Digital Commons Network^™

Repeated Binge Pattern Ethanol Administration During Adolescence Or Adulthood: Long-Term Changes In Voluntary Ethanol Intake And Mesolimbic Dopamine Functionality In Male Rats, Antoniette Michelle Maldonado-Devincci

USF Tampa Graduate Theses and Dissertations

Binge alcohol consumption is a rising concern in the United States, especially among adolescents as during this developmental period alcohol use is usually initiated and has been shown to cause detrimental effects on brain structure and function. These findings have been established through the use of binge models in animals, where animals are repeatedly administered high doses of ethanol typically over a period of three or four days. While such work has examined the effects of a four-day and repeated three-day binge, there has been almost no work conducted aimed at investigating the long-term behavioral and neurochemical and/or functional consequences …

Technology Use As Transformative Pedagogy: Using Video Editing Technology To Learn About Teaching, Michelle Macy

USF Tampa Graduate Theses and Dissertations

Within the paradigm of Sociocultural Theory, and using Activity Theory as a data-gathering and management tool, this microgenetic case study examined the processes - the growth, change, and development – engaged in by student-teachers in a foreign language education program as they worked together to complete an activity. The activity involved digital video recording and editing, mediators which were intended to facilitate the iterative review of and subsequent reflection and action upon the content of the video during its creation.

By investigating the process of contextual interaction between learners and the mediational elements of their environment as the activity progressed, …

A Swmm-5 Model Of A Denitrifying Bioretention System To Estimate Nitrogen Removal From Stormwater Runoff, Michelle D. Masi

USF Tampa Graduate Theses and Dissertations

This research estimates nitrogen removal from stormwater runoff using a denitrifying bioretention system using the USEPA Storm Water Management Model Version 5 (SWMM-5). SWMM-5 has been used to help planners make better decisions since its development in 1971. A conventional bioretention system is a type of Low Impact Development (LID) technology, which designed without a media layer specifically for achieving nitrogen removal. More recently studies have showed that high TN removal efficiencies are possible when incorporating a denitrification media layer. These systems are known as denitrifying bioretention systems, or alternative bioretention systems. LID projects are currently being designed and developed …

Natural Product Drug Discovery Against Tropical Diseases, Wai Sheung Ma

USF Tampa Graduate Theses and Dissertations

This dissertation describes the isolation of secondary metabolites from natural origins through a series of chromatographic techniques and spectrometric characterization in the effort of drug discovery. The isolated compounds obtained were used as drug leads against tropical diseases, namely malaria and leishmaniasis. While first chapter offers an introduction on the use of a natural product by itself as an effective therapeutic and its role on inspiring the discovery of new drugs, the later chapters will concentrate on isolation and characterization of bioactive natural products from an Antarctic sponge and mangrove endophytic fungi during the dissertation work.

The second chapter describes …

Chemical Investigation Of The Antarctic Marine Invertebrates Austrodoris Kerguelenensis & Dendrilla Membranosa And The Antarctic Red Alga Gigartina Skottsbergii, John Alan Maschek

USF Tampa Graduate Theses and Dissertations

The marine realm and, in particular, the Antarctic benthos is largely unexplored and understudied. The chemical investigation reported herein reveals not only the biodiversity, but how that biodiversity manifests remarkable chemical diversity. In our continuing study of the nudibranch Austrodoris kerguelenensis, we have isolated a diverse suite of diterpenoid glyceride esters, palmadorins D - S (2.32 - 2.47), one of which is the first reported halogenated diterpene from a dorid nudibranch. Utilizing genomic data from collaborators, we have investigated the chemical diversity from phylogenetically unique specimens collected in close proximity to one another. Chemical groupings based on comparison of LC/MS …

Investigation Of The Optimal Dissolved Co2 Concentration And Ph Combination For The Growth Of Nitrifying Bacteria, Raymond Anthony Morris

USF Tampa Graduate Theses and Dissertations

Ammonium (NH₄⁺) is a biological nutrient that is transformed in a wastewater treatment plant (WWTP) in a process called activated sludge. This is accomplished in an aerobic environment using microorganisms and inorganic carbon that convert the ammonium to nitrate (NO₃^-). This process is termed nitrification. Removal of ammonium is necessary due to its oxygen demand and toxicity to the environment.

Nitrification is considered a slow process due to the slow growth rate of the nitrifying bacteria. Ammonia oxidizing bacteria (AOB) first covert the ammonium (NH₄⁺) to nitrite (NO₂^-) …

Effectiveness Of In-Line Chlorination Of Gravity Flow Water Supply In Two Rural Communities In Panama, Kevin Orner

USF Tampa Graduate Theses and Dissertations

It is well established that water quality is directly linked to health. In-line chlorination is one technology that can be used in the developing world to potentially inactivate pathogens and improve water quality. The purpose of this study was to determine the effectiveness of the Panamanian Ministry of Health's in-line PVC chlorinator under three different operating conditions in a rural water supply system. Free and total chlorine were measured entering the storage tank, leaving the storage tank, and at three households along the transmission line of the water system in the two rural indigenous communities of Calabazal and Quebrada Mina …

Geometric Transformations In Middle School Mathematics Textbooks, Barbara Zorin

USF Tampa Graduate Theses and Dissertations

This study analyzed treatment of geometric transformations in presently available middle grades (6, 7, 8) student mathematics textbooks. Fourteen textbooks from four widely used textbook series were evaluated: two mainline publisher series, Pearson (Prentice Hall) and Glencoe (Math Connects); one National Science Foundation (NSF) funded curriculum project textbook series, Connected Mathematics 2; and one non-NSF funded curriculum project, the University of Chicago School Mathematics Project (UCSMP).

A framework was developed to distinguish the characteristics in the treatment of geometric transformations and to determine the potential opportunity to learn transformation concepts as measured by textbook physical characteristics, lesson narratives, and analysis …

An Examination Of The Impacts Of Urbanization On Green Space Access And Water Resources: A Developed And Developing World Perspective, Heather E. Wright Wendel

USF Tampa Graduate Theses and Dissertations

This dissertation addresses the impact of urbanization and land use change on the availability and accessibility of two urban amenities that are often inequitably distributed: green space and water features. Diverse methodologies were utilized in order to gain a better understanding of the role of these amenities in improving urban quality of life and integrated water management. Using an interdisciplinary approach, this research provides a unique perspective within both a developed and developing world context by evaluating aspects of urbanization to emphasize more sustainable and integrated approaches to development.

A preliminary analysis highlights potential drivers of green space revitalization in …

The Virtuoso Human: A Virtue Ethics Model Based On Care, Frederick Joseph Bennett

USF Tampa Graduate Theses and Dissertations

The goal of this thesis is to develop the foundation and structure for a virtue ethics theory grounded in a specific notion of care. While there has been a recent revival of interest in virtue ethics theory, the theory has its roots in Aristotle's work as well in the medieval writings of Thomas Aquinas. Aquinas worked out many of Aristotle's ideas in much more detail. However, while Aquinas offers a very rich and compelling ethical theory, it is problematic because it is very tightly wrapped in his theology. A key component in Aquinas's theory is charity. Charity is one of …

Influence Of Temperature On Yolk Resorption By Centropomus Undecimalis Larvae, Claudia Catalina Baron-Aguilar

USF Tampa Graduate Theses and Dissertations

In an effort to determine the optimal temperature for rearing Centropomus undecimalis larvae during the yolk resorption period, larval development was measured under four different temperature regimes (23, 25, 28 and 31 °C). The eggs were incubated at 28 °C until hatching, which occurred at about 17 hours post-fertilization. After hatching, temperatures were adjusted to the respective treatment levels. Measurements were collected from 25 individual larvae across rearing temperatures at the following pre-determined time intervals: at hatching, 24 hours post hatch (hph), 48 hph, and 72 hph. Morphometric measurements were obtained from photomicrographs, including yolk sac length and height, oil …

Development Of An Elisa For Eastern Equine Encephalitis Virus That Can Differentiate Infected From Vaccinated Horses, Andrea Bingham

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus (EEEV) causes a fatal mosquito-borne virus that is vaccine preventable for horses. The conventional serological tests measure antibodies to the structural proteins of EEEV which are also found in the vaccine. This makes it difficult to differentiate infected and vaccinated animals (DIVA). Detection of antibodies to non-structural proteins (NSPs) is a theoretical strategy that would allow you to survey natural infections among vaccinated populations. This test would also allow for more accurate representations of the natural infection rate, vaccination rate, and help identify vaccine failures. The potential uses of the NSPs of Eastern Equine Encephalitis virus …

Stable Isotope Studies Of Paleoenvironment And Paleoclimate From Afar, Ethiopia, Zelalem K. Bedaso

USF Tampa Graduate Theses and Dissertations

The sedimentary deposits of the Hadar Formation at Dikika and the Mount Galili Formation at Galili preserve a wealth of paleoenvironmental and paleoclimatic records spanning the last 5.29 Ma. Stable carbon and oxygen isotopic compositions of herbivore tooth enamel were analyzed for more than 600 specimens of 15 different taxa from 10 stratigraphic intervals. The application of carbon and oxygen isotopes here aims principally at reconstructing shifts in the relative abundance of C₄ grasses, and its implications for climate indicators including temperature, aridity, and seasonality. The full range of δ¹³C_enamel values throughout the Plio-Pleistocene signifies a …

A Comparative Study Of Eucalanoid Copepods Residing In Different Oxygen Environments In The Eastern Tropical North Pacific: An Emphasis On Physiology And Biochemistry, Christine J. Cass

USF Tampa Graduate Theses and Dissertations

The eastern tropical north Pacific (ETNP) is characterized by one of the ocean's most severe midwater oxygen minimum zones (OMZs), where oxygen levels are often less than 5 µM. The copepod family Eucalanidae is a numerically abundant and diverse zooplankton group in the ETNP, and displays a wide range of vertical distributions related to environmental oxygen concentrations. The goal of this dissertation was to develop a better understanding of the ecology, physiology, and biochemistry of closely related copepod species (family Eucalanidae) that inhabit the ETNP OMZ system. This was accomplished through examining different parameters relating to (1) metabolic rates, (2) …

Sexually Explicit, Socially Empowered: Sexual Liberation And Feminist Discourse In 1960s Playboy And Cosmopolitan, Lina Salete Chaves

USF Tampa Graduate Theses and Dissertations

In this thesis, I provide an analysis of 1960s American popular culture by examining Playboy, "The Playboy Philosophy," Cosmopolitan, and Sex and the Single Girl. These cultural artifacts furthered the feminist movement by challenging gender structures and sexuality. I discuss how these publications focused on the advancement of the individual through careerism, consumerism and sexuality. These publications assisted in challenging and breaking down various aspects of gender and sexual boundaries and assisted in reworking social limitations that kept women from advancing themselves outside of the pre-set gender roles of domesticity. Regardless of the traditional feminist critique of …

Inhibition Of P53 Dna Binding Function By The Mdm2 Acidic Domain, Brittany Lynne Cross

USF Tampa Graduate Theses and Dissertations

MDM2 regulates p53 predominantly by promoting p53 ubiquitination. However, ubiquitination-independent mechanisms of MDM2 have also been implicated. Here we show that MDM2 inhibits p53 DNA binding activity in vitro and in vivo. MDM2 binding promotes p53 to adopt a mutant-like conformation, losing reactivity to antibody Pab1620, while exposing the Pab240 epitope. The acidic domain of MDM2 is required to induce p53 conformational change and inhibit p53 DNA binding. ARF binding to the MDM2 acidic domain restores p53 wild type conformation and rescues DNA binding activity. Furthermore, histone methyl transferase SUV39H1 binding to the MDM2 acidic domain also restores p53 wild …

Lead Discovery And Optimization Strategies Towards The Development Of 4(1h)-Quinolones And 1,2,3,4-Tetrahydroacridone Analogs With Antimalarial Activity, Richard Matthew Cross

USF Tampa Graduate Theses and Dissertations

The goal of our research endeavor was to successfully employ modern lead discovery and optimization strategies towards the development and identification of compounds possessing antimalarial activity. Preliminary data from in vitro screening at the Walter Reed Army Institute of Research identified several chemotypes including 4(1H)-quinolones and 1,2,3,4-tetrahydroacridones to have potent antimalarial activities. Multiple synthetic routes were devised and implemented which enabled the rapid preparation and isolation of over 400 structurally diverse 4(1H)-quinolones and 1,2,3,4-tetrahydroacridones.

Our research towards discovering and optimizing antimalarials was inspired from the severe impact malaria has had on our planet especially on impoverished countries. There are over …

Microbial Landscapes Of Corals And Ctenophores, Camille Arian Daniels

USF Tampa Graduate Theses and Dissertations

As technology and engineering allow mankind to survey nature at finer scales, the importance of bacteria has been elucidated in their metabolic diversity, ability to transfer genetic information, involvement in biogeochemical cycling, and sheer abundance. With an individual domain of life unto themselves, this diverse group of microorganisms plays an integral role in facilitating life on land and in the oceans, and is second only to viruses in abundance on Earth. They carve niches in a wide range of environments, including those inhospitable to other life forms, and reside in concert or to the detriment of other microbes and/or hosts …

Population Genetics Of Amphiuma Means And Siren Lacertina In Central Florida, Anna C. Deyle

USF Tampa Graduate Theses and Dissertations

Population structure can be defined as a difference in the frequencies of alleles among populations, and it is often caused by barriers to dispersal. I used mitochondrial DNA sequences to examine the population structure of two large, aquatic salamanders, Amphiuma means (the Two-Toed Amphiuma) and Siren lacertina (the Greater Siren), at two spatial scales in peninsular Florida. Both species appear to have similar life histories, including poor dry land dispersal capabilities, and I hypothesized that they would exhibit significant population structure at both broad and fine scales. Wetland loss and landscape fragmentation are certain to have caused decreases in abundance …

Mechanistic Modeling Of Photocatalytic Water Disinfection, Omatoyo Kofi Dalrymple

USF Tampa Graduate Theses and Dissertations

The main goal of this research was to develop a mechanism-based model for photocatalytic disinfection of bacteria in water using suspended catalyst pthesiss in batch reactors. The photocatalytic disinfection process occurs as a semiconductor photocatalyst, most commonly titanium dioxide (TiO₂), is irradiated with light of wavelength less than 380 nm to produce hydroxyl radicals and other highly reactive oxidants which can inactivate microorganisms. Photocatalytic disinfection involves a complex interaction of many fundamental mechanisms such as light absorption and scattering by semiconductor pthesiss, electrochemical surface reactions, and heterogeneous colloidal stability. Current models, based largely on chemical reacting systems, do …

Guanidines, Amidines And Carbamides As Novel Sigma Receptor Ligands For Post-Stroke Therapeutics, Michelle Yolanda Cortes-Salva

USF Tampa Graduate Theses and Dissertations

Stroke is the 3^rd leading cause of death in the United States. For this reason, it has become our goal to find a drug that is capable of reestablishing intracellular and extracellular Ca⁺² flux upon direct binding to the sigma receptor, which can be delivered easily and efficiently. Our active research focuses the development of guanidine analogues that can serve as therapeutic drugs which target sigma 1 and sigma 2 receptors having a direct effect on Ca⁺² levels on the cell. The use of symmetrical guanidine analogues such as N,N'-di-o-tolyl guanidine (o-DTG) as therapeutic drugs for ischemic …

Anaerobic Membrane Bioreactor (Anmbr) For Treatment Of Landfill Leachate And Removal Of Micropollutants, Anh Tien Do

USF Tampa Graduate Theses and Dissertations

To date, most studies on the fate and removal of endocrine disrupting compounds (EDCs) and pharmaceutical and personal care products (PPCPs) in wastewater focus on their fate in municipal wastewater treatment plants, and mostly under aerobic condition. There are limited studies related to anaerobic condition and (to our knowledge) no study on the removal of EDCs in landfill leachate by AnMBR. Moreover, for most studies under anaerobic condition, the removal of EDCs was only reported in the liquid phase; solid phase extraction was not reported, thereby preventing mass balance in the studies.

This research was conducted to investigate the potential …

Philosophical Precursors To The Radical Enlightenment: Vignettes On The Struggle Between Philosophy And Theology From The Greeks To Leibniz With Special Emphasis On Spinoza, Anthony John Desantis

USF Tampa Graduate Theses and Dissertations

My dissertation lays out some of the chief philosophical precursors to Jonathan Israel's Radical Enlightenment. It investigates the principal question of Will Durant's The Age of Voltaire: "How did it come about that a major part of the educated classes in Europe and America has lost faith in the theology that for fifteen centuries gave supernatural sanctions and supports to the precarious and uncongenial moral code upon which Western civilization has been based?" The aim of this project is both broad and specific: the first is to provide a general history of the philosophical precursors to the Radical Enlightenment up …

Development Of A Non-Invasive Electrode For Intradermal Electrically Mediated Dna Vaccination, Amy Lynn Donate

USF Tampa Graduate Theses and Dissertations

Current progress in the development of vaccines has decreased the incidence of fatal and non-fatal infections and increased longevity. However, new technologies need to be developed to combat an emerging generation of infectious diseases. DNA vaccination has been demonstrated to have great potential for use against a wide variety of diseases. Alone, this vaccine technology does not generate a significant immune response for vaccination, but combined with delivery by electroporation (EP), can enhance plasmid expression and immunity against the expressed antigen. Most EP systems, while effective, can be invasive and painful making them less desirable for use in vaccination. Our …

Population Biology, Ecology, And Ecosystem Contributions Of The Eastern Oyster (Crassostrea Virginica) From Natural And Artificial Habitats In Tampa Bay, Florida, Michael Drexler

USF Tampa Graduate Theses and Dissertations

The objective of this project was to document the status of oysters, Crassostrea virginica, from non-reef habitats throughout Tampa Bay, Florida, and assess the ecosystem contributions of those populations relative to reef-dwelling oysters. The aspects of oyster ecology studied here include condition, prevalence and intensity of disease (Perkinsus marinus - dermo), reproductive activity (including stage, fecundity, and juvenile recruitment), adult oyster density, and the faunal community associated with the oysters.

The predominant source of variation was seasonal, with lesser contributions among sites, and in most cases, little or no effect of the habitat type. Oysters populations from each habitat …

Does D-Cycloserine Augmentation Of Cbt Improve Therapeutic Homework Compliance For Pediatric Obsessive Compulsive Disorder?, Jennifer M. Park

USF Tampa Graduate Theses and Dissertations

D-cycloserine (DCS), a partial agonist that acts on the N-methyl-D-aspartate (NMDA) receptor of the glutamatergic receptor complex, may enhance fear extinction learning during exposure-based therapy. Clinical studies in adults with obsessive-compulsive disorder (OCD) and non-OCD anxiety disorders - and a recent trial in pediatric OCD - have shown that DCS can improve treatment response to exposure therapy relative to placebo and exposure therapy. Some have hypothesized that improved treatment response is a function of increased compliance and engagement in therapeutic homework tasks, a core component of behavioral treatment. The present study examined the relationship between DCS and homework compliance in …

"We Have Never Known What Death Was Before"--A Just War Doctrine Critique Of U.S. History Textbooks, Mark Pearcy

USF Tampa Graduate Theses and Dissertations

Textbooks are a significant element of the social studies curriculum and teacher pedagogical choice (Apple, 2004; Apple & Christian-Smith, 1991). Students' views of American history are dramatically affected by the textbook narratives to which they are exposed, and teachers often tilt their curricular choices based on the textbooks available to them (Luke, 2006; Schug, Western & Enochs, 1997). The history of our nation's armed conflicts is often presented, through our textbooks and our pedagogy, as a history of reluctant violence, which promotes a particular moral agenda that exerts control over our students' future beliefs and decisions. This is particularly important …

Delinquencies, Bryan Thomas Rice

USF Tampa Graduate Theses and Dissertations

Delinquencies brings together forty-eight poems that reflect some of the aesthetic, philosophical and cultural interests I've attended over the last five years or so--namely, ideas related to failure and abhorrent behavior.

Guiding Electric Fields For Electroporation Applications, Jose Rey

USF Tampa Graduate Theses and Dissertations

Electroporation is the critical step in an electric field mediated drug or gene delivery protocol. Electroporation based protocols have been successfully demonstrated in cancer clinical trials, however, its impact in other applications is still under investigation. A significant roadblock to long term functioning of implantable biosensors in vivo is the tissue reaction in the form of fibrous encapsulation that results in reduced transport to the sensing element of the biosensor. In vivo gene electroporation has a great potential as a means to modify the transport properties of tissues in the proximity of the sensing element of implantable biosensors.

This dissertation …

Synthesis And Antimicrobial Activities Of S,S'-Heterosubstituted Disulfides, Praveen Ramaraju

USF Tampa Graduate Theses and Dissertations

Antibiotic resistance is a particularly critical health concern and has increased dramatically over the past two decades. For over a decade the Turos laboratory has been designing small molecules to target pathogenic microbes such as Staphylococcus aureus and the resistant variants like methicillin-resistant Staphylococcus aureus (MRSA). Previously, N-thiolated Β-lactams, N-thiolated 2-oxazolidinones and aromatic disulfides that were synthesized in Dr. Turos' lab have shown strong activity against these bacteria. The present work describes the synthesis and antimicrobial activities of a related structural class called S,S'-heterosubstituted disulfides. For ages, sulfur (elemental) has been used as an antibacterial for controlling infestation and bacterial …