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Bovine Viral Diarrhea Virus Infections Affect Professional Antigen Presentation In Bovine Monocytes, Sang-Ryul Lee

Theses and Dissertations

Monocytes are professional antigen presenting cells (APC). They serve as precursors of macrophages and dendritic cells (DC). We have used cytopathic (cp) and non-cytopathic (ncp) Bovine Viral Diarrhea Viruses (BVDV) to determine the genes and proteins expression levels in bovine monocytes. Four specific aims were accomplished in this study. The first aim was to assess the baseline expression of the proteins involved in professional antigen presentation in bovine monocytes. The results showed that the differential detergent fractionation (DDF) approach can provide interpretable and meaningful functional information in bovine monocytes. The second aim was to evaluate the role of in vitro …

Fuzzy Logic Techniques Applied To A Switchgrass Ecological Site Suitability Model For The State Of Mississippi, Michele Louise Maxson

Theses and Dissertations

Fuzzy logic provides a natural way of dealing with models that require the modeler or user to interpret linguistic variables in order to make a decision. This study focuses on employing fuzzy logic methodologies in order to create an Alamo switchgrass ecological site suitability map for the state of Mississippi. The inputs in the model are available water content (AWC), slope, pH, drainage, color, and texture. All variables were examined through the fist six inches (15cm) of soil. These six variables were assigned fuzzy restrictions based on expert knowledge from plant and soil scientists, combined using the fuzzy additive weighting …

Physiologically-Based Toxicokinetic And Toxicodynamic (Pbtk/Td) Modeling Of A Ternary Organophosphorus Insecticide Mixture In Rats: Model Development And Validation, Julian Thomas Pittman

Theses and Dissertations

A physiologically-based toxicokinetic and toxicodynamic (PBTK/TD) model was developed, from the open literature, to predict the toxicokinetic disposition and toxicodynamic response (acetylcholinesterase inhibition) of a ternary organophosphorus (OP) insecticide mixture: chlorpyrifos (CP), methyl parathion (MP) and parathion (P). In vivo studies were conducted in adult male Sprague-Dawley rats, orally administered one of two CP/MP/P mixtures (2.5, 0.5, 0.5 mg/kg or 5, 1, 1 mg/kg) with selected tissues (blood, brain, diaphragm, liver, lung and skeletal muscle) collected at 30min, 4, 12 and 24hr postdosing. Low dosages were studied so the mixture did not result in significant disruption of cardiovascular function nor …

Postsecondary Transitions Of Mississippi Band Of Choctaw Indians Tribal Scholarship Program Students, Gregory A. Carlyle

Theses and Dissertations

The purpose of this study was to explore the postsecondary education transition experiences of graduates of Choctaw Central High School (CCHS) who received support from the Tribal Scholarship Program (TSP) to better understand the barriers to successful postsecondary completion and the means to overcoming those barriers. Successful transitions are key for students to persist to academic completion. Studies show that American Indian students have low rates of college completion and experience a variety of factors attributed to withdrawal from college. This study provided insight into college success experienced by American Indian students and an understanding of the opportunities for a …

Stink Bugs On Grain Sorghum In Mississippi: Ecological Relationships With Host Plants And Crop Planting Dates And Crop Damage, Mario Ernesto Parada Jaco

Theses and Dissertations

This study was conducted to determine infestation levels of southern green stink bug, Nezara viridula (L.), and green stink bug, Acrosternum hilare (Say), on host plants including corn, cotton, soybean, sorghum, and non-crop vegetation in a multicrop ecosystem. Stink bug infestations responsible for crop damage on vegetative and panicle stages of grain sorghum planted on different dates were also investigated. Both N. viridula and A. hilare appeared to establish a first generation on non-crop grasses surrounding cultivated fields and then moved to soybean or cotton to begin a second generation. These stink bugs did not appear to be attracted to …

Subcanopy Response To Variable-Density Thinning In Second Growth Forests Of The Pacific Northwest, Emily Julia Comfort

Theses and Dissertations

Variable-density thinning (VDT) is a management option designed to increase structural heterogeneity in second-growth conifer stands. This study examined subcanopy tree growth response to two variations of VDT. At the Forest Ecosystem Study in western Washington, thinning intensity was found to have a significant effect on height growth of Douglasir (Pseudotsuga menziesii (Mirb.) Franco) seedlings which established following the thinning. At the Olympic Habitat Development Study in western Washington, basal area growth response was examined for residual midcanopy western hemlock (Tsuga heterophylla (Raf.) Sarg.) and western redcedar (Thuja plicata ex. D. Don). Both species retained the ability to respond to …

Proteomic Analysis Of Listeria Monocytogenes, Sana Mujahid

Theses and Dissertations

Listeria monocytogenes is a deadly, Gram-positive foodborne pathogen that is ubiquitous in the environment. The bacterium expresses a number of virulence and stress adaptation proteins that support its pathogenic capabilities. Two-dimensional gel electrophoresis (2-DE) was used to map L. monocytogenes surface proteins, which play a central role in virulence, and to examine protein expression by L. monocytogenes grown on ready-to-eat meat, an important source of Listeria infections. A novel method for solubilization of surface proteins from L. monocytogenes for 2-DE was developed. Additionally, the unique proteome expressed by L. monocytogenes grown on a meat matrix was uncovered. The developed solubilization …

Purification And Structural Analysis Of Newcastle Disease Virus V Protein And Flowering Locus T (Ft) Protein, Swapna Jayapalan

Theses and Dissertations

Newcastle disease virus (NDV) is one of the paramyxovirus that has been studied at length since this virus infects the birds of all species. NDV is highly virulent in chickens and results in a high mortality rate because the V protein of NDV is found to inhibit the avian immune response system. No drugs are available for treating NDV therefore, determining the structure of V protein would help in developing a drug that can inactivate the V protein, thereby increasing the host immune response against viral infection. The research here is focused on purification and initial structural analysis of the …

Quantitative Genetic Analysis For Flowering Time In Primitive Upland Cotton, Gossypium Hirsutum L., And Chromosome Assignment Of Bac-Derived Ssr Markers, Yufang Guo

Theses and Dissertations

Cotton is a very important economical crop in the U.S. and throughout the world. The developments in molecular biology offer new and innovative approaches toward evaluating and understanding genetic mechanisms of important agronomical traits. Bacterial artificial chromosome (BAC) libraries have rapidly become the preferred choice for physical mapping. BAC-derived microsatellite or simple sequence repeats (SSRs) markers facilitate the integration of physical and genetic recombination maps. The first objective in this research was to identify chromosome locations of a set of BAC-derived SSR markers in tetraploid cotton. A total of 192 SSR primer pairs were derived from BAC clones of an …

Influence Of Habitat Associations And Distance From Low-Order Streams On Amphibian And Reptile Communities On Public Lands In East-Central Mississippi, Sarah Louise Gallagher

Theses and Dissertations

This project evaluated the influence of habitat parameters and distance from low-order streams on herpetofauna communities, in addition to evaluating the efficacy of 2 sampling methods. Amphibians were associated with mature hardwood forests with high density of large, highly decayed woody debris. Reptiles were associated with mixed pine-hardwood forests, woody debris, and vertical canopy structure. Overall, woody debris was an important habitat feature for amphibians and reptiles. Amphibians were detected in greater abundance and species richness near the stream. Reptiles were similar in abundance and species richness as distance increased from low-order streams. I recommend a SMZ width ¡Ý50 m …

The Effects Of Hurricane Katrina On The Structure, Performance, Capacity, And Future Of The Lumber Industry, Thomas Eric Mcconnell

Theses and Dissertations

This investigation focuses on the effects of hurricane Katrina on the structure, performance, capacity, and future of the lumber industry in the impacted area. The basis of the examination is a survey of both hardwood and softwood lumber mills in Mississippi, Louisiana, and Alabama. Total response rate was 42.4%. Results support the hypothesis that the storm did have a significant and negative effect on the commerce of responding facilities. Contribuing factors, such as raw materials supply and procurement, physical damage tothe mill and resulting variations to operation, productivity and quality issues, as well as human resources, were impacted in differing …

The Impact Of A Large Automotive Manufacturing Plant On Demographic Expansion And Educational Changes In A Mississippi School District, Ronnie Lynn Mcgehee

Theses and Dissertations

This study was designed to examine one school district’s response to the largest automobile plant in North America being built in its county. An economic development package worth in excess of one billion dollars would surely change the landscape of the community and the district for years to come, but how should the district respond? Nissan North America announced in November of 2000 that it would build the largest automobile plant in North America in Canton, Mississippi. This plant would employee over four thousand employees and thirteen suppliers to that plant that would employee another five to six thousand employees …

Movement And Habitat Use Of Selected Nongame Fishes In A Minnesota Lake, Michael David Habrat

Theses and Dissertations

Aquatic vegetation provides important habitats for fish, but these habitats are increasingly being altered anthropogenically. My research evaluated the movement and habitat use of three small rare fish species, the blackchin shiner Notropis heterodon (BCS), blacknose shiner Notropis heterolepis (BNS), and banded killifish Fundulus diaphanous, in a Minnesota lake. BCS and BNS traveled farther in spring than summer, but selection of habitat based upon macrophyte biovolume did not explain these differences. All three fish species traveled long distances (> 1,800 m) and were capable of reaching all available habitats in Square Lake. Macrophyte species richness and prey (zooplankton) abundance were …

Traction-Induced Injury Of Rat Achilles' Tendon: New In Vivo Biomechanical Model For The Assessment Of Tendon Disease And Injury, Edward Brown Silverman

Theses and Dissertations

The goal of this project is to advance the understanding of tendon disease and injury through the design and validation of a new model of tendon injury. Controlled, mechanically-induced traction injury was created in rat Achilles’ tendons with a tensioning apparatus through a minimally invasive surgical approach. Tendons were tensioned to subailure loads. Assessments of damage to tendons included gross morphology, evaluation of biomechanical of load strain curves, histopathology under light microscopy (H&E), collagen fibril structure and disruption under scanning electron microscopy, and immunohistochemical study of MMP-13 expression under confocal laser microscopy. Tendon injuries were found to be more severe …

Study Of The Reproducibility Of Proteomics Methods And Variability Of Fruit Fly Proteomes., Thomas Franklin Culwell

Theses and Dissertations

The reliability of biomarker discovery by means of proteomics has been called into question. It was speculated that "background noise" variation resulting from differences in preparation and handling of samples and proteome dynamics may mask subtle, yet important, differences due to the biological condition. Little is understood about complex proteomes and their variability. A critical aspect of proteomic biomarker research that is largely unexplored is the comparative reproducibility of certain methods such as two-dimensional gel electrophoresis and liquid chromatography/mass spectrometry. In particular, with liquid chromatography/mass spectrometry, it is not known whether variability in peptide quantitation is dependent on any of …

Thermodynamic Evidence That Ganglioside-Mediated Insertion Of Botulinum A Into The Cholinergic Nerve Ending May Precede Endocytosis And Acidification: A Langmuir Film Study, Bradley Adam Strongin

Theses and Dissertations

Botulinum Neurotoxin (BoNT) is one of the most potent toxins known to human kind. The Atomic Force Microscope (AFM) was employed to investigate the conditions under which BoNT type A heavy chain would bind and/or insert into mica supported dipalmitoylphosphatidylcholine (DPPC) lipid bilayers. As an alternate technique, DPPC/GT1b or total ganglioside extract (80:20) monolayers of a Langmuir Blodgett (LB) Trough were adapted to be artificial membrane models for toxin insertion studies. We conclude that LB monolayer studies are a promising candidate for BoNT/A membrane insertion investigation. Botulinum neurotoxin serotype A insertions into the LB monolayers in the presence of BoNT/A …

Pyridinium Bis-Retinoids A2-Dopamine And A2-Cadaverine: Implications In Age-Related Macular Degeneration And Cancer, Mckenzie Ruth Pew

Theses and Dissertations

Age-related macular degeneration (AMD) is the leading cause of blindness in the United States of America. The pyridinium bis-retinoid A2-ethanolamine (A2E) has been implicated to play a role in AMD. We have observed novel pyridinium bis-retinoids through melanolipofuscin and human RPE extractions that may also play a role in the pathology of AMD. We have begun the construction of an amino-retinoid library in order to identify these ocular compounds. The compounds from the amino-retinoid library are also used in a targeted and triggered drug delivery system for treating cancer. Folic acid is coupled with the amino-retinoids to specifically target cancer …

The Role Of Inspiration In Scientific Scholarship And Discovery: Views Of Theistic Scientists, Kari Ann O'Grady

Theses and Dissertations

This qualitative research study examined the ways those who identify themselves as theistic scientists and scholars experience inspiration, as defined as divine guidance or influence, in their scientific scholarship and discovery. It also explored participants' beliefs about how scientists and scholars can seek and prepare to receive inspiration in their work. Open-ended surveys of 450 participants from the behavioral and natural sciences and from a variety of religious backgrounds were analyzed for content themes in the areas of experiences with inspiration, preparing to receive inspiration, and further thoughts on inspiration in science. The themes extracted indicated that these scientists and …

Constructive Enabling: Applying A Wilderness Skills Intervention To Support The Therapeutic Change Process Of Adolescent Females In Residential Treatment, Brian K. Malcarne

Theses and Dissertations

The purpose of this study was to explore the potential benefits of a primitive wilderness skills intervention (POST) on the therapeutic change process of adolescent females enrolled in a residential treatment center. A qualitative data analysis approach was used. A convenience sample of eight female adolescents was selected by therapist referral. The data were analyzed using open, axial, and selective coding. Data analysis was used to assess participants' progress in the therapeutic change. Further analysis revealed a core theme connecting participants' POST experience with feelings of self-empowerment and reflective connections to their individual therapeutic change process. Constructive enabling was the …

Effects Of Endurance Training On The Ampk Response To Exercise., David Gerald Chesser

Theses and Dissertations

Activation of AMP-activated protein kinase (AMPK) results in the upregulation of several intracellular systems which help to prepare a cell for a high energy challenge. The magnitude of the AMPK response to a 10 min bout of exercise has been found to decrease in red quadriceps (RQ) following training, while putative AMPK roles seem to be maintained; specifically, the biogenesis of mitochondria and higher levels of hexokinase II and glucose transporter 4 (GLUT4). If the AMPK response to exercise is responsible in part for these adaptations, how can they be maintained if the AMPK response is attenuated? The purpose of …

The Effect Of Febrile Temperature On Plasmodium Falciparum, Heidi Sue Porter

Theses and Dissertations

Previously it has been shown that cultures of Plasmodium falciparum died following exposure to a febrile temperature of 40°C, as demonstrated by a decrease in parasitemia of the following generation. In the current study, the effect of 40°C treatment on culture media, erythrocytes, and parasite glucose consumption, were ruled out as possible influences on parasite death, demonstrating that 40°C impacted the parasites directly. Metabolic profiling of DNA synthesis, protein synthesis, and glucose utilization during exposure to 40°C clearly indicated that febrile temperatures had direct effect on major metabolic pathways and parasite development, beginning 20-24 hr after erythrocyte invasion. The ring …

Women's Perceptions Of Parents, Peers, Romantic Partner And God As Predictive Of Symptoms Severity Among Women In Treatment For Eating Disorders At An Inpatient Facility, Samuel B. Tobler

Theses and Dissertations

The present study examined whether perceptions of parents, peers, romantic partners and God were predictive of eating disorder symptom severity among women in treatment for eating disorders. The sample included 417 women (ages 12 to 56 years) at an inpatient treatment facility for eating disorders. Participants completed a battery of assessment measures at intake and discharge. Change scores were also computed on all measures. Measures included indices of eating disorder symptomology, parental relationships, peer relationships, romantic partner relationships, and religious well-being. Multiple regression analysis showed perceptions of peers and romantic partner to be significant predictors in all analyses; however perceptions …

Family Leisure Involvement And Family Functioning In Samoa, Irene Dora Annandale Fotu

Theses and Dissertations

The purpose of this study was to (a) examine the relationship between family leisure involvement and aspects of family functioning (adaptability and cohesion) among Samoan families residing on American Samoa, and (b) to compare the Samoan data to a broad sample of American families to provide a cross-cultural comparison. The sample consisted of 340 adult participants. The Family Leisure Activity Profile (FLAP) was used to measure family leisure involvement. FACES II was used to measure family functioning. Regression analyses conducted on the 340 individuals indicated a positive relationship between core family leisure involvement and family cohesion, adaptability, and overall family …

Calcium Dynamics Affecting Egg Production, Skeletal Integrity, And Egg Coloration In Ring-Necked Pheasants (Phasianus Colchicus), Landon R. Jones

Theses and Dissertations

These 4 chapters represent manuscripts formatted for submission to journals based on an experiment conducted on Ring-necked Pheasants (Phasianus colchicus). Calcium limits the distribution of this species, which produces 7-15 eggs per clutch. They may renest up to 5 times per breeding season on a diet low in calcium. The first chapter examines egg production in laying hens on 7 different diets from 0.19-4.47% calcium in the absence of calcium loading. Calcium-loaded pheasants store calcium in medullary bone before an experiment and can draw on this surplus during egg production, possibly skewing experimental results. We measured egg production, egg characteristics, …

A Study Of Habitat Variables Associated With Northern Goshawk Nest Site Activity On The Three National Forests In Southern Utah, Keeli Shea Marvel

Theses and Dissertations

The Northern Goshawk has been a species of concern since its decline in the early 1990s, which has been attributed in part to loss of critical breeding and wintering habitat. Nest site selection of goshawks has been correlated with certain specific site characteristics including, but not limited to, forest species composition, forest stand size, diameter of nest tree, percent cover, tree height, site slope, and aspect. The goshawk holds the status of a Management Indicator Species (MIS) on all of the six national forests in Utah. This status requires annual monitoring to track goshawk numbers and to address any activities …

Phylogeography Of The Livebearer Xenophallus Umbratilis (Teleostei: Poeciliidae): Glacial Cycles And Sea Level Change Predict Diversification Of A Freshwater Tropical Fish, Carissa Poole Jones

Theses and Dissertations

The biogeography of Central America is considered a classical case study in understanding the impact of vicariant events on patterns of biotic dispersal. While many biogeographers have focused on community composition and geographic limits of species at broad scales across Central America, much less work has focused on post-colonization diversification patterns at finer scales. The livebearing freshwater fish Xenophallus umbratilis presents an ideal system for determining the impact of recent earth history events on biodiversity in northern Costa Rica. Here, we test the hypothesis that marine inundation of the San Carlos and Northern Limón basins during the Miocene has caused …

The Role Of Secreted Phosphoprotein-24 In Osteoblast Differentiation And Matrix Mineralization, Samuel Ramage

Theses and Dissertations

Secreted Phosphoprotein-24 (Spp24) was initially isolated and characterized as a component of bovine cortical bone matrix. Subsequent characterization has shown it is multiply phosphorylated and homologous to cystatin and TGF-β receptor type II. Spp24 is a minor component of the serum fetuin mineral complex that binds calcium-phosphate minerals and prevents their deposition. The TGF-β receptor homology domain binds BMP-2 weakly in vitro and enhances BMP-2’s osteogenic effects in vivo. The ability of Spp24 to affect BMP activity suggests an important role for Spp24 as a native, bioactive componentof bone that regulates bone development.

Spp24 was highly up-regulated in rat cortical …

Niche Separation Along Environmental Gradients As A Mechanism To Promote The Coexistence Of Native And Invasive Species, Edmund R. Priddis

Theses and Dissertations

Niche separation may be the key to promoting the long-term coexistence of introduced and native species. Physical alterations to the environment (habitat manipulation) or re-introducing native species to former habitats can exploit the maladapted traits of introduced species to create a refuge for native species. No two species have identical niches because evolutionary constraints differ between species with different evolutionary histories. Our objectives were to determine if cold temperatures could promote coexistence between native least chub and introduced western mosquitofish. We used individual scale and population scale experiments to test four hypotheses: 1) colder temperatures would reduce the aggressive behavior …

Effects Of Water Vapor On The Kinetics Of The Methylperoxy Radical Self-Reaction And Reaction With Hydroperoxy, Alecia Mower

Theses and Dissertations

The gas phase reactions of CH3O2 + CH3O2, HO2 + HO2, and CH3O2 + HO2 in the presence of water vapor have been studied at temperatures between 263 and 303 K using laser flash photolysis coupled with UV time-resolved absorption detection at 220 nm and 260 nm. Water vapor concentration was quantified using tunable diode laser spectroscopy operating in the mid-IR. The HO2 self-reaction rate constant is significantly enhanced by water vapor, consistent with what others have reported, whereas CH3O2 self-reaction and the cross-reaction (CH3O2 + HO2) rate constants are nearly unaffected. The enhancement in the HO2 self-reaction rate coefficient …

Neuronal And Molecular Adaptations Of Gaba Neurons In The Ventral Tegmental Area To Chronic Alcohol, Kimberly Hales

Theses and Dissertations

The purpose of this thesis project was to examine the effects of chronic alcohol on the excitability and molecular adaptation of GABA neurons of the ventral tegmental area (VTA). GABA neurons are of interest with regards to ethanol intoxication, reinforcement, and dependence due to their widespread distribution and connectivity to mesocorticolimbic dopamine (DA) neurons implicated in alcohol reward and addiction. Since we have previously shown adaptation of VTA GABA neuron firing rate to chronic ethanol (Gallegos, Criado et al. 1999) and suppression of gap-junction (GJ) mediated coupling between these neurons by acute ethanol (Stobbs, Ohran et al. 2004), we wanted …