The Cultural Diplomacy Of U.S. Peace Corps: Understanding The Role Of Peace Corps Volunteers As Strategic Geopolitical Assets, Corey B. Miles

Theses and Dissertations

Beginning in the Summer of 1961, the United States government has deployed volunteer humanitarians around the world to serve the mission of U.S. Peace Corps and has since contributed to communities in 143 partner countries while enjoying widespread popularity. Its mission of promoting world peace and friendship is pursued through three operational goals: technical training, cultural exportation, and cultural importation. Since 2016, with the election of U.S. President Donald Trump, and continuing through the Covid-19 pandemic, which produced the first global service interruption in agency history, the value of the Peace Corps service model has been fundamentally challenged. As a …

Well-Being From Home To Work: The Role Of Mindfulness, Alisha Gupta

Theses and Dissertations

Considering the growing spillover of work and home and its impact on well-being, I draw from the Work-Home Resources Model and Attention Restoration Theory to examine the effects of evening recovery experiences at home on mindfulness at work and eudaimonic workplace well-being. Specifically, I focus on two indicators of eudaimonic well-being—one indicator of psychological functioning (work meaningfulness) and one indicator of social functioning (high-quality connections; HQCs). Further, I introduce a boundary condition, distance from work, of the relationship between evening recovery experiences and mindfulness. I suggest that due to alternative work arrangements in today’s workforce (i.e., that permit increased overlap …

Mitigation Of Nicotine Reward And Withdrawal By The N-Acyl Amino Acids: N-Oleoyl Glycine And N-Oleoyl Alanine, Kimberly Karin

Theses and Dissertations

A growing body of research is exploring the efficacy of N-acyl amino acids (N-oleoyl glycine; OlGly and N-oleoyl alanine; OlAla) in rodent models of drug reward and dependence. In particular, OlGly attenuated the reinforcing effects of nicotine in the mouse nicotine-induced conditioned place preference (CPP) paradigm and reduced somatic and affective signs of mecamylamine-precipitated withdrawal in nicotine-dependent mice. Based on the addition of a methyl group on the head group, OlAla was proposed to have increased metabolic stability compared to OlGly. While OlAla has been assessed in assays of reward and withdrawal of opioids, cocaine, and …

Anti-Immigration-Related Stressors And Latine Immigrant Well-Being: The Role Of Family & Community Resilience, Lisa S. Fuentes

Theses and Dissertations

Anti-immigrant rhetoric and policies have continued to adversely impact Deferred Action for Childhood Arrivals (DACA) and Temporary Protection Status (TPS) beneficiaries (Arevalo et al., 2015; Cleary, 2017; Li, 2015; Sangalang et al., 2018). There have been numerous attempts to revoke DACA, TPS, and other policies that temporarily protect immigrant families. Although the number of resilience literature has grown, more strength-based approaches are still needed. Additionally, given that familismo (familism) and collectivism are key cultural values for Latine families (Campos et al., 2019; Corona et al., 2017), it is imperative to explore family and community resilience among Latine immigrants to gain …

Initial Psychometric Evaluation Of An Observer-Rated Measure Of Shared Decision Making Practices In Youth Psychotherapy, Jennifer Lc Herbst

Theses and Dissertations

SDM is a process by which clinicians and clients collaboratively make treatment decisions; this process may improve the alliance and clinical outcomes. SDM in youth psychotherapy is understudied, and a critical gap in the field is the paucity of established measures designed to assess SDM practices in youth psychotherapy. This project aimed to fill this gap by examining the score reliability and validity of a novel observer-rated measure of SDM designed for youth psychotherapy—the Shared Decision Making Practices in Youth Mental Health scale (SDM-YMH)—and using the measure to investigate linkages between SDM practices, the alliance, and clinical outcomes. Therapy tapes …

Quantitative And Molecular Genetic Analyses Of Brain Structure, Substance Use, And Posttraumatic Stress Phenotypes: Data From Children, Adolescents And Adults, Daniel Bustamante

Theses and Dissertations

Abstract

QUANTITATIVE AND MOLECULAR GENETIC ANALYSES OF BRAIN-IMAGING, SUBSTANCE USE, AND POSTTRAUMATIC STRESS PHENOTYPES: DATA FROM CHILDREN, ADOLESCENTS AND ADULTS

By Daniel Bustamante, B.S., B.A.

Doctoral dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Integrative Life Sciences – Behavioral and Statistical Genetics at Virginia Commonwealth University.

Virginia Commonwealth University, 2023.

Dissertation directors: Michael C. Neale, Ph.D., Banks Distinguished Professor of Psychiatry & Human and Molecular Genetics; and, Ananda B. Amstadter, Ph.D., Professor of Psychiatry, Psychology, & Human and Molecular Genetics.

Substance use (SU) and posttraumatic stress disorder (PTSD) phenotypes are comorbid, share …

Vulnerability And Adaptation Of Neocortical Neuron Subtypes In Models Of Concussive Traumatic Brain Injury And Multiple Sclerosis, Alan C. Harris Jr.

Theses and Dissertations

The pathognomonic sequela of traumatic brain injury is traumatic axonal injury, the

burden of which correlates with less favorable outcomes. Elucidation of

the intrinsic variables that endow delineated neuronal subgroups and microanatomical domains with outsized vulnerability to injury represents a critical domain of inquiry toward the successful translation of disease-modifying therapeutics. To this end, we studied mild traumatic brain injury (mTBI) within the central fluid percussion model to examine the consequences of mTBI on the morphology and functional integrity of the axon initial segment (AIS) and the relative contributions to network homeostasis mediated by distinct populations of inhibitory interneurons. One …

Investigation Of Sneathia Vaginalis And The Host Antibody Response To S. Vaginalis Pore-Forming Toxin, Cpta, In Vivo, Zion T. Mccoy

Theses and Dissertations

Sneathia vaginalis, formerly Sneathia amnii, is a gram-negative vaginal species that is associated with pregnancy complications including preterm birth. The only virulence factor of S. vaginalis that has been characterized is the cytopathogenic toxin, component A (CptA), a pore-forming toxin that lyses human red blood cells and human epithelial cells and plays a role in traversal of the bacteria through fetal membranes. The overarching goal of this work was to examine the host immune response to CptA. To investigate the immune response to CptA, levels of anti-CptA IgM, IgA, and IgG in human mid-vaginal swab samples obtained during …

A Theory Of General Relativity, Madeleine Mae Morris

Theses and Dissertations

"A Theory of General Relativity" is a quiet exploration into the poetic and scientific ways we inhabit space and time. This work is a manifestation of the time I spent being my grandmother's caretaker and exists now in the form of an artist book, an exhibition of framed photographs with glass objects, and this written thesis. Throughout those five years, I became consumed by the relationships between Mothers and Daughters, which brought into excruciatingly sharp focus seemingly simple questions like: who are we, what have we done to each other, what do we owe to each other, do I reap …

Totem Impulse, Tendai T. Mupita

Theses and Dissertations

In this reading, I explore the performative and complexities of indigenous mythologies, cosmologies, and epistemologies, through totems, folklore, architecture, and games. This paper extracts references from indigenous philosophies in thinking about form and image. These forms and images are my sculptures, my installations, and my lines.

Treponema Denticola Synthesizes C-Di-Amp And Encodes The Cdaa-Type Diadenylate Cyclase Cdaa, Claire R. O'Brien

Theses and Dissertations

Periodontitis is a form of oral disease characterized by dysbiosis of the oral microbiome, leading to inflammation, bone resorption, and in severe cases, entire tooth loss, affecting 42% of adults in the US. One of the bacteria most associated with periodontal disease progression is Treponema denticola (Td), an oral spirochete which inhabits the mouth in small quantities during health but which can dominate the biofilms that form during periodontal disease. The ability of Td to survive in a disease environment and contribute to the progression of disease requires the use of robust signaling networks. Analysis of Td cultures …

Characterization And Investigation Of Cold Atmospheric Plasma And Its Effects On Cancer Cell Biology, Thomas M. Ritrosky

Theses and Dissertations

Modern cancer treatment uses radiation therapy in over 50% of patient cases. It is an e↵ective way of treating tumors because the mechanisms of cell killing are well known through the damage that ionizing radiation does to DNA. The amount of radiation can be tracked through measuring the dose of the clinical photon or electron beam used. However, there are limitations in the usage of radiation therapy, for example, a tumor can create hypoxic areas that become radioresistant leading to complete ine↵ectiveness of further radiation treatment. This project looks into the application of cold atmospheric plasma as an adjuvant therapy …

Sense Of Belonging As Behaviorist Construct: “Doing Belonging” And Its Application For College Students With Disabilities, Chris Parthemos Phd

Theses and Dissertations

Sense of Belonging has been widely linked to academic performance, retention and persistence for college students. Students with disabilities are less likely to enroll than peers, and are more likely to drop out. Similar to other historically-marginalized groups, these students report lower sense of belonging than peers. Research with this population suggests that they may be seeking ways of building community and belonging at their universities which are self-driven. This notion of “doing belonging”, as it is described in the sociological literature, appears at first glance to be compatible with behaviorist approaches to student support. Although not without controversy, behavior …

Tgif1 Physiological Levels Limit Β-Cell Distress And Neonatal Diabetes, Creighton J. Friend

Theses and Dissertations

TGIF1 belongs to the superfamily of homeodomain proteins, which regulate a wide variety of biological functions, including cell stemness and specification of cell fate during early development. Perhaps surprisingly, we found that enforced expression in pancreatic progenitor cells during embryogenesis resulted in severe diabetes, hinting at the possibility that TGIF1 might regulate pancreas development. Subsequent genetic experiments targeting β-cells showed that TGIF1 affected β-cell function and homeostasis. Transcriptomic analysis revealed that TGIF1 expression inhibits the expression of essential components of UPR signaling, underscoring a potential mechanism in which TGIF1 disrupts protein folding and secretion. Congruently, TGIF1 expression led to a …

Buckets In A Deep Well: Essays About Shakespeare’S History Play Characters And An Original Drama, Brian C. Bassett

Theses and Dissertations

Buckets in a Deep Well is a set of essays that examines various characters portrayed in Shakespeare’s history plays, as well as parallel figures in 20th and 21st century works. Each essay primarily focuses on a single character’s response to a power structure: Richard Gloucester to the Tudor England of his audience in Richard III; Richard Nixon’s campaign-produced version of himself to 20th century American democracy in “Checkers;” Falstaff to organized society in Henry IV, Part 1; Marlo Stanfield to the drug economy of early 2000’s Baltimore in The Wire; the Duke of York to the disrupted monarchy of The …

Effectiveness Of Financial Incentives In Improving Breast Cancer Screening Among Medicaid Recipients, James B. Sherwood

Theses and Dissertations

Breast cancer remains one of the leading causes of death among women. Roughly 2.4 million women are diagnosed annually with breast cancer throughout the world. Although breast cancer survival rates are favorable for the United States compared to other nations (90% 5-year survival for non-metastatic disease), it comes at a high cost. The United States spends an estimated $30 billion annually on breast cancer treatment. In addition, disparities in breast cancer survival rates also exist in the United States. Women Medicaid recipients, who are predominately minorities (60%), are more likely to die of breast cancer due to their high rates …

Formation Of Vasculogenic Mimicry: Role Of Melanoma Differentiation Associated Gene-9 In Human Melanoma, Jinkal Shah

Theses and Dissertations

Malignant melanoma (MM) is the most aggressive skin cancer and the most frequent skin disorder in Caucasians. MM is associated with aggressive and progressive disease states, leading to major cancer-related morbidity and mortality. Recent investigations identify an alternative pathway -vasculogenic mimicry (VM), which is considered a cancer hallmark that can independently facilitate tumor neovascularization by the formation of fluid-conducting and vascular endothelial cells. MM cells undergoing VM can dedifferentiate into numerous cellular phenotypes and acquire endothelial-like features, resulting in the formation of the de novo matrix-rich vascular-like networks, such as plasma and red blood cells. The co-generation of endothelial cells, …

Wrestling With The One Drop Rule: Exploring Multiraciality In Social Work Education, Britney Pitts

Theses and Dissertations

This three paper dissertation aims to provide a baseline understanding of how multiraciality is understood in social work education. The first paper, a fusion autoethnography, explores how my own perception of my multiracial identity was clarified throughout my social work education. This exploration led to the development of incongruent logic in/validation (ILV), a new aspect of racial identity invalidation (RII). The second paper, a multimethod qualitative case study, explores master of social work (MSW) students’ knowledge, attitudes, and anticipated professional behaviors with multiracial individuals and families. Themes from this study include: never explicitly learned about multiraciality; non-ecological endorsements of multiracial …

Collaborative Lesson Study: A Qualitative Case Study Of Elementary Teachers’ Professional Learning Experience, Arlene D. Harmon

Theses and Dissertations

Teacher collaboration practices in schools have impacted how teachers work together to prevent professional isolation. The mandate of collaborative practices has resulted in some teachers expressing frustration and a lack of benefit from their current experiences. The purpose of this qualitative case study, conducted in the Southeast region of the United States, was to determine if lesson study provided a more meaningful collaborative approach during focused group sessions. Using New London Group’s (1996) designs of meaning concept encapsulated in the Pedagogy of Multiliteracies Theoretical Framework to gauge the relevance of lesson study, I sought to explore a deeper understanding of …

The Effect Of Social Stratification On College Students' Academic Performance & Progress, Detrenyona Nekebra Chester

Theses and Dissertations

The purpose of this quantitative correlational research study is to understand the variables of social stratification, academic performance, and academic progress and how those variables affect a student’s decision to drop out of college. The researcher selected participants for this study from the database at a 4-year private HBCU located in the northern part of Jacksonville Florida. Many students enrolled in college encounter outstanding tuition balances that threaten their ability to obtain adequate housing and remain actively enrolled in college. The researcher explored social stratification as measured by high school Title I status, academic performance as measured by overall institutional …

Adapting Higher Education: Revamping Curricula For The Inclusion Of Theatre Students With Disabilities, Kevin Kemler

Theses and Dissertations

Equity, Diversity, and Inclusion initiatives in higher education have been largely driven by administrators who have little to no contact with the students for whom they are working for. This top-down approach negatively impacts marginalized students and disproportionately affects the quality of experience for students with Disabilities, an often-overlooked demographic. For Disabled students enrolled in performance programs, barriers to access and inclusion don’t just exist at the institutional level, they also exist in the traditional classroom or studio as well. Through a dismantling of ableist structures inherent within higher education (i.e., American grading practices, the Western and Theatrical Canons), I …

Contribution Of Metabolic Cell Swelling On Microcirculatory Perfusion In Septic Shock, Charles E. Payne

Theses and Dissertations

Sepsis, a systemic immunological response that leads to organ-dysfunction, the development of septic shock, and death has mortality rate of 15-25%. A subset of sepsis, septic shock, has an in-hospital mortality rate of 30-50%. Sepsis and its treatment are the most expensive healthcare problem in the United States with the US Agency for Healthcare Research and Quality estimating annual costs over $20 billion. Hypoxia experienced in septic shock induces metabolic cell and tissue swelling leading to further microcirculatory dysfunction and poor tissue perfusion. Reversing metabolic cell swelling corrects these perfusion defects. Two models were used in rats to induce septic …

Children With Special Healthcare Needs: The Cost Of Medical Care, Kasey Dye

Theses and Dissertations

The purpose of this study was to explore factors that impact children with special healthcare needs’ (CSHCN) health insurance coverage and out-of-pocket medical expenses. The Ecological Model for Health Promotion developed by McLeroy et al. (1988) was the guiding theoretical framework for this study. Using data from the 2020 National Survey of Children’s Health, I conducted descriptive statistics, logistic regressions, and multilevel modeling analyses to determine if health insurance coverage and out-of-pocket expenses for CSHCN differed by individual, family, and community, organization, and policy factors. Results indicated that race, gender, disorder category, insurance type, and family’s federal poverty level were …

The Use Of Silver Diamine Fluoride In Dentistry In Virginia, Jessica Eisenberg

Theses and Dissertations

Purpose: The purpose of this research was to determine the use of silver diamine fluoride (SDF) in dentistry in Virginia and the factors that affect the practitioners’ decision. Methods: A survey was sent to the members of the Virginia Dental Association (VDA) by email and posted on the VDA social media accounts. The survey questions measured the practitioner’s demographics, knowledge, and factors that affect their utilization of SDF. Results: Most providers were general dentists (77%). The most common clinical situations for SDF use were to arrest caries in children with behavioral issues (77%), to delay restorative treatment (73%), in medically …

Provider Racial Concordance And Medical Mistrust In Pediatric Dentistry, Chinelo Eke

Theses and Dissertations

Purpose: Studies have drawn varying conclusions on the importance of a racially concordant patient provider relationship. Medical mistrust has continued to garner attention as healthcare enterprises thread equity, diversity and inclusion throughout their organizational policies, practices and systems.

Methods: Parents/caregivers of pediatric patients at VCU Pediatric Dentistry were invited to participate. Surveys included questions about demographics, racial concordance, language preference, and the validated Group-Based Medical Mistrust Scale.

Results: A total of 246 guardians completed the survey. For guardians, 40% reported Black or African American, 31% Hispanic, and 19% Caucasian. Seventy-one percent of guardians reported speaking English, 18% Spanish, and 11% …

The Age And Primary Reason For The First Dental Visit In Children With Special Health Care Needs, Amanda Dickerson

Theses and Dissertations

Purpose: The aim of this project is to evaluate the average age and primary reason for the first dental visit among patients with special health care needs as well as to examine socioeconomic factors and other barriers related to children with special health care needs accessing care.

Methods: This is a cross-sectional survey that was distributed to caregivers of children with special health care needs. Study data was collected through REDCap, a secure web-based survey application. The inclusion criteria for participating in the survey included having a child with a special health care need that presented for a recall or …

Utilization Of Intraoral Scanners For Fabrication Of Obturators For Patients Born With Cleft Lip/ Palate (Clp), Swara Fadnis

Theses and Dissertations

Objective: The goal of this cross-sectional study was to assess the knowledge and attitude of providers regarding incorporating a digital scanner for impression taking in children born with cleft lip and/or palate (CLP). Methods: A survey was sent out to members of the American Association of Pediatric Dentistry (AAPD). The survey included questions regarding the providers’ perception of incorporating digital intraoral scanners for impressions for fabrication of obturators. The survey also included questions about the providers’ reservations in using this technology in practice, what their experience has been with the incorporation of this technology into practice, and what is …

Home Disruption And Substance Use: The Moderated-Mediating Role Of Family Conflict And Stress Among Racial-Ethnic Minoritized College Students, Geovani Muñoz

Theses and Dissertations

The COVID-19 pandemic has disproportionately impacted first-generation college students who identify as racial-ethnic minorities (REM). Aside from the academic and health repercussions caused by COVID-19, little is known if home disruptions have increased substance use for REM college students. Specifically, increased home disruptions may directly relate to substance use and indirect relationships through increased family conflict and stress. Therefore, this master's thesis examined the direct relationships between home disruption and substance use. I also examined the relationships between home disruption and family conflict, as well as home disruption and stress. Further, I also investigated the relationships between family conflict and …

Histone Acetylation In Periodontal Diseases, Sarang Saadat

Theses and Dissertations

Background: Emerging data has indicated the significant role of epigenetic control in inflammation. Acetylation of lysine residues on histones is a major epigenetic modification of chromatin, which is required for cytokine gene expression and regulated in inflammatory diseases. It has been shown that periodontal bacteria induce histone acetylation in oral epithelial cells and experimental periodontitis in rodents. In this study, we investigated the acetylation level of histone 3 (H3) in gingival tissue from healthy and periodontitis patients. We hypothesize that cytokine over-production in periodontitis is associated with the hyper acetylation of histones. Methods: Periodontitis and healthy subjects who need periodontal …

The Wound Healing And Antibacterial Properties Of Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles, Emily N. Wandling

Theses and Dissertations

Treatments for acute respiratory distress syndrome (ARDS) are still unavailable and the prevalence of the disease has only increased due to the Covid-19 pandemic. Mechanical ventilation regiments are still utilized to support declining lung function, but they also contribute to lung damage and increase the risk of bacterial infection. The anti-inflammatory and pro-regenerative abilities of mesenchymal stromal cells (MSCs) have shown to be a promising therapy for ARDS. We propose to utilize the regenerative effects of MSC secretome and the extracellular matrix (ECM) into a nanoparticle. Our mouse MSC (MMSC) ECM nanoparticles were characterized using size, zeta-potential, and mass spectrometry …