Digital Commons Network^™

Evaluating Climate Change Effects In Two Contrasting Reservoirs Using Two-Dimensional Water Quality And Hydrodynamic Models, Oliver Obregon

Theses and Dissertations

I analyzed and compared impacts from global climate change (GCC) and land use change to Deer Creek (United States) a temperate reservoir and Aguamilpa (Mexico), a tropical reservoir by using calibrated CE-QUAL-W2 (W2) water quality and hydrodynamic models based on field data over an extended time period. I evaluated and compared the sensitivity to predicted GCC and land use changes. I individually evaluated changes to air temperature (TAIR), inflow rates (Q), and nutrient loads (PO4-P and NO3-NO2-N) followed by analysis of worst case scenarios. I developed analysis methods using indexes to represent the total …

A Methodology For Strategically Designing Physical Products That Are Naturally Resistant To Reverse Engineering, Stephen P. Harston

Theses and Dissertations

Reverse engineering - defined as extracting information about a product from the product itself - is a design tactic commonly used in industry from competitive benchmarking to product imitation. While reverse engineering is a legitimate practice - as long as the product was legally obtained - innovative products are often reverse engineered at the expense of the pioneering company. However, by designing products with built-in barriers to reverse engineering, competitors are no longer able to effectively extract critical information from the product of interest. Enabling the quantification of barriers to reverse engineering, this dissertation presents a set of metrics and …

Visual Attention, Color Processing And Physiological Measure Differences In Males And Females With Substance Abuse And Opiate Addiction, Jo Ann Petrie

Theses and Dissertations

A biological marker of the addictive state would be a major breakthrough in objectively assessing the efficacy of treatment outcomes. Given its role in the mesolimbic system and drug reward, most biological marker studies for addiction focus on measures related to dopamine (DA). Dopamine is also implicated in some disorders of visual attention and plays a modulatory role in the processing of color in the retinal DA pathway. For example, visual processing in the retina has been shown to co-vary with DA levels during cocaine withdrawal. In this electroencephalographic (EEG) study, we studied event related potentials (ERPs) and reaction time …

Drug Delivery Strategies Using Light Sensitive Molecules, Martin Dcona

Theses and Dissertations

Cancer remains one of the most dreaded diseases due to inevitable suffering and possible fatality. Only cardiac disease has caused more deaths than cancer. Present day cancer treatment involves radiation, surgery or chemotherapy. In chemotherapy, an anti-tumoral drug is used to treat the tumor either by killing or stalling the growth of the tumor cells. In certain types of cancer, for e.g. metastatic breast cancer, the first line of therapy is often chemotherapy. But the inability of current clinically approved drugs to selectively target tumor cells, ultimately results in side effects. To reduce these side effects, prodrug therapies have been …

An Investigation Of Current Practice In Management Rotations In Dietetic Internships, Emily K. Vaterlaus

Theses and Dissertations

Background: Leadership and management skills are critical to moving the dietetics profession forward; acquisition of those skills begins in the Dietetic Internship (DI). This study examined DI program components related to higher mean scores on the Foodservice and Management domain of the Registration Examination for Dietitians and compared Program Director and Preceptor perceptions of management rotation structure.

Methods: All 242 DI Program Directors (excluding sponsoring institution) received a 47-item electronic questionnaire. Directors provided contact information for up to three DI management rotation primary preceptors. Preceptors received a 35-item electronic questionnaire regarding their perception of interns' management skill development. …

Power By Possession: Cuban-American Types And Collecting In The Agüero Sisters, Ashley Noelle Walton

Theses and Dissertations

Although many other ethnic and cultural studies have moved beyond essentialist labels and categories, Cuban-American studies persists in categorizing the people belonging to the cultural group in terms of how much time they have spent in Cuba, thus creating a hierarchy of "authentic" Cuban-ness. Isabel Alvarez Borland gives a comprehensive overview of Cuban-American literary categories, and through her description we can see how these categories may lend themselves to stratification. These categories include: the first generation, the second generation, the one-and-a-halfer, and the ethnic writer. To say that one generation of Cuban exiles is more or less authentically Cuban discounts …

At Second Glance: Retroactive Continuity In Junot Díaz’S The Brief Wondrous Life Of Oscar Wao, Stephen Clancy Clawson

Theses and Dissertations

This work explores Junot Díaz’s incorporation of nerd culture into his novel, The Brief Wondrous Life of Oscar Wao, and that move's larger impact on the genre of trauma narratives. By using allusions to nerd texts such as The Lord of the Rings to structure his depiction of the brutal reign of Dominican dictator Rafael Leónidas Trujillo Molina, Díaz effectively rewrites Dominican history, creating a retroactive continuity of fantasy. Retroactive continuity, or retcon, is a little-discussed interpretive strategy of the nerd community with striking parallels to Lacanian notions of fantasy. A reading of Díaz's retcon ultimately casts doubt on …

Phylogenetics Of Thysanoptera (Insecta: Paraneoptera), Rebecca S. Buckman

Theses and Dissertations

The order Thysanoptera (Insecta: Paraneoptera), commonly known as thrips, includes organisms that exhibit a wide range of social and feeding behaviors that are of particular interest in evolutionary studies. These studies within thrips have been inhibited by the lack of knowledge of thrips relationships. The recognized classification scheme strives to reflect evolutionary relationships and is based upon morphology. Molecular data is next as morphology alone is not enough to resolve relationships. Few molecular studies have been conducted and all were limited in their taxon sampling and genetic sampling. To provide a foundation of future evolutionary studies, the objectives of this …

The Poetics Of A Dominican Holocaust And The Aesthetics Of Witnessing, Andrew Mark Merrill

Theses and Dissertations

This study examines Julia Alvarez's best-known works, García Girls and In the Time of the Butterflies, to explore the intertextuality within Dominican-American fiction through the vocabulary and methodology of trauma studies and witnessing. Alvarez's work indicates that traditional academic discourse about witnessing often translates trauma survivors into tourists by legally dispossessing them from the witnesses they could provide as they seek to assign blame and pass judgment on the source of their traumatic experience. This process of exclusion threatens to hinder the ability of Dominican-Americans to work through their shared, traumatic experience with the Trujillo regime. Furthermore, this study …

Change In Group Therapy: A Grounded Theory Inquiry Into Group And Interpersonal Patterns In A Community Sample, Rebecca R. Canate

Theses and Dissertations

This qualitative study investigated the group process and impact of pre-group feedback information on individuals in an eight-week therapy group. The feedback information was based on group members' results on the Structural Analysis of Social Behavior (SASB) Intrex questionnaire given before the group began. The Intrex is based on Interpersonal Reconstructive Therapy (IRT) theory, which is a combination of interpersonal psychoanalysis, attachment theory, operant conditioning, and studies of imitative learning, and has been utilized primarily in an individual treatment format. Because only a limited number of treatment strategies have utilized IRT theory in the group treatment setting, and because group …

Novel Phosducin-Like Protein Binding Partners: Exploring Chaperone And Tumor Suppressor Protein Interactions, Amy Jetaun Gray

Theses and Dissertations

Many proteins cannot fold into their native state without the assistance of one or more molecular chaperones. Chaperonins are an essential class of chaperones that provide an isolated chamber for proteins to fold. CCT, a group II chaperonin found in eukaryotes assists in the folding of actins, tubulins, and many other cellular proteins. PhLP1 is a member of the phosducin protein family that assists CCT in the folding of Gβ and its subsequent assembly with Gγ. However, previous studies have not addressed the scope of PhLP1 and CCT-mediated Gβγ; assembly. The data presented in Chapter 2 shows that PhLP1 plays …

Family Implicit Rules, Child Self Regulation, And Observed Child Emotional Responsiveness To Parents, Mark J. Mauzy

Theses and Dissertations

The purpose of this study was to examine how implicit family process rules are related to observed child emotional responsiveness with child self regulation as a possible mediating variable. Data from Wave 1 of the Flourishing Families project was used and included 337 two parent families and a target child between the ages of 10 and 13. Mother and father perception of family implicit rules were used to measure family implicit rules; child and mother report of the child's self regulation were used to measure self regulation, and child's emotional responsiveness to mother and father were taken from coding data. …

Follicular Dendritic Cells, Human Immunodeficency Virus Type 1, And Alpha 1 Antitrypsin, Xueyuan Zhou

Theses and Dissertations

HIV/AIDS is raging and causing millions of deaths around the world. The major challenge in treating HIV/AIDS is the establishment of HIV reservoirs where the viruse escapes both drug and immune system attempts at eradication. Throughout the course of HIV/AIDS, productive HIV infection occurs primarily in the lymphoid follicles or germinal centers (GC) surrounding follicular dendritic cells (FDC). In the GCs, FDCs trap and maintain infectious HIV for years and provide these infectious viruses to the host cells. FDCs also attract B and T cells into the GCs and increase the ability of CD4+ T cells to be infected. Additionally, …

Plant-Soil Feedbacks And Subalpine Fir Facilitation In Aspen-Conifer Forests, Joshua R. Buck

Theses and Dissertations

This thesis includes two studies. The first study examined changes in soil characteristics as a result of prolonged conifer dominance in successional aspen-conifer forests. Changing disturbance patterns in aspen-conifer forests appear to be altering successional dynamics that favors conifer expansion in aspen forests. The primary objective of this paper was to identify how increasing conifer dominance that develops in later successional stages alters forest soil characteristics. Soil measurements were collected along a stand composition gradient: aspen dominated, aspen-conifer mix, conifer dominated and open meadow, which includes the range of conditions that exists through the stages of secondary succession in aspen-conifer …

An Object-Based Image Analysis Of Treated And Untreated Pinyon And Juniper Woodlands Across The Great Basin, April Hulet

Theses and Dissertations

Land managers need to rapidly assess vegetation composition and bare ground to effectively evaluate, manage, and restore shrub steppe communities that have been encroached by pinyon and juniper (P-J) trees. A major part of this process is assessing where to apply mechanical and prescribed fire treatments to reduce fuel loads and maintain or restore sagebrush steppe rangelands. Geospatial technologies, particularly remote sensing, offers an efficient option to assess rangelands across multiple spatial scales while reducing the need for ground-based sampling measurements. High-spatial resolution color-infrared imagery (0.06-m pixels) was acquired for sagebrush steppe communities invaded by P-J trees at five sites …

Cheatgrass (Bromus Tectorum), Native Grasses, And Small Mammals In The Great Basin: A Test Of The Apparent Competition Hypothesis Facilitated By A Novel Method Of Decanting Seeds From A Flotation Solution, Jacob Elias Lucero

Theses and Dissertations

The effect of shared enemies between invasive and native species has been argued to facilitate biological invasions (i.e., the apparent competition hypothesis or ACH). This study investigated a previously untested possibility: whether granivorous small mammals facilitate cheatgrass (Bromus tectorum) invasion by driving food-mediated apparent competition between cheatgrass and native grasses. Specifically, we tested three predictions that must be true if such apparent competition occurs. First, cheatgrass invasion augments total seeds available to granivorous small mammals. Second, density of granivorous small mammals increases in response to increased seed availability (simulated with experimental additions of cheatgrass seeds). Third, granivorous small …

Optical And Mass Spectrometric Studies Of A Helium Dielectric-Barrier Atmospheric-Pressure Plasma Jet Used As An Ambient Desorption Ionization Source, Matthew Spencer Heywood

Theses and Dissertations

Recently there has been a surge in the field of mass spectrometry centered around the concept of rapid analysis of target analytes with minimal or no sample preparation. The target analyte undergoes desorption from its surface of origin and is subsequently ionized under ambient conditions. The technique is termed ambient desorption/ionization mass spectrometry (ADI-MS). Since the introduction of ADI-MS in 2004, there has been an explosion of research based around the development of novel ambient desorption/ionization (ADI) sources with the capability of desorbing and ionizing a variety of target analytes from various sampling surfaces. One type of ADI source uses …

Low-Photoluminescence Hollow Waveguide Platforms For High-Sensitivity Integrated Optical Sensors, Yue Zhao

Theses and Dissertations

This dissertation presents research on the fabrication of optofluidic sensor platforms, which consist of integrated hollow waveguides and solid waveguides. Antiresonant reflecting optical waveguides (ARROWs) filled with liquids or gases, can be used for high-sensitivity sensing in applications of biotechnology, chemical synthesis, and analytical chemistry. The fabrication method developed for integrated ARROW sensing platforms utilizes standard microfabrication processes and materials. Dielectric cladding layers are deposited on a silicon wafer using plasma-enhanced chemical vapor deposition (PECVD) or sputtering. A sacrificial material is then patterned over the bottom cladding layers by photolithography. Additional dielectric layers are deposited around the core, forming the …

Feature Construction Using Evolution-Constructed Features For General Object Recognition, Kirt D. Lillywhite

Theses and Dissertations

Object recognition is a well studied but extremely challenging field. Human detection is an especially important part of object recognition as it has played a role in machine and human interaction, biometrics, unmanned vehicles, as well as tracking and surveillance. We first present a hardware implementation of the successful Histograms of Oriented Gradients (HOG) method for human detection. The implementation significantly speeds up the method achieving 38 frames a second on VGA video while testing 11,160 sliding windows per frame. The accuracy remains comparable to the CPU implementation. Analysis of the HOG method and other popular object recognition methods led …

Adolescent Girls' Experience Of Binge And Loss Of Control Eating, Allison Palmberg

Theses and Dissertations

The current investigation used qualitative methodology to examine adolescent girls’ perceptions of control over their eating, as well as triggers, and consequences of binge and related eating behaviors. Focus groups were completed with 19 adolescent girls (aged 13-17, 58% African American, 41% White) who endorsed the behaviors. Responses to focus group questions were qualitatively analyzed using a grounded theory approach and constant comparison coding. Results reflected a fundamental lack of awareness of the loss of control (LOC) eating behaviors. Yet, the data did reflect a central theme of the need to affirm independence and autonomy through eating behaviors via three …

Family Structure And Family Instability: Evaluating Their Influence On Adult Outcomes, Hsin-Yao Chiu

Theses and Dissertations

Family structure is a widely used independent variable. However, in recent years researchers have questioned its predicting power. They also strive to improve its use. This study drew on Holman's (2001) theoretical model and examined the association between the family structure independent variables (Family Structure, Family Instability, and Parental Romantic Partners) and the outcome variables (Coming to Terms, Self-esteem, Maturity, and Depression). The analysis was conducted with a sample of 3,705 individuals (2,316 females and 1,389 males) randomly selected from the entire population that completed the Relationship Evaluation Questionnaire (RELATE). Results showed that Family Instability was a stronger predictor than …

Partial Characterization Of The Antimicrobial Activity Of Ccl28, Bin Liu

Theses and Dissertations

This research focuses on the antimicrobial activity of the mouse chemokine CCL28. In addition to their well characterized chemotactic activity, many chemokines have been shown to be antimicrobial in vitro, including the mucosally expressed chemokine CCL28. I have investigated the primary sequence features required for antimicrobial activity, salt sensitive nature of killing/binding mechanism, and in vivo microbial interactions of CCL28. Through the use of protein mutation and expression techniques, I have shown that the holoprotein (108 amino acids) is necessary for full antimicrobial activity of CCL28. Furthermore, the C terminal region of CCL28 is essential for microbial killing as an …

Elevating Endogenous Cannabinoids Reduces Opioid Withdrawal In Mice, Divya Ramesh

Theses and Dissertations

Delta9-tetrahydrocannbinol (THC), the primary active constituent of Cannabis sativa, has long been known to reduce opioid withdrawal symptoms. Although THC produces most of its pharmacological actions through the activation of CB1 and CB2 cannabinoid receptors, the role these receptors play in reducing opioid withdrawal symptoms remains unknown. The endogenous cannabinoids, N-arachidonoylethanolamine (anandamide; AEA) and 2-arachidonylglycerol (2-AG), activate both cannabinoid receptors, but are rapidly metabolized by fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL), respectively. The objective of this dissertation was to test whether increasing AEA or 2-AG, via inhibition of their respective hydrolytic enzymes, reduces morphine withdrawal symptoms in …

Blood Glucose Monitoring And Metabolic Control In Youth With Type 1 Diabetes: Relation To Disease Care, Adrienne Borschuk

Theses and Dissertations

Better disease care behaviors in youth with type 1 diabetes (T1D) are strongly related to better metabolic control (HbA1c). However, HbA1c results are only available, on average, every three months, and may not accurately capture intricacies of blood glucose fluctuations. Youth then must rely on blood glucose levels obtained throughout the day to determine which disease care behaviors to perform to maintain optimal metabolic control. Youth may have difficulty performing these disease care behaviors properly or consistently, which makes parental monitoring a crucial aspect of the diabetes regimen. Additionally, youth who experience frequent or severe hypoglycemia may develop a fear …

Comparison Of Steriplex™ Hc And Sodium Hypochlorite Cytotoxicity On Primary Human Gingival Fibroblasts, Jesse Harris

Theses and Dissertations

This study examined the cytotoxic effects of STERIPLEX™ HC (sBioMed, Orem, UT) and sodium hypochlorite (NaOCl) on human fibroblast cells in vitro. Fibroblasts exposed to various concentrations of NaOCl or STERIPLEX™ HC were visualized via light microscopy. Dilutions of either NaOCl or STERIPLEX™ HC that did not appear to disrupt the integrity of the cells were recorded for further analysis. Cells were then cultured and grown to confluence in five separate plates. A void was created down the middle of each plate. If the cells were viable, cellular confluence was seen. If nonviable, confluence of the cells did not occur. …

The Antimicrobial Effect Of Steriplex Hc In Comparison With Sodium Hypochlorite On Enterococcus Faecalis, Jonathan Coudron

Theses and Dissertations

The study objective was to compare the antimicrobial activity of STERIPLEX™ HC with 5.25% sodium hypochlorite (NaOCl) at different dilutions (50%, 25%, 10%, 1%, 0.1%) and different time intervals (1, 3, 5 minutes) on Enterococcus faecalis. All data was analyzed using an ANOVA. The 50%, 25%, and 10% dilutions of both disinfectants reduced the colony forming unit (CFU) count to below the limit of detection (50 CFU/ml) after one minute. The 1% dilutions at each of the time intervals show NaOCl was significantly more effective than STERIPLEX™ HC (all Ps < .0001) in reducing the CFU/ml count. The 0.1% dilutions of NaOCl and STERIPLEX™ HC at 1 minute, were not different (P = 0.7808), while at 3 minutes and 5 minutes NaOCl was significantly more effective (P = 0.0098 and P < .0001, respectively).

Dynamics Of Substrate Interactions In Trna (M1g37) Methyltransferase: Implications For Drug Discovery, Maria Kiouppis Palesis

Theses and Dissertations

The bacterial enzyme t-RNA (m1G37) methyltransferase (TrmD) is an ideal anti-microbial drug target since it is found in all eubacteria, serves an essential role during protein synthesis, and shares very little sequence or structural homology with its eukaryotic counterpart, Trm5. TrmD, a homodimeric protein, methylates the G37 nucleotide of tRNA using S-adenosyl-L-methionine (SAM) as the methyl donor and thus enables proper codon-anticodon alignment during translation. The two deeply buried binding sites for SAM seen in X-ray crystallography suggest that significant conformational changes must occur for substrate binding and catalytic turnover. Results from molecular dynamics simulations implicate a flexible loop region …

Humanized Mice As A Model To Study Human Viral Pathogenesis And Novel Antiviral Drugs, Freddy Mauricio Sanchez Tumbaco

Theses and Dissertations

Animal models have greatly contributed to the understanding of different aspects of human biology, as well as a variety of human-related pathogens and diseases. In order to study them, humanized mice susceptible to pathogens that replicate in human immune cells have been developed (e.g., humanized Rag2-/-γc-/- mice). These animals are engrafted with human hematopoietic stem cells (HSCs), resulting in the de novo development and maturation of the major functional components of the human adaptive immune system and the production of a variety of human cell types. Primary and secondary lymphoid organs in the mouse are populated with human cells, and …

A Parametric Investigation Of Pattern Separation Processes In The Medial Temporal Lobe, Sarah E. Motley

Theses and Dissertations

The hippocampus is thought to be involved in memory formation and consolidation, with computational models proposing the process of pattern separation as a means for encoding overlapping memories. Previous research has used semantically related targets and lures to investigate hippocampal responses to mnemonic interference. Here, we attempted to define the response function of the hippocampus and its inputs during pattern separation by parametrically varying target-lure similarity in a continuous recognition task. We also investigated the effect of task demands (intentional versus incidental encoding) on pattern separation processes. We collected functional magnetic resonance imaging (fMRI) data while participants were shown a …

Spatial Distribution And Modulation Of Nitric Oxide Synthase In A Hypertensive Rat Model, Andrew Yannaccone

Theses and Dissertations

There are gaps in the fundamental understanding of the expression of nitric oxide synthases (NOS) in the microvasculature. We examined co-localization of NOS1 (nNOS), NOS2 (iNOS) and NOS3 (eNOS) in the spinotrapezius muscle of young adult male Wistar-Kyoto (WKY) and Spontaneously Hypertensive (SHR) rats according to fiber type using immunohistochemistry and brightfield microscopy. Data regarding fiber distribution, population and morphology data were collected. Alkaline phosphatase staining was used to determine capillary density and average number of capillaries around a fiber. Gel electrophoresis and Western blot techniques were used to compare myosin heavy chain (MHC) protein expression with fiber type population …