Digital Commons Network^™

Effect Of Solids Retention Time On The Denitrification Potential Of Anaerobically Digested Swine Waste, Maureen Njoki Kinyua

USF Tampa Graduate Theses and Dissertations

Three continuously stirred tank reactors (CSTR) were operated in semi continuous mode treating swine waste using anaerobic digestion. The reactors were used to test the effect of solid retention time (SRT) on CH4 yield, total ammonia nitrogen (TAN) concentrations, % volatile solids (VS), chemical oxygen demand (COD) and volatile fatty acids (VFA) removal, readily biodegradable COD concentration and the denitrification potential for the effluent in a biological nutrient removal (BNR) system. During Phase I of the study, the three reactors were operated at the same 28 day SRT for 16 weeks. SRTs were then changed during the 12 week Phase …

Monsters Under The Bed: An Analysis Of Torture Scenes In Three Pixar Films, Heidi Tilney Kramer

USF Tampa Graduate Theses and Dissertations

With background information on militarism, nationalism, and torture, this study analyzes Monsters, Inc., Toy Story 3, and The Incredibles, three Pixar films released from 2001 through 2010, for the ways in which the torture scenes are framed. These frames, state control, prisons, and 60s spy thrillers, invite laughter through intertextuality, while deflecting attention from torture of central characters in the films. The implications of this analysis are: these films present torture as deserved and normative; the tortured characters stand outside the frames of recognition for humanness; and they redefine children as threats and dangers. This study concludes that these ideologies …

Hispanic Acculturation And Social Media Engagement: A Uses And Gratifications Perspective, Roland Rahe Massa

USF Tampa Graduate Theses and Dissertations

Uses and gratifications theory posits that media use is an intentional and selective process by individuals in order to satisfy emotional needs. Basis this theory, the author conducted an exploratory study to compare social media engagement with four factors of acculturation among Hispanic Americans. These variables were also compared to the variables of education level and generation in order to test for a relationship. An online questionnaire captured data from a sample of Hispanic American participants (N = 85). Correlation analysis was performed, and results indicated that generation had a significant relationship with social media engagement and three out of …

Eschatology In A Secular Age: An Examination Of The Use Of Eschatology In The Philosophies Of Heidegger, Berdyaev And Blumenberg, John R. Lup, Jr.

USF Tampa Graduate Theses and Dissertations

The topic of eschatology is generally confined to the field of theology. However, the subject has influenced many other fields, such as politics and history. This dissertation examines the question why eschatology remained a topic of discussion within twentieth century philosophy. Concepts associated with eschatology, such as the end of time and the hope of a utopian age to come, remained largely background assumptions among intellectuals in the modern age. Martin Heidegger, Nicolai Berdyaev, and Hans Blumenberg, however, explicitly addressed the subject in their philosophies. The impetus of this study is Heidegger's statement, "Being itself is inherently eschatological," which indicates …

Pharmacological Versus Social Alcohol Expectancies: Making An Important Distinction Between The Anticipated Rewarding Effects Of Alcohol, Megan Victoria Mcmurray

USF Tampa Graduate Theses and Dissertations

Despite over 30 years of research investigating alcohol expectancies, they have never been examined in terms of the anticipated pharmacological versus social rewards resulting from alcohol consumption, and both appear to play a central role in drinking motivation and behavior. The purpose of this study was to develop a two-dimensional instrument designed to assess both the pharmacological alcohol expectancies of pleasurable, internal states that result from alcohol consumption, as well as the social expectancies that drinking alcohol will result in higher social status and increased effectiveness in social situations. This measure, called the Pharmacological and Social Alcohol Expectancy Scale (PSAES), …

The Perspectives Of Graduate Students With Visual Disabilities: A Heuristic Case Study, Luis Perez

USF Tampa Graduate Theses and Dissertations

The concept of liminality describes the experiences of individuals who live "between and betwixt" as a result of their indeterminate status in society. This concept seems appropriate to describe the experiences of people who live with vision loss, because we simultaneously belong to two social or cultural groups. On the one hand we must navigate the mainstream society in which we live day to day, which we are often able to do with the vision we have left. On the other hand, our disability sets us apart from that mainstream society. This idea of living in "between and betwixt" the …

Ecological Interactions Influencing Avicennia Germinans Propagule Dispersal And Seedling Establishment At Mangrove-Saltmarsh Boundaries, Jennifer Mcclain Peterson

USF Tampa Graduate Theses and Dissertations

Mangroves and saltmarshes are ecologically important coastal ecosystems; unfortunately, these low-lying coastal ecosystems are vulnerable to global climate change. As sea-levels rise, mangroves are expected to shift their distribution landward towards higher elevation sites that are occupied by other plants, including saltmarsh taxa. Therefore, mangrove recruits at the leading edge of expansion may interact with diverse assemblages of saltmarsh plants, and these interactions could influence the success of mangrove encroachment into higher tidal-elevation areas. The purpose of the research presented here was to investigate empirically the ecological interactions that may influence the recruitment of the black mangrove, Avicennia germinans, …

Investigation Of Hsf1 Interacting Partners Via A Genome-Wide Yeast Two-Hybrid Screen, Jamie Elizabeth Mendez

USF Tampa Graduate Theses and Dissertations

Heat shock factor 1 (HSF1) is the master transcriptional regulator of the heat shock response (HSR), an evolutionarily conserved cellular stress response. HSF1 promotes the expression of a variety of molecular chaperones that aid in restoring protein homeostasis upon exposure to proteoxic stress. However, all of the proteins responsible for regulating the HSR together with HSF1 are unknown. A genome-wide yeast two hybrid screen was performed to identify new S. cerevisiae Hsf1 protein interacting partners. Two GAL4 DNA binding domain-Hsf1 fusion proteins (baits) were constructed with mutations in the Hsf1 C-terminal activation domain to dampen Hsf1 mediated auto-activation of the …

Gradients In Season, Latitude, And Sea Ice: Their Effect On Metabolism And Stable Isotopic Composition Of Antarctic Micronekton, Erica H. Ombres

USF Tampa Graduate Theses and Dissertations

Respiration, metabolic enzyme assays, and body composition parameters were measured in the Antarctic krill Euphausia superba during the summer, fall and winter on the Western Antarctic Peninsula (WAP). E. superba of all sizes decrease their metabolism from the summer to the winter. These same parameters were also measured along the WAP during the austral fall 2010. E. superba's enzyme activity indicated that there was a latitudinal gradient to the decline in metabolism along the WAP with the more northerly sites having significantly higher metabolic enzyme activities than the sites to the south.

Carbon (δ¹³C) and nitrogen (δ^{15 …}

Functional And Mutational Analysis Of Human Recq-Like Dna Helicases In Saccharomyces Cerevisiae, Hamed Mirzaei-Souderjani

USF Tampa Graduate Theses and Dissertations

RecQ-like proteins are a family of DNA helicases that are evolutionary conserved from prokaryotes to eukaryotes. A large amount of experimental evidence suggests these proteins have a major role in the maintenance of genome stability. In humans five RecQ like helicase have been identified (RecQL1, BLM, WRN, RecQL4, and RecQL5), three of which are associated with rare genetic disorders with sever chromosomal and developmental abnormalities, and an elevated predisposition to cancer. Among the disease associated RecQ-like helicases, BLM and WRN have been subject to extensive research, while our collective knowledge about the function of RecQL4 is still very limited. Similarly, …

Light-Environment Controls And Basal Resource Use Of Planktonic And Benthic Primary Production, Kara Radabaugh

USF Tampa Graduate Theses and Dissertations

Consumers in marine and estuarine environments have a strong reliance on planktonic and benthic primary production. These two basal resources form the foundation of aquatic food webs, yet the abundance of phytoplankton and benthic algae are frequently inversely related due to competition for light and nutrients. As a result, optimal habitats for benthic and planktonic consumers vary spatially and temporally. To investigate these trends, three studies were conducted focusing on light attenuation and basal resources in a bay, river, and on a continental shelf.

δ¹³C and δ¹⁵N stable isotopes can be used as endogenous tracers to …

Sirt1 Regulation Of The Heat Shock Response In An Hsf1-Dependent Manner And The Impact Of Caloric Restriction, Rachel Rene Raynes

USF Tampa Graduate Theses and Dissertations

The heat shock response (HSR) is the cell's molecular reaction to protein damaging stress and is critical in the management of denatured proteins. Activation of HSF1, the master transcriptional regulator of the HSR, results in the induction of molecular chaperones called heat shock proteins (HSPs). Transcription of hsp genes is promoted by the hyperphosphorylation of HSF1, while the attenuation of the HSR is regulated by a dual mechanism involving negative feedback inhibition from HSPs and acetylation at a critical lysine residue within the DNA binding domain of HSF1, which results in a loss of affinity for DNA. SIRT1 is a …

Collecting Stardust: Matter, Memory, And Trauma In Patricio Guzman's Nostalgia For The Light, Nora Szegvari

USF Tampa Graduate Theses and Dissertations

This work situates Patricio Guzmàn's Nostalgia for the Light in the broader field of essay documentary film and unveils it as a locus of discursive resistance and the generative crux of diverse conventionally isolated academic dialogues. In doing so, it addresses the challenging and controversial questions of historical meaning-making, remembrance and oblivion, melancholia and mourning. My thesis also endeavors to detect the dynamic and anxiety-inducing threshold between singularity and collectivity, and the human and the cosmic. I lay the historically unprecedented common ground for trauma theory and the essayistic comportment and argue that bearing the clash of time planes, paradoxicality, …

Archaeological Site Distribution In The Apalachicola/Lower Chattahoochee River Valley Of Northwest Florida, Southwest Georgia, And Southeast Alabama, Adam M. Schieffer

USF Tampa Graduate Theses and Dissertations

This research examines and compares the distributions of archaeological sites and materials in order to investigate native settlement patterns and resources use throughout 12,000 years of prehistory and protohistoric time within the Apalachicola/Lower Chattahoochee River valley of northwest Florida, southwest Georgia, and southeast Alabama. Geographic Information Systems (GIS) are used to map the distributions of sites from different time periods and to explore their relation to various environmental characteristics that are now available in digital format. I employ tools now available in GIS to examine several longstanding research questions and expand upon archaeological interpretations within this region, where the University …

Refiguring Indexicality: Remediation, Film, & Memory In Contemporary Japanese Visual Media, Janine Marie Villot

USF Tampa Graduate Theses and Dissertations

Through an analog between film and memory, I argue contemporary Japanese visual media constantly remediates this relationship in order to develop a more inclusive, plastic indexicality that allows media without direct material contiguity access to an indexicality not typically attributed to it. Amidst the early twenty-first century shift from old, mechanical media to new, electronic media, each Japanese text engages the West through intercultural discourses and intracultural responses, just as Japan has continually encountered the West since its forced opening by Commodore Perry in 1853. The plasticized indexicality figured by contemporary Japanese visual media implies the plastic nature of abstracted …

Investigations For Utilizing Pteropods As Bioindicators Of Environmental Change Along The Western Antarctic Peninsula, Paul Mark Suprenand

USF Tampa Graduate Theses and Dissertations

Pteropods are holoplanktonic gastropod molluscs found globally. Although species diversity is greater at lower latitudes, species abundance is greater at temperate and polar latitudes. Declines in pteropod populations have not only been correlated to declines of their major predators, but pteropods have also been used as bioindicators of global environmental changes such as ocean acidification. With high latitude abundances, pteropods provide significant sustenance for species such as the Atlantic salmon in the Atlantic Ocean and Pleuragramma antarcticum in the Southern Ocean. Because pteropods eat phytoplankton and other pteropods, factors that affect pteropod abundance influence many trophic levels. This dissertation explores …

Affective Components Perceived To Be Important In Today's Global Society From A Cross-Cultural Perspective, Helena H. Wallenberg-Lerner

USF Tampa Graduate Theses and Dissertations

Global competencies, with differences in terminology by various researchers, had been frequently investigated, primarily from an American-biased perspective. Little or no defining research existed that identified requisite, universally agreed upon global competencies, or identified what affective components were perceived to be important cross culturally.

This research study answered the following questions:

1. What affective components are perceived to be important from a cross-cultural perspective?

2. Are there differences in these perceptions of affective components from a cross-cultural perspective?

The purpose of the study was to explore the extent to which individuals in different GeoCultural regions view and identify affective components …

Assessing Hurricane Preparedness Among Residential Staff At Louisiana State University: A Case Study On Hurricane Isaac, Ashley Marie Weatherall

USF Tampa Graduate Theses and Dissertations

Studying hurricane preparedness among the Resident Assistants (RAs) and Residence Life Coordinators (RLCs) at Louisiana State University (LSU) is imperative to assure that the university housing staff is fully equipped when faced with an oncoming threat. This study seeks to fill a gap in research by investigating the influences of preparedness on student housing employees. A survey was developed and the measures were found to be coherent and internally reliable through the use of factor analysis. Based on theory and previous literature, a linear regression model was developed that quantified the relationship between the independent variable of preparedness and general …

Eco-Epidemiology Of Eastern Equine Encephalitis Virus, Patrick Vander Kelen

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Eastern Equine Encephalitis virus (EEEV) is an alphavirus with high pathogenicity in both humans and horses. Florida continues to have the highest occurrence of human cases in the USA, with four fatalities recorded in 2010. Unlike other states, Florida supports year-round EEEV transmission. This research uses Geographic Information Science (GIS) to examine spatial patterns of documented sentinel seroconversions and horse cases in order to understand the relationships between habitat and transmission intensity of EEEV in Florida. Sentinel sites were categorized as enzootic, periodically enzootic, and negative based on the amount of chicken seroconversions to EEEV. Sentinel sites were analyzed …

The Effect Of Visual Search And Audio-Visual Entrainment On Episodic Memory, Holly Anne Westfall

USF Tampa Graduate Theses and Dissertations

Previous research suggests that larger context effects are observed when participants are required to search a scene in order to find the to-be-remembered stimuli. Similarly, animal research on brain oscillations has shown theta wave activation when animals are searching their environment. These theta wave oscillations are positively correlated with learning. However, theta activation can also occur in response to sensory stimulation, for example, auditory stimulation with binaural beats or visual stimulation with a checkerboard pattern reversal. The results of several studies suggest that while a visual search task seems to reliably improve free recall performance, the effects of passive sensory …

Phenotypic And Genotypic Analysis Of In Vitro Selected Miltefosine Resistant Leishmania Donovani, Brian A. Vesely

USF Tampa Graduate Theses and Dissertations

Abstract

Visceral leishmaniasis is a devastating neglected parasitic disease caused by infection with Leishmania donovani. It life cycle has two stages with promastigote (insect stage) and amastigote (animal stage) morphologies. Miltefosine is currently the only commercially available oral drug available to treat leishmaniasis and recent evidence suggests clinical resistance has emerged. Due to the importance of this drug and the scarcity of new drugs in the pipeline, work has been done on understanding the mechanism(s) of miltefosine, yet the mechanism of action for resistance is still not known.

In previous studies investigators generated miltefosine resistance on the insect vector stage …

"Of That Transfigured World" : Realism And Fantasy In Victorian Literature, Benjamin Jude Wright

USF Tampa Graduate Theses and Dissertations

"Of That Transfigured World" identifies a generally unremarked upon mode of nineteenth-century literature that intermingles realism and fantasy in order to address epistemological problems. I contend that works of Charles Dickens, Charlotte and Emily Brontë, Walter Pater, and Oscar Wilde maintain a realist core overlaid by fantastic elements that come from the language used to characterize the core narrative or from metatexts or paratexts (such as stories that characters tell). The fantastic in this way becomes a mode of interpretation in texts concerned with the problems of representation and the ability of literature to produce knowledge. Paradoxically, each of these …

Epigenetic Modifiers To Augment The Immunogenicity Of Chronic Lymphocytic Leukemia, Jason A. Dubovsky

USF Tampa Graduate Theses and Dissertations

Cancer cells employ a litany of immunosuppressive and immunevasive strategies to avoid detection and elimination by the various arms of the innate and adaptive immune system. Many hematologic and solid tumors progressively develop a specialized microenvironment which promotes tissue restructuring inflammation while masking the immune signature of the tumor cells themselves. Chronic lymphocytic leukemia, a malignancy of mature B lymphocytes must constantly balance on the precipice of immune recognition. A mature antigen presenting cell themselves, CLL clonal growth is dependent on the very interactions which, if effective, could potentially lead to their demise. To circumvent this, CLL clones set up …

Rhetorical Spirits: Spirituality As Rhetorical Device In New Age Womanist Of Color Texts, Ronisha Witlee Browdy

USF Tampa Graduate Theses and Dissertations

Throughout history African–American women have struggled against oppressions that have stereotyped their identities, scrutinized their character, and ultimately labeled their bodies inferior and inhuman. Despite the debilitating ideologies and barriers African–American women have been forced to operate within, they have fought against these racist, sexist, classist, homophobic environments, crafting their own “new” ethos through writing, as well as entertainment and popular culture. Although Black women remain plagued by history, the New Age of the 1980s as discussed by Akasha Gloria Hull in Soul Talk: The New Spirituality of African–American Women seemed to spark a new spirituality amongst African–American women. During …

Net Effects Of Batrachochytrium Dendrobatidis (Bd) And Fungicides On Anurans Across Life Stages, Jenise Brown

USF Tampa Graduate Theses and Dissertations

Amphibians are declining at alarming rates globally. Multiple factors contribute to these declines, including chemical contaminants and emergent diseases. In recent years, agrochemical use, especially fungicide applications, has increased considerably. Previous studies have demonstrated that these agrochemicals leave application sites and enter wetlands via runoff and have detrimental effects on non-target organisms. For example, exposure to contaminants can have multifarious effects on amphibians, such as reducing their ability to deal with a secondary stressor, such as disease.

A pathogen that is found concomitant with chemical contaminants in aquatic systems is Batrachochytrium dendrobatidis (Bd). Bd has decimated amphibian populations worldwide. Susceptibility …

Cost Of Treatment Of Asthma Attacks In A Tertiary Level Healthcare Hospital In Panama, Nydia Flores Chiari

USF Tampa Graduate Theses and Dissertations

Asthma is a chronic respiratory disease characterized by inflammation of the airway and the presence of recurrent attacks (exacerbations) of breathlessness, wheezing, cough, chest tightness, or some combination of these symptoms. In the US, about 53% of people with asthma had an asthma attack in 2008, and 57% of these, were children. One in ten children (10%) had asthma in 2009, and boys were more likely than girls to have asthma. Internationally, the prevalence of asthma varies widely in different countries, but the disparity is narrowing due to rising prevalence in low and middle income countries. Unfortunately, we do not …

The Water Culture Beliefs Of Embera Communities And Maternal And Child Health In The Republic Of Panama, Ilenia Anneth Forero

USF Tampa Graduate Theses and Dissertations

Water has cultural and spiritual values to indigenous people. These beliefs expose them to unsafe water sources and make them vulnerable to waterborne diseases. This background is not taken into account when countries write their water legislations, therefor imposing a management of water not readily accepted by them. The Embera group is one of the indigenous groups from the Republic of Panama, who have strong water beliefs. They live along the shore of rivers in houses built on high stilts away from urban areas. The purpose of this cross-sectional community based study is to describe through a survey the relation …

Relationship Between Dysphoric Moods, Risk-Taking Behaviors, And Toxoplasma Gondii Antibody Titers In Female Veterans, Allyson Radford Duffy

USF Tampa Graduate Theses and Dissertations

The number of female veterans is increasing daily. Previous research conducted on veterans has focused primarily on males or with small samples of females. Depression and suicidality are becoming increasingly evident in returning veterans. Toxoplasma gondii is an intracellular parasite that is common in the Middle East and has been reported to cause changes in personality and behavior.

The purpose of the current study was to examine relationships between T. gondii antibody titer and socioeconomic variables, dysphoric moods, and risk-taking behaviors in a sample of 70 female veterans. Blood samples were collected and analyzed for T. gondii antibody titer and …

African Americans And Hospice: A Culture-Centered Exploration Of Disparities In End-Of-Life Care, Patrick Dillon

USF Tampa Graduate Theses and Dissertations

As the United States' population ages and grows more diverse, scholars and practitioners have grown increasingly concerned about persistent disparities in the cost and quality of end-of-life health care, particularly with regard to African Americans. Although a variety of factors may influence these disparities, most scholars agree that the underutilization of hospice care by this population is an important contributor. Drawing from the culture-centered approach to health communication and narrative theory, the present study explores African American patients and caregivers' experiences with hospice care and takes an initial step toward addressing disparities in end-of-life care. I begin this study, first, …

The Translational Machinery As A Target For Radiosensitization, Thomas John Hayman

USF Tampa Graduate Theses and Dissertations

Current approaches aimed at improving the efficacy of radiation as a cancer treatment modality involve the development and application of molecularly targeted radiosensitizers, a strategy that requires a thorough understanding of the fundamental processes comprising the cellular radioresponse. Recent data indicating that radiation modifies gene expression primarily through translational control rather than transcriptional events suggests that mRNA translation contributes to cell survival after irradiation. The overall goal of this project is to determine whether the regulatory/rate-limiting components of the translational machinery provide targets for tumor cell radiosensitization. The majority of translation in mammalian cells occurs in a cap-dependent manner and …