Digital Commons Network^™

Targeting Histone Deacetylases In Melanoma And T-Cells To Improve Cancer Immunotherapy, Andressa Sodre De Castro Laino

USF Tampa Graduate Theses and Dissertations

Histone deacetylases (HDACs) are key mediators of gene expression and, thus, major regulators of cell function. As such, HDACs play a role in orchestrating tumor biology, and the use of small inhibitors targeting theses proteins is attractive for the field of cancer therapy. Indeed, several HDAC inhibitors have received FDA-approval for the treatment of malignancies, while a myriad of these compounds continue to be evaluated in clinical trials. Besides their direct impact on tumor growth, HDAC inhibitors have been shown to increase immunogenicity of cancer cells, facilitating generation of a productive immune response against tumors. Immunotherapeutic approaches take advantage of …

The Household Water Management System In The Village Of Falifah, Gambia: A Case Study In Sustainable Local Development, Baboucar Jobe

USF Tampa Graduate Theses and Dissertations

Access to safe and clean water is a problem in many countries in the world, especially sub-Saharan Africa. The urgency of the was recognized by the United Nations through its 2008 Millennium Development Goals, which were recently replaced by the new Sustainable Development Goals. Lack or poor access to clean water not only creates conflicts and rifts among the people, but also makes them more susceptible to a wide assortment of water borne diseases.

The purpose of this dissertation is to complete a pilot study of ways of thinking and actions of a small group of people from Falifah, a …

Chosen Champions: Medieval And Early Modern Heroes As Postcolonial Reactions To Tensions Between England And Europe, Jessica Trant Labossiere

USF Tampa Graduate Theses and Dissertations

This project explores connections between hero and history, text and context. By engaging Postcolonial theories about the roles that invasion and oppression, play in developing national identity and how colonized people respond to such encounters in literature, I examine how experiences of invasion and hostile interaction as represented in medieval and early modern English literature influenced the creation of specific heroic values.

In my first chapter, I analyze The Battle of Maldon and Beowulf as exemplars of the Anglo-Saxon culture, observing that Byrhtnoth and Beowulf work as fictional embodiments of a fantasy of power: men of super-human strength and exceptional …

Unraveling The Wild: A Cultural Logic Of Animal Stories In Contemporary Social Life, Damien Contessa

USF Tampa Graduate Theses and Dissertations

This dissertation is about the stories people tell about animals when they don’t do what they are expected to do in contemporary social life. More specifically, it examines three case studies where “wild” animals unexpectedly challenge, transgress, or blur socially defined boundaries in public spaces. Drawing on cultural and interactionist studies of animals and environment, I explore popular animal stories written in news media, social media, and enacted in situ. Each qualitative case study illustrates a moment in time/space where the surprising movements or presence of wild animals causes the cultural categories of wildness/order to breakdown and destabilize. These “surface …

The Construction Of Latino Im/Migrant Families In U.S. News Media: Parents’ Responses And Self-Representations, Jason Edward Miller

USF Tampa Graduate Theses and Dissertations

Latino im/migrants are often portrayed in negative and stereotypical ways in mainstream U.S. media. This dissertation utilizes Ethnographic Content Analysis to analyze news segments about Latino im/migrants from Fox News, MSNBC and Univisión between 2010 and 2012 and digital storytelling with a group of Latino im/migrant parents in central Florida.

First, I questioned if a Spanish-language news media source constructed Latino im/migrant family-focused stories differently than mainstream English-language sources. Utilizing Critical Race Theory as a theoretical lens, I conclude that English and Spanish-language news stations portray Latino im/migrants in different ways. Fox News portrays Latino im/migrants in a generally neutral …

Biophysical Investigation Of Amyloid Formation And Their Prion-Like Self-Replication, Mentor Mulaj

USF Tampa Graduate Theses and Dissertations

Growth and deposition of amyloid fibrils, polymers of proteins with a cross beta-sheet structure, are associated with a significant number of human pathologies including Alzheimer’s disease, Parkinson’s disease, prion diseases, type II diabetes, and senile systematic or dialysis-related amyloidoses. The broader objective of my research is to identify the basic mechanisms regulating nucleation and growth of amyloid fibrils. There is increasing evidence that amyloid formation may proceed along at least two distinct assembly pathways for the formation of rigid fibrils. One pathway involves the nucleated polymerization of the characteristic rigid fibrils from partially denatured monomers and the other proceeds via …

Novel Techniques In Chemical Ecology To Examine Life Histories In Fishes, Orian Tzadik

USF Tampa Graduate Theses and Dissertations

Diet and movements in fishes are often logistically challenging to study. Trace element and stable isotope analyses have advanced these fields considerably, but are still constrained by methodological impediments, such as the tendency towards lethal sampling. Studying endangered fishes is particularly challenging as representative samples are difficult to obtain. However, the information gained from such studies is often critical to the recovery of endangered fishes as knowledge of life history attributes has the potential to greatly influence the success of management strategies.

I tested the viability of using fin rays in fishes as a non-lethal approach to study diet and …

Acropora Habitat Evaluation And Restoration Site Selection Using A Species Distribution Modeling Approach, Katherine Wirt Ames

USF Tampa Graduate Theses and Dissertations

While populations of nearly all stony coral species along the Florida reef tract have exhibited decline, the most notable decline has occurred in the once-dominant acroporid species (Acropora cervicornis, A. palmata). Both species were listed in 2006 as threatened under the Endangered Species Act. This listing, combined with their continued decline, has resulted in large-scale restoration efforts throughout Florida and the Western Caribbean. Currently, there is little to no information regarding spatial prioritization of sites for these restoration efforts. The primary objective of this dissertation was to utilize species distribution modeling, informed by existing data from the …

Modeling And Survival Analysis Of Breast Cancer: A Statistical, Artificial Neural Network, And Decision Tree Approach, Venkateswara Rao Mudunuru

USF Tampa Graduate Theses and Dissertations

Survival analysis today is widely implemented in the fields of medical and biological sciences, social sciences, econometrics, and engineering. The basic principle behind the survival analysis implies to a statistical approach designed to take into account the amount of time utilized for a study period, or the study of time between entry into observation and a subsequent event. The event of interest pertains to death and the analysis consists of following the subject until death. Events or outcomes are defined by a transition from one discrete state to another at an instantaneous moment in time. In the recent years, research …

Examining The Impact Of Deictic Relational Responding On Advanced Theory Of Mind And Pretense In Children With Autism, Samantha Lee Broderick

USF Tampa Graduate Theses and Dissertations

Perspective taking is a pivotal behavioral repertoire essential for social functioning and is recognized as a hallmark deficit of the Autism Spectrum Disorder. Recent advancements in the Relational Frame Theory have led to the development of a perspective-taking training protocol shown to improve performance on Theory of Mind tasks in typically developing children; however, there has been little research on the generality of these findings in children with ASD. The impact of deictic responding on social interaction is also undetermined. The current study aimed to: a), evaluate the effectiveness of multiple exemplar training of deictic relations on perspective taking abilities …

Disinhibition, Violence Exposure, And Delinquency: A Test Of How Self-Control Affects The Impact Of Exposure To Violence, Wyatt Brown

USF Tampa Graduate Theses and Dissertations

This dissertation examines the role of self-control in the relationship between exposure to violence and antisocial behavior. Specifically, this study proposes that the impact of exposure to violence changes depending on internalized factors such as self-control. Individuals with high exposure to violence but greater levels of self-control may be less influenced by the impact of exposure to violence. Conversely, individuals with low levels of exposure to violence and lower levels of self-control may be more influenced by the impact of violence. The findings from this study suggest that there is some buffering effect on the impact of exposure to violence …

Sizing An Anaerobic Digester In A Rural Developing World Community: Does Household Fuel Demand Match Greenhouse Gas Production?, Ronald Keelan Greenwade

USF Tampa Graduate Theses and Dissertations

Anaerobic digestion is the process by which organic carbon is converted into biogas in the form of carbon dioxide (CO₂) and methane (CH₄). Both of these products are greenhouse gases that contribute to global warming. Therefore if anaerobic reactors are improperly maintained and biogas is leaked or intentionally released into the atmosphere because biogas production exceeds household demand, these reactors may become generators of greenhouse gas emissions instead of sustainable energy producers. The objective of this research was to develop a framework to assess if the demand for biogas by a rural adopter of an anaerobic …

Application Of Spatial And Descriptive Analysis Methods To Determine Relationship Between Hardware Subsidies And The Sanitation Marketplace, Danielle Devuyst

USF Tampa Graduate Theses and Dissertations

Sanitation marketing is an emerging approach of strengthening the local private sector to implement scalable and sustainable improved sanitation coverage in developing countries, specifically among the poor. It encourages the enhancement of sanitation market supply and demand by developing distribution infrastructure and stimulating consumer interest. Unlike interventions that provide hardware subsidies to initiate sanitation demand, financial support for sanitation marketing is used exclusively for the research and development of the market; this encourages the private sector to become independent and self-sufficient. Qualitative data suggests that while sanitation marketing projects have been successful at implementing replicable and sustainable sanitation coverage, they …

Adoption Of The Sawyer Water Filter In Peru, Stephanie D. Paredes

USF Tampa Graduate Theses and Dissertations

In Peru, lack of water, sanitation, and hygiene (WASH) results in 6,600 deaths per year that represents approximately 3.9% of total deaths. Three thousand and nine hundred of these deaths were due to diarrheal diseases (Prüss-Üstün et al., 2008). Systematic reviews suggest that interventions to improve microbial quality of drinking water are successful in reducing diarrheal diseases (Fewtrell et al., 2005; Clasen et al., 2007; Fry et al., 2013). Interventions for household water treatment and safe storage to ensure safe drinking water reduce diarrhea by 31-52% (WHO/UNICEF, 2013).

The SAWYER PointONE filter, a portable and adaptable membrane filtration device as …

The Impact Of A Teacher-Preferred Group Contingency With Data-Based Decision Making On Class-Wide Behavior, Fernando M. Herrera

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to further examine the impact of a teacher-preferred group contingency on class-wide behavior in three general elementary education classrooms when it is used with data-based decision making by classroom teachers. A multiple baseline design across classrooms was used to examine the changes in class-wide disruptive behavior, academic engagement, and academic performance in targeted academic time periods. Data indicated that implementation of the group contingency preferred by the teachers in conjunction with data-based decision making resulted in decreases in disruptive behavior and increases in academic engagement and academic performance across classrooms. In addition, improvement in …

Tough Guy, Sensitive Vas: Analyzing Masculinity, Male Contraceptives & The Sexual Division Of Labor, Kaeleen Kosmo

USF Tampa Graduate Theses and Dissertations

A Marxist feminist standpoint positions patriarchy and capitalism as mutually beneficial, thus interestingly situating the new market of male contraceptives (MCs). This project takes an in-depth look at the opinions of 15 young men regarding the use of MCs by examining how Western, heterosexual masculinity informs their attitudes and discusses how a new economic market of MCs may affect current social ideologies about of the sexual division of labor. Because notions of masculinity are essential in perpetuating such ideologies, understanding masculinity as it relates to a new market for MCs is imperative. During a series of focus groups men described …

Selling The American Body: The Construction Of American Identity Through The Slave Trade, Max W. Plumpton

USF Tampa Graduate Theses and Dissertations

In this thesis I argue that the early conceptualization of American identity was achieved through the dehumanization of blacks at slave auctions, and that the subjugation of this group informed more areas of the collective, normalized, American identity than just race. I contend that blacks were deprived of qualities that are considered inherently human (and American) and reduced to the facts of their bodies. To do this, I analyze newspaper advertisements for slave auctions, abolitionist editorials, and postings for runaway slaves. I also look at primary accounts of slave auctions that speak to the performative nature of the setting. I …

Conversion Of Landfill Gas To Liquid Hydrocarbon Fuels: Design And Feasibility Study, Ryan Alexander Kent

USF Tampa Graduate Theses and Dissertations

This paper will discuss the conversion of gas produced from biomass into liquid fuel through the combination of naturally occurring processes, which occur in landfills and anaerobic digesters, and a gas-to-liquids (GTL) facility. Landfills and anaerobic digesters produce gases (LFG) that can be converted into syngas via a Tri-reforming process and then synthesized into man-made hydrocarbon mixtures using Fischer-Tropsch synthesis. Further processing allows for the separation into liquid hydrocarbon fuels such as diesel and gasoline, as well as other middle distillate fuels. Conversion of landfill gas into liquid fuels increases their energy density, ease of storage, and open market potential …

A Study On The Effect Of Non – Asthmatic Atopy On Pregnancy Outcomes, Ayesha Johnson

USF Tampa Graduate Theses and Dissertations

Objectives – To measure the effect of non – asthmatic atopy (NAA) on the risk of adverse pregnancy outcomes; low birth weight (LBW), small for gestational age (SGA) and preterm birth (PTB), among primiparous women 19 – 25 years of age.

Methods – Vital Statistics records from births occurring between 2004 and 2014, from approximately 60,000 primiparous women in South Carolina, aged 19 to 25 years, were linked to their Medicaid records. Maternal records were examined to determine if they had a previous diagnosis of NAA. Women with a diagnosis of NAA were frequency matched with a ratio of 1:4 …

Effects Of Delay To Reinforcement On Selections For High-Tech And Low-Tech Leisure Items, Yuram Kim

USF Tampa Graduate Theses and Dissertations

Many children are exposed to excessively technology. Such use of technology may lead to health issues including obesity, attention deficits, and sleep disorders. Research has shown that parameters of reinforcement, such as quality and delay, may influence how children allocate their preferences. One way to drive preference away from high-tech toys may be to arrange delays to reinforcement following such selections and immediate reinforcement for an alternative response. In Experiment 1, four subjects who preferred high-tech leisure items over low-tech leisure items were identified through the pair-stimulus preference assessments. The results of Experiment 2 indicated that all subjects were sensitive …

To Utopianize The Mundane: Sound And Image In Country Musicals, Siyuan Ma

USF Tampa Graduate Theses and Dissertations

Many consider music, songs, and dance performance as utopian signifiers for cinema, but few has entered the utopian discourse of country musicals, a small genre of cinema usually known as country music films. By closely scrutinizing Pure Country (1992), this thesis aims to reveal how country music—as music numbers and as background cues— integrate and connect the fragmented on-screen world for the country musicals so as to offer audiences a fullness of utopian experience, and how this utopian effect are culturally significant for American audiences due to country music’s unique mechanism of constructing utopia and nostalgia in its past-orientations, sentimentalities, …

Reinforcement Of Variability And Implications For Creativity, Harvey Ray Bayliss

USF Tampa Graduate Theses and Dissertations

One of the defining characteristics of Autism Spectrum Disorder (ASD) is repetitive, rigid, or stereotyped patterns of behavior. A proposed approach to treating such patterns is to provide reinforcement for response variability. Though research demonstrates that the variability of responses can be influenced by contingencies of reinforcement, no studies have examined the effects of placing contingencies on different units of behavior. The purpose of this study was to examine effects of two modified percentile schedules on variety of completed drawings and individual lines drawn by students with ASD who had been referred for engaging in rigid patterns of behavior. For …

Assessment Of The Role Of Poly (Adp-Ribose) Polymerase In Drug-Induced Cardiomyopathy, Alexis I. Brinkerhoff

USF Tampa Graduate Theses and Dissertations

Drug-induced cardiotoxicity has resulted in a thorough evaluation of patient doses, treatments, and rehabilitation. One of the most commonly prescribed chemotherapeutic agents is cyclophosphamide. The active metabolite, acrolein, is one of the most potent inducers of cardiomyopathy. In this study, research was conducted on the H9c2 (2-1) cardiomyocyte cell line derived from the embryonic myocardium of rattus norvegicus to assess its competency for evaluation of the change in poly (ADP-ribose) polymerase (PARP) activity. The application of this model to study the effects of acrolein on PARP activation was chosen as an ideal determinant of cell damage produced by nitrogen mustards. …

Utilizing Immediate Feedback In Piano Pedagogy, Michael Szabo

USF Tampa Graduate Theses and Dissertations

Piano pedagogy is the study of the teaching of piano performance. Several effective methods have been developed since the early 1700's, but lack empirically supported techniques. Immediate feedback procedures have been shown to be effective with skill acquisition in various capacities within the literature. While some innovative techniques are being developed which utilize technologies such as video and sensor-based feedback, the true impact of these interventions has not been empirically validated. There is also a paucity of research in the behavioral literature evaluating the efficacy of immediate feedback procedures in acquisition of music performance. The current study evaluated the effectiveness …

Concerns Of Water Scarcity And Water Quality Among Two Andean Communities In Peru, Kelsey Anne Anderson

USF Tampa Graduate Theses and Dissertations

This thesis sought to explore the perceptions and experiences of Andean women regarding water quality, water scarcity, and health among two communities in Carhuaz province of Ancash, Peru. Household surveys (n=25), semi-structured interviews (n=10), unstructured interviews (n=2), and participant observation were conducted with local women to investigate their concerns and perceptions of water and health. An additional two unstructured interviews were conducted with a local water authority and doctor in order gain another perspective on the issues of water security and health.

The pressure of a changing climate and of a problematic water governance system in Andean Peru create an …

"But Our Hands Are Tied": Assessing School Gardening Efforts At Title I Elementary Schools In Pinellas County, Florida, Alexandra Grace Lancey

USF Tampa Graduate Theses and Dissertations

This research was designed to understand current school gardening efforts in Pinellas County, Florida. School gardens have become an important aspect of experiential learning and nutrition education in schools throughout the United States. Many not-for-profit organizations have attempted to increase the prevalence and efficacy of school garden programs as a means of providing educational opportunities and working to curb diet-related health issues in children. Most of these organizations are seen as apolitical in nature, because they access mainly private sector funding sources and volunteer support. This provides flexibility for these social projects, but also takes pressure off of the state …

Constructing The West: The Hired Hand And Mccabe & Mrs. Miller And The Challenge Of Public Space, Eric Ward Ross

USF Tampa Graduate Theses and Dissertations

The Western has been an important and iconic part of American culture since the opening of the frontier. However, very few scholars have looked closely at the way the genre constructs the past through public and private spaces like frontier towns and settlements.

The 1971 films, The Hired Hand and McCabe & Mrs. Miller are two texts that revitalized, and in the process revised, the Western genre in the early 1970s. My paper examines the ways in which conflicts between public and private spaces in the films reflect the social and cultural conflicts in America at the time. Both films …

Multiple Case Study Of Factors Associated With Enrollment Of Women With Persistent Or Recurrent Ovarian Cancer In Clinical Trials In Central Florida, Judith Ann Ebbert

USF Tampa Graduate Theses and Dissertations

Study Purpose: Ovarian cancer, the most lethal gynecologic cancer, has had a relatively stable mortality rate since 1975, despite a decrease in mortality for all gynecologic cancers combined. Standard-of-care advances are needed to reduce ovarian cancer morbidity and mortality. Advances must, however, undergo a long, rigorously controlled research process that can take more than ten years before becoming available to the public. Further, few women with persistent or recurrent ovarian cancer are offered or enrolled in the clinical trials that do exist at select sites throughout the nation. The purpose of this multiple-case study is to identify necessary and/or sufficient …

Characterization Of Bacterial Diversity In Cold-Water Anthothelidae Corals, Stephanie Nichole Lawler

USF Tampa Graduate Theses and Dissertations

Cold-water corals, similar to tropical corals, contain a diverse and complex microbial landscape. Comprised of vital microscopic organisms (i.e. bacteria, viruses, archaea), the coral microbiome is a driving factor in the proliferation and survival of the coral host. Bacteria provide essential biological functions within coral holobionts, facilitating increased nutrient utilization and production of antimicrobial compounds. To date, few cold-water octocoral species have been analyzed to explore the diversity and abundance of their microbial associates. For this study, 23 samples of the family Anthothelidae were collected from Norfolk (n = 12) and Baltimore Canyons (n = 11) from the western Atlantic …

Regulatory Effect Of Elastin Based Biomaterial On Cellular Behavior And Its Application On Wound Repair And Regeneration, Yuan Yuan

USF Tampa Graduate Theses and Dissertations

Elastin-like peptides (ELPs) are stimulus-responsive protein-based polymers which are attractive material for biomedical research due to their biocompatibility and unique properties. The physical properties of ELPs are dependent on the chain length and the chosen amino acid at the guest residue position. This imparts unlimited flexibility in designing ELP based biomaterials with the desired physical properties.

We have shown that in addition to their physical properties, ELPs have biological activities that are conducive to tissue regeneration. Specifically, we found that ELPs induce fibroblast proliferation via cell surface heparan sulfate proteoglycans (HSPG). Furthermore, our data suggests that ELP based materials with …