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Articles 9541 - 9570 of 19791
Full-Text Articles in Entire DC Network
A Genome-Wide Characterization Of Differentially Expressed Genes Encoding Mrnas And Mirnas And Methylation Analysis Of Phytochrome Genes In A Cotton Phytochrome A1 Rnai Line, Qing Miao
Theses and Dissertations
Silencing phytochrome A1 gene (PHYA1) by RNA interference in upland cotton (Gossypium hirsutum L. cv. Coker 312) had generated PHYA1 RNAi lines with increased fiber length, strength and low micronair (finer fiber). In order to identify and characterize mRNAs and miRNAs that are differentially expressed in the RNAi plants, transcriptome and miRNAome analyses via high-throughput RNA sequencing were performed. Total RNA isolated from 10-DPA (days post anthesis) fibers and small RNAs isolated from 5-, 10-, and 15-DPA fibers of RNAi and Coker 312 lines were used to construct 6 RNA libraries and 18 small RNA libraries, respectively, which were sequenced …
Canine Pure Platelet-Rich Plasma For Regenerative Medicine And Platelet Research: Protocol Optimization, Marina Mitie Monobe
Canine Pure Platelet-Rich Plasma For Regenerative Medicine And Platelet Research: Protocol Optimization, Marina Mitie Monobe
Theses and Dissertations
Platelet-rich plasma (PRP) can be widely used in veterinary medicine in different areas. Studies using PRP frequently use different methodologies making for difficult comparison. The objective of this study was to evaluate the purity and platelet activation of a PRP protocol. A total of 18 blood samples were drawn from six dogs, collected once per week over a total of three weeks. Blood samples were centrifuged six times at 300g for 5 min. Ultra-pure PRP (OP) was obtained by adding PRP a Optiprep 1.063g/mL density barrier and centrifuged at 350g for 15 min. Mean platelet recovery from whole blood was …
Characterization And Value-Added Utilization Of The Proteins Extracted From The By Products Of Catfish Fillet Processing Plant, Haoran Gao
Theses and Dissertations
Proteins in catfish by-products were extracted by two methods: Alkaline extraction (AE) and salt extraction (SE). Properties of the fish protein isolate (FPI) were measured by protein yield and content, moisture content, SDS-PAGE protein patterns, color and texture profile, and compared with commercial surimi products. Our results showed that catfish frame had higher protein yield and color similarity with commercial products than the head; AE-FPI had higher yield and gel strength than SE-FPI; SDS-PAGE protein patterns of FPI from catfish frame by SE method was comparable with commercial surimi products. Based on the results, further optimization of the recovery yield …
Co-Infection Dynamics Of The Tick-Borne Bacteria: Rickettsia Parkeri And "Candidatus Rickettsia Andeanae", Jung Keun Lee
Co-Infection Dynamics Of The Tick-Borne Bacteria: Rickettsia Parkeri And "Candidatus Rickettsia Andeanae", Jung Keun Lee
Theses and Dissertations
Amblyomma maculatum Koch (Acari: Ixodidae) is the primary vector for Rickettsia parkeri which is a causative agent of American Boutonneuse fever. Infection rates of R. parkeri in A. maculatum populations from the United States are approximately 0-56%. The first human clinical case of Rickettsia parkeri infection was reported in 2014 and the patient presented with a febrile illness similar to Rocky Mountain spotted fever but showing milder clinical symptoms. Through 2016, approximately 40 human clinical cases of R. parkeri infection have been reported within southern United States. Amblyomma maculatum may also be infected with a rickettsia of unknown pathogenicity “Candidatus …
Conversion Of Industrial Waste And Wastewaters Into Lipids Suitable For Biodiesel Production, Marta Amirsadeghi
Conversion Of Industrial Waste And Wastewaters Into Lipids Suitable For Biodiesel Production, Marta Amirsadeghi
Theses and Dissertations
The potential of oleaginous yeast Rhodotorula glutinis for the sustainable production of biodiesel feedstock via fermentation of lignocellulosic biomass in pulp and paper wastewater as a fermentation media was investigated. The overall objective was to increase the levels of lipid feedstock in oleaginous yeast in order to reduce biodiesel production cost. To meet sustainable production of biodiesel, industrial wastewater and waste lignocellulose biomass were used as cultivation media and carbon source, respectively. Pulp and paper wastewater effluent was selected as a source of water and nutrients for the production of microbial lipids due to its environmental pollution as it creates …
Copper Coordination With Protein, Peptides, And Small Molecules, Whitnee Leigh Nettles
Copper Coordination With Protein, Peptides, And Small Molecules, Whitnee Leigh Nettles
Theses and Dissertations
Copper is an essential element for all living organisms. However, due to its low redox potential it can be involved in the production of reactive oxygen species; where excess amounts of copper can be exceptionally toxic.1 In humans, malfunctions in copper metabolism are linked to diseases such as Menkes syndrome, Wilson’s disease, prion disease, and Alzheimer’s disease.2 Maintenance of copper homeostasis requires a number of proteins, such as copper transporters and chaperones to deliver copper to the correct protein while limiting free copper in the cell.3 Therefore, understanding the thermodynamics of copper(II) coordination in proteins is critical to our understanding …
Identification And Genetic Characterization Of Antimicrobial Activities Of Pseudomonas Mississippiensis, A Novel Bacterial Species Isolated From Soybean Rhizosphere, Jiayuan Jia
Theses and Dissertations
An aerobic, Gram-negative, rod shaped and polarlagellated bacterium, designated strain MS586, was isolated from soybean rhizosphere in Mississippi. The taxonomic position of MS586 was determined using a polyphasic approach. Analysis of the housekeeping genes supported the novel position of MS586. The results were also supported by average nucleotide identity (ANI) values. Based on these data, it is proposed that strain MS586 represents a novel species, Pseudomonas mississippiensis, within the genus Pseudomonas. The type strain is MS586. Strain MS586 showed a broad-spectrum of antimicrobial activity against plant pathogenic bacteria and fungi that are economically important in agriculture. Preliminary studies using transposon-based …
Identification Of Histamine Receptors In The Canine Gastrointestinal Tract, Alyssa Martin Sullivant
Identification Of Histamine Receptors In The Canine Gastrointestinal Tract, Alyssa Martin Sullivant
Theses and Dissertations
The role of histamine in chronic gastrointestinal diseases has been increasingly recognized in humans, but the role of histamine in the canine gastrointestinal tract has not been thoroughly investigated. The presence and distribution of all 4 histamine receptors (H1, H2, H3, and H4) in the stomach, duodenum, ileum, jejunum, and colon of healthy dogs were evaluated with a commonly employed immunohistochemistry technique using antibodies predicted to cross react with canine histamine receptors. All 4 histamine receptors were identified in the canine gastrointestinal tract, and differed in location and density within sections of the canine gastrointestinal tract. Antibody specificity was evaluated …
Impact Of Simulated Corn Earworm Damage On Field Corn Yield And The Influence Of Chlorantraniliprole And Flubendiamide On Fall Armyworm And Agronomic Characteristics Of Field Corn, Bryan Michael Olivi
Impact Of Simulated Corn Earworm Damage On Field Corn Yield And The Influence Of Chlorantraniliprole And Flubendiamide On Fall Armyworm And Agronomic Characteristics Of Field Corn, Bryan Michael Olivi
Theses and Dissertations
The introduction of pyramided Bacillus thuringinesiss (Bt) in field corn, Zea mays L. has helped reduce kernel and foliar damage from caterpillar pests including fall armyworm, Spodoptera frugiperda (J.E. Smith) and corn earworm, Helicoverpa zea (Boddie). These pests can also be controlled with diamide insecticides. No compensation for manual kernel damage occurred at any section of the ear for Bt or Non-Bt corn. No consistent impacts on corn growth or yield were observed following foliar applications of diamide insecticides at various growth stages. The diamides exhibit longer residual efficacy than other insecticides available for fall armyworm management in field corn. …
Methodology Development For The Measurement And Analysis Of Sediment Oxygen Demands And Nutrient Releases, Luis Alfonso Laurens Vallejo
Methodology Development For The Measurement And Analysis Of Sediment Oxygen Demands And Nutrient Releases, Luis Alfonso Laurens Vallejo
Theses and Dissertations
Sediment oxygen demand (SOD) and nutrients releases from the bottom sediments of a water body are important parameters to be included in studies of water quality processes. Two methods to measure SOD, in situ and at laboratory, were applied at Eckie’s Pond on late spring of 2015. Based upon preliminary results, which showed greater values for the in situ method, some modifications of procedures and equipment were made to improve the measurements. Another set of measurements were made on June of 2016, however their results were not conclusive to establish a correlation between these methodologies. As a result of this …
Role Of Selenium In Age-Related Degeneration: Selenotranscriptome Hierarchy And Characterization Of Selenoprotein H, Lei Cao
Theses and Dissertations
Selenium (Se) is an essential trace element exerting its biological functions mainly through selenoproteins. Our lab has recently shown a paradoxical role of dietary Se at nutritional levels of intake in the delay of age-related degeneration while reducing longevity in mice carrying humanized telomeres. The first aim of this dissertation was to evaluate the effects of long-term dietary Se deficiency, aging, and sex on selenotranscriptome hierarchy in tissues. Four unique patterns in selenotranscriptomic changes were summarized. First, the responses of selenotranscriptomes to dietary Se deprivation and aging were sexually dimorphic. Second, a few selenoproteins responded to dietary Se deficiency and …
A Theoretical Analysis Of Multiproduct Mergers: Application In The Major Meat Processing Sectors, Benjamin Lee Sanderson
A Theoretical Analysis Of Multiproduct Mergers: Application In The Major Meat Processing Sectors, Benjamin Lee Sanderson
Theses and Dissertations
The research is motivated by the significant increase in multiproduct mergers in the meat-protein processing sector, whereby the largest firms now process beef, pork, and chicken. This thesis conducts a theoretical merger analysis, accounting for both within- and across-submarket substitution of demand related goods. The model developed is suitable for analyzing markets in which there are identifiable consumer submarkets within a larger market. The results indicate two primary findings. The first finding is that Bertrand firms have a unilateral incentive to merge. Firms involved in a given merger increase profit, as well as those not included in the merger. Second, …
Strategies For Establishment And Low Input Forage Production Of Southeastern Wildrye, Matthew Thomas Thornton
Strategies For Establishment And Low Input Forage Production Of Southeastern Wildrye, Matthew Thomas Thornton
Theses and Dissertations
Southeastern wildrye (Elymus glabriflorus (Vasey ex L.H. Dewey) Scrib & C.R. Ball) is a relatively new perennial cool-season grass that has shown potential as a forage crop in the Southeastern United States. However, there is a lack of research on this species assessing its agronomic performance. To assess the agronomic performance and it’s tolerance to imazapic field trials were established in Newton and Starkville, MS. A forage production trial included five nitrogen (N) 0, 56, 112, 168, and 224 kg N ha -1 (high input) and five annual clover (low input) treatments. Tolerance to imazapic was assessed with applications of …
Mental Illness In Starkville, Ms: A Cultural Consensus Analysis Of The Public Conceptions Of Mental Illness, Curtis Andrew Kennett
Mental Illness In Starkville, Ms: A Cultural Consensus Analysis Of The Public Conceptions Of Mental Illness, Curtis Andrew Kennett
Theses and Dissertations
Mental illness is a complex phenomenon that is social and psychological as well as biological. But since the creation of the DSM-III in the 1980s, the landscape of mental health research and treatment in the United States has been heavily influenced by the biomedical model. The thoughts and beliefs of the lay public about mental illness are often ignored despite the push for greater cultural understanding among biomedical professionals. This disconnect, coupled with the poor mental health infrastructure, has left Mississippi with an inadequate ability to help Mississippians address mental illness. This research uses cognitive anthropological methods and biocultural theory …
Mississippi Sex Educators' Perceptions Of Youth Sexuality, Sarah Elizabeth Pellegrine
Mississippi Sex Educators' Perceptions Of Youth Sexuality, Sarah Elizabeth Pellegrine
Theses and Dissertations
School-based sexuality education (SBSE) is an important and debated part of the sexual socialization of young people in the US. While existing literature addresses the sociological implications of SBSE at the policy and curriculum-level, little was previously known about the ways instructors carried out and made sense of sex education in their classrooms. In this study, I examine the relationship between how sex education instructors make sense of sex education and their understandings of youth and sexuality. I conducted 20 semi-structured interviews with sex education teachers in Mississippi public schools and used an inductive analysis approach to determine themes from …
Relationships Among Environmental Attitudes, Environmental Knowledge, And Outdoor Recreational Habits Of Upper Elementary School Students In Mississippi, Katherine Elizabeth Abell
Relationships Among Environmental Attitudes, Environmental Knowledge, And Outdoor Recreational Habits Of Upper Elementary School Students In Mississippi, Katherine Elizabeth Abell
Theses and Dissertations
Research sought to evaluate environmental attitudes, environmental knowledge, and nature-based recreation in Mississippi upper-elementary adolescents, as delineated by race/ethnicity and gender. Research was conducted Spring 2015 in eight study schools using three survey instruments. Analysis was conducted using one-way ANOVA, univariate analysis of variance, and generalized linear mixed models. Results found that no gender differences exist between environmental attitudes or knowledge; however, Caucasian/White students displayed significantly higher environmental knowledge and attitudes versus their African American/Black peers. Additionally, a positive relationship was detected between attitudes and knowledge of study participants. Outdoor, nature-based recreation participation varied along race/ethnicity and gender, with Caucasian/White …
Spatial And Trophic Niche Specialization In Castor Canadensis, Robert Antonio Francis
Spatial And Trophic Niche Specialization In Castor Canadensis, Robert Antonio Francis
Theses and Dissertations
The Hutchinsonian niche is the n dimensional hyper volume that allows for the persistence of a species. Castor canadensis, a large semi-aquatic rodent, is an ecosystem engineer and often a keystone species for many ecosystems. I examined the effect of multiple spatial scales on hierarchical habitat selection byC. canadensis using presence-only modeling techniques. I also determined individual trophic niche specialization in C. canadensis utilizing stable isotope analysis. I concluded that C. canadensis displayed scale independent habitat selection when comparing landscape and fine spatial scales. Individual trophic niche specialization occurred in colonies of the same resource availability. Also, individual trophic niches …
Evaluation Of Urea Hydrolysis As A Biomarker For Detecting Pathogenic Vibrio Parahaemolyticus In Clinical Isolates And Raw Oysters, Maryam Kate Mohammadi-Aragh
Evaluation Of Urea Hydrolysis As A Biomarker For Detecting Pathogenic Vibrio Parahaemolyticus In Clinical Isolates And Raw Oysters, Maryam Kate Mohammadi-Aragh
Theses and Dissertations
Vibrio parahaemolyticus (Vp) infection is commonly caused by the consumption of raw or undercooked shellfish. Raw oysters are associated with most Vp outbreaks. Pathogenic Vp express thermostable-direct hemolysin (tdh) and to some extent thermostable-related hemolysin (trh). Additionally, some pathogenic Vp express urease (uh). The objectives of this work were to discern any relationships between urease (uh) expression, tdh/trh expression, and hemolytic activity in pathogenic and non-pathogenic clinical strains, and to compare urease, motility, tdh/trh expression, and hemolytic activity in raw oyster isolates. This information would determine if urease could be used as a biomarker to detect pathogenic Vp. About 80% …
Gene Expression Profiling Of Wood Decay Fungus Fibroporia Radiculosa Grown On Different Organic And Copper Based Preservatives, Ayfer Akgul
Theses and Dissertations
Copper tolerant brown rot fungi are able to depolymerize the structure of wood treated with copper or organic wood preservatives. This research used quantitative polymerase chain reaction (qRT-PCR) combined with RNA-seq to explore what genes of the brown-rot fungus, Fibroporia radiculosa, are expressed when the fungus is overcoming the wood preservatives and decaying the wood. The preliminary study of ACQ-treated wood indicated that the hydrogen peroxide needed for wood decay to proceed may come from AAOX (aryl alcohol oxidase), with oxalate regulation by ODC2 (oxalate decarboxylase), and copper regulation by COP (copper resistance P-type ATPase pump). The principal study measured …
Reproductive Ecology Of Greater Sage-Grouse In Strawberry Valley, Utah, Jared Jeffrey Baxter
Reproductive Ecology Of Greater Sage-Grouse In Strawberry Valley, Utah, Jared Jeffrey Baxter
Theses and Dissertations
Greater sage-grouse (Centrocercus urophasianus; hereafter, sage-grouse) are a species of conservation concern in the rangelands of western North America due to their dramatic decline over the last half century. Effective conservation and management of sensitive species requires an understanding of how species respond to management actions. We examined two aspects of the reproductive phases of sage-grouse: nest predation, and habitat selection by female sage-grouse with chicks. In Chapter 1, we developed resource selection functions to assess the influence of mechanical treatments of mountain big sagebrush (Artemisia tridentata vaseyana) on habitat selection by greater sage-grouse with chicks. …
Comparison Of Simulation And Hands-On Labs In Helping High School Students Learn Physics Concepts, Matthew Charles Rytting
Comparison Of Simulation And Hands-On Labs In Helping High School Students Learn Physics Concepts, Matthew Charles Rytting
Theses and Dissertations
The purpose of the research was to determine whether PhET simulation labs or hands-on labs were more effective in helping students learn physics concepts. This measure was done by comparing quiz scores using recall, calculation, and transfer questions. Additionally, student perceptions of learning from both hands-on and simulation lab experiences were measured. Six labs were conducted with high school physics students on the topics of momentum, energy, circuits, angular momentum, pendulums, and friction. It was found that PhET simulation labs were as effective at creating student understanding, and sometimes more effective, as measured by quizzes given after the labs. Additionally, …
The Role Of Viral Interleukin-6 In Tumor Development Of Kaposi's Sarcoma-Associated Herpesvirus Lymphomas, Rebecca A. Fullwood
The Role Of Viral Interleukin-6 In Tumor Development Of Kaposi's Sarcoma-Associated Herpesvirus Lymphomas, Rebecca A. Fullwood
Theses and Dissertations
Kaposi's sarcoma herpesvirus (KSHV) is a cancer-causing virus, primarily affecting AIDS patients. KSHV is found in 3-10% of the U.S. population and can cause a range of cancers in the highly immunosuppressed; these cancers include Kaposi's sarcoma, pleural effusion lymphoma (PEL) and multicentric Castleman's disease (MCD). The current techniques for treating these cancers are relatively ineffective, largely due to their inefficiency at targeting tumors formed by the infection. One protein produced by KSHV, the viral homolog of interleukin-6 (vIL-6), is thought to play a major role in tumor development post-infection. Here a novel animal model is implemented to study the …
Nanoemulsions Within Liposomes For Cytosolic Drug Delivery To Multidrug-Resistant Cancer Cells, Jacob Brian Williams
Nanoemulsions Within Liposomes For Cytosolic Drug Delivery To Multidrug-Resistant Cancer Cells, Jacob Brian Williams
Theses and Dissertations
Cancer cells that survive chemotherapy treatment often develop resistance to the administered chemotherapeutics, as well as to many other types of drugs, because the cancer cells increase their production of efflux pumps in the cell. This undesired phenomenon of resistance to cancer drugs is known as multidrug resistance. This work uses a novel drug carrier, called an eLiposome, to achieve cytosolic drug delivery to kill multidrug-resistant cancer cells. An eLiposome consists of a perfluoropentane (PFC5) emulsion droplet inside of a liposome. Folate attached to the eLiposome facilitates uptake into the cell. The PFC5 droplet is metastable at body temperature, but …
Topic Manipulation In Five Children With Language Impairment In Response To Topic Probes, Kimberly Kasey Baker
Topic Manipulation In Five Children With Language Impairment In Response To Topic Probes, Kimberly Kasey Baker
Theses and Dissertations
This study describes a series of case studies on topic management patterns of five children (ages 5 to 10 years) with language impairment. The children participated in semi-structured topic tasks that assessed conversational abilities on topics that were verbally introduced and topics that were introduced both verbally and with an object. Although there was considerable variability among participants, the children generally responded to most introductions by acknowledging and maintaining the topic. With the exception of one child, however, the children in this study demonstrated immature topic manipulation patterns that could be expected to have negative social ramifications.
Carbon And Nitrogen Dynamics Of Anthropogenically Disturbed Seagrass Ecosystems, Alison Shepherd
Carbon And Nitrogen Dynamics Of Anthropogenically Disturbed Seagrass Ecosystems, Alison Shepherd
Theses and Dissertations
As atmospheric CO2 concentrations continue to rise, understanding factors that influence size and longevity of carbon (C) pools within coastal and marine ecosystems (i.e., “blue carbon”) is critical. In 2013, the global average atmospheric CO2 concentration reached 396 ppm, which was the highest recorded level in the last 800,000 years. Recent research acknowledges the importance of “blue carbon” in the global C cycle, finding that these ecosystems capture up to 70% of organic C in the marine realm, rendering them one of the most efficient C sinks on the planet. Specifically, seagrass ecosystems are critically important to the global C …
Avian Communities In An Urbanizing Region: Abundance Patterns And Effects Of Local Habitat Features, John S. Brush
Avian Communities In An Urbanizing Region: Abundance Patterns And Effects Of Local Habitat Features, John S. Brush
Theses and Dissertations
The Lower Rio Grande Valley of Texas has experience rapid population growth and increase of urban area since the mid-20th century. The goal of this thesis were to: 1) explore patterns of avian communities in suburban and remnant urban woodlands, with particular interest in native forest species, and 2) to assess how local habitat variables, such as number of native trees, affect avian communities in suburban habitats, and 3) provide recommendations to municipalities and homeowners to enhance urban habitats for birds. Results indicated that remnant urban woodlands provided habitat for common native forest bird species that otherwise avoided suburban habitats, …
Developing A Multimetric Index To Assess Resaca Ecosystem Health, Buford J. Lessley
Developing A Multimetric Index To Assess Resaca Ecosystem Health, Buford J. Lessley
Theses and Dissertations
As the only freshwater ecosystem in the lower Rio Grande Valley aside from the Rio itself, resacas are critical habitat for many species of flora and fauna. Old distributaries of the Rio Grande, resacas provided conveyance routes moving floodwater to the Laguna Madre. Today these wetlands are novel ecosystems and are artificially maintained. Urbanization and agriculture have lead to sedimentation, habitat loss, contaminants, poor water quality, and invasive species. The objective of this study was to assess and monitor resaca pools and to compose the Resaca Health Index (RHI) from selected indicators of ecosystem structure and function including leaf litter …
Parental Experience-Based Change: Positive And Negative Changes In Monitoring, Expectations, Nurturing, And Discipline, Joseph S. Rand
Parental Experience-Based Change: Positive And Negative Changes In Monitoring, Expectations, Nurturing, And Discipline, Joseph S. Rand
Theses and Dissertations
This study sought to create a measure of parent's perceptions of parental experience-based change (PEBC), or parents' perceptions of the changes they make to their parenting of secondborn children as a result of experiences with firstborn children. The measure assessed PEBC in the domains of monitoring, expectations, nurturing and discipline. Participants were 401 mothers or fathers of 2 or 3 adolescent children. Factor analyses revealed an 8 factor solution that assessed increasing and decreasing in each of the 4 domains. Criterion validity was evaluated using regression analyses to examine the relationships between each factor and parenting outcomes thought to be …
Analysis Of Nucleosome Isolation And Recovery: From In Silico Invitrosomes To In Vivo Nucleosomes, Collin Brendan Skousen
Analysis Of Nucleosome Isolation And Recovery: From In Silico Invitrosomes To In Vivo Nucleosomes, Collin Brendan Skousen
Theses and Dissertations
There are a vast number of factors that influence nucleosome formation, and consequently gene regulation. These factors include histone modifications, nucleotide composition, transcriptional region elements, and specific nucleotide motifs, among others. Although the amount we know now is limited, we are creating new techniques and discoveries to assist us in continued understanding of chromatin. To make a significant contribution to the field of chromatin, I conducted two hypothesis driven sets of experiments that address the topic of chromatin structure. First, I created a technique for tissue specific nucleosome isolation with the goal of observing the effect of single nucleotide polymorphisms …
Gbann-V: Genetically Based Artificial Neural Network With Validation For Optimal Feature Selection And Data Mining, Eric W. Worden
Gbann-V: Genetically Based Artificial Neural Network With Validation For Optimal Feature Selection And Data Mining, Eric W. Worden
Theses and Dissertations
In data mining and machine learning systems, highly predictive feature attributes help researchers and data analysts classify the problem space in the most optimal way. In this thesis paper, a feature subset selection classifier system for data mining and machine learning will be presented. This feature subset selection system will test data from the PIMA Indian Diabetes Data and the Breiman Waveform Data Set. The system collects the input features and then processes them through an evolutionary search process in order to reduce the feature space into an optimal feature set. Reducing irrelevant attributes can decrease the complexity and processing …