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From Origin To Application: A Study Of Plant Orphan Genes, Seth Jordan O'Conner
From Origin To Application: A Study Of Plant Orphan Genes, Seth Jordan O'Conner
Theses and Dissertations
As sequencing technology has taken off since the late 1990's, a unique phenomenon has been observed repeatedly: genes with little to no conservation across species. For a while, the predominant theory that arose to explain these genes was duplication and subsequent evolution of conserved genes. While this theory can explain some, still many genes have now been proven to arrive de novo - from previously non-coding DNA. This work further investigates the origins of these de novo evolved genes and their practical application relevance in crop biotechnology. This work demonstrates the dynamic nature of plant mitochondrial genomes between even closely …
Weedy Rice (Oryza Sativa Ssp.): An Untapped Genetic Resource For Abiotic Stress Tolerant Traits For Rice Improvement, Shandrea D. Stallworth
Weedy Rice (Oryza Sativa Ssp.): An Untapped Genetic Resource For Abiotic Stress Tolerant Traits For Rice Improvement, Shandrea D. Stallworth
Theses and Dissertations
Rice (Oryza sativa) is the staple food for more than 3.5 billion people worldwide. As the population continues to grow, rice yield will need to increase by 1% every year for the next 30 years to keep up with the growth. In the US, Arkansas accounts for more than 50% of rice production. Over the last 68 years, rice production has continued to grow in Mississippi, placing it in fourth place after Arkansas, Louisiana, and California. Due to increasing rice acreage, regionally and worldwide, the need to develop abiotic stress-tolerant rice has increased. Unfortunately, current rice breeding programs lack genetic …
The Effect Of Pain Tolerance Feedback On Human Aggression, Suzanne C. Amadi
The Effect Of Pain Tolerance Feedback On Human Aggression, Suzanne C. Amadi
Theses and Dissertations
Pain is a sensory experience associated with physical discomfort that is influenced by cognition and emotion and has been linked to an increased risk for aggression. The purpose of the current study was to examine the association between pain and aggression under controlled laboratory conditions using both experimental and non-experimental approaches. The aims of the study were two-fold. First, to manipulate perceived pain tolerance via faux feedback and then observe whether aggression differs as a function of this pain perception manipulation using a laboratory analogue of aggression. Second, to examine whether self-ratings of pain sensitivity and behavioral measures of pain …
Evaluation And Mechanisms Of Host-Plant Resistance To The Wireworm-Diabrotica-Systena Complex In Sweetpotatoes (Ipomoea Batatas) And A Commercial Kairomone Lure In Diabroticites, Thomas J. Douglas
Theses and Dissertations
An evaluation of host-plant resistance to the wireworm-Diabrotica-Systena (WDS) complex of root-feeding insects in sweetpotatoes was performed on a total of 15 cultivars and advanced lines over the course of 4 years (2017-2020). It was found that sweetpotatoes varieties can differ significantly in amount of damage incurred. Several mechanisms of resistance were proposed and tested: periderm toughness, dry weight percentage, and volatile organic chemical defenses. No significant difference was found amongst the varieties tested concerning the physical properties. Chemical analysis was limited in scope but did show some differences between a susceptible variety when compared to a resistant variety in …
Identification And Evaluation Of Mycotoxins Produced By Macrophomina Phaseolina, Vivek Hemant Khambhati
Identification And Evaluation Of Mycotoxins Produced By Macrophomina Phaseolina, Vivek Hemant Khambhati
Theses and Dissertations
The fungus Macrophomina phaseolina (Tassi) Goidanich (Mp) is the causal agent of charcoal rot in soybean and infects over 500 plant species worldwide. Mp produces various mycotoxins and is suspected of utilizing a toxin-mediated process to penetrate host tissue. Identification and evaluation of secondary metabolites produced by Mp will further elucidate the pathogenesis mechanisms used by the fungus. Mp cultures isolated from soybean were evaluated for phytotoxicity in a hydroponic soybean bioassay and chemically analyzed by LC-MS/MS. All Mp cultures at two dilutions induced phytotoxicity symptoms including chlorosis, necrosis, wilting, stunting, and death. Analysis identified 13 unreported secondary metabolites including …
Kinetic Spectroscopic Quantification Of Biomarkers In Practical Samples, Weiyu Peng
Kinetic Spectroscopic Quantification Of Biomarkers In Practical Samples, Weiyu Peng
Theses and Dissertations
Kinetic spectroscopic quantification refers to a subset of chromogenic (CG) and fluorogenic (FG) assays that deduce analyte concentration based on the UV-vis or fluorescence signal obtained during the CG/FG reaction processes. Existing kinetic spectroscopic quantification are based predominantly on reactions that can be approximated as a first-order process. Presented in this thesis is the kinetic spectroscopic quantification that uses higher order CG/FG reactions where the overall reaction can be approximated as combination of two sequential first-order processes. Included in chapter one is the theoretical model and several proof-of-concept applications. This model analyte is malondialdehyde (MDA), a lipid peroxidation biomarker of …
A Machine Learning Approach To Predicting Seafloor Properties And Their Application In Estimating A Global Methane Hydrate Inventory, Taylor Runyan Lee
A Machine Learning Approach To Predicting Seafloor Properties And Their Application In Estimating A Global Methane Hydrate Inventory, Taylor Runyan Lee
Theses and Dissertations
Seafloor properties, including total organic carbon (TOC) and the vertical thickness (isochores) of geological units, are sparsely measured on a global scale and spatial interpolation (prediction) techniques are often used as a proxy for observations. Previous geospatial interpolations of seafloor TOC exhibit gaps where little to no observed data exists. Recent machine learning techniques, based upon a suite of geophysical and geochemical properties (e.g., seafloor biomass, porosity, distance from coast) show promise in making globally complete, comprehensive, and statistically robust geospatial seafloor predictions. Here I apply a non-parametric (i.e., data-driven) machine learning (ML) algorithm, specifically k-nearest neighbors (kNN), to estimate …
Existence And Multiplicity Of Positive Solutions For One-Dimensional P-Laplacian With Nonlinear And Intergral Boundary Conditions, Xiao Wang
Theses and Dissertations
In this dissertation, we study the existence and multiplicity of positive solutions to classes of one-dimensional singular p-Laplacian problems with nonlinear and intergral boundary conditions when the reaction termis p-superlinear or p-sublinear at infinity. In the p-superlinear case, we prove the existence of a large positive solution when a parameter is small and if, in addition, the reaction term satisfies a concavity-like condition at the origin, the existence of two positive solutions for a certain range of the parameter. In the p-sublinear case, we establish the existence of a large positive solution when a parameter is large. We also investigate …
Temporal Alterations In Bovine Placental Capacity During Compromised Pregnancies, Zully E. Contreras
Temporal Alterations In Bovine Placental Capacity During Compromised Pregnancies, Zully E. Contreras
Theses and Dissertations
The circadian rhythms are not solely regulated by photoperiod but are also influenced by feed regimen. Therefore, maternal nutrient restriction during gestation could potentially impact the fetal circadian rhythm. Melatonin, a circadian rhythm modulator hormone, has shown to act as an antioxidant reducing reactive oxygen species during stress exposure; and two potential mechanisms have been proposed for melatonin causing vasoconstriction and vasodilation regulating blood flow. In livestock species, nutrient restriction during gestation reduces uterine blood flow, limiting nutrients availability to the fetus for growth and development. Therefore, this project aimed to use beef heifers to evaluate the maternal nutrient restriction …
Penstemon's Centromeric Histone 3 Variation And The Impact Of Soil Steaming On High Tunnel Tomato Production, Xin Ye
Theses and Dissertations
We evaluated the genetic diversity of Penstemon's Centromeric Histone 3 (CENH3), which localizes to chromosome centromeres in eukaryotes. From RNA extractions of 56 species, we successfully amplified two CENH3s (termed CENH3a and CENH3b), which share approximately 69% sequence homology across the length of the gene and about 85% across the histone fold domain (HFD). CENH3a is generally 72 bp longer than CENH3b and contains seven exons while CENH3b is made of five. Unlike studies of CENH3 in other plants, Penstemon's CENH3 N-tail was found to be highly conserved, indicative that the genus has undergone a short evolutionary history. Surprisingly, of …
Evaluating The Influence Of Ecosystem Characteristics And Species Traits On Exotic Species Distributions, Adrián Lázaro-Lobo
Evaluating The Influence Of Ecosystem Characteristics And Species Traits On Exotic Species Distributions, Adrián Lázaro-Lobo
Theses and Dissertations
Natural dispersal mechanisms and biogeographical barriers have shaped species' native distributional ranges over millions of years. However, over the last few centuries, humans have dispersed species beyond their natural ranges. Those species that undergo explosive population growth and rapid expansion in the introduced region are considered as invasive because they have the potential to cause negative effects on desirable species and/or ecosystem services. In chapter II, I identified what ecosystem characteristics are more closely associated with successful establishment of exotic and native species, to have a better idea of where to concentrate our efforts and resources to prevent invasion events …
The Nose Glows: Investigating Amphibian Neuroendocrine Pathways With Quantum Dots, Allison Rebecca Julien
The Nose Glows: Investigating Amphibian Neuroendocrine Pathways With Quantum Dots, Allison Rebecca Julien
Theses and Dissertations
Today, amphibian extinction rates are rising at an alarming rate. Captive assurance colonies have become a hedge against extinction, and often must employ assisted reproductive technologies (ART) in species that do not readily breed in captivity. One technique that can be utilized in assisted breeding is hormone therapy, which involves the treatment of individuals with exogenous reproductive hormones. The primary delivery method used in most breeding programs is intraperitoneal injection, but many institutions either lack the training necessary to conduct this invasive procedure, or require veterinary staff to perform them, thus delaying breeding events. Therefore, there is interest in alternate …
Determining The Optimal Dile:Dlys Ratio For Ross 708 X Ross Yp Male Broilers Throughout Multiple Grow Out Phases (Starter, Grower, And Finisher), Andrew Taylor Brown
Determining The Optimal Dile:Dlys Ratio For Ross 708 X Ross Yp Male Broilers Throughout Multiple Grow Out Phases (Starter, Grower, And Finisher), Andrew Taylor Brown
Theses and Dissertations
Within commercial broiler production, feed and feed manufacturing costs account for 60 to 70% of production costs. Of these feed costs specifically, ingredients that supply energy and protein represent the highest expenditure, verifying the importance of knowing the nutrient requirements of current broiler strains to ensure accurate least-cost formulation. To reduce these production costs and optimize performance, primary breeder companies and nutritionists continuously strive to improve nutrient utilization of commercial broiler strains. As a result of this, a great magnitude of work is conducted to determine the nutrient requirements for broilers, especially amino acids. Therefore, the overall objective of this …
The Effects Of Developmental Chlorpyrifos Exposure On The Proteome Of The Adolescent Rat Hippocampus, Aubrey Lewis
The Effects Of Developmental Chlorpyrifos Exposure On The Proteome Of The Adolescent Rat Hippocampus, Aubrey Lewis
Theses and Dissertations
Chlorpyrifos is a widely used organophosphate insecticide, functioning through the inhibition of acetylcholinesterase. Recent studies report negative long-lasting biochemical and behavioral effects at levels without acetylcholinesterase inhibition. Our lab studies have identified the endocannabinoid system as a target for OP low-dose neurotoxicity. This thesis identifies the proteins and their associated neurotransmitter systems in the hippocampus that have been affected by low dose developmental exposure to the OP insecticide CPF. Male rat pups were treated from postnatal day 10 (PND) - PND16 with either corn oil (vehicle), 0.75 mg/kg of CPF, or 0.02 mg/kg of PF-04457845, a specific fatty acid amide …
Modeling Recombination Rate As A Quantitative Trait Reveals New Insight Into Selection In Humans, Austin L. Drury
Modeling Recombination Rate As A Quantitative Trait Reveals New Insight Into Selection In Humans, Austin L. Drury
Theses and Dissertations
Meiotic recombination is both a fundamental biological process required for proper chromosomal segregation during meiosis and a fundamental genomic parameter that shapes major features of the genomic landscape. While there is strong evidence of fitness costs of low rates of recombination, the possible fitness costs of high rates of recombination are less defined. To determine whether a single lower fitness bound can explain the variation in recombination rate observed in human populations, we simulated the evolution of recombination rate as a quantitative trait using empirically-derived parameters. For our fitness function, we implemented a hyperbolic tangent curve with flexible parameters to …
Effects Of The In Ovo Injection And Dietary Supplementation Of L-Ascorbic Acid On The Performance, Tissue L-Ascorbic Acid Concentrations, Inflammatory Response, And Trachea Histomorphology Of Ross 708 Broilers Raised Under Normal And Elevated Atmospheric Ammonia Levels, Ayoub Mousstaaid
Theses and Dissertations
Effects of various levels of L-ascorbic acid (L-AA) (12 and 25 mg) in ovo injected at 17 and 18 days of incubation, on embryonic and posthatch physiological variables in Ross 708 broilers were investigated. There were no significant treatment effects on the hatchability or serum concentrations of L-AA in the broilers. Eye L-AA concentrations were higher, and plasma nitric oxide levels were lower in male chicks treated in ovo with 12 mg of L-AA. While exposed to elevated atmospheric NH3 levels, the in ovo injection of 12 mg of L-AA increased the body weight gain and decreased the feed conversion …
Effects Of Year-Round Supplemental Feeding Of White-Tailed Deer On Plant Community Dynamics, Beau Navarre
Effects Of Year-Round Supplemental Feeding Of White-Tailed Deer On Plant Community Dynamics, Beau Navarre
Theses and Dissertations
Supplemental feeding is commonly practiced to enhance available nutrition for white-tailed deer (Odocoileus virginianus). The effects of supplemental feeding on the surrounding vegetative community may be related to herbivory, trampling, and seed dispersal. I evaluated how these potential mechanisms affect vegetative communities using a matched-pair design (fed and ecologically equivalent unfed sites) during 2018-2020. In a short-term manipulative portion of the study, I sampled the vegetation prior to feeding and during two years of feeding. In a long-term retrospective study, I sampled feeders established 5-7 years previously. Feeders increased daily detection rate of deer and seed dispersing non-target wildlife, percentage …
Effects Of Acute, Chronic, And Cyclical Hypoxia On The Physiology And Transcriptome Of Channel Catfish (Ictalurus Punctatus), Brian Ott
Theses and Dissertations
Channel catfish (Ictalurus punctatus) are raised for aquaculture in ponds characterized by dramatic swings in dissolved oxygen concentration. If morning dissolved oxygen concentration falls below approximately 3.0 mg/L catfish consume less feed, leading to a reduction in growth and production. Although the effects of low oxygen on channel catfish appetite have been described, the underlying mechanisms responsible for those effects are unknown. Increased production of the neuropeptides corticotropin-releasing factor (CRF) and urotensin I (UI) are implicated in other fishes as neuropeptides that reduce appetite once an environmental stressor is detected. This project characterizes the hematological responses and transcriptional response of …
Oak Regeneration: Impacts Of Prescribed Fire And Invasive Species, Caleb Desmond Goldsmith
Oak Regeneration: Impacts Of Prescribed Fire And Invasive Species, Caleb Desmond Goldsmith
Theses and Dissertations
Across the central and eastern United States, upland oaks are experiencing regeneration complications. This thesis comprises two studies evaluating challenges in regenerating upland oaks. One study evaluates upland Quercus spp., Carya spp., and Acer rubrum L. response to prescribed fire and impacts on seedling growth and mortality. This study found prescribed fire promoted oak growth when canopy cover was < 77%. Oaks displayed lower percent mortality and higher resprout capacity after fire compared to red maple. The second study investigates impacts of Microstegium vimineum presence on Quercus alba L., Liriodendron tulipifera L., and Acer rubrum L. seedlings under different light and moisture environments. Microstegium vimineum presence decreased growth of both sweetgum and red maple; white oak was less impacted, likely because of its conservative growth strategy and high-water use efficiency. Oaks’ conservative growth strategy may offer more resiliency compared to its competitors in response to prescribed fire and Microstegium vimineum.
Regeneration Potential And Habitat Suitability Modeling Of Three Imperiled Southeastern U.S. Woody Plants, Clayton Warren Hale
Regeneration Potential And Habitat Suitability Modeling Of Three Imperiled Southeastern U.S. Woody Plants, Clayton Warren Hale
Theses and Dissertations
Presented within this thesis are three studies on three rare and imperiled Southeastern woody plant species: mountain stewartia (Stewartia ovata), Atlantic white-cedar (Chamaecyparis thyoides), and Miller's witch-alder (Fothergilla milleri). This work contributes to the ecological understanding of these three species allowing for better-informed conservation decision-making. Machine learning habitat suitability models are presented for mountain stewartia and Miller's witch-alder. These models can direct limited conservation dollars and manpower towards areas of the highest habitat suitability. This work also utilizes field-based data to assess the habitat needs, species associations, and regeneration potential of both Atlantic white-cedar and Miller's witch-alder. Understanding the habitat …
Commercial Douglas Fir Biochar Based Multifunctional Exotic Adsorbents For Water Remediation, Chanaka Navarathna
Commercial Douglas Fir Biochar Based Multifunctional Exotic Adsorbents For Water Remediation, Chanaka Navarathna
Theses and Dissertations
Providing safe drinking water and wastewater remediation are constant worldwide challenges. Adsorption is an attractive alternative to conventional techniques such as coagulation, precipitation (chemically or electrochemically), hybrid membranes, and ion-exchange for the purification of water. Biochar-based composite sorbents are increasingly popular because a range of surface chemical and physical treatments can impart performance and environmental benefits to the material. This is ideal for rural areas where more costly conventional methods may not be readily available or affordable. This dissertation focused on three different projects involving high surface area (~700 m2/g) Douglas fir biochar based multifunctional engineered adsorbents. Chapter II focuses …
Effects Of Reduced Predator Abundance On The Predator-Prey Community Of A Tropical Reservoir, Cynthia Holt
Effects Of Reduced Predator Abundance On The Predator-Prey Community Of A Tropical Reservoir, Cynthia Holt
Theses and Dissertations
In most Puerto Rico reservoirs, Largemouth Bass Micropterus salmoides are typically fast-growing with distributions composed of distinct year-classes. Cerrillos Reservoir, however, displays the classic characteristics of a crowded bass population common in temperate lakes and ponds with fish stockpiling into a single modal distribution of slow-growing fish. This developed shortly after stocking in 1997, with the population quickly expanding to carrying capacity. Consequently, relative weight declined from above 100 to about 80 and the population was mostly composed of fish <=350 mm. A protected slot limit (356-508 mm) was implemented; however, limited effort and angler attitudes towards harvest of small bass prevented the success of this regulation, which was subsequently removed. In this dissertation, I examined the efficacy of using targeted management harvests for improving growth, size structure, and condition of Largemouth Bass in Cerrillos Reservoir. I used bioenergetics modeling to simulate the effects of targeted harvests. I predicted annual consumption of stock and quality-sized Largemouth Bass and used these estimates to model the effects of population reduction on consumption and growth of remaining bass. To validate the model, experimental removals of Largemouth Bass from the crowded size classes (200-380 mm) were conducted in 2012 and 2013. In these two years, I removed 20% and 22% of the total biomass, respectively. Following the experimental removals, mean condition of Largemouth Bass increased from 83 to 90, and increases were observed in all Largemouth Bass size categories. The reduction in predator abundance resulted in increased prey availability and smaller size structure of sunfish (Lepomis spp.) and tilapia (Coptodon and Oreochromis spp.) populations. I concluded that reducing Largemouth Bass abundance in Cerrillos Reservoir can move population dynamics toward desired management outcomes, but these actions must continue indefinitely to achieve success and sustainability. Annual management harvests are feasible but impractical, so I recommend increasing angler harvest and implementing management efforts designed to limit recruitment (e.g., shoreline rotenone application) to achieve management goals.
Puma And The Innate Immune Response During Pneumococcal Infection In The Lung, Daniel Edward Kennedy Ii
Puma And The Innate Immune Response During Pneumococcal Infection In The Lung, Daniel Edward Kennedy Ii
Theses and Dissertations
Background: The p53-up-regulated modulator of apoptosis (PUMA) protein is a pro-apoptotic, BH3-only member of the BCL2 family of effector proteins responsible for promoting organized cell death. PUMA is required for resolution of pneumococcal pneumonia in mice, as mice deficient of PUMA exhibit greater numbers of S. pneumoniae CFU within tissues and higher mortality rates than observed in Puma+/+ mice. Methods: Puma+/+ and Puma-/- mice were intranasally challenged with TIGR4 pneumococcus and sacrificed 24 h post-infection. Differences in cytokine levels from blood and whole lung tissue were detected by MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel. Lung transcriptomes from Puma+/+ and …
Consequences Of External Factors On Placental And Offspring Development And Using Melatonin As A Potential Therapeutic, Dana S. Reid
Consequences Of External Factors On Placental And Offspring Development And Using Melatonin As A Potential Therapeutic, Dana S. Reid
Theses and Dissertations
Early life is critical for the development of an organism. External factors alter placental efficiency which can lead to consequential effects. The objective of the current study was to (1) examine placental characteristics and molecular factors affected by nutrient restriction (2) evaluate the mitigating properties of melatonin in a nutrient restricted pregnancy in regard to circadian, myogenic and adipogenic factors in fetal muscle and (3) evaluate the effects of prenatal and postnatal melatonin supplementation on offspring performance. In study 1, cows were fed a control (CON) or a 60% restricted (RES) diet from day 140 to 240 of gestation. Animals …
Phenotypic And Genotypic Characterization And Comparison Of Edwardsiella Ictaluri Isolates Derived From Catfish And Ornamental Fish Species, Divya Divya
Theses and Dissertations
The gram-negative bacteria Edwardsiella ictaluri causes significant economic losses in aquacultured fish. Generally considered host-specific to catfish, there are reports of E. ictaluri outbreaks from other aquacultured species, including ornamental fish raised in the southeastern U.S. Thus, a comprehensive phenotypic and genotypic characterization of E. ictaluri isolates from catfish and ornamental aquaculture was warranted. Morphological, biochemical, and protein profiles of catfish and ornamental derived isolates were mostly similar. Plasmid profiles of wild-type isolates were consistent within groups. Analysis of putative anti-microbial resistant isolates from catfish revealed the presence of multi-drug resistant plasmids. Genomic comparisons indicated marked differences among host groups, …
Odor In The Global Environmental Context: The Effect Of Odor Context Reinstatement On Memory, Dustin D. Finch
Odor In The Global Environmental Context: The Effect Of Odor Context Reinstatement On Memory, Dustin D. Finch
Theses and Dissertations
Previous research has demonstrated that memory is dependent on the environmental context; memory is better when the same environmental context cues present at study are reinstated at test as compared to when they are not. This finding is called context reinstatement effects (Godden & Baddeley, 1975; Smith, Glenberg, and Bjork, 1978; Smith & Vela, 2001). What is unclear is whether study items are associated with the global context or with unique features within the study environment. We tested whether reinstating a singular feature of a global environment, the odor present during study, was sufficient to produce context reinstatement effects. These …
Oxidative Stress Biomarkers In Blood Plasma Of Moderately Exercised Horses, Elizabeth Catesby Ott
Oxidative Stress Biomarkers In Blood Plasma Of Moderately Exercised Horses, Elizabeth Catesby Ott
Theses and Dissertations
Equine athletes are subjected to environmental and physical stressors resulting in oxidative stress that can negatively impact performance. Oxidative stress can result in lipid peroxidation, cell damage, and DNA degradation leading to physiological dysfunction and increased instance of disease. It has been established that humans are able to adapt to oxidative stress when exposed to extended periods of high-intensity exercise, however, this has yet to be established in the equine model. In the present study, we sought to establish patterns of oxidative stress expression immediately following exercise and adaption to prolonged exposure to exercise training in the equine model. Results …
Pain And Inflammation Due To Whole-Body Vibration In A Rat Model, Folly Martha Dzan Patterson
Pain And Inflammation Due To Whole-Body Vibration In A Rat Model, Folly Martha Dzan Patterson
Theses and Dissertations
Low back pain is a leading cause of disability and is associated with whole-body vibration exposure in industrial workers and military personnel. The pathophysiological mechanisms by which whole-body vibration causes low back pain have been studied in vivo, but there is little data that improve diagnosis of low back pain. The overall objective of this research was to elucidate diagnostic biomarkers associated with whole-body vibration. Hence, a rat model for vibration-induced inflammatory responses was developed. Von Frey filaments were used to determine the withdrawal threshold of the hind paw as a surrogate behavioral marker for pain. The concentration of nerve …
Mucinomics: A Bioinformatic Analysis Of Snail Mucins, And Their Function, Maxwell B. Mcdermott
Mucinomics: A Bioinformatic Analysis Of Snail Mucins, And Their Function, Maxwell B. Mcdermott
Theses and Dissertations
This thesis outlines the current research on secreted snail mucus, highlighting the potential of this biopolymer, and also demonstrates a research strategy to fulfill the unmet need of examining the hierarchical structures that lead to the enormous biological and chemical diversity of snail mucus genes.
Protein-Based Nanofibers And Thin Films For Drug Delivery Applications, Christopher R. Gough
Protein-Based Nanofibers And Thin Films For Drug Delivery Applications, Christopher R. Gough
Theses and Dissertations
Corn zein and silk are both widely available, easy to extract proteins making them valuable replacements for synthetics. Zein and silk are also promising biomaterials in several applications. This work explores the use of both proteins as drug delivery vehicles by loading 1-Dimensional micro-nanofibers and 2-Dimensional thin films with model drugs or the topical therapeutic sodium citrate. Using a formic acid solvent, powdered zein protein or silk fibers were dissolved into solution and then casted into 2D films or spun into 1D fibers through air-spraying. During dissolution, therapeutic products are added. SEM images showed that fibers maintain their small diameter …