Digital Commons Network^™

Aberrant Sialylation Alters Cardiac Electrical Signaling, Andrew Ednie

USF Tampa Graduate Theses and Dissertations

In the heart, electrical signaling is responsible for its rhythmicity and is necessary to initiate muscle contraction. The net electrical activity in a cardiac myocyte during a contraction cycle is observed as the action potential (AP), which describes a change in membrane potential as a function of time. In ventricular cardiac myocytes, voltage-gated sodium channels (Nav) and voltage-gated potassium channels (Kv) play antagonistic roles in shaping the AP with the former initiating membrane depolarization and the latter repolarizing it. Functional changes in the primary cardiac Nav isoform, Nav 1.5, or any one of the many Kv isoforms expressed in the …

Paleo-Indian To Spanish Occupation Around Choctawhatchee Bay, Northwest Florida, As Documented In A Private Artifact Collection, Deena Woodward

USF Tampa Graduate Theses and Dissertations

This project documents and analyzes a substantial private collection of artifacts from archaeological sites around Choctawhatchee Bay, northwest Florida. The goals are to determine what the materials can contribute to the archaeological record, how they enhance our knowledge of the people who lived there over the past 2000 years, and how this information can be used to expand or contradict models of prehistoric lifeways in this region. This is done by comparing artifact assemblages, looking at site distribution patterns, and using pXRF trace-element analysis to compare Late Archaic clay objects with those from elsewhere along the Gulf.

Examination of the …

Assessment Of Diver Impact During The Spiny Lobster Sport Season, Florida Keys, Usa, Mark Lewis Hartman

USF Tampa Graduate Theses and Dissertations

The Spiny Lobster (Panulirus argus) fishery in Florida is closed during the spawning season (March-July) except for a two-day recreational `miniseason' for sport divers in July, several days prior to the opening of the commercial fishing season. In Monroe County, recreational fishers, who possess a valid Saltwater Fishing License with crawfish stamp, are allowed to harvest six lobsters per day, each with a minimum carapace length of 76.2 mm (3.0 inches). During these two days, approximately 50,000 people attempt to catch lobster, and the number of boats visiting the reef has been estimated to be up to 900 times higher …

Lower Sacraments: Theological Eating In The Fiction Of C. S. Lewis, Gregory Philip Hartley

USF Tampa Graduate Theses and Dissertations

For years, critics and fans of C. S. Lewis have noted his curious attentiveness to descriptions of food and scenes of eating. Some attempts have been made to interpret Lewis's use of food, but never in a manner comprehensively unifying Lewis's culinary expressions with his own thought and beliefs. My study seeks to fill this void. The introduction demonstrates how Lewis's culinary language aggregates through elements of his life, his literary background, and his Judeo-Christian worldview. Using the grammar of his own culinary language, I examine Lewis's fiction for patterns found within his meals and analyze these patterns for theological …

Stable Isotope Analysis Of Busycon Sinistrum To Determine Fort Walton-Period Seasonality At St. Joseph Bay, Northwest Florida, Ryan Michael Harke

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Recent archaeological investigations indicate that coastal Fort Walton cultures in the St. Joseph Bay region of northwest Florida emphasized marine and estuarine foraging. These late prehistoric (A.D. 1000-1500) peoples collected fish, shellfish, and other aquatic resources. At the Richardson's Hammock site (8Gu10), radiocarbon-dated to about A.D. 1300, as at dozens of other shell middens around this salty bay, large gastropods were a major subsistence component. This adaptation is in sharp contrast with that of contemporaneous inland Fort Walton societies, who relied on maize agriculture. It is unknown whether coastal groups represent separate hunter-gatherer-fisher populations or seasonal migrations by inland …

No Honor Among Snails: Conspecific Competition Leads To Incomplete Drill Holes In The Naticid Gastropod Neverita Delessertiana (R Cluz), Jack A. Hutchings

USF Tampa Graduate Theses and Dissertations

The fossil record of drilling predation has been widely used to study predator-prey interactions and their relative importance on long-term evolutionary processes. Incomplete drill holes have been interpreted as indicators of failed attacks due to well-defended prey. However, this interpretation is based on pair-wise interactions between one predator and one prey, a condition commonly compromised in nature. The hypothesis that interference among drilling predators leads to an increase in the relative frequency of incomplete drill holes was tested in the laboratory using the naticid Neverita delessertiana (R cluz) and a common prey, the bivalve Chione elevata (Say). The experiment consisted …

Community As Metaphor: Dialectical Tensions Of A Racially Diverse Organization, Joseph Jacob Jenkins

USF Tampa Graduate Theses and Dissertations

In recent years, a sense of community has declined throughout the United States. Common Point Community Church has responded to this trend by prioritizing "community" as an organizational metaphor. The present study explores how this metaphor is co-constructed through the communication practices of current organizational leaders and members. I begin this process, first, by positioning the study within existing literature on institutional theory, institutional legitimacy, community, community of practice, social construction of race, sensemaking, organizational metaphor, tension-centered approach, and dialectic theory. Building upon more than three years of ethnographic field work, I then outline the study's context and methodology. Next, …

Effects Of Gps Error On Animal Home Range Estimates, Garrett Hyzer

USF Tampa Graduate Theses and Dissertations

This study examined how variables related to habitat cover types can affect the positional accuracy of Global Positioning System (GPS) data and, subsequently, how wildlife home range analysis can be influenced when utilizing this inaccurate data. This study focused on measuring GPS accuracy relative to five habitat variables: open canopy, sparse canopy, dense canopy, open water, and building proximity. The study took place in Hillsborough County, in residential areas that contain all of these habitat types. Five GPS devices, designed for wildlife tracking purposes, were used to collect the data needed for this study. GPS data was collected under the …

Development And Optimization Of Kinetic Target-Guided Synthesis Approaches Targeting Protein-Protein Interactions Of The Bcl-2 Family, Sameer Shamrao Kulkarni

USF Tampa Graduate Theses and Dissertations

Kinetic target-guided synthesis (TGS) and in situ click chemistry are among unconventional discovery strategies having the potential to streamline the development of protein-protein interaction modulators (PPIMs). In kinetic TGS and in situ click chemistry, the target is directly involved in the assembly of its own potent, bidentate ligand from a pool of reactive fragments. Herein, we report the use and validation of kinetic TGS based on the sulfo-click reaction between thio acids and sulfonyl azides as a screening and synthesis platform for the identification of high-quality PPIMs. Starting from a randomly designed library consisting of nine thio acids and nine …

Archaeological Survey And Testing On St. Vincent Island, Northwest Florida, Elicia Victoria Kimble

USF Tampa Graduate Theses and Dissertations

St. Vincent Island is one of the barrier islands in the Florida panhandle between Apalachicola Bay and the Gulf of Mexico. The St. Vincent National Wildlife Refuge encompasses all 5000 hectares of the island. Archaeological fieldwork in the summer of 2009 included a survey of the entire island and a test unit at one of the island's richest sites. In spring of 2010 a second test unit was excavated at another archaeologically rich site. A total of 16 known sites were investigated and two newly discovered sites recorded. This research combines all these data with information obtained from existing artifact …

Precipitation Of Phosphate Minerals From Effluent Of Anaerobically Digested Swine Manure, Alex Y. Lin

USF Tampa Graduate Theses and Dissertations

Swine production represents approximately 40% of the world's meat production, and its wastes contain high concentrations of organic carbon, nitrogen (N), and phosphorus (P). Anaerobic digestion is an increasingly popular technology for treating animal wastes while simultaneously generating energy. Its propagation and ability to solubilize organic N and P make adding a struvite recovery process attractive. Recovering struvite (MgNH4PO4) from anaerobically digested swine waste can address global P shortages, meet P discharge guidelines, and produce slow-release fertilizer, which can be sold for revenue.

Anaerobic digesters were operated with at organic loading rates of 3.4-3.9 g volatile solids per liter per …

Semantic Feature Distinctiveness And Frequency, Katherine Marie Lamb

USF Tampa Graduate Theses and Dissertations

Lexical access is the process in which basic components of meaning in language, the lexical entries (words) are activated. This activation is based on the organization and representational structure of the lexical entries. Semantic features of words, which are the prominent semantic characteristics of a word concept, provide important information because they mediate semantic access to words. An experiment was conducted to examine the importance of semantic feature distinctiveness and feature frequency in accessing the lexical representations of young and older adults in an off-line task using features of animals. The McRae, Cree, Seidenberg, and McNorgan (2005) feature norm corpus …

"Planting Wholesome Seeds": Organic Farming And Community Supported Agriculture At Sweetwater Organic Community Farm, Philip R. Mcnab

USF Tampa Graduate Theses and Dissertations

Sweetwater Organic Community Farm is an organic farm and environmental education center located in Tampa, Florida. The farm employs the community supported agriculture (CSA) model, in which members pay a single fee before the growing season begins and receive a weekly or biweekly share of the ongoing harvest in return. Using multiple ethnographic methods, this research aimed to understand the daily operations at Sweetwater as well as the perceptions of staff and CSA members. Findings indicated that there were myriad perceived advantages of organic agriculture but also imposing challenges that needed to be overcome. Moreover, staff members acknowledged the challenges …

A Longitudinal Examination Of High School Students' Group Membership In A Dual-Factor Model Of Mental Health: Stability Of Mental Health Status And Predictors Of Change, Melanie M. Mcmahan

USF Tampa Graduate Theses and Dissertations

A dual-factor model of mental health includes indicators of wellness (i.e., subjective well-being; SWB) and psychopathology (i.e., internalizing and externalizing behavior problems) in defining youth mental health. In this model, four categories of psychological functioning with distinct levels of SWB and psychopathology emerge, including two that are overlooked (i.e., Vulnerable and Symptomatic but Content) in traditional assessments that assume SWB and psychopathology are opposite ends of the same continuum. The present study investigated the 1-year stability of adolescent mental health as classified by a dual-factor model, and identified predictors of stability and change, in a sample of 425 high school …

Circling Back Home: A Lifelong Odyssey Into Feminism, Scott Leslie Neumeister

USF Tampa Graduate Theses and Dissertations

What happens when a classroom becomes more than just a site of intellectual growth and evolves into a locus of emotional, social, and spiritual transformation? What happens when a student in such a classroom also occupies the role of teacher and desires to reproduce such a transformative environment for his students? In brief, this thesis answers these questions by offering a narrative and critique of my personal "conscientization" via feminism and elucidates the theory behind, my approaches toward, and the results of my bringing graduate-level feminist theory and pedagogy to a middle school English classroom. I examine how my experiences …

Development And Application Of Its Operations Evaluation Models (Itsoems), Gummadavelly Narasimha Murthy

USF Tampa Graduate Theses and Dissertations

Intelligent Transportation Systems (ITS) play an important role in supporting motorists and first responders to handle major incidents, hazmat spills, security measures and other emergency operations. Increasingly, technology advancements and applications are providing invaluable integration opportunities to interconnect safety, maintenance, ITS, traffic operations, facilities, and transportation equipment programs to maximize available resources and achieve efficiency in transportation operations.

This research focuses on one of the prominent ITS devices: Dynamic Message Signs (DMS) operations. Using real time incident management operations data and ITS operations data, an attempt is made to develop ITS Operations Evaluations Models (ITSOEMs) that are capable of quantifying …

Socioscientific Issues: A Path Towards Advanced Scientificliteracy And Improved Conceptual Understanding Of Socially Controversial Scientific Theories, Dean William Pinzino

USF Tampa Graduate Theses and Dissertations

Abstract

This thesis investigates the use of socioscientific issues (SSI) in the high school science classroom as an introduction to argumentation and socioscientific reasoning, with the goal of improving students' scientific literacy (SL). Current research is reviewed that supports the likelihood of students developing a greater conceptual understanding of scientific theories as well as a deeper understanding of the nature of science (NOS), through participation in informal and formal forms of argumentation in the context of SSI. Significant gains in such understanding may improve a student's ability to recognize the rigor, legitimacy, and veracity of scientific claims and better discern …

Responses To Chemical Exposure By Foraminifera: Distinguishing Dormancy From Mortality, Benjamin James Ross

USF Tampa Graduate Theses and Dissertations

The Deepwater Horizon blowout in 2010 released an estimated 4.9 million barrels of crude oil into the Gulf of Mexico in the 83 days between the initial explosion and the capping of the well. Response included extensive use of Corexit© oil dispersant. Although South Florida was spared exposure by currents, this event highlights the need for effective bioassay organisms for coral reefs. Amphistegina spp. are benthic foraminifers that host diatom symbionts in a relationship similar to that of coral and their zooxanthellae. Amphistegina spp. occur abundantly in reef communities nearly worldwide, are easily collected and maintained in culture, and are …

Mas And The Indigenous People Of Bolivia, Maral Shoaei

USF Tampa Graduate Theses and Dissertations

In the past several decades, social movements have spread all across Latin America, sparking hope for change. This thesis analyzes the well-organized mobilizations of the indigenous people of Bolivia and how they have been able to incorporate themselves in state apparatuses, including the election of its first indigenous president, Evo Morales of the Movement towards Socialism (MAS) party. The case studied her provides insight into the processes if how political representation was achieved by Bolivia's indigenous people who were for centuries excluded from the political, social and economic arena. It also analyzes the outcomes of Morales' policy changes from 2006 …

Dolphin Sound Production And Distribution On The West Florida Shelf, Peter Simard

USF Tampa Graduate Theses and Dissertations

This dissertation is an investigation of dolphin sound production and distribution off west central Florida. Although a wealth of information exists on the production of common sounds (whistles, echolocation) made by captive, trained dolphins, far less is known about free-ranging dolphin sound production and of unusual sounds. In addition, while inshore dolphin populations or communities are the subjects of research projects in many locations, dolphins in offshore waters are less commonly studied. The objectives of this dissertation were to contribute information on free-ranging dolphin sounds and continental shelf dolphin distribution.

While echolocation has been rigorously studied in captive, trained dolphins, …

Environmental Change And Place-Based Identities: Sponge Fishing In Tarpon Springs, Florida, Michael Suver

USF Tampa Graduate Theses and Dissertations

Abstract

As the environmental consequences of urbanization and climate change become apparent in coastal communities, it has become important to understand how residents of these communities experience and approach their changing environments. This becomes especially significant in places where nature-based livelihoods constitute a major part of the economy. This thesis focuses on the city of Tarpon Springs, located along the Gulf of Mexico in Florida, where sea sponge fishing is an important contributor to the local economy while also being central to place-based tourist and ethnic identities. It seeks to understand how environmental changes in the sea sponge economy will …

Design Of Novel Inhibitors For Infectious Diseases Using Structure-Based Drug Design: Virtual Screening, Homology Modeling And Molecular Dynamics, Divya Ramamoorthy

USF Tampa Graduate Theses and Dissertations

The main aim of the study in this thesis was to use structure-based protocols to design new drugs for enzymes, DXS and DXR in the non mevalonate pathway. Another aim of this study was to identify the dimer interface in E.coli FabH as an allosteric binding site for designing new class of anti-infective drugs. We have attempted to identify potential inhibitors for DXS by docking the NCI Diversity set compounds, compound libraries available from GSK-MMV and St. Jude's Children's research center. FabH dimer interface has been identified as a potential target using SiteMap, Alanine mutagenesis and docking studies.

The first …

Assessing The Reuse Potential Of Wastewater For Irrigation: The Removal Of Helminth Eggs From A Uasb Reactor And Stabilization Ponds In Bolivia, Matthew Eric Verbyla

USF Tampa Graduate Theses and Dissertations

Extreme hunger, malnutrition, and the lack of access to sanitation are among the most pressing development challenges, but the world is not on track to meet the targets that have been established by the Millennium Development Goals. The integration of wastewater treatment and food production systems allows for the recovery of resources from wastewater, and can provide an important solution to meet the sanitation needs of growing urban populations and provide periurban farmers with a consistent supply of water and nutrients. Stabilization ponds have been long considered to be an appropriate technology for wastewater reuse systems in developing countries, but …

Ontogeny And Littoral Structure Of Lakes Created On Phosphate Mined Lands Of Central Florida, Chrysoula Mitraki

USF Tampa Graduate Theses and Dissertations

Created lakes are an end product of phosphate mining in Florida. Beginning in 1975, Florida reclamation rules set criteria for phosphate created lakes aiming to approximate the structure of natural lakes, and emphasizing extensive littoral zones with both emergent and submersed vegetation.

Lake development relative to lake age and design were examined for 22 phosphate lakes representing a 40-year age trajectory, utilizing water quality and benthic invertebrate communities from littoral and deep-water locations (Chapter 1). The relative importance of morphometry and lake age in the development of littoral plant communities was examined using multiple vegetation surveys throughout the year, in …

Aging Of Florida Blue Crabs, Callinectes Sapidus, Through The Biochemical Extraction Of Lipofuscin, Claire Elizabeth Crowley

USF Tampa Graduate Theses and Dissertations

The blue crab, Callinectes sapidus, represents an ecologically and economically important component of marine and estuarine ecosystems. In Florida, blue crab landings accounted for $9.6 million dollars during the 2010 fishing season. Accurate stock assessments for this valuable fishery are essential. Age is a critical biological component of accurate stock assessments; however, blue crabs and other crustaceans are especially difficult to age because of the complex nature of discrete growth. Biochemical extraction of an aging pigment, lipofuscin, was developed using blue crab eyestalks. The current study investigated the effects of freezing preservation on lipofuscin extracts and examined whether the …

A Novel Device And Nanoparticle-Based Approach For Improving Diagnosis And Treatment Of Pelvic Inflammatory Disease, Natasha Faith Cover

USF Tampa Graduate Theses and Dissertations

Pelvic Inflammatory Disease (PID) is one of the most common causes of morbidity in women. PID is a polymicrobial infection of the female reproductive tract, and is associated with pelvic pain, abnormal uterine bleeding, and tubal damage that can lead to ectopic pregnancies and infertility. It is curable but the effects of PID can be permanent if not properly diagnosed and treated. PID presents as a spectrum of disease and is often missed at early stages; even acute PID can be difficult to diagnose, as there is no single conclusive diagnostic test. Currently, PID is identified and treated syndromically because …

Multisensory Integration In Shark Feeding Behavior, Jayne M. Gardiner

USF Tampa Graduate Theses and Dissertations

Multimodal sensory input directs simple and complex behaviors in animals. Most research to date has been limited to studies of individual senses rather than multiple senses working together, leading to important advances in our comprehension of the sensory systems in isolation, but not their complementary and alternative roles in difficult behavioral tasks, such as feeding. In the marine environment, a prey item might emit an odor, create a hydrodynamic disturbance, such as from gill movements or swimming, be visible to the predator, produce a sound, and/or produce a weak electrical field. Therefore, the goal of this study was to investigate …

The Invasive Cactus Moth, Cactoblastis Cactorum: Host Plant Testing, Species Interactions, And Effects On Local Opuntia Populations, Heather Jezorek

USF Tampa Graduate Theses and Dissertations

The invasive cactus moth, Cactoblastis cactorum, poses a threat to opunitoid cacti species of North America. The following work contains four separate studies investigating C. cactorum host plant preference and performance, predation and parastitism of C. cactorum, effects of C. cactorum on local Opuntia populations, and associational effects of host and non-host plants on C. cactorum and native Opuntia-feeding herbivores. We found that, among southwestern and Mexican opuntioid taxa, moths preferred O. engelmannii var. linguiformis and var. engelmannii for oviposition, while Consolea rubescens and O. streptacantha were superior larval hosts. Oviposition was best predicted by number of cladodes and degree …

An Examination Of Diet, Acculturation And Risk Factors For Heart Disease Among Jamaican Immigrants, Carol Renee Oladele

USF Tampa Graduate Theses and Dissertations

Background: The South Florida region is home to over 85,000 Jamaican immigrants. Yet, little is known about the dietary intakes and predictors of risk of disease within this immigrant group. An assessment of dietary intakes and the development of dietary intake methodologies specific to the Jamaican population was important as it permitted accurate estimation of the nutrient intakes of this immigrant population whose dietary habits are not well documented. In addition, nothing is known about the prevalence of risk factors for heart disease or factors influencing risk factors among this immigrant group. The purpose of this study was to assess …

Identification Of Novel Stat3 Target Genes Associated With Oncogenesis, Rachel Haviland

USF Tampa Graduate Theses and Dissertations

Cytokine and growth factor signaling pathways involving STAT3 are frequently constitutively activated in many human primary tumors, and are known for the transcriptional role they play in controlling cell growth and cell cycle progression. However, the extent of STAT3's reach on transcriptional control of the genome as a whole remains an important question. We predicted that this persistent STAT3 signaling affects a wide variety of cellular functions, many of which still remain to be characterized.

We took a broad approach to identify novel STAT3 regulated genes by examining changes in the genome-wide gene expression profile by microarray, using cells expressing …