Digital Commons Network^™

Hybrid Suspension Tissue Engineering Of A 3d Alveolar Model For Lung And Vascular Disease Modeling, Jonard Corpuz Valdoz

Theses and Dissertations

Tissue engineering is a dichotomy of scaffold-based and scaffold-free cultures. Scaffold-based cultures form highly organotypic structures but with low uniformity and throughput. Conversely, scaffold-free cultures create consistently sized and shaped cell aggregates with limited spheroid-like structure and function, thus restricting their use for accurate disease modeling. We hypothesized that combining aspects of each culturing format, we would produce highly organotypic structures of consistent size and shape for use in pulmonary modeling. First, to improve on culture consistency and output, we created a novel easily scalable, minimalistic design for a micropatterned hydrogel dish that increases reliability and efficiency in 3D cell …

Enhancing The Opportunities For Adults With Autism To Find Jobs Using A Job-Matching Algorithm, Joseph T. Bills

Theses and Dissertations

Adults with autism face many difficulties when finding employment, such as struggling with interviews and needing accommodating environments for sensory issues. However, autistic adults also have unique skills to contribute to the workplace that companies have recently started to seek after, such as close attention to detail and trustworthiness. To work around these difficulties and help companies find the talent they are looking for we have developed a job-matching system. Our system is based around the stable matching of the Gale-Shapley algorithm to match autistic adults with employers after estimating how both adults with autism and employers would rank the …

Photophysics And Electronic Properties Of Photochromic Metal-Organic Frameworks, Corey R. Martin

Theses and Dissertations

Modulation of materials properties through light-based illumination has the capability to expand the technology sector due to stimuli-responsive dynamic behavior that cannot be achieved in traditional materials. For example, reversibly tuning photophysical profiles of such materials allows for switching between discrete states that is a key aspect for the developing logic gates, spatially- and temporally-resolved sensors, and on-demand drug delivery systems. My efforts have focused on employment of metal-organic frameworks (MOFs) as a versatile platform for the material development which contain photochromic moieties allowing for tailoring their electronic properties. Our group has expanded this direction to include heterometallic and actinide-containing …

Concurrent Identification, Characterization, And Reconstruction Of Protein Structure And Mixed-Mode Dynamics From Rdc Data Using Redcraft, Hanin Rafiq Omar

Theses and Dissertations

A complete understanding of the structure-function relationship of proteins requires an analysis of their dynamic behaviors and the static structure. However, all current approaches to studying dynamics in proteins have their shortcomings. A conceptually attractive and alternative approach simultaneously characterizes a protein's structure and its intrinsic dynamics⁠. Ideally, such an approach could solely rely on RDC data-carrying both structural and dynamical information. The major bottleneck in utilizing RDC data in recent years has been attributed to a lack of RDC analysis tools capable of extracting the pertinent information embedded within this complex data source.

Here we present a comprehensive strategy …

Cryptic Cryptophytes: The Plasticity In How Cryptophytes Respond To Changes In Resource Availability, Rachel Ann-Marie Schomaker

Theses and Dissertations

Resource variability and availability often drives competition within ecosystems, which can lead to the diversification of organismal niches and physiological capabilities. In aquatic systems, common resources that photosynthetic organisms, such as algae, compete for are light and carbon. Both the spectral characteristics (color) and carbon concentrations of an aquatic system vary with time and space, which means that algae need to be able to respond to changes in resource availability to survive. Using a group of ubiquitous unicellular eukaryotes known as cryptophytes, we investigated both how cryptophytes respond to changes in the available light spectrum at a physiological and genetic …

Exploration Of The Role Of Neighborhood Residential Segregation By Race And Ethnicity In Obesity Risk Among School-Aged, Melissa L. Fair

Theses and Dissertations

Youth obesity prevalence remains high, despite decades of intervention. Grounded in the social ecological model, neighborhoods and schools are important settings in addressing the complex systems that influence obesity. Contributing to disparities, by race/ethnicity, Black and Hispanic youth are more likely to live in segregated neighborhoods and attend segregated schools, which are also more likely to have high rates of poverty and are less likely to have high quality nutrition and built environments. Leveraging data from local school district (district and state department of education) and national datasets (ACS, Childhood Opportunity Index 2.0), this study examined the associations between school …

Effectiveness Of An Ecowellness Intervention For College Aged Individuals With A Developmental Disability, Olivia Jean Lewis

Theses and Dissertations

Nature has the ability to heal many psychological wounds from anxiety and depression, stress, post traumatic stress disorder, and behavioral concerns. As a result, clinicians are recognizing the power of nature as a tool in the healing process. The purpose of this dissertation, which consists of one completed systematic review and a multiple probe single case study is to (a) provide background information on the current literature regarding nature, (b) create a manualized EcoWellness treatment intervention, and (c) test the effectiveness of the treatment intervention in order to provide practicing counselors with a potential new intervention to increase psychological wellbeing …

Results On Select Combinatorial Problems With An Extremal Nature, Stephen Smith

Theses and Dissertations

This dissertation is split into three sections, each containing new results on a particular combinatorial problem. In the first section, we consider the set of 3-connected quadrangulations on n vertices and the set of 5-connected triangulations on n vertices. In each case, we find the minimum Wiener index of any graph in the given class, and identify graphs that obtain this minimum value. Moreover, we prove that these graphs are unique up to isomorphism.

In the second section, we work with structures emerging from the biological sciences called tanglegrams. In particular, our work pertains to an invariant of tanglegrams called …

Discovery And Mechanistic Study Of Novel Pbd-Targeted Inhibitors Of Plk1 For Prostate Cancer Treatment, Danda Pani Chapagai

Theses and Dissertations

Polo-like kinase 1 (PLK1) is a central player in regulating entry into and progression through mitosis. Inhibition of sub-cellular localization and kinase activity of PLK1 through the Polo-box domain (PBD) is emerging as a viable alternative to ATP binding site directed drugs for which the development of resistant mutants and inhibition of closely related members of the PLK family (tumor suppressor roles) are primary concerns. I describe related novel non-peptidic PBD binding inhibitors, termed abbapolins, identified through successful application of the REPLACE strategy and demonstrate their potent antiproliferative activity in prostate tumors and other cell lines. Furthermore, the abbapolins show …

Exploring Genetic Counselors’ Experiences, Language, And Discussion Of Consanguinity In Clinical Practice: A Multinational Perspective, Romy Isabel Fawaz

Theses and Dissertations

Consanguinity, defined as the degree of relationship between closely related individuals, is a widespread historical practice that is not specific to any one religion, population, or region of the world. Genetic counselors regularly ask whether a reproductive couple is consanguineous as part of the review of family history and risk assessment. Couples who are in consanguineous relationship may be subject to negative attitudes and stigma, potentially due to cultural differences and norms at a population level and these attitudes may interplay with questions, answers, and discussions around consanguinity. We hypothesized that genetic counselors may experience some level of discomfort discussing …

Psychophysiological Biomarkers Of Concussion Recovery, Adam Todd Harrison

Theses and Dissertations

Each ach year a growing number of individuals report lingering deficits months-years following concussion. Persistent post-concussion symptoms (PPCS) can negatively impact day-today activities and if left untreated may manifest in severe neurological sequelae resulting in long-term cognitive impairment or advanced neurological degeneration (i.e., CTE). Current clinical diagnostic and prognostic assessments (e.g., symptom reports and neurocognitive testing) lack the sensitivity to quantify neurological function. Accordingly, there is a critical need to identify objective biomarkers specific to PPCS to improve an individual’s quality of life and prevent severe long-term neurological dysfunction.

Psychophysiological measurements (e.g., EEG derived event-related potentials, heart rate variability, and …

Perspectives About Food Safety In Diverse Low- And Middle-Income Countries, Sejla Isanovic

Theses and Dissertations

Concerns about unsafe food influence food choice, and consumption of unsafe foods increases morbidity and mortality, particularly in low- and middle-income countries. Actions to ensure safety of food are dominated by mitigation of biological and chemical hazards through supply-side risk management, disregarding individuals’ experiences and perspectives of food safety. We aimed to identify and categorize perspectives about food safety in five countries. Five Drivers of Food Choice projects provided transcripts from 17 focus groups discussions and 303 interviews in Kenya, Ghana, India, Guinea, and Vietnam. We analyzed transcripts using a priori and emergent codes. Individuals constructed meaning about food safety …

Inheritance And Appropriation: Confronting Privilege In Magical Young Adult Fiction, Natalie Jipson

Theses and Dissertations

This thesis investigates the way that magical ability and the lack thereof has been racialized through the deployment of blood-based models of inheritance in young adult fantasy literature. Because of the connection between blood and biology, texts that only allow magical transmission from parent to child place significant limits on who can access witchery, creating systems of privilege that enforce racial and classed hierarchies. I will use magical novels written by Laurie Forest, Graci Kim, Nnedi Okorafor, Amy Rose Capetta, and Daniel José Older to illustrate the hierarchies created by blood-based models, as well as to demonstrate the possibilities that …

Mindset, Stereotype Threat, And The Fear Of Failure: Why Female Students Leave A Secondary High Honors Mathematics Pathway, Annabelle Merg

Theses and Dissertations

The disproportionate attrition of female students in STEM is well documented in higher education but less well understood at the secondary level. In a large, public high school in an affluent, highly educated city, the high honors mathematics pathway is the only math pathway that has not yet achieved gender parity, an implication for future educational and economic equity. Female students leave this pathway at significantly higher rates than male students, especially during and immediately after the first course. Through an explanatory, mixed methods approach, this study found that these female students are not less well prepared than their male …

Understanding The Genomic Influence And Virulence Capabilities Of Environmentally Isolated Vibrios, Shannon Elizabeth Pipes

Theses and Dissertations

The genus Vibrio consists of Gram-negative bacteria that possess a curved rod shape and are routinely isolated from estuarine and coastal salt water. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the major three species that contribute to human disease worldwide, and a variety of other Vibrio species contribute to persistent problems in the aquaculture and fishing industries. The CDC estimates that vibrios cause 80,000 cases of disease each year in the United States alone, however, this number is thought to be underestimated, since some disease is only self-limiting, meaning patients may not seek medical treatment and have …

Tangled Up In Tanglegrams, Drew Joseph Scalzo

Theses and Dissertations

Tanglegrams are graphs consisting of two rooted binary plane trees with the same number of leaves and a perfect matching between the two leaf sets. A Tanglegram drawing is a special way of drawing a Tanglegram; and a Tanglegram is called planar if it has a drawing such that the matching edges do not cross. In this thesis, we will discuss various results related to the construction and planarity of Tanglegrams, as well as demonstrate how to construct all the Tanglegrams of size 4 by looking at two types of rooted binary trees - Caterpillar and Complete Binary Trees. After …

From Gene Expression To Physiology: A Study Of Chronic Thermal Tolerance In The Mytilus Edulis Species Complex, Lindsey Cate Schwartz

Theses and Dissertations

Rising ocean temperatures are a severe and ever-present threat to marine life. With environmental temperature having such a large impact on organismal performance, understanding the mechanisms which contribute to the ability to survive at higher temperatures is a crucial research focus. Significant progress has been made in discovering these mechanisms on the cellular, biochemical, and physiological levels, but is it much less common for those to be examined together. Using the Mytilus edulisspecies complex as a model system, this dissertation takes a closer look at how prolonged exposure to sub-lethal high temperatures impacts marine organisms on multiple levels of …

“Describing Without Identifying”: The Phenomenological Role Of Gender In Cataloging Practices, Travis L. Wagner

Theses and Dissertations

This dissertation explores gendering practices of visual information catalogers. The work aims to understand how catalogers perceive gender when describing persons within visual information. The qualitative study deployed queer interpretative phenomenological analysis to understand how catalogers think broadly about describing identity. The infused queer theoretical tenets helped to understand that while participants may not directly name gender as challenging, the conflation of gender into cisnormative monoliths (assuming every person's gender matches their sex-assigned-at birth) or silence around gender produce telling opinions concerning nonbinary gender. The research also utilized a Think Aloud exercise wherein participants undertook in-the-moment cataloging three moving images. …

S-Equol: A Novel Therapeutic For Hiv-1 Induced Gut Dysbiosis, Mason T. Rodriguez

Theses and Dissertations

HIV-1 infection affects approximately 38 million people around the world. The advent of cART has greatly improved the quality of life of infected individuals; however, roughly 50% of these individuals will still experience HIV-1 associated neurocognitive disorders (HAND). Additionally, the gut microbiome has been reported to be dysbiotic in HIV-1 infected individuals, regardless of adherence to cART. Current research has pointed to the gut-brain-microbiota axis as a potential target to treat both cognitive deficits and microbial changes. The present study investigated S-equol (SE) as a potential therapeutic for HAND by modulating the gut microbiome. The study included 21 HIV-1 Tg …

The Impact Of Cannabis And Alcohol Use On The Management Of Type 1 Diabetes Mellitus, Joshua Eric Sellner

Theses and Dissertations

This dissertation sought to contribute to the evidence-base of diabetes management and acute complications associated with alcohol and cannabis use among individuals with Type 1 Diabetes (T1DM). Three interconnected studies were initiated to complete this goal. First, existing literature surrounding the potential consequences of cannabis consumption among individuals with T1DM were summarized within a systematic review. Study results were combined to provide a single measure of association through a meta-analysis. Second, identified associations were further investigated through a cross-sectional study using the SEARCH for Diabetes in Youth data on alcohol and cannabis use on diabetes management and acute complications including …

Associations Of The Fto Gene And Risk Of Acute Myeloid Leukemia, Avery Ulrich

Theses and Dissertations

Background Acute Myeloid Leukemia (AML) is a blood cancer that affects ~70,000 people each year in the US. The Fat Mass and Obesity (FTO) gene on chromosome 16 has been reported to be associated with obesity, solid tumor cancers (lung, renal, breast, prostate, pancreatic, endometrial), and more recently with hematologic malignancies, like AML. In this thesis, we test the hypothesis that inherited variants within the FTO gene are associated with an increased risk of AML. Methods Using the DISCOVeRY-BMT (Determining the Influence of Susceptibility COnveying Variants Related to one-Year mortality after Blood and Marrow Transplantation), a well-powered genome-wide association study …

Growing Up Water, Dominique Barbee

Theses and Dissertations

“Growing Up Water” is a full-length manuscript consisting of one long poem in two parts. Louisiana is one of the most verdant naturally fertile states in the U.S. The state loses a football field of land almost every one-hundred minutes. This is largely due to climate change, oil drilling in the gulf, the cutting of canals, the waste poured in to the Mississippi river by the chemical plants that line this area (known as “Cancer Alley”), the onslaught of invasive species, the list goes on. Furthermore, due to global warming, “big” hurricanes are only becoming stronger and more frequent. Any …

The Effect Of Explicit Vocabulary Instruction On Elementary Students’ Vocabulary Knowledge And Reading Comprehension: An Action Research Study, Tonia Bauer

Theses and Dissertations

Reading comprehension seems to be a significant challenge for rising fifth graders with limited vocabulary knowledge. A possible solution is to provide them with explicit vocabulary instruction which may positively impact their reading comprehension. This action research aimed to evaluate the effect of implementing explicit vocabulary instruction delivered through Schoology on fifth graders’ vocabulary knowledge and reading comprehension at an urban elementary school in the southeastern United States. This study also sought to analyze students’ perceptions of the usefulness of the online vocabulary learning modules. A convergent mixed-method approach was applied to answer the research questions. The quantitative data (e.g., …

Deep Learning Based Generative Materials Design, Yong Zhao

Theses and Dissertations

Discovery of novel functional materials is playing an increasingly important role in many key industries such as lithium batteries for electric vehicles and cell phones. However experimental tinkering of existing materials or Density Functional Theory (DFT) based screening of known crystal structures, two of the major current materials design approaches, are both severely constrained by the limited scale (around 250,000 in ICSD database) and diversity of existing materials and the lack of a sufficient number of materials with annotated properties. How to generate a large number of physically feasible, stable, and synthesizable crystal materials and build accurate property prediction models …

High School Students’ Math And Science Motivation Profiles: Stability And Relationship With Their Stem Career Aspirations, Jiali Zheng

Theses and Dissertations

Motivation to study mathematics and science is an important influencing factor of career aspirations in STEM fields which predicts STEM major choice in college and STEM careers after graduation. Using restricted data from a nationally representative sample HSLS:09, the current study identified U.S. high school students’ motivation profiles in mathematics and science courses in 9^th and 11^th grade, examined the stability of these profiles across the two time points, and studied the association between 11^th grade motivation profiles and STEM career aspirations. Differences between male and female students in motivation profiles, profile stability and career aspirations were …

Mediator Kinase Inhibitors For Breast Cancer Therapy, Xiaokai Ding

Theses and Dissertations

Breast cancers (BrCa) that overexpress oncogenic tyrosine kinase receptor HER2 are treated with HER2-targeting antibodies (such as trastuzumab) or small-molecule kinase inhibitors (such as lapatinib). However, most patients with metastatic HER2+ BrCa have intrinsic resistance and nearly all eventually become resistant to HER2-targeting therapy. Resistance to HER2-targeting drugs frequently involves transcriptional reprogramming associated with constitutive activation of different signaling pathways. We have investigated the role of CDK8/19 Mediator kinase, a regulator of transcriptional reprogramming, in the response of HER2+ BrCa to HER2-targeting drugs. Selective CDK8/19 inhibitors (senexin B and SNX631) showed synergistic interactions with lapatinib and trastuzumab in a panel …

Comparative Assessment Of The Fate And Toxicity Of Chemically And Biologically Synthesized Silver Nanoparticles Toward Juvenile Clams, Mercenaria Mercenaria, Amar Yasser Al-Rshim

Theses and Dissertations

Engineered nanomaterials (ENMs) with unique nanoscale properties, including novel optical behavior and superparamagnetic, are continually being developed for biomedical and industrial applications. In specific biomedical applications, ENMs are surface-functionalized using polymers, proteins, and other stabilizing agents to facilitate their resistance to salt-induced aggregation. Since their colloidal stability in high ionic-strength matrices, functionalized ENMs are anticipated to be persistent aquatic contaminants. Despite their potential environmental significance, the persistence of surface-functionalized ENMs as individually stabilized nanoparticles in marine environments is largely unknown. Further, few studies have investigated the fundamental factors that influence ENMs uptake and fate/transport processes in ecologically susceptible aquatic biota, …

Evaluation Of The Perceived Benefit Of A Psychiatric Resource For Parents Of Children With 22q11.2 Deletion Syndrome, Kayla Blankenship

Theses and Dissertations

The objective of this study was to assess the perceived utility of an educational resource booklet addressing the psychiatric manifestations and mental health considerations within 22q11.2 deletion syndrome (22q11.2DS).

Seventy-three parents of children with 22q11.2DS completed online surveys before and after reading the booklet. The surveys assessed personality factors and feelings of empowerment, worry, self-stigma, and ability to tolerate uncertainty.

Participants reported that the booklet was easy to understand, provided better understanding of 22q11.2DS and mental illness, answered questions about mental illness associated with the condition, improved knowledge of strategies for protecting the mental health of children with the condition, …

The Effects Of The Online Remediation Of Phonological Processing Deficits On Functional Reading Abilities In Students With Dyslexia, Fletcher Bowden

Theses and Dissertations

Dyslexia affects between 5% and 18% of Americans and is caused by difficulty with phonological processing. This study investigates the impact of an online intervention designed to remediate phonological processing deficits on reading accuracy, fluency, and comprehension. It also investigates changes to student self-concept and parent perceptions of their children’s reading attitudes and abilities as a result of the online intervention.

Ten students participated in the intervention; assessments were administered at the beginning and at the midpoint of the treatment. Scores in Phonological Processing and Alternate Phonological Processing, as measured by the CTOPP-2, demonstrated large to very large effect sizes, …

The Relation Between Adhd Symptoms And Alcohol And Marijuana Use Outcomes Among College Students: The Mediating Role Of Experiential Avoidance, William Joshua Bradley

Theses and Dissertations

Individuals with attention-deficit/hyperactivity disorder (ADHD) are at significantly greater risk for alcohol and marijuana misuse and abuse compared to their peers. College students with ADHD may be particularly vulnerable to problematic alcohol and marijuana use, given the widespread use and abuse of these substances by college students generally and liability associated with ADHD specifically. Experiential avoidance, or avoidance of unwanted thoughts, feelings, and other internal experiences, is positively associated with ADHD symptoms and substance use problems. However, it is unclear what role experiential avoidance plays in the relation between ADHD and alcohol and marijuana use. This study examined whether experiential …