Digital Commons Network^™

Enhancing Protein And Enzyme Stability Through Rationally Engineered Site-Specific Immobilization Utilizing Non-Canonical Amino Acids, Jeffrey Chun Wu

Theses and Dissertations

The demand for economical, efficient protein production, reuse, and recovery has never been greater due to their versatility in a large variety of applications ranging from industrial chemical manufacturing to pharmaceutical drug production. The applications for naturally and artificially produced proteins include protein drugs and other pharmaceutical products, as biocatalysts in environmentally friendly chemical manufacturing, as enzymes for food processing purposes, and as an essential component in many biomedical devices. However, protein production suffers from many challenges, which include the cost of production, protein stability especially under harsh conditions, and recoverability and reusability of the proteins. The combination of two …

Effect Of Thermal Treatments On Mechanical Properties And Bioactivity Of Third Generation Of Bioactive Glass. A Step For Commercialization Success Of Third Generation Bioactive Glasses, Rajan Pandya

Theses and Dissertations

Unavailability of donors for transplanting bone to help people with orthopedic imparities gave rise to the metal implants that could be made in factories. This seemed like a good solution as it was available to all the people who needed it. It came with limitations such as restricted movement, infection, wear and tear of the surrounding tissue, and rejection. Biologically active implants were introduced by Dr. Larry Hench in 1960s [12]. The field of regenerative medicine has been changing rapidly since then. Biologically active implants facilitate bone regeneration and do not harm the surrounding tissue in any way. The bioactive …

Metamorphosis Of Limulus Polyphemus Trilobite Larvae: Role Of Chemical And Structural Cues, Competency, And The Cost Of Delayed Metamorphosis, Stephanie Marie Kronstadt

Theses and Dissertations

Many marine and estuarine arthropods exhibit complex life cycles that include planktonic larval stages and benthic juvenile and adult phases. Chemical and structural cues associated with juvenile habitats often induce settlement and metamorphosis, thereby shortening the duration of the larval phase. These cues can trigger metamorphosis only after larvae reach competency, or developmental maturity. The point at which larvae reach this competency period and the ability to retain competency is highly species specific. Once competency is attained, a decrease in the time to metamorphosis (TTM) can decrease dispersal potential but may increase the chance of settling in a suitable habitat. …

Attachment, Vagal Tone, And Co-Regulation During Infancy, Jessica Chloe Hansen

Theses and Dissertations

This study examined the development of attachment as it relates to co-regulation and vagal tone over the second half of the first year of life. Links to infants' attachment and developmental status were also examined. Symmetrical and unilateral co-regulated patterns of interactions at 6 months demonstrated significant linkages with attachment. Developmental status did not show direct linkages with attachment. Direct links between vagal tone and attachment were also not identified. Correlations between co-regulation and vagal tone at the 6 month time point were identified. Findings suggest an important role of co-regulation as it relates to attachment development. Future studies may …

Optimized Photogrammetric Network Design With Flight Path Planner For Uav-Based Terrain Surveillance, Ivan Yair Rojas

Theses and Dissertations

This work demonstrates the use of genetic algorithms as a stochastic optimization technique for developing a camera network design and the flight path for photogrammetricapplications using Small Unmanned Aerial Vehicles. This study develops a Virtual Optimizer for Aerial Routes (VOAR) as a new photogrammetric mapping tool for acquisition of images to be used in 3D reconstruction. 3D point cloud models provide detailed information on infrastructure from places where human access may be difficult. This algorithm allows optimized flight paths to monitor infrastructure using GPS coordinates and optimized camera poses ensuring that the set of images captured is improved for 3D …

Small Mammals Matter? Linking Plant Invasion, Biotic Resistance, And Climate Change In Post-Fire Plant Communities, Rory Charles O'Connor

Theses and Dissertations

The introduction and establishment of exotic species can profoundly alter ecosystems. Two exotic species drastically changing the landscape of deserts in western North America are Bromus tectorum L. and Bromus rubens L. Through the buildup of biomass and slow decomposition rates in deserts these two exotic annual grasses can alter fire regimes that change the plant and animal community dynamics in the ecosystems. To better understand the ecological mechanisms that could restrict or alter the patterns of invasive plant establishment we established a replicated full factorial experiment in the Great Basin and Mojave Desert. The combinations of factors being manipulated …

The Interaction Of Aeolian And Fluvial Processes In Dry Washes On The Colorado Plateau, Usa, Beau Jensen Walker

Theses and Dissertations

In the past decade there has been a call for integrated studies that examine the interaction of fluvial and aeolian processes (Belnap et al., 2011; Bullard and Livingstone, 2002). In this study, we examined the role of land-use, weather, and soil type on the flux of aeolian material into dry washes on the Colorado Plateau in central Utah, USA, and western Colorado, USA. Our goal was to quantify the impact of individual deposition and erosion events by correlating weather and land-use data with a combination of measurement methods including dust collection via dust traps, GPS surveying, and close-range photogrammetry. Our …

Fabrication, Characterization, Optimization And Application Development Of Novel Thin-Layer Chromatography Plates, Supriya Singh Kanyal

Theses and Dissertations

This dissertation describes advances in the microfabrication of thin layer chromatography (TLC) plates. These plates are prepared by the patterning of carbon nanotube (CNT) forests on substrates, followed by their infiltration with an inorganic material. This document is divided into ten sections or chapters. Chapter 1 reviews the basics of conventional TLC technology. This technology has not changed substantially in decades. This chapter also mentions some of the downsides of the conventional approach, which include unwanted interactions of the binder in the plates with the analytes, relatively slow development times, and only moderately high efficiencies. Chapter 2 focuses primarily on …

Role Of Epistasis In Alzheimer's Disease Genetics, Mark T. Ebbert

Theses and Dissertations

Alzheimer's disease is a complex neurodegenerative disease whose basic etiology and genetic structure remains elusive, despite decades of intensive investigation. To date, the significant genetic markers identified have no obvious functional effects, and are unlikely to play a role in Alzheimer's disease etiology, themselves. These markers are likely linked to other genetic variations, rare or common. Regardless of what causal mutations are found, research has demonstrated that no single gene determines Alzheimer's disease development and progression. It is clear that Alzheimer's disease development and progression are based on a set of interactions between genes and environmental variables. This dissertation focuses …

Targeted Sequencing Of Plant Genomes , Mark D. Huynh

Theses and Dissertations

Next-generation sequencing (NGS) has revolutionized the field of genetics by providing a means for fast and relatively affordable sequencing. With the advancement of NGS, whole- genome sequencing (WGS) has become more commonplace. However, sequencing an entire genome is still not cost effective or even beneficial in all cases. In studies that do not require a whole-genome survey, WGS yields lower sequencing depth and sequencing of uninformative loci. Targeted sequencing utilizes the speed and low cost of NGS while providing deeper coverage for desired loci. This thesis applies targeted sequencing to the genomes of two different, non-model plants, Artemisia tridentate (sagebrush) …

Extent Of Cysteine Modification Of Snap-25 In Vitro, Alex Mcgregor Dabell

Theses and Dissertations

Exocytosis, the fusion of a vesicle to a cellular membrane, involves a protein named SNAP-25. This protein, containing two alpha helices connected with a linker region, is localized to the cell membrane via palmitic acids attached to the cysteine residues of its linker region in a process called palmitoylation. Are cysteine residues of the SNAP-25 linker region palmitoylated in an ordered manner and to a particular extent? The answer to this question may give insight into the regulated nature of exocytosis. While it is generally accepted that SNAP-25 must be palmitoylated in order to perform its exocytotic functions, the details …

Linking Land Use And Water Quality Changes In Lake Maumelle Through Benthic Sediment, Dawn Marie Johnson

Theses and Dissertations

Lake Maumelle is a reservoir in Pulaski County, Arkansas that was finished in 1958 for the purpose of supplying potable water to Little Rock and surrounding areas. It is an oligotrophic, monomictic lake with a generously forested watershed. There has been an increase of urbanization in the watershed which may influence the water quality. This research examines the reservoir's sensitivity to land use/land cover change for the lake by analyzing the benthic sediment. By using multidisciplinary methods, cores were examined using mass accumulation rates, grain size analysis, elemental analysis, phase identification, and diatom identification. Those results were then compared to …

Liberal And Conservative Newspaper Reporting Leading Up To The War With Iraq, Shirley E. Pence

Theses and Dissertations

This study seeks to determine whether there was a difference in the news reports by liberal and conservative newspapers up to the 2003 war with Iraq. When no weapons of mass destruction were found in Iraq, the news media was criticized for its failure to challenge the president's "rush to war." Through examination of two liberal and two conservative newspapers' editorials and front page news articles in four periods following the September 11 terrorist attacks, the liberal New York Times and the Washington Post were found to be less supportive than the conservaive Wall Street Journal and the Washington Times …

Using Random Forest In The Field Of Metabolomics, Curtis White

Theses and Dissertations

Metabolomics is the science of comprehensive evaluation of changes in the metabolome with a goal to elucidate underlying biological mechanisms of a living system. There is an opinion in the field of metabolomics, the study of the set of metabolites present within an organism, cell, or tissue, that the future development of the field is contingent upon two factors. One of the factors is the advancement of analytical instrumentation, and the other is developing data mining methodologies for extracting meaningful and interpretable experimental results. There are many different types of data mining methodologies, but the undertaking of selecting a particular …

Preventing Sleep Deprivation In Shift Nurses: What Is The Best Evidence?, Rhonda D. Wilkins

Theses and Dissertations

Insufficient sleep can lead to a variety of cognitive problems such as impaired learning ability, slow thought processes, slower reaction time, poor mood, risky decision-making, inattention, diminished communication skills, and the danger of falling asleep during critical times. Sleep deprived shift nurses are a risk for making erTors and developing health problems. The purpose of this independent study is to detennine the best evidence to prevent sleep deprivation in shift nurses.

A comprehensive review of the literature was perfonned to detennine best practices of preventing sleep deprivation in shift nurses. Literature searches using CINAHL, Medline, Medline Plus, PubMed, Cochrane Library, …

Gasification Of Biomass, Coal, And Petroleum Coke At High Heating Rates And Elevated Pressure, Aaron D. Lewis

Theses and Dissertations

Gasification is a process used to convert any carbonaceous species through heterogeneous reaction to obtain the desired gaseous products of H2 and CO which are used to make chemicals, liquid transportation fuels, and power. Both pyrolysis and heterogeneous gasification occur in commercial entrained-flow gasifiers at pressures from 4 to 65 atm with local gas temperatures as high as 2000 °C. Many gasification studies have been performed at moderate temperatures, heating rates, and pressures. In this work, both pyrolysis and char gasification experiments were performed on coal, petroleum coke, and biomass at conditions pertinent to commercial entrained-flow gasifiers. Rapid biomass pyrolysis …

Voice Features Of Sjogren's Syndrome: Examination Of Relative Fundamental Frequency (Rff) During Connected Speech, Victoria Anne Lovett

Theses and Dissertations

The purpose of this study was to examine the effectiveness of relative fundamental frequency (RFF) in quantifying voice disorder severity and possible change with treatment in individuals with Primary Sjögren's Syndrome (SS). Participants completed twice-daily audio recordings during an ABAB within-subjects experimental study investigating the effects of nebulized saline on voice production in this population. Voice samples of the Rainbow Passage from seven of the eight individuals with Primary SS involved in a larger investigation met inclusion criteria for analysis, for a total of 555 tokens. The results indicated that RFF values for this sample were similar to previously reported …

Doctor Who And The Creation Of A Non-Gendered Hero Archetype, Alessandra J. Pelusi

Theses and Dissertations

This project investigates the ways in which the television program Doctor Who creates a new, non-gendered hero archetype.

The Relationship Between Environmental Factors And Cyanobacteria Population In Lake Bloomington And Evergreen Lake In Mclean County, Illinois, Matthew Douglas Meyers

Theses and Dissertations

This thesis investigates cyanobacteria on two lakes in McLean County, Illinois from the months of August to October. Data was collected every other week.Cyanobacteria are prokaryotes that may be unicellular, colonial or filamentous in form. These cells can be known to be toxic or non-toxic. The factors that control the reproduction of cyanobacteria are the temperature and nutrients of nitrogen and phosphorus. These microorganisms are located in marine and terrestrial settings worldwide. The focus of this study was at two man-made lakes which are Lake Bloomington and Lake Evergreen. Sampling and collection of data was done on two lakes to …

Effects Of Genetic Modification Of Phosphoinositol Pathway On Light Signaling And Secondary Metabolism In Tomato Plants, Mohammad Alimohammadi

Theses and Dissertations

Inositol- (1,4,5) triphosphate (InsP3) is known as an important signaling factor and a regulator of plant responses to environmental and stress conditions. Overexpression of the InsP 5-ptase gene is found to enhance tolerance of transgenic tomato plants to abiotic stress and the induction of lycopene synthesis in InsP 5-ptase transgenic plants. Here, we demonstrated that the genetic reduction of InsP3 affected the overall phosphoinositol pathway, flavonoid metabolism, and light signaling in InsP 5-ptase transgenic plants. Transgenic plants that overexpress the InsP 5-ptase gene demonstrate an increase in the expression of major light signaling transcription factors (LeHY5, SIMYB12, LeELIP). We found …

The Legacy Of Alexander Bain, Stephen Lindsay Satterfield

Theses and Dissertations

Alexander Bain's key works are reviewed once again from a rhetorical and historical perspective. Bain was a Scottish empiricist who lived during the Industrial Revolution and contributed to rhetoric, psychology, education, and logic. Bain's emphasis on contiguity, modes of discourse, and grammar are explained. A brief Bain biography is provided along with Bain's interactions with notable friends. Researching Bain again from an interdisciplinary perspective is undertaken to avoid the usual problems with simply repeating prior academic findings and set opinions. However, the way in which Bain was accepted and then discredited is also discussed.

Genetic Analysis Of Ton2 Dependent Processes In Microtubule Organization In Arabidopsis Thaliana, Samantha Nicole Atkinson

Theses and Dissertations

Microtubules carry out many functions within the cell. They're used during mitosis and meiosis to move chromosomes to opposite sides of the cell so that cell division can occur. They're also used as structural support for the cell and are involved in establishing the cell shape. Microtubules are also able to reorient from a longitudinal orientation to a transverse orientation in response to gibberellic acid and auxin (two hormones that are involved in cell elongation). The mechanism for how this reorientation occurs is unknown. The TONNEAU2 (TON2) protein is necessary for proper microtubule organization. We looked at how TON2 affects …

The Wandering Spider Guild Of Webb County, Texas, Monica Trevino

Theses and Dissertations

Analysis of 10 years of spider collecting in Webb County, Texas has yielded 549 specimens in 21 genera and 55 species. This study was performed to establish similarities and relationships among wandering spiders of south Texas in Webb County and to identify the members of the wandering spider guild. The families included in this guild are the Clubionidae, Corinnidae, Gnaphosidae, Liocranidae, and Miturgidae. Wet pitfall traps and hand collecting were utilized in the gathering of the specimens. Spiders were identified using the most recent literature and allowed for an examination of the interactions of the members of this guild based …

Manipulation Of Light In Plasmonic Nano-Structures, Rehab Kotb Abd-Allah

Theses and Dissertations

Manipulating light at nano-scale is usually shadowed by the diffraction limit. Recently, plasmonics have emerged as a new technology that enables confining light at nano-scale. Using plasmonic structures, photonic devices can be shrunk from the micro-scale to the nano-scale. In this thesis, a novel structure to a plasmonic nano-filter is introduced and analyzed. The proposed nano-resonator has low loss, compact size and good sensing characteristics. A closed form model to the filter behavior is developed. The model is extracted from the waveguide physical parameters and provides a physical insight into the structure of the filter. An analytical model to the …

Pharmacologic Immunomodulation Of Macrophage Activation By Caffeine, Ryan Perry Steck

Theses and Dissertations

Caffeine is one of the most widely used neurostimulants in the world and there is considerable debate on its effect in immune cells. One of its main targets is proposed to be adenosine receptors which mediate an anti-inflammatory switch in activated immune cells while another target is phosphodiesterase where it acts as an inhibitor. In macrophages, caffeine has been shown to cause both pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes. If the primary effect of caffeine on macrophages were to antagonize adenosine receptors we would expect cells exposed to caffeine to have a prolonged M1 response. However, we show that caffeine …

From The Forefront To The Sidelines: Youth In Post-Revolution Egypt, Khadiga Omar

Theses and Dissertations

This thesis examines the reasons for the declining role of youth as effective political players since the Egyptian Revolution in 2011. Particularly looking at the “Revolutionary Youth”, this study investigates why their activism over the course of the past four years could not translate into political power that would lead to the realization of the goals of the revolution. Resource Mobilization Theory, Political Opportunity Structure and Framing constitute a tri-lens through which the political experience of the youth is analyzed in this research. This thesis argues that a) the youth’s organizational dilemma; b) their reliance on protest as the exclusive …

Novel On-Chip Applications Using Silicon Photonics, Rania Gamal

Theses and Dissertations

The emerging field of silicon photonics offers solutions to designing CMOScompatible optical devices. By taking advantage of the immense fabrication infrastructure offered by the silicon industry, it would be possible to design optical structures that are smaller, faster, less-power consuming and cheaper than traditional, non-silicon-based optical devices. In this dissertation, the design and performance testing of two novel silicon photonic structures are presented: 1) Silicon nanowire ridge waveguide for sensing applications at the optical transmission frequency. 2) Doped silicon plasmonic structures for negative-index, epsilon-near-zero and sensing applications at the mid-infrared. For the first design, silicon nanowires are arranged in a …

Characterization Of Postural Tremor In Essential Tremor Using A Seven-Degree-Of-Freedom Model, Daniel William Geiger

Theses and Dissertations

Essential Tremor (ET), a condition characterized by postural and kinetic tremor in the upper limbs, is one of the most prevalent movement disorders. While pharmaceutical and surgical treatment options exist, they are not ideal. Assistive devices have the potential to provide relief to patients but are largely unexplored for ET. Furthermore, prior characterizations of essential tremor have focused on endpoint tremor and provide insufficient detail for designing such a device. We propose and demonstrate a novel method for characterizing essential tremor in the 7 proximal degrees of freedom (DOF) of the upper limb in various postures. In addition, we provide …

Towards A Dynamic View Of Genetic Networks: A Kalman Filtering Framework For Recovering Temporally-Rewiring Stable Networks From Undersampled Data, Jehandad Khan

Theses and Dissertations

It is widely accepted that cellular requirements and environmental conditions dictate the architecture of genetic regulatory networks. Nonetheless, the status quo in regulatory network modeling and analysis assumes an invariant network topology over time. We refocus on a dynamic perspective of genetic networks, one that can uncover substantial topological changes in network structure during biological processes such as developmental growth and cancer progression. We propose a novel outlook on the inference of time-varying genetic networks, from a limited number of noisy observations, by formulating the networks estimation as a target tracking problem. Assuming linear dynamics, we formulate a constrained Kalman …

Reflections Of Self: Images Of People With Specific Learning Disabilities And Attention Deficit / Hyperactive Disorder In Children's Literature, Elizabeth R. Hayes

Theses and Dissertations

In this dissertation, I explored the portrayals of people with a specific learning disability or with attention deficit/hyperactivity disorder in children's literature. Eighteen books were analyzed to determine what themes and messages were being conveyed to children.

The major themes were about friendship, and overcoming issues associated with a SLD or AD/HD. In addtion, the portrayals suggest that children with SLD and AD/HD have similar problems like their peers with these conditions. However, there were portrayals of stereotypical roles and usage of negative language.