Digital Commons Network^™

Development Of A Qualitative Spot Assay For Zinc Detection In Fortified Cereal Grain Matrices, Ashley L. Lamborn

Theses and Dissertations

The present thesis is a compilation of a literature review, journal manuscript, and two standard operating procedures (SOP) on the qualitative determination of zinc in fortified cereal grain products and matrices. The literature review addresses the premise of the research as well as potential solutions for the development of a rapid qualitative spot assay for zinc determination. Quality assurance assays affirm the presence of micronutrients in food fortification initiatives, but no rapid qualitative spot methods for the assessment of zinc currently exist. Chromogenic reagents that may perform a key role in the rapid determination of zinc are identified. The journal …

Analysis Of Nitrogen Accumulation And Gene Expression In Hydroponically-Grown Chenopodium Genotypes, Teal Kalt

Theses and Dissertations

Nitrogen (N) is an essential macronutrient required by all plants to complete their life cycles. Inefficient N uptake contributes to over fertilization and subsequent environmental and sustainability issues resulting in the need for improved nitrogen use efficiency (NUE). Chenopodium quinoa is a resilient pseudocereal crop that is relatively adapted to N-deficient soils. Previous studies have uncovered the unique genetic structure of quinoa and its physiological responses to N availability; however, gaps remain in understanding its molecular mechanisms for NUE. This study investigates four quinoa genotypes (Real-1, 0654, KU-2, and QQ74) hydroponically grown in N-deficient conditions. RNA sequencing (RNA-seq) analysis identified …

Using Human Computation And Harmonic Expansions For Characterization And Design Of Grain Boundary Networks, Christopher W. Adair

Theses and Dissertations

Grain Boundary Networks (GBNs) are a high-dimensional feature in polycrystalline microstructural materials that are formed from the crystallographic degrees of freedom. This feature affects multiple material properties such as diffusivity, fracture, elasticity, heat transfer, and others. The link between the crystallography and the material properties is called the structure-property linkage, and allows for simulation and design of materials. While it is possible to use the structure-property linkage to design materials for better performance, the high-dimensional nature of the interconnected grain boundaries preclude the use of many common optimization algorithms for efficiency or local minima considerations. This work shows how the …

"Because He Was A Cripple": Collective Care And The Theory Of Complex Embodiment In Dickens's A Christmas Carol, Briley Wyckoff

Theses and Dissertations

Published in 1843, Charles Dickens's A Christmas Carol planted itself squarely into a society that depended upon physical labor and valued able-bodiedness. The crutch-bearing Tiny Tim is iconized for his angelic nature and positive outlook on his impairment, prompting critical conversation about narrative prosthesis and poster children, yet the other illustrations of disability in the novella are rarely explored. Contrary to the tendency of disability scholars to conclude that A Christmas Carol leans too heavily into the trope of inspirational disability or diminishes disabled agency, Dickens's incorporation of disability actually extends far beyond Tim as each member of the Cratchit …

Investigating The Effects Of Sex And Estrous Cycle On The Incubation Of Cue-Induced Oxycodone Craving, Bhumiben Pradipkumar Patel

Theses and Dissertations

The overprescription and misuse of opioids like oxycodone has played a significant role in the ongoing opioid crisis. While effective painkillers, drugs like oxycodone are also reinforcing and often misused. While sex and ovarian hormones can impact the analgesic effects of these drugs, the impact of these factors on oxycodone reinforcement are not fully understood. This relationship between ovarian hormones and opioid reward is further complicated by the fact that opioid use can disrupt ovarian hormone levels and produce reproductive cycle irregularities. Using a rat model of drug craving and relapse vulnerability, we found that chronic oxycodone exposure produced reproductive …

Toxiverse: An Online Comprehensive Chemical Profiling, Modeling, And Data Analysis Platform, Yitao Shen

Theses and Dissertations

Massive chemical data is now publicly available through publications and various online databases. However, most online databases only provide data unsuitable for modeling purposes and limited machine learning (ML) modeling for those data. As a public chemical toxicity evaluation tool to resolve these issues, the Toxicology Universe (ToxiVerse) portal hosts curated chemical structures and toxicological/pharmaceutical data of 26,000 compounds which can be used directly for modeling purposes. Furthermore, users can upload chemical datasets to extract all involved bioassays for compounds inside as biological profiles. ToxiVerse also features automatic QSAR modeling of uploaded datasets using multiple chemical descriptors and algorithms provided …

Contingency Management For Opioid Use Disorder Buprenorphine Treatment Engagement: 3-Month Outcomes, Shelby Renee Goodwin

Theses and Dissertations

Individuals with opioid use disorder exhibit difficulty sustaining prolonged buprenorphine treatment engagement. Contingency management (CM; incentives) may serve to increase the reinforcing value of treatment itself, but most buprenorphine CM studies delivered incentives more frequently than in typical outpatient practice. This study sought to compare CM with treatment as usual (TAU; regular clinic procedures) in visit attendance and adherence (defined as urine samples positive for buprenorphine and negative for other opioids) in new outpatients at a hospital opioid treatment clinic. Participants (46 per group) were randomized to either CM or TAU and monitored by medical record for 12 weeks; for …

Measures Of Verbal Comprehension Patterns In Autistic Females, Savanna Leigh Parady

Theses and Dissertations

Although a rapidly growing body of scientific literature is leading to more understanding of autistic traits in women and girls, their diagnosis continues to occur at later ages. Diagnosis received at later ages limits access to early intervention and related positive outcomes. The purpose of this study was to add data to the weight of evidence approach in differential diagnosis of autism in females and other underserved groups. Specifically, I set out to identify patterns between verbal comprehension tasks measuring vocabulary knowledge (crystallized knowledge) and analogous verbal comprehension (similarities) tasks drawing on additional cognitive abilities. Verbal analogy tasks measure knowledge …

Elucidating The Antibiofilm Characteristics Of A Carbon Infiltrated Carbon Nanotube Surface And A Copper-Coated Carbon Infiltrated Carbon Nanotube Surface, Lucy Christena Bowden

Theses and Dissertations

Post-surgical implant-associated infections cause considerable patient morbidity and mortality. These infections are often caused by biofilm-forming bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. Bacterial biofilms are resistant to immune clearance and are a reservoir of antibiotic resistance. Implant materials that resist bacterial colonization may be able to improve patient outcomes. Carbon infiltrated carbon nanotubes (CICNT) are a potential surface modification for medical implants. The work presented in this dissertation focuses on characterizing the antibiofilm effect of the CICNT surface, better understanding its mechanism of action, and characterizing the enhanced antibiofilm effect of a copper-coated CICNT surface. S. aureus biofilms …

Investigation Of The Hdl Molecular Architecture Before And After Exercise Training., Prasun Kumar Dev

Theses and Dissertations

The functional characteristics of high-density lipoprotein (HDL) particles are influenced by their structure, including their molecular composition (e.g. HDL lipidome and proteome). However, the effects of exercise training on HDL molecular composition and its relationship with HDL-related traits are largely unknown. In this ancillary study, we investigated the HDL lipidome and proteome of 154 adults before and after 20 weeks of exercise training in the HERITAGE Family Study. The HDL-sized plasma fraction was isolated utilizing FPLC-SEC, followed by untargeted lipidomic and proteomic analysis using LC/MS. We identified 341 known lipid species while 409 HDL associated proteins. Clinical and functional related …

Barriers In Receiving A Diagnosis Of Sanfilippo Syndrome: Perceptions From Healthcare Providers And Caregivers, Cassidy Lena

Theses and Dissertations

Sanfilippo syndrome (MPS III) is a rare, degenerative condition characterized by symptoms impacting neurological functioning, behavior, and quality of life. Diagnosis is often not made until three to six years of age, but comprehensive and effective symptom management have been reported to optimize patient longevity. The aim of this study was to identify barriers to diagnosis and the corresponding impact on patients. This study surveyed healthcare providers and caregivers of individuals with Sanfilippo syndrome. Both quantitative and qualitative methods were employed to assess provider knowledge and comfortability in managing Sanfilippo syndrome. Additionally, it explored caregiver perspectives on healthcare system navigation, …

Assessing The Feasibility Of Transportable Nuclear Reactors: A Radiological Risk Case Study, Robert John Demuth

Theses and Dissertations

The feasibility of a truly transportable nuclear reactor presents significant radiological challenges that must be addressed to ensure compliance with regulatory limits and operational safety. This study evaluates the radiological constraints associated with the transportability of the Transportable Helium-cooled One-megawatt Reactor (THOR), focusing on neutron activation, shielding performance, and external radiation exposure. Using advanced radiation transport and activation modeling within the SCALE framework, a comprehensive analysis was conducted to quantify dose rates during operation, post-shutdown, and transport. A key aspect of this work involved assessing activation products in core materials, auxiliary components, and surrounding environments to determine their contribution to …

Activation Of Basal Forebrain Afferents Following Systemic Inflammation, Alexis Morgan Blas

Theses and Dissertations

Sepsis is a large-scale immune response typically due to an imbalance of pro- and anti-inflammatory responses due to some type of infection or insult. Around 1.7 million adults in the United States will develop it each year, making it the leading cause of increased morbidity and mortality in hospitals. Sepsis can lead to many different symptoms, one of which is delirium. Delirium is a mental state of confusion and disorientation and involves delusions, hallucinations, memory issues, and most commonly inattention. Delirium is more prevalent in older individuals which leads to further complications and a higher likelihood of development of long-term …

Integrating Traditional Chinese Aesthetic Culture With Contemporary Music Through Violin, Fan Kai-Yi Zhang

Theses and Dissertations

This dissertation investigates the integration of traditional Chinese aesthetic principles with contemporary music through selected violin repertoires. It examines the rich tradition of Chinese aesthetics, emphasizing the distinctive musical styles that, despite their variations, share a common cultural foundation. The research combines a comprehensive review of classical Chinese literature, musicological analysis, exploration of pitch materials, harmonic language, and performance-based interpretation to blend and expand the technical and expressive possibilities of the violin with the essence of Chinese music. Rooted in an analysis of key traditional Chinese aesthetic concepts—he (harmony), tianren heyi (the unity of nature and humanity)—and the two major …

Adolescent Sleep Health, Mental Health, And Cardiovascular Disease Risk In Adulthood, Prince Nii Ossah Addo

Theses and Dissertations

Background: Insufficient sleep and poor sleep quality are major public health issues among adolescents in the United States (U.S.). Mental health and substance use challenges are also common among adolescents, particularly those with sleep problems. Although exposures in adolescence have been evidenced to significantly impact future health, little is known about how adolescent sleep health, mental health, and substance use influence future cardiovascular disease (CVD) risk. We, therefore, examined the association between sleep health in adolescence and CVD risk in adulthood and assessed whether the association is mediated by depressive symptoms and substance use in adolescents. Further, we explored the …

Understanding Water Washing Needs Of Hydrochar Generated From The Hydrothermal Carbonization Of Food Wastes To Establish Minimum Post-Processing Needs For Soil Amendment Application, Neve Renee Steger

Theses and Dissertations

Hydrothermal carbonization (HTC) is an advantageous and potentially environmentally beneficial approach for the conversion of wet biomass and waste streams into a value-added solid product referred to as hydrochar. Although hydrochar composition and properties generated from the carbonization of a variety of feedstocks have been well studied, there is a need for understanding whether hydrochar requires further refinement and/or treatment prior to being used in environmental applications, particularly when using the hydrochar as a soil amendment. Understanding the need for and extent of hydrochar post-treatment is critical in identifying environmentally beneficial and economically attractive strategies for hydrochar use. In this …

Phytochemicals And Human Health, Erin Kelley Richards

Theses and Dissertations

This review explores the intricate relationship between phytochemical intake and human health. It first highlights the contrast between the phytochemical-poor Standard American Diet (SAD) and ancestral diets rich in diverse plant and animal foods. The classification, sources, and health benefits of various phytochemicals, including polyphenols, flavonoids, alkaloids, and terpenes are described and discussed. The bioavailability of these compounds and their interactions with the gut microbiome are elucidated, emphasizing on their potential in modulating multiple pathways associated with aging and chronic diseases. The 12 hallmarks of aging and how phytochemicals may influence these hallmarks are reviewed. While exploring the potential benefits …

The Association Between Parent’S Mobile Device Use And Physical Activity, Sydney Hill

Theses and Dissertations

Background. The rapid increase in mobile technology has allowed smartphones to be used almost anywhere, but a potential disadvantage of these mobile devices is the displacement of physical activity. Parents of young children are already struggling to reach higher intensities of physical activity and mobile devices might make this worse. However, most existing research on similar topics look at adults without children or uses subjective measures only. Therefore, this study aims to (1) quantify objective mobile device use from a sample of parents with preschool age children; (2) describe the duration and intensity of parents’ physical activity and inactive behavior; …

An Exploratory Study Of Disclosure Of X-Linked Conditions Between Mothers And Daughters, Madge Isabella Stuhlreyer

Theses and Dissertations

Carriers of X-linked conditions, both asymptomatic and symptomatic, face unique challenges regarding their experiences navigating the healthcare system and understanding implications of their carrier status. Studies show that mothers are known to be the main communicators of genetic information within their families, but in families affected with X-linked conditions, communication between mothers and daughters are hindered by factors such as lack of knowledge about the condition and reproductive implications, lack of emotional support, anxiety, and uncertainty about how to initiate these conversations. The purpose of this study was to explore the motivations behind mothers' decisions to initiate conversations with their …

Increasing Diversity In The Genetic Counseling Profession: Determining Effective Career Education Strategies For Underrepresented Youth, Bryel Marie Frasch

Theses and Dissertations

Genetic counseling is a professional career path that is lacking in diversity, and diversity in healthcare has been shown to improve health outcomes. Studies suggest student engagement as a way to increase diversity, as early knowledge of genetic counseling increases the likelihood of considering it as a career and is especially true for racial or ethnic minoritized students. This study focused on educating high school students underrepresented in genetic counseling about the career and exploring the effectiveness of two different types of education methods (video vs in-person) to give valuable information for future outreach efforts. Upward Bound and Federal TRIO …

Associations Between Cardiovascular Disease And Utilization Of Dental Preventive And Periodontic Services, Amirhossein Fakhre Yaseri

Theses and Dissertations

Cardiovascular disease (CVD), including coronary artery disease, heart failure, myocardial infarction, and stroke, remains a leading cause of global mortality, with approximately 20.5 million deaths in 2021. Despite advancements in medical science, CVD prevalence continues to rise, underscoring the need for preventive approaches and increased awareness. CVD is closely associated with oral diseases, and poor oral health is linked to worse cardiovascular outcomes. This study investigates the association between CVD and the utilization of dental preventive and periodontal services using data from the Dental Experience and Research Exchange (DERE) database, which aggregates clinical data from dental practices across the U.S. …

Reclaiming The Body: Gender Politics And Embodied Meaning In Piano Performance And Pedagogy, Rachel Misheff

Theses and Dissertations

This study examines the impact of the mind-body duality and its gendered metaphor on the lived experiences of historical and contemporary female pianists. Specifically, it explores how the principles of mind-body dualism have constructed gendered practices in piano performance and pedagogy that have both marginalized female pianists and engendered distinct embodied relationships to music. The study draws upon research from other disciplines, such as phenomenology, theories of embodiment, feminist theory, psychoanalysis, and Embodied Music Cognition, to examine how these gendered musical norms resulting from mind-body dualism functioned as forces of social inscription. This research, therefore, suggests that the body’s lived …

Estimating Proximity To Failure Using Repetitions In Reserve In Adults Treated For Cancer, Kylah Elizabeth Jackson

Theses and Dissertations

BACKGROUND: There is a consensus from governing bodies that more evidence is needed in understanding how to properly prescribe, monitor and progress resistance training (RT) in oncology populations. Repetitions in reserve (RIR) has been demonstrated as a quick/easy tool to monitor proximity to failure and interventions using RIR have shown promise in improving muscle strength and mass in older adults and apparently healthy populations. However, the validity of RIR in RT in oncology has yet to be examined. Therefore, the purpose of this study was to assess the accuracy of RIR predications in individuals treated for cancer in a pragmatic …

From Pregnancy To Postpartum: A Longitudinal Analysis Of Maternal Depressive Symptoms And Hemoglobin Concentration In Uttar Pradesh, India, Abdullah Nurus Salam Khan

Theses and Dissertations

Background: Perinatal depression and maternal anemia impact maternal health, pregnancy outcomes, and infant growth in low- and middle-income countries. This study examines the prevalence and risk factors of these conditions during pregnancy and postpartum in rural Uttar Pradesh, India. Methods: A prospective cohort study was conducted among 475 pregnant women recruited during early pregnancy (≤20 weeks gestation) and followed through late pregnancy (≥27 weeks) and postpartum (< 42 days of childbirth). The outcome variables were, i) depressive symptoms, assessed using the Edinburgh Postnatal Depression Scale (EPDS), and ii) hemoglobin concentrations, measured using HemoCue Hb 301 analyzer from capillary blood. Multivariable linear regression examined associations of the outcome variables with sociodemographic, obstetric, nutritional, interpersonal, and perinatal factors. Path analysis evaluated direct and indirect paths influencing postpartum hemoglobin. Results: Prevalence of probable depression (EPDS score≥13) peaked in early pregnancy (24%) and declined in late pregnancy (17%) and postpartum (4%). Mean hemoglobin declined from early (10.5 g/dL) to late pregnancy (9.7 g/dL), and partially recovering in postpartum (10.4 g/dL). Household food insecurity was associated with higher depressive symptoms during pregnancy and postpartum (early pregnancy:β=2.61, p=0.011; late pregnancy:β=2.84, p< 0.001; postpartum:β=1.40, p=0.023). Psychological intimate partner violence was associated with greater depressive symptoms in early pregnancy (β=3.19, p=0.001). Receiving more antenatal visits was associated with decrease in depressive symptoms in late pregnancy (β=-0.46, p=0.001) and hemoglobin at postpartum (β=-0.11, p=0.070). Consumption of more food groups was associated with increase in depressive symptoms (β=0.63, p=0.004) and hemoglobin in late pregnancy (β=0.10, p=0.026). Iron folic acid (IFA) consumption was associated with increase in hemoglobin in late pregnancy (β=0.01, p< 0.001) and postpartum (β=0.004, p=0.031). Experiencing postpartum health complications was associated with increase in depressive symptoms (β=0.41, p=0.013) and decrease in hemoglobin concentrations (β=-0.18, p=0.036). Antenatal hemoglobin concentrations mediated the associations of IFA supplementation and dietary diversity with postpartum concentrations. Conclusion: Perinatal depression was associated with socioeconomic vulnerabilities, food insecurity, and intimate partner violence, while hemoglobin concentrations were associated with maternal nutrition and perinatal care. Integrating mental health services and comprehensive nutrition interventions into antenatal and postpartum care can improve psychological and physical well-being of mothers during and after pregnancy.

Approaches To Teaching Music Reading To Students With Dyslexia: A Guide For Piano Teachers, Olivia Grace Bradstreet Colomaio

Theses and Dissertations

Dyslexia is the most common learning disability in the United States, affecting approximately one in five individuals. According to the DSM-V-TR, the ICD-11, and the International Dyslexia Association, dyslexia is a Specific Learning Disorder that causes issues with phonological processing, sequencing of information and information processing, word recognition, reading fluency, and other issues. Due to its prevalence, the chances of piano teachers encountering a student with dyslexia in their teaching studios is highly likely. However, there is relatively little research available for piano teachers of students with dyslexia. In the music lesson, common areas of struggle for students with dyslexia …

The Role Of B Cells In Promoting Lipid Absorption By Promoting Lacteal Development, Kenny Johnson

Theses and Dissertations

The absorption of dietary lipids in the gut is necessary to maintain the metabolic tone of tissues. Inflammation in the gut can cause defects in lipid absorption, which can result in tissue wasting, otherwise known as cachexia. Cachexia is commonly observed in patients with inflammatory bowel disease and cancer patients. The underlying cause of cachexia is unknown, and there are currently no effective therapeutic options available to treat it. Lymphatic drainage in the small intestine (SI) is specialized to promote the transport of dietary lipids into circulation. Lacteals are blunt-ended lymphatic vessels localized to the central axes of villi in …

A Study To Identify The Causal Rare Genetic Variants In Primary Open And Closed Angle Glaucoma, Pseudoexfoliation Syndrome, And Associated Glaucoma, Suganya K

Theses and Dissertations

BACKGROUND: Glaucoma is the second most common cause of blindness globally typically diagnosed with a triad of clinical symptoms of increased intraocular pressure (IOP) with associated optic disc, optic nerve head (ONH) changes, and visual field defects. Genetic and environmental factors are some of the strong aetiology factors for glaucoma and identification of these factors has a potential implication in the management of the disease and its outcome. There is a paradigm shift towards understanding the genetics of glaucoma, wherein the variants in the nuclear, mitochondrial genome and other regulatory regions are being identified as contributing risk factors.

METHODOLOGY AND …

"Very Important Stuff For The Ladies": Examining Gender Differences In Celebrity Journalism, Payton E. Pingree

Theses and Dissertations

This thesis explores gender differences in celebrity journalism by analyzing 44 YouTube video interviews from the Oscars red carpet between 2014 and 2024. Using framing theory and feminist theory, the study examines how interviewers interact with male and female celebrities, focusing on four key behaviors: infantilizing, ambivalence, sexualization, and heteronormativity. Through qualitative thematic analysis of interview transcripts, the research reveals that female celebrities are often sexualized through questions about their appearance and subjected to heteronormative expectations when discussing their fashion choices and familial roles. These patterns suggest persistent sexism in celebrity journalism, potentially influencing public perceptions of women in the …

Characterizing The Effects Of Rage Upregulation In Lung Development, Derek Michael Clarke

Theses and Dissertations

The Receptor for Advanced Glycation End-products (RAGE) is a transmembrane, multi-ligand cell surface receptor highly expressed in pulmonary tissue, where it plays a critical role in mediating inflammatory responses. Under chronic toxicant exposure, often associated with cigarette, e-cigarette, and diesel particulate, RAGE transitions from a protective to a deleterious state, contributing to pathological processes. In adult models, RAGE's role in the development of lung disease has been well studied, yet in the context of lung development RAGE has not been well studied with a small number of clinical papers finding increased levels of RAGE in bronchopulmonary dysplasia(BPD) and respiratory distress …

Transgenerational Impacts Of Covid-19: Exploring The Epigenetic Impacts Of Sars-Cov-2 Infection On Parental Reproductive Health And Offspring Development, Isaac Mckay Stirland

Theses and Dissertations

The mechanisms and consequences of SARS-CoV-2 infection, including clinical manifestations, immune response, pathogenicity, long-term sequelae, etc., have been studied in detail following the 2019 pandemic however, very little has been done to explore the interplay between SARS-CoV-2 and host DNA methylation. Given the role of DNA methylation in genetic regulation, genomic imprinting, and induction of transgenerational effects, an understanding of viral interaction(s) with the host methylome is imperative in promoting reproductive and developmental health. Numerous reports describe infections with similar pathogenicity to SARS-CoV-2 and their correlation to altered host tissues at the epigenetic, physiologic, and macroscopic level. These reported epigenetic …