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An Investigation Into The Protein Dynamics And Proton Transfer Mechanism Of Class-A Β-Lactamase (Ctx-Ms) By Nmr Spectroscopy, Radwan Ebna Noor

USF Tampa Graduate Theses and Dissertations

Extended-spectrum β-lactamases (ESBLs) of different Enterobacteriaceae (e.g. Escherichia coli, Klebsiella pneumoniae) have developed significant resistance mechanisms against antibiotics. ESBLs, like class A CTX-Ms (cefotaximases) enzymes hydrolyze the β-lactam ring of different antibiotics such as penicillins and third-generation oxyimino-cephalosporins. Although CTX-Ms are repeatedly found in many clinical and community settings, molecular-level information on their function/activity is currently limited to structural studies performed by X-ray crystallography. In order to gain further insights into the structure and functions of this class of proteins, it is also essential to fully understand their dynamic and chemical properties in solution. In order to better understand the …

Desertification Risk Analysis For Wildlife In The Desert Renewable Energy Conversation Plan Area Of California, Serdar Sagdic

USF Tampa Graduate Theses and Dissertations

Desertification is one of the primary threats facing the sustainability of the Earths’s natural resources. Desertification it is an outcome of human impacts to ecosystems and alterations of climate patterns resulting in land degradation and adverse effect on natural resources, vegetation loss, soil infertility, and water scarcity. These changes influence the existence and distribution of wildlife by reducing the availability and quality of habitat that provides food, water, cover, and other life requisites. Therefore, this study aimed at evaluation of potential desertification risks on wildlife species in the Desert Renewable Energy and Conservation Plan (DRECP) area of California.

The DRECP …

Implementing The Break Pass Intervention With Differential Reinforcement To Improve Engagement In Play With Siblings For Children With Autism Spectrum Disorder, Shannon R. Shafmaster

USF Tampa Graduate Theses and Dissertations

This report describes the results from a study that examined the use of the break pass intervention to increase engagement in play with siblings for children with autism spectrum disorder. Two male children with ASD aged 6-years old, who had difficulty engaging in play activities with siblings participated in the study. The intervention used break passes to teach mands for breaks by providing the children with breaks throughout the play activity and to provide reinforcement for engaging in play with siblings in the absence of problem behavior. The impact of the intervention on engagement in play with siblings and disruptive …

Bioactivity Of Suberitenones A And B, Jared G. Waters

USF Tampa Graduate Theses and Dissertations

Throughout human history, natural products have formed the foundation of medicine. In ancient times, a myriad of herbs and fungi have been attributed properties of healing and rejuvenation. Today, that foundation still very much exists, as natural product chemists isolate bioactive compounds from plants, fungi, animals, and microorganisms alike. As more of these secondary metabolites are discovered, scientists pursue more and more sources of biodiversity that may yield new and unprecedented compounds. To this end, Antarctica has become of particular note, as its unique environmental conditions and highly isolated nature make for rare and unusual adaptations in its native wildlife. …

Applied Anthropology Of Addiction In Clinical Spaces: Co-Developing And Assessing A Novel Opioid Treatment Pathway, Heather Diane Henderson

USF Tampa Graduate Theses and Dissertations

Historically, there has been no clear treatment pathway for opioid use disorder (OUD) patients who seek emergency care. Utilizing a trajectories of addiction framework, this dissertation presents insights from an applied anthropology of addiction study in an acute care setting to build a medication for opioid use disorder (MOUD) treatment pathway, aiming to break down the stigma related to opioid use disorder (OUD) and mitigate a lack of treatment options. This dissertation took place in an urban trauma center in Florida, from 2018 to 2021. With almost 1500 hours of participant observation, hundreds of informal interviews, and 30 formal interviews, …

Empirical And Modeled Δ13c And Δ15n Isoscapes In The Gulf Of Mexico And Their Application To Fish Eye Lens Migration Studies, Brianna Michaud

USF Tampa Graduate Theses and Dissertations

Isoscapes are depictions of the spatial patterns of isotopic values in a given area. Isoscapes can be created using measurements form samples (empirical isoscapes) or using statistical models of spatial isotopic variation (modeled isoscapes). Isoscapes have a wide variety of potential applications though, in the realm of marine ecology, they are most often used infer ecological processes, food web linkages, the origin of samples, and the movements of marine organisms.

However, to use isoscapes for these applications, it is necessary to have isoscapes at spatial scales relevant to the application in question. It is also necessary to have isoscapes that …

Understanding Food Insecurity As A Multidimensional Construct: A Mixed Methods Approach To Assess Changes In Food Insecurity Status Among People Seeking Emergency Food Assistance, Whitney M. Fung Uy

USF Tampa Graduate Theses and Dissertations

Background: In the United States, 13.8 million households were food insecure in 2020, or 10.5% of the country’s population. Although the annual food insecurity rates remained stable between 2019 and 2020, largely due to increased COVID-19 pandemic relief efforts, many households experienced food insecurity and hardship at unprecedented rates. Food assistance provided by the largest government nutrition and food program, Supplemental Nutrition Assistance Program (SNAP), and emergency food providers (EFPs) such as food banks and pantries intended to provide short-term solutions have ended up as chronic, long-term methods of survival for many of these families, especially during COVID-19 and the …

Noninvasive In Vivo Optoacoustic Imaging Of Alcohol Use Disorder Using A Mouse Model Of Alcohol Use Disorder, Augustine Meombe Mbolle

USF Tampa Graduate Theses and Dissertations

This research has two parts. The first part focuses on the use of photoacoustic imaging (PAI) to study alcohol-induced effects in the cerebral vasculature of selectively bred alcohol-preferring mice. Part two extends the application of PAI by developing a novel Photoacoustic imaging guided system for repetitive low intensity focused ultrasound (PAI-LIFU) stimulation treatment for alcohol use disorder in crossed high alcohol preferring (cHAP) mice.

Photoacoustic imaging is an emerging hybrid non-invasive optical imaging modality which relies on optical absorption contrast to visualize deep tissue structures and function. It combines the high contrast associated with pure optical imaging and the high …

Finding Signal In The Noise: High-Fidelity, Quantitative, Optical Blood Perfusion Imaging With Interference, Abdul Mohaimen Safi

USF Tampa Graduate Theses and Dissertations

For label-free, non-invasive, wide field-of-view (FOV) imaging/monitoring of blood flow, speckle-based approaches are gaining popularity. However, to obtain quantitative flow information, speckle techniques rely on the multi-exposure scheme which requires complex, bulky, and expensive instrumentation, limiting its application to preclinical studies. This dissertation directly addresses these issues. In the first part of this dissertation, we report a novel single shot synthetic multi-exposure speckle imaging (syMESI) method to synthetically produce multi-exposure images from one short single exposure speckle image using spatial binning/averaging. We demonstrate that syMESI can reimagine conventional hardware based MESI, with low-cost single exposure laser speckle imaging (LSCI) instrumentation. …

Origin And Epigenetic Regulation Of Cutaneous T Cell Lymphoma, Carly M. Harro

USF Tampa Graduate Theses and Dissertations

The pathogenesis of Cutaneous T cell lymphoma (CTCL) remains unclear. Using conditional knockout mice, we found that ablation of the genomic organizer Special AT-rich sequence-binding protein-1 (Satb1) caused malignant transformation of mature, skin-homing, Notch-activated CD4+ and CD8+ T cells into progressively fatal lymphoma. Mechanistically, Satb1 restrained Stat5 phosphorylation and the expression of skin-homing chemokine receptors in mature T cells. H3K27 and H3K9 trimethylation occluded the SATB1 promoter in Sézary cells, while inhibition of SUV39H1/2 methyltransferases (unlike EZH2 inhibition), restored protective SATB1 expression and selectively abrogated the growth of primary Sézary cells more effectively than romidepsin. Additionally, using single-cell RNA/TCR sequencing …

Thermochemical Conversion Of Lipid-Extracted Algal Biomass To Value-Added Sustainable Products, Magdalini Tsarpali

USF Tampa Graduate Theses and Dissertations

The extensive use of fossil fuels has increased the CO2 emissions, a phenomenon that has increased the demand for renewable energy and resources. Algae, photosynthetic aquatic organisms, have recently caught attention due to their high growth rate and the high CO2 capture ability. Biofuels and bioproducts from algae are considered promising alternatives because of their lower carbon footprint. Lipids are by far the most popular algal product, as they find applications in both fuels, such as biodiesel, and products, such as nutraceuticals and cosmetics. However, after lipid extraction, conversion of the residual algal biomass to value-added products is viewed as …

Establishment Of A Melanoma Esc-Gemm Platform And Its Use To Study Pten Tumor Suppressor Functions, Ilah Bok

USF Tampa Graduate Theses and Dissertations

The cumbersome and time-consuming process of generating new mouse strains and multiallelic experimental animals often hinders the use of genetically engineered mouse models (GEMM) in cancer research. In chapter 2, we describe the development and validation of an embryonic stem cell (ESC)-GEMM platform for rapid modeling of melanoma in mice. The platform incorporates 12 clinically relevant genotypes composed of combinations of four driver alleles (LSL-BrafV600E, LSL-NrasQ61R, PtenFlox, and Cdkn2aFlox) and regulatory alleles to spatiotemporally control the perturbation of genes of interest. The ESCs produce high-contribution chimeras, which recapitulate the melanoma phenotypes of conventionally bred mice. Using the ESC-GEMM platform to …

Defining And Modeling Pericyte-Induced Capillary Network Assembly, Capillary Regression Preceding Pericyte-Stimulated Fibrosis, And Lymphatic Capillary Regression, Scott Stephen Kemp

USF Tampa Graduate Theses and Dissertations

Capillary formation and maturation can only occur through a series of highly orchestrated events that requires the coordinated efforts of both endothelial cells (ECs) and pericytes. While ECs are perfectly capable of creating tube networks on their own, the maturation and stabilization of these tube networks require the presence of a second cell type, pericytes, to create stable capillary networks. Previous studies have identified factors required for pericyte recruitment to EC tube networks, however, we hypothesized that there were still unaccounted for EC-derived molecules that were contributing to pericyte recruitment. In the first part of this study, we used serum-free, …

Targeting The Side-Chain Convergence Of Α-Helical Hot Spots To Design Small-Molecule Mimetics Disrupting Protein-Protein Interaction, Zhen Wang

USF Tampa Graduate Theses and Dissertations

α-Helix accounts for the largest class of protein secondary structures and plays a key role in mediating protein-protein interactions (PPIs). Small-molecule α-helix mimetics mimic the structural features of α-helical hot spots and offer a powerful tool to unravel complex signaling networks and mitigate aberrant PPIs. In this dissertation, the side-chain conformational convergence of α-helical hot spots was disclosed, and insights into the side-chain convergence were exploited to instruct the design of small-molecule α-helix mimetics. The first chapter of this dissertation provides an overview of the characteristics of α-helical hot spots and current strategies to design small-molecule α-helix mimetics. In chapter …

Improving Robustness Of Deep Learning Models And Privacy-Preserving Image Denoising, Hadi Zanddizari

USF Tampa Graduate Theses and Dissertations

Applications of deep learning models and convolutional neural networks have been rapidly increased. Although state-of-the-art CNNs provide high accuracy in many applications, recent investigations show that such networks are highly vulnerable to adversarial attacks. The black-box adversarial attack is one type of attack that the attacker does not have any knowledge about the model or the training dataset, but it has some input data set and theirlabels.

In this chapter, we propose a novel approach to generate a black-box attack in a sparse domain, whereas the most critical information of an image can be observed. Our investigation shows that large …

Development Of Enhanced Algae Strains And Sustainable Cultivation Processes For Production Of Algal Lipids For Biofuels And Bioproducts, Enlin Lo

USF Tampa Graduate Theses and Dissertations

The establishment of an algae industry is crucial for addressing global biofuel and bioproduct demand that meets the Sustainable Development Goals (SDG) worldwide. Photosynthetic microalgae are excellent sources for food, fiber, fuel, feed, and fertilizer, which are the 5Fs of the SDG. However, at present, algae-based materials are not cost-competitive or sufficiently sustainable. Improved productivity, lower cultivation cost, and reduced use of resources are required to transition from lab to industry, necessitating the development of superior strains that have high productivity and can tolerate environmental stress, such as temperature and salinity, so they can be cultivated at large scale outdoors …

Exploring The Structure And Activity Of Metallo-Tetracyclines, Shahedul Islam

USF Tampa Graduate Theses and Dissertations

Copper as a key component of electron transport chain of eukaryotes is an essential transition metal ion. Copper homeostasis in mammals complex and tightly regulated. Its strong reactivity together with binding with biologically important chemicals can have important repercussions in human health and overall environment. Tetracycline as one of most abundantly used antibiotic of the world has become abundant in the environment as well. Herein in this dissertation we take the journey to explore the complexation of tetracycline with metals in the environment and the intricate interaction between copper and tetracycline by investigating their oxidative behavior once they are complexed. …

Individual Differences In Response To Hostile And Benevolent Sexism In A Stem Interview Context: The Moderating Role Of Behavioral Activation, Elizabeth Kiebel

USF Tampa Graduate Theses and Dissertations

Women continue to face sexism in workplace contexts, especially those that are male dominated, such as science, technology, engineering, and mathematics (STEM). Yet, women often fail to confront the sexism they experience, despite confrontation being an effective way to cope with and prevent future harassment (Fitzgerald et al., 1995; Magley, 2002). To date, no one has assessed the potential moderating role of personality differences related to approach motivation on women’s confrontation of sexism. In this study, women were exposed to either a hostilely sexist or benevolently sexist question during a mock job interview that was purportedly being conducted as part …

Defining The Role Of Oxidized Mitochondrial Dna In Myelodysplastic Syndromes, Grace Anne Ward

USF Tampa Graduate Theses and Dissertations

Myelodysplastic Syndromes (MDS) are heterogeneous bone marrow (BM) failure malignancies characterized by constitutive innate immune activation, NLRP3 inflammasome (IFM) driven pyroptotic cell death, and the induction of interferon-stimulated genes (ISG). MDS often diagnostically resembles other hematologic disorders, as such, additional tools are needed for the discrimination from overlapping disorders. We observed that oxidized mitochondrial DNA (ox-mtDNA) is released upon cytolysis. We hypothesize that levels of ox-mtDNA are high in MDS, allowing us to use it as a diagnostic biomarker; and that cell free ox-mtDNA is contributing to feedforward BM failure.

ROC/AUC analysis demonstrated that ox-mtDNA is a sensitive and specific …

A Mixed Methods Analysis Of Maternal-Infant Feeding Interactions Between Dyads With Opioid Exposure, Kailey T. Rinaldi

USF Tampa Graduate Theses and Dissertations

Infants diagnosed with neonatal opioid withdrawal syndrome (NOWS) exhibit neurobehavioral dysregulation that may make them challenging to feed, potentially impeding positive early maternal-infant interactions. The goal of this study was to investigate dyadic behavior during the maternal-infant feeding interaction, specifically infant dysregulation and maternal sensitivity, between dyads with opioid exposure in the neonatal intensive care unit (NICU). This was a secondary data analysis using a mixed-methods design to analyze videorecorded feeding interactions of mothers with opioid use disorder (OUD) and their infants with NOWS. An integrative literature review investigating maternal sensitivity in mothers with OUD was conducted. Verbalizations expressed by …

Geothermal Development In Kenya: Gender Based Socio-Cultural And Socio-Economic Implications, Faye N. Ricker

USF Tampa Graduate Theses and Dissertations

This dissertation is an ethnographic case study that analyzes the gendered socio-cultural and socio-economic implications for a community that was displaced in order to develop a geothermal energy system in Kenya. The implications are rendered particularly interesting because the displaced community is also one of the communities served by the geothermal power grid. The analysis employs a composite framework of Feminist Political Ecology and the Political Ecology of Climate Change Mitigation. This work explores gendered perceptions and control of energy, livelihood activities, place attachment, and the cultural/spiritual significance of geothermal energy.

Geothermal energy is a potentially promising source of low-carbon …

A Statistical Method For Quantifying Animal Interaction; Combining Time Geography And Simulation, Zachary J. Smith

USF Tampa Graduate Theses and Dissertations

Dynamic interaction (DI) describes the synchronous interaction of one or more individual animals over time. Current methods to quantify DI from tracking data are lacking. They typically require synchronized tracking data which limits their applicability to most studies. They also often do not have built-in tests of significance to evaluate interaction, and if so, rely on random walk models that lack biological realism. This research aimed to develop and evaluate a new way of measuring animal interaction that overcomes these limitations. The goal was to create a statistical method for quantifying and evaluating interaction that is relevant across computational, behavioural, …

Phenotypic Variability In Ipsc-Induced Cardiomyocytes And Cardiac Fibroblasts Carrying Diverse Lmna Mutations, Jiajia Yang

USF Tampa Graduate Theses and Dissertations

Mutations in the LMNA gene (encoding lamin A/C) are the second most common cause of familial arrhythmogenic cardiomyopathy. Diverse LMNA variants in cardiomyocytes have been associated with cardiac phenotypes. Moreover, cardiac fibroblasts make up a large fraction of the myocardium's non-myocyte component, intrinsically linked to extracellular matrix synthesis and turnover as well as secreting large amounts of bioactive metabolites. However, our understanding of how different mutation sites and the non-myocyte niche mediate cardiomyocyte function is limited. To fill this gap, in this thesis I investigated the hypothesis that variable LMNA mutations have significant effects on the genetic, structure and electrophysiological …

The Role Of Eicosanoid Metabolism In Mammalian Wound Healing And Inflammation, Kenneth D. Maus

USF Tampa Graduate Theses and Dissertations

Inflammatory wounds, both chronic and acute, lead to increased morbidity and mortality rates, especially in the elderly population. The annual healthcare cost for chronic wound care alone is over $39B in the US and the demographic of susceptible patients is steadily increasing due to an aging population and lifestyle-related diseases (e.g., hyperlipidemia, obesity, and type 2 diabetes). In fact, many chronic wounds currently have a worse 5-year outlook than certain types of cancers. This shows the need for expediting the wound healing process in such a way that compresses inflammatory signaling and encourages wound resolution without sacrificing pathogen removal and …

The Labors Of Professional Wrestling: The Dream, The Drive, And Debility, Brooks Oglesby

USF Tampa Graduate Theses and Dissertations

This study centers and examines the laboring body of the professional wrestler as it moves and works through professional wrestling training. I put forth debility as a theoretical framework to naming and understanding the ways that long-term bodily wear and early death becomes an acceptable cost of doing business for the wrestling industry. Dreams of achieving wealth, fame, and stability while escaping precarity and obscurity draw fans toward joining the industry, and these dreams work to maintain a working culture that takes for granted the ways that the wrestler’s body is marked for disposability, wearing down, and early death. I …

An Internet Of Medical Things (Iomt) Approach For Remote Assessment Of Head And Neck Cancer Patients, Ruchitha Chinthala

USF Tampa Graduate Theses and Dissertations

Internet-of-Medical-Things (IoMT) allows for a smart healthcare system to remotely monitor and assess patient’s progress at home. Head and neck cancers (HNC) are treated with various treatment options which are associated with significant side effects, mainly shoulder dysfunction, and trismus (spasm of jaw muscles). However, measurement of patient’s progress, and side effects while undergoing treatment, is limited to evaluation received based on scheduled appointments. Development of strategies to enhance monitoring during follow-up period is needed for earlier identification of problems such as trismus and shoulder dysfunction. In this interdisciplinary research, for the first time, we develop an IoMT enabling application, …

Formation Of Trihalomethanes During Chlorination At South Cross Bayou Advanced Water Reclamation Facility, Pinellas County, Florida, Ian Grabo

USF Tampa Graduate Theses and Dissertations

South Cross Bayou Advanced Water Reclamation Facility (SCB) in Pinellas County, Florida, recently had a spike in effluent concentrations of bromodichloromethane (BDCM) and dibromochloromethane (DBCM) above the regulated limits, due to relying fully on chlorination instead of a combination of chlorination and ultraviolet light. The objective of this study was to determine the operating parameters most strongly affecting the formation of THMs. This was completed by analysis of long-term data sets obtained from SCB by multiple linear regression to create models describing THM formation. In addition, sampling of the chlorine contact chamber (CCC) was done to determine the effects of …

Oyster Reef-To-Mangrove Regime Shifts Along The West Coast Of Florida, Stephen Gregory Hesterberg

USF Tampa Graduate Theses and Dissertations

Ecological regime shifts are transitions in ecosystem state, which can occur either when external conditions gradually change or when large perturbations force a system into an alternative state. Such transitions can exhibit non-linear dynamics, where small changes in external conditions can result in abrupt transition and often occur with little warning. After crossing a tipping point, the system may be unable to revert to its original state if the new regime possesses strong, reinforcing feedbacks. Numerous marine ecosystems have experienced regime shifts due to a combination of stressors that have eroded ecological resilience, such as overfishing, nutrient loading, and climate …

Stitched Together: What We Learn From Secret Stories In Public Media, Sara D. Rocks

USF Tampa Graduate Theses and Dissertations

Where do you go to divulge your deepest secrets? Some may turn to journals and diaries; some turn to family and friends; some turn to religious confessionals; some turn to Twitter; and still, some take their secrets to the grave. There is at least one other way people share their secrets that has thus far received limited scholarly attention: the crowdsourced confessional. In the new media landscape, intimate stories and personal disclosures with strangers can be (and are) produced as content for a vast pool of additional strangers to consume as media. Yet, little is known about the personal narratives …

Visions Of Entanglement And Escape: In-Visible Voice In The Films Of Terrence Malick And George Lucas, Michael Lee Taber

USF Tampa Graduate Theses and Dissertations

This thesis juxtaposes the unexpectedly parallel careers of the filmmakers George Lucas and Terrence Malick. Both popularly and academically, Lucas and Malick are typically conceived as divergent practitioners and are framed in oppositional terms. The goal of this thesis is – in part – to break down this oppositional understanding of Lucas and Malick as filmmakers. This traditional understanding of Malick and Lucas collapses when we consider their respective relationships to sensation and abstraction. Affirmations of sensation and Malick abound, as do critiques of abstraction and Lucas – all the while it is Malick who is aligned with abstraction and …