Digital Commons Network^™

Student Content Knowledge Increases After Participation In A Hands-On Biotechnology Intervention, Amber L. Bigler

Theses and Dissertations

Hands-on learning is at the heart of science learning. This study examined increased changes of student content knowledge in biology, particularly biotechnology, after a hands-on biotechnology intervention was implemented into a secondary school. A traditional learning school was selected for a control. Both teachers had participated in a biotechnology professional development program called Project Crawfish. Students from both schools took the same assessment before and after their respective units (biotechnology intervention and genetics unit), and the classroom was the unit of analysis (n=5, n=6, respectively). The assessment was compared as a whole and then divided into five components, eight questions …

An Indecent Obsession, Aaron Mcintosh

Theses and Dissertations

The title of my thesis is appropriately borrowed from a romance novel title, as my work proposes to mine the content, design and culture of romance novels and other erotic texts in order to excavate my own queer romance narrative. The body of work includes large-scale drawings of “stand-in boyfriends” stolen from romance novel covers, pieced fabric text works based on the titles of erotic texts, and a couch covered in erotic reading material. Drawing attention to the ubiquity of heterosexualized images and texts by deconstructing them, my work critically questions larger social constructions of normality and deviance, pleasure and …

The Relationship Between Treatment-Seeking And Characteristics Of Depression Among African Americans, Tamara Scott

Theses and Dissertations

Objective: Depression is a common psychiatric condition, and despite the availability of effective treatments, this condition is largely under-recognized and undertreated, particularly among African Americans. One reason for this discrepancy may be that depressive symptoms often co-occur with physical health conditions, which can influence both the reporting of depressive symptomology, willingness to seek care, and the type of healthcare provider sought. The purpose of this study was to explore the relationship between treatment-seeking characteristics and diagnostic attributions of depression among community-dwelling African American adults. Methods: Data come from the National Survey of American Life, a nationally representative sample of African …

Self-Ligating Vs. Conventional Brackets As Perceived By Orthodontists, Chase Prettyman

Theses and Dissertations

Introduction: Within the past decade, significant developments, new designs, and numerous proposed advantages of self-ligating (SL) brackets have caused them to gain great popularity among practicing orthodontists. The purpose of this study was to determine if there are significant clinical differences between SL and conventional brackets on orthodontic treatment as perceived by practicing orthodontists, and more specifically, if the proposed advantages of SL brackets are evident in clinical practice. Methods: A survey was developed to evaluate how SL brackets compare to conventional brackets when perceived by practicing orthodontists (n=430). The initial series of questions focused on individual practitioner characteristics and …

An Ensemble-Based Computational Approach For Incremental Learning In Non-Stationary Environments Related To Schema- And Scaffolding-Based Human Learning, Ryan Elwell

Theses and Dissertations

The principal dilemma in a learning process, whether human or computer, is adapting to new information, especially in cases where this new information conflicts with what was previously learned. The design of computer models for incremental learning is an emerging topic for classification and prediction of large-scale data streams undergoing change in underlying class distributions (definitions) over time; yet currently, they often ignore significant foundational learning theory that has been developed in the domain of human learning. This shortfall leads to many deficiencies in the ability to organize existing knowledge and to retain relevant knowledge for long periods of time. …

Neuroprotective Effects Of Postinjury Lithium Treatment: Determining The Optimal Dosing Paradigm And Assessing Potential Mechanisms Of Action, Katharine Eakin

Theses and Dissertations

Traumatic brain injury (TBI) has a dramatic impact on our society in terms of mortality, morbidity, and inherently high financial costs. Formidable research efforts are being addressed to the identification of neuroprotective agents capable of ameliorating the neurological outcome after TBI. Preclinical studies have recently demonstrated lithium to be a promising neuroprotective agent for both acute ischemic brain injury and chronic neurodegenerative disease. In light of these encouraging data, we designed a lateral fluid-percussion injury (FPI) study aimed at investigating the role of early post-traumatic administration of lithium as a strategy for reducing TBI-induced motor and cognitive deficits. The optimal …

Use Of Cone Beam Computed Tomography In The Determination Of Mental Foramen Location In Relation To Mandibular 1st And 2nd Premolars, Sanjay Bhagchandani

Theses and Dissertations

The purpose of this study was to use existing Cone Beam Computer Tomography images to determine the vertical and horizontal location of the mental foramen in relation to the mandibular first and second premolars, as well as the distance in millimeters from the apex of each. The distance from the inferior border of the foramen to the inferior border of the mandible was also recorded. The average distance from the apices of the mandibular first and second premolars to the mental foramen was 7.43mm (SD= 1.97) and 5.30mm (SD=1.65) respectively. The foramen was located 11.44mm (SD=1.49) from the inferior border …

A Case Study On Sibling Rivalry And The Use Of A Social Skills Training Model, Michelle Maiorano

Theses and Dissertations

The purpose of this study was to use social skills training on an individual child to enhance the relationship with her siblings. The social skills sessions focused on factors such warmth, rivalry, conflict and status/power between the two siblings. These were measured by the Sibling Rivalry Questionnaire which the mother filled out before the sessions began and after they were completed. The results did not find a major difference between the pre and post tests. Warmth increased slightly, rivalry decreased slightly, there was no difference in the amount of conflict, and there was a slight decrease in relative status/power from …

African American Children Living In Urban Environments: An Investigation Of Early Literacy And The Influence Of Psychological Strengths And Family Support, Pia Stanard

Theses and Dissertations

Literacy is a basic fundamental skill for academic, professional, and social success in our culture. Children with low exposure to reading can experience reading difficulties, diminished cognitive development, and poor academic outcomes. Inconsistency in the conceptualization of early literacy has hampered research and development of successful, translational early literacy interventions, particularly for children from low-income households. Preschoolers from low-income, urban backgrounds (n = 426), including 221 females and 205 males aged 35 - 60 months (M = 47.46, SD = 6.44) participated in an investigation of the latent factorial structure of early literacy. The study also explored whether children’s psychological …

The Association Between Prenatal Care Content And Quality With Preterm Birth And Maternal Postpartum Health Behaviors, Susan Cha

Theses and Dissertations

Background: Health policies that seek to improve pregnancy outcomes focus on increasing the availability and access to prenatal care (PNC) services based on studies that support an association between insufficient PNC and adverse birth outcomes. These studies employ PNC utilization indices that measure the adequacy of PNC use, but these indices fail to account for the content or specific components of PNC. Objectives: The purpose of this study was to utilize PRAMS and birth certificate data to evaluate the content and quality of PNC in Virginia, and its impact on preterm birth and maternal postpartum health behaviors. Methods: Data was …

A Computational Biology Approach To The Analysis Of Complex Physiology: Coagulation, Fibrinolysis, And Wound Healing, Nathan Menke

Theses and Dissertations

The birth of complexity research derives from the logical progression of advancement in the scientific field afforded by reductionist theory. We present in silico models of two complex physiological processes, wound healing and coagulation/fibrinolysis based on two common tools in the study of complex physiology: ordinary differential equations (ODE) and Agent Based Modeling (ABM). The strengths of these two approaches are well-suited in the analysis of clinical paradigms such as wound healing and coagulation. The complex interactions that characterize acute wound healing have stymied the development of effective therapeutic modalities. The use of computational models holds the promise to improve …

Is Food Insecurity A Contributing Factor To Childhood Obesity? The Association Of Household Food Insecurity And Obesity Prevalence Among Children And Adolescents In The United States, Zarah Liz Panaguiton

Theses and Dissertations

The obesity epidemic is a major public health concern, where the prevalence rates amongst the American children population have more than doubled since the 1980s. Among overweight children, the risk of becoming an overweight or obese adult is 70% higher than children of normal weight, and obese children are more likely to remain obese into adulthood and face a number of morbidities associated with it, including lower quality of life and increased financial burden. In this research, we examined the relationship between household food security and obesity among children and adolescents between the ages of 2-18 years old. We used …

Endodontic Education And Educators: Who Is Teaching Our Predoctoral Students And What Methods Are Being Taught?, Ellison Turner

Theses and Dissertations

A survey was conducted to assess the current state of predoctoral endodontic education in the United States. Fifty-one schools received surveys and 73% responded. Seventy-six percent were from public schools with most having a graduate endodontic program (84%). Preclinical training most often began in the spring of second year and clinical training started in the fall of third year (each 54%). All programs that responded stated that endodontists routinely instruct students in the clinic. Sodium hypochlorite 2.5% was the irrigation of choice. Calcium hydroxide was the most common intracanal medicament. Cold lateral condensation with gutta-percha was the most frequently indicated …

Biobehavioral Mechanisms Of Emotion And Hiv Disease: Exploring Potential Mediators Of The Relation Between Trait Positive And Negative Affect And Hiv Health Status, Karen Stewart

Theses and Dissertations

Considerable research supports an association between negative psychosocial functioning and adverse health outcomes. The biobehavioral model is well supported and posits that these effects occur via alterations in physiological response and health damaging behaviors. Evidence is accumulating about potential benefits of positive psychosocial functioning; however, less is known about the mechanisms of these effects. The broaden-and-build model of positive emotions holds that positive emotions can undo the physiological and behavioral restrictions associated with negative emotions and promote resource development. The present correlational study sought to explore whether cortisol, medication adherence, and health behaviors (smoking, alcohol use, physical activity, and nutrition) …

Spinal Cord Injury: Exploring The Histology Of Electrospun Implants In Vivo, Charles Lin

Theses and Dissertations

Spinal cord injury results in loss of motor function and sensory perception. A myriad of obstacles prevent axonal regeneration and ultimately functional recovery in those afflicted with spinal cord injury. Combinatorial strategies addressing many of these obstacles simultaneously have shown promising results. Laboratories investigating contusional spinal cord injuries must overcome the formation of a fluid filled cyst, a physical gap that axons must traverse, at the injury epicenter. To fill the cyst, our lab has generated a 3-D electrospun matrix that is capable of directing neurite outgrowth, delivering neurotrophic support, and reducing the activity of neuroinhibitory compounds. These electrospun matrices …

The Epidemiology Of Snakebite Injury In The Amazonian Regions Of Ecuador, Sarah Stuppy

Theses and Dissertations

The epidemiology of snakebite injury in the Amazonian regions of Ecuador Introduction: Morbidity and mortality from snakebite envenomations is a major public health issue in remote areas of under-developed countries. Several attempts have been made to approximate the impact of snakebites worldwide, however these are assumed to be underestimations due to the lack of documentation on the local level. Ecuador's Amazonian region lacks a comprehensive community-based surveillance system, however it is unique in that an aero-medical transport system is in place to transfer the patients requiring hospitalization to one of the five urban medical centers. Beginning in 1998, Servicio Aéreo …

A Novel Role Of Sirt1 In Sildenafil Induced Cardioprotection In Mice, Mona Shalwala

Theses and Dissertations

Phosphodiesterase-5 inhibitor, sildenafil (SIL) protects against myocardial ischemia/reperfusion (I-R) injury. We hypothesized that SIL-induced protection may be mediated through activation of SIRT1, an enzyme which deacetylates proteins involved in cellular stress response. Adult male ICR mice were treated with SIL (0.7mg/kg ip), Resveratrol (RSV) (5mg/kg ip) (positive control), or saline (0.2 ml ip). The hearts were harvested 24 h later and homogenized for SIRT1 activity analysis. Both SIL and RSV increased cardiac SIRT1 activity (P<0.001) as compared to Saline. Adult mouse ventricular cardiomyocytes pre-treated with either SIL or RSV (1µM) in vitro also upregulated SIRT1 activity (P<0.05). SIL also reduced infarct size following 30 min. ischemia and 24 h reperfusion in vivo. Sirtinol (5mg/kg in 10% DMSO, ip), a SIRT1 inhibitor abolished the infarct-limiting effect of SIL and RSV (P<0.001). In conclusion, activation of SIRT1 by SIL plays an essential role in cardioprotection against I-R injury.

A Qualitative Analysis Of Messages Conveyed In Parent-Adolescent Communication About Substance Use: Variations Along Dimensions Of Maternal And Familial History Of Substance Abuse, Nikola Zaharakis

Theses and Dissertations

Little research has examined the content of parent-adolescent communication about substance use and variables that may influence it. Using a grounded theory approach for secondary data analysis, qualitative data were drawn from a longitudinal study of coping and substance use in a sample of urban African American adolescents (N=132; M= 13.77 years) and their mothers in Richmond, VA. Transcripts of interviews with participants’ mothers regarding their conversations with their adolescent about alcohol, tobacco or other drugs were microanalyzed by two coders in three sets according to the youth participant’s maternal and familial history of substance abuse. Findings revealed considerable similarity …

Long-Term Spatial And Temporal Variability Of Benthic Macroinvertebrate Communities: Implications For Bioassessment Of Lotic Systems, Eve O'Connor

Theses and Dissertations

The structure and composition of benthic macroinvertebrate communities can vary spatially and over time. Spatial and temporal variation along a stream has many implications for population and community dynamics, which may influence bioassessment programs. I examined variability in the benthic community of eight streams within the Polecat Creek, Virginia watershed. These streams vary in size from 1st to 4th order. The streams were sampled once every season for eleven years using standard bioassessment protocols. Macroinvertebrates were sampled from both sediment and submerged wood habitats at each site. The coefficient of variation (CV) was used to quantify among season, among year …

Psycho Beach Party: A Vocal And Physical Exploration Of Gender, Megan Persinger

Theses and Dissertations

On September 24, 2009, Theatre VCU opened its production of Psycho Beach Party, written by Charles Busch and directed by Steve Perigard. In our production, two male actors, Tommy Callan and Kyle Cornell, were cross-sex cast to play female characters, Chicklet and Marvel Ann respectively. In addition to serving as vocal coach for the production, I was to help Callan and Cornell vocally transform into female characters. I have documented our exploration of gender, specifically the vocal transformation from male to female, in Theatre VCU’s production of Psycho Beach Party. Neither actor had played a female character onstage before, and …

Scribble: A Potential Dual Kinase Inhibitor, Steven Christofakis

Theses and Dissertations

Extracellular signal-regulated kinases (ERKs) modulate cellular activities in response to extracellular stimuli and play important biological roles. Thus, perturbed kinase pathways induce pathological conditions, such as tumor development. Rit, a novel member of the Ras family GTPases, activase ERK6, and its over-expression confers tumorigenicity. We hypothesized the presence of scaffolding molecules specific to ERK6, similar to other known MAP kinases. We performed yeast two-hybrid assays using ERK6 as bait, and Scribble was identified as a binding partner. Scribble contains 16 LRR domains and four PDZ domains. We performed immunoprecipitation (IP) assays and discovered ERK2 as another binding partner. Surprisingly, no …

Phosphorylation Of The Type Iα Regulatory Subunit Of Camp -Dependent Protein Kinase., Karen Mae Boeshans

Theses and Dissertations

The mechanism by which the type la regulatory subunit (RIa) of cAMP-dependent protein kinase (PKAI) is localized to cell membranes is unknown, however, RIa may be localized through hydrophobic interaction with membrane lipids. To determine if post-translational lipid modification of RIa is important for membrane association, both beef skeletal muscle cytosolic RI and beef heart membrane-associated RI were characterized by electrospray ionization mass spectrometry. Total sequence coverage was 98% for both the membrane and cytosolic forms of RI after digestion with trypsin or AspN protease. Sequence data indicated that membrane-associated and cytosolic forms of RI were the same RIa gene …

Mutation Of The Vesicular Monoamine Transporter-1 Gene Alters Its Protein Product, Abena Watson-Siriboe

Theses and Dissertations

The vesicular monoamine transporter 1 (VMAT1) is essential for storage of monoamines, such as epinephrine and serotonin, in secretory vesicles of neuroendocrine cells. Recently the VMAT1 protein was detected in human and mouse brain, and mutations of the VMAT1 gene at single DNA nucleotides (single nucleotide polymorphisms or SNPs) were associated with schizophrenia. In this study, Chinese hamster ovarian cells were stably transfected with either human VMAT1 DNA (GenBank: #NM_003053.1 or DNA with the Thr4Pro SNP, which results in a threonine to proline change in amino acid number 4 of the VMAT1 protein. Western blot analysis revealed that cells with …

Assessing Scientific Inquiry: Teacher Beliefs And Practices, Adam James Mitchell

Theses and Dissertations

Science education reform movements have long urged the use of inquiry methods in all science instruction. More recently, standards and accountability reform efforts have emphasized measuring and improving student science achievement. Researchers have questioned the alignment and balance between these reforms (Lane, 2004; Yeh, 2001). This study addresses issues faced by secondary science teachers as they simultaneously meet the goals of these reform movements. Mixed methods were used to answer the questions: 1) Can a teacher's beliefs and practices regarding inquiry teaching methods be correlated with his/her assessment practices?; 2) What item types are most commonly employed by teachers that …

The Aortic Valve Endothelial Cell: A Multi-Scale Study Of Strain Mechanobiology, Scott Andrew Metzler

Theses and Dissertations

The aortic valve (AV) functions in arguably the most demanding mechanical environment in the body. The AV experiences fluid shear stress, cyclic pressure and mechanical strain in vivo. Recent evidence has shown the progression of degenerative aortic valve disease (AVD) to be an active cellular mediated process, altering the conception of the AV as a passive tissue. AVD has shown a strong correlation with altered hemodynamics and tissue mechanics. Aortic valve endothelial cells (AVECs) line the fibrosa (aortic facing) and ventricularis (left ventricle facing) surfaces of the valve. AVECs sense and respond to circulating stimuli in the blood stream while …

Negotiating The Gray Areas Of Ethical Decision Making: Deaf Therapists Working In The Deaf Community, Denise Alice Thew

Theses and Dissertations

Deaf and Hard-of-Hearing psychologists, who provide services to the Deaf and Hard-of-Hearing community, face unique dilemmas when dealing with overlapping relationships. Similar to psychologists from other small or rural communities, dual relationships are generally unavoidable. The ethical guidelines for psychologists do not provide for the exceptional struggles that many Deaf therapists face in this regard.

This study was conducted to explore the decision-making patterns that Deaf therapists have used in negotiating nonsexual, multiple relationships with clients. Ten taped interviews were conducted to collect preliminary data on the overlapping relationship dilemmas of participants who are Deaf, licensed therapists and have been …

Assessment Of Mature Rural Adults Experiencing Depression, Penny Briese

Theses and Dissertations

Depression is well recognized worldwide as a leading cause of disability which reduces quality of life, particularly among mature adults. The purpose of this paper is to disseminate the finding of a graduate project which explored depression in mature adults, with a focus on how to improve healthcare for this population in rural communities in North Dakota. A literature review was performed in order to compare rural versus urban practices in the assessment, diagnosis, and treatment of depression in mature adults. Medical professionals working in the fields of clinic, hospital, and emergency department healthcare were surveyed as to their current …

Sense Of Connection And Learning In Traditional And Online Courses At A Rural-Serving Community College, Jayne M. S. Kiner

Theses and Dissertations

The purpose of this study was to establish an overall measurement for student sense of classroom community in terms of the theoretical dimensions of emotional connection, and needs fulfillment (learning). Further intent was to determine the nature of differences, if they exist, between delivery method (traditional or online), teaching style cluster (containing formal authority style or facilitator style), course type, student’s residence (on-campus, off-campus commuting, or off-campus not commuting), age, and student major on sense of classroom community in terms of emotional connection and learning.

Quantitative research methods were used with a causal-comparative design and survey instruments. Differences were determined …

What Is The Evidence That Increasing The Length Of Breastfeeding Has On Risk Reduction Of Breast Cancer, Jaci K. Phillips

Theses and Dissertations

The focus of this study was to review the literature to determine if breast cancer incidence was reduced by breastfeeding duration. It is important for health care providers to present the benefits of breastfeeding to women of childbearing age and especially to those who are pregnant. This literature review will result in a pamphlet that will be available to health care providers to present to prenatal patients. Informing women about the benefits of breastfeeding and breast cancer risk reduction will promote their ability to make an informed decision on breastfeeding. The Health Belief Model is the framework that was used …

Normalizing The Immediate And Early Postpartum Period: A Teaching Module For Nursing Students, Cate Edlebeck

Theses and Dissertations

No abstract provided.