Digital Commons Network^™

Early Adolescent Nicotine Exposure Has Long-Lasting Effects On Cocaine-Induced Behaviors In Mice, Mai Alajaji

Theses and Dissertations

Nicotine is one of the most commonly used drugs among adolescent populations. Given the fact that adolescence is a unique developmental stage, during which nicotine has long-term effects on future drug-taking behavior, it is essential to understand how early exposure to nicotine during adolescence may affect the abuse liability of other drugs. We hypothesize that repeated exposure to low doses of nicotine in adolescence induce age-specific enhancement of the rewarding effects of several drugs of abuse in the conditioned place preference (CPP) test. Furthermore, we predict that these changes in behavioral responses are mediated by nicotine-induced brain region-specific increases in …

Kinetics And Mechanisms Of Accumulation For Liposomal Ciprofloxacin Into Rat Alveolar Macrophages, Sayeed Mossadeq

Theses and Dissertations

The kinetics and mechanism of accumulation for liposomal ciprofloxacin (Lipo-CPFX) into the rat alveolar macrophage NR8383 cells were studied in vitro, in comparison to unformulated ciprofloxacin (CPFX). Upon incubation with CPFX or Lipo-CPFX, cellular drug accumulation was determined from the cell lysates or efflux was from the extracellular media by fluorescence-HPLC. The accumulation for Lipo-CPFX reached the asymptotic values at ≥ 2 hours, which was a result of uptake and efflux. The uptake appeared to be due to liposomes, mediated via cellular energy-independent mechanism like lipid fusion. In contrast, the efflux appeared to be due to ciprofloxacin, partly cellular energy-dependent, …

Cannabinoid Modulation Of Hiv-1 Tat-Stimulated Adhesion Of Macrophage-Like Cells To The Extracellular Matrix, Johnathan Drevik

Theses and Dissertations

HIV-Associated Neurocognitive Disorders (HANDs) are becoming one of the largest problems in patients infected with HIV-1. The ability of infected cells such as monocytes and microglial cells to act as viral reservoirs causes extreme inflammation in the CNS and has led to several different types of neurocognitive problems. Specifically these HIV-1 infected monocytes are able to secrete inflammatory factors such as the regulatory protein Tat which acts as a chemoattractant for monocytes while also promoting the adhesion of leukocytes to the extracellular matrix (ECM). We have shown that one of the major features of the Tat protein is that it …

Calcium/Calmodulin-Dependent Protein Kinase Ii Beta (Camkiiβ): A Regulator Of Oligodendrocyte Maturation And Myelination, Christopher Waggener

Theses and Dissertations

Oligodendrocytes are cells located in the central nervous system (CNS) that are responsible for the production of the lipid rich membrane, myelin. Myelin and the process of making and wrapping myelin around an axon (also known as myelination) are critical for normal development since they ensure proper signal conduction in the vertebrate CNS. The loss or damage of this myelin, which is typically associated with the demyelinating disease multiple sclerosis (MS), is associated with improper axonal protection along with disrupted nerve signaling which can lead to a variety of different debilitating phenotypic responses. It has been shown that there are …

The Metabolic Transitions Regulated By The Estrogen-Related Receptor (Err) In Drosophila Melanogaster, Yan Li

Theses and Dissertations

In multicellular organism, bioenergetic metabolism is strictly regulated toward efficient generation of ATP. However, in certain situations, such as in limiting oxygen or in the rapidly proliferating system like growing juvenile or cancer cells, organisms apply the metabolic strategy that favors the production of biomass (e.g., nucleotides, amino acids, and lipids) over efficiency of ATP generation. The conserved estrogen-related receptors (ERRs) are master regulators in controlling metabolic homeostasis, and good candidates for mediating the metabolic transition induced by hypoxia and development. First, we investigate how dERR influences hypoxic adaptation in Drosophila melanogaster. We find that dERR is required for a …

Association Of Family Structure And Glycemic Control In Adolescents With Type 1 Diabetes: Risk And Protective Factors, Laura Jean Caccavale

Theses and Dissertations

Youth with type 1 diabetes (T1D) from single-parent families are more likely to be in poorer glycemic control (HbA1c). Demographic trends indicate more households are composed of unmarried adults and fewer youths. Family density, or a youth: adult ratio, may be a more salient factor than single-parent status in the association with glycemic control. Data from 257 adolescents aged 11-14 years (M = 12.84) at two different sites were collected as part of a randomized control trial of a treatment intervention designed to increase parent involvement and prevent deterioration of adolescent diabetes disease care. Single-parent status was determined by parental …

In Vitro Lung Epithelial Cell Transport And Anti-Inflammatory Activity For Liposomal Ciprofloxacin, Ruba Darweesh

Theses and Dissertations

Liposomal ciprofloxacin (Lipo-CPFX) is being developed for inhalation, with a goal of sustaining the therapeutic activity, compared to unformulated ciprofloxacin (CPFX). However, the kinetics and mechanism of its sustained local lung retention and pharmacological activity are yet to be fully characterized. This project hypothesized that Lipo-CPFX enables slower and sustained lung epithelial transport and uptake, compared to CPFX, thereby producing prolonged local pharmacological actions. The human bronchial epithelial Calu-3 cells were used as monolayers to characterize the kinetics and mechanism of transport and/or uptake, and to assess the effects of such slow kinetics for Lipo-CPFX on its inhibition against lipopolysaccharide …

Sphingosine-1-Phosphate And Fingolimod (Fty720) Regulate Icl,Swell In Hl-1 Cardiac Myocytes Via Intracellular Binding And Mitochondrial Ros Production, Pooja Desai

Theses and Dissertations

Swelling-activated Cl− current (ICl,swell) is an outwardly-rectifying current that plays an important role in cardiac electrical activity, cellular volume regulation, apoptosis, and acts as a potential effector of mechanoelectrical feedback. Persistent activation of ICl,swell has been observed in models of cardiovascular disease. We previously suggested sphingosine-1-phosphate (S1P) activates volume-sensitive Cl- current (ICl,swell) by ROS-dependent signaling. S1P and its analog, FTY720 (fingolimod), primarily act via G-protein coupled receptors (S1PR; S1PR1-3 in heart), but several intracellular S1P ligands are known. We investigated how these agents regulate ICl,swell. ICl,swell was elicited by bath S1P (500 nM), FTY720 (S1PR1,3 agonist; 10 μM), and SEW2871 …

Air Circulation Under Reduced Atmospheric Pressures, Lendell E. Hillhouse

Theses and Dissertations

The control of heat exchange is vital for plant life in off-world, low pressure,

greenhouses. The ability to control this process was limited by methodology and

technology. Mathematical models, based on classical mechanics are created to enhance

our control capabilities. Data is collected using various sensors placed inside the Low

Pressure Test Bed (LPTB) Chamber at Kennedy Space Center. Data from those sensors

became non-linear at various pressures below 25 kPa. We introduced mathematical

calibration corrections and found that sensor data linearity could be extended to a greater

range of pressures. These calibration corrections allow for sensor calibration corrections

in …

Women's Use Of Intimate Partner Violence: An Examination Of Attachment, Coping, And Gender Ideology, Theresa Elizabeth Magelky

Theses and Dissertations

Until recently, much of the research on intimate partner violence has focused primarily on male aggressors. However, research has increasingly indicated that women use violence against male intimate partners at higher rates than previously suspected. Significant controversy exists with regard to the context, motivation, and types of violence used by women. The current study explored the degree to which female aggressors' attachment styles are associated with and predictive of their use of intimate partner violence against men. In addition, coping processes and feminine ideology were studied, particularly in combination with attachment style, to determine their relationship with use of partner …

An Investigation Of Gender's Role In Three- And Four-Year-Old Children's Musical Play, Kari Jo O'Keefe

Theses and Dissertations

Young children engage in many activities, with play being the most prominent. Musical play occurs when children freely investigate sound through vocal, instrumental, or other means. Identifying the extent of musical play - and the role that gender plays in musical play - may provide important information about children's natural tendencies.

The purpose of this study was to investigate gender's role in three- and four-year-old children's musical play, and to investigate further the general nature of musical play. A grounded theory methodology was used, and data was gathered from field and individual child observations, and parent and preschool teacher surveys. …

Examining The Continuity Of The Long-Lived (Triassic-Recent) Freshwater Mussel Genus Diplodon (Family Hyriidae), Matt Burton-Kelly

Theses and Dissertations

This paper addresses the question of how strong the record of contiguity is for the 250 million-year-old Diplodon lineage by examining the geographic and temporal distribution of fossil specimens identified as Diplodon.

Diplodon (Mollusca, Bivalvia, Unionoida, Hyriidae) has a fossil record extending back to the Middle Triassic (Anisian Stage). The known distribution of fossil specimens identified as this genus occurs on four continents (North America, South America, Australasia, and Antarctica). The place of origin and pathways of range expansion through time are far from well explained. Both fossil and extant freshwater mussel taxa are subject to evolutionary and phenotypic …

Sexual Fluidity And Women's Bisexual Identity Development, Julijana Draganic

Theses and Dissertations

Experiences of bisexual and sexual fluid women are poorly explored and understood in the current literature. While there is a need and interest in the exploration of bisexual experiences in the current literature, these experiences are usually lumped together with the experiences of lesbian women, who are vastly overrepresented in the participant samples. Data was collected from LGBT centers, women's centers, bisexual interest listservs, and sexual minority internet groups. This study examined: the relationship between a measure of fluidity in attractions, the Sexual Fluidity Grid (SFG) and a measure of sexual orientation, the Klein Sexual Orientation Grid; the relationship between …

Climate Change And Ragweed Allergy Risk Assessment: A Study Of The Grand Forks Metropolitan Statistical Area, Shumila Rani Ahmad

Theses and Dissertations

Nowadays the association between anthropogenic climate change and public health is in the early stages of universal recognition. Yet one fundamental aspect that remains largely unappreciated is the impact of climate change on ragweed (Ambrosia species) biology and the ensuing pathophysiology of human systems. Ragweed pollen is one of the primary causes of seasonal pollen allergy in the world. Allergic reactions to ragweed pollen can range from mild hay fever to life-threatening asthma attacks. The aim of this thesis was to conduct a ragweed allergy risk assessment for the Grand Forks ND-MN Metropolitan Statistical Area (MSA).

The study area is …

Vascular Endothelial Growth Factor In Cortical Development, Jacob Thomas Cain

Theses and Dissertations

One of the characteristics unique to mammalian evolution is the development of the neocortex. The neocortex is a highly ordered six-layered structure, the development of which is tightly regulated. The formation of the cortex is concomitantly dependent upon an outward expansion of the neuroepithelium, a tissue from which all of the neuronal subtypes and glia are born and differentiated, and the investment of blood vessels from the outer pial surface. Orchestration of neurogenesis and blood vessel investment, or angiogenesis, in the cortex is critical for development; however, these processes are often studied independent of one another. While independent investigation of …

Determination Of Optimum Polymer Dose For Grand Forks Wastewater Treatment Plant Lagoon Sludge Dewatering, Dipesh Das

Theses and Dissertations

The Grand Forks Waste Water Treatment Plant (GFWWTP) is currently sending its waste activated sludge (WAS) from the activated sludge treatment process to an existing on-site wastewater treatment lagoon which has been in operation since 2003. The plant produces approximately 65,000 gallons of WAS per day. Because of this high level of loading, the existing lagoon system is no longer considered as a treatment option for the produced sludge. The Plant Authority is trying to find a sustainable solution for sludge disposal and for this reason the GFWWTP is interested in introducing screw press system for the dewatering process. As …

Epigenetic Patterns: Process-Driven Or Sequence-Driven?, Amanda Gefroh

Theses and Dissertations

Some of the most rapidly-evolving fields of study in the medical and research communities presently are those which investigate our genetic code, the elements and factors that control the expression of our genetic code, as well as fields of study which analyze theories about an organisms' physiology and phenotype from the information that both of the previous topics concomitantly provide. The work of the Milavetz lab, among many others, is striving to fill the gaps in knowledge about epigenetic regulation of biological processes - here by using the well-suited model virus, Simian Virus 40. It has for some time now …

A Mass Spectrometry-Based Method For Quantification Of Human Metallothionein Isoforms And Usefulness In Biomarker Detection Of Cancers And Metal Toxicity, Aaron Andrew Mehus

Theses and Dissertations

Current methods for detecting metallothionein (MT) protein expression lack the specificity to distinguish between all twelve human isoforms. Each, however, can be distinguished by the masses of their acetylated, cysteine-rich, hydrophilic N-terminal tryptic peptides. These properties were exploited to develop a mass spectrometry-based method for their simultaneous quantification.

Human kidney HK-2 epithelial cells expressing recombinant MT-3 were grown in the presence or absence of cadmium. Cytosolic proteins were alkylated with 14N- or 15N-iodoacetamide and digested with trypsin. The N-terminal MT peptides were enriched by two-dimensional liquid chromatography and analyzed by MALDI-TOF/TOF mass spectrometry. Relative expression was measured by combining 14N-labeled …

The Ecology Of Host-Seeking Mosquitoes Within The Red River Valley Of Central North Dakota, Joseph Orlo Mehus

Theses and Dissertations

Host-seeking mosquitoes are taxing for people and wildlife alike in the Red River Valley (RRV). During the summer massive numbers of mosquitoes swarm the RRV, yet little is known about the ecology and biology of the mosquito species that inhabit this area. This research will help to fill some of those knowledge gaps by studying the ecology of host seeking mosquitoes in the RRV.

Host-seeking mosquitoes were collected using CO₂-baited MMX^TM traps. Trapping was conducted in two very different rural settings within the RRV. One site, a 40-acre hardwood forest with closed canopy, the other a farmstead …

Epileptic Seizure Detection And Prediction From Electroencephalogram Using Neuro-Fuzzy Algorithms, Ahmed Fazle Rabbi

Theses and Dissertations

This dissertation presents innovative approaches based on fuzzy logic in epileptic seizure detection and prediction from Electroencephalogram (EEG). The fuzzy rule-based algorithms were developed with the aim to improve quality of life of epilepsy patients by utilizing intelligent methods. An adaptive fuzzy logic system was developed to detect seizure onset in a patient specific way. Fuzzy if-then rules were developed to mimic the human reasoning and taking advantage of the combination in spatial-temporal domain. Fuzzy c-means clustering technique was utilized for optimizing the membership functions for varying patterns in the feature domain. In addition, application of the adaptive neuro-fuzzy inference …

Spirituality And Systemic Lupus Erythematosus, Kara Ann Richardson-Cline

Theses and Dissertations

Problem: The positive relationship between spirituality and physical, psychological, and social well-being has been well established. However, beyond quality of life measures, this construct has yet to be more deeply studied in a sample of individuals diagnosed with Systemic Lupus Erythematosus (SLE). Furthermore, relational spirituality has yet to be studied in a chronically ill population. Meeting these gaps in the literature, this study compared the explicit domains and latent constructs measured by a relational spirituality instrument and examined their associations with a measure of biopsychosocial-spiritual well-being and quality of life measure.

Results: Utilizing nonrandom online surveys, this study included 305 …

Towards More Efficient Enhanced Sampling Methods To Study Phase Transitions, Kenneth Ndumbe Ngale

Theses and Dissertations

The most familiar phase transitions observed in nature are associated with a change in the state of matter (solid, liquid, and gas). In some rare cases this may involve the plasma phase. Such transitions are often referred to as first order phase transitions and often occur commonly such as during the melting of snow or freezing of lakes and rivers during winter. This project focuses on the most ubiquitous phase changes such as, liquid-solid and vapor-liquid as well as the less prevalent vapor-solid transitions. These types of phase transitions are also known as classical phase transitions. They usually involve symmetry …

Wendell Berry's Philosophy Of Education: Lessons From Port William, Jane Margaret Schreck

Theses and Dissertations

In the midst of a proclaimed crisis in higher education, in the clamor and clamber to leverage technology for such innovations as mass open online courses and differentiated learning modules, in the speculative frenzy of preparing students for the careers of a fantasy future, and in the swirl of angst about funding accountability and economic relevance, Wendell Berry's philosophy of education declares that the essential element missing from most current discussions and considerations of education is love. As explained in his essays and revealed in his fiction and poetry, Berry's philosophy centers on love as the best animator of learning: …

Acetate Increases Brain Purine Metabolism And Glial Cell Culture Fatty Acid Content: Studies Investigating Glyceryl Triacetate's Therapeutic Mechanism Of Action, Dhaval Paresh Bhatt

Theses and Dissertations

Acetate supplementation is anti-inflammatory and neuroprotective in rat models of neuroinflammation and Lyme neuroborreliosis. Oral administration of glyceryl triacetate (GTA) is used to induce acetate supplementation in rodents, which increases plasma acetate and brain acetyl-CoA levels. Acetyl-CoA is a ubiquitous substrate for several biochemical pathways including carbohydrate, protein, and lipid metabolism. Acetate increases the in vivo and in vitro acetylation-state of histones and is associated with altered cytokine balance. However, the effect that acetate supplementation has on brain purine and lipid content remains unknown. Since labeled-acetate is incorporated into the purine and lipid pools, we proposed that acetate supplementation by …

Characterization Of Phosphorus, Nitrogen, And Sulfur Concentrations In A Paired Disturbed And Natural Wetland In Northwest Minnesota, Nicolas Buer

Theses and Dissertations

A degraded wetland with a history of human-induced hydrologic alterations lies within northwestern Minnesota's Skull Lake Wildlife Management Area (WMA). Although conservation practices have been enacted by the Minnesota Department of Natural Resources (MNDNR), there remains interest in the wetland's potential for ecological restoration. Restoration should not be undertaken without an understanding of underlying factors leading to degradation. A paired study between the disturbed wetland at Skull Lake WMA and the relatively natural wetland in the nearby Caribou WMA was designed to help understand near surface pore water geochemistry in an effort to determine causes of degradation and the potential …

Effects Of Parental Support On Physical Activity Levels In Children, Julie Ann Caspers

Theses and Dissertations

Introduction: The literature is mixed regarding behavioral methods that may effectively motivate children to increase physical activity levels. Because some research has shown parental influence can affect children's behavior, it is hypothesized that trained parental support may increase children's physical activity.

Purpose: The purpose of this study was to examine the effects of parental support on children's physical activity. Pender's Health Promotion Model (HPM) served as a guide. The theoretical underpinnings of this model are based on the assumption that individuals are influenced by interpersonal factors such as parental support.

Design and Sample: For this pre-test/post-test interventional study, 30 children …

Osteon-Mimetic Nanocomposite Materials For Bone Regeneration, Ozan Karaman

Theses and Dissertations

The limitations of large bone defect regeneration has brought the focus of Bone Tissue Engineering research on fabricating three-dimensional bone-mimetic grafts that could enhance osteogenesis and vascularization. Osteons composed of apatite-deposited aligned collagen nanofiber layers are the fundamental building blocks of cortical bone. In natural cortical bone, mineralization is mediated by extracellular matrix (ECM) proteins with amino acid sequences rich in glutamic acid. This dissertation is focused on the establishment of a novel biomimetic approach to engineer scaffolds for healing large bone defects.

In the first part, we investigated the effect of calcium phosphate (CaP) deposition on aligned surface-modified nanofibers …

Effect Of Physiological Oxygen Levels On Osteogenic Differentiation Of Adipose-Derived Stem Cells, Suchit Sahai

Theses and Dissertations

Regenerative medicine presents exciting strategies for healing critical-size bone defects through the implantation of cells and biocompatible scaffolds. Most in vitro studies are performed in atmospheric oxygen conditions (~20%), which do not accurately mimic the CSD microenvironment. Due to damage to the vasculature at CSDs, oxygen levels fall into the hypoxic range (<5%), which can impact viability, proliferation and differentiation of the cells employed for bone regeneration. Understanding the cellular responses to hypoxia has grown primarily from study of individual molecular factors. The master regulator of adaptive responses to low oxygen availability is the nuclear factor, hypoxia-inducible factor-1. HIF-1 is a heterodimeric protein, stable below 6% O2 condition in the nucleus and has been shown to play a role in angiogenic-osteogenic coupling. We have developed a responsive, fluorescent, hypoxia detection system and determined whether HIF activity can be tracked in both 2-D and 3-D cultures. Adipose-derived stem cells (ASCs) were selected due to their broad utilization in tissue engineering strategies and characterized the influence of HIF signaling on its phenotype. The work done identified that of hypoxia impaired osteogenic differentiation of ASCs in both 2-D and 3-D cultures and HIF-1 did not mediate this effect. Ischemic preconditioning (IPC) strategy and varied protocols used represents a clinically feasible manipulation of cell preparation to help the survival of implanted ASCs and accelerate osteogenic differentiation at physiological oxygen levels.

Characterizing Hypoxia And Its Behavioral Effects In 3-Dimensional Cell Aggregates, Matthew Lorincz Skiles

Theses and Dissertations

Cell transplantation can be considered a regenerative therapy, an intervention which attempts to replace or restore the function of compromised tissue by harnessing innate properties of cells that cannot be replicated artificially. For such therapies to succeed, it will be necessary to understand and closely match the physiological conditions that govern cell behaviors in vivo. One important factor is low oxygen tension, termed hypoxia, which is often overlooked in vitro. Because oxygen insufficiency can lead to cell death, hypoxia has traditionally been viewed as a negative condition. However, hypoxia can also serve as a potent regulator of crucial cell behaviors …

Evaluation Of Feeding Varying Levels Of Digestible Lysine On Broiler Breeder Male Reproductive Characteristics And Body Weight Changes, Chinwendu Nkechi Obi

Theses and Dissertations

A preliminary test was conducted evaluating the effect of digestible lysine (dLys) on broiler breeder (BB) male semen quality from forty-one to forty-nine wk of age. Five dietary treatments: corn-soybean meal diet with 1,000 mg dLys/rooser/day (Soy1,000), distillers dried grains with solubles diet with 1,000 (DDGS1,000), 850 (DDGS850), 700 (DDGS700), and 550 (DDGS550) mg dLys/rooster/day. Semen quality was similar except percentage dead sperm which was higher in DDGS550. A second trail was conducted using the same dietary treatments as the preliminary test during twenty to thirty-nine wk of age. Semen quality was similar except percentage dead sperm which was higher …