Digital Commons Network^™

Tolerance And Accumulation Of Copper, Lead And Zinc By Ceratopteris Richardii, Dorothy Jelagat Cheruiyot

Theses and Dissertations

Environmental contamination has increased rapidly in recent years. There is a need to develop cheap and environmentally friendly remediation methods. Numerous studies have evaluated the ability plants to remove contaminants from the environment. The present study evaluated the ability of Ceratopteris richardii gametophytes and sporophytes to tolerate copper, lead and zinc and for the sporophytes to accumulate the three metals in their shoots. Spores were sown in plant medium treated with varying concentrations of Cu, Pb and Zn. Fifteen-day- old sporophytes were transferred into medium mixed with varying concentrations of the three metals. Ceratopteris richardii tolerated and accumulated each of …

Assessment And Accountability: An Exploration Of Teachers' Practices In Assessing English Language Proficiency, Mari B. Rasmussen

Theses and Dissertations

Assessment is an important component in an education program. Current educational policy includes large scale assessments with stringent accountability requirements. All students are expected to meet standards for achievement, including those who have historically not been successful.

Students from non-English language backgrounds are one of the populations of students who do not achieve academic success at the same rate as other students. This population of students is increasing in our nation's schools, causing this achievement gap to be of greater concern. Added to the concern is the requirement that English Language Learners (ELLs) must meet rigorous standards in both academic …

Agenda-Setting And The Media: A Look At Child Welfare Legislation, 1995-2005, Tamara Temoney

Theses and Dissertations

This project used a content analysis methodology to determine the agenda-setting capacity of the media to influence child welfare legislation in a state legislature. With a foundation in agenda-setting theory, this study identifies how the coverage of child abuse and neglect in the print media impacts decision-makers to introduce legislation related to child abuse and neglect. Through a comparison of the issues covered in the newspaper with the issues receiving legislative attention, this study showed that media influence varies by topic, with some topics being more open to media affects than others. A second component of this study analyzed how …

Creation Of A Mouse With A Humanized Fpgs Gene Compatible With Normal Development, Linying Xie

Theses and Dissertations

Abstract: Folylpoly-γ-glutamate synthetase (FPGS) catalyzes the formation of polyglutamate forms of the reduced folates and antifolates such as methotrexate (MTX) and pemetrexed; this allows the retention of folates and antifolate cancer drugs inside the cell. The enzyme activity of FPGS is essential for cell proliferation and survival. The mouse fpgs gene contains two promoters spaced 10 kb apart which are activated in a tissue-specific manner. The upstream promoter (P1) and exons A1a and A1b are used in some differentiated tissues, mainly liver and kidney, whereas the downstream promoter (P2) and exon 1 are used in rapidly dividing cells. In contrast, …

A Meta-Analysis Of Adolescent Rampage Shootings And The Potential Interconnectivity To Gifted/Talented Children With Emotional/Behavioral Disorders, Meghan M. Salyers

Theses and Dissertations

On the tenth anniversary of a series of school rampage shootings in Springfield, Oregon, Littleton, Colorado, and Conyers, Georgia, the purpose of this study was to determine whether there were indications of intellectual, academic, or creative giftedness with emotional/behavioral disorders in the perpetrators. In addition, through historiographical methods, this study was designed to allow for themes to surface from the authentic writings of the adolescent shooters.

Similarities in previous research results are reported in the meta-analysis, and categories derived from my analysis of the writings of Kip Kinkel, Eric Harris, Dylan Klebold, and T.J. Solomon are indicated in the analysis …

Real-Time Automatic Price Prediction For Ebay Online Trading, Ilya Igorevitch Raykhel

Theses and Dissertations

While Machine Learning is one of the most popular research areas in Computer Science, there are still only a few deployed applications intended for use by the general public. We have developed an exemplary application that can be directly applied to eBay trading. Our system predicts how much an item would sell for on eBay based on that item's attributes. We ran our experiments on the eBay laptop category, with prior trades used as training data. The system implements a feature-weighted k-Nearest Neighbor algorithm, using genetic algorithms to determine feature weights. Our results demonstrate an average prediction error of 16%; …

Design And Testing Of A Pumpless Microelectromechanical System Nanoinjector, Quentin Theodore Aten

Theses and Dissertations

A deeper understanding of human development and disease is made possible partly through the study of genetically modified model organisms, such as the common mouse (Mus musculus). By genetically modifying such model organisms, scientists can activate, deactivate, or highlight particular characteristics. A genetically modified animal is generated by adding exogenous (foreign) genetic material to one or more embryonic cells at their earliest stages of development. Frequently, this exogenous genetic material consists of specially engineered DNA, which is introduced into a fertilized egg cell (zygote). When successfully introduced into the zygote, the exogenous DNA will be incorporated into the …

The Promise Of Academic Learning Time In A Dose-Response Model Of Early Reading Achievement, Benjamin Heuston

Theses and Dissertations

Reading has long been acknowledged to be a critical skill that is best acquired early in life. According to the most recent National Assessment of Educational Progress (NAEP) reports, American public school children continue to struggle to master the basics of reading. Although federal funding in real dollars has increased consistently over time, reading scores have not followed suit, suggesting that fiscal resources have not been applied successfully to the variables that are directly related to reading acquisition and achievement. The current state of affairs therefore suggests the need for identifying a fiscally-targetable, instructionally-relevant variable with a direct, causal relationship …

Synthesis And Characterization Of Novel Temperature-Responsive Dendritic Peg-Pdlla Star Polymers For Drug Delivery, Arunvel Kailasan

Theses and Dissertations

This study describes a novel thermoresponsive dendritic polyethylene glycol-poly(D, L-lactide) (PEG-PDLLA) core-shell nanoparticle with potential for drug delivery and controlled release. A series of dendritic PEG-PDLLA nanoparticles were synthesized through conjugation of PEG to Starburst™ polyamidoamine (PAMAM) dendrimer G3.0 and subsequent ring-opening polymerization of DLLA, in which PEG chain length (i.e., MW=1500, 6000 or 12000 Dalton) was varied; however, the feeding molar ratio of DLLA monomers to the overall PEG repeat units on the dendrimer surface was kept at 1:1. Linear PEG-PDLLA copolymers were also syntheiszed under the same condition and used as control. According to our results, dendritic PEG-PDLLA …

The Expression And Function Of Phosphacan/Rptpβ In Adaptive Synaptogenesis After Traumatic Brain Injury, Janna Harris

Theses and Dissertations

Traumatic brain injury (TBI) affects 1.5 million Americans annually and is a major health concern. Increasing evidence suggests that the brain extracellular environment regulates plasticity and synaptic recovery following TBI. Here we have focused on phosphacan/RPTPβ, an alternatively spliced group of chondroitin sulfate proteoglycans which are prominent within the mature brain extracellular matrix (ECM). Previous studies show that phosphacan/RPTPβ influences neuronal migration, adhesion, neurite outgrowth, and morphogenesis. However, our understanding of how these important ECM components are involved in recovery from brain trauma remains unclear. In the present study, we used unilateral entorhinal cortex lesion (UEC), a model which induces …

Evolution Of Picornaviruses: Impacts Of Recombination And Selection, Nicole Noel Lewis-Rogers

Theses and Dissertations

Picornaviruses are responsible for some of the most common and debilitating diseases affecting humans and animals worldwide. The objectives of this dissertation research were (1) estimate phylogenetic relationships among 11 picornavirus genera and within three species: foot-and-mouth disease virus (FMDV: Aphthovirus) which afflicts cloven-hoofed animals and human rhinovirus A and B (HRV: Enterovirus) which cause the common cold; (2) better understand the impact recombination has on genomic organization and evolution; (3) characterize where positive and purifying selection has occurred in proteins and how selection has influenced phenotype. The dissertation includes four studies. The first chapter provides an overview of the …

Hair, Feces And Breath Isotope Fractionation In Alpacas (Llama Pacos), Llamas (Llama Glama) And Guanacos (Llama Guanacoe) From Bolivia And Chile, Lino Constancio Lopez Lopez

Theses and Dissertations

This study was conducted to determinate carbon and nitrogen isotope fractionation in Bolivian and Chilean alpaca, llama and guanaco, hair, breath, plasma and feces. We also wanted to determine forage selection for these camelids using stable isotope technology. From the data, niche feeding and diet selection habits will determinate based on fecal composition. Bolivian sites were located near the high snow Altiplano Mountains at Tomarapi, Sajama, and at the Technical University Oruro's research center at Condoriri, Bolivia. Chilean samples were collected at INIA's (Instituto Nacional de Investigacion Agropecuaria) Kampenaike Research Station, Punta Arenas, Chile. Bolivian alpacas and llamas were 3 …

Specific Compartmentalization Of Iga Ascs In Mouse Salivary Glands Via Differential Expression Of Chemokines And Chemokine Receptors, Yuet Ching Law

Theses and Dissertations

The mucosal system, which forms a barrier between internal organ systems and the external environment, is frequently exposed to pathogenic microorganisms. Immunoglobulin A (IgA) antibody secreting cells (ASCs) localize in the lamina propria, and produce IgA antibodies which help protect mucosal tissues. The concept of a common mucosal immune system in which IgA ASCs have the ability to populate any mucosal site has been proposed (1, 2). However, recent research has suggested that IgA ASCs primed in different mucosal sites might possess different sets of chemokine receptors, and therefore migrate specifically to particular mucosal locations (3). In this study, the …

Ceramide Kinase And Ceramide-1-Phosphate, Dayanjan Wijesinghe

Theses and Dissertations

Ceramide-1-phosphate (C1P) is a bioactive lipid that has been implicated in many biological processes. Our laboratory has conclusively demonstrated its role in inflammation via activation of cPLA2α. The only known enzyme to date responsible for direct synthesis of C1P is ceramide kinase. Very little was known about this enzyme in terms of its enzyme kinetics and substrate specificity. As CERK is an enzyme that acts on membrane lipids, its kinetics cannot be studied using standard bulk dilutions methods. Thus we developed a surface dilution approach using Triton X 100 mixed micelles for studying the kinetics of CERK. We discovered that …

Corrosion Damage Studies Through Microscopy And Stress Analysis, Ronak Patel

Theses and Dissertations

Corrosion is the destructive result of chemical reaction between a metal or metal alloy and its environment. Airplanes, power generating plants, chemical process and manufacturing plants, concrete structure, and many others which widely uses aluminum alloy and stainless steel alloys are subjected to corrosion. The estimated cost of corrosion damage is in order of 3 to 5 percent of gross national product (GNP). Out of all forms of corrosion, pitting corrosion is most commonly observed in aluminum alloy 2024 and stainless steel type 316. There is a need to study the stress environment around the pits in order to predict …

Computer-Aided Trauma Decision Making Using Machine Learning And Signal Processing, Soo-Yeon Ji

Theses and Dissertations

Over the last 20 years, much work has focused on computer-aided clinical decision support systems due to a rapid increase in the need for management and processing of medical knowledge. Among all fields of medicine, trauma care has the highest need for proper information management due to the high prevalence of complex, life-threatening injuries. In particular, hemorrhage, which is encountered in most traumatic injuries, is a dominant factor in determining survival in both civilian and military settings. This complication can be better managed using a more in-depth analysis of patient information. Trauma physicians must make precise and rapid decisions, while …

Personality Factors Influencing Bemidji State University Industrial Technology Majors Making Heavy Industrial Construction Career Choices, Timothy M. Brockman

Theses and Dissertations

The purpose of this study was to apply Holland's theory and gather information about Bemidji State University (BSU) construction management (CM) students, CM graduates, CM faculty, and the heavy industrial construction workforce for a greater understanding of students, the academic environment, and the heavy industrial construction environment. A secondary purpose was to help increase recruitment and retention of students within the CM emphasis of the Industrial Technology major at BSU, and provide support for development of curricula within the CM emphasis as it meets needs of the construction industry. The study examined five research questions using Holland's Self-Directed Search (SDS) …

The Design And Synthesis Of Zinc(Ii) Ion Fluorescence Sensors, Julius Ngunde Ngwendson

Theses and Dissertations

This work is a study of a new class of aromatic amines as fluorescent zinc sensors. N-(Carboxymethyl)-N-[3-[(carboxymethyl)amino]-2-methoxy-5-methylphenyl]glycinate exhibits more than 20-fold fluorescence enhancement in the presence of Zn(II) ions when excited at 300 nm. This compound shows a fluorescence emission at 390 nm, which in the presence of Zn(II) ion exhibits a 20 nm emission blue shift. The tetraacetate version, potassium N,N'-(2-methoxy-5-methyl-1,3-phenylene)bis[ N-(carboxymethyl)]glycinate, exhibits a 7-fold sensitivity to Zn(II) ion, and there is no emission band shift observed. Investigations point to the methoxy group for the ratiometric behavior and increased sensitivity to zinc. The diacetate versions—sodium N-(carboxymethyl)- N-(2-methoxyphenyl)glycinate, sodium N,N'-(2-methoxy-5-methyl-1,3-phenylene)bisglycinate …

Women, Doctoral Degrees, And Technology: Female Doctoral Students Online, Adonica Schultz Aune

Theses and Dissertations

Women, Doctoral Degrees, and Computer Technology: Female Doctoral Students Online was conducted at a Midwestern university addressing the questions: (1) How do women use the computer during their doctoral work? (2) Why do women use the computer for their doctoral work? (3) When do women working on their doctoral work use the computer? and (4) Has the personal computer enabled the doctoral student to complete her Ph.D.? The investigation was conducted in three stages. Phase one was a pilot survey of 23 women in doctoral programs that provided descriptive data on how, how much, and when women in doctoral programs …

Two Approaches To The Removal Of Heating Fuel Oil Trapped In Wood And Concrete: Bio- And Photoremediation. Characterization Of Lignans In Flaxseed By Hplc/Dad/Esi Tof Ms And Gc/Ms, Inna Eduardovna Popova Tyapochkin

Theses and Dissertations

Chemical contamination of buildings occurs as a result of storage, spills, and catastrophic floods. Over the time, contaminants slowly evaporate from the structural elements posing health risks. To develop a cost-effective and safe route to decontaminate and reuse the contaminated wood and concrete, studies including extraction of fuel oil hydrocarbons from wood and concrete, their transport and remediation were conducted. n-Hexadecane was quantative recovered from wood using sequential extraction with acetone followed by pentane; from ambient concrete using pentane; and from water-saturated concrete using 2-propanol. Transport of fuel oil hydrocarbons in wood can be described by a two-step model: fast …

Tumor Necrosis Factor Alpha Modulates Nmda Receptors In The Central Nervous System, Javier H. Jara

Theses and Dissertations

Multiple cytokines are secreted in the brain during inflammatory conditions and likely affect neuronal survival. Previous studies demonstrated that glutamate and tumor necrosis factor alpha (TNFα) kill neurons via activation of the NMDA and TNFα receptors, respectively. Nevertheless the mechanisms of neuronal death are not completely elucidated. The goal of this study was to characterize the signaling cross-talk pathway initiated during this inflammation-related mechanism of cell death. Stimulation of mouse cortical neuron cultures with TNFα resulted in a transient increase in NMDA receptor-dependent calcium influx that is additive with NMDA stimulation and inhibited by pretreatment with the NMDA receptor antagonist. …

Semantic Role Agency In Perceptions Of The Lexical Items Sick And Evil, Nathan G. Simmons

Theses and Dissertations

Inspired by an ongoing debate in the clinical sciences concerning the value of evil as a label for human behavior (Mowrer 1960, Staub 1999, Wellman 2000, Williams 2004 etc.), this thesis examines the semantic role of AGENT in the lexical items sick and evil. Williams makes the argument that the label evil removes responsibility from the doctor, whereas, the label sick empowers the doctor in bringing about a cure. While this view is not universally accepted in the field, it does bring to light an interesting question in applied linguistic semantics as to the assignment of agency with respect to …

Comprehension And Retention: The Effect Of Concrete Details And Causal Structure In Scientific Narrative, Wendi Michelle Wilcken

Theses and Dissertations

The purpose of this study was to examine two of the salient elements of instructional narratives as a guide to instructional practice. The literature summarized in this report discusses the theoretical basis for narrative impact on comprehension and retention, enumerates and defines possible salient narrative elements from the literature, and examines the instructional impact of two of these elements: concrete details and causal structure. This is intended to help provide guidance to instructional designers and teachers who desire to use narrative in science instruction. Participants included 94 high school physics students. An experimental research design of 2 (Gender) x 2 …

Angiogenic Potential Of Human Macrophages On Electrospun Bioresorbable Vascular Grafts, Koyal Garg

Theses and Dissertations

The aim of this study was to investigate macrophage interactions with electrospun scaffolds and quantify the expression of vital angiogenic growth factors in vitro. This study will further help in evaluating the potential of these electrospun constructs as vascular grafts for tissue repair and regeneration in situ. Human peripheral blood macrophages were seeded in serum free media on electrospun (10 mm) discs of polydioxanone (PDO), elastin and PDO:elastin blends (50:50, 70:30 and 90:10). The growth factor secretion was analyzed by ELISA. Macrophages produced high levels of vascular endothelial growth factor (VEGF) and acidic fibroblast growth factor (aFGF). Transforming growth factor …

Rethinking The Historical Lens: A Case For Relational Identity In Sandra Cisneros's The House On Mango Street, Annalisa Wiggins

Theses and Dissertations

My thesis proposes a theory of relational identity development in Chicana literature. Gloria Anzaldua's Borderlands/La Frontera offers an interpretation of Chicana identity that is largely based on historical models and mythology, which many scholars have found useful in interpreting Chicana literature. However, I contend that another text, Sandra Cisneros's The House on Mango Street, not only illustrates the need for an alternative paradigm for considering identity development, but in fact offers such an alternative. I argue that Cisneros shows a model for relational identity development, wherein the individual develops in the context of her community and is not determined solely …

Photocycloaddition Of Allenes, Crystal Ward

Theses and Dissertations

For the past ten years the Fleming research group has been involved in the research of allene compounds. We have explored the synthesis of allenes as well as several reactions involving allenes, such as oxidation and nitrene addition. Recently, we have explored the synthesis and photochemistry of allenic alcohols tethered to silicon. There are literature examples using allenes in synthesis, but very few examples exist using allenes in photochemical cycloaddition reactions. We have found that a diisopropylallenyloxy(cinnamyloxy)silane derivative undergoes [2+2] photochemical cycloaddition to produce a cyclobutylsilane product when irradiated for 60-75 minutes.

Selective Non-Peptide Mu-Opioid Receptor Antagonist: Design, Synthesis And Biological Studies, Lindsey Aschenbach

Theses and Dissertations

There are currently many opioid agonists available for clinical use as analgesics. However, many of these opioid agonists have notorious side effects including respiratory depression and may lead to addiction and dependence. Problems associated with these opioid agonists are determined to come from their interactions with the mu-opioid receptor. Opioid antagonists, such as naltrexone, have shown to aid in the treatment of opioid addiction. Although naltrexone has high affinity to the mu-opioid receptor, it lacks selectivity. Novel selective mu-opioid receptor antagonists were designed based on the identification of important pharmacophore elements in several known mu-opioid receptor agonists and antagonists. These …

Physical Mapping Of Ribosomal Genes In New World Members Of The Genus Chenopodium Using Fluorescence In Situ Hybridization, Maria C. Sederberg

Theses and Dissertations

The genus Chenopodium contains many economically important species in the New World, but is relatively understudied and poorly understood, especially in terms of evolutionary relationships. A better understanding of the structure of this genus could significantly help in breeding efforts on its cultivated members, notably the tetraploid C. quinoa and also certain varieties of C. berlandieri, also tetraploid. Of special concern is determining which diploid weed species are the most likely ancestors for C. quinoa, C. berlandieri, and the other tetraploid members of subsection Cellulata. The phylogeny can be understood in part by examining the ribosomal RNA loci and observing …

Avian Dispersal Of The Actinomycete Frankia Across A Barrier Island Landscape, Spencer Bissett

Theses and Dissertations

In the nutrient-poor soils characteristic of coastal environments, symbiotic association with the nitrogen-fixing root endosymbiont Frankia is essential to establishment and survival of the woody shrub Morella cerifera. Nutrient deficiency quickly becomes severe unless seedlings are infected by Frankia soon after germination. However, the means of arrival of Frankia prior to shrub establishment has not been determined. Using sterilized lab-grown M. cerifera seedlings and fecal samples collected from passerine birds on the Eastern Shore of Virginia, viability of avian dispersal of the bacteria was tested. Although passerine fecal samples did produce nodules on some sterilized M. cerifera seedlings, these experimental …

Autism And Misdiagnosis: Is Early Detection Always Accurate?, Melissa Hosier

Theses and Dissertations

Autism is a brain-based disorder that involves disrupted social interactions and communication development along with stereotyped patterns of behaviors and interests. Early detection and intervention is crucial for children diagnosed with autism. While current trends in research and detection are leaning toward earlier diagnosis of the disorder, limited research exists to support that earlier diagnoses are accurate. The present study utilized archival research and interviews to examine the accuracy of early diagnosis in children identified as autistic using DSM-IV criteria, standard screening and diagnostic tools and genetic testing. Results indicated that genetic testing was the most reliable tool for accurately …