Digital Commons Network^™

Development And Evaluation Of A Combinatorial Rt-Qpcr Multiplex For Forensic Body Fluid Identification, Carolyn A. Lewis

Theses and Dissertations

Body fluid identification is essential in the forensic biology workflow that assists DNA analysts in determining where to collect DNA evidence. Current presumptive tests lack the sensitivity and specificity molecular techniques can achieve; therefore, molecular methods, such as microRNA and microbial signatures, have been extensively researched in the forensic community. Limitations of each method suggest combining molecular markers to increase discrimination efficiency of multiple body fluids from a single assay. While microbial signatures have been successful in identifying fluids with high bacterial abundances, microRNAs have shown promise in fluids with low microbial abundance. A disadvantage of RNA analysis in forensic …

Functional Limitations And Well-Being Throughout The Adult Lifespan: The Moderating Role Of Sleep, Claire M. Williams

Theses and Dissertations

Functional limitations represent individuals’ difficulty with completing essential activities of daily living, such as sitting, stooping, and walking. Though functional limitations have been linked to lower well-being outcomes, less is known about potential protective factors for well-being in the experience of functional limitations. The present study used archival data from the MIDUS Refresher study to evaluate how sleep and salient aspects of identity may alter the association between the experience of functional limitations and well-being. In particular, this study had two central aims: to examine the associations between functional limitations, life satisfaction, and affect and detect how aspects of identity …

Microrna In The Mammalian Growth Plate: Matrix Vesicles And 1Α,25-Dihydroxyvitamin D3, Niels C. Asmussen

Theses and Dissertations

Growth plate chondrocytes are the driving force of long bone elongation and they accomplish this vital task, in part, by producing, remodeling, and finally mineralizing the cartilage tissue that makes up the growth plate. This mineralized tissue is turned over again as bone forming cells invade and began the process of turning mineralized cartilage into bone. Chondrocytes undergo a distinct series of observable phases as they move through this process transitioning from a stage of proliferation to one of hypertrophy and finally capillary invasion. Chondrocytes produce matrix vesicles (MVs) that they release into the growth plate and that attach to …

Live Cell Biomass Tracking For Basic, Translational, And Clinical Research, Graeme Murray

Theses and Dissertations

Single cell mass is tightly regulated throughout generations and the cell cycle, making it an important marker of cell health. Abnormal changes in cell size can be the first indication of dysfunction in response to environmental stimuli such as cytotoxic drugs. Described here is the further development of high-speed live cell interferometry (HSLCI) to concurrently measure the changes in single cell mass of thousands of cells over time. Critically, the high-throughput nature of HSLCI provides realistic pictures of tumor heterogeneity. This throughput enabled HSLCI to correctly predict in vivo carboplatin sensitivity of three triple negative breast cancer patient derived xenografts, …

Prolactin Drives A Dynamic Stat5a/Hdac6/Hmgn2 Cis-Regulatory Landscape Exploitable In Er+ Breast Cancer, Justin M. Craig

Theses and Dissertations

The hormone prolactin has been implicated in breast cancer pathogenesis and regulates chromatin engagement by the transcription factor, STAT5A. STAT5A is known to inducibly bind promoters and cis-regulatory elements genome-wide, though the mechanisms by which it exerts specificity and regulation of target gene expression remain enigmatic. We previously identified HDAC6 and HMGN2 as cofactors that facilitate prolactin induced, STAT5A mediated gene expression. Here, multi-condition STAT5A, HDAC6, and HMGN2 ChIP-seq with parallel condition RNA-seq are utilized to reveal the cis-regulatory landscape and cofactor dynamics underlying prolactin stimulated gene expression in breast cancer. We find that prolactin regulated genes are …

Bayesian Experimental Design For Bayesian Hierarchical Models With Differential Equations For Ecological Applications, Rebecca Atanga

Theses and Dissertations

Ecologists are interested in the composition of species in various ecosystems. Studying population dynamics can assist environmental managers in making better decisions for the environment. Traditionally, the sampling of species has been recorded on a regular time frequency. However, sampling can be an expensive process due to financial and physical constraints. In some cases the environments are threatening, and ecologists prefer to limit their time collecting data in the field. Rather than convenience sampling, a statistical approach is introduced to improve data collection methods for ecologists by studying the dynamics associated with populations of interest. Population models including the logistic …

First-Year Retention At Virginia Commonwealth University: Understanding Student Departure And The Potential Impact Of Academic Advising, Ben Plache

Theses and Dissertations

This dissertation examines first-year enrollment at Virginia Commonwealth University to determine what factors are associated with an increased likelihood of a student not being retained, and for which of these factors there is evidence that academic advising is an effective intervention. A survey of common retention models identified six factors associated with retention likelihood: student background prior to enrollment (“pre-enrollment factors”), financial support, institutional support, institutional performance, institutional engagement, and student intention. Prior research has shown that academic advising is an effective intervention for two of these factors: institutional performance and institutional support.

The significance of these factors was tested …

Amixicile, A Novel Antimicrobial Agent, And Its Effect On The Salivary And Subgingival Microbiome In Rhesus Macaque Monkeys, Erin E. Block

Theses and Dissertations

Background/Purpose: Periodontitis is an inflammatory disease with a bacterial etiology in a susceptible host. A selective antibiotic with minimal systemic side effects could be a useful adjunct to traditional periodontal therapy. Amixicile is a novel antimicrobial agent that targets pyruvate: ferredoxin oxidoreductase (PFOR), an enzyme that is critical for anaerobic bacterial metabolism and has been found to have little-to-no side-effects in animal models. The aim of this study was to prospectively evaluate the effect of amixicile on clinical periodontal parameters and the composition of the oral microbiome in rhesus macaque monkeys. It was hypothesized that the amixicile would reduce growth …

Antibiotic-Loaded Dendrimer Hydrogels In Periodontal Bone Regeneration: A Feasibility Study, Nicholas G. Yesbeck

Theses and Dissertations

Purpose: Prescription of a complete course of oral antibiotics following bone grafting procedures is a common clinical practice in surgical periodontics. Prophylactic administration of antibiotics reduces the risk of surgical site infection and systemic administration is preferred over local delivery because of short duration of action in the latter circumstance. The goal of this study is to demonstrate the ability of dendrimer hydrogels (DH) to prolong the release kinetics of antibiotics in-vitro. A secondary goal will be to analyze the effect of different particulate bone allografts on the release kinetics of the antibiotic.

Methods: Dendrimer hydrogels (DH) were synthesized …

Targeting Transferrin Binding Protein A For Vaccine Development In Neisseria Gonorrhoeae, Ashley Greenawalt

Theses and Dissertations

Neisseria gonorrhoeae, the causative agent of the sexually-transmitted infection gonorrhea, has recently been labeled a superbug. With dwindling treatment options, there is urgent need for a gonococcal vaccine. TonB-dependent transporters (TDTs) are important gonococcal virulence factors that allow the gonococcus to pirate metals directly from host proteins. The TDTs, TbpA and TbpB, are promising vaccine targets because both proteins are expressed and highly conserved in gonococcal strains, and they are not subject to high-frequency antigenic variation. Because the Tbps bind to host protein as their natural function, our immune system does not recognize them as foreign antigens; thus, immunogenicity is …

Socio-Ecological Predictors Of Social Connection Among Older Adults, Georganna Amateau

Theses and Dissertations

Decades of research has established an unequivocal link between states of social connection and health status. Lack of social connection, whether construed as social isolation or loneliness, negatively influences health and is highly associated with cardiovascular disease, high blood pressure, fall risk, and premature death. Despite extensive research on social isolation and loneliness, evidence relative to the broader construct of social connection suffers. Few studies inform practice standards for community-based organizations. This study aimed to develop a multidimensional, continuous composite variable of social connection and use the composite variable to examine predictors with a socio-ecological lens.

A secondary data analysis …

Systematic Structure-Activity Relationship Studies Of Nalfurafine, Mengchu Li

Theses and Dissertations

Today’s opioid crisis is one of the most challenging public health emergency. Two million Americans suffer opioid addiction. Over 20 million adults are affected by daily pain without non-addictive analgesics.

Kappa opioid receptor (KOR) is one of the most studied new pain targets void of abuse liability. KOR agonists have shown potent analgesia and also potential in alleviating opioid addiction. The only approved KOR agonist in clinic is nalfurafine (NFU). Though not leading to addiction, the analgesic effects of NFU are accompanied by sedative side effects. Therefore, we designed a systematical structure-activity relationship study of NFU for developing non-addictive analgesics. …

Ck2 Phosphorylation Of Human Papillomavirus 16 E2 On Serine 23 Promotes Interaction With Topbp1 And Is Critical For E2 Plasmid Retention Function, Apurva Tadimari Prabhakar

Theses and Dissertations

Human papillomaviruses (HPVs) are the causative agents in most cervical cancers and have been implicated in a rising number of oropharyngeal cancers. HPV positive oropharyngeal cancers have increased significantly over the last two decades with no specific anti-viral therapeutic options for the treatment of HPV diseases. During its lifecycle, HPV16 encoded E2 protein interacts with host cellular factors to regulate viral transcription, replication and genome segregation/retention. Our understanding of the cellular partner proteins that E2 uses to mediate its functions remains incomplete.

Previously, an interaction between E2 and the host protein TopBP1 was identified. In earlier studies, it was also …

Strategies For Resolution Enhancement In Liquid Chromatography, Shelby Lynn Weatherbee

Theses and Dissertations

Recent research in liquid chromatography (LC) has led to the development of new methodologies to further improve sample analysis. Non-uniform stationary phases are one such area that focuses on developments in selectivity and efficiency of stationary phases. Within this subset of stationary phases are two major focuses: two-dimensional liquid chromatography (2D-LC) and continuous stationary phase gradients that are the basis of this work.

In this work, LC simulation software was adapted to simulate chromatograms based on experimental and modeled injection profiles. This was done by measuring the injection profiles from a 2D-LC active solvent modulation valve using a variety of …

Detrimental Role Of Irf1 In Myeloid Cells During Influenza Infection, Bianca L. Baum

Theses and Dissertations

The influenza virus is constantly evolving new ways to overcome both innate and vaccine-induced host defenses, making the innate immune response a critical therapeutic target for minimizing morbidity and mortality caused by infection. Here we examined the role that interferon regulatory factor 1 (IRF1) plays in regulating host innate immune responses in myeloid cells. We found that knocking out IRF1 from myeloid cells in mice (IRF1^DMy) led to a partial protection during IAV infection. Interestingly, recruitment of bone marrow derived myeloid cells as well as NLRP3 inflammasome expression were decreased in the lungs of IRF1^DMy mice, whereas …

Il-36g Cytokine Induction In Oral Epithelial Cells Triggered By Treponema Denticola And Their Role In Chronic Periodontitis, Christopher Blake

Theses and Dissertations

Periodontal disease is a polymicrobial disease affecting 10-15% of the population. Periodontal disease affects the quality of life by reducing mastication and potentially causing tooth loss. Periodontal disease is also linked to more severe, systemic diseases such as cardiovascular disease and rheumatoid arthritis. Development of the diseased state occurs when the plaque biofilm shifts from healthy to dysbiotic. The shift to a dysbiotic biome brings with it a shift in bacterial presence, specifically an increase in gram-negative bacteria, such as T. denticola. T. denticola is a gram-negative, anaerobic spirochete whose relationship with oral epithelial cells is poorly understood. Preliminary data …

Canalpro Eal Accuracy Compared To The Root Zx Eal, Benjamin T. Brown

Theses and Dissertations

CanalPro Electronic Apex Locator Accuracy Compared to the Root ZX Electronic Apex locator

Benjamin Brown, Garry Myers, Caroline Carrico, Duane Schaefer

Department of Endodontics, Virginia Commonwealth University

Objectives: Electronic apex locators (EAL) are an effective instrument in measuring the working length of a canal. The Root ZX is considered the gold standard by many. The aim of this research is to compare the accuracy of the CanalPro EAL to the Root ZX.

Methods: The actual length of 43 single rooted extracted teeth were measured with a #10 hand file when the tip was visualized at the foramen under a microscope. …

Difficult Paintings, Hao (Damien) Ding

Theses and Dissertations

The sublime as a concept has a fraught and racist history. However, it remains the single most helpful idea in describing the deeply felt state of being when one comes across something ineffably powerful. From an art-making perspective, this thesis, and the accompanying exhibition of installations and paintings, proposes an alternative construction of the concept of the sublime. Using Lacanian psychoanalysis as a conceptual point of departure, a painter can manipulate the relationship of the viewer and paintings to create paradoxical moments of simultaneous intimacy and distance, which interact to create an alternative path towards the sublime. Through descriptions of …

Virtual Reality Tool For Visualizing The Oral Microbiome, Alexus M. Harris

Theses and Dissertations

There are limited teaching tools for helping both students and patients understand the oral microbiome. It is important to understand the oral microbiome as it heavily influences the health of your oral cavity and the dysbiosis of disease. The oral microbiome influences other aspects of health and serves as an indicator for health. With a greater understanding of the oral microbiome, patients can make healthier lifestyle changes and dentists can better educate their patients. For that reason, the goal of this project was to create a virtual reality tool for visualizing the oral microbiome. The assets for the VR tool …

Molecular Basis Of Cross-Sensitization In Colonic Inflammation-Induced Somatic Hypersensitivity, Parshva K. Mehta

Theses and Dissertations

A major portion of pain experienced by patients with an Irritable Bowel Disease (IBD) or Irritable Bowel Syndrome (IBS) can be attributed to visceral hypersensitivity. Visceral stimuli transmitted through primary afferent neurons in the dorsal root ganglia (DRG) induce a nociceptive response. Notably, a subset of patients has also experienced the development of somatic pain, such as leg pain, after diagnosis of a bowel disorder. The aim of this investigation is to ascertain which biochemical mediators are involved in the development of such viscerosomatic cross-sensitization. Initially, the Von Frey Test was used to find behavioral evidence of somatic referred pain; …

In Vitro Evaluation Of The Solubility Of Endosequence Bc Sealer In Acidic Solutions, Inderpal S. Sappal

Theses and Dissertations

Purpose: In recent years, bioceramic sealers (BCS), such as EndoSequence BC Sealer, have gained popularity in endodontic practices. Previous research has found BCS difficult to remove from canals during retreatments when compared with other endodontic sealers. Acidic solutions have been found to inhibit the set of BCS and increase their solubility. The purpose of this study was to evaluate the efficacy of acidic solutions in the removal of BCS as compared with a traditional retreatment solvent.

Methods: Roots of extracted human single rooted teeth (N=15) were prepared into 17 mm sections, then instrumented with ProTaper Gold (PTG) rotary files up …

The Effects Of Same-Sex Parental Relationships On Teenagers, Denise A. Daniels

Theses and Dissertations

Having same-sex parents is often linked with negative effects to the well-being of children into their adult years. The reported experiences from teenagers with same-sex parents and the stressors they experience are next to nonexistent. For this study, an interpretative phenomenological analysis was conducted to understand how teenagers age 13-16 make meaning of their same-sex parents’ relationship and the stressors they experience.

The theoretical framework for this study was based on the collaborative language system (CLS) of Anderson and Goolishian. The researcher investigated the perceptions of four teenaged participants regarding their relationships with their same-sex parents and how they coped …

The Impact Of Training Teachers In Multiple-Intelligences Instructional Strategies, Mary R. Massey

Theses and Dissertations

This research study outlined a professional-development program for training preschool teachers in Howard Gardner’s theory of multiple intelligences. The program then continued training in practical applications of the theory for instructional use in the classroom. The preschool director and teachers’ perceptions and experiences were documented and provided the data to assess the impact of the training on the instructional strategies demonstrated in the classrooms. Attention was paid to any impact on classroom management, teacher morale, and cohesive instructional vision throughout the school.

The data from this study revealed that the years of experience of the teachers proved to be the …

Gender Disparity In Studying Chemistry, Carlo Semerzier

Theses and Dissertations

This applied dissertation was designed to determine if there is a difference between students’ gender, ethnicity, and age and their performance in General Chemistry I at a Christian University in Florida. Many scientific studies reveal the existence of a gender performance gap in chemistry: women mostly underperform men. Certain factors reported by researchers and cited in this study that might contribute to this gap include self-efficacy, math ability, prior conceptual knowledge in chemistry, attitude toward chemistry, spatial ability, discrimination, learning styles, and exam types.

This quantitative research study used retrospective data from 113 students from eight sections (2016-2019) of a …

Teaching Strategies Leading To Success In Self-Contained Classrooms, Sonja Michelle Robinson-Madden

Theses and Dissertations

This applied dissertation addressed the strategies used in self-contained classrooms that lead to the success of students with intellectual disabilities in Jamaica. The study was prompted by the poor performance of primary schools in teaching delivery as evidenced in the National Inspectorate Report. The views of successful teachers were collated in order to provide meaningful research data that can be used to improve the effectiveness of teachers regarding their teaching strategies and engagement of their students in the teaching-learning process. In addition, the research findings may be used to inform policies regarding the education of special needs students who are …

A Qualitative Examination Of Student Threat Assessment In Nassau County, New York, Christopher J. Cleary

Theses and Dissertations

Law enforcement agencies and school systems face the critically important task of preventing attacks of targeted school violence and providing safe environments for students, faculty, and staff. The tragic results of past school shooting incidents have caused law enforcement agencies to work collaboratively with educators to develop strategies to reduce violent attacks.

Numerous examples from previous school shooting incidents show that shooters exhibited certain warning signs before the attack, and those signs went unaddressed. The student threat assessment process was developed to help educators and law enforcement members recognize the warning signs and take preemptive action before violence occurs. Threat …

Retaining Employees In Public Sector Organizations, Marlon Javier Rivera

Theses and Dissertations

This research study focused on the attrition rate among employees at a large public sector organization within a large state in the southeastern United States. The study identified factors contributing to the problem and identify possible solutions to minimize or alleviate the problem. The literature identified motivation as one of the factors largely contributing to increasing attrition rates.

Motivation has become a rather slow-paced factor in this large public sector organization and was reflected in work performance and productivity (Director, 2019). Cooperation between departments and individuals of higher positions tended to be deficient and communication was minimal (Director, personal communication, …

Community Attitudes Towards Juvenile Sex Offenders And Registration In Virginia, Paige Reed

Theses and Dissertations

The collateral consequences and harmful effects of juvenile sex offender registration and restrictions has been the forefront of conversation surrounding sex offender legislation in the United States. The literature available has consistently shown that juveniles profoundly differ from adults in significant areas such as decision making, cognitive capability, judgment, and overall development. The literature has also consistently shown that juvenile sex offenders are amenable to treatment, have considerably low recidivism rates, and are unlikely to offend into their adulthood. Despite this, and even though the juvenile justice system prioritizes rehabilitation over punitive measures, juvenile sex offenders are commonly subjected to …

Therapists' Perspectives On Aiding Individuals To Maintain Social Connection While Struggling With Health Concerns, Shari Howington-Carlin

Theses and Dissertations

This applied dissertation was designed to explore therapists’ perspectives on aiding individuals to maintain social connection while struggling with health concerns. Social relationships support physical and psychological well-being, yet individuals struggling with physical or mental health issues often have difficulty maintaining their relationships. Therapists, as health professionals, often discuss physical as well as emotional health concerns with their patients, including components of physiological and psychological heath care. Emerging neuroscience is aiding in understanding how research in this field supports early intervention for health outcomes around healthy social and family relationships during chronic disease or illness onset.The researcher conducted a phenomenological …

Exploring The Aftermath Of Cyber And Traditional Bullying Victimization Of Adolescent’S Mental Health And Academic Success, Latasha L. Young

Theses and Dissertations

The problem addressed in the research study was that cyber bullying and traditional bullying continued to increase at the research site during the past 4 years. The purpose of the study was to determine the influences of mental health services that were designed to decrease the negative effects of both cyber and traditional bullying on adolescent clients’ health and academic performance. This applied dissertation was designed to examine the experiences of cyber and traditional bullying victimization of adolescents’ mental health and academic success, within a large southern state in the United States, to provide practical solutions to address the concerns …