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Regeneration Of Electrospun Bioresorbable Vascular Grafts: A Phenomenon Associated With Vascular Graft Properties And Macrophage Phenotypes (M1/M2), Koyal Garg

Theses and Dissertations

Macrophages (MФ) and mast cells are important cell types in the context of tissue remodeling and regeneration. Mast cells participate in the early stages of wound healing and modulate the acute inflammatory responses to biomaterials. Mast cells can secrete a myriad of different cytokines by the process of degranulation; the process of regulated secretion in which preformed contents stored in their granules are rapidly released by exocytosis. Some of these cytokines such as IL-4, IL-13 and TNF-α can modulate the MФ phenotype. Macrophages (MΦ) are innate immune cells, crucial for tissue homeostasis, presentation of foreign and self-antigens following infection/injury, pathogen …

Novel Fluorous Hybrid Surface Modification Characterized By Wetting Dynamics, Morphology And Nanomechanics, Sithara Nair

Theses and Dissertations

The surface response of a polymer substrate to external stimuli such as initial wetting is controlled by the outermost molecular layer. Thus, changes on the nanoscale may be engaged to control macroscale wetting behavior. Our work has predominantly focused on surface modification of conventional polyurethane coatings (HMDI-BD-PTMO). Studies on network constrained phase separation and facile polydimethylsiloxane surface functionalization led to the discovery of a simpler one-step and more general approach to functional polymer surfaces that we have designated as “Bottle-Brush Nanoglass” (BB-NG) after the two principle components: (a) a polyoxetane soft block “spine” with side chain “A” bristles and triethoxysilyl …

Cholesterol Dyshomeostasis And Age Related Macular Degeneration, Bhanu Chandar Dasari

Theses and Dissertations

This dissertation work focused on retinal modifications that are relevant to Age-related macular degeneration (AMD) in cholesterol-fed rabbit model of Alzheimer's disease (AD), as AD and AMD share common features. It is unknown whether cholesterol-fed rabbit model of AD displays any AMD features in retina. Previous research showed 27-hydroxycholesterol (27-OHC) involvement in AD like pathology in organotypic hippocampal slices of rabbit brain and human SHSY-5Y neuroblastoma cells. The extent to which and the mechanisms by which 27-OHC may also cause pathological hallmarks related to AMD are not known. Various studies suggested estrogen's (E2) role in AMD development. 27-OHC is a …

Adult Enrichment Learners In St. Cloud, Minnesota: Motivational Reasons For Participation, Scott Wallner

Theses and Dissertations

The purpose of the study was to identify reasons and motivation of adult stakeholders that influence participation in adult community education enrichment classes in the St. Cloud Public School District, St. Cloud, Minnesota. The study also examined the perceptions about adult learners held by leaders, planners, and facilitators of these programs, and identified similarities and differences between perceptions by district staff and program participants.

Participants were 289 adult learners who had taken at least one adult enrichment course during the 2009 or 2010 calendar year. 284 participants completed the online Education Participation Scale-Form A, a 42-item survey developed by Boshier …

Effects Of A Large-Scale Wind Farm In The Prairie Pothole Region Of North And South Dakota On Survival And Habitat Use Of Breeding Female Mallards (Anas Platyrhynchos) And Blue-Winged Teal (A. Discors), Charles Tanner Gue

Theses and Dissertations

The Prairie Pothole Region (PPR) is the most important breeding habitat for North American ducks. However, much of the PPR is suitable for large-scale wind energy development and conflicts for waterfowl populations may occur if wind energy alters habitat in a way that reduces survival or productivity. Adult survival of breeding female ducks has a disproportionately large effect on population growth. Thus, populations might be particularly sensitive to increased mortality in this cohort because of direct collisions with wind turbines. Additionally, large home ranges are energetically taxing. If females avoid resources near wind turbines during breeding season activities, females may …

Genetic Relationships Of Wild Pigs (Sus Scrofa) In The United States: Geographic Origins And Genotypic Distribution Of The Species With Implications For Management, Blake Eugene Mccann

Theses and Dissertations

Wild pigs are a damaging invasive species with a long history in the United States. However, during the last 30 years wild pigs have drastically expanded their invasive range and are now present in 44 U.S. states. Though historic records provide insights regarding original introduction histories in areas where pigs are long-established, little is known regarding sources for new populations. To develop a better understanding of recent invasions, I utilized an array of molecular markers (mitochondrial DNA sequence, nuclear microsatellites, and nuclear single nucleotide polymorphisms) to evaluate both the evolutionary history of introduced pigs and gene flow between populations indicative …

Mining Aircraft Telemetry Data With Evolutionary Algorithms, Kirk Anders Ogaard

Theses and Dissertations

The Ganged Phased Array Radar - Risk Mitigation System (GPAR-RMS) was a

mobile ground-based sense-and-avoid system for Unmanned Aircraft System (UAS)

operations developed by the University of North Dakota. GPAR-RMS detected proximate

aircraft with various sensor systems, including a 2D radar and an Automatic Dependent

Surveillance - Broadcast (ADS-B) receiver. Information about those aircraft was then

displayed to UAS operators via visualization software developed by the University of

North Dakota. The Risk Mitigation (RM) subsystem for GPAR-RMS was designed to

estimate the current risk of midair collision, between the Unmanned Aircraft (UA) and a

General Aviation (GA) aircraft flying under …

Later Nineteenth Century Marriage And Divorce Law: The Changing Historiography Of The Law And British Women's History, Michael Beau Mishler

Theses and Dissertations

This thesis examines how the historiography of women's history and legal history with regard to later nineteenth century England have failed to interact in an appreciable way. In failing to do so a gap has gradually emerged between these two related but separate areas of historical inquiry. Women's and later gender history has tended to focus more on what women's lives were like and their roles both inside and outside the home. Legal historians and scholars in general have focused primarily on changes in marriage, divorce, and property law but they have failed to explain or show how legal reform …

Perceived Deployment Stressors And Well-Being Among Veterans Of Iraq And Afghanistan, Abigail Fuller

Theses and Dissertations

The current wars in Iraq and Afghanistan are unlike previous wars and present new challenges for service members post deployment. Currently, service members deployed to Iraq and Afghanistan are experiencing a number of stressors that may have implications during their post deployment adjustment (Kline et al., 2010; La Bash et al., 2009; McNulty, 2005). Few studies were found that examined the stressors of deployment and their relationship to the health and well-being of veterans upon return home. Therefore, a more complete understanding of the deployment experiences of Iraq and Afghanistan veterans is needed in order to have an adequate understanding …

Peer Mentoring And Empathy, Ronald Wayne Hochstatter

Theses and Dissertations

This study investigated the impacts of mentoring training, empathy training and sex difference on peer mentoring relationships. Mentoring training was conducted by the investigator and empathy training was provided by the University of North Dakota's Conflict Resolution Center. A total of 174 participants enrolled in an eight-week peer mentoring program. Participants were randomly selected and placed in four groups, no training, mentoring training, empathy training and both mentoring and empathy training. Mentors were asked to complete the Interpersonal Reactivity Index and the Prosocial Personality Battery. Protégés were asked to complete the Protégé Satisfaction Scale and the Relational Assessment Scale. Findings …

Analysis Of Yscf And Associated Homologs And The Presence Of An Inflammatory Molecular Pattern, William Andrew Roughead

Theses and Dissertations

Gram-negative pathogen, Yersinia pestis, expresses a type three secretion system to accomplish its virulence. The YscF needle protein provides a conduit of effectors from bacterium to eukaryote and is exposed to the host immune system. This thesis explores YscF and its associated needle homologs and the possibility of recognition as pathogen-associated molecular patterns (PAMP) by macrophage tissue culture. Interestingly, Salmonella homologs PrgI and SsaG are inflammatory suggesting conservation of the inflammatory pattern in the T3SS needle protein.

The Urotsa Bladder Cell Model For Heavy Metal Carcinogenesis: Characterization With Respect To The Role Of Beclin-1 And Sparc Expression, Jennifer Larson

Theses and Dissertations

The development of bladder cancer is known to have a strong association with environmental toxins. This laboratory employs the human Urotsa cell line model to explore the relationship between As+3 and Cd+2 exposure and the development of urothelial cancer.

The parental Urotsa cells and their As+3 and Cd+2 transformed counterparts have been used to define the mechanism of cell death (apoptosis and/or necrosis). A third mechanism of cell death, autophagy, has not yet been investigated. The hypothesis for the current study is that the autophagy pathway involving beclin-1plays a role in Urotsa cell death mechanisms. A combination of real time …

Intimate Relationships Of Adults Raised In Same-Sex Families, Thomas Roskos

Theses and Dissertations

The purpose of this study was to explore the close relationship experiences of adults raised by sexual minority parents. The study used a Grounded Theory (GT) method. Seven individuals that were either born to sexual minority parents or found out their parents were sexual minorities later in life participated in this present study. Each participant completed an over the phone, semi-structured interview. Specifically, questions were asked regarding their family history, relationships with their parents, peers, romantic partners, and others, their experiences with heterosexism, their experiences coming out, and their perspectives on sexuality and gender.

In accordance with GT, the data …

Synthesis, Characterization, And Self-Assembly Of Bi-Carbamates And Tri-Carbamates, Rajiv Kumar Singh

Theses and Dissertations

Recent decades have witnessed the discovery of a wide variety of nanomaterials. These nanomaterials vary from zero-dimensional nanodots to three-dimensional nanoflowers. However the properties of these nanomaterials have not been fully explored. A majority of the nonmaterials discovered so far are inorganic. There is little information about nanomaterials which are entirely based on organic compounds. Therefore this thesis focuses on the synthesis of two-dimensional nanosheets based on organic frameworks.

In this study, a number of two-armed and three-armed carboxamides and carbamates have been synthesized, characterized and self-assembly process was studied. The self-assembly of these amides and carbamates were studied in …

Professional Behaviors, Sense Of Belonging, And Professional Socialization Of Early Career Clinical Laboratory Scientists, Janna Marie Schill

Theses and Dissertations

Professional socialization is a process that individuals experience as members of a profession and consists of the knowledge, attitudes, and experiences that influence and shape their professional identity. The process of professional socialization has not been studied in the clinical laboratory science profession. Clinical laboratory science is an allied health profession that is faced by a workforce shortage that has been caused by a decrease in new graduates, decreased retention of qualified professionals, and increased retirements. Other allied health professions such as nursing, athletic training, and pharmacy have studied professional socialization as a way to identify factors that may influence …

Role Of Gravin In Endothelial Wound Healing, Bhaskar Roy

Theses and Dissertations

Gravin (AKAP12), a 300 kDa AKAP, is widely distributed in cells and binds to several signaling molecules including PKA-RII subunits, PKCα, PDE4, β2 adrenoreceptor and actin cytoskeleton. Initial characterization of gravin has revealed that this protein is undetectable in endothelia in vivo but expressed at the periphery of cultured endothelial cell (EC). Although the precise role of gravin in human ECs is not yet known, the cortical distribution of gravin in EC suggests that it may be involved in cAMP regulation by targeting PKA and PDE4 to plasma membrane. Several studies indicate that localization of PKA activity by A-kinase anchoring …

Experiences Of Female Faculty With Maternity Leave At Four-Year Universities In An Upper Midwest State, Audra Dawn Myerchin

Theses and Dissertations

The purpose of this mixed methods study was to explore female faculty members' experiences with maternity leave, while working as a career academic in higher education. Participants consisted of women currently employed in an upper Midwest state at six four-year institutions. An online survey was completed by 121 women, and 30 of these women also participated in face-to-face or telephone interviews. Survey participants completed both open and closed-ended questions. Face-to-face interviews were audio recorded and transcribed, then analyzed for codes, categories, and themes.

Four themes emerged from the analysis of the data: there was a lack of information on maternity …

Desire And Influence: Male Self-Realization And Film Progression Due To The Influential Women In The Films Of Wes Anderson, Erin Ann Meiers

Theses and Dissertations

My thesis is an analysis arguing that the roles of women in three of Wes Anderson's films, Rushmore (1996), The Royal Tenenbaums (2001), and The Life Aquatic(2004), are vital in influencing the men that surround them to come to a self-realization and in the progression of the film as each character finds closure, despite critic's claims. Critics Jesse Mayshark and Greg Carlson examine Anderson's films to be basically about men stuck in arrested development that as they rival each other they grow and mature, and discount the roles of women as simply a side note. I build upon their analysis …

Multiphysics Modeling And Simulation Process To Develop Thin Piezoelectric Film Sensors To Measure The Vibration Of Structures With Complex Shapes And Boundary Conditions., Md. Razib Rahim

Theses and Dissertations

Piezoelectricity was discovered in 1880 by Jacques and Pierre Curie. Its application has since been extended to actuators and sensors, widely used in industry, automotive, and aerospace applications. The last two decades have seen intensive research in piezoelectric theory in an effort to effectively capture and control the distinctive coupling of electricity and elasticity. However, due to the complexity of the theory involved, finite element and numerical methods are often used in the process. Limited analytical exact solutions are also found in literature. The objective of this work is to devise a multiphysics modeling and simulation process to develop thin …

Sex Determination And Differentiation In The Common Snapping Turtle - A Reptile With Temperature-Dependent Sex Determination, Anthony Loren Schroeder

Theses and Dissertations

Temperature-dependent sex determination (TSD) was first discovered in an African lizard over 40 years ago. TSD has since been shown to be exhibited by many vertebrates including some fish and amphibians, numerous lizards, turtles, and all crocodilians studied to date. Although numerous questions surround TSD, a major question focuses on understanding the genetic, physiological and molecular mechanisms underlying this process. However, the molecular mechanisms underlying TSD are not well known and the gene(s) that transduces a signal for ovary or testis development is not known in any species. Furthermore, it is well established that sex steroid hormones, androgens and estrogens, …

Theoretical Circular Dichroism Of Lysozyme, Myoglobin And Collagen And Experimental Circular Dichroism Of Myoglobin And Pea Lectin, Boris Anyangwa Sango

Theses and Dissertations

The need to understand protein structure and interaction is ever-growing and this has led to scientist and investigators utilizing numerous different techniques in order to obtain substantial insights and explanation to these structures and their interactions. Circular dichroism (CD), which is one of these techniques, is a powerful structural biology technique used to study protein and nucleic acid structures and their dynamics. This technique is important because it identifies the secondary, tertiary and even quaternary structures in proteins and can be used to study folding patterns in proteins. Theoretical and experimental methods are used to better understand and teach the …

Roles Of Ccaat/Enhancer-Binding Protein [Beta] And [-Delta] In Immunoglobulin G Immune Complex-Induced Inflammation, Chunguang Yan

Theses and Dissertations

CCAAT/enhancer-binding protein (C/EBP) &beta and C/EBP&delta are known to participate in the regulation of many genes associated with inflammation. However, little is known about the activation and function of C/EBP&beta and -&delta in inflammatory responses elicited by Fc&gamma receptor (Fc&gammaR) activation. Here I showed that C/EBP&beta and -&delta activation were induced in immunoglobulin G immune complex (IgG IC)-treated macrophages by using gel shift assays. The increased expression of C/EBP&beta and -&delta occurred at both mRNA and protein levels. Furthermore, induction of C/EBP&beta and -&delta was mediated, to a large extent, by activating Fc&gammaRs. Using small interfering RNA (siRNA)-mediated knockdown as …

Factors Impacting Clinical And Counseling Psychology Students Conducting Research With Lgbt Populations, Daniel Walinsky

Theses and Dissertations

Although a large body of literature exists on training clinical and counseling psychology researchers, scant empirical work has been published that looks at the unique needs of students working with lesbian, gay, bisexual, and transgender (LGBT) populations. This study explores researcher development experiences of those students.

Two hypotheses are tested with a sample of 59 doctoral students in APA-accredited clinical and counseling psychology program. The first hypothesis posits that Exploration and Commitment Factors of the Measure of Sexual Identity Exploration and Commitment (MoSIEC) will account for variance on the Research Outcome Expectations Questionnaire (ROEQ) beyond that accounted for by social …

Modeling Intermittent And Perennial Headwater Stream Origins In North Carolina., Kevin Macleod

Theses and Dissertations

Low-order headwater streams, a link between the upland landscape and larger streams within a watershed, affect watershed hydrology. Yet current widely available maps inadequately depict headwater streams, underestimating channel length or omitting channels entirely. Intermittent and perennial headwater streams each provide unique habitat. Until recently, it has not been possible to accurately delineate these streams or predict channel flow durations without fieldwork. Recent advances in remote sensing may allow more accurate headwater stream mapping.

Methods for mapping intermittent and perennial headwater streams with a GIS-based modeling approach coupled with field verification were adapted, tested, and evaluated. Four models were developed …

Electroluminescence Studies On Longwavelength Indium Arsenide Quantum Dot Microcavities Grown On Gallium Arsenide, John C. Ramsey

Theses and Dissertations

A comprehensive study of the electroluminescence of four GaAs/AlGaAs microcavity devices with InAs/GaInAs quantum dot active regions emitting near 1.3 µm was conducted. The four molecular beam epitaxial grown samples with AlAs oxide aperture confinement layers were fabricated, characterized, and optically modeled. Optical power transmission of the samples was modeled using Matlab and compared with measured transmission data. Resonant cavity light emitting diodes (RCLEDs) and three vertical cavity surface emitting laser (VCSEL) samples were fabricated and electro-optically characterized over a range of injection currents and temperatures. Devices achieved continuous wave room temperature lasing at 1.28 µm with an output power …

A Method For Measuring Programmatic Dependency And Interdependency Between Dod Acquisition Programs, Matthew B. Christensen

Theses and Dissertations

There is currently no accepted method for quantitative measurement of acquisition program interdependency. While some methods exist for measuring dependency or interdependency at the component or system level, these methods do not translate well to program interdependency measurement. The objective of this thesis is to provide a model for measuring acquisition program interdependency accurately and quantitatively. The model presented in this thesis uses four Interdependency Factors to identify dependency relationships between programs. Specific Interdependency Levels are then used to measure the strengths of those dependencies. The model also accounts for measurement of dependencies upon programs that are not directly connected, …

Development Of Photographic Dynamic Measurements Applicable To Evaluation Of Flapping Wing Micro Air Vehicles, Jeremy C. Murray

Theses and Dissertations

Developments in the area of flapping wing micro air vehicles (FWMAVs) of a small size and with limited range. This has lead to a great deal of interest in biomimetric designs based on flapping wing flyers, including the North American Hawkmoth (Manduca Sexta). By utilizing high speed photography and photogrammetry the dynamic flapping of the wing can be characterized for comparison with mathematical models, namely computational fluid dynamics (CFD) and finite element analysis (FEA).To successfully utilize high speed image capture, a method of successfully digitizing many data points in a short period of time needs to be developed. The effort …

Design And Synthesis Of Cationic Steroid Antimicrobial Compounds, Synthesis Of Glycolipids Recognized By Natural Killer T Cells And Development Of Tlr-1, Tlr-6 Heterodimer Binders And Studies Of Their Immunology Activities, Yanshu Feng

Theses and Dissertations

Cationic steroid antimicrobial agents (CSAs) are a family of bile acid derivatives. These compounds are amphiphilic and mimic endogenous antimicrobial peptides. The antimicrobial activities of CSA-13 have been investigated and due to portent bactericidal activities and low toxicity, a large amount of CSA-13 is demanded for clinic trails and other antimicrobial applications. During our studies, we optimized the synthetic route of CSA-13, so that it can be prepared at the kilogram, even in tons scale. We investigated three routes and one of them is suitable for industry, because only recrystallization is needed in the synthesis. Natural killer T cells (NKT …

Characterization Of The Role Nuclear Bmp2 (Nbmp2) Plays In Regulating Gene Expression, Fialka Grigorova

Theses and Dissertations

The nBmp2 protein was first identified in a DNA affinity chromatography/mass spectrometry screen designed to detect proteins that interact with a cartilage-specific enhancer element (called D/E) from the type XI collagen gene Col11a2. The transcription factor SOX9, a protein from the Sox (SRY-related HMG box) family, binds to and activates gene expression from this enhancer. nBmp2 has no transcriptional activity of its own on this enhancer, but when co-transfected with SOX9 it increases SOX9's activation of D/E nearly 2-fold. SOX9 also activates cartilage-specific enhancer elements from the Col2a1, Col27a1, and Col9a1 genes. The purpose of this project …

Influence Of Weak Interactions On Supramolecular Binding: Characterization Of Cucurbituril Complexes With Alkylmonoammonium Ions Using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, Ruijan Shi

Theses and Dissertations

This thesis focuses on using mass spectrometry-based techniques for characterizing the structure and behavior of cucurbituril complexes in the gas phase. Both solvent and counter ion effects can be completely eliminated in the gas phase and the intrinsic interactions in the system are directly shown in the experimental results. Chapter 1 introduces the structures, properties and host-guest chemistry of cucurbituril, and FTICR mass spectrometry including instrumentation, performance and working principles. Two mass spectrometry-based methods for supramolecular characterization, sustained off-resonance irradiation collision induced dissociation (SORI CID) and ion molecule equilibrium measurements, are also discussed in this chapter. Chapter 2 characterizes the …