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Asbestos Exposure In The Research Laboratory, Ediberto D. Garcia

USF Tampa Graduate Theses and Dissertations

Introduction: Asbestos has been employed in a myriad of industrial applications for more than a century. Of the 181 Mt of asbestos produced worldwide, over 31 Mt was consumed in the U.S. The production and consumption of this mineral eventually was found to cause asbestosis, lung cancer and mesothelioma. While the brunt of the human exposure to asbestos occurred in insulation work, many more workers in other jobs were expose to asbestos fibers. Very little is known about the potential exposure to asbestos in research labs.

Goal: In order to investigate potential research lab work exposures, we simulated lab work …

Immobilization And Characterization Of Physisorbed Antibody Films Using Pneumatic Spray As Deposition Technique, Jhon J. Figueroa

USF Tampa Graduate Theses and Dissertations

The immobilization of antibodies on silica surfaces has been a wide and common practice via cross-linking with the formation of covalent bonds between surface and antibody. The formation of antibody thin films on solid surfaces using pneumatic spray (PS) as the deposition technique and the analysis of the surface morphology of these films were investigated during this study. The pneumatic spray method was compared with the covalent bonding method Avidin-Biotin Bridge (ABB). The intensities and capture efficiency tests showed similar results for both techniques with a lower signal-to-noise ratio (SNR) for the PS deposited films. Specificity tests suggested that the …

Optical Investigations Of Neurohypophysial Excitability And Amyloid Fibril Formation, Joseph Leo Foley

USF Tampa Graduate Theses and Dissertations

This dissertation describes the work done on two distinct projects. In the first part I sought to unravel the mechanisms that underlie the activity-dependent modulation of response in the excitation-secretion coupling of the neurohypophysis. In the second part, I optically monitored and analyzed the secondary structure changes accompanying amyloid fibril formation along multiple pathways, under both denaturing and near-physiological conditions.

Neuronal plasticity plays an important role in regulating various biological systems by modulating release of hormones or neurotransmitters. The changing response to the same stimulus, depending on the context and previous stimulation events, is also the basis of learning and …

An(Other) Rhetoric: Rhetoric, Ethics, And The Rhetorical Tradition, Kathleen Sandell Hardesty

USF Tampa Graduate Theses and Dissertations

Rhetoric as a discipline is still touched by the shadow of ancient Greece. Rhetoric was defined famously by Aristotle as the "available means of persuasion," codified into five canons in classical Rome, and has since been a central part of Western education to train speakers and writers to effectively move their audiences. However, particularly beginning in the mid-20th Century, the discipline's understanding of rhetoric as a means of persuasion (or even manipulation) passed down from our ancient roots began to shift to a sense of rhetoric as matters of ethics and a concern for the other. It begs the question: …

Investigation Of Condition Effects On Batch Fecundity Of The Common Snook, Centropomus Undecimalis, In Tampa Bay, Florida, Catherine Michelle Bruger Hayslip

USF Tampa Graduate Theses and Dissertations

Common snook, Centropomus undecimalis, is a popular game fish species that occurs throughout central and southern Florida, yet many questions remain unanswered regarding their reproductive strategies, including estimates of fecundity with relation to size, age, and condition of the female. These relationships are critical to improving stock assessments and fundamental to estimating spawning potential ratio, the measure by which snook are managed. Spawning snook were collected from a known spawning site, Rattlesnake Key, Tampa Bay, Florida, during three consecutive spawning seasons (2009-2011) and batch fecundity for snook was reported for the first time. Of the 43 females suitable for fecundity …

Metabolism In Corals From Antarctica, The Deep-Sea, And The Shallow Subtropics: Contrasts In Temperature, Depth, And Light, Lara Henry

USF Tampa Graduate Theses and Dissertations

Coral habitats span the range from tropical to polar, extremely shallow to thousands of meters deep. The differences in light and temperature experienced in these varied habitats likely affect the metabolic rates of the corals residing there. The metabolism of three coral species from different habitats have been examined to elucidate the effects of these environmental parameters on metabolism, an under-studied aspect of coral biology. For all three species, measurements of oxygen uptake, ammonium excretion, and activity of the enzymes lactate dehydrogenase (LDH), malate dehydrogenase (MDH), and citrate synthase (CS) were used to characterize their metabolism. Off Florida's Gulf coast, …

World Heritage Status, Governance And Perception In The Pitons Management Area, St.Lucia, Vernice Camilla Hippolyte

USF Tampa Graduate Theses and Dissertations

There are currently 962 geographic sites in the world that have been classified as World Heritage. World Heritage is a unique concept, privy to and defined by UNESCO-- the United Nations, Educational, Scientific and Cultural organization, one of the specialized agencies and autonomous organizations established within the UN-United Nations system. World Heritage is governed by an international treaty called the Convention Concerning the Protection of the World Cultural and Natural Heritage, adopted by UNESCO in 1972 (The `Convention'). The inscription of a World Heritage Site or designation of World Heritage Status is highly coveted and considered in UNESCO parlance to …

Climate, Neo-Spinozism, And The Ecological Worldview, Nancy M. Kettle

USF Tampa Graduate Theses and Dissertations

The global community faces ecological problems with the natural environment and cultural impediments to solving them. Natural systems are constantly changing and so are cultural practices. Humans need to address both: the interaction between those dynamic systems, the natural and cultural, because what happens in one system changes things in the other. The changes to the ecosystems are rapid and sometimes irreversible while dealing with them has been inadequate. Environmental movements, including deep ecology, have been at the forefront of the efforts to engage the public, various groups, politicians, and world governments to address environmental problems on a coordinated large …

Unearthing Augusta: Landscapes Of Royalization On Roatan Island, Honduras, Lorena Diane Mihok

USF Tampa Graduate Theses and Dissertations

In 1742, the settlement of Augusta was established as an outpost of English royalization on Roatán Island, Honduras. This military camp housed a mix of English soldiers, English colonists, and local indigenous Miskitu peoples. While the settlement was occupied for only a brief span of seven years, the material record of the community provides insight into Miskitu-English interactions during the royalization process. Royalization encompassed strategies deployed by the English Crown to bring about loyalty to the state. In this dissertation, I discuss the concept of royalization from an agent-centered perspective to consider the intentions behind the occupants' usage of objects …

Synthesis Of Titanium Dioxide Photocatalyst With The Aid Of Supercritical Fluids, Haitao Li

USF Tampa Graduate Theses and Dissertations

Titanium Dioxide (TiO2) emerged as one of the most popular photocatalysts since 1970's. However, its photocatalytic activity requires UV irradiation due to its large band gap unless further functionalization or modifications are performed. Furthermore, recovery issue has always been a major drawback, if the more effective form nano particles are utilized.

The key objectives of this research were synthesizing new TiO2 based photocatalyst systems that are effective with both the UV and the visible light while utilizing novel superior environmentally friendly techniques enabling development of nano-structured photocatalysts that can be easily recovered. In this dissertation research, highly porous nano-structured TiO2/WO3/Fe3+ …

Persistent Infection With Human Herpesvirus-6 In Patients With An Inherited Form Of The Virus: A Newly Described Disease, Shara Pantry

USF Tampa Graduate Theses and Dissertations

Human Herpesvirus 6A (HHV-6A) and 6B (HHV-6B) are ubiquitous betaherpesviruses. Both viruses are associated with a variety of adult disorders including neurological disorder, such as multiple sclerosis and chronic fatigue syndrome. HHV-6 viruses are capable of establishing latency by integration into the telomeres of the host chromosome and are transmitted in a Mendelian manner in approximately one percent of the population. To date little is known about the immunological and neurological consequences of HHV-6 inheritance. This study focused on a unique population of individuals that inherited HHV-6 and present with chronic fatigue-like symptoms, including hypersomnia, generalized fatigue, headache, and short …

Relative Survival Of Gags Mycteroperca Microlepis Released Within A Recreational Hook-And-Line Fishery: Application Of The Cox Regression Model To Control For Heterogeneity In A Large-Scale Mark-Recapture Study, Beverly J. Sauls

USF Tampa Graduate Theses and Dissertations

The objectives of this study were to measure injuries and impairments directly observed from gags Mycteroperca microlepis caught and released within a large-scale recreational fishery, develop methods that may be used to rapidly assess the condition of reef fish discards, and estimate the total portion of discards in the fishery that suffer latent mortality. Fishery observers were placed on for-hire charter and headboat vessels operating in the Gulf of Mexico from June 2009 through December 2012 to directly observe reef fishes as they were caught by recreational anglers fishing with hook-and-line gear. Fish that were not retained by anglers were …

Social Determinants Of Racial And Ethnic Disparities In Perinatal Morbidity: Social Origins Of Perinatal Health Study, Abraham A. Salinas-Miranda

USF Tampa Graduate Theses and Dissertations

BACKGROUND: The social causation of preterm birth remains elusive, without an adequate explanatory framework. Thus, this study proposed and evaluated a conceptual model of the social determinants of perinatal health for the understanding of perinatal health disparities.

METHODS: A prospective cohort study was conducted with pregnant women between 20 and 35 weeks gestation who were participating in two Healthy Start programs in Central Florida, from July 2011-August 2013. Perinatal health was operationalized based on gestational age, birth weight, and healthy start infant risk screen score. The predictors were: early life adversity, social position, maternal health-related quality of life, maternal stress, …

Fate Of Polybrominated Diphenyl Ethers During Wastewater Treatment Process Producing Reclaimed Water, Kristy Siegel

USF Tampa Graduate Theses and Dissertations

Polybrominated diphenyl ethers (PBDEs), flame retardants, have been applied to consumer goods, such as furniture, electrical devices, textiles, and appliances for decades. Due to their physico-chemical properties, PBDEs are semi-volatile and easily leach off the consumer good during aging, stress, or normal wear and tear of the good. Once airborne, they pose an environmental health threat because they can adsorb onto dust particles, soil, or other particulates that can be inhaled, ingested, or come into contact with the dermal layer. Additionally, PBDEs have a molecular structure similar to other persistent organic pollutants, such as polychlorinated biphenyls and polychlorinated dibenzo-p-dioxins and …

Development Of An Investigator-Designed Questionnaire Concerning Childbirth Delivery Options Based On The Theory Of Planned Behavior, Chun-Yi Tai

USF Tampa Graduate Theses and Dissertations

This study responds to the globally increasing rate of caesarean section, and specifically to the very high rate of elective caesarean section among Taiwanese mothers as evidence suggests that such elective caesareans pose potential health risks for mothers and babies. The purpose of this study was to develop and evaluate a multi-component instrument based on the theory of planned behavior (TPB) to better understand Taiwanese pregnant women's decisions regarding their childbirth delivery options (spontaneous vaginal delivery or elective caesarean section). The study was a four-phased mixed method design. First, the TPB guided item development and instrument drafting. Second, pretesting and …

Predation, Competition, And Nutrient Levels Affect The Survival Of Escherichia Coli, Enterococci And Enteric Pathogens In Aquatic Habitats, Pauline Wanjugi

USF Tampa Graduate Theses and Dissertations

Fecal indicator bacteria (FIB) such as Escherichia coli and enterococci are used to assess microbiological water quality in recreational waters worldwide. FIB are used with the assumption that their presence correlates with that of fecal-associated pathogens in recreational waters. In aquatic habitats, several factors can interfere with the predictive relationship between FIB and pathogens including extended survival of FIB in secondary habitats such as sediment, vegetation and sand. Furthermore, many biotic (e.g. predation from bacterivorous protozoa and competition from indigenous bacteria) and abiotic factors (e.g. temperature, salinity, ultraviolet (UV) light irradiation, and nutrient availability) can influence the fate of FIB …

Stable Isotopes And Trace Elements In Tooth Enamel Bioapatite: Effects Of Diagenesis And Pretreatment On Primary Paleoecological Information, Jessica Norman Wilson

USF Tampa Graduate Theses and Dissertations

The geochemical analysis of bioapatite in vertebrate skeletal tissues is an important tool used to obtain ecological information from fossil animals. An important consideration when conducting stable isotope and trace element analyses is obtaining biogenic information that has been unaffected by diagenetic processes.

A two-step pretreatment procedure is commonly used remove diagenetically altered material by removing organic material, via an oxidation reaction with H2O2 or NaOCl, and secondary carbonate, via dissolution in dilute acetic acid, from bioapatite. While much work has been done to determine the efficacy of the pretreatment process, little research has been conducted to determine the potential …

Effects Of Virtual Reality On The Cognitive Memory And Handgun Accuracy Development Of Law Enforcement Neophytes, Richard A. Wright

USF Tampa Graduate Theses and Dissertations

Abstract

The purpose of this research was to investigate the effects of virtual reality training on the development of cognitive memory and handgun accuracy by law enforcement neophytes. One hundred and six academy students from 6 different academy classes were divided into two groups, experimental and control. The experimental group was exposed to virtual reality training for a period of 8 hours. The control group was exposed to the traditional, non-interactive training that occurred on a gun range, also for a period of 8 hours. After exposing the groups to their respective training, a counter-balance technique was utilized to expose …

Improving Implementation Of A Regional In-Line Chlorinator In Rural Panama Through Development Of A Regionally Appropriate Field Guide, Benjamin A. Yoakum

USF Tampa Graduate Theses and Dissertations

Access to safe drinking water has a direct effect on improving human health and their quality of life. One country still struggling with providing access to safe drinking water to all of its population is Panama. Panama's largest indigenous group, the Ngöbe people, is disproportionately affected by lack of access to safe drinking water. One way Panama's Ministry of Health (MINSA) is attempting to increase access to safe drinking water to the Ngöbe people is by disinfecting the water already captured by rural gravity fed water systems constructed within in the Ngöbe-Bugle reservation. This is accomplished using an in-line chlorinator …

Analysis Of Variable Effects On Presence Of Cryptosporidium Oocysts And Giardia Cysts In Effluent Water From Wastewater Treatment Utilities In Florida From 1998 To 2010, Katherine Jane Barkan

USF Tampa Graduate Theses and Dissertations

The concern of a Cryptosporidium or Giardia waterborne outbreak due to treated wastewater has had water treatment utilities using some of the highest water cleansing technologies available. Cryptosporidiosis and Giardiasis are severe diarrheal diseases which can lead to death, thus it is important that appropriate steps are taken to assure these parasites are not present in the effluent of treated wastewater. This study examined the results of 863 assays for Giardia and Cryptosporidium on the effluent of wastewater treatment facilities and found that county of collection, watershed of collection, and laboratory analyzing the sample have the most significant impact on …

Reproductive Biology Of The Southern Dwarf Siren, Pseudobranchus Axanthus, In Southern Florida, Zachary Cole Adcock

USF Tampa Graduate Theses and Dissertations

The salamander family Sirenidae is composed of two extant genera, Siren and Pseudobranchus, each with two known species. Several questions regarding Sirenidae life history persist, and much of the available life history data for Pseudobranchus are attributed to studies of P. axanthus in northern Florida. Information on the reproductive biology of P. axanthus is limited, and historical references often suggest contradictory results. This study was undertaken to clarify information and expand on the limited data regarding P. axanthus reproductive biology, specifically for southern Florida populations. The study population was most likely the P. a. belli subspecies. P. axanthus in southern …

Assessment Of Student Achievement In Introductory Physical Geology: A Three -Year Study On Delivery Method And Term Length, Marianne O'Neal Caldwell

USF Tampa Graduate Theses and Dissertations

Physical Geology is a popular general education course at Hillsborough Community College (HCC) as at many other colleges and universities. Unlike many science courses, most students taking Physical Geology are not majoring in a STEM (Science, Technology, Engineering, and Mathematics) discipline. Typically most students enrolled in Physical Geology are majoring in business, education, or pursuing a general A.A degree for transfer to a four-year university. The class is likely to be one of the few, if not the only, physical science classes that many of these students will take in their academic career. Therefore, this class takes on increased importance, …

Production Of Bioactive Secondary Metabolites By Florida Harmful Bloom Dinoflagellates Karenia Brevis And Pyrodinium Bahamense, Cheska Burleson

USF Tampa Graduate Theses and Dissertations

Despite the critical role algae serve as primary producers, increases or accumulation of certain algae may result in Harmful Algal Blooms (HABs). Algal toxins from these blooms contribute significantly to incidences of food borne illness, and evidence suggests HABs are expanding in frequency and distribution. Mitigation of these HABs without knowledge of the ecological purpose and biochemical regulation of their toxins is highly unlikely. The production, function, and potential of secondary metabolites produced by the dinoflagellates Karenia brevis and Pyrodinium bahamense, were investigated.

Brevetoxins were demonstrated by two different methods to localize within the cytosol of Karenia brevis. …

The Impact Of Arranging Music For The Large Ensemble On The Teacher: A Phenomenological Exploration, James Teodor Lindroth

USF Tampa Graduate Theses and Dissertations

Abstract

The purpose of this study was to examine the phenomenon of what arranging music in the large instrumental ensemble music setting came to mean to the teachers doing the arranging. Four secondary music teachers (N=4) were asked to create a musical arrangement for one of their school ensembles. Over a period of seven weeks, participants rehearsed their arrangement with their students. This study was guided by research on creative identity, the self, and various identity theories from the field of the social sciences. Data were collected by way of in depth semi-constructed interviews, field observations, and journals; and were …

Availability And Quality Of Vegetation Affects Reproduction Of The Gopher Tortoise (Gopherus Polyphemus) In Improved Pastures, Anna Louise Hathaway

USF Tampa Graduate Theses and Dissertations

As part of a state-funded Gopher Tortoise (Gopherus polyphemus Daudin) translocation project, I monitored actively grazed improved pastures to determine if they could serve as suitable recipient sites for the threatened Gopher Tortoise displaced by human development. For cattle ranches to be considered suitable recipient sites females must be able to acquire sufficient energy to produce a clutch of viable eggs, and sufficiently high quality vegetation must be available to support juvenile recruitment into the population. Vegetation surveys were conducted to determine the composition and percent cover of plant species, especially those containing high amounts of nutrients, specifically nitrogen. Resident …

Exploring Picture Word Priming Effects In Healthy Aging Adults Using Event Related Potentials, Sasha C. Christopher

USF Tampa Graduate Theses and Dissertations

Objective: The aim of the present study was to investigate the mechanics with which older adults activate and access different subdomains of their mental lexicons during word retrieval for picture naming.

Method: Data were analyzed for 12 aging, native English speakers who performed a picture-word priming task. The auditory probe words were presented in the following conditions in relation to the picture stimuli: Identically related, strongly semantically related, weakly semantically related, strongly phonologically related, weakly phonologically related, semantically related to the strong phonological relative of the target picture label, or phonologically-related to the strong semantic relative of the target picture …

Butterbeer, Cauldron Cakes, And Fizzing Whizzbees: Food In J.K. Rowling's Harry Potter Series, Leisa Anne Clark

USF Tampa Graduate Theses and Dissertations

ABSTRACTThis thesis situates the Harry Potter books into the greater body of food studies and into the extant children's literary tradition through an examination of how food can be used to understand cultural identity. Food is a biological need, but because we have created social rules and rituals around food consumption and sharing, there is more to eating than simple nutritional value. The Harry Potter series is as much about overcoming childhood adversity, and good versus evil, as it is about magic, and food in the Harry Potter series is both abundant and relevant to the narrative, context, and themes …

The Role And Regulation Of Nsars: A Cell-Envelope Stress Sensing Two-Component System In Staphylococcus Aureus, Stacey Lynn Kolar

USF Tampa Graduate Theses and Dissertations

Abstract

S. aureus has 16 predicted two-component systems (TCS) that respond to a range of environmental stimuli, and allow for adaptation to stresses. Of these 16, three have no known function, and are not homologous to any other TCS found in closely related organisms. NsaRS is one such element, and belongs to the intramembrane-sensing histidine kinase (IM-HK) family, which is conserved within the Firmicutes. The regulators are defined by a small sensing domain within their histidine kinase, suggesting that they do not sense external signals, but stress in or at the membrane. Our characterization of NsaRS in this work reveals …

A Study Of The Eastern Oyster, Crassostrea Virginica, In Tampa Bay: Effects Of Perkinsus Marinus On Reproduction And Condition, Bridgit Melora Mccrickard

USF Tampa Graduate Theses and Dissertations

Abstract

Five sites in Tampa Bay, Florida, were sampled monthly from February 2006 to January 2007. These sites were located at the mouth of the Alafia River, in an inlet of Cross Bayou, on the easement of Gandy Bridge, near mangrove in Manatee County, and an inlet of Salt Creek, off Bayboro Harbor. Standard methods were used to determine shell height and Condition Index (CI). Intensity and prevalence of Perkinsus marinus were analyzed using Ray's Thioglycollate medium test, while Haplosporidium nelsoni was studied by histological examination. Histological methods were also used to determine sex ratios, reproductive phases, and egg diameters. …

Franz Marc As An Ethologist, Jean Carey

USF Tampa Graduate Theses and Dissertations

Keywords: Animalisierung, Einfühlung, Ethology,

Expressionism, Painting, German Modernism

In this study I deploy the perspective of ethology to examine Franz Marc's paintings of animals. To perceive animals ethologically means acknowledging that animals feel, think, experience, and imagine the world. Ethology has come to include interpretive pursuits as well as traditional field studies, and as I show, Marc's practice encompassed both aspects of this evolving discipline. To establish the presence of ethology in the humanities I give a "case study" of what I call "retroactive ethology" in the work of J.M. Coetzee. I present an account of Marc's deep …