Digital Commons Network^™

Selection And Clustering For Disease Associated Genetic Variants, Yubo Zou

Theses and Dissertations

Epigenetics is the study of chemical reactions, which are orchestrated for the development and maintenance of an organism. Genetic or epigenetic variants (GEVs) encompass different types of genetic measures, such as Deoxyribonucleic acid (DNA) methylation at different CpG sites, expression level of genes, or single nucleotide polymorphisms (SNPs). With the development of technology, huge amount of genetic and epigenetic information is produced. However, the rich information potentially brings in challenges in data analyses. Thus it is necessary to reduce the dimension of data to improve efficiency. In my dissertation, I will focus on two directions of dimension reduction: variable selection …

Computational Analysis And Prediction Of Genome-Wide Protein Targeting Signals And Localization, Jhih-Rong Lin

Theses and Dissertations

Computational prediction of protein subcellular localization can greatly help to elucidate its functions. Despite the existence of dozens of protein localization prediction algorithms, the prediction accuracy and coverage are still low. Several ensemble algorithms have been proposed to improve the prediction performance, which usually include as many as 10 or more individual localization algorithms. However, their performance is still limited by the running complexity and redundancy among individual prediction algorithms. In the first part of the dissertation, we propose a novel method for rational design of minimalist ensemble algorithms for practical genome-wide protein subcellular localization prediction. The algorithm is based …

A Sustainable Bio-Solids Management For The Grand Forks Wastewater Treatment Plant, Hasibul Hasan

Theses and Dissertations

The Grand Forks Waste Water Treatment Plant (GFWWTP) is currently sending its waste activated sludge (WAS) from the activated sludge treatment process to an existing on-site wastewater treatment lagoon which has been in operation since 2003. The plant produces approximately 65,000 gallons of WAS per day. Because of this high level of loading, the existing lagoon system is likely to get replaced by a more sustainable treatment option. Several methods were considered and studied thoroughly for this research, and - on site land application shows some potential. After surveying the Municipal Waste Water Treatment Facilities of the five neighboring states …

Putting The Cart Before The Horse: Understanding The Family Assessment Process In Early Intervention, Kristen Michelle Votava

Theses and Dissertations

The purpose of this study was to gain an understanding of six state-wide policies and procedures used in the family assessment process within early intervention services. This study looked at the administrative understanding of the family assessment federal regulations, state policies and procedures, and local implementation from the perspective of the Part C coordinator in his or her state.

This qualitative study utilized methodologies associated with a grounded theory approach through in-depth interviews. The participants in this study included six state Part C coordinators. Based on the findings of this study, two broad conclusions are offered:

1. There was a …

Grassland Songbird Response To Landscape Composition And Vegetation In Northeastern And East-Central North Dakota, Dustin James Vanthuyne

Theses and Dissertations

Grassland songbirds are declining faster and more consistently than other avifauna of North America. This has resulted in many species being placed on state and federal lists as species of concern. These declines can be linked to former and current land use practices which have resulted in an extensive loss of habitat. Natural resource agencies are trying to offset these habitat losses by reconstructing grasslands formerly cropped areas and protecting remaining grassland tracts. Few studies have addressed how the location in the landscape and vegetation composition of these reconstructions affects grassland birds. This thesis describes data collected from 32 locations …

How Culture Shapes Personality: A Preliminary Study, Hing Man Amy Wong

Theses and Dissertations

The purpose of this study was to explore the relevance of a collectivistic personality attribute indigenous to the Chinese culture to non-Asian collectivistic cultures. To measure this attribute, the CPAI-2 Interpersonal Relatedness factor scales were administered to current college students who self-identified as Mexican American, Chinese American, or Caucasian. Only less acculturated Mexican American and Chinese American participants, as measured by the ARMSA-II or SL-ASIA, respectively, were included in the analysis. Although the Mexican American and Chinese American participants did not significantly differ from one another, these two groups did significantly differ from the Caucasian participants on two scales, Traditionalism-Modernity …

An Action Research Study Of Mexican Teachers' And Administrators' Experiences In Developing And Implementing A Service Learning Program, Kym Acuña

Theses and Dissertations

The purpose of this study was to examine the High Tec Middle School teachers' and administrators' knowledge of the service learning model upon which the school's service learning program has been based, their views regarding their role in student character formation through service learning, and the teachers' feelings of ownership regarding the service learning program. 1 overarching research question and 3 sub-questions were explored. The overarching question was: How do teachers and administrators at High Tec Middle School experience service learning? 3 sub-questions that aided in answer the overarching research question were: What is the High Tec Middle School teachers' …

Morally Injurious Symptomatology: A Qualitative Examination Of The Nvvrs, Douglas Stewart Kraus

Theses and Dissertations

As with all psychological constructs in their infancy, it is important to operationalize a definition as part of the construct validation process. As a phenomenon that continues to gain recognition amongst the psychological community, Moral Injury (MI) is no different. Although Litz et al. (2009) introduced and defined MI as the "psychological, biological, spiritual, behavioral, and social impact of perpetrating, failing to prevent, or bearing witness to acts that transgress deeply held more beliefs and expectations," Drescher et al. (2011) found that a similar working definition was "inadequate" and lacked clarity according to trauma experts' opinion. The following exploratory, qualitative …

Motivation Towards Learning Perceived In Socratic Seminar Versus Traditional Lecture, Benjamin N. Roberson

Theses and Dissertations

As discussed in past literature, high school students often lack motivation towards learning (Crow, 2007; Lumsden, 1995). This lack of motivation interferes with student learning (Lumsden,1995; Vansteenkiste, Simons, Lens, Soenens, & Matos, 2005). At the middle school and collegiate level, Socratic Seminar is seen to provide motivation towards learning in students (Copeland, 2005; Mee, 2000; Strong, 1996); however, there is a need for research on student motivation as a result of Socratic Seminar at the high school level. The purpose of this study is to identify the extent to which, if any, differences exist in student motivation towards learning among …

Comparative Analysis Of Corporate Culture In A Multinational Organization, Don G. Gilman

Theses and Dissertations

This study built upon the Project GLOBE (House, Hanges, Javidan, Dorfman, & Gupta, 2004) analysis by using a Web-based version of the GLOBE Questionnaire in order to examine the extent to which the cultural values and practices of middle managers in a multinational organization vary depending on (a) their cultural background and the region in which they work, (b) whether they were a member of a recently acquired company, and (c) the number of years employed by the multinational organization. Multinational organizations face the unique challenge of operating in societies that have different sets of cultural norms, expectations, beliefs, and …

New Site Administrators' Perceptions Of Their Role In School Community Partnerships, Denise Calvert-Bertrand

Theses and Dissertations

This study's purpose was to investigate new site administrators' perceptions of the term community involvement, of their role to engage the local community members as partners in their school, their preparation and support to work with their communities, and their challenges on-the-job with community engagement. This study also examined new site administrators' perceptions and needs to better understand what tools are necessary to help them create thriving community partnerships. Thirty new site administrators across 4 counties of Southern California participated in a semi-structured 45-minute interview. All were employed less than 4 years and represented the gender, age and ethnic diversity …

Signs And Symptoms Of Moral Injury In Female Vietnam Veterans: A Qualitative Examination Of The Nvvrs, Alison H. Conway

Theses and Dissertations

It is well-known that war veterans experience long-lasting physical and psychological injuries following their deployment, and recent studies have proposed the construct of moral injury (MI) to explain the moral and spiritual effects of war on individuals. Litz et al. (2009) defined MI as "perpetrating, failing to prevent, or bearing witness to acts that transgress deeply held moral beliefs and expectations" (p. 6), and Drescher et al. (2011) interviewed trauma experts to identify types of events that could create MI and the lasting sequelae of these events. Vargas et al. (2013) provided validation of MI by examining male Vietnam veterans' …

The Effects Of Public School Music Education Programs On Leadership In The Workplace, Lawrence Michael Jacobson

Theses and Dissertations

Throughout history, music has consistently played a large role in helping to define the social, historical, and cultural awareness in human society. For children and young adults who have actively studied music in public school music education programs, music has enhanced their self-discipline, self-esteem, and creativity. Over the past 20 years, there have been several examples of how the business world has been turning to the music world for leadership and inspiration. There are, however, few studies that compare the effects of public school music education programs on leadership in the workplace. Researchers have primarily focused their studies on the …

The Politics Of Homosexuality In The Twentieth Century Black Freedom Struggle, Glenda Elizabeth Sherouse

Theses and Dissertations

This project examines the changing status and role of queer African Americans during the twentieth century in the context of increasing black politicization. Moreover, it traces the relationship between queer African Americans and their communities until the emergence of queer black identity politics in the 1970s. Specifically, this dissertation seeks to more fully illuminate both the experiences of queer African Americans and how their communities and leaders discussed and dealt with homosexuality in the broader context of black political mobilization from the Jim Crow era and the Harlem Renaissance, through the Black Power and early Gay Liberation movements, roughly the …

School Psychologist Leadership In Rti Implementation: Administrators' View Of School Psychologists As A Resource In Rti, Allison Rice

Theses and Dissertations

The purpose of this study is to examine the leadership role of school psychologists in Response to Intervention (RTI) implementation, as well as school psychologists' usefulness to principals in successful implementation of RTI. The researcher asserts, that through the fulfillment of certain roles, school leaders recognize the benefit of involving school psychologists in RTI implementation. The study asks the following questions: 1) To what extent are the specific leadership characteristics associated with school psychologists' roles related to the level of RTI implementation being exemplified? 2) To what extent are the specified school leadership characteristics present in school psychologists in Dorchester …

Elucidation Of Mechanisms Generating 5-Hydroxymethylcytosine (5hmc) In Mammalian Mitochondria, Prashant Thakkar

Theses and Dissertations

DNA methylation plays a pivotal role in governing cellular processes including genomic imprinting, gene expression, and development. Recently, the Tet family of methylcytosine dioxygenases(Tet1, Tet2 and Tet3) was found to catalyze the oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), an intermediate in the pathway of DNA demethylation. Tet enzymes catalyze this hydroxylation in a 2-oxoglutarate and Fe2+ dependent manner. We have recently reported significant levels of 5mC and 5hmC modification in immunoprecipitates of mammalian mitochondrial DNA(mtDNA). We provide the first evidence that a DNA Methyltransferase-1 isoform (mtDNMT1) translocates to the mitochondria using an N-terminal mitochondrial targeting sequence. mtDNMT1 expression is …

Hormone Epimers Regulate Er Stress And Core Regulatory Genes: Network Analysis With Applications To Glioma And Chronic Pressure Ulcers, Thomas L. Shaak

Theses and Dissertations

DHEA has been determined to have medically significant activity and is the parent compound to the more active metabolites; 17α-AED, 17β-AED and 17β-AET, which exhibit strong biological activity that has been attributed to androgenic, estrogenic or anti-glucocorticoid activity in vivo and in vitro. This study compared DHEA, 17α-AED, 17β-AED and 17β-AET for their ability to activate the human AR, ER and GR receptors and determine the relative androgenicity, estrogenicity and glucocorticoid activity. The results show that, at the receptor level, these androstene hormones are weak AR and even weaker ER activators. Direct androstene hormone activation of the human AR, ERα, …

Assessment Of The Feasibility Of Co-Administration Of Phenolic Dietary Compounds With Phenylephrine To Increase Its Bioavailability, Zhenxian Zhang

Theses and Dissertations

R-(-)-Phenylephrine (PE) is the most commonly used nonprescription oral nasal decongestant in the United States. It is a selective α1-adrenergic receptor agonist and has many years of safe usage. However, the efficacy of PE is controversial, due to its extensive pre-systemic metabolism, which leads to low and variable oral bioavailability (38 ± 9%, mean ± SD). Sulfation plays a very important role in pre-systemic metabolism of PE. The sulfation of PE occurs at its phenolic group, which is the preferred structural feature of many sulfotransferase (SULT) substrates. Compounds with phenolic groups have similar structures to PE, which may share the …

In-Vitro Pk/Pd Profiling And Modeling Of The Anti-Sickling Agents, 5-Hydroxymethyl Furfural (5-Hmf) And Novel Synthetic Allosteric Effectors Of Hemoglobin (Aeh) In Human Whole Blood, Apurvasena Parikh

Theses and Dissertations

Introduction. 5-HMF and novel INN-compounds are left-shifting AEH, shown to have anti-sickling action by forming transiently covalent Schiff-base adducts with hemoglobin (Hb), thereby increasing the Hb O2-affinity. They are hypothesized to be substrates for aldehyde dehydrogenase (ALDH) in the liver and red blood cells (RBC). Methods. Biopharmaceutical assessments were made for AEH, using calculated physicochemical properties. Their in-vitro hepatic metabolism (mediated by ALDH) was characterized using hepatic cytosol, and in-vitro-in-vivo extrapolations (IVIVE) were made. Inter-species differences in hepatic cytosolic ALDH activity were investigated using acetaldehyde as a model substrate in different mammalian species. Time- and concentration-dependent in-vitro disposition of 5-HMF …

Thc-Mediated Induction Of Δfosb And Its Modulation Of Cb1r Signaling And Adaptation, Lazenka Matthew

Theses and Dissertations

The main psychoactive and therapeutic effects of Δ9-tetrahydrocannabinol (THC) are mediated through cannabinoid type 1 receptors (CB1Rs). The therapeutic uses of THC are mitigated by the development of tolerance to these therapeutic effects, whereas tolerance does not readily develop to some of the side-effects of THC, like motor impairment and reward. The development of tolerance occurs through adaptations at CB1Rs, which include desensitization (G-protein uncoupling) and downregulation (receptor degradation). Brain region-dependent differences in THC-mediated adaptations are proposed to explain the differences in tolerance to various THC-mediated effects. These studies focused on whether ΔFosB, a stable transcription factor, could regulate CB1R …

Graphene-Supported Metal Nanoparticles For Applications In Heterogeneous Catalysis, Hany Elazab

Theses and Dissertations

Due to its unique properties and high surface area, Graphene has become a good candidate as an effective solid support for metal catalysts. The Nobel Prize in Physics for 2010 was awarded to Andre Geim and Konstantin Novoselov "for groundbreaking experiments regarding the two-dimensional material graphene". Microwave-assisted synthesis of various metallic nanostructured materials was investigated for CO oxidation applications. These metallic nanostructured materials were used to convert CO to CO2 as an effective approach for carbon monoxide elimination due to its harmful effect on health and environment. In particular, this dissertation is focusing on palladium as a transition metal that …

Communication Channels For Cancer Survivorship: Addressing Information Dissemination, Clinical Trial Recruitment And Social Networking, Carly Elizabeth Steen

Theses and Dissertations

More people are surviving cancer than ever before due to early detection and advances in treatment (Kazanjian, Smillie, Howard, Ward and Doll, 2009). With a growing group of cancer survivors, a group expected to grow to 18 million by 2020, additional informational resources must be put in place (Cancer Survivorship Training, 2013). This study seeks to better understand 1) where cancer patients and survivors are finding cancer resources, 2) what computer-mediated communication channels they use and prefer and 3) who they trust the most to educate and recruit them for clinical trials. Twenty in-depth interviews were conducted and analyzed with …

Deconstructing Books, Reconstructing Women, Summers Esther Morris

Theses and Dissertations

The goal of my thesis project is to examine the notion of choice encountered by females with respect to gender roles. I intend to underscore the turmoil which often comes from choices that leave many classic feminine roles left unattended by either sex. I have created three altered books that address these struggles, as I have personally experienced these, regarding marriage, motherhood, and domesticity. Each book analyzes the conflicting nature of choice and, according to my own decisions as a modern feminist, concludes accordingly. This in-depth look at the struggle for women to not only choose gender roles to adopt, …

Nutrient Limitation Of Bioluminescent Dinoflagellates In Mangrove Lagoon, Salt River Bay, St. Croix, Usvi, Michelle Zimberlin

Theses and Dissertations

Bioluminescent bays resulting from dense concentrations of bioluminescent dinoflagellates are rare, with only 14 documented systems worldwide. One of these bays, Mangrove Lagoon, is located in Salt River Bay National Park in St. Croix, USVI. Very little is known about the environmental factors responsible for maintaining the high dinoflagellate densities in this lagoon. In order to assess the dynamics of the dinoflagellates, in situ nutrient addition bioassays were conducted to determine which nutrients regulate the phytoplankton community, which includes the bioluminescent dinoflagellate Pyrodinium bahamense. Bioassays were conducted in two different seasons, (January 2013 and May 2013) to examine seasonal responses …

Uncovering The Structures In Ecological Networks: Multiple Techniques For Multiple Purposes, Peng Gao

Theses and Dissertations

Ecosystem structure and function are the product of biological and ecological elements and their connections and interactions. Understanding structure and process in ecosystems is critical to ecological studies. Ecological networks, based on simple concepts in which biological and ecological elements are depicted as nodes with relationships between them described as links, have been recognized as a valuable means of clarifying the relationship between structures and process in ecosystems. Ecological network analysis has benefited from the advancement of techniques in social science, computer science, and mathematics, but attention must be paid to whether the designs of these techniques follow ecological principles …

Electrokinetic Mixing And Separation In Microfluidic Systems, Fang Yang

Theses and Dissertations

Electrokinetics involves the study of liquid or particle motion under the action of an electric field; it includes electroosmosis, electrophoresis, dielectrophoresis, and electrowetting, etc. The applications of electrokinetics in the development of microfluidic devices have been widely attractive in the past decade. Electrokinetic devices generally require no external mechanical moving parts and can be made portable by replacing the power supply by small battery. Therefore, electrokinetic based microfluidic systems can serve as a viable tool in creating a lab-on-a-chip (LOC) for use in biological and chemical assays. Here we present our works of electrokenitic based mixing and separation in microfluidics …

A Preliminary Study Exploring Racial Differences In Triage, Hospitalization Status, And Discharge Medication In An Emergency Department In Graniteville, Sc, Clare Pollock

Theses and Dissertations

Purpose

This study seeks to identify any racial differences present in assigned triage scores, hospitalization status and discharge medications in a rural hospital likely experiencing a surge after a mass casualty incident involving chlorine gas as a result of a train derailment. Differences were examined between Non-Hispanic White and African American adults who were moderately ill and who presented to the emergency department of the closest hospital to the accident site within 24 hours of the incident.

Methods

Non-Hispanic White and African American adults who presented to the emergency department of the closest hospital to the accident site and who …

Modeling Approaches, Physiological Responses, And Climate Change: How Good Is "Good Enough?", Nicole Kish

Theses and Dissertations

Predicting spatial and temporal patterns in the responses of organisms and ecosystems to climate change has emerged as a major focus of macrophysiological research, with much work centered on the impacts of temperature. A potential difficulty lies in the observation that measures of 'habitat' such as air, land and sea surface temperature often differ greatly from the body temperature actually experienced by organisms, as the latter drives reproduction and survival. As a result, it is unclear how often these simple measurements of habitat are 'good enough' for predicting physiological stress in the field, and when more complicated methods are needed. …

I Can Be Silent And Be Saying A Lot: Teachers' Racial Literacy In A Southern Elementary School, Kimberly J. Howard

Theses and Dissertations

In order to better understand how teachers make sense of race in schools today, this ethnographic study explores the following research question: How do teachers in this school make sense of race, and how does the spatiality of the school inform this process? The study was conducted over a 14-month period in a southern elementary school and is presented as a poetic, narrative, and thematic analysis of the connections between the geographic location of this particular school and the teachers' practices, pedagogies, and conversations about race both inside their classrooms and in other school spaces. This study demonstrates how teachers' …

Amplitude Vs. Offset Effects On Gas Hydrates At Woolsey Mound, Gulf Of Mexico, Walter R. Anderson

Theses and Dissertations

Due to the estimated massive quantities of natural methane hydrates, they represent one of the largest sources of future alternative energy on Earth. Methane hydrates have been found in the shallow sub-seafloor of the Northern Gulf of Mexico where the water depth is in excess of ~900 m. Mississippi Canyon Block 118 has been chosen by the Gulf of Mexico Hydrates Research Consortium to be the site of a multi-sensor, multi-discipline sea-floor observatory for gas hydrate research. First evidence for gas hydrates at MC 118 was observed at Woolsey Mound. Subsurface evidence for gas hydrates has subsequently been substantiated by …