern tools and methods provide new opportunities to study these changes at the landscape scale, a scale that informs on the larger more comprehensive state of a system. Classified satellite imagery …

Operationalizing The Pressure And Release Theoretical Framework Using Risk Ratio Analysis To Measure Vulnerability And Predict Risk From Natural Hazards In The Tampa, Fl Metropolitan Area, Jessica A. Wilder

USF Tampa Graduate Theses and Dissertations

Significant damage and loss is experienced every year due to natural hazards such as hurricanes, tornadoes, droughts, floods, wildfires, volcanoes, and earthquakes. NOAA’s National Center for Environmental Information (NCEI) reports that in 2016 the United States experienced more than a dozen climate disaster events with damages and loss in excess of a billion dollars (NOAA National Centers for Environmental Information, 2017). Identifying vulnerabilities and risk associated with disaster threats is now a major focus of natural hazards research. Natural hazards research has yielded numerous theoretical frameworks over the last 25 years that have explained important elements of risk and vulnerability …

Assessment Of Drinking Water Quality Management And A Treatment Feasibility Study For Brick By Brick Water Storage Tanks In Rakai Uganda, James V. Murduca

USF Tampa Graduate Theses and Dissertations

Reliable access to safe drinking water is one necessity for humans to live without concern for major health risks. The overall goal of this research is to improve the public health, through improved drinking water, for communities in the Rakai District in Uganda, directly, and other communities in the world, indirectly, via dissemination of knowledge. This study specifically assessed the knowledge of drinking water quality in regards to public health, their sanitation measures, and water treatment methods for users of Brick by Brick rainwater harvesting tanks in the Rakai District (N = 28) by using a knowledge, attitudes, and practice …

Identification Of Regulatory Mirnas Associated With Ethanol-Induced Microglial Activation Using Integrated Proteomic And Transcriptomic Approaches, Brandi Jo Cook

USF Tampa Graduate Theses and Dissertations

Chronic consumption of, and acute intoxication from, alcohol can have profound effects on the functional integrity of the central nervous system (CNS). The resident immunomodulatory cells of the CNS, microglia, provide signaling factors with both pro- and anti-inflammatory effects for protection. Microglial activation ranges through a multiplex of phases, of which have yet to be defined when induced by exposure to alcohol, and how the activation impacts surrounding cells. Exposure of alcohol has been revealed to induce an immune response in microglia, which can exhibit characteristics unique to a pro-inflammatory response depending on dose and time of alcohol exposure. To …

Molecular Phylogenetics Of Floridian Boletes, Arian Farid

USF Tampa Graduate Theses and Dissertations

The boletes are macrofungi which have undergone extensive taxonomic revisions since the advent of molecular tools. To further our understanding of the boletes in peninsular Florida, we sequenced two common Floridian boletes, and analyzed them with molecular phylogenetic tools. Boletus rubricitrinus, a common Florida bolete often found in lawns under Quercus, and likely has a distribution that extends to Texas. Based on ITS and LSU sequences and morphological studies, this species belongs in the genus Pulchroboletus. As the holotype is in poor condition, an epitype is established here. A thorough description of macroscopic and microscopic features is also provided for …

Impact Of Substrate To Inoculum Ratio On Methane Production In High Solids Anaerobic Digestion (Hs-Ad) Of Food Waste, Yard Waste, And Biosolids, Phillip James Dixon

USF Tampa Graduate Theses and Dissertations

High solids anaerobic digestion (HS-AD) is an alternative for managing the organic fraction of municipal solids waste (MSW), which produces mainly methane (CH4) and fertilizer as byproducts. HS-AD offers a potentially more economically and environmentally sustainable option compared with landfilling or incineration waste-to-energy facilities. However, HS-AD is a complex process requiring specific microbial communities working together symbiotically. Previous studies have found that the substrate to inoculum (S/I) ratio affects CH4 production and yield in HS-AD reactors by affecting substrate mass and energy transfer as well as microbial activity. In this thesis, biochemical methane potential (BMP) assays were used to investigate …