Digital Commons Network^™

The Design And Implementation Of A Modular Tissue Engineering Test Bed To Pursue An Alternate Pathway Of Cell Culture Based Research, Ryley Patrick Griffin

Theses and Dissertations

The development of a Modular Automated Tissue Engineering Test Bed (MATETB) is the next step in the development of cheap and easily available in vitro cell regulatory systems for both academic and industrial purposes. This project outlined the framework and requirements for a device that could automatically regulate and collect data on a cell culture through the manipulation of its liquid environment. From this framework, a prototype was built. The device would fulfill cell culture needs while collecting data automatically and allowing for live viewing and imaging of cells. The device was comprised of a mechanical skeleton (MS), an environmental …

Demonstrating Feasibility Of Ultrasound-Sensitive Microbubbles Delivery To Treat Disruptions In The Blood Brain Barrier Endothelium, Rubens Jourdain

Theses and Dissertations

Vascular cognitive impairment and dementia (VCID) is one of the main causes of dementia. VCID is caused by age and other genetic factors that lead to disruptions in the blood brain barrier (BBB) and allow blood components to enter the brain. The currently available treatments of VCID include opening the BBB with high-frequency-ultrasound to deliver drugs to the brain. However, this only treats the already developed disease. Consequently, we developed a new drug delivery system whose goal is to restore the brain capillary endothelial cells (BCEC) lining the blood vessel. The objective of this study was to demonstrate the feasibility …

Advancing Biofabrication Methodologies To Develop Biomimetic Tissue Scaffolds For Musculoskeletal Applications, Nashaita Yezdi Patrawalla

Theses and Dissertations

Musculoskeletal injuries impose a significant global burden, affecting millions and generating an economic toll exceeding $14 billion. Tissue engineering has gained traction as a promising strategy offering potential for functional restoration of damaged musculoskeletal tissues. This research aims to advance biofabrication techniques for generation of biomimetic tissue scaffolds, by optimizing scaffold design and modulating fabrication process parameters to generate scaffolds for musculoskeletal regeneration with application-specific tailored properties. In this realm, collagen-based biomaterials have been extensively investigated for tendon and ligamentous applications, and in combination with bioceramics incorporated into the collagen framework to produce composite scaffolds that better recapitulate the native …

The Catalytic Conversion Of Corn Stover Sugars Into Lactic Acid, Ahmed Essam

Theses and Dissertations

The rising global demand for addressing global warming necessitates the development of renewable-based alternatives to current fossil fuel-based chemicals and fuels. Lignocellulosic biomass is the most abundant energy source following fossil fuels. As a result, agricultural residues such as corn stover have attracted substantial interest for utilization as a potential feedstock for renewable chemicals.Previous research work at the University of North Dakota showed promising results in converting corn stover sugars into chemical building blocks. The most crucial of these chemicals is lactic acid, which can be used in multiple applications including food, pharmaceuticals, and biodegradable plastics. Traditionally, lactic acid is …

Fluorescence Imaging Systems To Detect Contamination On Surfaces Common In Food Processing And Medical Facilities, Benjamin Hu

Theses and Dissertations

Biofilm are aggregates of microbial cells encased under an extracellular polymeric substance (EPS) and resist sanitizers or antibiotics. Because of their persistence on various surfaces such as medical devices or industrial piping, biofilm formation is associated with approximately 65% of all microbial infections. Thus, their detection and deactivation are of great importance to public health. Many detection methods such as swabbing require sampling by rubbing , which can be inefficient and challenging. This study proposes the use of handheld multispectral fluorescence imaging systems to detect and deactivate biofilm in situ. We utilized the Contamination and Sanitization Inspection (CSI) and -Disinfection …

Cyclical Musicians: Collegiate Music Students’ Experiences Of Instrumental Musicking Processes Across The Menstrual Cycle, Anne Marques Catarin

Theses and Dissertations

Instrumental music researchers tend to focus on non-sex-specific health topics, such as posture (e.g., Garnett et al., 2022; Rousseau et al., 2023), musculoskeletal health (e.g., Mizrahi, 2020; Pratt et al., 2020), or neurological movement disorders (e.g., Altenmüller & Jabusch, 2010; Frucht, 2009). Despite the considerable extent of topics covered in musician health, the role of menstrual health in instrumentalists is currently underdeveloped. Using a case study design (Stake, 1995; Yin, 2014), I explored the unspoken experiences of collegiate instrumental music majors who menstruate, in an investigation to better understand the role of menstrual cycles in their musicking. For four months, …

Development Of Fluorescent-Based Nanoparticles For Bioimaging, Gene Regulation Expression, And Photo-Induced Synergistic Cancer Therapy, Di Sun

Theses and Dissertations

Fluorescent-based nanoparticles have been applied to a broad range of biological applications, as discussed in this dissertation, including bioimaging, gene regulation expression, and photo-induced synergistic cancer therapy. Prior to applications, this dissertation first focused on the development and characterization of several fluorescent-based nanoparticles using different methods. These include 1) Streamlined synthesis of dual-emissive fluorescent-based silicon quantum dots (SiQDs). The SiQDs were generated as a promising, non-toxic labeling tool for bioimaging of a variety of cell types. both in visible and near-infrared (NIR) ranges. 2) Polymer-based nanoparticles - Polymer dots (Pdots) with a strong NIR fluorescence. The Pdots were functionalized as …

“How Can I Teach When I Was Not Taught How?”: Teacher Education For Mother Tongue Instruction For Stem In Southwest Nigeria, Motunrayo Adeosun

Theses and Dissertations

In this qualitative study, the experiences, training, and challenges of teachers in primary schools in a community in southwest Nigeria were explored. The purpose of this study was to identify the experiences of teachers about their training for mother tongue instruction and how they teach pupils with the mother tongue or local language of the community, the challenges faced by teachers in teaching with the mother tongue, and the strategies used by teachers to overcome the challenges. This study found that teachers received no training and there is no curriculum for the training of teachers to teach in the mother …

Parp1 Modulates Gata3-Mediated Gene Regulation In Breast Cancer, Mikhala Cooper

Theses and Dissertations

In eukaryotic cells, chromatin acts as a physical barrier for many transcription factors, preventing them from efficiently binding to their recognition sequences within the nucleosome. Pioneer factors are a class of transcription factors that bind to nucleosomes and activate silent chromatin. Pioneer factors are frequently involved in various immune cell pathways, tissue development, and homeostasis, thereby underscoring their important roles in development and disease. Previous research found that pioneer factors have at least three fundamental properties: 1) they can interact with their cognate recognition sequences before transcription activation, 2) they can increase local chromatin accessibility, and 3) they have integral …

Fabrication And Charcaterization Of Pullulan-Collagen Nanofibers Via Forcespinning, Valeria Leon Leal

Theses and Dissertations

Extensive research on collagen has spurred advancements in tissue engineering and biomaterial development, given collagen´s pivotal role in regulating various tissue structures across diverse organisms. This study delves into fabricating nanocomposites utilizing collagen, along with silver nanoparticles, chondroitin sulfate and pullulan, for potential applications in antibacterial medical devices, and cell regeneration. Collagen, abundant in the extracellular matrix, provides essential structural support, while silver nanoparticles and chondroitin confer antimicrobial properties, crucial for combating infections. Pullulan, a polysaccharide polymer, serves as a biocompatible carrier for silver nanoparticles whilst being the starting nanofiber forming material. Utilizing Forcespinning® technology, composite nanofibers were successfully synthesized. …

Assessing The Influence Of Native And Invasive Grasses On The Behavior Of Aedes Aegypti In South Texas, Emily Medelez

Theses and Dissertations

Invasive grasses in South Texas can have widespread negative effects, displacing native species, reducing biodiversity, and posing risks to public health. These grasses may flourish in moist, shaded environments, which not only support their growth but also creates habitats favorable for mosquito activity. This can increase the population and alter the behavior of mosquitoes, potentially raising the risk of disease transmission. In this thesis, I look at the behavior of mosquito species Aedes aegypti, primary vector of the yellow fever virus. I analyzed the influence of oviposition, flight and resting behaviors. I tested the relative attraction of Aedes mosquitoes …

Microwave-Assisted Green Synthesis Of Spiroindolinopyranopyrazoles, Esther Owusu

Theses and Dissertations

Green Chemistry, usually known as sustainable chemistry, is widely implemented worldwide to safeguard human health and the environment. Recent investigations in organic synthesis and chemical transformation have demonstrated that green chemistry techniques are significantly reducing both chemical waste and reaction time. In order to fulfill this ecological requirement, it is crucial to lessen the utilization of perilous solvents and noxious chemicals, while also creating innovative reaction methods that decrease the production of trash. The implementation of such methodologies can greatly enhance the field of green chemistry. A more environmentally friendly synthetic method was utilized to generate spiro chemicals and their …

The Lasting Psychological Effects Of Juvenile Arrests, Nadia Olivia Bortolin

Theses and Dissertations

This research investigated the enduring psychological consequences, specifically depression and anxiety, stemming from juvenile arrests and their influence on adulthood. Literature has demonstrated impacts on juvenile arrests from cultural, socioeconomic, family dynamics, and environmental elements. This study addresses a notable gap in understanding the long-term impact on mental health and employment. Research indicates a sensitivity to outcomes based on the age of arrest, with early adolescent arrests having profound effects on development and an increased likelihood of subsequent criminal activities. Developmental and risk factors, such as socioeconomic challenges and pubescent difficulties, contribute to persistent delinquent behaviors into adulthood. Some studies …

Novel, Ultra-Fast Electrochemical Sensors For The Enhanced Detection Of Neurotransmitters And Toxic Heavy Metals In Aqueous Solutions, Noel Manring

Theses and Dissertations

Neurodegenerative diseases (NDDs) are a growing global health concern, affecting millions of people worldwide. NDDs arise from the progressive loss of neuronal cell function, ultimately leading to cell death. Abnormal levels of neurotransmitters, the chemical messengers in the brain, have also been linked to NDDs. Despite various treatments and medications aimed at alleviating physical and mental symptoms, there is currently no cure or means to halt disease progression. While the exact pathogenesis of NDDs remain poorly understood, cognitive decline is largely attributed to brain volume reduction, particularly in the hippocampus. Clinical studies suggest that environmental factors, such as exposure to …

Synthesis Of Photocleavable Molecules For Neurological Applications, Nishal Madujith Egodawaththa Arachchilage Don

Theses and Dissertations

In recent biomedical research, the precise control of molecular dynamics through light activation has emerged as a powerful tool, particularly in the field of neurobiology. This approach involves the use of photocleavable cages or photoprotective groups (PPGs) that are chemically tethered to biologically active molecules such as neurotransmitters or calcium chelators. These cages remain inert until exposed to specific wavelengths of light, typically in the visible range, triggering the release of the active compound with high spatiotemporal precision.

For neurotransmitter systems, such as glutamate (Glu), which plays a critical role in synaptic transmission and memory formation, researchers have developed novel …

Impact Of State-Level Family Policies On Female Labor Force Participation And Entrepreneurship, Kathryn Staczek Rudloff

Theses and Dissertations

This dissertation aims to investigate the impact of state-level family policies on women's participation in the labor force in the United States, focusing specifically on the rise of self-employment and solopreneurship among women. The research seeks to answer the following question: "How do family policies at the state level impact participation of American women in the labor force?"

The study utilized a convergent parallel mixed-methods design. A composite index of state level family policies was created to conduct cross-sectional exploratory data analysis of female labor force participation. Additionally, a qualitative phenomenological study of solopreneurs provided a deeper understanding of the …

Tac-It An Affective Computing User Interface Design, Andrew Biron

Theses and Dissertations

TAC-IT affective computing user-interface design is an independent computer peripheral that is a tool to be utilized to obtain a user’s self-reported emotional state in real-time. Doctor Rosalind Picard first coined and used the term affective computing in her paper Affective Computing [Picard, R. (1995)]. Affective Computing is defined as the study and development of systems and devices that can recognize, interpret, process, and simulate human affects. It is an interdisciplinary field spanning computer science, psychology, and cognitive science. Since that time, areas of research have expanded exponentially, and areas of interest include how to trigger emotions in a test …

Investigation Of Reproductive Inhibition During Chronic Heat Stress In C. Elegans, Macy Makenna Nielander

Theses and Dissertations

Nearly all organisms experience heterogeneity in environmental conditions that can negatively impact fitness. A common adaptive strategy to reduce the impact of this stress is called facultative diapause, where development and reproduction are temporarily arrested to promote survival. Facultative diapause is characterized by three major criteria, the arrest must be (i) induced by an unfavorable environmental condition, (ii) reversible, and (iii) driven by genetically regulated mechanisms. The aim of this thesis was to determine whether chronic heat-induced inhibition of egg laying in Caenorhabditis elegans represents a novel form of facultative diapause. It was found that the inhibition of egg-laying is …

Using Nano-Ities For Detection Of Metal Ions In Complex Aqueous Solutions, Mohammed Muzammil Nishar Ahmed

Theses and Dissertations

Recent urbanization has induced a rapid increase in heavy metal exposure to humans. The exploitation of heavy metals for economic gains coupled with poor and economically infeasible recycling methods have led to poor management of heavy metal wastes, which has opened up several pathways for heavy metals to enter the human body. Such heavy metals target many vital organs, but the early symptoms are common and difficult to diagnose. Severe symptoms appear in later stages and are very expensive to treat and the damage caused to the health are not typically reversable in such later stages. To counter this issue, …

Adsorption Of Harmful Algal Bloom Toxins Using Biochar, Cadianne Chambers

Theses and Dissertations

Harmful Algal Bloom (HAB) events involve the proliferation of microalgae which discharge lethal toxins into fresh and brackish water bodies due to anthropogenic and climatic activities. Common examples of HABs include causative organisms such as Cyanobacteria, Pyrodinium bahamense and more. These genera are known to release potent toxins including Microcystins, and Saxitoxins, respectively, which can seriously endanger human and marine health. Ingestion of these toxins can result in several health problems, such as nausea, vomiting, gastroenteritis, skin irritation, liver damage, paralysis, and respiratory arrest. The Great Lakes, Gulf of Maine, Florida’s coasts, Lake Erie, and Lake Okeechobee have all been …

Dual-Channel Side Channel Attack: Improved Aes Key Decryption By Combining Power And Electromagnetic Side Channels With Convolutional Neural Networks, Sean P. O'Neill

Theses and Dissertations

This research introduces a novel DL approach for SCA that combines power consumption and EM signals to enhance encryption key deduction by leveraging a dual-channel CNN architecture. A new dataset, consisting of simultaneous power and EM signal collections during 128-bitAES encryption, was developed to train and evaluate the model’s effectiveness. The combined approach achieved an 88% reduction in traces needed, from 50 traces to 6, for encryption key classification, outperforming traditional methods such as random forest, DPA, DEMA,and individual side channel CNN models. These findings highlight the potential of integrating multiple side channels in SCA to improve performance without the …

Soil Biogeography And Community Assembly In Polar Regions, Jesse Jorna

Theses and Dissertations

The polar regions provide community ecologists with natural microcosms, that include reduced species diversity, steep environmental gradients and limited human perturbance. Soil biodiversity in the polar regions is poorly understood, and the driving forces behind community compositions can range in scale from glacial forces 20,000 years ago to the impact of a researcher's footstep. Here, we present the results of an investigation of the driving factors behind biodiversity in Arctic and Antarctic soils, with specific attention to the role of community assembly processes. We suggest a framework for investigating the effects of inter-bacterial community processes like facilitation and argue for …

Enhancing Muscle Health And Modulating Immune Response With Galectin-1 In Lgmd2b/R2 Models, Stefannia Esparza Pulido

Theses and Dissertations

Limb-Girdle Muscular Dystrophy Type 2B/R2 (LGMD2B/R2) is a type of dysferlinopathy, a progressive muscle-wasting disorder caused by mutations in the dysferlin gene. Hallmarks of the disease include chronic inflammation, impaired muscle regeneration, and eventual muscle degeneration. Currently, no FDA-approved therapies are available for dysferlinopathies. Recombinant human Galectin-1 (rHsGal-1) has shown multiple roles in maintaining skeletal muscle homeostasis, promoting muscle repair, enhancing myogenesis, and modulating inflammation by downregulating the NF-κB pathway. The ability of rHsGal-1 to modulate the immune response is particularly promising for therapeutic applications in patients with LGMD2B/R2. In this study, we evaluated the effects of rHsGal-1 in a …

Rare Sugars And Dairy Ingredients: A Synergistic Pairing For The Reduction Of Food Waste And Sugar Consumption, Melinda Michelle Moss

Theses and Dissertations

In the future, sustainability in the dairy industry will require creative and scale-able solutions for better utilization of the primary solid component in milk: lactose. One promising avenue of “up-cycling” underutilized lactose is converting it to valuable rare sugars. Rare sugars like D-tagatose and D-allulose are increasing in popularity extremely quickly. D-tagatose and D-allulose are hexose sugars, D-allulose being an epimer of D-fructose and D-tagatose an isomer of D-galactose. They have excellent functional properties in food systems with clean sweetness profiles similar to sucrose. Critically, they also have excellent nutritional characteristics, such as low caloric values and low glycemic indices. …

Ionic Nanomedicines And Biocompatible Hydrogel: Synthesis, Characterization And Application In Cancer And Drug Delivery, Mujeebat Bashiru

Theses and Dissertations

This dissertation is focused on the development of novel class of ionic materials (IMs), and ionic nanomedicines (INMs) for cancer treatment. These IMs are composed of two therapeutics combined via electrostatic attraction. The impact of various counterions on the drug’s cytotoxic and photophysical properties are unveiled. Moreover, the mechanism of internalization, enhanced toxicity of these materials is investigated by determining their cellular uptake, subcellular localization, and cell death mechanism. Furthermore, the in vivo bioimaging capabilities of the drugs is investigated to validate their potential for clinical relevance. The second part of this dissertation is focused on the synthesis of biocompatible …

Investigating Spatiotemporal Trends Using Precursory Signatures: Implications To Develop Short-Term Earthquake Forecasting Techniques In Sumatra-Andaman Region, Ramya Jeyaraman J

Theses and Dissertations

Earthquake forecasting is a challenging field due to Earth's heterogeneous nature. This research aims to develop a short-term earthquake forecasting model by analyzing spatiotemporal trends and precursory signatures in the Sumatra-Andaman region, known for its high seismic activity and tsunami risk. The study adopts an interdisciplinary approach, integrating solid earth tides (SET), micro shocks, and outgoing longwave radiation (OLR) to gain deeper insights into seismic nucleation processes. The research begins by using Singular Spectral Analysis (SSA) to identify potential seismically vulnerable areas through the analysis of irregularities in SET.

A spatiotemporal analysis of micro shocks is conducted to assess the …

Investigation On The Impact Of Loading Effect Of Fruit Juices On The Performance Of Pulsed Electric Field Generators, Devi S

Theses and Dissertations

Pulsed Electric Field (PEF) treatment is one of the efficient non-thermal food processing techniques which is being preferred as a replacement for thermal pasteurization methods. The effectiveness of PEF treatment was measured in terms of reduction in the microbial load in the food, extension of shelf life of the food and retention of nutritional properties of the food. The successful implementation of the PEF treatment depends upon various aspects such as design of pulse generator, parameters of pulse generator, shape and size of the treatment chamber and most importantly the characteristics of each food items. Design and fabrication of a …

Investigating Secondary Metabolites Of Streptomyces And Bacillus Bacteria That Inhibit Colony Growth Of Pathogenic Potato Fungi (Colletotrichum Coccodes, Pythium Ultimum), Jacob R. Henrie

Theses and Dissertations

Potato (Solanum tuberosum) is a crucial global food crop, yet its production is threatened by fungal pathogens such as Pythium ultimum and Colletotrichum coccodes, which cause significant yield losses and impact tuber quality. In this study, we investigated the antifungal properties of various Streptomyces and Bacillus isolates against these pathogens. The Streptomyces isolates, known for their prolific secondary metabolite production, were selected for their varying efficacy against these fungi. Despite the anticipated potential, Streptomyces isolates exhibited limited antifungal activity against Pythium ultimum and only moderate activity against Colletotrichum coccodes. In contrast, Bacillus isolates, particularly B1, demonstrated strong inhibition of C. …

Biodegradable Polymeric Microspheres For Magnetically Guided Drug Delivery To Tumors, Tyler Payson Green

Theses and Dissertations

This thesis investigates the feasibility of utilizing biodegradable polymeric microspheres loaded with the anticancer drug 5-fluorouracil (5FU) and superparamagnetic iron oxide nanoparticles (SPIONs) to magnetically deliver the cancer therapeutic 5FU to a target tumor in the human body. The primary method of material loading consisted of a w/o/w double emulsion mechanism which 1) loads and protects 5FU in the inner water phase consisting of distilled water and polyvinyl alcohol (PVA), 2) dispersed SPIONs in the biodegradable polymeric organic phase consisting of methylene chloride (MeCl2) for eventual magnetic transport, and 3) suspended these w/o emulsion droplets in an outer aqueous phase …

Biofabrication Of Tissue Constructs Using Protein-In-Polysaccharide Bioinks For Muscle Regeneration, Harshavardhan B

Theses and Dissertations

Myocardial infarction (MI) and volumetric muscle loss (VML) are debilitating conditions that severely affect patient quality of life and challenge healthcare systems, particularly in diagnosis and treatment. Conventional therapies often result in long recovery periods with limited functional restoration. Advances in regenerative medicine, especially extrusion-based 3D bioprinting, offer promising potential to improve the regeneration of complex tissues by precisely depositing cells and biomaterials layer-by-layer to create tailored tissue constructs.

A critical challenge in muscle tissue regeneration is developing protein-polysaccharide bioinks that closely mimic the native muscle tissue microenvironment. In this study, two protein-inpolysaccharide bioinks (alginate-fibrinogen and methylcellulose-gelatin-alginate) were developed to …