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Role Of The Glutamine-Polyamine-Hypusine Axis In Cd8+ T Cell Fate And Function, Aya Gamal Aly Sadek Elmarsafawi

USF Tampa Graduate Theses and Dissertations

T cells are critical effectors of the immune response against pathogens and malignancies and, given the recent success of T-cell immunotherapies in cancer, there is now an urgent need to enhance the efficacy and durability of T-cell immune responses to improve anti-tumor immunity. Formation of memory T cells is a critical step of a successful immune response as it ensures maintenance of long-term immunity and protection from re-exposure to foreign antigens. Notably, metabolism regulates the fate and function of T cells and is a powerful tool to modulate T-cell activity and memory differentiation. Accordingly, metabolic interventions to augment T-cell memory …

In Silico And In Vitro Drug Discovery Strategies And The Chemical Investigation Of Endophytic Fungi, Sarah Grace Dietrick

USF Tampa Graduate Theses and Dissertations

The biodiversity of our global marine ecosystems provides substantial treasure to discover: chemistry. Marine organisms that live in proximity to one another use their unique chemistry to compete with one another and thrive in their environment. Benthic invertebrates, specifically, that lack physical means of protection will produce secondary metabolites as a means of defense from their surroundings and predators. The compounds which they produce are ordinarily species-specific, highly diverse biomolecules. Biosynthetic processes within an organism construct these secondary metabolites from fragments of primary metabolites (amino acids, fatty acids, sugars, and so on). Their roles in protection have antibiotic, toxic, and …

Synthetic Studies Of Potential New Ketogenic Molecules, Mohammad Nazmus Sakib

USF Tampa Graduate Theses and Dissertations

The ketogenic diet has gained popularity in the past few decades among people and physicians due to its various health benefits. A significant amount of research has been done for the modification of the original ketogenic diet and investigation of its potential health benefits. However, synthesis of novel ketogenic compounds as a dietary supplement has not received the same level of attention. That is the primary objective of this research project. The first chapter of this manuscript describes the synthesis of various short and medium chain esters of different polyols that have been done so far, and areas of their …

The Functions Of Oligodendrocyte Lineage-Derived Laminins Under Homeostatic And Intracerebral Hemorrhagic Stroke Conditions, Minkyung Kang

USF Tampa Graduate Theses and Dissertations

Oligodendrocyte lineage is a glial cell population in the central nervous system. The lineage starts with oligodendrocyte precursor cells, which differentiate and mature into mature oligodendrocytes. Myelination, the major function of mature oligodendrocytes, plays a significant role for normal function of the central nervous system, and its dysfunction leads to many demyelinating diseases, such as multiple sclerosis. During the lineage progression, an extracellular matrix protein, laminin, actively regulates the cells' biology, including cell proliferation, differentiation, and myelination. However, the cellular sources of the laminin that controls oligodendrocyte lineage cells remain largely unknown. Interestingly, previous studies have shown that oligodendrocyte lineage …

Investigating Mobility In The Northern Balkans During The Fifth Millenium Bc Through Strontium Analysis Of Human Tooth Enamel, Aurélien Tafani

USF Tampa Graduate Theses and Dissertations

This research investigates how individual mobility among ancient populations in Romania during the Eneolithic, between 5000 and 4000 BC, may be related to the spread of the material culture known as Kodjadermen-Gumelniţa-Karonovo VI (KGK VI) that extended over most of southern Romania and Bulgaria. This work seeks to combine results on individual mobility with other lines of archaeological evidence to provide a better understanding of the social context and kinship relationships that were in place when these events took place.

Strontium isotopic analyses were performed on 130 individuals from two sites located in the southern Romania: 49 from Sultana and …

Targeting Bet Proteins Downregulates Mir-33a To Promote Synergy With Pim Inhibitors In Cmml, Christopher T. Letson

USF Tampa Graduate Theses and Dissertations

Chronic Myelomonocytic Leukemia (CMML) is a rare myeloid malignancy with a dismal prognosis and no therapeutic options which are capable of altering the natural course of the disease. There remains a significant need for novel therapies that are able to meaningfully improve patient outcomes. In this study we explore the effectiveness of Bromodomain and Extra-Terminal domain protein inhibitor (BETi) combinations in CMML. Preclinical studies in myeloid neoplasms have demonstrated efficacy of BETi. However, BETi demonstrate poor single agent activity in clinical trials. Several studies suggest that combinations with other anti-cancer inhibitors may enhance the efficacy of BETi. To nominate BETi …

Coupling Chemical And Genomic Data Of Marine Sediment-Associated Bacteria For Metabolite Profiling, Stephanie P. Suarez

USF Tampa Graduate Theses and Dissertations

Marine sediment-associated bacteria house many new and exciting novel secondary metabolites. These metabolites can be tested for bioactivity against various types of cancer and fungal, bacterial, and viral infections. In this thesis, we investigated the combination of biosynthetic gene cluster information with mass spectra to perform a chemical profiling of sediment- associated bacteria. Furthermore, we utilized a scoring technique to provide an identification and confidence score to each annotated compound. The sediment was collected from east Arthur Harbor, Palmer Station, Antarctica, at depths of 20 ft and 60 ft. After plating on agar, 52 unique bacterial strains were isolated, with …

Integrated Periphyton Biofilters In Marine Recirculating Aquaculture Systems, Adam N. Bell

USF Tampa Graduate Theses and Dissertations

Marine aquaculture is a growing industry that has the potential to reduce eutrophication, overfishing, and loss of biodiversity. Recirculating aquaculture systems (RAS) are a type of aquaculture system that decreases discharge, raises biological control, and conserves water; however, RAS are challenged due to high energy cost, low biodiversity, water quality control, and waste nutrients. Periphyton biofilters are a new technology that allows for feed production, water quality control, microbial diversity, and resource recovery in marine aquaculture systems. The overarching question that this dissertation investigates is, how can periphyton biofilters be integrated into RAS to address the challenges of modern sustainable …

Brain Metastasis-Associated Fibroblasts Secrete Fucosylated Pvr/Cd155 That Induces Breast Cancer Invasion, Emma Adhikari

USF Tampa Graduate Theses and Dissertations

Breast cancer (BC) continues to be the most diagnosed cancer and the second leading cause of cancer deaths in women worldwide. High mortality, in most cancers, is due to metastasis; BC is the most common source of one of the deadliest forms of metastasis–brain metastasis (BM) in female. Patients with triple negative breast cancer (TNBC) exhibit the highest incidence of BM and poorest survival outcomes upon diagnosis with BM. The prognosis of breast cancer brain metastasis (BCBM) remains poor because the blood–brain barrier (BBB) prevents the uptake of drug substances, including chemotherapeutic agents, targeted agents, and toxins, from the brain. …

Deep Learning-Based Automatic Stereology For High- And Low-Magnification Images, Hunter Morera

USF Tampa Graduate Theses and Dissertations

Quantification of the true number of stained cells in specific brain regions is an important metric in many fields of biomedical research involving cell degeneration, cytotoxicology, cellular inflammation, and drug development for a wide range of neurological disorders and mental illnesses. Unbiased stereology is the current state-of-the-art method for collecting the cell count data from tissue sections. These studies require trained experts to manually focus through a z-stack of microscopy images and count (click) on a hundred or more cells per case, making this approach time consuming (~1 hour per case) and prone to human error (i.e., inter-rater variability). Thus, …

Booker Creek: Wet Season Water Quality Trends In A Florida Urban Stream, Alexandra M. Shostak

USF Tampa Graduate Theses and Dissertations

Booker Creek, a 1.9-mile stream with a 4.9-mi2 watershed, flows southeast through highly urbanized St. Petersburg, Florida, and empties into Bayboro Harbor, which connects with Tampa Bay. This study collected and analyzed water quality data for several parameters (TN, TP, nitrate-nitrite, ammonia, orthophosphate, chl-a, fecal coliform, dissolved oxygen, pH, turbidity, temperature, and salinity) every two weeks, at four different sites, during the 2022 wet season to characterize the current state and geographic variability of Booker Creek. Current creek sampling regimes, though consistent, do not sample at multiple sites along the creek. Urban streams do not display uniform water quality along …

“We Need To Figure Out Who We Are”: Reframing Manhood In An Online Discussion Forum, Tomas Sanjuan Jr.

USF Tampa Graduate Theses and Dissertations

In this thesis, I explore the potential of online communities in negotiating alternative forms of “doing” masculinity. I focus on the /r/bropill which is hosted on Reddit – home to thousands of active discussion forums called subreddits. I argue that the members of /r/bropill subreddit are attempting to redefine what it means to live your life not only as a man but as a “good man.” Using a purposive sample, I analyzed 24 discussions which totaled 1325 posts (n = 1325). I conducted a qualitative textual analysis of the original posts and comments inspired by grounded theory. My findings reveal …

Neuroendocrine Regulation Of Chemokine Receptor-Mediated Leukocyte Migration Via Heteromeric G Protein-Coupled Receptor Complexes, Garrett Enten

USF Tampa Graduate Theses and Dissertations

Neuroendocrine regulation of the innate immune system is a well-documentedphenomenon. The underlying mechanisms, however, are not well understood. Here we show that at least 20 members of the human chemokine receptor (CR) family heteromerize with one or more members of the α1-adrenergic receptor (AR) family and that all CRs, apart from CXCR1, heteromerize with arginine vasopressin receptor 1A (AVPR1A) in recombinant systems. These heteromeric complexes are detectable in human monocytes and the monocytic leukemia cell line THP-1. Though currently technical limitations prevent the direct visualization of endogenously expressed higher-order hetero-oligomeric receptor complexes, we provide evidence through functional analysis and proximity-based …

Water Chemistry Variability In Peacock Springs Cave System And Spring Basin: Impacts On Continuous Monitoring Of Karst Aquifers, Mahnoor Kamal

USF Tampa Graduate Theses and Dissertations

The Upper Floridan Aquifer (UFA) is a highly transmissive and heterogeneous karst aquifer that makes it acutely susceptible to pollution. Rapid and extensive water quality changes in nutrients at springs draining the UFA have prompted intensive monitoring at several priority springs, including continuous and discrete water quality sampling. Springs, however, are a discrete discharge point of an often-expansive phreatic cave network that can intersect different land uses and thus drain distinct geographic areas of a springshed. The extensive network contains convergent passages from deep and shallow aquifer regions and may contribute to varying water chemistry across passages resulting in water …

Affordances And Limitations Of Molecular Representations In General And Organic Chemistry, Ayesha Farheen

USF Tampa Graduate Theses and Dissertations

Molecular representations that show chemical bonding are ubiquitous in general and organic chemistry. These help in communicating chemistry concepts that are fundamental to understanding how and why molecules interact. The goal of this dissertation work is to offer an evidence-based report on the affordances and limitations of the variety of molecular representations by chemistry topic. The variety of representations investigated are chemical formula, Lewis dot structure, line-angle diagram, ball and stick, space filling, and electrostatic potential maps. Five studies were conducted with general and organic chemistry students and three key findings emerged. First, affordances and limitations of molecular representations are …

Wastewater Treatment Using Encapsulated Chabazite In Polyvinyl Alcohol-Sodium Alginate Hydrogel Biofilm Carriers, Susieanna Nevada Persaud

USF Tampa Graduate Theses and Dissertations

Nitrogen discharge from wastewater is a global issue and with increased urbanization as well as industrialization, it has become even more essential to develop more efficient wastewater treatment systems. The dominant form of nitrogen in wastewater is ammonium. Biological oxidation of ammonium to nitrite and nitrate is the first step in biological nitrogen removal processes. If left untreated, nitrogen can cause toxicity to aquatic life, unsafe changes in water quality, and eutrophication.

Biofilm carriers can help to improve the efficiency of traditional activated sludge wastewater treatment systems by reducing operating costs and reducing hydraulic retention time. Biofilm carriers also promote …

An Experimental Analysis Of The Effect Of Crisis Response Message Strategies On Consumer Emotions, Perceptual Beliefs And Intended Behavior, Valentina Ahumada

USF Tampa Graduate Theses and Dissertations

This study investigates the different dynamics of consumer responses to crisis communication strategies employed by businesses during challenging situations. Focusing on emotions, attitudes, and behavioral intentions, the research explores the impact of denial, diminish, and apology crisis messages on consumers. Drawing on a comprehensive analysis of a crisis scenario, the study illuminates the intricate relationship between these strategies and consumer emotions such as anger, disgust, sadness, and happiness. The findings reveal nuanced patterns, demonstrating that while denial messages evoke strong negative emotions and unfavorable attitudes, diminish messages, when perceived as deflecting blame, can lead to mixed emotional responses and ambiguous …

A Gis Workflow For Analyzing Spatiotemporal Variance In Food Pantry Access: A Case Study For Hillsborough County, Florida, Molly J. Darby

USF Tampa Graduate Theses and Dissertations

Researchers often use geographic information systems (GIS) to measure accessibility to food pantries, but the impact operating hours have on accessibility remains understudied. Unlike grocery stores or healthcare facilities, food pantries have irregular distribution schedules, therefore, measuring geographic accessibility alone potentially creates an inaccurate or inflated representation of access. This study proposes a GIS workflow for analyzing food pantry accessibility over space and time by considering hours of operation and location, allowing researchers to examine when and where access to food assistance is most limited over the span of a typical week. The method was applied to Hillsborough County, Florida …

Time-Series Analysis Of Coral Reef And Reef-Fish Biodiversity In The Florida Keys National Marine Sanctuary, Cara L. Estes

USF Tampa Graduate Theses and Dissertations

The general decline in coral-reef ecosystem health throughout the Caribbean between the 1970’s and late 1990’s seems to have slowed down or stopped, with only slight decreases in coral reef cover observed in the 2000’s through 2019. The earlier decline can be attributed to many different local and regional stressors that have acted together, and include naturally occurring (hurricanes, diseases) and anthropogenic pressures (increased sedimentation, fishing). These ecosystems provide a range of services to people, including food and protection from storms, with an annual benefit of billions of dollars. This has driven a desire to better understand these ecosystems. The …

Hurricanes And Tropical Storms’ Impact On Water Quality In Lake Okeechobee, Florida, Daniela Vasquez Diaz

USF Tampa Graduate Theses and Dissertations

Hurricanes are natural events with catastrophic outcomes, while climate change's effects on their intensity and frequency remain discussed. However, it is crucial to analyze these through models and statistics to understand their behavior and impact on the basins. Particularly in lakes on tropical coasts since disruptive events such as hurricanes tend to be highly affected. Lake Okeechobee is a unique lake located in Florida and one of the largest freshwater lakes. The lake and some of its significant stations are the locations chosen to perform the investigation. The main focus of the investigation is determining the effect that hurricanes have …

Analyzing Multi-Robot Leader-Follower Formations In Obstacle-Laden Environments, Zachary J. Hinnen

USF Tampa Graduate Theses and Dissertations

Observations in biological formation from nature likes flocks of birds, herds of mammals and packs of wolves have inspired the innovation of robotic architectures. This thesis presents an approach that aims to use robotic systems to mimic leader-follower behaviors in the navigation and formation of sparse and dense environments. The goal of this work is to extend and further analyze the original work of Weitzenfeld et al [3] to evaluate new swarm and pack based multi-robot architectures with the inclusion of obstacle avoidance and variations in group formations. The multiple robot architecture is based off a wolf pack with a …

Evaluating Video Feedback To Improve Esports Performance In Street Fighter V, Romel C. White

USF Tampa Graduate Theses and Dissertations

eSports is a newly rising field with rapidly increasing cultural validity. Despite the wide array of similarities between eSports and traditional sports, behavioral skill acquisition interventions have yet to be applied to video game skills. Feedback-based behavioral interventions in particular have been commonly used and implemented for effective skill acquisition for traditional sports. Although video feedback has been established as effective in conjunction with video modeling for traditional sports, further research is needed to validate its efficacy as a standalone intervention. In this study, the effects of video feedback were analyzed with three participants in a nonconcurrent multiple baseline across …

Quantifying The Effectiveness Of Night Vision Detection: A Comparative Study Of Visible Light, Night Vision, And Thermal Images, Feng Yeh

USF Tampa Graduate Theses and Dissertations

This research presents a comprehensive assessment of night vision goggles and thermal imagers, with a particular emphasis on their performance under a wide range of environmental and temporal conditions. Field testing of these devices was carried out across diverse settings, including daytime, nighttime, and different environmental temperatures. Key evaluation metrics, such as contrast, recognition distance, and image clarity, were employed to assess device performance.The findings reveal that while night vision goggles maintain consistent performance across various conditions, their image clarity and contrast are influenced by the recognition distance. Conversely, thermal imagers excel in cooler environments, successfully highlighting target objects, yet …

Chemical Analysis Of Metabolites From Mangrove Endophytic Fungus, Sefat E Munjerin

USF Tampa Graduate Theses and Dissertations

Natural products hold a significant place in drug discovery for their abundant and unique secondary metabolites. These secondary metabolites which encompass a range of organic compounds such as alkaloids, phenols, peptides, flavonoids, polyketides, and terpenoids, can easily be exploited for drug development. Endophytic fungi, residing harmoniously with its host, have emerged as generous producers of bioactive secondary metabolites, displaying efficacy against a wide array of human pathogens, including the challenging ESKAPE pathogens.

The research explores endophytic fungi and their prospective for drug discovery and development through the synthesis of distinctive secondary metabolites. Endophytic fungus, HM13-26C-2B, collected from Honeymoon Island, Florida …

3d Musculoskeletal Analysis Of The Rhea Americana Forelimb And Implications For The Evolution Of Avian Flight, Sarah Elizabeth Guzinski

USF Tampa Graduate Theses and Dissertations

To understand the evolutionary gain, and loss, of avian powered flight, attention must be paid to the wing morphology of species across these transitions. However, paleognaths, while being the most well-known flightless birds, have greatly understudied forelimbs. Additionally, current literature regarding their forelimb anatomy generally lacks a three-dimensional (3D), biomechanical approach. Among the flightless paleognaths, the rhea is notable in having the least reduced forelimb. Though not used for flight, the wings of rheas are used in other behaviors such as courtship and threat display, as well as nest defense. In this study, I investigate the forelimb anatomy of an …

Use Of Silica Dust And Lunar Simulants For Assessing Lunar Regolith Exposure, Layzamarie Irizarry-Colon

USF Tampa Graduate Theses and Dissertations

The utilization of the lunar regolith holds immense potential for future space exploration, but the potential health risks associated with lunar regolith are a significant concern. This literature review seeks to critically assess the use of silica and lunar simulants standards and permissible exposure limits (PELs) to evaluate the adverse health effects of lunar regolith. The literature review covers an extensive analysis of existing research, health risks, toxicological properties, and physical and chemical composition associated with silica, lunar simulants, and lunar regolith by using three main sources: regulations, scholarly articles, and Kennedy Space Center (KSC) discussion. This comprehensive analysis forms …

The Aging Workforce: How It Relates To Incident Rates Within A Distribution Warehouse And A Chemical Manufacturing Building, Elisabeth V. Jones

USF Tampa Graduate Theses and Dissertations

Over the past 20 years or so, the average age of the global population has slowly increased. This is due to low birth rates as well as increased life expectancy. With this global population aging, there has also been a shift in the global labor market causing a trend termed the “aging workforce.” However, with the aging workforce comes a new set of issues for the health and safety professional because they create new hazards. This is due mostly because of the body undergoing physical, psychological, and cognitive decline as someone ages which causes decreased capabilities. Thus, the purpose of …

Stable Isotope Analysis On Yellowfin And Blackfin Tuna Eye Lenses Reveals Life History Patterns In The Gulf Of Mexico, Kylee M. Rullo

USF Tampa Graduate Theses and Dissertations

Spatial geography, ontogenetic movement, and trophic patterns of mobile species are key elements of effective marine resource management. A number of methods are currently available for tracking movements of pelagic migratory species, including the use of conventional and electronic tags. However, tagging campaigns can take years to provide useful data, can be expensive, and only capture a portion of a fish’s lifetime. For this project, I used stable-isotopic ratios of nitrogen (δ15N) and carbon (δ13C) in metabolically inert, chronologically-layered fish-eye lenses to explore lifetime movement and trophic patterns of yellowfin tuna (Thunnus albacares) and blackfin tuna (Thunnus atlanticus) in the …

A Psychometric Analysis Of Natural Language Inference Using Transformer Language Models, Antonio Laverghetta Jr.

USF Tampa Graduate Theses and Dissertations

Large language models (LLMs) are poised to transform both academia and industry. But the excitement around these generative AIs has also been met with concern for the true extent of their capabilities. This dissertation helps to address these questions by examining the capabilities of LLMs using the tools of psychometrics. We focus on analyzing the capabilities of LLMs on the task of natural language inference (NLI), a foundational benchmark often used to evaluate new models. We demonstrate that LLMs can reliably predict the psychometric properties of NLI items were those items administered to humans. Through a series of experiments, we …

Spatial Structure Of Germination And Seed-Associated Fungi In Tropical Pioneer Seeds, Daniela Varon Garcia

USF Tampa Graduate Theses and Dissertations

Negative density-dependent regulation is the process by which host-specific natural enemies reduce the survival of seeds and seedlings of conspecifics near adult trees, favoring heterospecific recruitment, and ultimately increasing local diversity. Negative density-dependent regulation by fungal pathogens has been consistently reported to promote plant diversity in the tropics, even though they tend to be multi-host and host-specificity is rarely reported. Nevertheless, evidence suggests that host-generalist fungi can have host-specific effects that produce disproportionate negative effects on some plant species but not others, promoting regional diversity. However, such studies have not tested for both host-specificity and distance-dependent effects in fully replicated …