Digital Commons Network^™

Development And Application Of Decision-Making Tool For Rural Spring-Sourced Gravity-Fed Community Managed Water Systems, Oswaldo Galicia

USF Tampa Graduate Theses and Dissertations

People all over the world still lack access to safe drinking water service. Those with access experience several issues during the first few years of installation that impede on their overall access. In order to improve water services, not only is a proper decision-making tool necessary, taking into account key factors that impact sustainable water service, but proper monitoring and evaluation is also important in ensuring service for the long term. There are several developed assessment tools used for monitoring and evaluation of water systems post-construction, applicable in various scenarios. However, there are only few tools available to facilitate the …

Understanding Immunometabolic And Suppressive Factors That Impact Cancer Development, Rebecca Swearingen Hesterberg

USF Tampa Graduate Theses and Dissertations

The immune system is responsible for surveillance against pathogens and malignancies and adoptive transfer of antigen-specific T is a powerful new cancer treatment option. There is a clinical need to understand more about the normal regulatory processes that prevent immunosurveillance and how to manipulate this for clinical benefit. This project aims to understand T cells from three different angles. First, the biological role of the negative regulator cereblon (CRBN) was investigated in CD8+ T cells and my studies showed that higher levels of specific derivatives downstream of the amino acid glutamine may be beneficial to T cells in the immune …

Using Class Pass Intervention (Cpi) To Decrease Disruptive Behavior In Children, Andrea N. Zuniga

USF Tampa Graduate Theses and Dissertations

Finding of previous research has shown that disruptive behavior can impair students’ academic success (Pierce, Reid, & Epstein, 2004), as well as increase teacher’s stress level (Westling, 2010). Class Pass Intervention (CPI) is a Tier 2 intervention designed to decrease disruptive behavior and increase academic engagement, however, thus far research on the effects of CPI has been limited to typically developing elementary and high school students with escape and attention-maintained problem behaviors. Therefore, the purpose of this study was to replicate and extend previous research on the effects of CPI on problem behavior and academic engagement however with students whose …

Translational Evaluation Of History Effects On Resurgence, Paige L. Talhelm

USF Tampa Graduate Theses and Dissertations

Resurgence is the reappearance of an extinguished operant response when an alternative behavior is subsequently treated with extinction (Podlesnik & Shahan, 2009). A potential solution to this problem is training serial alternative responses. During the present study, undergraduate students were trained to engage in an arbitrary response analogous to problem behavior and two alternative responses. Each response was reinforced for a distinct duration to establish different reinforcement histories and then tested under conditions of resurgence. The purpose of the present study was to evaluate the effects of duration of reinforcement on behavior subsequently exposed to resurgence contingencies. Three subjects engaged …

Evaluation Of Novel Hemocompatible Surface Coatings For Extracorporeal Life Support: A Biocompatible Alternative To Systemic Anticoagulation, Teryn Rose Roberts

USF Tampa Graduate Theses and Dissertations

Extracorporeal life support (ECLS) is a class of technologies used to support or replace the function of failing organs. During ECLS, blood is withdrawn from systemic circulation and circulated through an artificial organ or “treatment membrane” that performs the function of the failing organ, prior to return to systemic circulation. While ECLS provides life-saving therapy to wide patient populations from pre-term infants to combat-wounded soldiers, this therapy is limited due to secondary thrombotic and bleeding complications that result from: 1) exposure of blood to the foreign surfaces in the device circuit and 2) administration of anticoagulant drugs to prevent clot …

The Case For The Green Kant: A Defense And Application Of A Kantian Approach To Environmental Ethics, Zachary T. Vereb

USF Tampa Graduate Theses and Dissertations

Environmental philosophers have argued that Kant’s philosophy offers little for environmental issues. Furthermore, Kant scholars typically focus on humanity, ignoring the question of duties to the environment. In my dissertation, I turn to a number of underexploited texts in Kant’s work to show how both sides are misguided in neglecting the ecological potential of Kant, making the case for the green Kant at the intersection of Kant scholarship and environmental ethics. I build upon previous literature to argue that the green Kant matters for both sides. Rather than a liability, Kant is indeed a conceptual resource. Though many conceive of …

Mechanisms Of S1p-Induced Endothelial Barrier Enhancement, Natascha Guimarães Alves

USF Tampa Graduate Theses and Dissertations

Excessive microvascular permeability is a serious complication involved in traumatic injury and inflammatory diseases. Alcohol intoxication can exacerbate the physiological derangements produced by microvascular endothelial barrier dysfunction in such disease conditions. Sphingosine-1-phosphate (S1P) has known endothelial barrier-protective properties, and has been shown to ameliorate microvascular leakage in a model of combined alcohol intoxication and hemorrhagic shock and resuscitation (HSR). However, whether the barrier-protective properties of S1P extend to endothelial cells of the blood-brain barrier (BBB) is unclear. The mechanisms of S1P-induced barrier protection during alcohol intoxication or HSR are also unknown. In the current study, we tested the hypothesis that …

High-Precision Lead Isotope Analysis On Modern Populations To Determine Geolocation Reliability, Gennifer M. Goad

USF Tampa Graduate Theses and Dissertations

Forensic anthropologists increasingly use chemical isotope analysis in the investigation of unidentified human remains, as biochemical georeferencing continually improves with the development of modern reference data of known origins. Isotope variations in trace elements such as strontium (Sr) and lead (Pb) in human teeth are some of the most useful indicators of past domicile in archaeological research and thus have high potential for modern, forensic applications. In this study, high-precision lead isotope analysis was conducted on 63 modern human teeth, which were previously analyzed for strontium isotopes. The results present new lead isotope data for the following countries: United States …

Human Body Motions Optimization For Able-Bodied Individuals And Prosthesis Users During Activities Of Daily Living Using A Personalized Robot-Human Model, Dimitrios Menychtas

USF Tampa Graduate Theses and Dissertations

Current clinical practice regarding upper body prosthesis prescription and training is lacking a standarized, quantitative method to evaluate the impact of the prosthetic device. The amputee care team typically uses prior experiences to provide prescription and training customized for each individual. As a result, it is quite challenging to determine the right type and fit of a prosthesis and provide appropriate training to properly utilize it early in the process. It is also very difficult to anticipate expected and undesired compensatory motions due to reduced degrees of freedom of a prosthesis user. In an effort to address this, a tool …

Fatty Acid Amides And Their Biosynthetic Enzymes Found In Insect Model Systems, Ryan L. Anderson

USF Tampa Graduate Theses and Dissertations

A fatty acid amide is precisely as the name suggests: A fatty acid (CHn-COOH), in which the hydroxyl group of the carboxylic acid is displaced by an amine functional group from a biogenic amine (R-NH2), ultimately forming an amide bond. Furthermore, these fatty acid amides can be composed of a variety of different acyl chain lengths donated by the fatty acid and a myriad of different biogenic amines. Thus, these molecules can be subdivided in a number of different ways including the separation of short chain (acetyl to heptanoyl) and long chain (palmitoyl to arachidonoyl) and also based off the …

Actinomycin D And Telmisartan Combination Therapy Targets Lung Cancer Stem Cells, Ryan Green

USF Tampa Graduate Theses and Dissertations

The failure of lung cancer treatments has been attributed partly to the development of drug resistance, however the underlying cellular and molecular mechanisms are poorly understood. It has been suggested that a very small group of specific cells within the heterogeneous tumors, cancer initiating stem cells (CSC), develop resistance to treatment, survive and later initiate the growth of new tumors. Due to their pivotal role in maintenance and relapse of tumors following the acquisition of drug resistance, we reasoned that novel drugs targeting cancer cells and CSC might provide the most effective treatments, if not a cure. To this end, …

Border-Crossing Travels Across Literary Worlds: My Shamanic Conscientization, Scott Neumeister

USF Tampa Graduate Theses and Dissertations

Border-Crossing Travels Across Literary Worlds is an autocritographical journey that places a group of U.S. literary texts into critically conscious dialogue with the “text” of my life. As a white, American, middle-class, cishetero, able-bodied man, I historicize, contextualize, analyze, and deconstruct the process by which my ten years of graduate academic studies at the University of South Florida fostered my ongoing awakening to critical consciousness—the personal and political evolution Paolo Freire terms “conscientization.” I present the analytical insights I realized about landmark feminist and womanist texts I encountered during my graduate studies that resonate with the prominent literary works and …

Beauty And The Beasts: Making Places With Literary Animals Of Florida, Haili A. Alcorn

USF Tampa Graduate Theses and Dissertations

Place theory examines the relationship between human identity and physical locations, asking how meaningful attachments are formed between people and the spots they visit or in which they live. Literature of place exhibits this relationship and the myriad ways humans connect to their environment through storytelling, both fictional and nonfictional. Florida literature, an emerging and dynamic genre, features characters, cultures, and histories heavily embedded in place. Florida’s places also abound with animal presences, and literature about Florida almost always illustrates significant human-animal interactions that drive plots and character development. Therefore, Florida literature invites consideration of how animals influence human attachment …

Proteolysis Of Cx3cl1 Impacts Cx3cr1 Signaling And Therapeutic Benefits In A Tauopathy Model, Dylan John Finneran

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is a progressive, neurodegenerative disorder and the most common form of dementia. The hallmark pathologies of AD are extracellular aggregates of amyloid-beta, intracellular aggregates of microtubule associated protein tau and increased neuroinflammation. Current therapeutics offer only symptomatic relief and clinical trials investigating therapeutic benefits of non-steroidal anti-inflammatory drugs have yielded no positive results. Therefore, recent work has focused on immunomodulators, such as CD200 and fractalkine, as potential therapeutic targets for AD.

Fractalkine (CX3CL1; FKN) is expressed as a transmembrane protein with an N-terminal chemokine domain followed by a long, mucin-like stalk. FKN can signal as a membrane-bound …

Investigating The Roles Of Fucosylation And Calcium Signaling In Melanoma Invasion, Tyler S. Keeley

USF Tampa Graduate Theses and Dissertations

Melanoma is the deadliest form of skin cancer. Prognosis for early stage melanoma patients is excellent, and surgery is often curative for these patients. However, once patients have presented with invasive disease, the average 5-year survival rate drops significantly from over 90% to between 10 and 15%. Several therapies have been developed to target a commonly mutated oncogene BRAF, or its downstream effectors. Unfortunately, while these treatments show robust initial response, most patients relapse within a year. Moreover, therapy-resistant tumors are often more invasive and metastatic. Therefore, it is important to investigate the molecular mechanisms underlying melanoma invasion and metastasis, …

Behavioral Thermoregulation And Thermal Mismatches Influence Disease Dynamics In Amphibians, Erin Louise Sauer

USF Tampa Graduate Theses and Dissertations

Amphibians are currently the most threatened vertebra taxa on the planet. Hundreds of species are thought to have gone extinct while thousands more have been listed as threatened or endangered over the past few decades. Habitat loss, invasive species, climate change, and disease are all thought to have partially contributed to these declines. Two pathogens in particular, infectious viruses in the genus Ranavirus (simply referred to as ranavirus) and the fungus Batrachochytrium dendrobatidis (Bd), have been associated with global mass mortality events of amphibians. Virulent pathogens such as these tend to impose strong selective pressures on their hosts driving the …

Aqueous Humor Dynamics And The Constant-Pressure Perfusion Model Of Experimental Glaucoma In Brown-Norway Rats, Kayla R. Ficarrotta

USF Tampa Graduate Theses and Dissertations

Glaucoma affects tens of millions of people and is the leading cause of irreversible blindness worldwide. Virtually all current glaucoma therapies target elevated intraocular pressure (IOP); however, the contribution of intracranial pressure (ICP) to glaucoma has recently garnered interest. Strain at the optic nerve head is now known to depend on the translaminar pressure difference (TLPD), which is the difference between IOP and ICP, rather than IOP alone. A better understanding of how IOP and ICP relate to glaucoma development and progression is essential for developing improved therapies and diagnostic tests. Glaucoma is commonly modeled in rats, yet aqueous humor …

Students’ Opportunity To Learn Surface Area And Volume In Middle Grades Mathematics Textbooks, Sofia Hatziminadakis

USF Tampa Graduate Theses and Dissertations

I conducted a content analysis to examine the treatment of the surface area and volume concepts within four published middle-grades mathematics textbook series. In particular, I examined the treatment of the surface area and volume concepts in terms of the location of surface area and volume lessons in the textbook and the number of pages and lessons devoted to these concepts. I also investigated the sequence of the instructional blocks of surface area and volume lessons. In addition, I evaluated the tasks included in these lessons in regards to the performance expectations of students, the types of visual representations of …

Investigating The Role Of The Rna Binding Protein Lin28 In The Human Placenta: Implications For Preeclampsia, John Canfield

USF Tampa Graduate Theses and Dissertations

An essential event during early pregnancy is the invasion of trophoblasts into the maternal decidua, which is necessary for proper implantation and establishment of maternal-fetal interface and ultimately allows for proper nutrient exchange and immunological tolerance of the growing fetus. For this invasion to occur, cells originating from the trophectoderm undergo an epithelial to mesenchymal transition to become invasive extravillous trophoblasts and begin invading the uterine decidual tissue. Through the secretion of matrix metalloproteinases and through interactions with many cytokines and cell-adhesion molecules, this well-orchestrated process of trophoblast invasion results in extensive remodeling of the maternal spiral vasculature by the …

The Unmanned Aerial Systems (Uass) Industry And The Business Impacts Of The Evolution Of The Federal Regulatory Environment, Darren W. Spencer

USF Tampa Graduate Theses and Dissertations

Despite the explosion of popularity of UASs, and the recognition that such systems must find a way to safely operate alongside manned aviation, a literature review by this author as well as interviews with three commercial aerial photography companies in Tampa Bay, Florida, indicate that regulatory restrictions are still the greatest obstacle to law abiding commercial UAS operators. It can take six to eight months with a backlog of 12,000 waiver applications to get either a Part 333 or Part 107 exemption, which grants FAA permission for a commercial operator to fly a UAS inside controlled airspace (Gardner, 2018). A …

A Secure Computing Platform For Building Automation Using Microkernel-Based Operating Systems, Xiaolong Wang

USF Tampa Graduate Theses and Dissertations

Building Automation System (BAS) is a complex distributed control system that is widely deployed in commercial, residential, industrial buildings for monitoring and controlling mechanical/electrical equipment. Through increasing industrial and technological advances, the control components of BAS are becoming increasingly interconnected. Along with potential benefits, integration also introduces new attack vectors, which tremendous increases safety and security risks in the control system. Historically, BAS lacks security design and relies on physical isolation and "security through obscurity". These methods are unacceptable with the "smart building" technologies. The industry needs to reevaluate the safety and security of the current building automation system, and …

Negotiating My Rainbow: A Self-Study Exploring The Relationship Between Beliefs About Identity And Classroom Practice, Amanda Colborne

USF Tampa Graduate Theses and Dissertations

Teachers bring parts of themselves, among them, gender, age, and race into the classroom. In addition to the routine stress of teaching, lesbian, gay, bisexual and transgender (LGBT) people have the added stress of managing the expression of their sexual orientation in the classroom. This study explores the ways in which my own identity as a lesbian influenced my beliefs about teaching, my “pre-active” curricular-instructional decision-making, and my “interactive” curricular-instructional decision-making (Jackson, 1966). A self-study methodology is used to explore these relationships. Data sources include journaling, lesson plan artifacts, student work samples, photographs of my classroom, an observation and critical …

The Icarus Floating Membrane Photobioreactor For Microalgae Cultivation In Wastewater: Advancing Technology From Lab To Field Prototype, Melanie Pickett

USF Tampa Graduate Theses and Dissertations

The ICARUS membrane photobioreactor, conceptualized and preliminarily tested at the University of South Florida, provides a viable platform for algae cultivation using wastewater as a growth medium. Progression of the technology is tracked using NASA’s Technology Readiness Level scale, and is herein described. Through the work described in this manuscript, the technology has evolved from TRL 3 to TRL 5. Completing the suggested future work will readily move the technology to TRL 6 and beyond.

The first study investigated the co-location of algal cultivation with wastewater treatment. This coupling provides a steady source of water and nutrients for algal growth. …

Validation Of A New Concept For Measuring Respirable Dusts, Xiao Liu

USF Tampa Graduate Theses and Dissertations

Sampling of airborne dust in industry is influenced by the potential health effects associated with human exposure. Health effects depend on mass concentration and particle size which influences the site of pulmonary deposition. Occupational diseases tend to be associated with dust deposition in specific regions of the respiratory tract. The ACGIH size selective TLVs are expressed in three forms: Inhalable Particulate Matter, Thoracic Particulate Matter, and Respirable Particulate Matter. In pneuomoconioses, the amount of dust deposited in the lungs can be estimated by sampling the respirable fraction. Dose-response relationships are derived by relating the health experience of workers to the …

The Politics Of Medicine At The Late Medici Court: The Recipe Collection Of Anna Maria Luisa De’ Medici (1667 – 1743), Ashley Lynn Buchanan

USF Tampa Graduate Theses and Dissertations

This dissertation analyzes the social, cultural, and political significance of recipes at the late Medici court. In doing so, it examines how the late Medici court used medicinal and pharmaceutical patronage to maneuver politically and socially as well as increase the court’s cultural cache throughout Europe. By the beginning of the eighteenth century, it was clear that the Medici line would end and that the Grand Duchy of Tuscany would become a satellite state of a larger European power. Yet while the late Medici court found themselves increasingly sidelined in the cultural and political landscape of Europe, science and medicine …

Quantifying The Probability Of Lethal Injury To Florida Manatees Given Characteristics Of Collision Events., B. Lynn Combs

USF Tampa Graduate Theses and Dissertations

Wherever wildlife share space with boaters, collisions are a potential source of mortality. Establishing protection and speed zones are the primary actions taken to mitigate collision risk. However, creation of protection zones may be a point of contention with stakeholders as new zones can have significant socioeconomic impacts. The Florida Manatee is a prime example of a species whose abundance and viability are constrained by this balance between the needs of humans and wildlife on a shared landscape. The goal of this work is to help further understand the risk to manatees by quantifying the probability of lethal collisions. I …

Evaluating The Use Of Larval Connectivity Information In Fisheries Models And Management In The Gulf Of Mexico, Michael Drexler

USF Tampa Graduate Theses and Dissertations

Connectivity is a major contributor to the overall dynamics of marine populations. However, it still remains challenging to describe connectivity on ecologically meaningful scales of time and space. This is a major impediment to evaluating the impacts of marine protected area with respect to fisheries management objectives.

This dissertation brings together a wide array of spatial and connectivity information in the Gulf of Mexico (GOM) with the goal of 1) understanding the spatial distribution of fish populations and source-sink dynamics and 2) evaluating whether this information can be integrated, through a modeling framework, to identify closed areas that could be …

Use Of A Towed Camera System Along The West Florida Shelf: A Case Study Of The Florida Middle Grounds Benthic Marine Communities, Katie S. Davis

USF Tampa Graduate Theses and Dissertations

As technologies advance the study of ocean dynamics, new approaches to vexing problems of scale and process are becoming more widely available. Originally conceived as a tool primarily for indexing the abundance of near-bottom fishes, the Camera-based Assessment and Survey System (C-BASS) may also be an effective tool for monitoring benthic invertebrate resources vulnerable to natural and anthropogenic perturbations, and for characterizing the composition of benthic communities to inform spatial management. Using still images derived from the C-BASS video of benthic transects within the Florida Middle Grounds, I documented the abundance of benthic habitat-forming functional groups—sponges, algae, and corals—and noted …

Enhanced Co2 Conversion By The Simultaneous Doping Of Co, Fe, And Mn In The B-Site Of A Lanthanum Perovskite, Adela E. Ramos

USF Tampa Graduate Theses and Dissertations

There is a pressing need for carbon dioxide (CO₂) mitigation technologies as increasing emissions continue to threaten our environment. Carbon capture and storage (CCS) alone would not be able to achieve temperature rise below 2 °C. In addition, CCS cost increases considerably for power generation and other industries where CO₂ separation is necessary. Conversion of CO₂ to usable products is the most economical and feasible solution as it could offset CO₂ separation cost. Liquid fuels are an attractive route since it is a commodity used globally and demand increases with population and economic growth. Conversion …

Evaluating Human-Associated Escherichia Coli Marker Genes For Microbial Source Tracking Applications In Florida, Jacob K. Senkbeil

USF Tampa Graduate Theses and Dissertations

Fecal pollution of surface water can lead to human health issues because waterborne transmission of pathogens is a common cause of gastroenteritis. Genetic markers developed for human-associated Escherichia coli (H8, H12, H14, and H24) are promising tools for microbial source tracking (MST) of sewage in environmental waterbodies but are untested in the US. H gene marker performance characteristics (specificity, sensitivity, and prevalence) were assessed, with the goal of developing a quantitative PCR assay for the markers with the best performance. Individual E. coli isolates from reference samples were tested to determine end-point PCR assay performance. Three hundred E. coli strains …