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An Anthropology With Human Waste Management: Non-Humans, The State, And Matters Of Care On The Placencia Peninsula, Belize, William Alex Webb

USF Tampa Graduate Theses and Dissertations

The management of human waste is a seldom studied phenomenon in anthropology. Yet across the globe, in countries both rich and poor, it presents pervasive and difficult to tame problems. This dissertation draws on complimentary theories of management and entanglements to explore the practices and processes of organizing human waste on the Placencia Peninsula, Belize. The results illustrate how problems are conditioned and defined by messy relations between institutions, people, technologies, materials, and ecological life.

Fieldwork and analysis for this work was a culmination of years of interdisciplinary collaboration between other anthropologists and engineers at the University of South Florida. …

Anthropogenic Fecal Pollution Affects Microbial Water Quality And Multidrug Resistance In Tropical Aquatic Environments, Adriana González-Fernández

USF Tampa Graduate Theses and Dissertations

Although an often-overlooked issue, fecal pollution at recreational beaches should concerneveryone. Worldwide more than 80% of wastewater is discharged into rivers or the ocean without any treatment. Untreated and inefficiently treated wastewater can introduce diseasecausing microorganisms into the aquatic environment. Fecal-borne pathogens can also originate in animal waste, and humans can be exposed to these pathogens from poorly managed animal feces that make their way into the aquatic environment. Exposure to waterborne pathogens in recreational waters is a public health hazard, as it facilitates the transmission of waterborne illness, and surface waters serve as natural reservoirs of antibiotic resistant bacteria …

The Biological Roles Of Tau Protein In The Vasculature And The Regulation Of Vegfr1 Signaling By Heparan Sulfate, Yanan Zhu

USF Tampa Graduate Theses and Dissertations

My dissertation consists of two research projects. The first project investigated the roles of extracellular tau in the vasculature, including tau-induced angiogenesis and tau internalization, a topic related to tauopathy. The second project was to understand the regulatory functions of heparan sulfate proteoglycans in VEGFR1 signaling.

Tauopathies are a class of neurodegenerative diseases, including Alzheimer's disease, and are characterized by the appearance of intraneuronal tau inclusion in the brain and the patient's cognitive decline. Recent studies have emerged that soluble and aggregated tau also exists outside neurons in the central nervous system. Intriguingly, overexpressing tau in neurons in the mouse …

American Horse Power During The Great War, Hanna K. Lipsey

USF Tampa Graduate Theses and Dissertations

This dissertation charts the significant, if understudied, history of American horses during the era of World War I, from roughly 1914 to 1919. Its chapters trace how the US Army acquired, used, cared for, and ultimately demobilized horses over the course of that conflict. Beginning with their acquisition, via either an Army Horse Breeding Program or a complicated buying process, horses faced a complex introduction into military service. Life for these animals did not get any easier once they reached the European front. Although the US military was beginning to replace horses with motor trucks and tractors, horses remained central …

Beyond Health And Animal Rights: A Study In Black Veganism, Wendy J. Rib

USF Tampa Graduate Theses and Dissertations

Veganism, or the avoidance of animal products, has been recognized by nutritionists and food scientists as a lifestyle choice associated with good health and lower prevalence of diabetes, obesity, hypertension, and heart disease. However, the choice to eschew all animal products is frequently more than just a diet. Many people practice veganism as part of a larger reformative social movement, seeking permanent change in food structures, advancing policies in animal welfare and transforming their everyday lives in the quest for cultural acceptance. Although the number of people who identify as vegans appears to be growing, it is often assumed that …

Farmers’ Organizations And Development Actors In A Pandemic: Responses To Covid-19 And The Food-Energy-Water Nexus, Atte Penttilä

USF Tampa Graduate Theses and Dissertations

The Covid-19 pandemic disrupted the lives of millions, if not billions, in some shape or form. This global multi-sited dissertation documents responses and changes in the agricultural development context within the AgriCord Alliance and network as they had appeared during the pandemic, with a specific focus on co-production of resilience and the Food-Energy-Water Nexus. The research elucidates experiences representing the whole ‘food chain’ of a global agricultural development network. As the Covid-19 pandemic was a global event, it offered an opportunity for global disaster research. During the pandemic, the farmers’ organizations and their members faced economic stress as marketplaces closed …

Health Effects Of Oil Spills And Dispersal Of Oil Droplets And Zooplankton By Langmuir Cells, Sanjib Gurung

USF Tampa Graduate Theses and Dissertations

This Ph.D. dissertation deals with the study of the health effects of crude oil vapors from oil spills, the effect of Langmuir circulation on the dispersion of oil droplets during oil spill events, and the biological significance of Langmuir circulation. The introduction to areas of three major research projects undertaken during my Ph.D. is included in chapter 1 and the relevant literature review is included in chapter 2. The first research project described in chapter 3 involves the quantification of health effects on embryonic Gulf killifish due to exposure to crude oil vapors. The second research project described in chapter …

Assessment And Prevention Of Bacterial Regrowth In Stored Household Water In Eastern Coastal Madagascar, Lauren Judah

USF Tampa Graduate Theses and Dissertations

As of 2020, diarrheal diseases were the leading cause of death in Madagascar. A major cause of diarrheal illness is contaminated water, and one crucial aspect of preventing contaminated water is safe household storage. Bacterial regrowth between point-of-collection and household storage has been well established in literature. A frequently implicated source of regrowth is the storage container’s biofilm, which is a community of varying bacteria enclosed in a “slimy” extracellular polymeric substance that forms on surfaces in wet environments and anchors bacteria to those surfaces. The overall goal of this thesis was to quantify and control bacterial regrowth in household …

Using Online Reviews To Identify How Hotels Can Satisfy Travelers With Pets While Making Money, Sonia Weinhaus

USF Tampa Graduate Theses and Dissertations

A Qualitative Content Analysis of user generated content in the form of online reviews reveals an opportunity for hotels to better serve Travelers With Pets and collect partitioned fees. Examining more than 3,000 reviews of 92 hotels from five geographical regions of the United States identifies how to close the gap between what customers want and what hotels offer. This study derives the Minimum and Maximum Typologies of pet policy structures from the coding of online reviews. Travelers With Pets are willing to pay partitioned fees if the fee is viewed as fair. Improving saliency of pet fees is revenue …

Utilizing Maternal Investment Strategies Of Sharks To Develop Management Tools To Aid In Shark Conservation, Dennis Deeken

USF Tampa Graduate Theses and Dissertations

Every generation, breeding females and males repopulate ecosystems with the next generation of offspring. Two models link maternal investments in offspring size and number to natural selection processes. In unstable resource environments, bet-hedging models predict a conservative tradeoff in maternal investments resulting in moderate egg size and clutch size within a given range of sizes for a given species. r/K selection models make predictions on maternal investments across species. In unstable resource environments, r-selected species invest in large broods of small offspring. In stable environments, K-selected species invest in small broods of large offspring. The maternal risk management model predicts …

To Be Or Not To Be: A Tale Of Staphylococcal Gpsb, Lauren R. Hammond

USF Tampa Graduate Theses and Dissertations

There are two big drivers motivating studies into bacterial cell division. The first is a desire to understand life around us. One of the defining characteristics of life is the ability for a cell to grow and divide, and without in-depth knowledge of this process, we cannot truly understand the complexities that allow for life on this planet. The second motivator is to identify new drug targets in the ongoing fight against antimicrobial resistance. Many infectious organisms have become resistant to commonly used antibiotics, and the bacterial cell division machinery is a largely untapped essential process with many potential therapeutic …

Design And Assessment Of A Novel Edna Survey Method For The Eastern Indigo Snake (Drymarchon Couperi), Emily K. Galbraith

USF Tampa Graduate Theses and Dissertations

1. Accurate information about species range declines is the cornerstone of effective conservation when habitat loss drives biodiversity loss. The Eastern Indigo snake (Drymarchon couperi) is an apex predator in Florida and, like many other native Florida species, is threatened by widespread habitat destruction. Environmental DNA (eDNA) monitoring of this elusive snake would provide a low-risk and low-cost approach to expand our knowledge of the species’ range and distribution.

2. We designed and tested an eDNA assay that can detect the presence of Eastern Indigo snake from soil samples of their natural habitat. This assay was …

Effect Of Hyperoxia On Cardiac Pathophysiology In Female Guinea Pig Hearts, Chayapatou Chayawatto

USF Tampa Graduate Theses and Dissertations

Hyperoxia is widely implemented in critical care and ICU patients. The administration of a high concentration of inspired oxygen to the lung can unknowingly cause hyperoxia and thereby damaging the lungs and heart due to oxidative stress. Technically, hyperoxia occurs when the patient receives PaO2 > 200 mmHg. Major research focused on hyperoxia-induced lung injury, but nothing is known on its effect on heart. Our lab is the pioneer in understanding the effect of hyperoxia on cardiovascular remodeling using mice model. Previous results show that mice under 72 hours of hyperoxia present severe cardiac pathophysiology. This research uses Guinea pigs, which …

Interactions Between Juvenile Estuary-Dependent Fishes And Microalgal Dynamics, Ian C. Williams

USF Tampa Graduate Theses and Dissertations

The ontogenetic movement of juvenile estuary-dependent fishes upstream in estuarine settings is a phenomenon observed around the world. These movements usually coincide with a shift in diet from smaller prey, such as calanoid copepods, to larger prey, such as mysids. However, the mechanism that drives this pattern has yet to be conclusively described. Prior to the current study, zooplankton/hyperbenthos (“zooplankton”), primary production, and water quality data were collected from the Caloosahatchee River estuary in two concurrent and coordinated studies over a two-year period. One of the products of these sampling efforts was a classification of primary-producer types at the sediment-water …

Ec Expression Of Activated K-Ras Leads To Capillary Deficiency Due To Excessive Lumen Formation, Reduced Pericyte Recruitment, And An Increased Susceptibility To Mmp-1-Dependent Vascular Regression, Zheying Sun

USF Tampa Graduate Theses and Dissertations

Activated k-Ras mutations play a role in a variety of pathological conditions, such as arteriovenous malformations (AVMs). To study the effect of activated k-Ras mutation on lumen formation, we engineered human ECs to simultaneously express the activated k-Ras (G12V) mutation and compared their ability to form lumens with control ECs. We demonstrated that ECs with k-RasV12 lead to marked stimulation of EC lumen formation due to dramatic increases in intracellular vacuole formation and fusion, signaling changes including accelerated Src kinase activation, and MT1-MMP-dependent lumen expansion, all of which drive the process of lumen formation. The markedly accentuated lumen formation is …

Ambient Athleticism: Politicizing Akira’S Accelerationist Olympiad, Thomas G. Chaplin

USF Tampa Graduate Theses and Dissertations

This thesis politicizes Katsuhiro Otomo’s 1988 animated cyberpunk film, Akira, specifically through how it stages its myriad neoliberal crises as opportunities for accelerationist solutions mediated by the Tokyo Olympics. Akira’s display of fully animated and intense action physics produces an aesthetic relation to its own athletes that contracts around their bodies in an attempt to transgress classical and oppressive compositions. Akira’s vague utopic promise receives broad acceptance and affirmation by extant scholarship, often relying on the accelerative impulses found in the works of Gilles Deleuze to substantiate Akira’s hopeful ending. Invoking Gilles Deleuze’s notion of athleticism, this thesis critically rereads …

Accelerating Multiparametric Mri For Adaptive Radiotherapy, Shraddha Pandey

USF Tampa Graduate Theses and Dissertations

MR guided Radiotherapy (MRgRT) marks an important paradigm shift in the field of radiotherapy. Superior tissue contrast of MRI offers better visualization of the abnormal lesions, as a result precise radiation dose delivery is possible. In case of online treatment planning, MRgRT offers better control of intratumoral motion and quick adaptation to changes in the gross tumor volume. Nonetheless, the MRgRT process flow does suffer from some challenges that limit its clinical usability. The primary aspects of MRgRT workflow are MRI acquisition, tumor delineation, dose map prediction and administering treatment. It is estimated that the acquisition of MRI takes around …

Association Between Use Of Remdesivir And Bradycardia, Gibret Umeukeje

USF Tampa Graduate Theses and Dissertations

Remdesivir received the first emergency use authorization from the FDA for the treatment of COVID-19. Multiple adverse drug reactions (ADR) have been reported since its approval in October 2020. Bradycardia, defined by a decrease in heart rate has been reported as an adverse event for patients receiving remdesivir for COVID-19 treatment. The purpose of the research is to systematically investigate the frequency of occurrence of bradycardia in adults receiving remdesivir using clinical data derived from the FDA Adverse Event Reporting System (FAERS) database. Patients receiving remdesivir were compared to those receiving Paxlovid, Regen-Cov, and Dexamethasone for COVID-19 treatment to see …

Queering The Weeki Wachee Mermaid And Its Renewed Aesthetic Value, Jacqueline D. Merveille

USF Tampa Graduate Theses and Dissertations

Scholars have asserted that the visual representation of womanhood responds to a cultural context, which often adheres to patriarchy and the satisfaction of the male gaze. The mediated depiction of the mermaid through time is polysemous and therefore deserves a thorough examination substantiated by robust theories. As a gendered feminine figure that complies with the patriarchal pressure of subjugation to gratify male desire, the mermaid can, however, elude the socially constructed dichotomy of genders, contributing to her agency and queerness. By investigating the Florida roadside attraction of Weeki Wachee Springs’ mermaid shows and other mediated visual depictions of the sea …

Adaptive Multi-Scale Place Cell Representations And Replay For Spatial Navigation And Learning In Autonomous Robots, Pablo Scleidorovich

USF Tampa Graduate Theses and Dissertations

Place cells are one of the most widely studied neurons thought to play a vital role in spatial cognition. Extensive studies show that their activity in the rodent hippocampus is highly correlated with the animal’s spatial location, forming “place fields” of smaller sizes near the dorsal pole and larger sizes near the ventral pole. Despite advances, it is yet unclear how this multi-scale representation enables navigation in complex environments.

In this dissertation, we analyze the place cell representation from a computational point of view, evaluating how multi-scale place fields impact navigation in large and cluttered environments. The objectives are to …

A Holistic Investigation Of Acidosis In Breast Cancer, Bryce Ordway

USF Tampa Graduate Theses and Dissertations

In the early 20th century, Nobel laureate Otto Warburg made the observation that cells of a carcinoma had considerably higher glycolytic metabolism and considerably lower oxidative metabolism compared to cells of a normal tissue. He postulated that within this observation was the key to deciphering the differences between malignant and normal tissue. It is now well established that tumors of the breast are unequivocally acidic, caused by an abnormal amount of aerobic glycolysis, colloquially known as the Warburg effect. Over the last decades, our group, led by Dr. Robert J. Gillies, has set out to characterize the causes and consequences …

The Impact Of Cannabis On Motivational Processes For Smoked Tobacco And Cigarettes, Claire M. Gorey

USF Tampa Graduate Theses and Dissertations

Cannabis and tobacco are very commonly used across the United States. Given the high prevalence of their dual use, an important health consequence of cannabis is its impact on tobacco usage rates. After all, cigarettes are a leading cause of death in the United States. Theoretical models of drug abuse suggest that cannabis cues and intoxication may be responsible for enhanced smoked tobacco/cigarette motivation. Therefore, the present research sought to assess these routes via two studies. In study 1 (validation & cross-drug cue effects), researchers validated a set of realistic cannabis images among a sample of participants residing in the …

X-Linked Ubiquitin-Specific Peptidase 11 Increases Tauopathy Vulnerability In Women, Yan Yan

USF Tampa Graduate Theses and Dissertations

Women experience significantly higher tau burden and increased risk for Alzheimer’s disease (AD) than men, yet the underlying mechanism for this vulnerability has not been explained. Here, we demonstrate through in vitro and in vivo models, as well as human AD brain tissue, that X-linked ubiquitin specific peptidase 11 (USP11) augments pathological tau aggregation via tau deubiquitination initiated at lysine-281. Removal of ubiquitin provides access for enzymatic tau acetylation at lysines 281 and 274. USP11 escapes complete X-inactivation, and female mice and people both exhibit higher USP11 levels than males. Genetic elimination of usp11 in a tauopathy mouse model preferentially …

Chemical Investigation And Drug Discovery Potential Of Terpenoid Secondary Metabolites From Three Deep-Sea Irish Soft Corals, Joshua Thomas Welsch

USF Tampa Graduate Theses and Dissertations

Secondary metabolites produced primarily by terrestrial plants and animals have provided the basis for treatments and cures of human diseases as far back as historians have been able to probe. However, recent technological advances including the self-contained underwater breathing apparatus, and even further remotely operated vehicles, have allowed researchers a unique opportunity for targeted collection of marine organisms at depths previously thought to possess conditions unsustainable for life as was understood. This has afforded a glimpse into the potential for not only life, but the potential for new biochemical adaptations developed over billions of years that have been evolutionarily developed …

4d Printing Of Smart Hydrogel Scaffold To Program Neural Stem Cell Differentiation, Omar Khater

USF Tampa Graduate Theses and Dissertations

Injuries like Traumatic brain injuries (TBI) and Spinal cord injuries (CSI) areconsidered one of the biggest challenges in the medical sector as there is no specific treatment for these injuries and the medical intervention on these injuries is restricted to reducing the pain and removing the damaged human parts. One of the helpful and most effective treatment methods for these injuries involve neural tissue regeneration in the area of the injury. The main issue when trying to treat such injuries is that neural tissues are often unable to regenerate by themselves and require outer human intervention to differentiate. Biomedical scaffolds …

Elemental Sulfur And Metal Sulfide Minerals For Autotrophic Denitrification: Applications To Aquaculture, Groundwater Treatment, And Domestic Wastewater Treatment, Erica Amah Dasi

USF Tampa Graduate Theses and Dissertations

Nutrient pollution of waterways, from excess N and P, is a global concern that results in harmful impacts the environment, human health, and aquatic animals. This dissertation harnesses sulfur autotrophic denitrification to remove nutrients from groundwater, domestic wastewater, and wastewaters produced from marine recirculating aquaculture systems (RAS). Part 1 evaluates the denitrification performance of woodchips and elemental sulfur in cyclic denitrification filters (WS-CDFs) integrated into marine RAS operated under saline conditions. Part 2 investigates the use of metal sulfide minerals, with and without oyster shells, for supporting nutrient removal from groundwater in batch reactors. Part 3 assesses …

Functional Morphology And Taphonomy Of Ordovician–Silurian Diploporan Echinoderms, Stephen Ashley Hill

USF Tampa Graduate Theses and Dissertations

Diploporan echinoderms were one of the most speciose groups of stemmed echinoderms during the Ordovician. Despite this widespread abundance, the fossil record of diploporan fauna consists primarily of incomplete specimens, with complete specimens being rare. While the theca, or body, is more commonly preserved, the feeding appendages and the attachment structures (i.e. stems or holdfasts) are seldomly preserved. Insight into these features is highly valuable to the understanding the paleoecology, like the feeding and respiratory strategies, of these enigmatic creatures.

One group of diploporan echinoderms which does have an extensive fossil record, is the Holocystites Fauna, primarily known from the …

Interfacing Computational Techniques With Synthetic And Spectroscopic Methods For Research And Education, Nicole Annette Miller

USF Tampa Graduate Theses and Dissertations

Quantum Mechanics (QM) is a powerful tool in computational chemistry with a wide range of applications in both research and education. More and more, the scientific community is tackling research questions of increasing difficulty with an interdisciplinary approach between experimental and computational chemistry. QM methods are now developed to a point where computation can accurately predict chemical properties related to structure and function such as potential energy surfaces (PES), natural bond order analysis (NBO), nuclear magnetic resonance (NMR), and electronic circular dichroism (ECD). Herein, QM methods will be applied to research in peptidomimetics through PES and NBO calculations (Chapter 2 …

The Effects Of Temperature And Oxygen Availability On Aerobic Performance In Three Coastal Shark Species; Squalus Acanthias, Carcharhinus Limbatus, And Carcharhinus Leucas, Alyssa M. Andres

USF Tampa Graduate Theses and Dissertations

Anthropogenically driven climate changes are altering marine habitats globally. Rising sea surface temperatures and coastal eutrophication, arising from global warming and coastal nutrient loading, have resulted in progressive ocean deoxygenation. This may restrict available habitat of marine organisms as studies suggest that the balance between metabolic oxygen demand and environmental supply plays an important role in limiting viable habitat and species fitness. As ectothermic predators, with temperature-dependent metabolism and high metabolic demands, coastal shark species may be susceptible to shifts in ocean temperature and oxygen. Such environmental changes may alter metabolic performance and ultimately success and survival within shark habitat. …

Color Defined: An Autoethnographic Exploration Of How Race, Trauma, Gun Violence, And Grief Connect For A Black Mother, Kokita Dirton Wilson

USF Tampa Graduate Theses and Dissertations

Black mothers are disproportionately affected by fatal shootings and the need for making meaning and finding purpose remains overlooked as a vital component of building resilience in their grief journey. The purpose of this study was to (a) reflect on being a Black mother of a fatal shooting victim; (b) understand the grief and healing process that follows; (c) connect my experiences to those within the larger Black grief community, and (d) by example, help other grieving mothers navigate through their grief struggles. I used two research questions: 1) How can I learn about my grief process by using personal …