Digital Commons Network^™

Development Of A Tool For Pressure Ulcer Risk Assessment And Preventive Interventions In Ancillary Services Patients, Monica Shutts Messer

USF Tampa Graduate Theses and Dissertations

The incidence of nosocomial pressure ulcers has continued to increase in U.S. hospitals over the past 15 years despite the implementation of national preventive guidelines and the wide-spread use of validated risk assessment tools. The majority of preventive efforts and tools have been focused primarily on patients who are bed-ridden or immobile for extended periods. What has not been well studied or identified is the potential risk for pressure injury to patients undergoing diagnostic procedures in hospital ancillary units where extrinsic risk factors such as high interface pressures on procedure tables and friction and shear from positioning and transport can …

Testing A Model Of Bacterial Vaginosis Among Black Women, Jessica Brumley

USF Tampa Graduate Theses and Dissertations

Bacterial Vaginosis is an inbalance of vaginal flora which has been associated with increased risk of numerous gynecological and obstetric morbidities including increased risk of acquisition of HIV from an infected partner and increased risk of preterm delivery. Black race has been consistently identified as a risk factor for BV. Black women also suffer from significant disparities in most of the morbidities also associated with BV when compared to women of other ethnicities and races. Traditional predictors of BV such as douching practices and sexual behaviors do not fully account for the racial disparities in BV prevalence. Researchers have begun …

Investigation And Synthesis Of Novel Graphene-Based Nanocomposites For Hydrogen Storage, Anthony Joseph D'Angelo

USF Tampa Graduate Theses and Dissertations

It is of great interest to develop and utilize a high surface area material with optimized hydrogen sorption properties. The need for a renewable energy source to replace automobile gasoline has become more critical in the past decade. Hydrogen is a viable fuel source for automobile usage; however, the question of how hydrogen will be safely and efficiently stored still remains. Critical factors for optimum hydrogen storage include ambient conditions and low activation temperature for adsorption and desorption phenomena. In order for optimum hydrogen adsorption to be achieved, the properties of (1) high surface area, (2) optimum hydrogen adsorption energy, …

Rainwater Harvesting Storage Methods And Self Supply In Uganda, Jonathan Peter Blanchard

USF Tampa Graduate Theses and Dissertations

Self supply is an emerging approach to water supply which focuses on fostering household investment in incremental improvements to their water sources. When successful, it can lower costs and increase sustainability by offering users a larger share

of ownership in their own supply, and harnessing the already existing strengths of a community rather than trying to impose an external perspective. In addition to well upgrading and source protection, one of the key self supply areas is rainwater harvesting.

Uganda has a diverse selection of rainwater storage options, but many of them are scattered and disparate.

The objective of this study …

Examining The Economic Costs And Sources Of Potable And Nonpotable Water In Northern Mexico, Kathryn Marie Marlor

USF Tampa Graduate Theses and Dissertations

Water availability and the cost of different water sources have been studied at great length. However, information is still needed to determine the policy directions to be undertaken by nations that have not yet achieved universal coverage of an improved water source. To further examine differences in water availability and pricing in the context of the developing world, three communities in Northern Mexico were surveyed to determine the differences in water distribution schemes and associated costs between rural and urban centers. It was observed that rural communities without a piped water supply paid 13 percent more for potable water supplies …

Evidence For Viral Infection In The Copepods Labidocera Aestiva And Acartia Tonsa In Tampa Bay, Florida, Darren Stephenson Dunlap

USF Tampa Graduate Theses and Dissertations

Mesozooplankton are of critical importance to marine food webs by transferring energy from the microbial food web to higher trophic levels and depositing energy to the deeper ocean layers through fecal deposition. While decades of research have shown that viruses have significant impacts in the oceans, and infect a wide range of organisms from bacteria to whales, there is still little known about the impacts of viruses on the mesozooplankton community. As copepods are the most abundant mesozooplankton group, this study sought to characterize the viruses present in natural populations of the calanoid copepods Acartia tonsa and Labidocera aestiva in …

Out Of Our Depth: Hyper-Extensionality And The Return Of Three-Dimensional Media, Justin Alan Brecese

USF Tampa Graduate Theses and Dissertations

This work theorizes the contemporary attraction to three-dimensional media. In doing so, it reframes ongoing debates surrounding digital three-dimensional media in order to critique the neoliberal social relations such media engender. I argue that the contemporary interest in dimensionality, especially regarding digital media, is symptomatic of a broad cultural shift, wherein millions of lives are now essentially being lived through two-dimensional, "flat" media, which have consequently generated a lack of spatial relationships and a craving or desire for "depth." This "desire for depth" has arisen in contemporary society because people are being "spread too thin" through a combination of the …

Postcolonial Religion And Motherhood In The Novels By Louise Erdrich And Alice Walker, Kateryna Chornokur

USF Tampa Graduate Theses and Dissertations

This thesis is a comparative analysis of the works of the Native American author Louise Erdrich (Love Medicine, Tracks) and the African American writer Alice Walker (The Color Purple). Originating from different cultural traditions, Native American and African American women writers address common themes in their novels because of their common colonial background. One of the main themes in their writings is that of religion. Despite becoming victims of Christianity used as a means of cultural colonization, both African American and Native American communities reinterpret it in terms of their traditional religious beliefs and create a new, unique hybridized form …

Overcoming Limitations Of Serial Audio Search, Isabela Cordeiro Ribeiro Moura Hidalgo

USF Tampa Graduate Theses and Dissertations

The typical approach for finding audio recordings, such as music and sound effects, in a database is to enter some textual information into a search field. The results appear summarized in a list of textual descriptions of the audio files along with a function for playing back the recordings. Exploring such a list sequentially is a time-consuming and tedious way to search for sounds. This research evaluates whether searching for audio information can become more effective with a user interface capable of presenting multiple audio streams simultaneously.

A prototype audio player was developed with a user interface suitable for both …

Technical And Economic Assessment Of Adobe As The Primary Building Material On The Water Yield Of A Single Basin Solar Still, Nathan Daniel Manser

USF Tampa Graduate Theses and Dissertations

It is estimated that only one percent of global freshwater is available to humans, with nearly three billion people living in water scarce conditions. Populations living in impoverished settings are particularly vulnerable to water related illnesses, with approximately 2.2 million people dying each year from to waterborne illnesses. This research uses modeling and field studies to assess the quantity, quality, and economics of distillate produced for drinking water from a brackish water source using two single-sloped, single-basin distillation reactors. The reactors were constructed from adobe and concrete in an arid rural community in San Luis Potosí, Mexico and tested from …

Behavioral And Histological Effects Of Traumatic Brain Injury On Alzheimer's Disease Transgenic Mice, Sara Leilani Kellogg

USF Tampa Graduate Theses and Dissertations

The main objective of this study was to elucidate the possible mechanistic link between traumatic brain injury (TBI) and Alzheimer's disease (AD) using an animal model. We examined behavioral and histological effects of TBI in pre-symptomatic AD-transgenic mice (C57B6/SJL/SwissWebster/B6D2F1). In previous studies, these mice displayed AD-like behavioral deficits by 15-17 months of age and AD-like neuropathology as early as six months of age. To clarify the effects of TBI on these mice, the present study began when they were about three months of age and the study ended when they were about five months of age. As a control, non-transgenic …

Removal Of Bisphenol A Model Compounds And Related Substances Using Octolig®, Rachael Josephine Alessio

USF Tampa Graduate Theses and Dissertations

Bisphenol A used in the production of polycarbonate plastics and epoxy resins is ubiquitous in the environment. The raw material is released to the environment during the manufacturing process and by leaching from consumer products. Recent studies are suggesting that low-dose amounts of Bisphenol A may have adverse health effects on humans. The possibility of removing Bisphenol A from natural water sources or from solvents used to extract the material from consumer products before they enter the market has been studied. The use of model compounds and related substances (4-isopropylphenol, 4-(t-butyl) phenol, and nitrophenols) have been used to study their …

Effect Of Platinum Particle Size On The Sulfur Deactivation Of Hydrogenation, Lyndsey Michelle Baldyga

USF Tampa Graduate Theses and Dissertations

A large concern of the fossil fuel and renewable energy industries is the sulfur poisoning of catalysts. In the case of noble metals, such as platinum, it is seen that there is a size trend associated with the level of activity in the presence of sulfur. Smaller nanoparticles could be more tolerant due to sulfur surface vacancies. On the other hand, larger particles could have less deactivation because the sulfur is more attracted to the smaller particles and the sulfur molecules bind stronger to these smaller particles.

The size effect of sulfur deactivation was investigated by testing four sizes of …

Compound-Specific Stable Isotopic Analysis Of Protein Amino Acids: Ecological Applications In Modern And Ancient Systems, Greg Ellis

USF Tampa Graduate Theses and Dissertations

Stable isotopic analysis of the major biochemically important elements is an increasingly utilized tool in the study of ecology. Patterns of isotopic fractionation in carbon and nitrogen are used to determine trophic linkages, nutrient pathways, and sources of primary production in numerous contexts. Traditional techniques rely on measurements made of bulk samples such as tissue, but emerging methods using individual chemical compounds provide a means of achieving deeper understanding in a variety of inquiries.

Amino acids, as the building blocks of proteins, are the dominant nitrogen-bearing biomolecules and are a major constituent of all life. Patterns of isotopic fractionation during …

Evaluation Of Urinary Pesticide Biomarkers Among A Sample Of The Population In The United States, Alex Lance Lebeau

USF Tampa Graduate Theses and Dissertations

Pesticide use in the United States continues to attract negative public attention. In recent years, this attention has focused on the effects that chronic, low-level pesticides may have, especially on children and various sub-populations. Over the past decade, studies have attempted to correlate negative health effects with detections of pesticide biomarkers in biological media. The current research investigates biomarker of exposure levels in a sample of the United States population. Data from the 2001-2002 NHANES dataset (n=11,039) was evaluated. The detection frequency of urinary biomarkers of exposure and the geometric mean from the NHANES pesticide dataset (n=3,152) were determined. Of …

The Creation Of A Robotics Based Human Upper Body Model For Predictive Simulation Of Prostheses Performance, Derek James Lura

USF Tampa Graduate Theses and Dissertations

This work focuses on the use of 3D motion capture data to create and optimize a robotic human body model (RHBM) to predict the inverse kinematics of the upper body. The RHBM is a 25 degrees of freedom (DoFs) upper body model with subject specific kinematic parameters. The model was developed to predict the inverse kinematics of the upper body in the simulation of a virtual person, including persons with functional limitations such as a transradial or transhumeral amputation. Motion data were collected from 14 subjects: 10 non-amputees control subjects, 1 person with a transradial amputation, and 3 persons with …

Water Quality Monitoring In A Pilot Marine Integrated Aquaculture System, Alex W. Kruglick

USF Tampa Graduate Theses and Dissertations

Land based aquaculture systems are growing in popularity, and there is a drive to improve the efficiency of their energy and water systems for both economic and environmental benefit. Recirculating Aquaculture Systems (RAS) generally refer to systems that recycle their wastewater streams to be more or less a zero discharge facility. A more sustainable approach is emerging in the form of Integrated Aquaculture Systems (IAS) where maximum utility is realized from the various components so that fresh water use is reduced, water quality is improved, energy use is reduced, and new markets are created or fulfilled. Under a grant from …

Tales Of Empire: Orientalism In Nineteenth-Century Children's Literature, Brittany Renee Griffin

USF Tampa Graduate Theses and Dissertations

Children's literature often does not hold the same weight in the studies of a culture as its big brother, the novel. However, as children's literature is written by adults, to convey information which is important for a child to learn in order to be a functioning member of that society, it can be analyzed in the same way novels are, to provide insight into the broad sweeping issues that concerned the adults of that era. Nineteenth-century British children's literature in particular reveals the deep-seated preoccupation the British Empire had with its eastern colonies, and shows how England's relationship to those …

Matrix Metalloproteinase Genes Are Transcriptionally Regulated By E2f Transcription Factors: A Link Between Cell Cycle Control And Metastatic Progression, Jacqueline Lea Johnson

USF Tampa Graduate Theses and Dissertations

The RbµE2F transcriptional regulatory pathway plays a critical role in the cell cycle. Rb is inactivated through multiple waves of phosphorylation, mediated mainly by cyclin D and cyclin E associated kinases. Once Rb is inactivated, cells can enter Sµphase. Collectively, three Rb family members and ten E2F proteins coordinate every additional stage of the cell cycle, from quiescence to mitosis. However the RbµE2F pathway is frequently altered in cancer. Aside from cell proliferation, the RbµE2F pathway regulates other essential cellular processes including apoptosis, cell differentiation, angiogenesis and DNA damage repair pathways, but its role in invasion and cancer progression is …

A Ctd Biotag For Mid-Sized Marine Predators, Heather Broadbent

USF Tampa Graduate Theses and Dissertations

Biologging tools for investigating the study of fine-scale linkages between animal behavior and the physical microstructure of the marine habitat are technically limited by substantial size, high cost or low sensor resolution. However, recent advances in electronic technologies and process techniques present attractive alternatives to current tag designs. Motivated by the need for a low-cost, compact CTD biotag for medium-sized marine animals, the University of South Florida Center for Ocean Technology developed a multi-sensor biotag for quantitative measurements of ocean salinity. This dissertation describes the development and performance of a novel CTD biotag used for animal-borne measurements of the physical …

The Role Of Tumor Suppressors, Ship And Rb, In Immune Suppressive Cells, Michelle Marie Collazo Ruiz

USF Tampa Graduate Theses and Dissertations

Regulatory T cells (Tregs) and myeloid-derived suppressor cells (MDSC) have been extensively studied in the past 30-40 years. Their potent suppressive capacity shown in several pathological and clinical settings, such as cancer and transplantation, has made it evident that better understanding their development and function is critical.

Specifically, Tregs play a pivotal role in preventing autoimmunity, graft-versus-host disease (GvHD), and organ graft rejection. We previously demonstrated that germline or induced SH2 domain-containing inositol 5-phosphatase (SHIP) deficiency in the host abrogates GvHD. Here we show that SHIP-deficiency promotes an increase of FoxP3+ cells in both the CD4+CD25+ and the CD4+CD25- T …

The Effects Of Oxygen On The Electrophysiology Of Co2/H+-Chemosensitive And -Insensitive Neurons Of The Solitary Complex Of The Rat, Michael Patrick Matott

USF Tampa Graduate Theses and Dissertations

This study tested the hypothesis that decreasing the control O₂ level from 95% to 40% (5% CO₂ + 55% N₂) maintains viability in caudal solitary complex (cSC) neurons in transverse slices (~300-400ꝳ) prepared from neonatal rat (P2-22) maintained at 32-34°C. The underlying rationale is to reduce exposure to redox and nitrosative stimuli generated during several hours of exposure to 95% O₂ that produces a tissue O₂ tension throughout the slice which is in excess of 203 kPa (2.0 atmospheres absolute,ATA) oxygen. Whole cell recordings of cSC neurons maintained in 40% O₂ exhibited spontaneous …

Ncr Vs Dro: Evaluation Of Effectiveness, Teacher Preference, And Fidelity Of Implementation, Jackie Courntey Lansdale

USF Tampa Graduate Theses and Dissertations

Abstract

Previous research has demonstrated that non-contingent reinforcement (NCR) and differential reinforcement of other behaviors (DRO) are effective procedures in reducing problem behavior of children both in and out of the classroom. However, few studies have assessed which procedure is most socially acceptable among teachers. In addition, studies have not recorded data on fidelity of implementation among teachers. A non-concurrent multiple baseline across teachers design was used to (a) demonstrate the effect of NCR and DRO on the problem behaviors of school aged children with no identified developmental disability, and (b) assess implementation fidelity of each procedure by the teacher. …

Influence Of Stress And Cytokinic Profiles On Cognitive Performance In Older Adults, Kerri S. Rawson

USF Tampa Graduate Theses and Dissertations

ABSTRACT

With aging, changes in the immune system, makes cognitive performance, and the prevalence of stressors can lead to poorer overall functioning. Within the immune system, a balance should exist between cytokines regulating Th1 and Th2 immune responses; however, age-related declines in the endocrine and immune systems can disrupt this equilibrium. Several studies report higher levels of Th1 associated cytokines in inflammatory conditions of the brain, whereas fewer studies remark on Th2 associated cytokines and cognitive functioning. Declining cognitive abilities are a common concern that accompanies advancing age and some research has suggested the prevalence and impact of stressors lead …

Development Of A Bi-Directional Electronics Platform For Advanced Neural Applications, Luca Abbati

USF Tampa Graduate Theses and Dissertations

This work presents a high-voltage, high-precision bi-directional multi-channel system capable of stimulating neural activity through bi-phasic pulses of amplitude up to ∓50 V while recording very low-voltage responses as low as tens of microvolts. Most of the systems reported from the scientific community possess at least one of the following common limitations: low stimulation voltages, low gain capabilities, or insufficient bandwidth to acquire a wide range of different neural activities.

While systems can be found that present remarkable capabilities in one or more specific areas, a versatile system that performs over all these aspects is missing. Moreover, as many novel …

"Are We Supposed To Be The Guy On The Horse?" A Case Study On The Use Of Political Cartoons In The American History Classroom, James Manuel Duran

USF Tampa Graduate Theses and Dissertations

Recent reports on the media saturation experienced by the twenty-first century student have brought about an increased interest in focusing attention on the issue of visual literacy in today's schools. Concepts such as instructional personalization, where approaches to curriculum design and instruction are created to concentrate on the individual strengths of the learner, have been promoted by some as a path to improving overall student performance. Many believe that the content of the Social Studies classroom easily lends itself to a visually stimulating approach and as such is an ideal laboratory to test hypotheses on such an approach. This study …

Consequences Of Kleptoplasty On The Distribution, Ecology, And Behavior Of The Sacoglossan Sea Slug, Elysia Clarki, Michael Louis Middlebrooks

USF Tampa Graduate Theses and Dissertations

The sacoglossan sea slug Elysia clarki is able to photosynthesize for three to four months using chloroplasts sequestered from its algal food sources. Furthermore, the slug is able to store multiple chloroplasts from different algal species within the same cell. This research, consisting of several related studies, explores the role that provision of organic nutrients via photosynthesis plays in the biology of the slug. The first chapter demonstrates that, under conditions of starvation, photosynthetic activity in E. clarki remains fully functional for one month after which it then declines. During the first month of starvation the slug exhibits similar feeding …

Characterization Of The Lone Extracytoplasmic Function Sigma Factor, Ós, And Its Role In The Staphylococcus Aureus Virulence And Stress Responses, Halie Kay Miller

USF Tampa Graduate Theses and Dissertations

Previously our laboratory had identified a novel component of the Staphylococcus aureus regulatory network, an extracytoplasmic function ó factor, óS, involved in stress response and disease causation. Here we present additional characterization of óS, demonstrating a role for it in protection against DNA damage, cell wall disruption and interaction with components of the innate immune system. Promoter mapping reveals the existence of four unique sigS start sites, one of which appears to be subject to auto-regulation. Transcriptional profiling revealed that sigS expression remains low in a number of S. aureus wild-types, but is upregulated in the highly mutated strain RN4220. …

Neuron To Microglia Communication; A Study Of Cx3cl1 Signaling Implications In Cognitive Function, Inflammation, And Neurodegeneration, Joshua M. Morganti

USF Tampa Graduate Theses and Dissertations

As a consequence of aging, the brain is subject to chronic neuroinflammatory conditions. The resident immune cells of the brain, microglia, act similarly to peripheral macrophages to protect the brain from insults, infection, and physical trauma. However, without proper regulation of their respective host defense mechanisms, these actions can become neurotoxic. In the healthy brain neurons have several signaling systems that directly interact with microglia in order to maintain a calming influence upon their actions, one of particular interest is the chemokine CX3CL1. This chemokine is found predominantly on neurons, while its cognate receptor CX3CR1 is found exclusively on microglia. …

Modification Of Trophic Links Between An Omnivore And Macroinfaunal Prey From Sandy Beaches Of Differing Physical Regimes, Kristina Joan Morrow

USF Tampa Graduate Theses and Dissertations

Sandy beach ecosystems have been studied worldwide; however, ecological data are sparse for the extensive barrier islands of Florida. Accordingly, I investigated the feeding patterns of the ghost crab (Ocypode quadrata), a dominant omnivore inhabiting beaches along the Floridian coast. Density data was collected for ghost crabs and swash macroinfaunal prey. In addition, I utilized stable isotopes in conjunction with the mixing models IsoSource and SIAR to characterize diets of ghost crabs across three barrier islands in spring and summer 2011. Results showed that ghost crabs at Cayo Costa feed primarily on swash macroinfauna, while those from Anclote Key shifted …